CN110433114A - The plant extracts inhibited tyrosinase activity and its application - Google Patents
The plant extracts inhibited tyrosinase activity and its application Download PDFInfo
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9771—Ginkgophyta, e.g. Ginkgoaceae [Ginkgo family]
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9783—Angiosperms [Magnoliophyta]
- A61K8/9789—Magnoliopsida [dicotyledons]
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/02—Preparations for care of the skin for chemically bleaching or whitening the skin
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- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
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- A61K2800/59—Mixtures
- A61K2800/592—Mixtures of compounds complementing their respective functions
- A61K2800/5922—At least two compounds being classified in the same subclass of A61K8/18
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/74—Biological properties of particular ingredients
- A61K2800/78—Enzyme modulators, e.g. Enzyme agonists
- A61K2800/782—Enzyme inhibitors; Enzyme antagonists
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Abstract
The invention discloses a kind of plant extracts inhibited tyrosinase activity and its applications.The plant extracts is ginkgo biloba extract, pomegranate rind extract, Pyracantha extract, Angelica Dahurica extract, small japanese bearbind extract.Plant extracts single dose prepared by the present invention all has the effect for inhibiting B16 cell in melanoma cells, wherein, it was found that ginkgo biloba extract and pomegranate rind extract and Pyracantha extract and small japanese bearbind extract are used in mixed way, it has obvious synergistic function, can be used as the active raw material for preparing skin-lightening cosmetic.
Description
Technical field
The invention belongs to technical field of cosmetic preparation, and in particular to a kind of plant extracts inhibited tyrosinase activity
And its application.
Background technique
The color of human skin depends on the content and distribution of melanin, and the physiology course that melanin is formed substantially can be general
Include as 3 stages: melanocyte generates melanin；Melanin granule passes through melanocyte dendritic processes to epithelial cells
Transfer；The melanin granule for being transferred to epithelial cells goes upward to cuticula with epidermal cell, and falls off and drain with cuticula.
Tyrosinase is the key enzyme of organism synthesis of melanin, and the main function of common skin-lightening cosmetic is to influence
The metabolism of tyrosinase, melanocyte and melanin.It has now been found that, various plants extract contains inhibition tyrosinase
Active substance, such as pitaya fruit, herba stellariae mediae, Stenoloma chusana, stringy stonecrop, humulus grass aerial part, big green handle mulberry, Chinese gall insect gall, setose thistle
Herb, black rice chaff, active material are proved to effectively have arbutin, glutathione, chitooligosaccharide-, and oxidized resveratrol etc. removes
Other than arbutin is significant in efficacy, other several known bioactive molecule effects are poor, and the arbutin yield of industrialized production
Low, side reaction is more, generates the chemical substance unfavorable to human body.
In vivo, the small molecule metabolites complex inhibited tyrosinase activity, often there are many conformations, different
The small molecule active of conformation is different, and the active small molecular that most of plant extracts contains is indefinite, and activity is lower, combines several
Mutual antagonism often occurs for kind existing plant extracts, and activity is lower, finds and inhibits tyrosinase activity high plant and mention
Object or several plant extracts being mutually improved are taken, is the difficult point studied in the art.
Summary of the invention
The purpose of the present invention is to provide a kind of plant extracts inhibited tyrosinase activity.
The present invention also aims to the synergistic plant extracts combination of energy is chosen from above-mentioned plant extracts.
A kind of plant extracts inhibited tyrosinase activity, the plant extracts is ginkgo biloba extract, granatum mentions
Take object, Pyracantha extract, Angelica Dahurica extract, small japanese bearbind extract.
The plant extracts is the mixture that ginkgo biloba extract and pomegranate rind extract are mixed according to the mass ratio of 1:1.
The plant extracts is the mixture that Pyracantha extract and small japanese bearbind extract are mixed according to the mass ratio of 1:1.
