CN113293189A - Extraction method and application of safflower polypeptide - Google Patents
Extraction method and application of safflower polypeptide Download PDFInfo
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- CN113293189A CN113293189A CN202110628796.5A CN202110628796A CN113293189A CN 113293189 A CN113293189 A CN 113293189A CN 202110628796 A CN202110628796 A CN 202110628796A CN 113293189 A CN113293189 A CN 113293189A
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- 235000003255 Carthamus tinctorius Nutrition 0.000 title claims abstract description 91
- 244000020518 Carthamus tinctorius Species 0.000 title claims abstract description 91
- 108090000765 processed proteins & peptides Proteins 0.000 title claims abstract description 79
- 102000004196 processed proteins & peptides Human genes 0.000 title claims abstract description 69
- 229920001184 polypeptide Polymers 0.000 title claims abstract description 67
- 238000000605 extraction Methods 0.000 title claims abstract description 18
- 239000000843 powder Substances 0.000 claims abstract description 18
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 claims abstract description 16
- 102000004169 proteins and genes Human genes 0.000 claims abstract description 16
- 108090000623 proteins and genes Proteins 0.000 claims abstract description 16
- 238000000034 method Methods 0.000 claims abstract description 15
- 239000003480 eluent Substances 0.000 claims abstract description 13
- 108091005804 Peptidases Proteins 0.000 claims abstract description 11
- 239000004365 Protease Substances 0.000 claims abstract description 11
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 claims abstract description 11
- 239000006228 supernatant Substances 0.000 claims abstract description 11
- 239000012528 membrane Substances 0.000 claims abstract description 10
- 102000004190 Enzymes Human genes 0.000 claims abstract description 8
- 108090000790 Enzymes Proteins 0.000 claims abstract description 8
- 238000001914 filtration Methods 0.000 claims abstract description 8
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims abstract description 6
- 238000001816 cooling Methods 0.000 claims abstract description 6
- 239000008103 glucose Substances 0.000 claims abstract description 6
- 238000000227 grinding Methods 0.000 claims abstract description 6
- 238000002386 leaching Methods 0.000 claims abstract description 6
- 239000000126 substance Substances 0.000 claims abstract description 6
- 241000628997 Flos Species 0.000 claims abstract description 3
- 239000000243 solution Substances 0.000 claims description 15
- 239000007788 liquid Substances 0.000 claims description 9
- 108010007119 flavourzyme Proteins 0.000 claims description 7
- 238000009835 boiling Methods 0.000 claims description 6
- 239000000047 product Substances 0.000 claims description 6
- 229910019142 PO4 Inorganic materials 0.000 claims description 5
- 239000007864 aqueous solution Substances 0.000 claims description 5
- 239000003814 drug Substances 0.000 claims description 5
- 230000036541 health Effects 0.000 claims description 5
- 239000010452 phosphate Substances 0.000 claims description 5
- 238000000108 ultra-filtration Methods 0.000 claims description 5
- 238000009777 vacuum freeze-drying Methods 0.000 claims description 5
- 108090000145 Bacillolysin Proteins 0.000 claims description 4
- 108091005658 Basic proteases Proteins 0.000 claims description 4
- 108091005507 Neutral proteases Proteins 0.000 claims description 4
- 102000035092 Neutral proteases Human genes 0.000 claims description 4
- 239000002537 cosmetic Substances 0.000 claims description 3
- 235000013305 food Nutrition 0.000 claims description 3
- 229940079593 drug Drugs 0.000 claims description 2
- 125000002467 phosphate group Chemical group [H]OP(=O)(O[H])O[*] 0.000 claims description 2
- 230000002255 enzymatic effect Effects 0.000 claims 1
- 238000010521 absorption reaction Methods 0.000 abstract description 11
- 230000000694 effects Effects 0.000 abstract description 6
- 150000004676 glycans Chemical class 0.000 abstract description 4
- 229920001282 polysaccharide Polymers 0.000 abstract description 4
- 239000005017 polysaccharide Substances 0.000 abstract description 4
- 238000005516 engineering process Methods 0.000 abstract description 3
- 230000000415 inactivating effect Effects 0.000 abstract 1
- 235000001014 amino acid Nutrition 0.000 description 14
- 150000001413 amino acids Chemical class 0.000 description 14
- 235000018102 proteins Nutrition 0.000 description 13
- 210000003491 skin Anatomy 0.000 description 7
- 230000017531 blood circulation Effects 0.000 description 6
- 230000032823 cell division Effects 0.000 description 4
- 239000008280 blood Substances 0.000 description 3
