CN113264723B - High-performance green water permeable brick based on facultative aerobic microbe mineralization and preparation method thereof - Google Patents

High-performance green water permeable brick based on facultative aerobic microbe mineralization and preparation method thereof Download PDF

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CN113264723B
CN113264723B CN202110574784.9A CN202110574784A CN113264723B CN 113264723 B CN113264723 B CN 113264723B CN 202110574784 A CN202110574784 A CN 202110574784A CN 113264723 B CN113264723 B CN 113264723B
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CN113264723A (en
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张家广
韩瑞凯
周爱娟
刘元珍
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Taiyuan University of Technology
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    • C04—CEMENTS; CONCRETE; ARTIFICIAL STONE; CERAMICS; REFRACTORIES
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    • C04B28/00—Compositions of mortars, concrete or artificial stone, containing inorganic binders or the reaction product of an inorganic and an organic binder, e.g. polycarboxylate cements
    • C04B28/02—Compositions of mortars, concrete or artificial stone, containing inorganic binders or the reaction product of an inorganic and an organic binder, e.g. polycarboxylate cements containing hydraulic cements other than calcium sulfates
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    • C04—CEMENTS; CONCRETE; ARTIFICIAL STONE; CERAMICS; REFRACTORIES
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    • C04—CEMENTS; CONCRETE; ARTIFICIAL STONE; CERAMICS; REFRACTORIES
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    • C—CHEMISTRY; METALLURGY
    • C04—CEMENTS; CONCRETE; ARTIFICIAL STONE; CERAMICS; REFRACTORIES
    • C04B—LIME, MAGNESIA; SLAG; CEMENTS; COMPOSITIONS THEREOF, e.g. MORTARS, CONCRETE OR LIKE BUILDING MATERIALS; ARTIFICIAL STONE; CERAMICS; REFRACTORIES; TREATMENT OF NATURAL STONE
    • C04B2201/00—Mortars, concrete or artificial stone characterised by specific physical values
    • C04B2201/50—Mortars, concrete or artificial stone characterised by specific physical values for the mechanical strength

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Abstract

The invention discloses a high-performance green water permeable brick based on facultative aerobe mineralization and a preparation method thereof. In the preparation process, facultative aerobes are used as performance enhancers, and two doping modes of directly doping microorganisms and indirectly doping microorganisms by using recycled aggregates as carriers are adopted. In an aerobic environment, calcium carbonate is generated through aerobic metabolism of facultative aerobic bacteria to precipitate and repair cracks and holes of the recycled aggregate, and a bonding weak area between the recycled aggregate and a new cement matrix is enhanced; under the anoxic environment, calcium carbonate is generated through facultative aerobic bacteria in anaerobic metabolism to precipitate and repair closed holes in the water permeable bricks, so that the mechanical property of the water permeable bricks is enhanced, meanwhile, the water permeable performance is not greatly affected, and the high-performance green water permeable bricks with excellent mechanical property and water permeable performance are prepared by utilizing the building waste.

Description

High-performance green water permeable brick based on facultative aerobic microbe mineralization and preparation method thereof
Technical Field
The invention relates to the technical field of water permeable bricks, in particular to a high-performance green water permeable brick with good mechanical property and high water permeability, which is prepared based on a facultative aerobic microorganism mineralization method and construction waste.
Background
In recent years, china highly attaches importance to ecological civilization construction and proposes a sponge city development strategy for the first time in 2013. The water permeable brick is an important means for developing the sponge city strategy, and can effectively improve the urban ecological environment due to the unique pore structure, so that the city has good elasticity when adapting to environmental changes and facing natural disasters. Its advantages mainly include the following aspects: (1) Rainwater can permeate into the underground through the pores of the water permeable bricks, accumulated water and surface runoff formed on the surface of the ground can be effectively reduced when the rainfall is small, the peak flood time and the peak flood water quantity can be effectively shortened when the rainfall is large, and the problem of urban waterlogging is solved; (2) Urban underground water resources are supplemented, rainwater can permeate into the ground through the water permeable bricks and be stored, urban water circulation is promoted, and the problem of foundation settlement caused by excessive urban water consumption is effectively solved; (3) Can effectively alleviate the heat island effect in city because the porous nature of brick that permeates water, the moisture that the brick body was stored and the moisture of the soil body under the brick can evaporate ground through its hole when hot, reduces the earth's surface temperature in city to alleviate city heat island effect.
