CN114873958B - Crack self-repairing concrete suitable for sewage treatment tank and preparation method thereof - Google Patents

Crack self-repairing concrete suitable for sewage treatment tank and preparation method thereof Download PDF

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CN114873958B
CN114873958B CN202210635392.3A CN202210635392A CN114873958B CN 114873958 B CN114873958 B CN 114873958B CN 202210635392 A CN202210635392 A CN 202210635392A CN 114873958 B CN114873958 B CN 114873958B
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CN114873958A (en
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张家广
高沛
孟庆玲
周爱娟
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Taiyuan University of Technology
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    • C—CHEMISTRY; METALLURGY
    • C04—CEMENTS; CONCRETE; ARTIFICIAL STONE; CERAMICS; REFRACTORIES
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    • C—CHEMISTRY; METALLURGY
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    • C04B20/00—Use of materials as fillers for mortars, concrete or artificial stone according to more than one of groups C04B14/00 - C04B18/00 and characterised by shape or grain distribution; Treatment of materials according to more than one of the groups C04B14/00 - C04B18/00 specially adapted to enhance their filling properties in mortars, concrete or artificial stone; Expanding or defibrillating materials
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    • C12N1/38—Chemical stimulation of growth or activity by addition of chemical compounds which are not essential growth factors; Stimulation of growth by removal of a chemical compound
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    • Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
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Abstract

The invention discloses a crack self-repairing concrete suitable for a sewage treatment tank and a preparation method thereof, and relates to the technical field of adsorption material preparation, wherein the crack self-repairing concrete comprises the following components in parts by mass: 1180-1320 parts of reinforced recycled coarse aggregate, 145-235 parts of recycled fine aggregate, 300-340 parts of cement, 520-610 parts of sand, 110-125 parts of fly ash and 170-200 parts of water, wherein 20-30% of recycled fine aggregate is loaded with nutrients. The invention comprises the following steps: preparing a sludge mixed solution; preparing a microelement solution; preparing a selection medium; domestication of aerobic characteristics; domestication of anaerobic characteristics; carrying out passage enrichment; preparing strains; reinforcing the recycled coarse aggregate; preparing a regenerated fine aggregate; self-repairing concrete is prepared. The self-repairing concrete used for the sewage treatment tank has reasonable design, can be self-repaired in the severe environment of the sewage tank, also meets certain mechanical property and impermeability, reduces economic cost, realizes sustainable development, and has great practical significance and application value.

Description

Crack self-repairing concrete suitable for sewage treatment tank and preparation method thereof
Technical Field
The invention relates to the technical field of concrete materials, in particular to the technical field of crack self-repairing concrete materials, and specifically relates to crack self-repairing concrete suitable for a sewage treatment tank and a preparation method thereof.
Background
With various factors such as rapid economic development and population growth, the amount of industrial wastewater and domestic sewage is increased year by year, so that the problem of urban environmental pollution is increasingly serious. To protect the environment and promote the sustainable development of urban economy, the construction of urban sewage treatment plants is gradually expanding.
Under urban sewage environment, the sewage treatment tank is generally in a buried type, semi-buried type, closed, semi-closed or open air form, is subjected to the effects of chemicals such as acid and alkali, industrial sewage, industrial atmosphere, ultraviolet rays, flow abrasion of solid particles, scouring and the like for a long time, has various complex corrosion forms such as acid and alkali corrosion, atmospheric corrosion, abrasion and physical corrosion of permeable spalling, microbial corrosion of bacteria and algae and the like, and can also generate physical damage of spalling and penetrating, thereby forming serious corrosion. The concrete in the sewage treatment pool is corroded by various acids, alkalis, organic matters, inorganic matters and microorganisms, and in addition, the mechanical impact, the abrasion and the climate factors function, so that the concrete corrosion mechanism is extremely complex.
From various investigation and research at home and abroad, various concrete structures of sewage treatment plants have corrosion phenomena with different degrees, so that the longest service time of the structures is not longer than 20 years, and even parts of the structures are seriously deteriorated after being used for less than ten years. Obviously, the existing concrete design method is not suitable for sewage treatment facilities.
