CN110433114B - Plant extract for inhibiting tyrosinase activity and application thereof - Google Patents
Plant extract for inhibiting tyrosinase activity and application thereof Download PDFInfo
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
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- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9771—Ginkgophyta, e.g. Ginkgoaceae [Ginkgo family]
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
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- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
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- A61K8/9789—Magnoliopsida [dicotyledons]
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/02—Preparations for care of the skin for chemically bleaching or whitening the skin
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- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
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- A61K2800/59—Mixtures
- A61K2800/592—Mixtures of compounds complementing their respective functions
- A61K2800/5922—At least two compounds being classified in the same subclass of A61K8/18
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/74—Biological properties of particular ingredients
- A61K2800/78—Enzyme modulators, e.g. Enzyme agonists
- A61K2800/782—Enzyme inhibitors; Enzyme antagonists
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Abstract
The invention discloses a plant extract for inhibiting tyrosinase activity and application thereof. The plant extract is semen Ginkgo extract, pericarpium Granati extract, pyracantha fortuneana extract, radix Angelicae Dahuricae extract, and flos Calystegiae Sepii extract. The plant extract single agent prepared by the invention has the effect of inhibiting the synthesis of melanin in melanoma cells, wherein the ginkgo extract and the pomegranate peel extract, the pyracantha fortuneana extract and the spiraea parviflora extract are found to be mixed for use, have obvious synergistic effect and can be used as an active substance raw material for preparing whitening cosmetics.
Description
Technical Field
The invention belongs to the technical field of cosmetic preparation, and particularly relates to a plant extract for inhibiting tyrosinase activity and application thereof.
Background
The color of human skin depends on the content and distribution of melanin, and the physiological process of melanin formation can be basically summarized into 3 stages: melanocytes produce melanin; the melanin granules are transferred to the keratin cells through the melanocyte dendrites; melanin granules transferred to keratin cells travel up the stratum corneum with the epidermal cells and are excreted as the stratum corneum falls off.
Tyrosinase is a key enzyme for synthesizing melanin by organisms, and the main function of the commonly used whitening cosmetics is to influence the metabolism of tyrosinase, melanocytes and melanin. At present, it is found that various plant extracts contain substances inhibiting tyrosinase activity, such as honey fruits, chickweed, black chives, sedum sarmentosum, overground parts of humulus scandens, aloeswood, gallnut, Japanese thistle herb, black rice bran, active substances which prove to be effective are arbutin, glutathione, chitooligosaccharide, oxyresveratrol and the like, besides the obvious curative effect of arbutin, other known active molecules have poor effect, the yield of industrially produced arbutin is low, side reactions are more, and chemical substances which are unfavorable for human bodies are generated.
In organisms, small molecule metabolites inhibiting tyrosinase activity are extremely complex, often have multiple conformations, and small molecules with different conformations have different activities, most plant extracts have undefined active small molecules and low activities, and when the plant extracts are combined with a plurality of existing plant extracts, mutual antagonism often occurs, the activities are lower, and finding plant extracts inhibiting tyrosinase activity highly or plant extracts synergistic with each other is a difficult point in the research in the field.
Disclosure of Invention
The invention aims to provide a plant extract for inhibiting tyrosinase activity.
The invention also aims to select the plant extract combination which can be synergized from the plant extracts.
A plant extract for inhibiting tyrosinase activity is prepared from semen Ginkgo extract, pericarpium Granati extract, fructus Pyracanthae extract, radix Angelicae Dahuricae extract, and flos Calystegiae Sepii extract.
The plant extract is a ginkgo extract and a pomegranate bark extract according to the weight ratio of 1:1 in a mass ratio of 1.
The plant extract is a pyracantha fortuneana extract and a bindweed extract according to the weight ratio of 1:1 in a mass ratio of 1.