At present, the water permeable brick is not popularized on a large scale because the water permeable brick also has the following defects: (1) The water permeability coefficient and the mechanical property are a pair of contradictory indexes, and a water permeable brick with excellent mechanical property and water permeability is difficult to obtain; (2) A large number of semi-communicating holes and closed holes exist in the currently prepared water permeable brick, which has no obvious beneficial influence on water permeability, but can obviously reduce the mechanical property of the brick body; (3) The water permeable brick has certain water retention property, so that the frost resistance of the water permeable brick is low in northern cold regions; (4) Most of the existing methods for enhancing the performance of the water permeable bricks have high cost and are difficult to popularize and apply in projects.
The method for strengthening the performance of the water permeable brick adopted by researchers at home and abroad mainly comprises the following steps: and (1) doping PVA fibers into the water permeable bricks. The PVA fiber has excellent tensile strength, so that the development of cracks can be further limited after the brick body is cracked, and the mechanical property of the brick body is enhanced. Although the PVA fiber solves the contradiction between the mechanical property and the water permeability of the water permeable brick, the PVA fiber has higher cost and is difficult to be applied in large scale in engineering. (2) CaCO is added into water permeable bricks 3 And (4) nano slurry. CaCO 3 The nano slurry can fill micro cracks in the aggregate and form a polymer film on the surface to reduce the porosity of the recycled aggregate so as to reduce the water absorption, so that the overall fluidity of the slurry is improved and more slurry is distributed in the pores of the permeable brick, thereby improving the mechanical property of the permeable brick but greatly reducing the mechanical property of the permeable brickLow water permeability. And (3) using the waste rubber powder as a performance enhancer. In a certain waste rubber powder mixing amount range, the particle size of the waste rubber powder is smaller, and fine pores in the aggregate can be filled, so that the mechanical property of the water permeable brick is enhanced, and the number of communicating holes is obviously reduced, so that the water permeability of the water permeable brick is reduced. Most of the existing performance strengthening means are to adopt materials with smaller particle size to fill the pores in the water permeable bricks so as to strengthen the mechanical performance of the water permeable bricks, but the strengthening means often do not distinguish favorable pores and harmful pores in the water permeable bricks, the strengthening effect is too obvious, and the strengthening effect has obvious adverse effects on the water permeable performance while strengthening the mechanical performance.
Currently, with the modernization and the acceleration of the industrialization process and the renovation and transformation of old cities and long roads in China, 20 to 25 hundred million tons of construction waste accounts for 40 percent of urban solid waste in China every year, however, most of the construction waste in our country adopts an open-air stacking and landfill treatment mode, the reutilization rate is less than 10 percent, and the requirement of the national sustainable development strategy is not met.
Disclosure of Invention
The invention aims to solve the outstanding contradiction between the mechanical property and the water permeability of the current water permeable brick based on effectively utilizing the construction waste, and provides a high-performance water permeable brick which exerts the mineralization advantage of facultative aerobic microorganisms, has good mechanical property and water permeability, is green, environment-friendly and low in cost; the prepared water permeable brick belongs to a green environment-friendly novel building material, is composed of building garbage, cement, fly ash, mineralized microorganisms, nutrient substances and the like, and can realize good water permeability and sufficient mechanical property while fully realizing recycling of the building garbage.
The invention is realized by adopting the following technical scheme:
1. a high-performance green water permeable brick based on facultative aerobe mineralization is prepared from the following raw materials in parts by weight:
1080 to 1300 parts of recycled aggregate (serving as coarse aggregate) carrying the facultative aerobes and having the particle size of 4.75mm to 9.5mm, 720 to 870 parts of natural aggregate (serving as fine aggregate) having the particle size of 2.36mm to 4.75mm, 425 to 510 parts of cement, 75 to 90 parts of fly ash, 150 to 170 parts of water, 20 to 30 parts of facultative aerobes suspension and 7 to 10 parts of calcium lactate.
The recycled aggregate is I-type recycled coarse aggregate for concrete classified according to performance requirements in the specification 'recycled coarse aggregate for concrete'.
The performance enhancer is facultative aerobic microorganism and nutrient substance which have alkalophilicity, good mineralization and deposition capability under the condition of sufficient and insufficient oxygen and have the capability of generating calcium carbonate salt by taking an organic calcium source as a substrate through self metabolism, the facultative aerobic microorganism can mineralize and deposit a large amount of calcium carbonate sediment by utilizing self metabolism, and the sediment has good compatibility with a cement-based material.
