CN107236059A - Mucous membrane of small intestine accessory substance separated in synchronization extractive technique - Google Patents
Mucous membrane of small intestine accessory substance separated in synchronization extractive technique Download PDFInfo
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- CN107236059A CN107236059A CN201710680828.XA CN201710680828A CN107236059A CN 107236059 A CN107236059 A CN 107236059A CN 201710680828 A CN201710680828 A CN 201710680828A CN 107236059 A CN107236059 A CN 107236059A
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- protein
- salt solution
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- pig pancreas
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- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
- C08B37/006—Heteroglycans, i.e. polysaccharides having more than one sugar residue in the main chain in either alternating or less regular sequence; Gellans; Succinoglycans; Arabinogalactans; Tragacanth or gum tragacanth or traganth from Astragalus; Gum Karaya from Sterculia urens; Gum Ghatti from Anogeissus latifolia; Derivatives thereof
- C08B37/0063—Glycosaminoglycans or mucopolysaccharides, e.g. keratan sulfate; Derivatives thereof, e.g. fucoidan
- C08B37/0075—Heparin; Heparan sulfate; Derivatives thereof, e.g. heparosan; Purification or extraction methods thereof
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/06—Preparation of peptides or proteins produced by the hydrolysis of a peptide bond, e.g. hydrolysate products
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Abstract
Mucous membrane of small intestine accessory substance separated in synchronization extractive technique, is related to bio-pharmaceuticals and feed production technology field.The present invention extracts liquaemin using first salt solution, makes heparin and Separation of Proteins, extracts crude heparin sodium；Again by the protein produced in production, with being digested after fresh pancreas activation, protein peptides are produced.The product contains abundant small peptide and easily digested and assimilated by domestic animal, is a kind of animalsderived feedstuffs protein of new shape, the amino acid rich in short peptide chain and balance.Production performance and the general level of the health with enhancing animal particularly brood；With small peptide form exist amino acid and free amino acid have higher bioactivity, can as many animals protein feed source.
Description
Technical field
The present invention relates to bio-pharmaceuticals and feed production technology field.
Background technology
The most producers for extracting liquaemin from pig intestinal mucosa at present are all to produce liquaemin using following methods：
Intestinal mucosa adds the salt solution of 4 times of 3%~4% concentration of amount, adjusts PH7.5~8.5 to add 3g enzymes by every small intestine with sodium hydroxide solution
Ratio it is enzyme-added, and be warming up to 55 DEG C~58 DEG C, insulation enzymolysis is after 2 hours, the temperature for digesting mucous membrane risen to 80~85 DEG C, then
Insulation is filtered after 20 minutes, filtrate is cooled to 55 DEG C, adds resin in enzymolysis liquid in the ratio of every small intestine 15~20g resin
Adsorbing liquaemin, is adsorbed 8 hours.Filtering, filtrate discharge collects resin, by weight resin 1 to 1.2 with 20 Baume degrees salt solution
Elution is secondary.Collect and merge eluent addition qdx more than 80% alcohol precipitation, filtration twice, more than 90% dehydration of alcohol,
55~60 DEG C of drying liquaemins.
1kg goldbeater's skins are often produced using process above can produce sewage about 4kg, and sewage contains COD20000~25000, handled
Come cumbersome and costly, most producers can not all recycle, have to both have been wasted a large amount of as discharge of wastewater
Protein resource, and severe contamination environment constrains the development of the product.
The experiment done according to China Agricultural University is shown：Feed intestine membrane protein Gly-His-Lys, the daily gain of piglet and day feeding
Amount is similar to the piglet for feeding SDPP higher than the control group for feeding imported fish meal, and pig Scours index in 1~3 week is commented
Divide and significantly improve.It is introduced that dried porcine saluble replaces imported fish meal feeding broiler in the age in days stage of 22 ages in days~35, not only
Growth can be promoted, and efficiency of feed utilization can be improved.
The content of the invention
The present invention seeks to propose the method for production peptide albumen powder while liquaemin is extracted from pig intestinal mucosa, with
Intestinal mucosa resource is made full use of, blowdown is reduced.
The technical scheme is that：By pig intestinal mucosa with salt solution salt solution after, extract after crude heparin sodium, then by salt solution
The protein of generation is digested with the pig pancreas after activation, spray-dried acquirement polypeptide albumen powder.
