CN113261499A - Chili pollen preservation and rapid cross breeding method - Google Patents
Chili pollen preservation and rapid cross breeding method Download PDFInfo
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- CN113261499A CN113261499A CN202110459474.2A CN202110459474A CN113261499A CN 113261499 A CN113261499 A CN 113261499A CN 202110459474 A CN202110459474 A CN 202110459474A CN 113261499 A CN113261499 A CN 113261499A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/02—Methods or apparatus for hybridisation; Artificial pollination ; Fertility
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N3/00—Preservation of plants or parts thereof, e.g. inhibiting evaporation, improvement of the appearance of leaves or protection against physical influences such as UV radiation using chemical compositions; Grafting wax
Abstract
The invention discloses a method for preserving pepper pollen and quickly hybridizing and breeding pepper pollen, which comprises the following steps: s1, pollen collection, namely collecting pollen with good development of the pepper in the flowering phase, and putting the collected pollen into a culture dish; s2, drying the pollen, namely putting the culture dish containing the pollen into a drying box, and drying the pollen to ensure that the water content of the pollen is 8-10%; s3, screening pollen, taking out the pollen, screening in a sterile room, and screening the agglomerated pollen again after extrusion; s4, pollen packaging, preparing a transparent centrifugal tube, placing the sieved pollen in the transparent centrifugal tube, placing moisture detection test paper in the transparent centrifugal tube, and sealing the transparent centrifugal tube; s5, pollen refrigeration, S6, pollen transportation, S7, pollen recovery, S8 and pollination. The invention can preserve the activity of pollen for a long time.
Description
Technical Field
The invention relates to the technical field of agriculture, in particular to a method for preserving pepper pollen and quickly hybridizing and breeding the pepper pollen.
Background
The problems of flowering asynchronism of parents and parents, excessive and less pollen due to weather, insufficient pollen quantity or low quality and the like are frequently encountered in the pepper hybrid seed production process, and the problems are solved, so that enough pollen can be prepared for high-quality pepper hybrid seed production.
The temperature is higher in the pollination period of pepper hybrid seed production generally in the daytime, if pollen is collected firstly and then hybrid pollination is carried out, the working efficiency is reduced, the pollination success rate is reduced due to the reduction of the pollen activity caused by high temperature, and meanwhile, the risk of heatstroke is increased for pollination workers.
The existing pepper pollen preservation method is suitable for short-distance transportation and short-term preservation, and the pollen activity is reduced along with the prolonging of time. The increase of water content also can't observe with the naked eye when pollen is preserved, need with the help of instrument and equipment, and troublesome poeration increases manual work and time cost.
Disclosure of Invention
The invention aims to provide a pepper pollen preservation and rapid cross breeding method, which collects pollen for pepper cross breeding in advance to enable the pollen to be reserved at any time, effectively solves the problems of flowering asynchronism of pepper parents and substandard pollen quantity and quality, provides enough pollen for pepper high-quality cross breeding, ensures the breeding foundation, can observe whether the water content of the preserved pollen is increased by naked eyes according to the change of a water detection test paper, reduces the time for measuring the water content by instruments and equipment and labor cost, and solves the problems in the background technology.
In order to achieve the purpose, the invention provides the following technical scheme:
a method for preserving pepper pollen and quickly crossbreeding pepper pollen comprises the following steps:
s1, pollen collection, namely collecting pollen with good development of the pepper in the flowering phase, and putting the collected pollen into a culture dish;
s2, drying the pollen, namely putting the culture dish containing the pollen into a drying box, and drying the pollen to ensure that the water content of the pollen is 8-10%;
s3, screening pollen, taking out the pollen, screening in a sterile room, and screening the agglomerated pollen again after extrusion;
s4, pollen packaging, preparing a transparent centrifugal tube, placing the sieved pollen in the transparent centrifugal tube, placing moisture detection test paper in the transparent centrifugal tube, and sealing the transparent centrifugal tube;
s5, refrigerating pollen, performing low-temperature pretreatment on the transparent centrifugal tube at-25 ℃, then storing in a liquid nitrogen box, and performing shading treatment;
s6, transporting pollen, storing for a period of time, transporting to a female parent planting field, taking out the sealed pollen freezing tube from the liquid nitrogen box at any time according to needs, and slowly thawing at room temperature to recover the pollen;
s7, restoring pollen, wherein when the pollen is in a normal-temperature environment and in a time period between restoration and use of the pollen, water in the pollen is converted from ice into water, and when the pollen absorbs the water, the water content in the pollen can be detected through the water detection test paper;
and S8, pollinating by using a powder sprayer.