The extraction method of the ginkgo biloba extract comprises the following steps: selecting dried folium Ginkgo, pulverizing, sieving with 80-120 mesh sieve, adding 5-8 times of 70% ethanol, reflux extracting for 3 times, mixing filtrates, distilling under reduced pressure to recover ethanol, subjecting the rest filtrate to macroporous adsorbent resin column chromatography, collecting 75% ethanol eluate, and drying under reduced pressure.
The extraction method of the pomegranate bark extract comprises the following steps: selecting dried pericarpium Granati, pulverizing, sieving with 60-80 mesh sieve, adding 6-9 times of 70% ethanol, reflux extracting for 3 times, mixing filtrates, distilling under reduced pressure to recover ethanol, subjecting the rest filtrate to macroporous adsorbent resin column chromatography, collecting 75% ethanol eluate, and drying under reduced pressure.
The extraction method of the pyracantha fortuneana extract comprises the following steps: pulverizing dried fructus Pyracanthae, sieving, and making into 30-60 mesh powder; preparing an extracting solution by using water and ethanol according to the volume ratio of 3: 6; accurately weighing pyracantha fortuneana powder in grams, taking an extracting solution in milliliters, taking a solid-to-liquid ratio in grams/milliliters as 1:8, and soaking the pyracantha fortuneana powder in the extracting solution for 48 hours; reflux extracting for 1 hr, stopping extraction, cooling to room temperature, heating for 1 time, and extracting at a temperature not higher than 80 deg.C; cooling, vacuum filtering, and evaporating the solvent from the filtrate with rotary evaporator to obtain pyracantha fortuneana extract.
The extraction method of the angelica dahurica extract comprises the following steps: pulverizing dried radix Angelicae Dahuricae, sieving, and making into 20-40 mesh radix Angelicae Dahuricae powder; preparing an extracting solution by using water and ethanol according to the volume ratio of 3: 7; accurately weighing radix Angelicae Dahuricae powder in g, extracting solution in ml, and soaking radix Angelicae Dahuricae powder in the extracting solution for 24 hr, wherein the solid-to-liquid ratio is 1:10 in g/ml; reflux extracting for 1 hr, stopping extraction, cooling to room temperature, heating for 1 time, and extracting at a temperature not higher than 80 deg.C; cooling, vacuum filtering, and evaporating the solvent from the filtrate with rotary evaporator to obtain radix Angelicae Dahuricae extract.
The extraction method of the Convolvulus arvensis extract comprises the following steps: selecting dried stem and leaf of Convolvulus, pulverizing, sieving with 80-100 mesh sieve, reflux-extracting with 5-8 times of 70% ethanol for 3 times, mixing filtrates, distilling under reduced pressure to recover ethanol, subjecting the rest filtrate to macroporous adsorbent resin column chromatography, collecting 75% ethanol eluate, and drying under reduced pressure.
A facial cream comprises the above plant extract.
The preparation method of the face cream comprises the following steps:
(1) accurately weighing 5-8g of plant extract, and placing into a beaker;
(2) weighing 100ml of distilled water, pouring into a beaker, adding a magnet, and heating and dissolving for 1.5 hours in a magnetic stirrer;
(3) after the heating and dissolving are finished, carrying out suction filtration once, and then carrying out filtration once to obtain a plant extract clarified liquid;
(4) taking another clean beaker, weighing 20ml of plant extract clear solution by using a measuring cylinder, pouring into the beaker, adding 200ml of distilled water, and stirring;
(5) weighing 4g of emulsifier and 2g of glycerol, weighing 10ml of dipropylene glycol, pouring into a beaker together, and stirring for 5 min;
(6) dripping 100uL PCA-Na and 10uL essence, and stirring for 5 min;
(7) and (6) packaging.
