CN103238589A - Storage method of lily powder - Google Patents
Storage method of lily powder Download PDFInfo
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- CN103238589A CN103238589A CN2013101736766A CN201310173676A CN103238589A CN 103238589 A CN103238589 A CN 103238589A CN 2013101736766 A CN2013101736766 A CN 2013101736766A CN 201310173676 A CN201310173676 A CN 201310173676A CN 103238589 A CN103238589 A CN 103238589A
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Abstract
The invention discloses a storage method of lily powder. The storage method comprises the following steps of: (1), preparing a constant-humidity salt solution-sodium nitrite saturated solution; (2), adding the prepared solution to the bottom of a dryer until the liquid surface is 1-2cm away from the porcelain plate of the dryer, sealing for later use, wherein the relative humidity is 60%-70%; (3), filling collected pollen to a gelatin hollow capsule, covering the capsule cover, and puncturing a pore at each of the two ends of the capsule respectively for facilitating gas exchange; (4), placing the capsule filled with the pollen to an envelope or a paper bag, using a paper clip for sealing and placing on the porcelain plate of the dryer, wherein the sealing by a paper clip aims at facilitating gas exchange; and (5), sealing the dryer and placing to a refrigerator of 5 DEG C for storage for a long time. The storage method of the lily powder is simple and easy to implement; and the service life of the lily powder stored by the method can achieve one year or longer.
Description
Technical field
The present invention relates to a kind of storage practice of lilium pollen.
Background technology
Pollen is the microspore of flowering plant, produces the male gametophyte that contains three haploid nucleuses during sprouting, and whole hereditary information that it is comprising this plant are important materials that plant hybridization breeding and germ plasm resource are preserved.Pollen viability refers to the ability of survival, growth, sprouting or growth that pollen has, is one of key factor of crossbreeding success.The pollen life-span be pollen under certain condition pollen keep the blodynamic time limit.
Lily (Lilium spp.) is important flowering bulb.The natural life-span of lilium pollen is short, and crossbreeding regular meeting often runs into problems such as this flowering asynchronism of father and mother and remote hybridization, and the breeder need address the above problem by pollen storage; In addition, pollen storage also is the important technology of the preservation of lily germ plasm resource.
Lily is of a great variety, life-span has only 1-3 week to ripe lilium pollen under field conditions (factors), existing storage practice is that the pollen of lily is preserved under low temperature or condition of ultralow temperature, prolong the life-span of pollen by the control temperature, specifically being the lilium pollen that will gather preserves under cryogenic conditions such as constant 5 ℃ ,-18 ℃, or preserves under the cryogenic conditions of ultralow temperature-80 ℃ and-196 ℃ (liquid nitrogen).There is following defective in above-mentioned storage practice:
1, under 5 ℃ ,-18 ℃ equitemperatures, the time that the lilium pollen life-span keeps is shorter, can only keep 30-80 days, and the length instability that keeps of pollen life-span, can not satisfy the breeder to the requirement of anniversary of lilium pollen or more long-term storage.
2, preserve under the condition of ultralow temperature, need ultra low temperature freezer or liquid nitrogen, the cost height of preservation must progressively be revived the pollen of storage under different temperatures during use successively, and the program complexity is loaded down with trivial details.
Summary of the invention
The storage practice that the purpose of this invention is to provide a kind of lilium pollen, this method can make the shelf-life of lilium pollen just can stably extend to more than 1 year under 5 ℃ of temperature, and preserving type is simple and practical.
The applicant finds after deliberation, the length in lilium pollen life-span is except being subjected to self hereditary capacity influences, environmental condition especially temperature and humidity has a significant impact the vitality of pollen, and wherein the influence of ambient humidity is bigger relatively, and humidity is crossed and exceeded the low shelf-life that all can have a strong impact on pollen.Under 5 ℃ of conditions of temperature, the shelf-life of lilium pollen was the longest when relative moisture was 60-70%, can reach more than 1 year.
Based on above-mentioned research, the present invention is by controlling the life-span that two material impact factors of temperature and humidity prolong lilium pollen simultaneously, and its concrete technical scheme is as follows:
1) configuration of constant humidity salting liquid-natrium nitrosum saturated solution;
2) the sodium nitrate saturated solution that configures is joined in drier bottom, keep the 1-2cm spacing apart from the drier porcelain plate to liquid level, the sealing back is standby;
3) pollen of collecting is packed in the gelatin Capsules, cover capsule lid after, respectively sting an aperture at the capsule two ends, exchange in order to gas;
4) will pack into the capsule of pollen is put into envelope or paper bag, is placed on the drier porcelain plate after sealing with clip, and the purpose of sealing with clip is to be beneficial to the gas exchange;
5) put into after drier is airtight that 5 ℃ of refrigerators are medium-term and long-term to be preserved.