The present invention extracts liquaemin using first salt solution, makes heparin and Separation of Proteins, extracts crude heparin sodium；Again will be raw
The protein produced in production, with being digested after fresh pancreas activation, produces protein peptides.The product contains abundant small peptide
Easily digested and assimilated by domestic animal, be a kind of animalsderived feedstuffs protein of new shape, the amino acid rich in short peptide chain and balance.Tool
There are the production performance and the general level of the health of enhancing animal particularly brood；The amino acid and free amino group existed in small peptide form
Acid have higher bioactivity, can as many animals protein feed source.Not only reduce environmental pollution but also improve production
And social benefit.
After new production technology, the problem of solving sewage discharge, while new product can be increased again.Using new
In sewage containing COD be only 7000~8000 after technique, compare post processing just save trouble, province's expense it is many.
100~110g Erepsin peptide can be obtained by raw material of 1200~1500g chitterlings, intestine membrane protein peptide price is
80000000 yuan~10000/kg, and using after the technique, the consumption of protease and alkali is also reduced, its cost is than original production
The technique of crude product heparin decreases.
The intestine membrane protein peptide produced using the technology of the present invention, the free amino acid containing abundant oligopeptides and balance, is one
High-quality protein raw material is planted, with stronger attractant, is easily digested and is absorbed by domestic animal, available for many animals and different hairs
The stage is educated, can be used widely in feedstuff industry, ad hoc proposal is used in piggy and sow feed or aquatic products material.
Pig pancreas after activation of the present invention is：Take fresh pig pancreas to wear into after pasty state with calcium ion activated dose to mix,
After stirring keep mixing temperature be 2~5 DEG C, soaking time be 6~8 hours after with protein mixed enzymolysis；The calcium
Ion-activated dose of addition quality accounts for the 0.1% of pasty state pig pancreas quality.
Calcium ion activated dose of preferably calcium chloride.
The temperature of spray drying of the present invention is 107 DEG C.There is no it in the product being made through 107 DEG C of high temperature spray-dryings
Its material, it is to avoid cross pollution.
In order to obtain protein after more salt solutions, to improve the yield of polypeptide albumen powder, during salt solution, in pig intestinal mucosa
The middle saline solution for adding 4 times of quality, the mass percent of saline solution is 5%~6%, and mixture is adjusted with sodium hydroxide
PH then heats to 55~58 DEG C to 8.0~8.5, salt solution 2 hours under heat-retaining condition.
The method that crude heparin sodium and protein after salt solution is efficiently separated is：Under normal pressure, salt solution product is warming up to
Boiling, makes protein coagulating, then is separated with centrifuge, and crude heparin sodium and protein are extracted respectively.
The protein that salt solution is produced is mixed with the pig pancreas after water, activation, then mixed system is warming up into 50~55 DEG C
Enzymolysis 1 hour.
It is preferred that salt solution protein and the water, the mixed material feeding mass ratio of pig pancreas after activation that produce be the ︰ 3 of 100 ︰ 20.
Embodiment
First, production technology：
1st, the good chitterlings of freshness are collected.
2nd, mucous membrane is extracted：By striking, mucous membrane of small intestine liquid is obtained.
3rd, salt solution：The common salt aqueous solution of 4 times of quality is added in mucous membrane of small intestine liquid, the concentration of saline solution is 5%~6%
(mass percent), the PH for adjusting mixture with sodium hydroxide then heats to 55~58 DEG C, under heat-retaining condition to 8.0~8.5
Salt solution 2 hours.
4th, separate：Under normal pressure, salt solution product is warming up to boiling, makes protein coagulating, then is separated with centrifuge, is obtained respectively
Filter liquor and filter residue.
5th, liquaemin is produced：The filter liquor that step 4 is obtained, which is cooled to after 55 DEG C, adds resin, enzymolysis liquid absorption heparin
Sodium, adsorbs 8 hours, then filters, filtrate discharge, collects resin.
By weight resin 1 to 1.2 with 20 Baume degrees salt waters twice.Collect and merge the qdx of eluent addition twice
More than 80% alcohol precipitation, filtration, more than 90% dehydration of alcohol, 55~60 DEG C of drying liquaemins.
6th, chitling protein peptide powder is produced：
1. pig pancreas is activated：Take fresh pig pancreas wear into after pasty state with calcium ion activated dose --- calcium chloride is mixed, chlorination
The addition quality of calcium accounts for the 0.1% of pasty state pig pancreas quality, and it is 2~5 DEG C, soaking time that mixing temperature is kept after stirring
To be used to digest after 6~8 hours.