The invention has the beneficial effects that: the single agents of the ginkgo extract, the pomegranate bark extract, the pyracantha extract, the angelica dahurica extract and the spiraea parviflora extract prepared by the invention have the effect of inhibiting the synthesis of melanin in melanoma cells, wherein the ginkgo extract, the pomegranate bark extract, the pyracantha fortunei extract and the spiraea parviflora extract are found to be mixed for use, have obvious synergistic interaction effect and can be used as active substance raw materials for preparing whitening cosmetics.
Detailed Description
The present invention is further illustrated by the following specific examples.
The plant extracts in the following examples were prepared as follows:
the extraction method of the ginkgo extract comprises the following steps: selecting dried folium Ginkgo, pulverizing, sieving with 100 mesh sieve, adding 6 times of 70% ethanol, reflux extracting for 3 times, mixing filtrates, distilling under reduced pressure to recover ethanol, subjecting the rest filtrate to macroporous adsorbent resin column chromatography, collecting 75% ethanol eluate, and drying under reduced pressure.
The extraction method of the pomegranate bark extract comprises the following steps: selecting dried pericarpium Granati, pulverizing, sieving with 70 mesh sieve, adding 8 times of 70% ethanol, reflux extracting for 3 times, mixing filtrates, distilling under reduced pressure to recover ethanol, subjecting the rest filtrate to macroporous adsorbent resin column chromatography, collecting 75% ethanol eluate, and drying under reduced pressure.
The extraction method of the pyracantha fortuneana extract comprises the following steps: pulverizing dried fructus Pyracanthae, sieving, and making into powder of 40 mesh; preparing an extracting solution by using water and ethanol according to the volume ratio of 3: 6; accurately weighing pyracantha fortuneana powder in grams, taking an extracting solution in milliliters, taking a solid-to-liquid ratio in grams/milliliters as 1:8, and soaking the pyracantha fortuneana powder in the extracting solution for 48 hours; reflux extracting for 1 hr, stopping extraction, cooling to room temperature, heating for 1 time, and extracting at a temperature not higher than 80 deg.C; cooling, vacuum filtering, and evaporating the solvent from the filtrate with rotary evaporator to obtain pyracantha fortuneana extract.
The extraction method of the angelica dahurica extract comprises the following steps: pulverizing dried radix Angelicae Dahuricae, sieving, and making into 30 mesh radix Angelicae Dahuricae powder; preparing an extracting solution by using water and ethanol according to the volume ratio of 3: 7; accurately weighing radix Angelicae Dahuricae powder in g, extracting solution in ml, and soaking radix Angelicae Dahuricae powder in the extracting solution for 24 hr, wherein the solid-to-liquid ratio is 1:10 in g/ml; reflux extracting for 1 hr, stopping extraction, cooling to room temperature, heating for 1 time, and extracting at a temperature not higher than 80 deg.C; cooling, vacuum filtering, and evaporating the solvent from the filtrate with rotary evaporator to obtain radix Angelicae Dahuricae extract.
The extraction method of the Convolvulus arvensis extract comprises the following steps: selecting dried stem and leaf of Convolvulus, pulverizing, sieving with 100 mesh sieve, adding 6 times of 70% ethanol, reflux extracting for 3 times, mixing filtrates, distilling under reduced pressure to recover ethanol, subjecting the rest filtrate to macroporous adsorbent resin column chromatography, collecting 75% ethanol eluate, and drying under reduced pressure.
Reagents and apparatus used in the following examples:
arbutin (sigma corporation); b16 mouse melanoma cells were purchased from shanghai institute of cell biology, department of chinese;
RPMI-1640 liquid culture medium (containing HEPES and double antibody), and storing in refrigerator at 4 deg.C; 96-well culture plates, 24-well culture plates and 6-well culture plates; a counting plate; 0.20 mu sterile filter head, freezing tube and EP tube; a pipette; sample application gun (Thermo); centrifuging the tube; oscillating the mixer; an enzyme-labeling instrument; a homogenizer; bench high speed refrigerated centrifuge (Shanghai institute for centrifugal mechanics); an electronic scale; a constant temperature water bath tank; a cell counter; visible spectrophotometer (Shanghai institute for centrifugal mechanics).