Preferably, in S2, after the culture dish containing the pollen is placed in a drying oven, the pollen is dried, the water content of the pollen is tested, when the water content of the pollen is higher than 35%, the pollen is subjected to water absorption treatment by using absorbent paper, and the drying oven is set to be 48-58 ℃ and the drying time is 0.5-0.8h, then the drying oven is set to be 33-42 ℃ and the drying treatment is carried out for 1-2 h;
preferably, in S2, the pollen is dried in a drying oven after the culture dish containing the pollen is placed in the drying oven, and the water content of the pollen is tested;
when the water content of the pollen is 20-35%, setting the drying oven at 42-52 ℃ for 0.5-1h, then setting the drying oven at 33-42 ℃ for drying treatment for 1-2 h;
preferably, in S2, after the culture dish containing the pollen is placed in a drying oven, the pollen is dried, the moisture content of the pollen is tested, when the moisture content of the pollen is lower than 20%, the drying oven is set to be 38-48 ℃ and the drying time is 0.3-0.5h, and then the drying oven is set to be 33-42 ℃ and the drying treatment is carried out for 1-2 h.
Preferably, in S1, when the pollen is loaded into the culture dish, the pollen is spread evenly without any compaction operation, which facilitates the subsequent pollen drying and pollen sieving operations.
Preferably, in S2, grooves are punched on the surface of the pollen during drying, thereby accelerating drying.
Preferably, in S4, one side of the moisture detection test paper is attached to the inner wall of the transparent centrifugal tube. The moisture detection test paper is produced by Wu-Li-Er-Jie biotechnology limited company, can change color when meeting water, and can observe whether the water content of the preserved pollen is increased by naked eyes according to the color change of the moisture detection test paper, so that the time and labor cost for measuring the water content by instrument equipment are reduced.
Preferably, in S3, the mesh number of the screened filter net is 150, which can be more effectively used for screening pollen.
Preferably, in S3, the caked pollen is pressed by a fixing rod, instead of grinding, and the caked pollen is removed by pressing, thereby preventing the activity of the pollen from being damaged.
In summary, due to the adoption of the technology, the invention has the beneficial effects that:
1. according to the invention, the moisture detection test paper is placed in the transparent centrifugal tube for placing the pollen, and whether the water content of the preserved pollen is increased or not can be observed by naked eyes according to the color change of the moisture detection test paper, so that the time for measuring the water content by instrument equipment and the labor cost are reduced.
2. According to the method, the pollen for hybrid seed production of the hot pepper is collected in advance, so that the pollen is reserved at any time, the problems that the flowering period of the hot pepper is not met, and the number and the quality of the pollen do not reach the standard are effectively solved, sufficient pollen is provided for high-quality hybrid seed production of the hot pepper, and the seed production foundation is guaranteed;
3. according to the invention, pollen does not need to be collected in the morning, so that the pollen collecting time is saved, the pollination working state is quickly entered, and the working efficiency of pollination in the day is effectively improved. If the pollen is scattered in rainy days, the pollen scattering time is long, and the problem of waiting for collecting the pollen is well solved.
4. The invention can store and transport the pepper pollen for a long distance and time, has simple and convenient operation and reduces the cost and the technology in the process of storage and transportation.
Drawings
In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings that are required to be used in the embodiments will be briefly described below, it should be understood that the following drawings only illustrate some embodiments of the present invention and therefore should not be considered as limiting the scope, and for those skilled in the art, other related drawings can be obtained according to the drawings without inventive efforts.
FIG. 1 is a flow chart of a method for preserving pepper pollen and rapid cross breeding according to the present invention.
Detailed Description
In order to make the objects, technical solutions and advantages of the embodiments of the present invention more apparent, the technical solutions of the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings of the embodiments of the present invention, and it is obvious that the described embodiments are some, but not all embodiments of the present invention. All other embodiments, which can be obtained by a person skilled in the art without any inventive step based on the embodiments of the present invention, are within the scope of the present invention.