2. Screening the facultative aerobic mixed bacteria:
a. preparing a sludge mixture: respectively sieving the concentrated tank sludge of the sewage treatment plant and the garden sludge, and uniformly mixing according to the weight ratio of 1:1.
b. Preparation of selection medium: aerobic microorganism selection culture solution: 1L of ultrapure water, 0.85g of sodium lactate (calculated by 60 percent), 0.02g of ammonium chloride and 10mL of trace element liquid; anaerobic microorganism selection culture solution: 1L of ultrapure water, 0.85g of sodium lactate (based on 60% content), 0.03g of sodium nitrate, and 10mL of a trace element liquid. Wherein, the culture medium C: N (carbon-nitrogen ratio) = 500.
c. Selecting and domesticating microorganisms: facultative aerobic characteristic microorganism: the culture mode is two stages, namely aerobic characteristic domestication and anaerobic characteristic domestication, aerobic culture is firstly carried out, then anaerobic culture is carried out, and the aerobic culture and the anaerobic culture are taken as a period, and 10 periods of continuous culture are carried out.
d. And centrifuging the obtained bacterial liquid for 20min by a centrifugal machine at the rotating speed of 4000r/min to obtain bacterial sludge.
e. Re-suspending the bacterial sludge in sterilized distilled water, and diluting until the concentration of the bacteria in the bacterial liquid is 3.0-5.0 × 10 7 one/mL.
3. The preparation method of the regenerated aggregate particles carrying the facultative aerobes comprises the following steps:
a. and adsorbing the bacterial suspension on the surface and the inner pores of the recycled aggregate by adopting a vacuum adsorption method.
b. And (3) placing the recycled aggregate in a baking oven at 45 ℃ for drying for 24 to 36h until the carrier reaches constant weight.
c. And (3) spraying a calcium lactate solution (with the concentration of 1.75-2.50g/mL) on the surfaces of the dried regenerated aggregate particles adsorbing the facultative aerobes, and then carrying out secondary drying.
4. The preparation method of the high-performance green water permeable brick based on the mineralization of facultative aerobes comprises the following steps:
a. diluting the bacterial sludge with water to make the concentration of the bacterial sludge be 3.0 to 5.0 multiplied by 10 7 And taking the calcium lactate in parts by weight per mL, adding the calcium lactate in parts by weight into the bacterial liquid, and stirring for 60s.
b. Pouring all the recycled aggregate, the natural aggregate, the fly ash and 70% of mixing water into a stirrer, stirring for 15s to wet the aggregate, pouring all the cement into the stirrer to form shells, stirring for about 30s, adding 30% of mixing water into the mixture, stirring the mixture into a paste for about 60s, and pouring the bacterial liquid added with calcium lactate into the stirrer to stir uniformly.
c. When the mixture is placed into a mould, a vibrating rod is used for vibrating the inner side of the mould, mechanical equipment is used for vibrating the inner side of the mould, so that bubbles in the slurry can be eliminated, finally a trowel is used for troweling the brick surface, the brick surface is placed on a pressure tester for static pressure forming, the forming pressure is 3.8MPa, the brick body is placed into a constant-temperature and constant-humidity curing chamber with standard curing temperature (20 +/-2 ℃) and humidity (relative humidity is more than 95%) for curing, and the mould is detached after 24 hours.
d. Putting the brick body into a curing pool with standard curing temperature (20 +/-2 ℃), injecting water until the brick body submerges the highest brick surface for 5cm, fixing a porous oxygen pipe at the bottom of the curing pool, introducing oxygen, and measuring the oxygen concentration in the water by using a dissolved oxygen tester to keep the oxygen concentration in the water at 40-60mg/L for performance enhancement. And 8-14d later, taking out the water permeable brick, placing the water permeable brick at the average temperature of 20 +/-2 ℃, covering the brick body with a water-retaining material or a straw curtain, watering the brick body properly, and curing for 14-20d to obtain the high-performance water permeable brick.
In the preparation process, facultative aerobes are used as performance enhancers of the water permeable bricks, and two doping modes of directly doping the microorganisms and indirectly doping the microorganisms by using recycled aggregates as carriers are adopted. In an aerobic environment, calcium carbonate is generated through aerobic metabolism of facultative aerobic bacteria to precipitate and repair cracks and holes of the recycled aggregate, and a bonding weak area between the recycled aggregate and a new cement matrix is enhanced; under an anoxic environment, facultative aerobes are metabolized in an anaerobic environment to generate calcium carbonate precipitates to repair closed holes in the water permeable bricks, so that the mechanical property of the water permeable bricks is enhanced, and meanwhile, the water permeable performance is not greatly influenced, and the high-performance green water permeable bricks with excellent mechanical property and water permeable performance are prepared by utilizing building wastes. The high-performance green water permeable brick prepared by the invention innovatively proposes that the mineralization reaction speed, the size and the type of crystals are controlled by adjusting parameters such as the microbial mixing amount, the nutrient substance mixing amount, the maintenance environment and the like, a micro-pore structure is filled, and a bonding weak area between the recycled aggregate and a new cement matrix is reinforced, so that the mechanical property and the durability of the water permeable brick are remarkably improved, and the water permeable performance of the water permeable brick is not obviously affected.