Example 1 Effect of plant extracts on melanoma cell proliferation
Each plant extract sample was diluted with liquid medium to concentrations of 6.25, 12.5, 25, 50, 100. mu.g/mL, and filtered through a 0.20 μm sterile filter head for use.
Selecting logarithmic growth phaseB16 mouse melanoma cells, digested with 0.25% trypsin, prepared in RPMI-1640 complete medium at a density of 1X 105The cell suspension was inoculated into a 96-well plate at 100. mu.L/well in 37 ℃ with 5% CO2Culturing in an incubator. After adherence, complete culture medium containing samples of different concentrations was added, and an equal volume of cell suspension was added to the control group, and 100. mu.L of complete culture medium per well was added to the blank control group. Each concentration is provided with 3 multiple holes at 37 ℃ and 5% CO2After culturing for 72h in an incubator, the absorbance (OD) value is measured at 570nm of a microplate reader by an MTT method. The relative proliferation rate of the cells was calculated according to the following formula:
the relative cell proliferation rate was [ (A-C)/(B-C) ] 100%
Wherein A is the average absorbance value of each concentration of the substances to be screened, B is the average absorbance value of a control group, and C is the average absorbance value of a blank group. The test results are shown in table 1:
TABLE 15 relative proliferation rates of plant extracts on melanoma cells
As can be seen from table 1, when the dose of arbutin as a positive control is 100 μ g/mL, the relative proliferation rate of the arbutin on melanoma cells is 85.31%, and the arbutin has weak toxicity on melanoma cells of B16 mice, and the ginkgo extract, the pomegranate bark extract, the pyracantha extract, the angelica dahurica extract and the bindweed extract have weak toxicity on melanoma cells of B16 mice.
The B16 melanoma cell line is an glandular cell having a dendrite, and since the dendrite is a channel for the melanoma cell to transport melanin granules and is also a bridge for the connection with keratinocytes, the growth of the melanoma cell is normal or not, and the function of the melanoma cell plays an important role. The experiment proves that the plant extract has little toxic effect on B16 melanoma cells, has no harm to cells and is harmless to normal somatic cells, and further shows that the plant extract does not block the synthesis of melanin in the melanoma cells by inhibiting the cell proliferation effect so as to achieve the whitening effect.
Example 2 Effect of plant extracts on melanin synthesis in melanoma cells
Cultured melanoma cells of passage 3 at 1X 104And (5) connecting holes with 6-hole plates in a density mode, and changing the liquid after 24 hours. Test samples were added at 3 wells per drug and sample concentrations as in example 1. The control group replaced the sample solution with RPMI1640 culture solution. 37 ℃ and 5% CO2After 72h incubation, the supernatant was discarded. Adding 2.5g/L pancreatin per well, and digesting at room temperature for 5 min. Digestion was stopped by adding 4mL of culture medium and pipetting to a single cell suspension. Taking 0.5mL as cell count, centrifuging the rest cell suspension at 1500r/min for 10min, discarding the supernatant, adding 1mL of 1mol/L NaOH, incubating in 90 deg.C water bath for 2h, and measuring the absorbance at 490nm of an enzyme labeling instrument. The melanin synthesis amount and the inhibition rate of the drug on the melanin synthesis are calculated according to the following formulas.
Melanin synthesis inhibition rate ═ 1- (sample well absorbance value/sample well cell density)/(control well absorbance value/control well cell density) × 100%, the test results are shown in table 2:
TABLE 25 inhibition of melanoma cell melanin synthesis by plant extracts
As can be seen from table 2, the inhibitory effect of arbutin, pomegranate bark extract and Convolvulus racemosus extract on the synthesis of melanin of B16 melanoma cells is very obvious, and the inhibitory rate on the synthesis of melanin reaches 33.48%, 30.82% and 38.92% respectively at a sample concentration of 100 μ g/mL, and the inhibitory effect of ginkgo biloba extract, pyracantha fortuneana extract and dahurian angelica root extract is also weak.