Thus, the following detailed description of the embodiments of the present invention, presented in the figures, is not intended to limit the scope of the invention, as claimed, but is merely representative of selected embodiments of the invention. All other embodiments, which can be obtained by a person skilled in the art without any inventive step based on the embodiments of the present invention, are within the scope of the present invention.
It should be noted that: like reference numbers and letters refer to like items in the following figures, and thus, once an item is defined in one figure, it need not be further defined and explained in subsequent figures.
In the description of the present invention, it is to be understood that the terms "longitudinal", "upper", "lower", "left", "right", "top", "bottom", "inner", "outer", and the like, indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, are used only for convenience in describing the present invention and for simplicity in description, and do not indicate or imply that the referenced devices or elements must have a particular orientation, be constructed and operated in a particular orientation, and thus, are not to be construed as limiting the present invention.
Furthermore, the terms "first", "second" and "first" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defined as "first" or "second" may explicitly or implicitly include one or more of that feature. In the description of the present invention, "a plurality" means two or more unless specifically defined otherwise.
Example 1
A method for preserving pepper pollen and quickly crossbreeding pepper pollen comprises the following steps:
s1, pollen collection, namely collecting pollen with good development of the pepper in the flowering phase, and putting the collected pollen into a culture dish; in S1, when the pollen is put into the culture dish, the pollen is spread evenly, no compacting operation is needed, the pollen agglomeration can be reduced, the heating is convenient, the water content in the pollen is reduced, and the subsequent pollen drying and pollen screening operation is convenient.
S2, drying the pollen, namely putting the culture dish containing the pollen into a drying box, and drying the pollen to ensure that the water content of the pollen is 8-10%; the pollen content is between 8% and 10%, which is convenient for preservation and not easy to inactivate. When drying, the grooves are punched on the surface of the pollen, so that the drying can be accelerated.
S3, screening pollen, taking out the pollen, screening in a sterile room, and screening the agglomerated pollen again after extrusion; the mesh number of the sieved sieve was 150, and the agglomerated pollen was pressed by a fixing bar, not ground. The caking is removed by squeezing, and the activity of pollen is prevented from being damaged.
S4, pollen packaging, preparing a transparent centrifugal tube, placing the sieved pollen in the transparent centrifugal tube, placing moisture detection test paper in the transparent centrifugal tube, and sealing the transparent centrifugal tube; one side of the moisture detection test paper is attached to the inner wall of the transparent centrifugal tube. The moisture detection test paper is produced by Wu-Li-Er-Jie biotechnology limited company, can change color when meeting water, and can observe whether the water content of the preserved pollen is increased by naked eyes according to the color change of the moisture detection test paper, so that the time and labor cost for measuring the water content by instrument equipment are reduced.
S5, refrigerating pollen, pretreating the pollen in the transparent centrifugal tube at a low temperature of-25 ℃, and then storing in a liquid nitrogen box, wherein the basic principle of cell cryopreservation and recovery is slow freezing and fast melting, so the pollen is pretreated, the cell activity can be preserved to the maximum extent, and the pollen inactivation can be prevented by shading treatment.
S6, transporting pollen, storing for a period of time, transporting to a female parent planting field, taking out the sealed pollen freezing tube from the liquid nitrogen box at any time according to needs, and slowly thawing at room temperature to recover the pollen;
s7, restoring pollen, wherein when the pollen is in a normal-temperature environment and in a time period between restoration and use of the pollen, water in the pollen is converted from ice into water, and when the pollen absorbs the water, the water content in the pollen can be detected through the water detection test paper; according to the change of the moisture detection test paper, whether the water content of the preserved pollen is increased or not can be observed by naked eyes, and the time and labor cost for measuring the water content by instrument equipment are reduced.
And S8, pollinating by using a powder sprayer.
In S2, placing the culture dish containing pollen into a drying oven, drying the pollen, testing the water content of the pollen, when the water content of the pollen is higher than 35%, indicating that the surface of the pollen has obvious water stains, performing water absorption treatment on the pollen by using water absorption paper, and primarily reducing the water content of the pollen, wherein the drying oven is set to be 48-58 ℃, so that the drying efficiency can be ensured, the high-temperature inactivation rate of the pollen is reduced, the drying time is 0.5-0.8h, then the water content is reduced, after the water loss protection, the pollen is directly exposed to high temperature, the temperature of the drying oven is reduced to be 33-42 ℃, the inactivation of the pollen is prevented, the drying treatment is performed for 1-2h, the treatment time is less than 1h, the drying time does not meet the requirement, and the water content reduction speed is obviously reduced for more than 2 h.
