CN204455079U - A kind of Cordyceps militaris (L.) Link. separation of fermentative broth is purified and concentrating unit - Google Patents
A kind of Cordyceps militaris (L.) Link. separation of fermentative broth is purified and concentrating unit Download PDFInfo
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- CN204455079U CN204455079U CN201520092436.8U CN201520092436U CN204455079U CN 204455079 U CN204455079 U CN 204455079U CN 201520092436 U CN201520092436 U CN 201520092436U CN 204455079 U CN204455079 U CN 204455079U
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Abstract
The utility model discloses a kind of Cordyceps militaris (L.) Link. separation of fermentative broth to purify and concentrating unit, it is characterized in that: comprise fermenting raw materials tank, whizzer, one-level ultra-filtration equipment, two-stage ultrafiltering device, nanofiltration concentrating unit, freeze drying plant, wherein said whizzer connects fermenting raw materials tank, one-level ultra-filtration equipment connects the clear liquid pipeline of whizzer, two-stage ultrafiltering device connects one-level ultra-filtration equipment dialyzate pipeline, nanofiltration concentrating unit connects the concentrated solution pipeline of two-stage ultrafiltering device, and freeze drying plant connects the concentrated solution pipeline of nanofiltration concentrating unit.Cordyceps militaris (L.) Link. separation of fermentative broth described in the utility model is purified and concentrating unit, + lyophilize combination process is concentrated by centrifugal+one-level ultrafiltration purification+two-stage ultrafiltering purifying and concentrated+nanofiltration, the impurity such as the macromolecular substance in Cordyceps militaris (L.) Link. fermented liquid, small molecular weight impurity, salt can well be removed, provide the concentration of effective constituent, have that separation and purification is effective, cycles of concentration advantages of higher.
Description
Technical field
The utility model relates to Cordyceps militaris (L.) Link. field, and particularly a kind of Cordyceps militaris (L.) Link. separation of fermentative broth is purified and concentrating unit.
Background technology
Cordyceps militaris (L.) Link., has another name called Cordyceps militaris (L.) Link., Cordyceps militaris etc., has some areas to be also called the Chinese caterpillar fungus that leaps up, and worldwide distribution natural resource quantity is little.By stroma (i.e. careless part) and sclerotium (i.e. the corpse part of worm) complex body dimerous.The traditional Chinese medical science is thought, Chinese caterpillar fungus enters lung kidney two warp, can tonifying lung cloudy, again can kidney-replenishing, cure mainly and suffer from a deficiency of the kidney, impotence and seminal emission, soreness of waist and knee joint, weak after being ill, chronic cough is weak, and phthisical cough phlegm blood, spontaneous sweating etc. are unique a kind of Chinese medicine that can balance simultaneously, regulate negative and positive.In Chinese caterpillar fungus, adenosine has antiviral, antibacterial, prophylactic treatment cerebral thrombosis, Intracerebral hemorrhage, suppresses Platelet congregation to prevent thrombosis, eliminates face spot, anti-ageing effect such as wrinkle resistant.
Cordyceps militaris (L.) Link. fermented liquid is through process, in solution, the solid content of effective constituent is about 1-2Brix, containing macromolecular albumen, colloid in solution, small molecules sugar and a small amount of salt, its effective constituent is Cordyceps polysaccharide and cordycepin, molecular weight is several thousand to less than 100,000, and the content of cordycepin is more much higher than Cordyceps sinensis, and Cordyceps militaris (L.) Link. is easy to artificial culture for Cordyceps sinensis simultaneously.At present, the extraction of Cordyceps polysaccharide, cordycepin, mainly adopt the technique such as ion exchange resin, macroporous resin adsorption, charcoal absorption, these technique ubiquity wasting of resources phenomenons are serious, energy consumption is high, with an organic solvent to shortcomings such as environments.
Thanking to red flag etc. at publication number is that it adopts microfiltration of ceramic membrane to remove suspended substance and macromole impurity for the Chinese invention patent application " a kind of adopt the method for membrane separation technique continuous production cordycepin and Cordyceps polysaccharide " of CN103214533A, and then obtain Cordyceps polysaccharide and cordycepin by Ultra filtration membrane, finally by alcohol crystal and recrystallization, obtain finished product.Above-mentioned technique can make technique in Cordyceps militaris (L.) Link. remove macromole impurity by ceramic membrane, but can not effectively remove small molecular weight impurity and salinity, make product moderate purity not high enough, and adopt organic solvent alcohol crystal and recrystallization, to environment, the shortcomings such as concentrated cost is high.
Summary of the invention
For the problems referred to above, it is high that the purpose of this utility model is to provide a kind of product purity, and concentrated effect is good, and running cost is low, and with an organic solvent etc. the Cordyceps militaris (L.) Link. separation of fermentative broth of advantage is not purified and concentrating unit.