- 210000004369 blood Anatomy 0.000 description 3
- 238000004140 cleaning Methods 0.000 description 3
- 238000001035 drying Methods 0.000 description 3
- 235000015097 nutrients Nutrition 0.000 description 3
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 3
- 230000001737 promoting effect Effects 0.000 description 3
- 230000008439 repair process Effects 0.000 description 3
- 238000000926 separation method Methods 0.000 description 3
- 235000015655 Crocus sativus Nutrition 0.000 description 2
- 244000124209 Crocus sativus Species 0.000 description 2
- 230000003712 anti-aging effect Effects 0.000 description 2
- 210000004027 cell Anatomy 0.000 description 2
- 230000024245 cell differentiation Effects 0.000 description 2
- 150000001875 compounds Chemical class 0.000 description 2
- 230000001276 controlling effect Effects 0.000 description 2
- 210000001198 duodenum Anatomy 0.000 description 2
- 238000005265 energy consumption Methods 0.000 description 2
- 230000035876 healing Effects 0.000 description 2
- 235000016709 nutrition Nutrition 0.000 description 2
- 230000035764 nutrition Effects 0.000 description 2
- 230000019612 pigmentation Effects 0.000 description 2
- 238000001243 protein synthesis Methods 0.000 description 2
- 230000008929 regeneration Effects 0.000 description 2
- 238000011069 regeneration method Methods 0.000 description 2
- 230000001105 regulatory effect Effects 0.000 description 2
- 230000014616 translation Effects 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- 208000019901 Anxiety disease Diseases 0.000 description 1
- 241000208838 Asteraceae Species 0.000 description 1
- 241000208809 Carthamus Species 0.000 description 1
- 208000032544 Cicatrix Diseases 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 102000010834 Extracellular Matrix Proteins Human genes 0.000 description 1
- 108010037362 Extracellular Matrix Proteins Proteins 0.000 description 1
- 206010021143 Hypoxia Diseases 0.000 description 1
- 241001113425 Iridaceae Species 0.000 description 1
- 206010072170 Skin wound Diseases 0.000 description 1
- 150000001371 alpha-amino acids Chemical class 0.000 description 1
- 235000008206 alpha-amino acids Nutrition 0.000 description 1
- 230000036506 anxiety Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 235000019658 bitter taste Nutrition 0.000 description 1
- 210000004204 blood vessel Anatomy 0.000 description 1
- 230000001684 chronic effect Effects 0.000 description 1
- 238000009833 condensation Methods 0.000 description 1
- 230000005494 condensation Effects 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 230000018044 dehydration Effects 0.000 description 1
- 238000006297 dehydration reaction Methods 0.000 description 1
- 210000004207 dermis Anatomy 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 230000004069 differentiation Effects 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 210000001339 epidermal cell Anatomy 0.000 description 1
- 210000002615 epidermis Anatomy 0.000 description 1
- 210000002744 extracellular matrix Anatomy 0.000 description 1
- 210000002950 fibroblast Anatomy 0.000 description 1
- 239000003205 fragrance Substances 0.000 description 1
- 230000007062 hydrolysis Effects 0.000 description 1
- 238000006460 hydrolysis reaction Methods 0.000 description 1
- 239000013067 intermediate product Substances 0.000 description 1
- 239000002932 luster Substances 0.000 description 1
- 239000008176 lyophilized powder Substances 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 229930014626 natural product Natural products 0.000 description 1
- 230000017854 proteolysis Effects 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 231100000241 scar Toxicity 0.000 description 1
- 230000037387 scars Effects 0.000 description 1
- 230000003860 sleep quality Effects 0.000 description 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/06—Preparation of peptides or proteins produced by the hydrolysis of a peptide bond, e.g. hydrolysate products
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/145—Extraction; Separation; Purification by extraction or solubilisation
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/16—Extraction; Separation; Purification by chromatography
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/34—Extraction; Separation; Purification by filtration, ultrafiltration or reverse osmosis
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/36—Extraction; Separation; Purification by a combination of two or more processes of different types
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- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Molecular Biology (AREA)
- Analytical Chemistry (AREA)
- Biophysics (AREA)
- Medicinal Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Water Supply & Treatment (AREA)