Selecting the plant extracts, mixing the plant extracts two by two, wherein the concentration of the plant extracts is 50 mu g/mL, repeating the experiment for inhibiting the synthesis of the melanin of the melanoma cells, and the experimental results are shown in a table 3:
TABLE 3 inhibitory Effect of Mixed plant extracts on the Melanin Synthesis of melanoma cells
As can be seen from table 3, most of the mixed plant extracts have a reduced inhibitory effect and an antagonistic effect with respect to the single agent, and the combined use of the ginkgo biloba extract and the pomegranate bark extract and the combined use of the pyracantha fortuneana extract and the bindweed extract have an enhanced inhibitory effect and show a synergistic effect.
It is known that tyrosinase is a key enzyme for synthesizing melanin in organisms, the copper-containing metalloenzyme can catalyze the hydroxylation of monophenol into diphenol (monophenolase activity) and oxidize the diphenol into quinone (diphenolase activity), the quinone forms the final reaction product melanin under non-enzymatic conditions, and the plant extract can inhibit the synthesis of the melanin by influencing the expression level and activity of tyrosinase, dopachrome tautomerase, dihydroxyindole carboxylate oxidase and the like, or other mechanisms which are not understood by people, so as to finally achieve the purpose of whitening the skin.
Example 3
The preparation method of the face cream comprises the following steps:
(1) accurately weighing 3g of ginkgo extract and 3g of pomegranate peel extract, and putting the ginkgo extract and the pomegranate peel extract into a beaker;
(2) weighing 100ml of distilled water, pouring into a beaker, adding a magnet, and heating and dissolving for 1.5 hours in a magnetic stirrer;
(3) after the heating and dissolving are finished, carrying out suction filtration once, and then carrying out filtration once to obtain a plant extract clarified liquid;
(4) taking another clean beaker, weighing 20ml of plant extract clear solution by using a measuring cylinder, pouring into the beaker, adding 200ml of distilled water, and stirring;
(5) weighing 4g of emulsifier and 2g of glycerol, weighing 10ml of dipropylene glycol, pouring into a beaker together, and stirring for 5 min;
(6) dripping 100uL PCA-Na and 10uL essence, and stirring for 5 min;
(7) and (6) packaging.
Example 4
The preparation method of the face cream comprises the following steps:
(1) accurately weighing 3g of pyracantha fortuneana extract and 3g of Convolvulus floridus extract, and putting into a beaker;
(2) weighing 100ml of distilled water, pouring into a beaker, adding a magnet, and heating and dissolving for 1.5 hours in a magnetic stirrer;
(3) after the heating and dissolving are finished, carrying out suction filtration once, and then carrying out filtration once to obtain a plant extract clarified liquid;
(4) taking another clean beaker, weighing 20ml of plant extract clear solution by using a measuring cylinder, pouring into the beaker, adding 200ml of distilled water, and stirring;
(5) weighing 4g of emulsifier and 2g of glycerol, weighing 10ml of dipropylene glycol, pouring into a beaker together, and stirring for 5 min;
(6) dripping 100uL PCA-Na and 10uL essence, and stirring for 5 min;
(7) and (6) packaging.