The extracting method of the ginkgo biloba extract are as follows: choose dry ginkgo leaf, crush, cross 80-120 mesh, 5-8 is added
70% alcohol reflux again extracts 3 times, merging filtrate, and vacuum distillation recycling ethyl alcohol, residual filtrate crosses large pore resin absorption column color
Spectrum collects 75% ethanol elution part, is dried under reduced pressure, is made.
The extracting method of the pomegranate rind extract are as follows: choose dry granatum, crush, cross 60-80 mesh, 6- is added
9 times of 70% alcohol reflux extracts 3 times, merging filtrate, and vacuum distillation recycling ethyl alcohol, residual filtrate crosses large pore resin absorption column color
Spectrum collects 75% ethanol elution part, is dried under reduced pressure, is made.
The extracting method of the Pyracantha extract are as follows: pyracantha fortuneana dry fruit is crushed, is sieved, 30-60 mesh pyracantha fortuneana powder is made；
The ratio that water and ethyl alcohol volume ratio are 3:6 prepares extracting solution；In gram, extracting solution is in terms of milliliter, by solid for correct amount angelica dahurica powder
Pyracantha fortuneana powder is soaked in extracting solution 48 hours by liquor ratio 1:8 in terms of grams per milliliter；Refluxing extraction 1 hour, stopping was extracted wait cool down
Heat up extraction 1 time again after to room temperature, and Extracting temperature is no more than 80 DEG C；It is filtered by vacuum after cooling, filtrate will be molten with Rotary Evaporators
Agent obtains Pyracantha extract after steaming.
The extracting method of the Angelica Dahurica extract are as follows: root of Dahurain angelica dry root is crushed, is sieved, 20-40 mesh angelica dahurica powder is made；Water
With the ratio preparation extracting solution that ethyl alcohol volume ratio is 3:7；In gram, extracting solution is in terms of milliliter, by solid-liquid for correct amount angelica dahurica powder
Than the 1:10 in terms of grams per milliliter, angelica dahurica powder is soaked in extracting solution 24 hours；Refluxing extraction 1 hour, stopping was extracted wait be cooled to
Heat up extraction 1 time again after room temperature, and Extracting temperature is no more than 80 DEG C；It is filtered by vacuum after cooling, filtrate is with Rotary Evaporators by solvent
Angelica Dahurica extract is obtained after steaming.
The extracting method of the small japanese bearbind extract are as follows: dry small japanese bearbind cauline leaf is chosen, is crushed, 80-100 mesh is crossed,
5-8 times of 70% alcohol reflux is added to extract 3 times, merging filtrate, vacuum distillation recycling ethyl alcohol, residual filtrate crosses macroporous absorption tree
Rouge column chromatography collects 75% ethanol elution part, is dried under reduced pressure, is made.
A kind of face cream includes above-mentioned plant extracts.
The face cream the preparation method is as follows:
(1) plant extracts 5-8g accurately is weighed, be put into beaker；
(2) it measures 100ml distilled water to pour into beaker, is put into magnetite, 1.5h is dissolved by heating in magnetic stirring apparatus；
(3) it after the completion of dissolving by heating, first filters once, rear filtering is primary, obtains plant extracts clarified solution；
(4) another clean beaker is taken, 20ml plant extracts clarified solution is measured with graduated cylinder and pours into beaker, add 200ml
Distilled water, stirring；
(5) 4g emulsifier, 2g glycerol are weighed, 10ml dipropylene glycol is measured and pours into beaker together, stir 5min；
(6) 100uL PCA-Na, 10uL essence are instilled, 5min is stirred；
(7) it packs.
Beneficial effects of the present invention: ginkgo biloba extract prepared by the present invention, pomegranate rind extract, Pyracantha extract, the root of Dahurain angelica
Extract and small japanese bearbind extract, single dose all have the effect for inhibiting B16 cell in melanoma cells, wherein discovery silver
Apricot extract and pomegranate rind extract and Pyracantha extract and small japanese bearbind extract are used in mixed way, and are had apparent synergistic
Effect, can be used as the active raw material for preparing skin-lightening cosmetic.