Example 2
A method for preserving pepper pollen and quickly crossbreeding pepper pollen comprises the following steps:
s1, pollen collection, namely collecting pollen with good development of the pepper in the flowering phase, and putting the collected pollen into a culture dish; in S1, when the pollen is put into the culture dish, the pollen is spread evenly, no compacting operation is needed, the pollen agglomeration can be reduced, the heating is convenient, the water content in the pollen is reduced, and the subsequent pollen drying and pollen screening operation is convenient.
S2, drying the pollen, namely putting the culture dish containing the pollen into a drying box, and drying the pollen to ensure that the water content of the pollen is 8-10%; the pollen content is between 8% and 10%, which is convenient for preservation and not easy to inactivate. When drying, the grooves are punched on the surface of the pollen, so that the drying can be accelerated.
S3, screening pollen, taking out the pollen, screening in a sterile room, and screening the agglomerated pollen again after extrusion; the mesh number of the sieved sieve was 150, and the agglomerated pollen was pressed by a fixing bar, not ground. The caking is removed by squeezing, and the activity of pollen is prevented from being damaged.
S4, pollen packaging, preparing a transparent centrifugal tube, placing the sieved pollen in the transparent centrifugal tube, placing moisture detection test paper in the transparent centrifugal tube, and sealing the transparent centrifugal tube; one side of the moisture detection test paper is attached to the inner wall of the transparent centrifugal tube. The moisture detection test paper is produced by Wu-Li-Er-Jie biotechnology limited company, can change color when meeting water, and can observe whether the water content of the preserved pollen is increased by naked eyes according to the color change of the moisture detection test paper, so that the time and labor cost for measuring the water content by instrument equipment are reduced.
S5, refrigerating pollen, pretreating the pollen in the transparent centrifugal tube at a low temperature of-25 ℃, and then storing in a liquid nitrogen box, wherein the basic principle of cell cryopreservation and recovery is slow freezing and fast melting, so the pollen is pretreated, the cell activity can be preserved to the maximum extent, and the pollen inactivation can be prevented by shading treatment.
S6, transporting pollen, storing for a period of time, transporting to a female parent planting field, taking out the sealed pollen freezing tube from the liquid nitrogen box at any time according to needs, and slowly thawing at room temperature to recover the pollen;
s7, restoring pollen, wherein when the pollen is in a normal-temperature environment and in a time period between restoration and use of the pollen, water in the pollen is converted from ice into water, and when the pollen absorbs the water, the water content in the pollen can be detected through the water detection test paper; according to the change of the moisture detection test paper, whether the water content of the preserved pollen is increased or not can be observed by naked eyes, and the time and labor cost for measuring the water content by instrument equipment are reduced.
And S8, pollinating by using a powder sprayer.
In S2, placing the culture dish filled with the pollen into a drying box, drying the pollen, and firstly testing the water content of the pollen; when the water content of the pollen is 20-35%, the drying oven is set to 42-52 ℃ and the drying time is 0.5-1h, so that the drying efficiency can be ensured, and the high-temperature inactivation rate of the pollen is reduced. And then setting the temperature of the drying box to be 33-42 ℃, drying for 1-2h, wherein the drying time is less than 1h, the drying time cannot meet the requirement, and the drying time is more than 2h, so that the moisture content reduction speed is obviously slowed down.
Example 3
A method for preserving pepper pollen and quickly crossbreeding pepper pollen comprises the following steps:
s1, pollen collection, namely collecting pollen with good development of the pepper in the flowering phase, and putting the collected pollen into a culture dish; in S1, when the pollen is put into the culture dish, the pollen is spread evenly, no compacting operation is needed, the pollen agglomeration can be reduced, the heating is convenient, the water content in the pollen is reduced, and the subsequent pollen drying and pollen screening operation is convenient.