Claims (1)
1. The application of a plant extract in preparing cosmetics for inhibiting tyrosinase activity is characterized in that the plant extract is a pyracantha fortuneana extract and a bindweed extract according to the weight ratio of 1:1 in a mass ratio;
the extraction method of the pyracantha fortuneana extract comprises the following steps: pulverizing dried fructus Pyracanthae, sieving, and making into powder of 40 mesh; preparing an extracting solution by using water and ethanol according to the volume ratio of 3: 6; accurately weighing pyracantha fortuneana powder in grams, taking an extracting solution in milliliters, taking a solid-to-liquid ratio in grams/milliliters as 1:8, and soaking the pyracantha fortuneana powder in the extracting solution for 48 hours; reflux extracting for 1 hr, stopping extraction, cooling to room temperature, heating for 1 time, and extracting at a temperature not higher than 80 deg.C; cooling, vacuum filtering, and evaporating the solvent from the filtrate with a rotary evaporator to obtain pyracantha fortuneana extract;
the extraction method of the Convolvulus arvensis extract comprises the following steps: selecting dried stem and leaf of Convolvulus, pulverizing, sieving with 100 mesh sieve, adding 6 times of 70% ethanol, reflux extracting for 3 times, mixing filtrates, distilling under reduced pressure to recover ethanol, subjecting the rest filtrate to macroporous adsorbent resin column chromatography, collecting 75% ethanol eluate, and drying under reduced pressure.
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| CN111388367B (en) * | 2020-03-25 | 2021-09-24 | 江南大学 | Composition for inhibiting melanin at multiple target points, preparation method and application of composition in cosmetics |
| CN113476356A (en) * | 2021-04-30 | 2021-10-08 | 云南英格生物技术有限公司 | Preparation method and application of pyracantha fortuneana fruit extract |
| CN113318061A (en) * | 2021-06-16 | 2021-08-31 | 上海南滨江细胞生物科技有限公司 | A facial cream containing embryo extract and its preparation method |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH07238011A (en) * | 1994-02-24 | 1995-09-12 | Suntory Ltd | Skin-beautifying cosmetic composition |
| CN103251659A (en) * | 2013-06-03 | 2013-08-21 | 青岛三九九洲生物技术有限公司 | Preparation method of ginkgo leaf essence |
| CN105434201A (en) * | 2014-09-25 | 2016-03-30 | 伽蓝（集团）股份有限公司 | Composition containing pomegranate extract and use thereof, and skin externally-applied agent |
| CN107384069A (en) * | 2017-06-30 | 2017-11-24 | 天长市巨龙车船涂料有限公司 | A kind of waterborne UV coating |
| CN109125150A (en) * | 2017-06-15 | 2019-01-04 | 新乡医学院三全学院 | A kind of skin care item and the preparation method and application thereof containing hawthorne leaf P.E |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2011504498A (en) * | 2007-11-19 | 2011-02-10 | スティーフェル ラボラトリーズ インコーポレイテッド | Topical whitening cosmetic composition and method of use |
-
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- 2019-08-16 CN CN201910759872.9A patent/CN110433114B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH07238011A (en) * | 1994-02-24 | 1995-09-12 | Suntory Ltd | Skin-beautifying cosmetic composition |
| CN103251659A (en) * | 2013-06-03 | 2013-08-21 | 青岛三九九洲生物技术有限公司 | Preparation method of ginkgo leaf essence |
| CN105434201A (en) * | 2014-09-25 | 2016-03-30 | 伽蓝（集团）股份有限公司 | Composition containing pomegranate extract and use thereof, and skin externally-applied agent |
| CN109125150A (en) * | 2017-06-15 | 2019-01-04 | 新乡医学院三全学院 | A kind of skin care item and the preparation method and application thereof containing hawthorne leaf P.E |
| CN107384069A (en) * | 2017-06-30 | 2017-11-24 | 天长市巨龙车船涂料有限公司 | A kind of waterborne UV coating |
Non-Patent Citations (2)
| Title |
|---|
| 何蓉蓉等.火棘果实提取物的美白作用.《中国实验方剂学杂志》.2011,第17卷(第02期),第184页摘要及第185页第1.1项. * |
| 火棘果实提取物的美白作用;何蓉蓉等;《中国实验方剂学杂志》;20110120;第17卷(第02期);第184页摘要及第185页第1.1项 * |
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