For achieving the above object, the technical scheme that the utility model proposes is: a kind of Cordyceps militaris (L.) Link. separation of fermentative broth is purified and concentrating unit, it is characterized in that: comprise fermenting raw materials tank, whizzer, one-level ultra-filtration equipment, two-stage ultrafiltering device, nanofiltration concentrating unit, freeze drying plant, wherein said whizzer connects fermenting raw materials tank, one-level ultra-filtration equipment connects the clear liquid pipeline of whizzer, two-stage ultrafiltering device connects one-level ultra-filtration equipment dialyzate pipeline, nanofiltration concentrating unit connects the concentrated solution pipeline of two-stage ultrafiltering device, freeze drying plant connects the concentrated solution pipeline of nanofiltration concentrating unit, the ultra-filtration membrane molecular weight cut-off adopted in described one-level ultra-filtration equipment is 150K-200K dalton, and in two-stage ultrafiltering device, ultra-filtration membrane molecular weight cut-off is 500-1000 dalton, and in nanofiltration concentrating unit, the molecular weight cut-off of nanofiltration membrane is 150-300 dalton.
Further, the dialyzate outlet of described nanofiltration concentrating unit connects one-level ultra-filtration equipment by reflux line.
Adopt technique scheme, Cordyceps militaris (L.) Link. separation of fermentative broth described in the utility model is purified and concentrating unit, + lyophilize combination process is concentrated by centrifugal+one-level ultrafiltration purification+two-stage ultrafiltering purifying and concentrated+nanofiltration, the impurity such as the macromolecular substance in Cordyceps militaris (L.) Link. fermented liquid, small molecular weight impurity, salt can well be removed, provide the concentration of effective constituent, have that separation and purification is effective, cycles of concentration advantages of higher.
Accompanying drawing explanation
Fig. 1 is that Cordyceps militaris (L.) Link. separation of fermentative broth described in the utility model is purified and concentrating unit schematic diagram.
Embodiment
Below in conjunction with the drawings and specific embodiments, the utility model is described further.
As shown in Figure 1, Cordyceps militaris (L.) Link. separation of fermentative broth described in the utility model is purified and concentrating unit, comprise fermenting raw materials tank 10, whizzer 20, one-level ultra-filtration equipment 30, two-stage ultrafiltering device 40, nanofiltration concentrating unit 50, freeze drying plant 60, wherein whizzer 20 connects fermenting raw materials tank 10, one-level ultra-filtration equipment 30 connects the clear liquid pipeline of whizzer 20, two-stage ultrafiltering device 40 connects one-level ultra-filtration equipment 30 dialyzate pipeline, nanofiltration concentrating unit 50 connects the concentrated solution pipeline of two-stage ultrafiltering device 40, freeze drying plant 60 connects the concentrated solution pipeline of nanofiltration concentrating unit 50, the dialyzate outlet of nanofiltration concentrating unit 50 connects one-level ultra-filtration equipment 30 by reflux line 70, and the ultra-filtration membrane molecular weight cut-off adopted in one-level ultra-filtration equipment 30 is 150K-200K dalton, in two-stage ultrafiltering device 40, ultra-filtration membrane molecular weight cut-off is 500-1000 dalton, and in nanofiltration concentrating unit 50, the molecular weight cut-off of nanofiltration membrane is 150-300 dalton.
During concrete use, Cordyceps militaris (L.) Link. fermented liquid enters in whizzer after fully fermenting in fermentor tank and carries out centrifugal treating, obtain centrifuged supernatant, centrifuged supernatant enters in one-level ultra-filtration equipment, in one-level ultra-filtration equipment, ultra-filtration membrane molecular weight cut-off is 150K-200K dalton, macromole impurity is trapped, effective constituent and small molecular weight impurity, the compositions such as salt are through ultra-filtration membrane, enter dialyzate, dialyzate enters in two-stage ultrafiltering device and processes further, ultra-filtration membrane molecular weight cut-off in two-stage ultrafiltering device is 500-1000 dalton, effectively can remove small molecular weight impurity and part salt, then the concentrated solution of two-stage ultrafiltering device enters in nanofiltration concentrating unit and concentrates further, the molecular weight cut-off of nanofiltration membrane is 150-300 dalton, effective constituent is fully retained concentrated, the salt in solution can also be removed further, improve product purity, finally enter in freeze drying plant through the solution that nanofiltration is concentrated, obtain finished product.Cordyceps militaris (L.) Link. separation of fermentative broth described in the utility model is purified and concentrating unit, + lyophilize combination process is concentrated by centrifugal+one-level ultrafiltration purification+two-stage ultrafiltering purifying and concentrated+nanofiltration, the impurity such as the macromolecular substance in Cordyceps militaris (L.) Link. fermented liquid, small molecular weight impurity, salt can well be removed, provide the concentration of effective constituent, have that separation and purification is effective, cycles of concentration advantages of higher.
Although specifically show in conjunction with preferred embodiment and describe the utility model; but those skilled in the art should be understood that; not departing from the spirit and scope of the present utility model that appended claims limits; in the form and details the utility model is made a variety of changes, be protection domain of the present utility model.