The high-performance green water permeable brick based on the facultative aerobe mineralization, which is prepared by the invention, has the following characteristics:
firstly, the high-performance water permeable bricks based on the mineralization and deposition of facultative aerobes realize the reutilization of construction wastes, accord with the green sustainable development strategy, and accord with the concepts of energy conservation, environmental protection, green and high efficiency of the society.
Secondly, the facultative aerobes used in the invention have strong alkali resistance (the facultative aerobes are subjected to alkali resistance domestication because the environment is strong alkali environment during aerobic and anaerobic reinforcement), can adapt to the high-alkali environment inside the permeable bricks, and can resist severe environment due to spore formation of the microorganisms in extreme environment.
Thirdly, the facultative aerobes used in the invention have two metabolic modes, namely aerobic respiration under aerobic condition and fermentation under anaerobic condition, and both metabolic modes can generate a large amount of CO 3 +2 ，Mineralized with calcium-containing matrix in the water permeable brick to deposit performance-enhancing medium calcium carbonate to fill harmful holes in the water permeable brickThereby enhancing the mechanical properties thereof.
Fourthly, the used cement mortar with porous surfaces provides protection for the metabolism and survival of facultative aerobes in the maintenance and service process of the water permeable bricks by using the recycled aggregate used by the invention.
Fifthly, in the preparation process of the high-performance water permeable brick, two bacterium doping modes are adopted, wherein one mode is that suspension of facultative aerobes is directly doped in the preparation process of the water permeable brick; the other mode is that the recycled aggregate is used as a carrier of the facultative aerobes, and the facultative aerobes are indirectly mixed into the water permeable bricks. The direct incorporation of facultative aerobes enables rapid filling and repair of closed and semi-interconnected pores in permeable bricks. The indirect doping of the facultative aerobes can repair the holes and cracks of the recycled aggregate. Both the two doping modes can enhance the bonding weak area between the recycled aggregate and the cement matrix.
Sixth, the coarse aggregate used in the water permeable brick prepared by the invention is a recycled aggregate formed by crushing building waste, although the apparent density of the recycled aggregate is reduced and the water absorption and crushing index are increased compared with natural aggregate, the porous characteristic of the surface of the recycled aggregate can be utilized to provide a suitable environment for metabolism and survival for facultative aerobes, and the surface defects of the aggregate can be repaired to improve the physical and mechanical properties and the binding power with cement-based materials.
The brick is reasonable in design, and the high-performance green water permeable brick based on the facultative aerobic microbial mineralization has excellent mechanical properties and water permeability; meanwhile, the water permeable brick fully realizes the reutilization of construction waste, meets the requirements of the current economic society on green and environment-friendly construction materials, and has good practical application value.
Detailed Description
The following is a detailed description of specific examples of the invention.
Example 1
A high-performance green water permeable brick based on facultative aerobe mineralization is prepared from the following raw materials in parts by weight:
1080 parts of recycled aggregate (I) carrying facultative aerobes and having the grain diameter of 4.75mm to 9.5mm, 720 parts of natural aggregate (sand) having the grain diameter of 2.36mm to 4.75mm, 425 parts of cement, 75 parts of fly ash, 150 parts of water, 20 parts of facultative aerobes suspension and 7 parts of calcium lactate.
The preparation process of the high-performance green water permeable brick based on the mineralization of facultative aerobes comprises the following steps:
i, screening facultative aerobic mixed bacteria:
a. preparation of sludge mixture
Respectively sieving the sludge in the concentration tank of the sewage treatment plant and the garden sludge, and uniformly mixing according to the weight ratio of 1:1.
b. Preparation of selection Medium
Aerobic microorganism selection culture solution: 1L of ultrapure water, 0.85g of sodium lactate (based on 60% content), 0.02g of ammonium chloride and 10mL of trace element liquid. Anaerobic microorganism selection culture solution: 1L of ultrapure water, 0.85g of sodium lactate (based on 60% content), 0.03g of sodium nitrate, and 10mL of a trace element liquid. Wherein, the culture medium C: N (carbon to nitrogen ratio) = 500.
Each liter of the microelement liquid comprises the following components: 0.2g of vitamin H, 0.2g of folic acid (vitamin B9), 6 g of vitamin B, 0.5g of nicotinic acid (vitamin B3), 0.5g of vitamin B, 0.01g of vitamin B, 0.5g of p-aminobenzoic acid and 0.5g of lipoic acid.
c. Selective acclimation of microorganisms
Facultative aerobic property microorganism: the culture mode is two stages, namely aerobic characteristic domestication and anaerobic characteristic domestication, aerobic culture is firstly carried out, then anaerobic culture is carried out, and the aerobic culture and the anaerobic culture are taken as a period, and 10 periods of continuous culture are carried out.