Specific embodiment
The present invention will be further described combined with specific embodiments below.
Plant extracts is prepared with the following method in following embodiments:
The extracting method of ginkgo biloba extract: choosing dry ginkgo leaf, crushes, sieves with 100 mesh sieve, and 6 times of 70% second is added
Alcohol reflux extracts 3 times, merging filtrate, and vacuum distillation recycling ethyl alcohol, residual filtrate crosses macroporous absorbent resin column chromatography, collects 75%
Ethanol elution part, is dried under reduced pressure, and is made.
The extracting method of pomegranate rind extract: choosing dry granatum, crushes, and crosses 70 meshes, and 8 times of 70% second is added
Alcohol reflux extracts 3 times, merging filtrate, and vacuum distillation recycling ethyl alcohol, residual filtrate crosses macroporous absorbent resin column chromatography, collects 75%
Ethanol elution part, is dried under reduced pressure, and is made.
The extracting method of Pyracantha extract: pyracantha fortuneana dry fruit is crushed, and 40 mesh pyracantha fortuneana powder are made in sieving；Water and ethyl alcohol
The ratio that volume ratio is 3:6 prepares extracting solution；Correct amount angelica dahurica powder in gram, extracting solution in terms of milliliter, by solid-to-liquid ratio with gram/
Milliliter meter 1:8, pyracantha fortuneana powder is soaked in extracting solution 48 hours；Refluxing extraction 1 hour, stopping was extracted after being cooled to room temperature again
Heating is extracted 1 time, and Extracting temperature is no more than 80 DEG C；It is filtered by vacuum after cooling, filtrate obtains after being steamed solvent with Rotary Evaporators
To Pyracantha extract.
The extracting method of Angelica Dahurica extract: root of Dahurain angelica dry root is crushed, and 30 mesh angelica dahurica powders are made in sieving；Water and ethyl alcohol body
Product prepares extracting solution than the ratio for being 3:7；Correct amount angelica dahurica powder in gram, extracting solution in terms of milliliter, by solid-to-liquid ratio with gram/milli
Meter 1:10 is risen, angelica dahurica powder is soaked in extracting solution 24 hours；Refluxing extraction 1 hour, stopping was extracted after being cooled to room temperature again
Heating is extracted 1 time, and Extracting temperature is no more than 80 DEG C；It is filtered by vacuum after cooling, filtrate obtains after being steamed solvent with Rotary Evaporators
To Angelica Dahurica extract.
The extracting method of small japanese bearbind extract: choosing dry small japanese bearbind cauline leaf, crushes, sieves with 100 mesh sieve, and is added 6 times
70% alcohol reflux extracts 3 times, merging filtrate, and vacuum distillation recycling ethyl alcohol, residual filtrate is crossed macroporous absorbent resin column chromatography, received
Collect 75% ethanol elution part, is dried under reduced pressure, is made.
Agents useful for same and instrument in following embodiments:
Arbutin (sigma company)；B16 mouse black-in tumor cell is purchased from Chinese Academy of Sciences Shanghai Institute of Cell Biology；
RPMI-1640 fluid nutrient medium (containing HEPES, dual anti-), 4 DEG C of refrigerators save；96 well culture plates, 24 well culture plates, 6
Well culture plate；Tally；The sterile filter of 0.20 μ, cryopreservation tube, EP pipe；Pipette；Sample loading gun (Thermo)；Centrifuge tube；Oscillation is mixed
Close instrument；Microplate reader；Homogenizer；Table-type high-speed refrigerated centrifuge (ShangHai City centrifugal Machine Institute)；Electronic scale；Water bath with thermostatic control
Case；Cell counter；Visible spectrophotometer (ShangHai City centrifugal Machine Institute).