- Biotechnology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Microbiology (AREA)
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- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
Abstract
The invention discloses a safflower polypeptide extraction method and application, the method comprises the following steps: grinding safflower seeds to obtain safflower powder, adding NaOH solution into the safflower powder, leaching for 1-1.5h, centrifuging to remove insoluble substances to obtain safflower protein extract, adjusting pH to 6-7, and adding enzymolysis protease at 45-60 deg.C for enzymolysis to obtain enzymolysis solution; inactivating enzyme, cooling, centrifuging, collecting supernatant, ultrafiltering with filter membrane, separating with glucose gel column, eluting with eluent, collecting eluate, and filtering to obtain Carthami flos polypeptide extract. The extraction method of the safflower polypeptide provided by the invention adopts a biological enzymolysis technology, successfully converts the safflower polysaccharide into active small molecular peptides, can be actively digested and utilized by human bodies, and has high absorption rate; the safflower polypeptide extracted by the safflower polypeptide extraction method provided by the invention is lower than 500 daltons, the purity is high, the method is simple to operate, the extraction condition is mild, and the obtained polypeptide can keep the natural activity of the polypeptide.
Description
Technical Field
The invention relates to the technical field of natural product extraction, and particularly relates to a safflower polypeptide extraction method and application.
Background
Modern women bear great working and living pressure, are easy to cause anxiety, uneasiness, sleep quality reduction and blood viscosity increase, lead capillary vessels invisible to the naked eye to be silted up, lead the blood flow speed to be slow and lead the skin to be anoxic for tens of millions. In severe cases, pigmentation is caused by blood stasis, water is extruded out of blood vessels, and further, the skin loses natural luster due to oxygen deficiency, and pigmentation spots appear on the surface of the skin. The skin care product containing polypeptide can stimulate the differentiation and the regeneration of epidermal cells of skin, strongly promote the repair and the healing of the epidermis and inhibit the formation of scars. The skin care product containing the polypeptide can provide nutrients for skin dermis, stimulate the activity of fibroblast, promote the healing and function regeneration of skin wounds and strengthen extracellular matrix structures.
Safflower, also known as "Reddish blue flower" or "Daihong" flower, dicotyledonous plant, Compositae, Carthamus plant, dried tubular flower is about 1.5 cm long, orange red, yellow in anther, combined into tube, higher than splinters, with special fragrance and slightly bitter taste. The preferred one is long, bright red, soft. The safflower is not only required to be taken by sick people for treating diseases, but also is a health-care drink for healthy and sub-healthy people. For example, chronic patients can be used for promoting blood circulation and removing blood stasis, women can be used for promoting blood circulation and caring skin, and the elderly can be used for dredging channels and promoting blood circulation. However, no safflower polypeptide extraction method is reported at present.
Disclosure of Invention
Aiming at the technical problems in the related art, the invention provides a safflower polypeptide extraction method and application, which can overcome the defects in the prior art.
In order to achieve the technical purpose, the technical scheme of the invention is realized as follows:
a method for extracting safflower polypeptide comprises the following steps:
s1 grinding the washed and dried safflower seeds to obtain safflower powder, adding Na0H solution into the safflower powder until the pH value is 8.5-9, leaching for 1-1.5h at 35-45 ℃, and then centrifuging to remove insoluble substances to obtain safflower protein extract;
s2, adjusting the pH value of the obtained safflower protein extract to 6-7, and adding enzymolysis protease at 45-60 ℃ for enzymolysis to obtain an enzymolysis liquid;
s3 boiling the obtained enzymolysis liquid to inactivate enzyme, cooling and centrifuging to obtain supernatant containing Carthami flos polypeptide;
s4 ultrafiltering the obtained supernatant containing safflower polypeptide with filter membrane, separating with glucose gel column, eluting with eluent, collecting eluent, and filtering to obtain safflower polypeptide extractive solution.
