CN105648008A - Feeding silk antibacterial peptide preparation and preparation method thereof - Google Patents
Feeding silk antibacterial peptide preparation and preparation method thereof Download PDFInfo
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- CN105648008A CN105648008A CN201610000658.1A CN201610000658A CN105648008A CN 105648008 A CN105648008 A CN 105648008A CN 201610000658 A CN201610000658 A CN 201610000658A CN 105648008 A CN105648008 A CN 105648008A
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- C07K14/43504—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates
- C07K14/43563—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates from insects
- C07K14/43586—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates from insects from silkworms
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Abstract
The invention discloses a feeding silk antibacterial peptide preparation and a preparation method thereof. The preparation method comprises the following steps of (1) degumming waste silk and dissolving calcium chloride of the degummed silk; (2) mixing the degummed silk solution prepared by the step (1) with the adopted Alcalase 2.4L and Flavorzyme according to the proportion of 3 to 2, controlling the reaction conditions that the pH is 8.5, the temperature is 55 DEG C and the reaction timeis 5h, and preparing silk polypeptide enzymatic hydrolysate; (3) dialyzing, desalting and concentrating the silk polypeptide enzymatic hydrolysate prepared by the step (2); (4) separating and purifying silk antibacterial peptide through DEAE-52, and Sephadex G-25 sephadex; (5) performing vacuum freeze drying on the silk antibacterial peptide solution prepared by the step (4), and obtaining a finished product. The invention aims to provide a green silk polypeptide preparation process which is used for replacing a feed supplement antibiotics and recovering the waste silk, and is simple in preparation process and easy to clean.
Description
Technical field
The invention belongs to animal feed additive technical field, be specifically related to a kind of feeding silkworm silk antimicrobial peptide preparation and preparation method thereof.
Background technology
The www.xinhuanet.com reported on November 23rd, 2015, was found that a kind of " superbacteria " gene that can resist potent antibiotics recently, it means that " last line of defense " in antibiotic used by the mankind has the risk being broken on domestic animal and the person. Research worker is analyzed the bacteria sample gathered with Carnis Sus domestica, Carnis Gallus domesticus and human patients and is found, there is a kind of special gene MCR-1, carry the antibacterial of this gene and polymyxin shown strong drug resistance, and this drug resistance can also fast transfer to other bacterial strains.
China feeds poultry owing to abusing various antibiotic, hormone and illegal drug, and cause that food safety affair takes place frequently, animal meat products export abroad declines year after year, China's Carnis Sus domestica, Fowl meat, fowl egg, first place, the meat total output Jun Zhan world, and the total volume of exports Jin Zhan world meat total volume of exports 3.6%, owing to animal feeding aspect yet suffers from safe and sanitary problem, the developed country such as European Union, the U.S., Japan does not release so far to China's animal sources type food import restriction. The biological feedstuff products substitution antibiotic of visible exploitation high-efficiency high-quality, hormone, production green non-pollution, organic meat, egg, milk are country and the livelihood issues of whole people's food safety needs.
In recent years, people have researched and developed the Substitutes For Antibiotics such as fodder enzyme preparation, Application of Direct-fed Microbials, acidulant, antibacterial peptide, oligosaccharide, garlicin, Chinese herbal medicine and organic metal trace element, these green feed additives obtain the favor of people, it is all based on suppressing harmful bacteria propagation, promotes probiotics propagation, improves and the basis of intestinal microenvironment carries out study, but on the whole, these antibiotic substitute products are for antibiotic, and its action effect still has a certain distance.
Various animals and poultry from immature ontogeny, the internal microorganism species just setting up balance, and have it to maintain the mechanism of self flora stable state, for instance Antioxidative Factors, hormone, albumen, little peptide etc. are to maintain the stable important factor of body microenvironment.
Antibacterial peptide is within 1980, to be separated, by Sweden scientist G.Boman et al., the class obtained in silkworm silk to have the basic polypeptide material of antibacterial activity, and molecular weight, about 2000 ~ 7000, is made up of 20 ~ 60 amino acid residues.This kind of active polypeptide majority has the features such as strong basicity, heat stability and broad-spectrum antiseptic. The present invention is with natural silk for raw material, the Activity and stabill of antibacterial peptide is ensure that by advanced technological design, project finally obtains to be had antibacterium, antifungal, the green of antioxidation isoreactivity, health, has no drug resistance, environmental friendliness, can replacing the antibiotic product of feedstuff, thus reaching better prevention and the purpose for the treatment of livestock and poultry.