Influence of 1 plant extracts of embodiment to Melanoma Cell Proliferation
Each plant extracts sample is diluted to the dense of 6.25,12.5,25,50,100 μ g/mL with fluid nutrient medium respectively
Degree, and with 0.20 μm of sterile filter filter after it is spare.
The B16 mouse black-in tumor cell for selecting logarithmic growth phase, it is complete with RPMI-1640 after the digestion of 0.25% pancreatin
It is 1 × 10 that culture medium, which is made into density,5A cell suspension is inoculated in 96 well culture plates, every 100 μ L of hole, in 37 DEG C, 5%CO2Training
Support culture in case.After adherent, it is separately added into the complete culture solution of the sample containing various concentration, control group adds isometric cell outstanding
Liquid, and set blank control group and add isometric complete medium, every 100 μ L of hole.Each concentration sets 3 multiple holes, 37 DEG C, 5%CO2
In incubator after culture 72h, mtt assay measures absorbance (OD) value at microplate reader 570nm.It is opposite that cell is calculated according to the following formula
Proliferation rate:
Cell opposite proliferation rate=[(A-C)/(B-C)] × 100%
Wherein A is each concentration mean absorbance values of object to be sieved, and B is control group mean absorbance values, and C is that blank group is averagely inhaled
Shading value.Test result is shown in Table 1:
Opposite proliferation rate of 15 Plant Extracts of table to melanoma cells
As can be seen from Table 1, as the arbutin of positive control when dosage is 100 μ g/mL, to the phase of melanoma cells
It is 85.31% to proliferation rate, ginkgo biloba extract faint to B16 mouse black-in tumor cell toxicity, pomegranate rind extract, pyracantha fortuneana mentions
Take object, Angelica Dahurica extract, small japanese bearbind extract is fainter to B16 mouse black-in tumor cell toxicity.
B16 melanoma cells strain is a kind of gland cell with dendrite, since dendrite is that melanoma cells are defeated
The channel of melanin granule is sent, while being also the bridge contacted with keratinocyte, therefore, the upgrowth situation of melanoma cells
Whether normal, the performance of melanoma cells function plays a significant role.This experiment confirms plant extracts of the invention to B16
Melanoma cells toxic effect is very small, also further illustrates to cell fanout free region for normal somatic cell also just there are no evil,
Plant extracts of the invention is not to block B16 cell in melanoma cells, Jin Erda by inhibiting cel l proliferation
Arrive whitening effect.
Influence of 2 plant extracts of embodiment to B16 cell in melanoma cells
By the 3rd generation melanoma cells of culture with 1 × 104A density connects hole in 6 orifice plates, changes liquid afterwards for 24 hours.Add test sample
3 holes of every kind of drug of product, sample concentration is the same as embodiment 1.Control group replaces sample solution with RPMI1640 culture solution.37 DEG C, 5%
CO272h after incubation, discards supernatant liquid.Every hole is added 2.5g/L pancreatin and digests 5min at room temperature.4mL culture solution is added to stop
Digestion, and blow and beat into single cell suspension.0.5mL is taken to make cell count, remaining cell suspension 1500r/min is centrifuged 10min, in abandoning
Clearly, the NaOH of 1mL1mol/L is added, and surveys its absorbance value at microplate reader 490nm after incubation 2h in 90 DEG C of water-baths.It presses
Following equation calculates B16 cell amount and drug to the inhibiting rate of B16 cell.
Melanin genesis inhibiting rate=[1- (sample well absorbance value/sample well cell density)/(control wells absorbance value/right
According to hole cell density) × 100%, test result is shown in Table 2:
Inhibiting effect of 25 Plant Extracts of table to melanoma cells B16 cell
As can be seen from Table 2, arbutin, pomegranate rind extract, small japanese bearbind extract close B16 melanoma cells melanin
At inhibiting effect it is apparent that sample concentration be 100 μ g/mL when, the inhibiting rate of B16 cell is respectively reached
33.48%, 30.82% and 38.92%, ginkgo biloba extract, Pyracantha extract, Angelica Dahurica extract is also inhibited, but presses down
It is weaker to make use.