And (3) characteristic strengthening process: 10mL of the sludge mixture and 100mL of the aerobic microorganism selection culture medium were added to a 300mL conical flask in a ratio of 1 3 PO 4 Adjusting the pH value to 11, covering the bottle mouth with sterile cotton yarn, and culturing in a constant-temperature incubator at 33 ℃ for 1 to 3d; then taking the bottom bacterium liquid to perform passage enrichment under the same operation, transferring the bottom bacterium liquid to the same selective culture medium by 10 percent of inoculation amount, and repeating the operation; then the bacterial liquid is centrifuged and resuspended in a bottle, and 10 times of the resuspended bacterial liquid is added into the bottleSelecting a culture solution from anaerobic microorganisms with the volume of the bacteria liquid, adjusting the pH value to 11, blowing off for 10min by using nitrogen to remove oxygen, culturing for 2-4 d in a constant-temperature shaking incubator at 33 ℃ at a rotating speed of 120r/min, and then carrying out passage enrichment on the bacteria liquid at the bottom of a bottle under the same operation, wherein the period is one period.
d. And centrifuging the obtained bacterial liquid for 20 minutes by using a centrifugal machine at the rotating speed of 4000r/min to obtain bacterial sludge.
e. Re-suspending the bacterial sludge in sterilized distilled water, and diluting until the bacterial concentration in the bacterial liquid is 3.0 × 10 7 One per mL.
II, a preparation method of the regenerated aggregate particles loaded with the facultative aerobes comprises the following steps:
a. and adsorbing the bacterial suspension on the surface and the inner pores of the recycled aggregate by adopting a vacuum adsorption method, and keeping the vacuum pump for 30 minutes under the negative pressure of 0.6 MPa.
b. And (3) placing the recycled aggregate in a baking oven at 45 ℃ for drying for 24 to 36h until the carrier reaches constant weight.
c. The dried surface of the regenerated aggregate particles adsorbing the facultative aerobes was sprayed with a calcium lactate solution (1.75 g/mL), and then secondary drying was performed.
III, a preparation method of the high-performance green water permeable brick based on facultative aerobic microbe mineralization, which comprises the following steps:
a. diluting the bacterial sludge with water to a concentration of 3.0 × 10 7 Taking the calcium lactate per mL, adding the calcium lactate into the bacterial liquid according to the weight part, and stirring for 1 minute;
b. pouring all the recycled aggregate, the natural aggregate, the admixture (fly ash) and 70% of mixing water into a stirrer, stirring for 15s to wet the aggregate, pouring all the cement into the stirrer to form a shell, stirring for about 30s, adding 30% of mixing water into the shell, stirring the mixture into a paste for about 60s, and pouring the bacterial liquid added with calcium lactate into the stirrer to stir uniformly.
c. When the mixture is placed into a mold, a vibrating rod is used for vibrating the inner side of the mold, mechanical equipment is used for vibrating, so that bubbles in the slurry can be eliminated to the greatest extent, finally, a trowel is used for troweling the brick surface, then, the brick surface is placed on a pressure tester for static pressure forming, the forming pressure is 3.8MPa, the brick body is placed into a constant-temperature and constant-humidity curing chamber with standard curing temperature (20 +/-2 ℃) and humidity (relative humidity is more than 95%) for curing, and the mold is detached after 24 hours.
d. Putting the brick body into a curing pool with standard curing temperature (20 +/-2 ℃), injecting water until the brick body submerges the highest brick surface for 5cm, fixing a porous oxygen pipe at the bottom of the curing pool, introducing oxygen, and measuring the oxygen concentration in the water by using a dissolved oxygen tester to keep the oxygen concentration in the water at 40-60mg/L for performance enhancement. And 8d, taking out the water permeable brick, placing the water permeable brick at the average temperature of 20 +/-2 ℃, covering the brick body with a water retention material or a straw curtain, watering properly, and curing for 20d to obtain the high-performance water permeable brick.
The water permeability coefficient of the water permeable brick of the embodiment is more than or equal to 5 multiplied by 10 -2 cm/s, can meet the paving requirement that the average compression strength is not lower than 25 MPa.
Example 2
A high-performance green water permeable brick based on facultative aerobe mineralization is prepared from the following raw materials in parts by weight:
1120 parts of regenerated aggregate (I) which carries the facultative aerobe and has the grain diameter of 4.75mm to 9.5mm, 755 parts of natural aggregate (sand) with the grain diameter of 2.36mm to 4.75mm, 445 parts of cement, 78 parts of fly ash, 155 parts of water, 22 parts of facultative aerobe suspension and 7 parts of calcium lactate.