Summary of the invention
It is an object of the invention to provide the preparation technology of the silkworm silk antibacterial peptide of a kind of simplicity, the customer service existing mold toxin sorbent unilateral absorbing mycotoxin of effect and the antibiotic pollution problem because using antibiotic to bring.
For above-mentioned purpose, technical scheme of the invention process is as follows:
The described antibacterial peptide that can produce antibacterial effect, is the raw material prepared as antibacterial peptide by the useless silkworm silk purchased from Bombyxmori Linnaeus raiser.
Described antibacterial peptide process of preparing is as follows:
(1) pretreatment of useless silkworm silk, weighs a certain amount of useless silkworm silk, utilizes sodium carbonate liquor that silkworm silk is carried out degumming process, the influence factor using sodium carbonate liquor concentration, solid-to-liquid ratio and refining time as useless silkworm silk pretreatment, the inspection target using degumming rate as experiment.
Described step (1) sodium carbonate liquor concentration is 0.2%-0.6%, and solid-to-liquid ratio is 1:30-1:70(g/mL), usually time 30-60min.
Described step (1) degumming rate, computational methods are to adopt the quality of sample before and after the process of weighting method after dried mensuration, respectively the fibroin sample of non-degumming and degumming is put in baking oven, dried to constant weight with the temperature of (65 �� 1) DEG C, and put in exsiccator and cool down, claim quality after at room temperature stablizing 12h with electronic balance respectively, obtain boiled silk and the dry weight of non-boiled silk, respectively with M1And M2Represent. Degumming rate formula is as follows:
In formula: M1Represent boiled silk dry weight, M2Non-boiled silk dry weight
(2) by boiled silk obtained for step (1), adopting calcium chloride solution to dissolve, the silkworm silk after dissolving adopts ultrafilter membrane that it is carried out ultrafiltration desalting processing.
In described step (2), calcium chloride solution concentration is 20%, 30%, 40%, 50%, and the ultrafilter membrane used is MWCO1000 ultrafilter membrane.
(3) step (2) is separated the boiled silk of the desalting processing obtained, adopts alkaline protease (Alcalase2.4L) and flavor protease (Flavorzyme) to be hydrolyzed.
The compound protease proportioning used in described step (3) is 1:1-1:3, controls concentration of substrate 3%-5%, enzyme concentration 1%-2.5%, pH8-9, reaction temperature 45 DEG C-65 DEG C, enzymolysis time 2.5h-6.5h. The present invention adopts the yield of Micro-kjoldahl method and formaldehyde method titration amino nitrogen simultaneous determination silkworm silk antibacterial peptide.
(4) the silkworm silk antibacterial peptide solution that step (3) is obtained through enzyme hydrolysis is easily separated purification, pure water is dialysed by the bag filter that silkworm silk enzymolysis solution is placed in, mainly by bag filter, macro-molecular protein is separated with micromolecular compound, after dialysis terminates, the 1/5 of original volume it is concentrated into again with polyethylene glycol 6000, collect standby, be the peptide material in silkworm silk.
(5) the silkworm silk polypeptide material adopting saturated ammonium sulfate settling step (4) to prepare, obtains silkworm silk antibacterial peptide precipitation.
(6) adopt calcium chloride to dissolve silkworm silk antibacterial peptide precipitation, adopt ultrafiltration membrance filter desalination and concentration silkworm silk antibacterial peptide.
The ultrafilter membrane that described step (6) uses is MWCO1000 film.
(7) the silkworm silk antibacterial peptide ion-exchange chromatography of concentrated mistake and sephadex chromatography are easily separated purification, adopt Coomassie brilliant blue colorimetry to be tracked detection, collect eluent.
Described step (7) adopts DEAE-52 cellulose that silkworm silk antibacterial peptide carries out preliminary separation purification, adopts Sephadex-G25 gel filtration chromatography to carry out further purification process,
(8) the eluting peak eluent that combining step (7) is collected, and according to the order number consecutively of eluting peak, after pre-freeze processes, adopt vacuum freeze drier to dry, obtain silkworm silk antibacterial peptide powder.