It chooses above-mentioned plant extracts to mix two-by-two, concentration is 50 μ g/mL, repeats above-mentioned melanoma cells melanin and closes
At Inhibition test, experimental result is shown in Table 3:
Inhibiting effect of the 3 mixed plant extract of table to melanoma cells B16 cell
As can be seen from Table 3, most of mixed plant extract weakens with respect to the inhibiting effect of single dose, is in antagonism,
Ginkgo biloba extract and pomegranate rind extract are applied in combination, and Pyracantha extract and small japanese bearbind extract combination use, and inhibiting effect increases
By force, synergy is shown as.
It is well known that tyrosinase is the key enzyme of synthesis of melanin in organism, the metalloenzyme of this cupric can be urged
Change single phenol hydroxylation to be oxidized to quinone (o-diphenolase activity) at diphenol (single phenol enzymatic activity), and diphenol, quinone shape under the conditions of non-enzymatic
At final reaction product melanin, it is mutual by influencing tyrosinase, dopachrome that plant extracts, which inhibits B16 cell,
Become the expression and activity or other mechanism not understood for us of enzyme and dihydroxy indole carboxylic oxidase etc., finally
Achieve the purpose that skin whitening.
Embodiment 3
Face cream, preparation method are as follows:
(1) ginkgo biloba extract 3g accurately is weighed, pomegranate rind extract 3g is put into beaker；
(2) it measures 100ml distilled water to pour into beaker, is put into magnetite, 1.5h is dissolved by heating in magnetic stirring apparatus；
(3) it after the completion of dissolving by heating, first filters once, rear filtering is primary, obtains plant extracts clarified solution；
(4) another clean beaker is taken, 20ml plant extracts clarified solution is measured with graduated cylinder and pours into beaker, add 200ml
Distilled water, stirring；
(5) 4g emulsifier, 2g glycerol are weighed, 10ml dipropylene glycol is measured and pours into beaker together, stir 5min；
(6) 100uL PCA-Na, 10uL essence are instilled, 5min is stirred；
(7) it packs.
Embodiment 4
Face cream, preparation method are as follows:
(1) Pyracantha extract 3g accurately is weighed, small japanese bearbind extract 3g is put into beaker；
(2) it measures 100ml distilled water to pour into beaker, is put into magnetite, 1.5h is dissolved by heating in magnetic stirring apparatus；
(3) it after the completion of dissolving by heating, first filters once, rear filtering is primary, obtains plant extracts clarified solution；
(4) another clean beaker is taken, 20ml plant extracts clarified solution is measured with graduated cylinder and pours into beaker, add 200ml
Distilled water, stirring；
(5) 4g emulsifier, 2g glycerol are weighed, 10ml dipropylene glycol is measured and pours into beaker together, stir 5min；
(6) 100uL PCA-Na, 10uL essence are instilled, 5min is stirred；
(7) it packs.
Claims (10)
1. a kind of plant extracts inhibited tyrosinase activity, which is characterized in that the plant extracts be ginkgo biloba extract,
Pomegranate rind extract, Pyracantha extract, Angelica Dahurica extract, small japanese bearbind extract.
2. the plant extracts inhibited tyrosinase activity according to claim 1, which is characterized in that the plant extracts
The mixture mixed for ginkgo biloba extract and pomegranate rind extract according to the mass ratio of 1:1.
3. the plant extracts inhibited tyrosinase activity according to claim 1, which is characterized in that the plant extracts
The mixture mixed for Pyracantha extract and small japanese bearbind extract according to the mass ratio of 1:1.