The preparation process of the high-performance green water permeable brick based on the mineralization of facultative aerobes comprises the following steps:
i, screening facultative aerobic mixed bacteria:
a. the test procedures of b, c and d are the same as in example 1.
e. Re-suspending the bacterial sludge in sterilized distilled water, and diluting until the bacterial concentration in the bacterial liquid is 5 × 10 7 one/mL.
II, a preparation method of the regenerated aggregate particles loaded with the facultative aerobes comprises the following steps:
a. the experimental procedure for b was the same as in example 1.
c. Spraying calcium lactate solution (2.50 g/mL) on the surface of the dried regenerated aggregate particles adsorbing the facultative aerobic mixed bacteria, and then carrying out secondary drying.
III, the preparation method of the high-performance green water permeable brick based on the facultative aerobe mineralization is the same as that in example 1.
The water permeability coefficient of the water permeable brick of the embodiment is more than or equal to 4.5 multiplied by 10 -2 cm/s, and can meet the paving requirement that the average compressive strength is not lower than 30 MPa.
Example 3
A high-performance green water permeable brick based on facultative aerobe mineralization is prepared from the following raw materials in parts by weight:
1180 parts of recycled aggregate (I) loaded with the facultative aerobe and having the grain diameter of 4.75mm to 9.5mm, 800 parts of natural aggregate (sand) having the grain diameter of 2.36mm to 4.75mm, 470 parts of cement, 82 parts of fly ash, 160 parts of water, 25 parts of facultative aerobe suspension and 8 parts of calcium lactate.
The preparation process of the high-performance green water permeable brick based on facultative aerobic microbe mineralization comprises the following steps:
the procedure of the screening test of facultative aerobic mixed bacteria is the same as that in example 1.
II, preparation method of the recycled aggregate granules carrying the facultative aerobes is the same as example 1.
III, a preparation method of the high-performance green water permeable brick based on facultative aerobic microbe mineralization:
a. diluting the bacterial sludge with water to a concentration of 5.0 × 10 7 Taking the calcium lactate per mL, adding the calcium lactate into the bacterial liquid according to the weight part, and stirring for 1 minute;
b. the test procedures of c and d are the same as those of example 1.
The water permeability coefficient of the water permeable brick of the embodiment is more than or equal to 4.8 multiplied by 10 -2 cm/s, and can meet the paving requirement that the average compressive strength is not lower than 30 MPa.
Example 4
A high-performance green water permeable brick based on facultative aerobe mineralization is prepared from the following raw materials in parts by weight:
1240 parts of recycled aggregate (I) which carries the facultative aerobes and has the grain diameter of 4.75mm to 9.5mm, 835 parts of natural aggregate (sand) with the grain diameter of 2.36mm to 4.75mm, 490 parts of cement, 86 parts of fly ash, 165 parts of water, 28 parts of facultative aerobes suspension and 9 parts of calcium lactate.
The preparation process of the high-performance green water permeable brick based on the mineralization of facultative aerobes comprises the following steps:
the procedure of the screening test of facultative aerobic mixed bacteria is the same as that in example 1.
II, preparation method of the recycled aggregate granules carrying the facultative aerobes is the same as example 1.
III, a preparation method of the high-performance green water permeable brick based on facultative aerobic microbe mineralization:
a. b the procedure was the same as in example 1.
c. The molding pressure was set to 4.2MPa, and the procedure was otherwise the same as in example 1.
d. The oxygen supply curing is set to 14d, the natural curing is set to 14d, and the rest of the flow is the same as that in example 1.
The water permeability coefficient of the water permeable brick of the embodiment is more than or equal to 4 multiplied by 10 -2 cm/s, can meet the paving requirement that the average compressive strength is not lower than 35 MPa.
Example 5
A high-performance green water permeable brick based on facultative aerobe mineralization is prepared from the following raw materials in parts by weight:
1300 parts of recycled aggregate (I) which carries the facultative aerobe and has the grain diameter of 4.75mm to 9.5mm, 870 parts of natural aggregate (sand) with the grain diameter of 2.36mm to 4.75mm, 510 parts of cement, 90 parts of fly ash, 170 parts of water, 30 parts of facultative aerobe suspension and 10 parts of calcium lactate.
I, screening facultative aerobic mixed bacteria:
a. the experimental procedures of b, c and d are the same as those of example 1.
e. Resuspending the bacterial sludge in sterilized distilled water, and diluting until the concentration of bacteria in bacterial liquid is 5 × 10 7 one/mL.