Described step (8) eluent thickness liquid level controls at 0.5-1cm, and eluent pre-freezing temperature is (-15 DEG C)-(-18 DEG C), and vacuum drier vacuum is 0.05MPa-0.08MPa, according to 0 DEG C of-50 DEG C of programmed cooling, drying time 12-24h.
(9) the invention provides the method for separating and preparing of a kind of silkworm silk antimicrobial peptide preparation, technique adopts enzymatic hydrolysis boiled silk, preparation process environment is gentle, separation process environmental protection, decrease because being the environmental pollution brought by organic reagent and energy source and power consumption, the silkworm silk antibacterial peptide that this technique separates preparation is adopted to may apply to feedstuff industry, because silkworm silk antibacterial peptide has anti-microbial property, so antibiotic composition in feed additive can substituted as antimicrobial component, so the preparation technology of silkworm silk antibacterial peptide product provided by the invention will have good development prospect.
Accompanying drawing explanation
Fig. 1 is silkworm silk antibacterial peptide preparation technology flow chart of the present invention.
Fig. 2 is DEAE-52 ion exchange column chromatographic grade elution curve (pillar specification: 20mm*65cm) of the present invention
Fig. 3 is SAP-1 component SephadexG-25 sephadex column chromatography elution curves figure (pillar specification specification: 16mm*100cm) of the present invention
Fig. 4 is silkworm silk antibacterial peptide SephadexG-25 polydextran gel Purity collection of illustrative plates (pillar specification specification: 16mm*100cm) of the present invention
Detailed description of the invention
Below by way of form of implementation, described above content of the present invention is further detailed, a kind of feeding silkworm silk antimicrobial peptide preparation and preparation method thereof, comprises the steps:
(1) analytical balance weighs the useless silkworm silk of 1000g, adds the sodium carbonate liquor 300mL of concentration 0.5%, extracts 40min, obtains boiled silk, using non-boiled silk as controlled trial.
(2) non-degumming step (1) obtained and the fibroin sample of degumming are put in baking oven, dried to constant weight with the temperature of (65 �� 1) DEG C, and put in exsiccator and cool down, claim quality respectively with electronic balance after at room temperature stablizing 12h, obtain boiled silk and the dry weight of non-boiled silk, respectively with M1And M2Represent, calculate degumming rate.
(3) boiled silk that the calcium chloride solution dissolving step (2) utilizing 40% obtains, adopts MWCO1000 ultrafilter membrane that boiled silk is carried out concentrating and desalinating process.
(4) alkaline protease (Alcalase2.4L) and compound protease (Flavorzyme) are mixed according to 3:2 ratio, add boiled silk concentrated solution prepared by the composite protease hydrolysis step (3) of 2%, control reaction bar pH8.5, temperature 55 DEG C, response time 5h, adopts the yield of Micro-kjoldahl method and formaldehyde method titration amino nitrogen simultaneous determination silkworm silk antibacterial peptide.
Described step (4) boiled silk contracting liquid concentration is 4%.
Described step (4) silkworm silk antibacterial peptide yield computational methods are as follows:
Protein content in boiled silk:
In formula: protein quality (g) in T---sample
V2---sample consumes sulphuric acid standard solution volume (mL)
V1---blank consumption sulphuric acid standard solution volume (mL)
C---sulphuric acid concentration of standard solution (mol/L)
The quality (g) of the nitrogen that 0.014---and 1.0mL sulphuric acid standard solution is suitable
6.25---nitrogen is scaled the coefficient of protein
W---sample quality (g)
V---sample decomposed solution cumulative volume (mL)
V'---sample decomposed solution distillation volume (mL)
Amino-acid nitrogen content:
In formula: free amino acid quality (g) total in A---sample
V1---blank assay is titrated to the volume (mL) of the standard solution of sodium hydroxide that terminal consumes after adding formaldehyde
V2---diluted sample is titrated to the volume (mL) of the terminal pH9.2 standard solution of sodium hydroxide consumed after adding formaldehyde
C---Concentration of Sodium Hydroxide Solution Standard (mol/L)
MM quality (g/mmol) of 0.014---nitrogen
The volume (mL) of V---test sample solution
The yield of silkworm silk antibacterial peptide:
(5) learnt from else's experience degumming and composite protease hydrolysis processed sample, it is placed in the bag filter (G-RC-18-2K) of molecular cut off 2000Da to pure water dialysis 48h, water is changed once saturating every 8h, after dialysis terminates, the 1/5 of original volume it is concentrated into again with polyethylene glycol 6000, collect standby, be silkworm silk polypeptide material concentrated solution.
