CN113316988B - Method for promoting germination of magnolia multiflora seeds - Google Patents
Method for promoting germination of magnolia multiflora seeds Download PDFInfo
- Publication number
- CN113316988B CN113316988B CN202110598430.8A CN202110598430A CN113316988B CN 113316988 B CN113316988 B CN 113316988B CN 202110598430 A CN202110598430 A CN 202110598430A CN 113316988 B CN113316988 B CN 113316988B
- Authority
- CN
- China
- Prior art keywords
- seeds
- germination
- magnolia
- variegata
- seed
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Soil Sciences (AREA)
- Environmental Sciences (AREA)
- Pretreatment Of Seeds And Plants (AREA)
Abstract
The invention discloses a method for promoting germination of magnolia multiflora bunge seeds, which is to soak the magnolia multiflora bunge seeds for 8-12 hours at the temperature of 65 ℃ by using a green ratio multi-solution with the concentration of 6-9 ml/L. According to the method, the magnolia variegata seeds are soaked in the green-to-multi ratio solution with a specific concentration in a specific time, so that the germination rate, the germination vigor, the germination index and the vitality index of the magnolia variegata seeds can be obviously improved, and the germination performance of the magnolia variegata seeds is obviously improved.
Description
Technical Field
The invention relates to the technical field of magnolia variegata seed treatment, in particular to a method for promoting the germination of magnolia variegata seeds.
Background
Indigofera tinctoria (Indigofera amblyyantha) is perennial shrub of the genus Indigofera of the family Leguminosae (Leguminosae sp.). The root system of the flowery knotweed can effectively intercept water drops and prevent soil from scouring, and the flowery knotweed is a water and soil conservation plant with strong resistance. The root nodule of the magnolia liliiflora can fix nitrogen, so that the nitrogen content of the soil is increased to improve the soil, and the magnolia liliiflora can grow in the soil nutrient-deficient area. Therefore, the flowery knotweed herb is usually used as a pioneer recovery species and a water and soil conservation plant for ecological restoration, has the functions of water and soil conservation, greening, soil quality improvement and the like, and has low germination rate. The reason for the low germination rate of the magnolia liliiflora is related to the seed hard-seed rate and the seed dormancy, the seed dormancy needs to be broken if the germination rate is improved and the seedling emergence is ensured to be uniform, the hard shell of the seed is broken by traditional common sulfuric acid and other medicaments, so that the seed coat absorbs water and breathes air, the seed germination is promoted, and the growth of the germinated radicle and the embryo has certain influence.
Disclosure of Invention
In view of the prior art, the invention aims to provide a method for promoting germination of magnolia variegata seeds, and researches show that the germination rate, the germination vigor, the germination index and the vitality index of the magnolia variegata seeds can be obviously improved by soaking the magnolia variegata seeds in a green-to-green ratio solution with a specific concentration for a specific time, and the germination performance of the magnolia variegata seeds is obviously improved; the green ratio is mainly that natural animal protein is utilized, and biological activation factors such as small molecular active peptide and the like are obtained after hydrolysis, so that the air permeability and the water permeability of seed coats are improved, the life vitality of plants is improved, the metabolism is regulated, the growth potential of the plants is activated, and the germination and the growth are promoted.
In order to achieve the purpose, the invention adopts the following technical scheme:
according to the first aspect of the invention, the method for promoting the germination of the magnolia variegata seeds is to soak the magnolia variegata seeds in the green ratio multi-solution with the concentration of 6-9ml/L for 8-12 hours at the temperature of 60-65 ℃.
Preferably, the method is to soak the seed of the magnolia variegata by using a green ratio multiple solution with the concentration of 6ml/L and the temperature of 65 ℃ for 12 hours.
Preferably, the LVBIODU solution is purchased from Zhan China investment Co.
In a second aspect of the present invention, there is provided a magnolia biondii seed prepared by the above method.
In a third aspect of the invention, the use of the above method for promoting germination of magnolia variegata seeds is provided.
Preferably, germination of the magnolia biondii seeds is promoted by at least one of the following pathways (1) to (3):
(1) the germination rate of the magnolia variegata seeds is improved;
(2) the germination vigor of the magnolia variegata seeds is improved;
(3) improving the germination index of the magnolia variegata seeds;
(4) improving the vitality index of the magnolia variegata seeds.
The invention has the beneficial effects that:
the method has the advantages that the magnolia variegata seeds are soaked in the green ratio multi-solution with specific concentration at specific temperature and time, so that the germination rate, the germination vigor, the germination index and the vitality index of the magnolia variegata seeds can be obviously improved, and the germination performance of the magnolia variegata seeds is obviously improved.
Detailed Description
It should be noted that the following detailed description is exemplary and is intended to provide further explanation of the disclosure. Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this application belongs.