The metabolic processes of microorganisms can be classified into aerobic and anaerobic, with aerobic metabolism occurring mainly at the surface layer of the water in the sewage treatment tank and anaerobic metabolism occurring mainly at the underwater site, so far it has been confirmed that the bacterial expression form of the genus thiobacillus is a major factor causing the aging of concrete under the sewage condition. Their metabolic products form sulfuric acid, attack the biological matrix of the concrete, causing loss of strength and binding force, whereas thiobacilli are only part of a broad and complex corrosion process. Sulfuric acid has been identified as a corrosive agent, not only in the corrosion of sewage, but also in the corrosion of sewage treatment plants. These threat factors, in addition to the low tensile strength of the concrete, increase the likelihood of cracking of the concrete, and the increased permeability of the concrete due to cracking exposes the steel bars to the sewage water, which exposure can lead to carbonization, a decrease in the acid-base value, and eventually prevent passivation of the steel bars.
The passive repairing method of the concrete crack mainly comprises the steps of epoxy resin reinforced grouting, gravity filling or epoxy injection by using cementing materials and the like. However, these methods have high maintenance cost and complex treatment procedures, are not suitable for large-area popularization and use, and cannot meet the requirements of actual work. At present, most scholars at home and abroad adopt pure bacteria as a microbial self-repairing agent, the preparation cost of the pure bacteria is high, and the cost of microbial repairing is intangibly increased.
Therefore, the problems that various concrete structures of the current sewage treatment plant are seriously corroded, the service life is low, the concrete is seriously aged under the water condition, the existing repairing method is high in maintenance cost, complex in treatment procedure, not suitable for popularization, and incapable of meeting actual work and the like are solved.
Disclosure of Invention
The invention provides a crack self-repairing concrete suitable for a sewage treatment tank and a preparation method thereof, which aim to solve a series of problems that the crack self-repairing of the existing sewage treatment tank protects microorganisms from high-alkali environment, the self-repairing is slow under severe and harsh conditions of the sewage tank, the repairing method for the sewage treatment tank is high in maintenance cost, complex in treatment procedure, not suitable for popularization, incapable of meeting actual work and the like.
The invention is realized by adopting the following technology:
the invention provides a crack self-repairing concrete suitable for a sewage treatment tank, which comprises the following components in parts by weight: 1180-1320 parts of recycled coarse aggregate with 5-7 d enhancement and 4.75-26.5 mm particle size, 145-235 parts of recycled fine aggregate with 2.36-4.75 mm particle size, 300-340 parts of cement, 520-610 parts of sand, 110-125 parts of fly ash and 170-200 parts of water, wherein the recycled coarse aggregate is adsorbed with a mixed bacteria suspension, lactic acid and calcium nitrate, 20-30% of the recycled fine aggregate carries nutrients, the nutrients are mixed solution of lactic acid and calcium nitrate, the concentration of the lactic acid is 55-65 g/L, and the concentration of the calcium nitrate is 55-65 g/L.
The concrete preparation process for the crack self-repairing concrete applicable to the sewage treatment tank comprises the following steps: the method comprises the following steps:
a, preparing a sludge mixed solution
Taking activated sludge and garden soil of a sewage treatment plant, sieving to remove impurities contained in the activated sludge and garden soil, wherein the mass ratio is 1:1, uniformly mixing to prepare a sludge mixed solution;
b, preparing microelement solution
Adding 0.2g of vitamin H, 0.2g of folic acid (vitamin B9), 1g of vitamin B6, 0.5g of vitamin B2, 0.5g of vitamin B1, 0.5g of nicotinic acid (vitamin B3), 0.5g of vitamin B5, 0.01g of vitamin B12, 0.5g of p-aminobenzoic acid and 0.5g of lipoic acid into each liter of distilled water, and diluting by 100 times when the distilled water is used after the preparation is finished;
c, preparing a selective medium
Adding 0.85g sodium lactate (60% content), 0.02g ammonium chloride and 10mL trace element solution prepared in the step b into each 1L sewage to prepare a liquid selection culture medium; wherein the sewage is selected from a sewage treatment plant;
d, domestication of aerobic characteristics
Taking a sludge mixed solution and a selective culture medium with the volume ratio of 1:10, and using Na 3 PO 4 Regulating pH to 11, covering with a membrane, sealing, culturing at 33 deg.C under shaking for 2d, collecting supernatant, and collecting the sludge mixed solution at the bottom to obtain sludge mixed solution subjected to primary aerobic characteristic domestication;
e, domestication of anaerobic characteristics
Taking the sludge mixed solution subjected to primary aerobic characteristic domestication prepared in the step d with the volume ratio of 1:10 and a selective culture medium, and using Na 3 PO 4 Regulating pH to 11, charging nitrogen, deoxidizing for 15min, sealing, culturing at constant temperature for 2d by shaking, collecting sludge mixed solution at the bottom of house supernatant, and preparing sludge mixed solution subjected to primary anaerobic characteristic domestication; in order to ensure that the screened mixed bacteria can resist the severe environment of a sewage treatment tank to form spores, sewage is adopted for culture and domestication;
f, passage enrichment
D, circulating the steps d and e for subculture enrichment, and taking bottle bottom bacterial liquid after ten periods of continuous culture; the damage of the sewage treatment pool structure occurs on the surface layer of the water surface and under the water, and the gas-liquid interface scoured by the polluted water is most serious according to different damage degrees of the corrosion of the sewage to the concrete in the prior study, so that facultative aerobic mixed bacteria are adopted;
g, preparing strain
Centrifuging the bottle bottom bacterial liquid obtained in the step f for 20min at 4000r/min, taking bacterial mud, re-suspending in sterile distilled water, and diluting until the bacterial concentration is 5.5-6.0X10 6 cfu/mL to obtain a suspension of the bacteria;
h, reinforcing the recycled coarse aggregate
Adsorbing the suspension of the mixed bacteria prepared in the step g into the surface and the internal pores of the recycled aggregate by using a vacuum adsorption method and utilizing the internal and external pressure difference, drying at 45+/-2 ℃ by adopting a drying box, and regularly spraying a mixed solution of lactic acid and calcium nitrate on the surface of the recycled aggregate particles for adsorbing facultative aerobiotic mixed bacteria, wherein the concentration of the lactic acid is 55-65 g/L, the concentration of the calcium nitrate is 55-65 g/L, continuously spraying and enhancing for 5-7 d, and secondarily drying at the same temperature to obtain the enhanced recycled coarse aggregate with the particle size of 4.75-26.5 mm; because the sewage treatment tank has certain water pressure and is easy to generate permeability burst, certain requirements are met on strength and impermeability, and the recycled coarse aggregate after 5-7 d enhancement is adopted as a carrier to carry microorganisms;
i, preparing the regenerated fine aggregate loaded with nutrient substances
Adopting a vacuum adsorption method, and utilizing the internal and external pressure difference to adsorb the mixed solution of lactic acid and calcium nitrate into the surface and internal pores of the recycled fine aggregate to prepare the recycled fine aggregate with the particle size of 2.36-4.75 mm; the concentration of the lactic acid is 55-65 g/L, and the concentration of the calcium nitrate is 55-65 g/L. Because a part of available nutrient substances exist in the sewage, 20-30% of the recycled fine aggregate is used for fixedly supporting the nutrient substances, the rest of the recycled fine aggregate is not used for fixedly supporting the nutrient substances, and a smaller water-cement ratio is adopted, so that the mechanical property of the concrete is ensured, and the cost for inputting the nutrient substances is saved;
j, preparing self-repairing concrete
Adding 1180-1320 parts of the reinforced recycled coarse aggregate, 145-235 parts of the recycled fine aggregate, 520-610 parts of sand and 40-220 parts of water obtained in the step h into a stirrer, and stirring for 30s; 300-340 parts of cement, 110-125 parts of fly ash and the rest of water are added into a stirrer to be stirred for 3-4 min; wherein, 20-30% of 145-235 parts of the recycled fine aggregate is the recycled fine aggregate carrying the nutrient substances prepared in the step i. After the fly ash is doped in the concrete, the stress corrosion, the strength loss, the expansion rate and the rust resistance of the steel bar are all obviously improved under the load action of artificial sewage, so that the fly ash is added in the concrete to improve the performance of the concrete.
Compared with the prior art, the invention has the following beneficial effects: the self-repairing concrete for the cracks of the sewage treatment tank and the preparation method thereof, which are designed by the invention, adopt sewage for culture and domestication, and the cultured microorganisms can survive under the severe condition of the sewage treatment tank and can self-repair by utilizing organic matters and nutrient substances in the sewage, and only replace 20% -30% of regenerated fine aggregate to fixedly load the nutrient substances, thereby not only ensuring the mechanical property of the concrete, but also saving the cost of inputting the nutrient substances, and not only being capable of carrying out self-repairing of the cracks under the severe and severe environment of the sewage tank, but also meeting the enough mechanical property and seepage prevention property. The carrier is the recycled aggregate after 5-7 d reinforcement, namely, the surface pores of the recycled aggregate are utilized to provide attachment points for mixed bacteria, better environmental protection is provided for microorganisms through mineralization treatment reinforcement, and the mechanical property and the working property of the recycled concrete are improved. Not only the durability of the concrete structure is prolonged and the maintenance cost of the sewage treatment tank is reduced, but also the construction of the sewage treatment tank can protect the environment and promote the sustainable development of economy. The self-repairing concrete prepared by using the recycled aggregate as the carrier not only can solve the problem of shortage of natural sand and stone resources, but also can effectively solve the problems of stacking and pollution of the construction waste, realizes the recycling of the construction waste, and has very practical significance. The adopted microorganism is facultative aerobiotic mixed bacteria, and when oxygen is sufficient, namely the surface layer of the water surface can be subjected to aerobic metabolism; under the anaerobic environment, namely, the underwater structure can perform anaerobic metabolism, the mixed bacteria have more excellent mineralization deposition performance than pure bacteria, compared with pure bacteria, the mixed bacteria have more excellent mineralization deposition performance, the preparation cost of the strain is obviously reduced, and the characteristics of the strain are more consistent with the requirements of the actual concrete structure on the microbial restoration agent. The sewage can be used for culturing mixed bacteria and can be self-healed by utilizing nutrient substances and matrixes in the sewage when the sewage contacts concrete. Experiments prove that the self-repairing width of the designed test method reaches 0.65mm, 0.74mm and 0.83mm respectively through a 28-day underwater repairing and maintaining crack test, and can keep a higher crack complete repairing rate. The self-repairing concrete used for the sewage treatment tank has reasonable design, can be self-repaired in the severe environment of the sewage tank, also meets certain mechanical property and impermeability, reduces economic cost, realizes sustainable development, and has great practical significance and application value.
Drawings
FIG. 1 is an apparent map of the repair crack in water of example 1, a being before curing, b being repair curing 14d, c being repair curing 28d.
FIG. 2 is an apparent map of the repair crack in water of example 2, a being before curing, b being repair curing 14d, c being repair curing 28d.
FIG. 3 is an apparent map of the repair crack in water of example 3, a being before curing, b being repair curing 14d, c being repair curing 28d.
Detailed Description
Specific embodiments of the present invention will be described in detail below with reference to the accompanying drawings.
Example 1
The crack self-repairing concrete suitable for the sewage treatment pool comprises the following components in parts by mass: comprises the following components in parts by weight: 1180 parts of recycled coarse aggregate with the particle size of 4.75-26.5 mm, 145 parts of recycled fine aggregate with the particle size of 2.36-4.75 mm, 300 parts of cement, 575 parts of sand, 110 parts of fly ash and 170 parts of water are reinforced, the recycled coarse aggregate is adsorbed with a mixed bacterial suspension, lactic acid and calcium nitrate, 20-30% of the recycled fine aggregate is loaded with nutrient substances, and the nutrient substances are mixed solution of lactic acid and calcium nitrate, wherein the concentration of both lactic acid and calcium nitrate is 55g/L.
The preparation method of the crack self-repairing concrete suitable for the sewage treatment tank comprises the following specific steps:
a, preparing a sludge mixed solution
Taking activated sludge and garden soil of a sewage treatment plant, sieving to remove impurities contained in the activated sludge and garden soil, wherein the mass ratio is 1:1, uniformly mixing to prepare a sludge mixed solution;
b, preparing microelement solution
Adding 0.2g of vitamin H, 0.2g of folic acid, 1g of vitamin B6, 0.5g of vitamin B2, 0.5g of vitamin B1, 0.5g of nicotinic acid, 0.5g of vitamin B5, 0.01g of vitamin B12, 0.5g of p-aminobenzoic acid and 0.5g of lipoic acid into each liter of distilled water, and diluting 100 times when in use;
c, preparing a selective medium
Adding 0.85g sodium lactate (60% content), 0.02g ammonium chloride and 10mL trace element solution prepared in the step b into each 1L sewage to prepare a liquid selection culture medium;
d, domestication of aerobic characteristics
10mL of the sludge mixture and 100mL of the selective medium were added to a 150mL Erlenmeyer flask with Na 3 PO 4 Regulating the pH value to 11, covering the bottle mouth with a high temperature resistant tissue culture sealing film, culturing in a constant temperature shaking incubator at 33 ℃ for 2d, and taking the sludge mixed solution at the bottom from the supernatant of the house to prepare a sludge mixed solution subjected to primary aerobic characteristic domestication;
e, domestication of anaerobic characteristics
Taking 10mL of the sludge mixed solution subjected to primary aerobic characteristic domestication prepared in the step d, adding 100mL of a selection medium, and using Na 3 PO 4 Regulating pH to 11, filling nitrogen, deoxidizing for 15min, covering and sealing by a sealing film, culturing in a constant-temperature shaking incubator for 2d, and taking the sludge mixed solution at the bottom of the house supernatant to prepare the sludge mixed solution subjected to primary anaerobic characteristic domestication;
f, passage enrichment
C, performing passage enrichment in the circulating steps d and e, performing aerobic-anaerobic characteristic domestication for ten times, and taking bottle bottom bacterial liquid after ten periods of continuous culture;
g, preparing strain
Centrifuging the bottle bottom bacterial liquid obtained in the step f for 20min at 4000r/min, taking bacterial mud, re-suspending in sterile distilled water, and diluting until the bacterial concentration is 5.5-6.0X10 6 cfu/mL to obtain a suspension of the bacteria;
h, reinforcing the recycled coarse aggregate
Absorbing the suspension of the mixed bacteria prepared in the step g into the surface and the internal pores of the recycled aggregate by using the internal and external pressure difference, drying at 45+/-2 ℃ by adopting a drying box, regularly spraying a mixed solution of lactic acid and calcium nitrate on the surface of the recycled aggregate particles for absorbing facultative aerobiotic mixed bacteria, continuously spraying and enhancing the concentration of the lactic acid and the calcium nitrate for 5-7 d, and secondarily drying at the same temperature to obtain the enhanced recycled coarse aggregate with the particle size of 4.75-26.5 mm;
i, preparing the regenerated fine aggregate loaded with nutrient substances
Adopting a vacuum adsorption method, and utilizing the internal and external pressure difference to adsorb the mixed solution of lactic acid and calcium nitrate into the surface and internal pores of the recycled fine aggregate to prepare the recycled fine aggregate with the particle size of 2.36-4.75 mm; wherein the concentration of the lactic acid and the calcium nitrate is 55g/L; because a part of available nutrient substances exist in the sewage, 20-30% of the recycled fine aggregate is used for fixedly supporting the nutrient substances, and the rest of the recycled fine aggregate is not used for fixedly supporting the nutrient substances;
j, preparing self-repairing concrete
Adding 1180 parts of the reinforced recycled coarse aggregate, 145 parts of the recycled fine aggregate, 575 parts of sand and 170 parts of 40% of water (namely 68 parts of water) prepared in the step h into a stirrer, and stirring for 30s; adding 300 parts of cement, 110 parts of fly ash and the rest 102 parts of water into a stirrer, and stirring for 3-4 min; wherein 20-30% of 145 parts of the recycled fine aggregate is the recycled fine aggregate carrying the nutrient substances prepared in the step i.