(6) DEAE-52 ion exchange column initial gross separation purification step (5) the silkworm silk polypeptide concentrated solution prepared is adopted.
The described DEAE-52 ion exchange column specification of step (6) is 20mm*65cm.
The described DEAE-52 ion exchange column balance of step (6) adopts distilled water to be washed till neutrality.
The described DEAE-52 ion exchange column loading of step (6), silkworm silk polypeptide concentrated solution 5mL prepared by aspiration step (5) is in the equilibrated pillar of prior distilled water, at this moment pillar liquid outlet water check valve is opened, sample is made to be seeped in chromatography media, distilled water, each 100mL of 0.1mol/LNaCl, 0.3mol/LNaCl, 0.5mol/LNaCl is adopted to carry out gradient elution, flow speed control is at 0.5mL/min, it is tracked detection by Coomassie brilliant blue colorimetry, collects finally according to gradient elution curve and merge single eluting peak.
(7) eluent that step (6) is collected is placed in the bag filter (G-RC-18-2K) of molecular cut off 2000Da to pure water dialysis 48h, changes water every 8h once saturating, after dialysis terminates, vacuum lyophilization, obtain silkworm silk antibacterial peptide just separated powder.
(8) utilizing silkworm silk antibacterial peptide just separated powder prepared by distilled water dissolving step (7), to obtain final product, silkworm silk antibacterial peptide is separation solution just.
Silkworm silk antibacterial peptide described in step (8) just separation solution concentration is 1g/mL.
(9) SephadexG-25 sephadex column is utilized to be further purified silkworm silk antibacterial peptide just separation solution prepared by step (8).
The described SephadexG-25 sephadex column specification of step (9) is 16mm*100cm.
The described SephadexG-25 sephadex column of step (9), using front pillar to need to be balanced processing, adopt monophosphate monophosphate salt buffer balance columns of pH7.8, flow speed control is at 0.5mL/min, when the pH of effluent is consistent with level pad pH, chromatographic column reaches balance.
Quadrat method on the described SephadexG-25 sephadex column of step (9), silkworm silk antibacterial peptide prepared by aspiration step (8) just separation solution 5mL is in the prior pillar equilibrated with monophosphate monophosphate salt buffer, at this moment open pillar liquid outlet water check valve, make sample be seeped in chromatography media.
The described SephadexG-25 sephadex column eluting of step (9) and collection method, it is slowly added dropwise the distilled water of certain altitude with dropper when sample concave meniscus is close to post bed surface, prevent directly dripping eluent when eluting and destroy post bed surface, eluting is carried out with the monophosphate monophosphate salt buffer of 0.2mol/L, flow velocity 0.5mL/min, often 5mL collected by pipe, is tracked detection by Coomassie brilliant blue colorimetry, collects finally according to gradient elution curve and merges single eluting peak.
(10) eluting peak that combining step (9) is collected, at-16 DEG C of pre-freeze eluents, under no-load condition, carries out programmed cooling, time 1h according to temperature 0 DEG C ,-10 DEG C ,-20 DEG C ,-30 DEG C, at the dry 24h of vacuum 0.06MPa, obtains silkworm silk polypeptide powder.
Claims (6)
1. feeding silkworm silk antimicrobial peptide preparation and preparation method thereof, comprises the steps:
(1) useless silkworm silk utilize the sodium carbonate liquor that concentration is 0.2%-0.6% carry out degumming process;
(2) glue boiled silk prepared by calcium chloride dissolving step (1), utilizes protease to carry out enzymolysis;
(3) silkworm silk polypeptide solution prepared by dialysis, concentration, settling step (2);
(4) silkworm silk polypeptide solution prepared by ion-exchange chromatography and sephadex chromatography purification procedures (3) is utilized;
(5) silkworm silk antibacterial peptide solution prepared by step (4) is carried out lyophilization, obtain silkworm silk antibacterial peptide product.
2. useless natural silk degumming technique according to claim 1, it is characterised in that sodium carbonate liquor concentration 0.2%-0.6%, solid-to-liquid ratio are 1:30-1:70(g/mL), usually time 30-60min.