II, a preparation method of the regenerated aggregate particles loaded with the facultative aerobes comprises the following steps:
a. the experimental procedure for b was the same as in example 1.
c. Spraying calcium lactate solution (2.50 g/mL) on the surface of the dried regenerated aggregate particles adsorbing the facultative aerobic mixed bacteria, and then carrying out secondary drying.
III, a preparation method of the high-performance green water permeable brick based on facultative aerobic microbe mineralization:
a. diluting the bacterial sludge with water to a concentration of 5.0 × 10 7 Taking the calcium lactate per mL, adding the calcium lactate into the bacterial liquid according to the weight part, and stirring for 1 minute;
b the procedure was the same as in example 1.
c. The molding pressure was set to 4.2MPa, and the rest of the procedure was the same as in example 1.
d. The oxygen supply curing is set to 14d, the natural curing is set to 14d, and the rest of the flow is the same as that in example 1.
The water permeability coefficient of the water permeable brick of the embodiment is more than or equal to 3 multiplied by 10 -2 cm/s, can meet the paving requirement that the average compressive strength is not lower than 40 MPa.
TABLE 1 example parameters and Properties
Figure DEST_PATH_IMAGE001
It can be known that the water permeability coefficient and strength are influenced by the raw material component proportion of the water permeable brick, the number of facultative aerobic microorganisms, the oxygen introducing curing time, the amount of nutrient substances and the forming pressure. The water permeable bricks prepared in the embodiments 2, 3 and 4 can have both the water permeability coefficient and the mechanical property, and are preferred embodiments.
The high-performance green water permeable brick based on the facultative aerobe mineralization, which is prepared in the embodiment, has excellent mechanical properties and excellent water permeability. It uses facultative aerobes as the performance enhancer, and mineralizes and deposits the calcium carbonate precipitate by using organic calcium source as the matrix through the self metabolism, namely the fermentation action under the aerobic respiration and anaerobic conditions under the aerobic condition. Two bacterium doping modes are adopted in the preparation process, firstly, the bacterium is directly doped in the preparation process, namely, the facultative aerobe suspension is directly mixed into the water permeable brick; and the second is to use the recycled aggregate as a carrier for indirect doping, namely, a vacuum adsorption method is utilized to adsorb microorganisms into the pores of the old cement mortar of the recycled aggregate. Among the two bacteria doping methods, the former method utilizes the anaerobic property of facultative aerobes to accurately fill closed holes in the water permeable bricks; the latter utilizes the aerobics of the facultative aerobes to repair the pores and cracks of the recycled aggregate and controls the mineralized deposition amount of the calcium carbonate by controlling parameters such as oxygen flux, nutrient substance adding amount, facultative aerobes adding amount and the like to strengthen the bonding weak area between the recycled aggregate and the new cement matrix without generating negative influence on the water permeability of the water permeable brick.
The high-performance green water permeable brick based on the mineralization of the facultative aerobes is a water permeable brick material which fully utilizes the advantages of the recycled aggregate and the facultative aerobes, has excellent mechanical properties and water permeability, has engineering practical significance, and has great research value and application significance.
Finally, it should be noted that the above embodiments are only for illustrating the technical solutions of the present invention and not for limiting, and although the detailed description is made with reference to the embodiments of the present invention, it should be understood by those skilled in the art that modifications or equivalent substitutions may be made on the technical solutions of the present invention without departing from the spirit and scope of the technical solutions of the present invention, which shall be covered by the claims of the present invention.