S2, drying the pollen, namely putting the culture dish containing the pollen into a drying box, and drying the pollen to ensure that the water content of the pollen is 8-10%; the pollen content is between 8% and 10%, which is convenient for preservation and not easy to inactivate. When drying, the grooves are punched on the surface of the pollen, so that the drying can be accelerated.
S3, screening pollen, taking out the pollen, screening in a sterile room, and screening the agglomerated pollen again after extrusion; the mesh number of the sieved sieve was 150, and the agglomerated pollen was pressed by a fixing bar, not ground. The caking is removed by squeezing, and the activity of pollen is prevented from being damaged.
S4, pollen packaging, preparing a transparent centrifugal tube, placing the sieved pollen in the transparent centrifugal tube, placing moisture detection test paper in the transparent centrifugal tube, and sealing the transparent centrifugal tube; one side of the moisture detection test paper is attached to the inner wall of the transparent centrifugal tube. The moisture detection test paper is produced by Wu-Li-Er-Jie biotechnology limited company, can change color when meeting water, and can observe whether the water content of the preserved pollen is increased by naked eyes according to the color change of the moisture detection test paper, so that the time and labor cost for measuring the water content by instrument equipment are reduced.
S5, refrigerating pollen, pretreating the pollen in the transparent centrifugal tube at a low temperature of-25 ℃, and then storing in a liquid nitrogen box, wherein the basic principle of cell cryopreservation and recovery is slow freezing and fast melting, so the pollen is pretreated, the cell activity can be preserved to the maximum extent, and the pollen inactivation can be prevented by shading treatment.
S6, transporting pollen, storing for a period of time, transporting to a female parent planting field, taking out the sealed pollen freezing tube from the liquid nitrogen box at any time according to needs, and slowly thawing at room temperature to recover the pollen;
s7, restoring pollen, wherein when the pollen is in a normal-temperature environment and in a time period between restoration and use of the pollen, water in the pollen is converted from ice into water, and when the pollen absorbs the water, the water content in the pollen can be detected through the water detection test paper; according to the change of the moisture detection test paper, whether the water content of the preserved pollen is increased or not can be observed by naked eyes, and the time and labor cost for measuring the water content by instrument equipment are reduced.
And S8, pollinating by using a powder sprayer.
In S2, placing the culture dish containing the pollen into a drying oven, drying the pollen, testing the water content of the pollen, setting the drying oven to be 38-48 ℃ when the water content of the pollen is lower than 20%, setting the drying time to be 0.3-0.5h, setting the drying oven to be 33-42 ℃, drying for 1-2h, setting the drying time to be lower than 1h, making the drying time not meet the requirement, and setting the drying time to be higher than 2h, wherein the reduction speed of the water content is obviously reduced.
The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention, and various modifications and changes may be made by those skilled in the art. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.
Claims (9)
1. A pepper pollen preservation and rapid cross breeding method is characterized by comprising the following steps:
s1, pollen collection, namely collecting pollen with good development of the pepper in the flowering phase, and putting the collected pollen into a culture dish;
s2, drying the pollen, namely putting the culture dish containing the pollen into a drying box, and drying the pollen to ensure that the water content of the pollen is 8-10%;
s3, screening pollen, taking out the pollen, screening in a sterile room, and screening the agglomerated pollen again after extrusion;
s4, pollen packaging, preparing a transparent centrifugal tube, placing the sieved pollen in the transparent centrifugal tube, placing moisture detection test paper in the transparent centrifugal tube, and sealing the transparent centrifugal tube;
s5, refrigerating pollen, performing low-temperature pretreatment on the transparent centrifugal tube at-25 ℃, then storing in a liquid nitrogen box, and performing shading treatment;
s6, transporting pollen, storing for a period of time, transporting to a female parent planting field, taking out the sealed pollen freezing tube from the liquid nitrogen box at any time according to needs, and slowly thawing at room temperature to recover the pollen;
s7, restoring pollen, wherein when the pollen is in a normal-temperature environment and in a time period between restoration and use of the pollen, water in the pollen is converted from ice into water, and when the pollen absorbs the water, the water content in the pollen can be detected through the water detection test paper;
and S8, pollinating by using a powder sprayer.