As described in the background section, the flowery knotweed is generally used as a pioneer recovery species and a water and soil conservation plant for ecological restoration, and has the functions of water and soil conservation, greening, soil quality improvement and the like, but the flowery knotweed has low germination rate. The reason for the low germination rate of the magnolia variegata is related to the seed hard-seed rate and the seed dormancy, and the dormancy of the seeds must be broken to improve the germination rate and ensure the uniformity of the seedling emergence, so that the hard shells of the seeds are broken, the seed coats absorb water and ventilate, and the seed germination is promoted.
According to the invention, through research, the method can obviously improve the germination rate, the germination vigor, the germination index and the vigor index of the magnolia biondii seeds and obviously improve the germination performance of the magnolia biondii seeds by soaking the magnolia biondii seeds in the green ratio multi-solution with the concentration of 6ml/L and the temperature of 65 ℃ for 12 hours.
In order to make the technical solutions of the present application more clearly understood by those skilled in the art, the technical solutions of the present application will be described in detail below with reference to specific embodiments.
The test materials used in the examples of the present invention are all conventional in the art and commercially available.
Example 1: a method for promoting germination of the seed of the magnolia variegata is to soak the seed of the magnolia variegata for 12 hours at a temperature of 65 ℃ by using a green ratio multi-solution with a concentration of 6 ml/L. The multiple green ratio solution is a Xiongdaoda multiple green ratio solution, and the original country: italy, the linkage in china: just reaching the investment company of China.
1. Germination test in culture dish under phytotron conditions
(1) Seed disinfection: sterilizing the seeds in 5% sodium hypochlorite solution for 5-8min, pouring out sodium hypochlorite, and washing with sterilized water for 3 times;
(2) seed soaking: averagely dividing the same batch of the kalanchoe odorata seeds into 16 groups, respectively soaking the seeds by green multi-ratio solutions of 0ml/L, 3ml/L, 6ml/L and 9ml/L, and respectively dividing the green multi-ratio solutions at each concentration into 4 groups according to seed soaking time, wherein the seed soaking time is 4 hours, 8 hours, 12 hours and 24 hours;
(3) and (3) germination: the seeds after seed soaking were placed in a petri dish for germination in a 25 ℃ culture room, the number of germinated seeds was recorded every day, and the germination vigor was measured at 3 d.
(4) Determining the germination rate, the germination vigor, the germination index and the vitality index of the seed germination;
the germination rate is (number of germinated seeds at the final germination stage/total number of tested seeds) × 100%;
germination potential (number of seeds germinated on 3 d/total number of seeds tested) × 100%;
germination index ═ Σ (Gt/Dt);
the vitality index is multiplied by the germination index multiplied by G;
in the above formula: gt is the number of sprouts in t days; dt is the corresponding number of germination days; g is the weight of the seedling at the end of germination. The results are shown in tables 1 to 4.
TABLE 1 Effect of different greenness ratios and seed soaking time on the germination percentage of the seeds of Magnolia liliflora (%)
TABLE 2 Effect of different greenness ratios and seed soaking time on the germination vigor of the seeds of Ficus carica (%)
TABLE 3 Effect of different greenness ratios and seed soaking times on germination index (%)
TABLE 4 Effect of different Green ratio concentrations and seed soaking time on the viability index of the Magnolia liliflora seeds
As can be seen from tables 1 to 4, the germination rate, germination vigor, germination index and vigor index of the magnolia biondii seeds obtained by soaking the seeds in the green ratio multi-solution with the concentration of 6ml/L for 12 hours are optimal.
(3) In order to determine the influence of the seed soaking of the multiple solutions with different green ratios on the germination of the magnolia liliflora seeds, the seed soaking solution with the optimal concentration of 6ml/L is set to be at the temperature of 25 ℃, 40 ℃, 50 ℃, 60 ℃, 65 ℃ and 70 ℃ for soaking the seeds for 12 hours, the seeds are germinated under the temperature of 25 ℃, and then the germination rate, the germination vigor, the germination index and the vitality index of the seed germination are measured, and the measurement results are shown in table 5.
TABLE 5 Effect of different temperature Green ratio solution seed soaking on the Germination of Murraya floribunda Linn seeds
As can be seen from Table 5, increasing the temperature of the seed-soaking solution increased the germination performance of the magnolia biondii seeds, with 65 ℃ being the optimum temperature.
2. Testing in field environment
The test is carried out in scientific research test base of the university of Hunan agriculture, the university of Hunan, the period from 5 months to 6 months in 2020.
(1) Seed disinfection: sterilizing the seeds in 5% sodium hypochlorite solution for 5-8min, pouring out sodium hypochlorite, and washing with sterilized water for 3 times;
(2) seed soaking: averagely dividing the same batch of the magnolia variegata seeds into two groups, and soaking the seeds for 12 hours at 65 ℃ through 0ml/L and 6ml/L green multi-ratio solutions respectively;
(3) sowing: sowing the soaked seed of the magnolia variegata in a ploughed and raked terrace bed, and covering soil on the surface of the sowed seed by about 0.5 cm;
(4) the germination rate, germination vigor, germination index and vitality index of the seed germination were measured, and the measurement results are shown in table 6.