Claims (2)
1. a Cordyceps militaris (L.) Link. separation of fermentative broth is purified and concentrating unit, it is characterized in that: comprise fermenting raw materials tank, whizzer, one-level ultra-filtration equipment, two-stage ultrafiltering device, nanofiltration concentrating unit, freeze drying plant, wherein said whizzer connects fermenting raw materials tank, one-level ultra-filtration equipment connects the clear liquid pipeline of whizzer, two-stage ultrafiltering device connects the dialyzate pipeline of one-level ultra-filtration equipment, nanofiltration concentrating unit connects the concentrated solution pipeline of two-stage ultrafiltering device, and freeze drying plant connects the concentrated solution pipeline of nanofiltration concentrating unit; The ultra-filtration membrane molecular weight cut-off adopted in described one-level ultra-filtration equipment is 150K-200K dalton, and in two-stage ultrafiltering device, ultra-filtration membrane molecular weight cut-off is 500-1000 dalton, and in nanofiltration concentrating unit, the molecular weight cut-off of nanofiltration membrane is 150-300 dalton.
2. Cordyceps militaris (L.) Link. separation of fermentative broth according to claim 1 is purified and concentrating unit, it is characterized in that: the dialyzate outlet of described nanofiltration concentrating unit connects one-level ultra-filtration equipment by reflux line.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201520092436.8U CN204455079U (en) | 2015-02-10 | 2015-02-10 | A kind of Cordyceps militaris (L.) Link. separation of fermentative broth is purified and concentrating unit |
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| CN201520092436.8U CN204455079U (en) | 2015-02-10 | 2015-02-10 | A kind of Cordyceps militaris (L.) Link. separation of fermentative broth is purified and concentrating unit |
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| CN204455079U true CN204455079U (en) | 2015-07-08 |
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106749140A (en) * | 2017-01-23 | 2017-05-31 | 同舟纵横（厦门）流体技术有限公司 | A kind of gibberellin high power concentration technology and concentration systems |
| CN106834086A (en) * | 2017-02-23 | 2017-06-13 | 浙江兰博生物科技股份有限公司 | A kind of separation of fermentative broth device |
| CN107287078A (en) * | 2017-08-24 | 2017-10-24 | 宜智萱（天津）生物科技有限公司 | A kind of yellow rice wine preparation method using Chinese caterpillar fungus fermentation supernatant as raw material |
| CN107467619A (en) * | 2017-08-24 | 2017-12-15 | 宜智萱（天津）生物科技有限公司 | A kind of preparation method of Chinese caterpillar fungus health soy sauce |
| CN109574395A (en) * | 2018-12-21 | 2019-04-05 | 江西国药有限责任公司 | A kind of purification of waste water deodorizing methods generated in fermentation cordyceps production process |
| CN109929008A (en) * | 2017-12-15 | 2019-06-25 | 江苏久吾高科技股份有限公司 | A kind of the UF membrane production method and device of pesticide |
| CN114891050A (en) * | 2022-04-25 | 2022-08-12 | 南京高新工大生物技术研究院有限公司 | Method for separating cordycepin from fermentation liquor or extracting solution |
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2015
- 2015-02-10 CN CN201520092436.8U patent/CN204455079U/en not_active Expired - Fee Related
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106749140A (en) * | 2017-01-23 | 2017-05-31 | 同舟纵横（厦门）流体技术有限公司 | A kind of gibberellin high power concentration technology and concentration systems |
| CN106834086A (en) * | 2017-02-23 | 2017-06-13 | 浙江兰博生物科技股份有限公司 | A kind of separation of fermentative broth device |
| CN107287078A (en) * | 2017-08-24 | 2017-10-24 | 宜智萱（天津）生物科技有限公司 | A kind of yellow rice wine preparation method using Chinese caterpillar fungus fermentation supernatant as raw material |
| CN107467619A (en) * | 2017-08-24 | 2017-12-15 | 宜智萱（天津）生物科技有限公司 | A kind of preparation method of Chinese caterpillar fungus health soy sauce |
| CN109929008A (en) * | 2017-12-15 | 2019-06-25 | 江苏久吾高科技股份有限公司 | A kind of the UF membrane production method and device of pesticide |
| CN109574395A (en) * | 2018-12-21 | 2019-04-05 | 江西国药有限责任公司 | A kind of purification of waste water deodorizing methods generated in fermentation cordyceps production process |
| CN109574395B (en) * | 2018-12-21 | 2021-12-10 | 江西国药有限责任公司 | Method for purifying and deodorizing wastewater generated in production process of fermented cordyceps sinensis powder |
| CN114891050A (en) * | 2022-04-25 | 2022-08-12 | 南京高新工大生物技术研究院有限公司 | Method for separating cordycepin from fermentation liquor or extracting solution |
| CN114891050B (en) * | 2022-04-25 | 2023-12-15 | 南京高新工大生物技术研究院有限公司 | Method for separating cordycepin from fermentation liquid or extracting liquid |
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