Further, the method for extracting safflower polypeptide also comprises:
s5 vacuum freeze-drying the obtained safflower polypeptide extract to obtain the safflower polypeptide extract freeze-dried powder.
Further, the enzymolysis protease is one or more of alkaline protease, flavourzyme and neutral protease.
Further, the filtration membrane is a 500Da ultrafiltration membrane.
Further, the enzyme is boiled for 15-20 min.
Further, the eluent is a phosphate aqueous solution.
According to another aspect of the present invention, there is provided the use of the safflower polypeptide obtained by the said extraction method in food, cosmetics, health products and pharmaceuticals.
The invention has the beneficial effects that: the extraction method of the safflower polypeptide provided by the invention adopts a biological enzymolysis technology to successfully convert the safflower polysaccharide into the active safflower polypeptide, the average molecular weight is less than 1000 daltons, the proportion of various nutrient components is realized, the extraction method is superior to the absorption of amino acid or protein, the safflower polypeptide can be actively digested and utilized by human bodies, and the absorption rate is high; the safflower polypeptide extracted by the safflower polypeptide extraction method provided by the invention is lower than 500 daltons, the purity is high, the method is simple to operate, the extraction condition is mild, the obtained polypeptide can keep the natural activity of the polypeptide, can ensure the normal cell division and the normal protein synthesis by regulating and controlling the vital activity of cells, plays an important role in cell division, differentiation and repair, and has close correlation and great significance with human health, anti-aging, life prolonging and the like; compared with amino acid, the safflower polypeptide extracted by the invention has the following advantages as small molecular protein peptide: firstly, the absorption is faster than that of amino acid; secondly, the medicine is absorbed by the body in an integral form; thirdly, active absorption (amino acid is passively absorbed); fourthly, the consumption is low, compared with amino acid, the peptide absorption has the characteristic of low consumption or no energy consumption, and the peptide directly enters blood circulation after being absorbed by duodenum and conveys self energy nutrition to each part of human body; fifthly, the peptide absorbs amino acid and has the characteristic of unsaturation.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all embodiments. All other embodiments that can be derived by one of ordinary skill in the art from the embodiments given herein are intended to be within the scope of the present invention.
Example 1
A method for extracting safflower polypeptide, which comprises the following steps:
s1 cleaning and drying the safflower seed kernels, grinding to obtain safflower powder, adding NaOH solution into the safflower powder until the pH value is 8.5, leaching for 1.5h at the temperature of 35 ℃, and centrifuging to remove insoluble substances to obtain a safflower protein extracting solution;
s2, adjusting the pH value of the safflower protein extract obtained in the step S1 to 7, adding enzymolysis protease at the temperature of 45 ℃ for enzymolysis to obtain enzymolysis liquid, wherein the enzymolysis protease is alkaline protease and flavourzyme, and the mass ratio of the alkaline protease to the flavourzyme is 1: 1;
s3, boiling the enzymolysis liquid obtained in the step S2 for 15min to inactivate enzyme, cooling and centrifuging to obtain supernatant containing safflower polypeptide;
s4, ultrafiltering the supernatant containing safflower polypeptide obtained in the step S3 by using a 500Da ultrafiltration membrane, then carrying out glucose gel column separation, eluting by using a phosphate aqueous solution as an eluent, collecting the eluent, and filtering to obtain a safflower polypeptide extracting solution.
S5, carrying out vacuum freeze-drying on the safflower polypeptide extract obtained in the step S4 to obtain the safflower polypeptide extract freeze-dried powder.
Example 2
S1 cleaning and drying the safflower seed kernels, grinding to obtain safflower powder, adding NaOH solution into the safflower powder until the pH value is 8.5, leaching for 1.5h at the temperature of 40 ℃, and centrifuging to remove insoluble substances to obtain a safflower protein extracting solution;
s2, adjusting the pH value of the safflower protein extract obtained in the step S1 to 6.5, and adding enzymolysis protease at 45 ℃ for enzymolysis to obtain an enzymolysis solution, wherein the enzymolysis protease is neutral protease and flavourzyme, and the mass ratio of the neutral protease to the flavourzyme is 1: 1;
s3, boiling the enzymolysis liquid obtained in the step S2 for 18min to inactivate enzyme, cooling and centrifuging to obtain supernatant containing safflower polypeptide;
s4, ultrafiltering the supernatant containing safflower polypeptide obtained in the step S3 by using a 500Da ultrafiltration membrane, then carrying out glucose gel column separation, eluting by using a phosphate aqueous solution as an eluent, collecting the eluent, and filtering to obtain a safflower polypeptide extracting solution.
S5, carrying out vacuum freeze-drying on the safflower polypeptide extract obtained in the step S4 to obtain the safflower polypeptide extract freeze-dried powder.
Example 3
A method for extracting safflower polypeptide, which comprises the following steps:
s1, cleaning and drying safflower seeds, grinding to obtain safflower powder, adding a NaOH solution into the safflower powder until the pH value is 9, leaching for 1h at the temperature of 45 ℃, and centrifuging to remove insoluble substances to obtain a safflower protein extracting solution;
s2, adjusting the pH value of the safflower protein extract obtained in the step S1 to 6, and adding enzymolysis protease at the temperature of 60 ℃ for enzymolysis to obtain enzymolysis liquid, wherein the enzymolysis protease is flavourzyme;
s3, boiling the enzymolysis liquid obtained in the step S2 for 20min to inactivate enzyme, cooling, centrifuging, and collecting supernatant containing safflower polypeptide;
s4, ultrafiltering the supernatant containing safflower polypeptide obtained in the step S3 by using a 500Da ultrafiltration membrane, then carrying out glucose gel column separation, eluting by using a phosphate aqueous solution as an eluent, collecting the eluent, and filtering to obtain a safflower polypeptide extracting solution.
S5, carrying out vacuum freeze-drying on the safflower polypeptide extract obtained in the step S4 to obtain the safflower polypeptide extract freeze-dried powder.
The lyophilized powder of safflower polypeptide extract (safflower peptide) obtained in examples 1 to 3 was in the form of powder having a molecular weight of < 500Da, and the extract was derived from dried stigma of Crocus sativus L (Crocus sativus L) belonging to the family Iridaceae, and the safflower polypeptide was a compound in which α -amino acids were linked together by peptide chain, and it was also an intermediate product of proteolysis. A compound obtained by dehydration condensation of a 10-100 amino acid molecule is generally called a polypeptide. The detection method is a hydrolysis method, the total number of colonies is less than 1000, the solubility is more than or equal to 90 percent, the water content is less than or equal to 6 percent, the product is sealed and shielded from light and high temperature, is stored in a dry, cool and ventilated place, and can be applied to the fields of food, cosmetics, health products, medicines and the like.
In conclusion, by means of the technical scheme, the safflower polysaccharide is successfully converted into active small molecular peptides by adopting a biological enzymolysis technology, the average molecular weight is less than 1000 daltons, the proportion of various nutrient components is realized, the amino acid or protein is better than the absorption of amino acid or protein, the safflower polysaccharide can be actively digested and utilized by a human body, and the absorption rate is high; the safflower polypeptide extracted by the safflower polypeptide extraction method provided by the invention is lower than 500 daltons, the purity is high, the method is simple to operate, the extraction condition is mild, the obtained polypeptide can keep the natural activity of the polypeptide, can ensure the normal cell division and the normal protein synthesis by regulating and controlling the vital activity of cells, plays an important role in cell division, differentiation and repair, and has close correlation and great significance with human health, anti-aging, life prolonging and the like; compared with amino acid, the safflower polypeptide extracted by the invention has the following advantages as small molecular protein peptide: firstly, the absorption is faster than that of amino acid; secondly, the medicine is absorbed by the body in an integral form; thirdly, active absorption (amino acid is passively absorbed); fourthly, the consumption is low, compared with amino acid, the peptide absorption has the characteristic of low consumption or no energy consumption, and the peptide directly enters blood circulation after being absorbed by duodenum and conveys self energy nutrition to each part of human body; fifthly, the peptide absorbs amino acid and has the characteristic of unsaturation.
The above description is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the invention, and any modifications, equivalents, improvements and the like that fall within the spirit and principle of the present invention are intended to be included therein.
Claims (7)
1. The method for extracting the safflower polypeptide is characterized by comprising the following steps:
s1 grinding the washed and dried safflower seeds to obtain safflower powder, adding NaOH solution into the safflower powder until the pH value is 8.5-9, leaching for 1-1.5h at 35-45 ℃, and then centrifuging to remove insoluble substances to obtain safflower protein extract;
s2, adjusting the pH value of the obtained safflower protein extract to 6-7, and adding enzymolysis protease at 45-60 ℃ for enzymolysis to obtain an enzymolysis liquid;
s3 boiling the obtained enzymolysis liquid to inactivate enzyme, cooling and centrifuging to obtain supernatant containing Carthami flos polypeptide;
s4 ultrafiltering the obtained supernatant containing safflower polypeptide with filter membrane, separating with glucose gel column, eluting with eluent, collecting eluent, and filtering to obtain safflower polypeptide extractive solution.
2. The method for extracting safflower polypeptide of claim 1, further comprising:
s5 vacuum freeze-drying the obtained safflower polypeptide extract to obtain the safflower polypeptide extract freeze-dried powder.
3. The method for extracting safflower polypeptide of claim 1, wherein the enzymatic protease is one or more of alkaline protease, flavourzyme and neutral protease.
4. The method for extracting safflower polypeptide of claim 1, wherein the filtration membrane is a 500Da ultrafiltration membrane.
5. The method for extracting safflower polypeptide of claim 1, wherein the time for boiling to inactivate the enzyme is 15-20 min.
6. The method for extracting safflower polypeptide of claim 1, wherein the eluent is a phosphate aqueous solution.
7. Use of the safflower polypeptide obtained by the extraction method according to any one of claims 1 to 6 in foods, cosmetics, health products and medicines.
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| CN202110628796.5A CN113293189A (en) | 2021-06-04 | 2021-06-04 | Extraction method and application of safflower polypeptide |
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| CN202110628796.5A CN113293189A (en) | 2021-06-04 | 2021-06-04 | Extraction method and application of safflower polypeptide |
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104630317A (en) * | 2014-10-13 | 2015-05-20 | 石河子大学 | Method for preparing safflower seed antioxidant peptide |
| CN106433993A (en) * | 2016-12-20 | 2017-02-22 | 乌鲁木齐上善元生物科技有限公司 | Extracting method of unsaturated fatty acid in safflower oil |
| CN107653289A (en) * | 2017-11-02 | 2018-02-02 | 林峰 | A kind of industrialized production kardiseed active peptide and preparation method |
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2021
- 2021-06-04 CN CN202110628796.5A patent/CN113293189A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104630317A (en) * | 2014-10-13 | 2015-05-20 | 石河子大学 | Method for preparing safflower seed antioxidant peptide |
| CN106433993A (en) * | 2016-12-20 | 2017-02-22 | 乌鲁木齐上善元生物科技有限公司 | Extracting method of unsaturated fatty acid in safflower oil |
| CN107653289A (en) * | 2017-11-02 | 2018-02-02 | 林峰 | A kind of industrialized production kardiseed active peptide and preparation method |
Non-Patent Citations (1)
| Title |
|---|
| 孙立: "红花籽粕抗氧化肽的制备及抗氧化活性研究", 《中国优秀硕士学位论文全文数据库（电子期刊）》 * |
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Application publication date: 20210824 |