The self-repairing effect of the cracks is tested, as shown in fig. 1, the width of the cracks to be repaired in water before maintenance is 0.65mm, after the repair is implemented, the cracks to be repaired in water are basically repaired at 14d, and the repair of the cracks to be repaired in water is completed at 28d.
Example 2
The crack self-repairing concrete suitable for the sewage treatment pool comprises the following components in parts by mass: comprises the following components in parts by weight: the method comprises the steps of reinforcing 1230 parts of recycled coarse aggregate with the grain size of 4.75-26.5 mm, 220 parts of recycled fine aggregate with the grain size of 2.36-4.75 mm, 315 parts of cement, 520 parts of sand, 115 parts of fly ash and 180 parts of water, wherein the recycled coarse aggregate is adsorbed with a mixed bacterial suspension, lactic acid and calcium nitrate, 20-30% of the recycled fine aggregate carries nutrients, and the nutrients are mixed solution of lactic acid and calcium nitrate, wherein the concentration of both lactic acid and calcium nitrate is 55g/L.
The preparation was the same as in example 1, except that the additive component in step j was added.
The self-repairing effect of the cracks is tested, as shown in fig. 2, the width of the cracks to be repaired in the water before maintenance is 0.74mm, after the repair is implemented, the cracks to be repaired in the water are basically repaired at 14d, and the repair of the cracks to be repaired in the water is completed at 28d.
Example 3
The crack self-repairing concrete suitable for the sewage treatment pool comprises the following components in parts by mass: comprises the following components in parts by weight: 1280 parts of regenerated coarse aggregate with 7d enhancement and particle size of 4.75-26.5 mm, 150 parts of regenerated fine aggregate with particle size of 2.36-4.75 mm, 330 parts of cement, 610 parts of sand, 120 parts of fly ash and 190 parts of water, wherein the regenerated coarse aggregate is adsorbed with a mixed bacterial suspension, lactic acid and calcium nitrate, 20-30% of the regenerated fine aggregate is loaded with nutrient substances, and the concentration of the nutrient lactic acid and the concentration of the calcium nitrate are 65g/L.
The concentrations of lactic acid and calcium nitrate in the nutrient substances in the steps h and i are 65g/L, and the additive component in the step j is prepared according to the above components in the same way as in example 1.
The self-repairing effect of the cracks is tested, as shown in fig. 3, the width of the cracks to be repaired in the water before maintenance is 0.83mm, and after the repair is implemented, the cracks to be repaired in the water still have gaps at 14d, and the repair of the cracks to be repaired in the water is completed at 28d.
Example 4
The crack self-repairing concrete suitable for the sewage treatment pool comprises the following components in parts by mass: comprises the following components in parts by weight: 1320 parts of 7d reinforced recycled coarse aggregate with the particle size of 4.75-26.5 mm, 235 parts of recycled fine aggregate with the particle size of 2.36-4.75 mm, 340 parts of cement, 545 parts of sand, 125 parts of fly ash and 200 parts of water, wherein the recycled coarse aggregate is adsorbed with a mixed bacterial suspension, lactic acid and calcium nitrate, 20-30% of the recycled fine aggregate carries nutrients, and the nutrients are mixed solution of lactic acid and calcium nitrate, and the concentration of the lactic acid and the calcium nitrate is 65g/L.
The preparation was the same as in example 3, except that the additive component in step j was added.
The scope of the present invention is not limited to the above embodiments, and various modifications and alterations of the present invention will become apparent to those skilled in the art, and any modifications, improvements and equivalents within the spirit and principle of the present invention are intended to be included in the scope of the present invention.