4. the plant extracts inhibited tyrosinase activity according to claim 1, which is characterized in that the ginkgo biloba extract
Extracting method are as follows: choose dry ginkgo leaf, crush, cross 80-120 mesh, be added 5-8 times 70% alcohol reflux extraction 3
Secondary, ethyl alcohol is recycled in merging filtrate, vacuum distillation, and residual filtrate crosses macroporous absorbent resin column chromatography, collects 75% ethanol elution portion
Point, it is dried under reduced pressure, is made.
5. the plant extracts inhibited tyrosinase activity according to claim 1, which is characterized in that the granatum extracts
The extracting method of object are as follows: choose dry granatum, crush, cross 60-80 mesh, 6-9 times of 70% alcohol reflux is added and extracts 3
Secondary, ethyl alcohol is recycled in merging filtrate, vacuum distillation, and residual filtrate crosses macroporous absorbent resin column chromatography, collects 75% ethanol elution portion
Point, it is dried under reduced pressure, is made.
6. the plant extracts inhibited tyrosinase activity according to claim 1, which is characterized in that the Pyracantha extract
Extracting method are as follows: pyracantha fortuneana dry fruit is crushed, be sieved, 30-60 mesh pyracantha fortuneana powder is made；Water and ethyl alcohol volume ratio are 3:6's
Ratio prepares extracting solution；In gram, extracting solution, will by solid-to-liquid ratio 1:8 in terms of grams per milliliter in terms of milliliter for correct amount angelica dahurica powder
Pyracantha fortuneana powder is soaked in extracting solution 48 hours；Refluxing extraction 1 hour, the extraction 1 time that heats up again after being cooled to room temperature was extracted in stopping,
Extracting temperature is no more than 80 DEG C；It is filtered by vacuum after cooling, filtrate obtains Pyracantha extract after being steamed solvent with Rotary Evaporators.
7. the plant extracts inhibited tyrosinase activity according to claim 1, which is characterized in that the Angelica Dahurica extract
Extracting method are as follows: root of Dahurain angelica dry root is crushed, be sieved, 20-40 mesh angelica dahurica powder is made；The ratio that water and ethyl alcohol volume ratio are 3:7
Example preparation extracting solution；In gram, extracting solution, will be white by solid-to-liquid ratio 1:10 in terms of grams per milliliter in terms of milliliter for correct amount angelica dahurica powder
Root of Dahurian angelica powder is soaked in extracting solution 24 hours；Refluxing extraction 1 hour, the extraction 1 time that heats up again after being cooled to room temperature was extracted in stopping, being mentioned
Temperature is taken to be no more than 80 DEG C；It is filtered by vacuum after cooling, filtrate obtains Angelica Dahurica extract after being steamed solvent with Rotary Evaporators.
8. the plant extracts inhibited tyrosinase activity according to claim 1, which is characterized in that the small japanese bearbind extracts
The extracting method of object are as follows: choose dry small japanese bearbind cauline leaf, crush, cross 80-100 mesh, 5-8 times of 70% alcohol reflux is added
It extracts 3 times, merging filtrate, vacuum distillation recycling ethyl alcohol, residual filtrate crosses macroporous absorbent resin column chromatography, collects 75% ethyl alcohol and washes
De- part, is dried under reduced pressure, is made.
9. a kind of face cream, which is characterized in that include plant extracts described in claim 1.