2. The method for pepper pollen preservation and rapid cross breeding as claimed in claim 1, wherein: in S2, after the culture dish containing the pollen is placed in a drying oven, the pollen is dried, the water content of the pollen is tested, when the water content of the pollen is higher than 35%, the pollen is subjected to water absorption treatment by using water absorption paper, the drying oven is set to be 48-58 ℃ and the drying time is 0.5-0.8h, and then the drying oven is set to be 33-42 ℃ and the drying treatment is carried out for 1-2 h.
3. The method for pepper pollen preservation and rapid cross breeding as claimed in claim 1, wherein: in S2, placing the culture dish filled with the pollen into a drying box, drying the pollen, and firstly testing the water content of the pollen;
when the water content of the pollen is 20-35%, setting the drying oven at 42-52 ℃ for 0.5-1h, then setting the drying oven at 33-42 ℃ for drying treatment for 1-2 h.
4. The method for pepper pollen preservation and rapid cross breeding as claimed in claim 1, wherein: in S2, the culture dish containing the pollen is placed in a drying oven, the pollen is dried, the moisture content of the pollen is tested, when the moisture content of the pollen is lower than 20%, the drying oven is set to be 38-48 ℃ and the drying time is 0.3-0.5h, then the drying oven is set to be 33-42 ℃ and the drying treatment is carried out for 1-2 h.
5. The method for pepper pollen preservation and rapid cross breeding as claimed in claim 1, wherein: in S1, when the pollen is loaded into the culture dish, the pollen is spread out evenly without any compaction operation.
6. The method for pepper pollen preservation and rapid cross breeding as claimed in claim 1, wherein: in S2, the grooves are punched on the pollen surface during drying, thereby accelerating the drying.
7. The method for pepper pollen preservation and rapid cross breeding as claimed in claim 1, wherein: in S4, one side of the moisture detection test paper was attached to the inner wall of the transparent centrifugal tube.
8. The method for pepper pollen preservation and rapid cross breeding as claimed in claim 1, wherein: in S3, the mesh number of the sieve to be sieved is 150.
9. The method for pepper pollen preservation and rapid cross breeding as claimed in claim 1, wherein: in S3, the caked pollen is pressed by a fixing bar, not ground.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114451405A (en) * | 2022-01-14 | 2022-05-10 | 遵义市农业科学研究院 | Preservation method of pepper pollen |
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| CN102415370A (en) * | 2011-08-18 | 2012-04-18 | 湖南省蔬菜研究所 | Ultralow temperature preservation method for capsicum pollens |
| CN103392590A (en) * | 2013-08-06 | 2013-11-20 | 镇江市蔬菜研究所 | Capsicum injection-pollination method |
| CN103719076A (en) * | 2013-12-25 | 2014-04-16 | 广东省农业科学院果树研究所 | Litchi pollen collecting and storing method |
| CN108477145A (en) * | 2018-04-27 | 2018-09-04 | 湖南省蔬菜研究所 | The simple storage practice of capsicum pollens |
| CN208144317U (en) * | 2018-04-27 | 2018-11-27 | 湖南省蔬菜研究所 | The simple stowage arrangement of capsicum pollens |
| CN112262843A (en) * | 2020-10-12 | 2021-01-26 | 杭州市农业科学研究院 | Preservation method and preservation device for pepper pollen |
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2021
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102415370A (en) * | 2011-08-18 | 2012-04-18 | 湖南省蔬菜研究所 | Ultralow temperature preservation method for capsicum pollens |
| CN103392590A (en) * | 2013-08-06 | 2013-11-20 | 镇江市蔬菜研究所 | Capsicum injection-pollination method |
| CN103719076A (en) * | 2013-12-25 | 2014-04-16 | 广东省农业科学院果树研究所 | Litchi pollen collecting and storing method |
| CN108477145A (en) * | 2018-04-27 | 2018-09-04 | 湖南省蔬菜研究所 | The simple storage practice of capsicum pollens |
| CN208144317U (en) * | 2018-04-27 | 2018-11-27 | 湖南省蔬菜研究所 | The simple stowage arrangement of capsicum pollens |
| CN112262843A (en) * | 2020-10-12 | 2021-01-26 | 杭州市农业科学研究院 | Preservation method and preservation device for pepper pollen |
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| CN114451405A (en) * | 2022-01-14 | 2022-05-10 | 遵义市农业科学研究院 | Preservation method of pepper pollen |
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