TABLE 6 influence of multiple seed soaking in Green ratio on the germination of Murraya floribunda seeds
Therefore, the germination performance of the magnolia variegata seeds under the field environment condition can be obviously improved by soaking the magnolia variegata seeds obtained by the magnolia variegata solution with the concentration of 6ml/L for 12 hours at the temperature of 65 ℃.
The above description is only a preferred embodiment of the present application and is not intended to limit the present application, and various modifications and changes may be made by those skilled in the art. Any modification, equivalent replacement, improvement and the like made within the spirit and principle of the present application shall be included in the protection scope of the present application.
Claims (2)
1. A method for promoting the germination of the magnolia variegata seeds is characterized in that: the method is to soak the seed of the magnolia variegata at the temperature of 65 ℃ for 12 hours by using a green ratio multi-solution with the concentration of 6 ml/L.
2. Use of the method of claim 1 in at least one of the following (1) to (4):
(1) the germination rate of the magnolia variegata seeds is improved;
(2) the germination vigor of the magnolia variegata seeds is improved;
(3) improving the germination index of the magnolia variegata seeds;
(4) improving the vitality index of the magnolia variegata seeds.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110598430.8A CN113316988B (en) | 2021-05-31 | 2021-05-31 | Method for promoting germination of magnolia multiflora seeds |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110598430.8A CN113316988B (en) | 2021-05-31 | 2021-05-31 | Method for promoting germination of magnolia multiflora seeds |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN113316988A CN113316988A (en) | 2021-08-31 |
| CN113316988B true CN113316988B (en) | 2022-04-19 |
Family
ID=77422570
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202110598430.8A Active CN113316988B (en) | 2021-05-31 | 2021-05-31 | Method for promoting germination of magnolia multiflora seeds |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN113316988B (en) |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103141177A (en) * | 2013-02-24 | 2013-06-12 | 康建国 | Hard seed processing method |
| CN109422587A (en) * | 2017-08-28 | 2019-03-05 | 邹亚燕 | A kind of amino acid fertilizer and its preparation method and application |
| CN110278736A (en) * | 2019-07-09 | 2019-09-27 | 中山市中茂环境建设有限公司 | A kind of Chinese photinia seed sprouting technique |
-
2021
- 2021-05-31 CN CN202110598430.8A patent/CN113316988B/en active Active
Also Published As
| Publication number | Publication date |
|---|---|
| CN113316988A (en) | 2021-08-31 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN111201956B (en) | Application of melatonin in purple perilla cultivation | |
| CN116478870A (en) | Maltophilous oligotrophic single spore fungus OLR3-17 strain and application thereof | |
| CN112470929B (en) | Method for obtaining regeneration plant from root-neck apical tissue of rhodiola crenulata | |
| CN112020930B (en) | Method for inducing rice to improve salt tolerance | |
| CN110604049B (en) | Wild-returning ecological planting method for dendrobium officinale | |
| CN113316988B (en) | Method for promoting germination of magnolia multiflora seeds | |
| CN108770865B (en) | Alba elymus nutans seed coating agent | |
| KR20090087553A (en) | Germinating method for camptotheca acuminate | |
| CN107548848B (en) | Antibacterial disease-preventing synergistic seedling-protecting method for seedling-culturing period of seedlings of fragrant fruit trees | |
| CN113598055B (en) | Ainsliaea fragrans champ germplasm improvement method | |
| CN113349227B (en) | Tobacco seed germination-promoting stress-resistant seedling-strengthening composition, preparation and use method | |
| CN110771507B (en) | Preparation method of artificial astragalus membranaceus seeds | |
| Li et al. | Methods for breaking the dormancy of eastern redbud (Cercis canadensis) seeds | |
| CN114793828A (en) | Tobacco seedling culture medium and preparation and use methods thereof | |
| CN110771510B (en) | Method for preparing artificial clove seeds | |
| CN107409896A (en) | A kind of seeding growing seedlings method of golden yellow panda tree | |
| CN109601058B (en) | Method for cultivating strong seedlings of delayed-cultivation watermelons in autumn | |
| CN113892425A (en) | Rapid cultivation method of hazelnut semi-aseptic seedlings | |
| CN108633379B (en) | Method for breaking dormancy of cercis gigantea seeds | |
| CN111528090A (en) | Strawberry tissue culture seedling hardening matrix for improving hardening quality of strawberry tissue culture seedlings and preparation and use methods and application thereof | |
| CN111837961A (en) | Dendrobium officinale seedling culture medium and seedling culture method | |
| CN110881321A (en) | Cutting propagation method for upright stems of rhizoma phragmitis of amomum villosum | |
| CN110558216A (en) | Eustoma russellianum seedling growing method for eliminating rosette characters through temperature and light regulation | |
| CN110268941A (en) | A kind of pseudo-ginseng plains region cultural method | |
| CN108966740A (en) | A method of promote to germinate under cotton low temperature stress |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |