CN113265438B

CN113265438B - Preparation method of silk antibacterial peptide and application of silk antibacterial peptide in acne-removing cosmetics

Info

Publication number: CN113265438B
Application number: CN202110610177.3A
Authority: CN
Inventors: 盘学平
Original assignee: Guangzhou Shangzi Chemical Technology Co ltd
Current assignee: Guangzhou Shangzi Chemical Technology Co ltd
Priority date: 2021-06-01
Filing date: 2021-06-01
Publication date: 2022-07-29
Anticipated expiration: 2041-06-01
Also published as: CN113265438A

Abstract

The invention discloses a preparation method of silk antibacterial peptide, which comprises the following steps: pretreatment of silkworms: collecting, cleaning and drying silkworms; degumming process: boiling silk in water to obtain degummed fibroin; and (3) a salt dissolving process: adding saturated CaCl ₂ Heating the water solution, stirring the fibroin, and filtering; and (3) membrane separation process: desalting with dialysis bag; and (3) enzymolysis: performing enzymolysis with compound protease, inactivating enzyme, cooling, centrifuging, and collecting supernatant; and (3) decoloring process: decolorizing the sample with charcoal; and (3) freeze drying: freeze drying and pulverizing into powder to obtain the silk antibacterial peptide freeze-dried agent. The invention also discloses a composition which comprises the silk antibacterial peptide freeze-dried powder, arbutin, glycerol, isopropyl myristate and water. Has antibacterial and repairing effects, and can be used for removing pox more easily without recurrence.

Description

Preparation method of silk antibacterial peptide and application of silk antibacterial peptide in acne-removing cosmetics

Technical Field

The invention relates to the field of cosmetics, and relates to silk peptide, a preparation method and a composition thereof, in particular to silk peptide, a preparation method thereof and application of a composition containing the silk peptide in acne-removing cosmetics.

Background

The antibacterial peptide is a kind of antimicrobial and some malignant cell short peptide produced by organism in the defense reaction against pathogenic microorganism. It is an important component of the innate immune system of the body and has a broad inhibitory effect on bacteria, fungi, parasites, viruses, tumor cells and the like.

Acne occurs in close relation to factors such as hyperseborrhea, blockage of pilosebaceous ducts, bacterial infection and inflammatory reactions. After adolescence, the level of androgen, particularly testosterone, in a human body is rapidly increased, the development of sebaceous glands is promoted, and a large amount of sebum is produced. Meanwhile, abnormal keratinization of the pilosebaceous canal causes the canal to be blocked, sebum is obstructed to be discharged, and a keratoplug, namely micro acne, is formed. A number of microorganisms in the hair follicle, particularly propionibacterium acnes, multiply in numbers, and lipases produced by propionibacterium acnes break down sebum to produce free fatty acids, while chemotactic inflammatory cells and mediators, ultimately inducing and exacerbating the inflammatory response.

Disclosure of Invention

In order to solve the problems in the prior art, the first aspect of the present invention provides a method for preparing a silk peptide, comprising the steps of: and (3) carrying out enzymolysis on the crude silk protein by using papain and/or subtilisin to obtain the silk peptide.

In some embodiments, the enzymatic substrate solution is a crude fibroin aqueous solution with a concentration of 4-7% (e.g., 4.5%, 5.0%, 5.5%, 6.0%, 6.5%) by weight.

In some embodiments, the enzymatic substrate solution has a pH of 4.0-8.0 (e.g., 4.5, 5.0, 5.5, 6.0, 6.5, 7.0, 7.5).

In some embodiments, in the reaction system of the enzymatic hydrolysis, the mass ratio of the papain to the crude silk protein is as follows: 0.25-0.75:100 (e.g., 0.30:100, 0.35:100, 0.40:100, 0.45:100, 0.50:100, 0.55:100, 0.60:100, 0.65:100, 0.70: 100).

In some embodiments, in the reaction system of the enzymatic hydrolysis, the ratio of the subtilisin to the crude silk protein is: 0.25-0.75:100 (e.g., 0.30:100, 0.35:100, 0.40:100, 0.45:100, 0.50:100, 0.55:100, 0.60:100, 0.65:100, 0.70: 100).

In some embodiments, the time for enzymatic hydrolysis is 2-5 hours.

In some embodiments, following the enzymatic hydrolysis, the papain and/or the subtilisin is subjected to an enzyme deactivation treatment.

In some embodiments, the enzyme inactivation treatment is performed in a water bath at 80-95 ℃ for 5-15 min.

In some embodiments, after the enzymatic hydrolysis, the reaction mixture is centrifuged to obtain a supernatant, and the crude silk peptide solution is obtained.

In some embodiments, the centrifugation conditions are: 3-7 ℃, 4000-8000 r-min ^-1 ，20-40min。

In some embodiments, the silkworm cocoons are subjected to degumming treatment to obtain the crude silk protein.

In some embodiments, the degumming step is: boiling the silkworm cocoon with water, and carrying out primary drying on the boiled silkworm cocoon to obtain the silkworm cocoon crude protein.

In some embodiments, the boiled silkworm cocoons are washed clean with distilled water and then subjected to primary drying to obtain the silkworm cocoon crude protein.

In some embodiments, the weight ratio of the silkworm cocoons to the water during the boiling is 1: 30-50.

In some embodiments, the boiling time is 1-3 h.

In some embodiments, the boiled cocoons are boiled again with water and then subjected to primary drying.

In some embodiments, the first drying is 100 ℃ oven drying.

In some embodiments, the silkworm cocoons are washed clean and subjected to secondary drying before degumming.

In some embodiments, the second drying is 100 ℃ oven drying.

In some embodiments, after said degumming, said silkworm cocoon crude protein is added to saturated CaCl ₂ Boiling the aqueous solution with ice, and filtering to obtain a first filtrate.

In some embodiments, the silkworm cocoon crude protein is the saturated CaCl at 20-30 ℃ ₂ Substances in aqueous solutionThe concentration of the amount percentage is 3-7%.

In some embodiments, the boiling time is 10-20 min.

In some embodiments, the first filtrate is subjected to membrane separation, and the filtrate is lyophilized to obtain lyophilized silkworm cocoon crude protein.

In some embodiments, the crude solution of silk peptide is decolorized to yield a decolorized fibroin solution.

In some embodiments, the decolorizing is performed using biochar.

In some embodiments, the biochar is gulfweed biochar.

In some embodiments, the volume to weight ratio of the crude solution of silk peptide to the biochar is 1 ml: 30-50 g.

In some embodiments, the gulfweed biochar is obtained by subjecting gulfweed to a high temperature treatment.

In some embodiments, the method for preparing the gulfweed biochar is as follows: uniformly mixing the dried sargassum with alkali, treating at the temperature of 150 ℃ and 250 ℃ for 20-40min, and treating at the temperature of 600 ℃ and 800 ℃ for 90-150min to obtain the sargassum biochar.

In some embodiments, the decolorized fibroin solution is lyophilized to obtain a fibroin lyophilized powder.

In a second aspect, the present invention provides a silk peptide prepared by the method of the first aspect of the present invention.

In a third aspect the present invention provides a composition comprising: silk peptide, arbutin and auxiliary materials in the second aspect of the invention.

In some embodiments, the composition comprises, in parts by weight: 15-35 parts (such as 16 parts, 18 parts, 20 parts, 22 parts, 24 parts, 26 parts, 28 parts, 30 parts, 32 parts and 34 parts) of the silk fibroin, 2-8 parts (such as 3 parts, 4 parts, 5 parts, 6 parts and 7 parts) of arbutin and 59-91 parts (such as 60 parts, 62 parts, 64 parts, 66 parts, 68 parts, 70 parts, 72 parts, 74 parts, 76 parts, 78 parts, 80 parts, 82 parts, 84 parts, 86 parts, 88 parts and 90 parts) of auxiliary materials.

In some embodiments, the composition comprises, in parts by weight: 20-30 parts of silk peptide, 3-5 parts of arbutin and 66-80 parts of auxiliary material.

In some embodiments, the composition comprises, in parts by weight: 30 parts of silk peptide, 4 parts of arbutin and 73 parts of auxiliary material.

In some embodiments, the adjuvants are 4-16 parts humectant and 55-75 parts water, by weight in the composition.

In some embodiments, the adjuvants are 6-10 parts of humectant and 60-70 parts of water, by weight in the composition.

In some embodiments, the adjuvant is 8 parts humectant and 65 parts water by weight in the composition.

In some embodiments, the humectant comprises glycerin, isopropyl myristate, or a combination thereof.

In some embodiments, the humectant comprises 2-8 parts glycerin, 2-8 parts isopropyl myristate, by weight in the composition.

In some embodiments, the humectant comprises 3-5 parts glycerin, 3-5 parts isopropyl myristate, by weight in the composition.

In some embodiments, the humectant comprises 4 parts glycerin, 4 parts isopropyl myristate, by weight in the composition.

In a fourth aspect, the present invention provides a process for the preparation of a composition according to the third aspect of the invention, said process comprising the steps of: mixing the silk peptide, the arbutin and the auxiliary material to obtain the composition.

In some embodiments, the composition is sterilized to obtain a sterilized composition.

In some embodiments, the sterilization is filter sterilization.

In some embodiments, the filter sterilization is performed using a 0.22-0.45 μm pore size filter.

In a fifth aspect, the present invention provides a method of producing a silk peptide according to the first aspect of the present invention, a silk peptide according to the second aspect of the present invention, a composition according to the third aspect of the present invention, or a method of producing a composition according to the fourth aspect of the present invention, and the use thereof in the preparation of an antibacterial agent.

In some embodiments, the antimicrobial formulation is an anti-acne cosmetic.

The invention has the advantages that:

the arbutin is a natural active substance derived from green plants, integrates the green plants, the safe and reliable and the efficient decoloration into a whole, can quickly permeate into the skin, can effectively inhibit the activity of tyrosinase in the skin and block the formation of melanin while not influencing the cell proliferation concentration, accelerates the decomposition and excretion of the melanin by directly combining with the tyrosinase by self, thereby reducing the skin pigmentation, removing color spots and freckles, and does not generate toxic, irritant, sensitizing and other side effects on the melanocyte, and also has the functions of sterilization and inflammation diminishing.

Isopropyl myristate has effects of keeping moisture and penetrating, and skin has good absorption to the product, and can effectively contact hair follicle in cortex, penetrate into deep cortex, bring active components into cosmetics, and give full play to the effect of effective components.

As a natural animal protein, the silk has great similarity with human skin and fur, and compared with other vegetable proteins, the silk protein is easier to be absorbed by the human skin, thereby having the effects of moisturizing, whitening and protecting the skin. Fibroin is a complete protein, contains various amino acids required by human body, and has good repairing and nourishing effects on human skin and hair. The tyrosine, phenylalanine and the like with high content have good absorption effect on ultraviolet rays, and have the functions of sun protection and skin care; and tyrosine can inhibit the formation of tyrosinase, prevent melanin precipitation, and has whitening effect. In addition, the treated fibroin contains residues of various polar amino acids, and the residues are Natural Moisturizing Factors (NMF), have good moisture absorption and retention, can prevent excessive evaporation of skin moisture, and can moderately moisturize the skin, so that the silk fibroin has good anti-wrinkle and anti-aging effects.

The abuse of antibiotics causes various problems of antibiotic residues in food, the appearance of super bacteria, the improvement of drug resistance of human bodies and the like, the development of safe and efficient antibiotic substitutes is very important, and the acquisition of natural antibacterial substances from organisms is an important research direction. The antibiotic substitutes which are difficult to generate drug resistance and safe are mainly found to be antibacterial peptide, oligosaccharide, probiotics and the like, and can be used in various products such as food, feed, medicines and the like.

The antibacterial Peptide is also called Antimicrobial Peptide (Antimicrobial peptides) or Peptide antibiotics (Peptide antibiotics) and is a small molecular Peptide (the molecular mass is less than 10ku) of the natural immune system of organisms, and the generation of the small molecule is originated from the defense of the organisms to pathogens, so that the antibacterial Peptide has a wide antibacterial spectrum and has an inhibitory effect on fungi and most gram-negative and positive bacteria. In addition, the antibacterial peptide also has the characteristics of high sterilization speed, difficult hydrolysis by protease and the like. Compared with other proteins, the antibacterial peptide has environmental protection and wider antibacterial spectrum, can be used as a food preservative and feed for livestock breeding industry, and has a far-reaching scientific research prospect.

Therefore, various excellent functions of the silk antibacterial peptide are transferred to clothes, quilts and cosmetics in the morning of human beings, and the skin of the human beings is cared, so that the human beings have healthy skin and accord with the green consumption concept of the human beings in the new century. Therefore, effective extraction and use of the silk antibacterial peptide are always top core technologies in the field of high-grade cosmetics.

Detailed Description

Embodiments of the present invention will be described in further detail below.

Example 1: preparation of silk antibacterial peptide

The preparation method of the silk antibacterial peptide comprises the following steps:

(1) pretreatment of silkworms: collecting silkworm cocoon, selecting diseased silkworm and bad silkworm, and removing silkworm feces and impurities; cutting off cocoon shell, removing pupa body, repeatedly cleaning with distilled water for 3-5 times, and oven drying at 100 deg.C for 5 hr.

(2) Degumming process: weighing 75g of the sample dried in step (1), and shearing the cocoon into about 1-3cm ² And after the fragments are broken, placing the fragments in 3L of distilled water, heating and boiling for degumming in an electric heating constant temperature furnace for 2h, taking out the samples, changing water, repeatedly boiling for 2h, taking out the degummed silk, washing the degummed silk with 23 ℃ distilled water for multiple times, and then placing the degummed silk in a 100 ℃ drying oven for drying for 5 h.

(3) And (3) a salt dissolving process: saturated CaCl at 25 DEG C ₂ Heating the aqueous solution to boiling state, and adding the sample obtained in the step (2) into boiling saturated CaCl ₂ Aqueous solution (sample with CaCl) ₂ The weight-volume ratio of the solution is 1 g: 22ml), stirring, keeping the solution slightly boiling for 15min after the sample is completely dissolved, filtering with a terylene filter cloth while the solution is hot, and collecting the filtrate.

(4) And (3) membrane separation process: desalting the filtrate obtained in the step (3) for 30h by using an ultrafiltration membrane to obtain a sample without calcium and magnesium ions, and freeze-drying to obtain the crude fibroin.

(5) And (3) an enzymolysis process: preparing a silk crude protein aqueous solution with the mass percentage of 5.5%, reducing the pH value to 7.0 at 50 ℃, adding papain and subtilisin for enzymolysis for 3h, mixing the papain and the subtilisin and the like, wherein the total amount of the added protease is 1.0% of the weight of the freeze-dried silk crude protein sample. Heating the enzymolysis solution at 90 deg.C for 10min to inactivate enzyme, cooling to room temperature, centrifuging at 8000r/min for 30min, and collecting supernatant.

(6) And (3) decoloring: and (5) decoloring the supernatant sample obtained in the step (5) by using charcoal. Firstly, washing and soaking the gulfweed by deionized water to remove impurities and attachments on the surface of the gulfweed, drying the gulfweed in an oven at 105 ℃ to constant weight, and crushing the gulfweed by a crusher to pass through a 100-mesh sieve. Uniformly mixing the sieved undersize sample and KOH according to the mass ratio of 1:2, putting the mixture into a crucible, putting the crucible into a tubular furnace in a high-purity nitrogen (99.999%) atmosphere at the nitrogen flow rate of 100mL/min, continuously heating to 200 ℃ at the heating rate of 10 ℃/min, keeping the temperature for 30min, heating to 700 ℃ at the same heating rate, and pyrolyzing for 2 h; setting the cooling rate to 10 ℃/min after the pyrolysis is finished, naturally cooling the product to 500 ℃, taking out the product after cooling, washing the product to be neutral by using deionized water, and drying the product in an oven at 80 ℃ for 24 hours to obtain the modified gulfweed-based biochar. And (3) adding the sample obtained in the step (5) and gulfweed biochar (volume to weight ratio is 1ml:30g) into a decoloring stirring kettle, stirring at 25 ℃ and normal pressure for 30-60min, and filtering to remove gulfweed-based biochar to obtain colorless filtrate.

(7) And (3) freeze drying: and (3) mixing water, the sample obtained in the step (6) and a freeze-drying protective agent according to a mass ratio of 5: 1: 0.1 to obtain a dispersion. Taking 2ml of the prepared dispersion liquid, adding the dispersion liquid into a penicillin bottle of 10ml, moving the penicillin bottle, placing the penicillin bottle into a low temperature box at the temperature of-20 ℃, and freezing for 24h to completely freeze free water in the sample into ice. Subsequently, the pressure was reduced to 5Pa, the temperature was reduced to-40 ℃ and the freezing time was 24 h. And obtaining corresponding freeze-dried powder after freezing. Wherein the freeze-drying protective agent is 5 wt% of sucrose freeze-drying protective agent.

Example 2: preparation of composition containing silk antibacterial peptide

According to the parts by weight, 20 parts of silk antimicrobial peptide freeze-dried powder, 3 parts of arbutin, 3 parts of glycerol, 3 parts of isopropyl myristate and 60 parts of injection water in the step (7) in the example 1 are taken. The above formula is dissolved and mixed uniformly, and then filtered and sterilized by a 0.45 mu m microporous filter membrane, and filled and capped according to 15 ml/bottle.

Examples 3-10 compositions were prepared as in example 1, except that the amounts of materials were varied.

The weight parts of the raw materials of the compositions with silk antimicrobial peptide in examples 2-10 are shown in table 1.

TABLE 1 weight parts amounts of materials for different example compositions

15-35 parts of silk peptide, 2-8 parts of arbutin and 59-91 parts of auxiliary materials. The auxiliary materials comprise 4-16 parts of humectant and 55-75 parts of water.

20-30 parts of silk peptide, 3-5 parts of arbutin and 66-80 parts of auxiliary materials. The auxiliary materials comprise 6-10 parts of humectant and 60-70 parts of water.

30 parts of silk peptide, 4 parts of arbutin and 73 parts of auxiliary material. The auxiliary materials are that the humectant comprises 2-8 parts of glycerol and 2-8 parts of isopropyl myristate.

Test example 1: test of antibacterial property of silk antibacterial peptide

The antibacterial performance of the silk antibacterial peptide freeze-dried powder (five parts of A0, A1, A2, A3 and A4) prepared in the manner described in example 1 was tested, and different tests were respectively carried out.

Test samples: taking 4 parts of 1.0g silk antibacterial peptide freeze-dried powder, and diluting to 100ml with citric acid-sodium citrate buffer solution (0.1mol/L, pH6.0) to obtain test sample solutions with numbers of A1, A2, A3 and A4.

Test bacteria: respectively inoculating nutrient agar slant culture of Escherichia coli (A1), Staphylococcus aureus (A2), Malassezia (A3), and Propionibacterium acnes (A4) on nutrient agar slant, culturing at 35-37 deg.C for 24 hr, washing thallus Porphyrae with 0.9% sterilized NaCl aqueous solution, diluting with 0.9% sterilized NaCl aqueous solution, and making into 1 × 10 ⁷ -1×10 ⁸ cfu/ml of bacterial suspension ready for use.

4 bottles containing 200ml of sterilized solid nutrient agar culture medium are aseptically added with 4ml of the test sample solution in each bottle at 90-95 ℃, 1 bottle without the test sample solution (A0) is used as a blank control, then the plate is poured, the mixture is placed at room temperature and is placed at 35-37 ℃ for 24h, and the sterility is observed for later use.

Taking the prepared bacterial suspensions of escherichia coli, staphylococcus aureus, malassezia and propionibacterium acnes under aseptic operation, respectively coating 0.10ml of the bacterial suspensions on solid nutrient agar culture media added with different test sample solutions, culturing for 24 hours at 35-37 ℃, observing, counting and counting the bacteriostasis rate, wherein the results are shown in the following table 3:

the inhibition rate is blank control colony number-sample colony number/blank control colony number multiplied by 100%.

TABLE 2 sample compositions and processing scenarios for different numbers

The results are shown in table 3 below.

TABLE 3 antimicrobial Rate of the samples

The experiment shows that the silk antibacterial peptide has stronger antibacterial action on gram-positive bacteria or gram-negative bacteria, which provides a foundation for preparing acne-removing skin care products in the next step.

Test example 2: testing of the antimicrobial Properties of the compositions

The skin care products prepared in examples 2-10 were tested for their antibacterial properties:

test samples: the skin care products (compositions) prepared in examples 2 to 10 were prepared by taking 1ml of each test sample, and diluting to 100ml with a citric acid-sodium citrate buffer solution (0.1mol/L, pH6.0) to prepare test sample solutions.

Test bacteria: respectively inoculating nutrient agar slant culture of Escherichia coli, Staphylococcus aureus, and Propionibacterium acnes on the nutrient agar slant, culturing at 35-37 deg.C for 24 hr, washing thallus Porphyrae with 0.9% sterilized NaCl solution, diluting to 1 × 10 ⁷ -1×10 ⁸ cfu/ml of bacterial suspension for later use;

for each bacterium, 9 bottles containing 200ml of sterilized solid nutrient agar culture medium are aseptically added with 4ml of the test sample solution into each bottle at 70-80 ℃, 1 bottle without the test sample solution is used as a blank control, then the plate is poured, the mixture is placed at room temperature, incubated at 35-37 ℃ for 24h, and observed to be aseptic for later use.

Taking prepared bacterial suspensions of escherichia coli, staphylococcus aureus and propionibacterium acnes under aseptic operation, respectively coating 0.1ml of the bacterial suspensions on solid nutrient agar culture media added with different test sample solutions, culturing for 24 hours at 35-37 ℃, observing and counting the bacteriostasis rate, wherein the bacteriostasis rate is blank control colony number-sample colony number/blank control colony number multiplied by 100%. The results are shown in table 4 below.

TABLE 4 antimicrobial Effect of different example compositions on different strains of bacteria

	Escherichia coli	Staphylococcus aureus	Propionibacterium acnes
				Example 2	90％	91％	89％
Example 3	92％	88％	95％
				Example 4	93％	90％	91％
Example 5	89％	90％	92％
				Example 6	94％	92％	90％
Example 7	96％	95％	95％
				Example 8	93％	94％	91％
Example 9	90％	89％	93％
				Example 10	92％	94％	95％

As can be seen from the data shown in table 4, the skin care products prepared in examples 2 to 10 of the present invention have good antibacterial effect, and the skin care product prepared in example 7 has the best comprehensive antibacterial effect, has antibacterial and anti-inflammatory effects, and can exert the acne removing effect when used for skin care.

Test example 3: testing of the Combustion of the compositions

5g of the composition of example 2 was placed in a crucible, heated by an alcohol burner, and the combustion state of the composition was observed. The results show that the composition burns without significant splashing, has a strong smell without choking, and has no residue and oil after burning.

It will be appreciated by those skilled in the art that the invention may be embodied in other specific forms without departing from the spirit or essential characteristics thereof. The embodiments disclosed above are therefore to be considered in all respects as illustrative and not restrictive. All changes which come within the scope of or equivalence to the invention are intended to be embraced therein.

Claims

1. A method for preparing silk peptide, which is characterized by comprising the following steps:

degumming: degumming the silkworm cocoons to obtain the silk crude protein; the degumming step comprises the following steps: boiling the silkworm cocoons with water, changing the boiled silkworm cocoons with water and then boiling, washing the boiled silkworm cocoons with distilled water, and then carrying out primary drying to obtain the silkworm cocoon crude protein; in the boiling process, the weight ratio of the silkworm cocoons to the water is 1:30-50, and the boiling time is 1-3 h;

Before degumming, cleaning the silkworm cocoons, and drying for the second time;

salt dissolving: after degumming, adding the silkworm cocoon crude protein into saturated CaCl ₂ Boiling the aqueous solution, and then filtering to obtain a first filtrate; the silkworm cocoon crude protein is subjected to CaCl saturation at the temperature of 20-30 DEG C ₂ The mass percentage concentration of the water solution is 3-7%, and the boiling time is 10-20 min;

in the salt dissolving step, performing membrane separation on the first filtrate, and freeze-drying the filtrate to obtain freeze-dried silkworm cocoon crude protein;

enzymolysis: carrying out enzymolysis on the crude silk protein by using papain and subtilisin to obtain silk peptide; in the enzymolysis reaction system, the dosage and mass ratio of the papain to the silk crude protein are as follows: 0.25-0.75: 100; in the enzymolysis reaction system, the using amount and the mass ratio of the subtilisin to the silk crude protein are as follows: 0.25-0.75: 100; the enzymolysis time is 2-5 h;

the zymolytic substrate solution is a fibroin aqueous solution with the mass percentage concentration of 4-7%; the pH of the substrate solution for enzymolysis is = 4.0-8.0;

after the enzymolysis, carrying out enzyme deactivation treatment on the papain and the subtilisin, wherein the enzyme deactivation treatment condition is that the enzyme deactivation treatment is carried out in water bath at the temperature of 80-95 ℃ for 5-15 min; centrifuging the reaction system mixture, and taking supernatant to obtain a crude product solution of the silk peptide;

And (3) decoloring: decolorizing the crude silk peptide solution by using biochar to obtain a decolorized fibroin solution, wherein the biochar is gulfweed biochar; the volume weight ratio of the crude silk peptide solution to the biochar is 1 ml: 30-50 g; the gulfweed biochar is obtained by carrying out high-temperature treatment on gulfweed; the preparation method of the gulfweed biochar comprises the following steps: uniformly mixing the dried sargassum with alkali, treating at the temperature of 150-;

and (3) freeze-drying the decolorized fibroin solution to obtain the fibroin freeze-dried powder.

2. The method for producing a silk peptide according to claim 1, wherein the centrifugation conditions are: 3-7 ℃, 4000- ^-1 ，20-40min。

3. The method for producing silk peptide according to claim 1, wherein in the degumming step, the first drying is oven drying at 100 ℃.

4. The method for producing silk peptide according to claim 1, wherein the secondary drying is oven drying at 100 ℃ before the degumming.

5. A silk peptide produced by the method for producing a silk peptide according to any one of claims 1 to 4.

6. A composition, characterized in that the composition comprises: the silk peptide, arbutin and auxiliary materials as claimed in claim 5, wherein the composition comprises the following components in parts by weight: 15-35 parts of silk peptide, 2-8 parts of arbutin and 59-91 parts of auxiliary materials; the auxiliary materials comprise 4-16 parts of humectant and 55-75 parts of water; the humectant comprises 2-8 parts of glycerol and 2-8 parts of isopropyl myristate in parts by weight of the composition.

7. The composition of claim 6, wherein the composition comprises, in parts by weight: 20-30 parts of silk peptide, 3-5 parts of arbutin and 66-80 parts of auxiliary materials.

8. The composition of claim 7, wherein the composition comprises, in parts by weight: 30 parts of silk peptide, 4 parts of arbutin and 73 parts of auxiliary material.

9. The composition of claim 6, wherein the excipients comprise 6 to 10 parts by weight of humectant and 60 to 70 parts by weight of water in the composition.

10. The composition of claim 9, wherein the adjuvant is 8 parts by weight humectant and 65 parts by weight water in the composition.

11. The composition of claim 6, wherein the humectant comprises 3 to 5 parts by weight of glycerin and 3 to 5 parts by weight of isopropyl myristate, based on the parts by weight in the composition.

12. The composition of claim 11, wherein the humectant comprises 4 parts glycerol, 4 parts isopropyl myristate, by weight in the composition.

13. A method of preparing a composition as claimed in any one of claims 6 to 12, said composition comprising the steps of: mixing the silk peptide of claim 5, the arbutin and the auxiliary material to obtain the composition.

14. A process for preparing a composition according to claim 13, wherein said composition is sterilized to obtain a sterilized composition.

15. A process for the preparation of a composition according to claim 14, wherein the sterilization is filter sterilization; filtering with 0.22-0.45 μm filter membrane for sterilization.

16. Use of the silk peptide of claim 5, the composition of any one of claims 6 to 12 for the preparation of an antibacterial formulation.

17. The use of claim 16, wherein the antibacterial formulation is an anti-acne cosmetic.