10. face cream according to claim 9, which is characterized in that the face cream the preparation method is as follows:
(1) plant extracts 5-8g accurately is weighed, be put into beaker；
(2) it measures 100ml distilled water to pour into beaker, is put into magnetite, 1.5h is dissolved by heating in magnetic stirring apparatus；
(3) it after the completion of dissolving by heating, first filters once, rear filtering is primary, obtains plant extracts clarified solution；
(4) another clean beaker is taken, 20ml plant extracts clarified solution is measured with graduated cylinder and pours into beaker, adds 200ml distillation
Water, stirring；
(5) 4g emulsifier, 2g glycerol are weighed, 10ml dipropylene glycol is measured and pours into beaker together, stir 5min；
(6) 100uL PCA-Na, 10uL essence are instilled, 5min is stirred；
(7) it packs.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111388367A (en) * | 2020-03-25 | 2020-07-10 | 江南大学 | Composition for inhibiting melanin at multiple target points, preparation method and application of composition in cosmetics |
| CN113318061A (en) * | 2021-06-16 | 2021-08-31 | 上海南滨江细胞生物科技有限公司 | A facial cream containing embryo extract and its preparation method |
| CN113476356A (en) * | 2021-04-30 | 2021-10-08 | 云南英格生物技术有限公司 | Preparation method and application of pyracantha fortuneana fruit extract |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH07238011A (en) * | 1994-02-24 | 1995-09-12 | Suntory Ltd | Skin-beautifying cosmetic composition |
| WO2009067095A1 (en) * | 2007-11-19 | 2009-05-28 | Stiefel Laboratories, Inc. | Topical cosmetic skin lightening compositions and methods of use thereof |
| CN103251659A (en) * | 2013-06-03 | 2013-08-21 | 青岛三九九洲生物技术有限公司 | Preparation method of ginkgo leaf essence |
| CN105434201A (en) * | 2014-09-25 | 2016-03-30 | 伽蓝（集团）股份有限公司 | Composition containing pomegranate extract and use thereof, and skin externally-applied agent |
| CN107384069A (en) * | 2017-06-30 | 2017-11-24 | 天长市巨龙车船涂料有限公司 | A kind of waterborne UV coating |
| CN109125150A (en) * | 2017-06-15 | 2019-01-04 | 新乡医学院三全学院 | A kind of skin care item and the preparation method and application thereof containing hawthorne leaf P.E |
-
2019
- 2019-08-16 CN CN201910759872.9A patent/CN110433114B/en active Active
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH07238011A (en) * | 1994-02-24 | 1995-09-12 | Suntory Ltd | Skin-beautifying cosmetic composition |
| WO2009067095A1 (en) * | 2007-11-19 | 2009-05-28 | Stiefel Laboratories, Inc. | Topical cosmetic skin lightening compositions and methods of use thereof |
| CN101917965A (en) * | 2007-11-19 | 2010-12-15 | 施泰福实验室股份有限公司 | Topical cosmetic skin lightening compositions and methods of use thereof |
| CN103251659A (en) * | 2013-06-03 | 2013-08-21 | 青岛三九九洲生物技术有限公司 | Preparation method of ginkgo leaf essence |
| CN105434201A (en) * | 2014-09-25 | 2016-03-30 | 伽蓝（集团）股份有限公司 | Composition containing pomegranate extract and use thereof, and skin externally-applied agent |
| CN109125150A (en) * | 2017-06-15 | 2019-01-04 | 新乡医学院三全学院 | A kind of skin care item and the preparation method and application thereof containing hawthorne leaf P.E |
| CN107384069A (en) * | 2017-06-30 | 2017-11-24 | 天长市巨龙车船涂料有限公司 | A kind of waterborne UV coating |
Non-Patent Citations (2)
| Title |
|---|
| 何蓉蓉等: "火棘果实提取物的美白作用", 《中国实验方剂学杂志》 * |
| 罗达尚，: "《晶珠本草正本诠释》", 11 September 1995, 四川科学技术出版社 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111388367A (en) * | 2020-03-25 | 2020-07-10 | 江南大学 | Composition for inhibiting melanin at multiple target points, preparation method and application of composition in cosmetics |
| CN113476356A (en) * | 2021-04-30 | 2021-10-08 | 云南英格生物技术有限公司 | Preparation method and application of pyracantha fortuneana fruit extract |
| CN113318061A (en) * | 2021-06-16 | 2021-08-31 | 上海南滨江细胞生物科技有限公司 | A facial cream containing embryo extract and its preparation method |
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