2. the filter residue that obtains step 4 is digested to mix with the pig pancreas after water, activation, then mixed system is warming up to 50~
55 DEG C digest 1 hour.The mixed material feeding mass ratio of its filter residue and the pig pancreas after water, activation is the ︰ 3 of 100 ︰ 20.
3. dry：The enzymolysis liquid that step 8 is obtained is placed in 107 DEG C of spray dryer and handled, and obtains chitling protein peptides
Powder.
2nd, apply：
The dried porcine saluble for using above method to produce can replace the imported fish meal in laying ration with equivalent completely, and
Effect is better than using fish meal.
Claims (8)
1. mucous membrane of small intestine accessory substance separated in synchronization extractive technique, it is characterised in that by pig intestinal mucosa with salt solution salt solution after, extract
Go out after crude heparin sodium, then the protein that salt solution is produced is digested with the pig pancreas after activation, spray-dried acquirement is more
Peptide albumen powder.
2. method according to claim 1, it is characterised in that take fresh pig pancreas to wear into mixed with calcium ion activated dose after pasty state
Close, after stirring keep mixing temperature be 2~5 DEG C, soaking time be 6~8 hours after with protein mixed enzymolysis；It is described
Calcium ion activated dose of addition quality accounts for the 0.1% of pasty state pig pancreas quality.
3. method according to claim 2, it is characterised in that described calcium ion activated dose is calcium chloride.
4. method according to claim 1, it is characterised in that the temperature of the spray drying is 107 DEG C.
5. method according to claim 1, it is characterised in that during salt solution, adds the salt solution of 4 times of quality in pig intestinal mucosa
Solution, the mass percent of saline solution is 5%~6%, and the PH to 8.0~8.5, Ran Housheng of mixture is adjusted with sodium hydroxide
Temperature is to 55~58 DEG C, salt solution 2 hours under heat-retaining condition.
6. method according to claim 1, it is characterised in that under normal pressure, salt solution product is warming up to boiling, makes protein coagulating,
Separated again with centrifuge, crude heparin sodium and protein are extracted respectively.
7. method according to claim 1 or claim 2, it is characterised in that the protein for producing salt solution and the pig pancreas after water, activation
Dirty mixing, then by mixed system be warming up to 50~55 DEG C digest 1 hour.
8. method according to claim 7, it is characterised in that the protein that the salt solution is produced and the pig pancreas after water, activation
Mixed material feeding mass ratio be the ︰ 3 of 100 ︰ 20.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201710680828.XA CN107236059A (en) | 2017-08-10 | 2017-08-10 | Mucous membrane of small intestine accessory substance separated in synchronization extractive technique |
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| CN201710680828.XA CN107236059A (en) | 2017-08-10 | 2017-08-10 | Mucous membrane of small intestine accessory substance separated in synchronization extractive technique |
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| CN201710680828.XA Pending CN107236059A (en) | 2017-08-10 | 2017-08-10 | Mucous membrane of small intestine accessory substance separated in synchronization extractive technique |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108329405A (en) * | 2018-02-08 | 2018-07-27 | 黄石市典雅生物科技有限公司 | It is protected under a kind of resin adsorption state and the method for purified heparin sodium |
| WO2023170064A1 (en) | 2022-03-08 | 2023-09-14 | Horizon Ip, S.L | Heparin and mixtures of native proteins and peptides from waste tissue of slaughtered animals |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103349151A (en) * | 2013-07-19 | 2013-10-16 | 高邮市秦邮生物科技有限公司 | Method for extracting polypeptide protein power from mucous membrane of small intestine of pig |
-
2017
- 2017-08-10 CN CN201710680828.XA patent/CN107236059A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103349151A (en) * | 2013-07-19 | 2013-10-16 | 高邮市秦邮生物科技有限公司 | Method for extracting polypeptide protein power from mucous membrane of small intestine of pig |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108329405A (en) * | 2018-02-08 | 2018-07-27 | 黄石市典雅生物科技有限公司 | It is protected under a kind of resin adsorption state and the method for purified heparin sodium |
| WO2023170064A1 (en) | 2022-03-08 | 2023-09-14 | Horizon Ip, S.L | Heparin and mixtures of native proteins and peptides from waste tissue of slaughtered animals |
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