Claims (2)

1. The utility model provides a high performance green brick that permeates water based on good oxygen microorganism of facultative mineralizes which characterized in that: the feed is prepared from the following raw materials in parts by weight:
1120 to 1240 parts of regenerated aggregate with the particle size of 4.75mm to 9.5mm, 755 to 835 parts of natural aggregate with the particle size of 2.36mm to 4.75mm, 445 to 490 parts of cement, 78 to 86 parts of fly ash, 155 to 165 parts of water, 22 to 28 parts of suspension of the facultative aerobe and 4736 parts of calcium lactate 7~9; wherein the concentration of the facultative aerobe suspension is 3.0-5.0 × 10 7 Per mL;
the facultative aerobes can mineralize and deposit calcium carbonate precipitates by taking an organic calcium source as a matrix through aerobic respiration under an aerobic condition and fermentation under an anaerobic condition;
the preparation method of the regenerated aggregate particles loaded with the facultative aerobes comprises the following steps:
(1) Screening facultative aerobic mixed bacteria:
1.1 preparation of sludge mixture
Respectively sieving the sludge in the concentration tank of the sewage treatment plant and the garden sludge, and then mixing the sieved sludge with the garden sludge according to the weight ratio of 1:1, uniformly mixing;
1.2 preparation of selection Medium
Aerobic microorganism selection culture solution: 1L of ultrapure water, 0.85g of sodium lactate, 0.02g of ammonium chloride and 10mL of trace element liquid; anaerobic microorganism selection culture solution: 1L of ultrapure water, 0.85g of sodium lactate, 0.03g of sodium nitrate and 10mL of trace elements; wherein, medium C: N = 500;
1.3 Selective acclimation of microorganisms
Facultative aerobic characteristic microorganism: the culture mode is two stages, namely aerobic characteristic strengthening and anaerobic characteristic strengthening; aerobic culture is carried out first, then anaerobic culture is carried out, and the aerobic culture is taken as a period, and the continuous culture lasts for 10 periods;
the process for enhancing the characteristics of facultative aerobes comprises the following steps: adding a sludge mixed solution and an aerobic microorganism selection culture solution according to the proportion of 1 3 PO 4 Adjusting the pH value to 11, covering the bottle mouth with sterile cotton yarn, and culturing in a constant-temperature incubator at 33 ℃ for 1 to 3d; then taking the bottom bacterium liquid to perform passage enrichment under the same operation, transferring the bottom bacterium liquid to the same selective culture medium by 10 percent of inoculum size, and repeating the operation; centrifuging the bacterial liquid, then resuspending the bacterial liquid in a bottle, adding 10 times of anaerobic microorganism selective culture liquid to the bottle, adjusting the pH to 11, blowing off the culture liquid for 10min by using nitrogen to remove oxygen, culturing the culture liquid for 2 to 4d at the rotating speed of 120r/min in a constant-temperature shaking incubator at 33 ℃, and then taking bottom bacterial liquid of the bottle to perform one-time passage enrichment under the same operation, wherein the period is one;
1.4, centrifuging the obtained bacterial liquid for 20 minutes by a centrifugal machine at the rotating speed of 4000r/min to obtain bacterial sludge;
1.5, resuspending the bacterial sludge in sterilized distilled water, and then diluting until the concentration of the bacteria in the bacterial liquid is 3.0-5.0 × 10 7 Per mL;
(2) Preparation of regenerated aggregate carrier carrying facultative aerobes
2.1, adsorbing the bacterial suspension on the surface and the inner pores of the recycled aggregate by adopting a vacuum adsorption method;
2.2, drying the protective carrier to a constant weight;
2.3, spraying a calcium lactate solution on the surfaces of the dried recycled aggregate particles adsorbing the facultative aerobic mixed bacteria, wherein the concentration of the calcium lactate solution is 1.75 to 2.50g/mL, and then carrying out secondary drying;
the forming process of the water permeable brick is as follows: firstly, the bacterial sludge is diluted by water to make the concentration of the bacterial sludge be 3.0 to 5.0 multiplied by 10 7 Taking per mL, adding the calcium lactate in parts by weight into the bacterial liquid, and stirring for 1min; secondly, pouring all the recycled aggregate, the natural aggregate, the admixture and 70% of mixing water into a stirrer, stirring for 15s to wet the aggregate, pouring all the cement into the stirrer to form a shell, stirring for 30s, adding 30% of mixing water into the shell, stirring the mixture into a pasty state for 60s, and pouring the bacterial liquid added with calcium lactate into the stirrer to stir uniformly; then, when the mixture is placed into a mold, vibrating the inner side of the mold by using a vibrating rod, vibrating by using mechanical equipment, troweling the brick surface, placing the brick surface on a pressure tester for static pressure forming at a forming pressure of 3.8MPa, placing the brick body into a constant-temperature and constant-humidity curing chamber with standard curing temperature and humidity for curing, and removing the mold after 24 hours; and finally, putting the brick body into a curing pool with standard curing temperature, injecting water until the brick body is 5cm below the highest brick surface, fixing a porous oxygen pipe at the bottom of the curing pool, introducing oxygen, measuring the oxygen concentration in the water by using a dissolved oxygen measuring instrument to keep the oxygen concentration in the water within 40-60mg/L for performance reinforcement, taking out the water permeable brick after 8-14d, placing the water permeable brick under the condition that the average temperature is 20 +/-2 ℃, covering the brick body by using a water retention material or a straw curtain, watering the brick body, and curing for 14-20d to obtain the high-performance water permeable brick.
2. The facultative aerobic microbe mineralization-based high-performance green water permeable brick according to claim 1, wherein: each liter of the microelement liquid comprises the following components: 0.2g of vitamin H, 0.2g of folic acid (vitamin B9), 6 g of vitamin B, 0.5g of nicotinic acid (vitamin B3), 0.5g of vitamin B, 0.01g of vitamin B, 0.5g of p-aminobenzoic acid and 0.5g of lipoic acid.
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