3. protease hydrolyzed technique according to claim 1, it is characterised in that compound protease proportioning is 1:1-1:3, controls concentration of substrate 3%-5%, enzyme concentration 1%-2.5%, pH8-9, reaction temperature 45 DEG C-65 DEG C, enzymolysis time 2.5h-6.5h.
4. step according to claim 1 (3), it is characterised in that bag filter molecular cut off 2000Da, adopt saturated ammonium sulphate silkworm silk polypeptide.
5. silkworm silk polypeptide separation purifying technique according to claim 1, it is characterized in that adopting DEAE-52, SephadexG-25 polydextran gel to be easily separated purification, DEAE-52 ion-exchange chromatography mobile phase adopts distilled water, 0.1mol/LNaCl, 0.3mol/LNaCl, 0.5mol/LNaCl, and flow speed control is at 0.5mL/min; SephadexG-25 sephadex column chromatographic flow adopts the monophosphate monophosphate salt buffer of pH7.8 mutually, and flow speed control is at 0.5mL/min.
6. silkworm silk antibacterial peptide drying process according to claim 1, it is characterised in that silkworm silk-16 DEG C of pre-freezes of antibacterial peptide solution, vacuum 0.06MPa, dry 24h.
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107699603A (en) * | 2017-11-28 | 2018-02-16 | 张夏洋 | A kind of extracting method of fibroin |
| CN108251480A (en) * | 2018-02-10 | 2018-07-06 | 海盐县凌特生物科技有限公司 | Remove the preparation method of the Functional Polypeptides of melanin in skin |
| CN110937595A (en) * | 2020-02-10 | 2020-03-31 | 江苏科技大学 | Method for preparing polypeptide-graphene composite material by utilizing silk fibroin oligopeptide water-phase stripping graphite and application thereof |
| CN111214385A (en) * | 2020-03-13 | 2020-06-02 | 珀莱雅化妆品股份有限公司 | Nanoparticle emulsion loaded with skin nutrient and preparation method thereof |
| CN113265438A (en) * | 2021-06-01 | 2021-08-17 | 广州市尚梓化工科技有限公司 | Preparation method of silk antibacterial peptide and application of silk antibacterial peptide in acne-removing cosmetics |
| CN117064811A (en) * | 2023-09-18 | 2023-11-17 | 广东省华桑丽皙生物技术有限公司 | Composition containing silk antibacterial peptide and application of composition in cosmetics |
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Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107699603A (en) * | 2017-11-28 | 2018-02-16 | 张夏洋 | A kind of extracting method of fibroin |
| CN108251480A (en) * | 2018-02-10 | 2018-07-06 | 海盐县凌特生物科技有限公司 | Remove the preparation method of the Functional Polypeptides of melanin in skin |
| CN110937595A (en) * | 2020-02-10 | 2020-03-31 | 江苏科技大学 | Method for preparing polypeptide-graphene composite material by utilizing silk fibroin oligopeptide water-phase stripping graphite and application thereof |
| CN110937595B (en) * | 2020-02-10 | 2023-04-18 | 江苏科技大学 | Method for preparing polypeptide-graphene composite material by utilizing silk fibroin oligopeptide water phase to strip graphite and application of polypeptide-graphene composite material |
| CN111214385A (en) * | 2020-03-13 | 2020-06-02 | 珀莱雅化妆品股份有限公司 | Nanoparticle emulsion loaded with skin nutrient and preparation method thereof |
| CN111214385B (en) * | 2020-03-13 | 2022-05-17 | 珀莱雅化妆品股份有限公司 | Nanoparticle emulsion loaded with skin nutrient and preparation method thereof |
| CN113265438A (en) * | 2021-06-01 | 2021-08-17 | 广州市尚梓化工科技有限公司 | Preparation method of silk antibacterial peptide and application of silk antibacterial peptide in acne-removing cosmetics |
| CN113265438B (en) * | 2021-06-01 | 2022-07-29 | 广州市尚梓化工科技有限公司 | Preparation method of silk antibacterial peptide and application of silk antibacterial peptide in acne-removing cosmetics |
| CN117064811A (en) * | 2023-09-18 | 2023-11-17 | 广东省华桑丽皙生物技术有限公司 | Composition containing silk antibacterial peptide and application of composition in cosmetics |
| CN117064811B (en) * | 2023-09-18 | 2024-04-12 | 广东省华桑丽皙生物技术有限公司 | Composition containing silk antibacterial peptide and application of composition in cosmetics |
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