Claims (3)

1. A preparation method of crack self-repairing concrete suitable for a sewage treatment tank is characterized by comprising the following steps: the method comprises the following steps:
a, preparing a sludge mixed solution
Taking activated sludge and garden soil of a sewage treatment plant, sieving, wherein the mass ratio is 1:1, uniformly mixing to prepare a sludge mixed solution;
b, preparing microelement solution
Adding 0.2g of vitamin H, 0.2g of folic acid, 1g of vitamin B6, 0.5g of vitamin B2, 0.5g of vitamin B1, 0.5g of nicotinic acid, 0.5g of vitamin B5, 0.01g of vitamin B12, 0.5g of p-aminobenzoic acid and 0.5g of lipoic acid into each liter of distilled water, and diluting 100 times when in use;
c, preparing a selective medium
Adding 0.85g of sodium lactate, 0.02g of ammonium chloride and 10mL of the trace element solution prepared in the step b into each 1L of sewage to prepare a selective culture medium;
d, domestication of aerobic characteristics
Taking a sludge mixed solution and a selective culture medium with the volume ratio of 1:10, and using Na 3 PO 4 Regulating pH to 11, covering with a membrane, sealing, culturing at 33 deg.C under shaking for 2d, collecting supernatant, and collecting the sludge mixed solution at the bottom to obtain sludge mixed solution subjected to primary aerobic characteristic domestication;
e, domestication of anaerobic characteristics
Taking the sludge mixed solution subjected to primary aerobic characteristic domestication prepared in the step d with the volume ratio of 1:10 and a selective culture medium, and using Na 3 PO 4 Regulating pH to 11, charging nitrogen, deoxidizing for 15min, sealing, culturing at constant temperature for 2d by shaking, collecting sludge mixed solution at the bottom of house supernatant, and preparing sludge mixed solution subjected to primary anaerobic characteristic domestication;
f, passage enrichment
D, circulating the steps d and e for subculture enrichment, and taking bottle bottom bacterial liquid after ten periods of continuous culture;
g, preparing strain
Centrifuging the bottle bottom bacterial liquid obtained in the step f, taking bacterial mud, re-suspending in sterile distilled water, and diluting until the bacterial concentration is 5.5-6.0X10 6 cfu/mL to obtain a suspension of the bacteria;
h, reinforcing the recycled coarse aggregate
Absorbing the suspension of the mixed bacteria prepared in the step g to the surface and the internal pores of the recycled coarse aggregate by using an internal and external pressure difference by adopting a vacuum adsorption method, drying, regularly spraying nutrient substances on the surface of the recycled coarse aggregate particles for absorbing facultative aerobiotic mixed bacteria, continuously spraying and enhancing for 5-7 d, and secondarily drying to obtain the enhanced recycled coarse aggregate with the particle size of 4.75-26.5 mm;
i, preparing the regenerated fine aggregate loaded with nutrient substances
Adopting a vacuum adsorption method, and utilizing the internal and external pressure difference to adsorb nutrient substances into the surface and internal pores of the recycled fine aggregate to prepare the recycled fine aggregate with the particle size of 2.36-4.75 mm;
j, preparing self-repairing concrete
Adding 1180-1320 parts of the reinforced recycled coarse aggregate, 145-235 parts of the recycled fine aggregate, 520-610 parts of sand and 40-220 parts of water obtained in the step h into a stirrer, and stirring for 30s; 300-340 parts of cement, 110-125 parts of fly ash and the rest of water are added into a stirrer to be stirred for 3-4 min; wherein, 20-30% of 145-235 parts of the recycled fine aggregate is the recycled fine aggregate carrying the nutrient substances prepared in the step i.
2. The method for preparing the self-repairing concrete for the cracks of the sewage treatment pool, which is disclosed in claim 1, is characterized in that: in the step d, the constant-temperature shaking culture temperature is 33 ℃; in the step g, centrifuging for 20min at 4000 r/min; in the step h, drying is carried out at 45+/-2 ℃ by adopting a drying oven for two times, wherein the nutrient substances in the step h and the step i are mixed solutions of lactic acid and calcium nitrate, the concentration of the lactic acid is 55-65 g/L, and the concentration of the calcium nitrate is 55-65 g/L.
3. The utility model provides a crack self-repairing concrete suitable for sewage treatment pond which characterized in that: a preparation method according to any one of claims 1 or 2.
CN202210635392.3A 2022-06-07 2022-06-07 Crack self-repairing concrete suitable for sewage treatment tank and preparation method thereof Active CN114873958B (en)

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