In the step 1), in order to guarantee the constant humidity effect, the natrium nitrosum saturated solution is prepared with distilled water or pure water, and the natrium nitrosum that adopts is pure for analyzing, and the natrium nitrosum saturated solution of preparing should have the natrium nitrosum more than 30% to separate out when temperature drops to 5 ℃;
Step 2) in, the addition of sodium nitrate saturated solution is fixed according to the specification size of drier, and the drier specification is decided according to the size of refrigerating box and the pollen amount of needs storage, and can select internal diameter for use is the different size drier of 100mm-180mm.Making liquid level keep the purpose of 1-2cm distance apart from porcelain plate after the sodium nitrate saturated solution adds is that liquid influences the porcelain plate top when preventing from operating.
In the step 3), have higher vitality for guaranteeing the pollen of gathering, described pollen is the pollen of blooming and gathering the same day, collects lilium pollen with banister brush during collection; The preferred medical gelatin empty hard capsule of the capsule that adopts, for not influencing the gas permeability of capsule, each capsule charge weight is no more than 20mg, and the small aperture at capsule two ends is 0.5-0.7mm, and the too small gas that is unfavorable for exchanges, and crosses conference and causes pollen to release.
The present invention has following advantage and progress:
1, temperature control adopts common cold storage refrigerator to get final product, and is simple and easy to do;
2, natrium nitrosum saturated solution constant humidity, the relative moisture of this solution is acted upon by temperature changes little, therefore relative moisture that can constant solution top air, keep the three-phase thermodynamical equilibrium, when temperature during-5 ℃ to 30 ℃ range the relative moisture in the drier be 63-66%, so constant humidity effect stability;
3, adopt gelatine capsule quantitatively to deposit pollen, can guarantee uniformity and the stability of duration of storage pollen week environment condition, and can make a return journey by the actual demand amount during the taking of pollen, convenient and simple.
4, this method can not only keep temperature constant, and the energy constant humidity, and under 5 ℃ of temperature and 60-70% relative moisture, the lilium pollen life-span can keep more than 1 year.
Embodiment
1) configuration of constant humidity salting liquid Asia-sodium nitrate saturated solution
Get the 100ml pure water, middle adding natrium nitrosum 110g, heating for dissolving.
2) getting 200ml, to configure that above-mentioned solution joins at internal diameter be the glass drier bottom (GB/T15723-1995) of 100mm, to liquid level be 1-2cm apart from the drier porcelain plate, the sealing back is standby;
3) select the lily flower of blooming the same day, banister brush is gathered pollen, with the pollen of the gathering 00# medical gelatin empty hard capsule (Chinese Medicine packing association criterion YBX-2000-2007) of packing into, each capsule charge weight is no more than 20mg, after covering capsule lid, respectively sting an aperture at the capsule two ends with pin, aperture 0.5-0.7mm exchanges in order to gas.
4) will pack into the capsule of pollen is put into envelope or paper bag, is placed on the drier porcelain plate after sealing with clip.
5) put into after the glass drier is airtight that 5 ℃ of refrigerators are medium-term and long-term to be preserved.
Adopt this method with the kind ' lanzhou lily ' (L ilium davi dii var.unicolor) of Asia lily system, ' lilium regale wilson ' (L ilium regale), the kind ' Siberia ' of oriental hybrid lily system, ' rope freshwater mussel ', lilium longiflorum is the pollen storage 1 year of kind 5 kinds such as ' Juliets Si ', germination rate is respectively 55%, 65% after testing, 61%, 60%, 58%, 68%, all can satisfy breeder's demand.
Claims (4)
1. the storage practice of a lilium pollen is characterized in that comprising the steps:
1) configuration of constant humidity salting liquid-natrium nitrosum saturated solution;
2) solution that configures is joined in drier bottom, keep the 1-2cm spacing apart from the drier porcelain plate to liquid level, seal standbyly, relative moisture is 60-70%;
3) pollen of collecting is packed in the gelatin Capsules, cover capsule lid after, respectively sting an aperture at the capsule two ends, exchange in order to gas;
4) will pack into the capsule of pollen is put into envelope or paper bag, is placed on the drier porcelain plate after sealing with clip, and the purpose of sealing with clip is to be beneficial to the gas exchange;
5) put into after drier is airtight that 5 ℃ of refrigerators are medium-term and long-term to be preserved.
2. the storage practice of lilium pollen as claimed in claim 1, it is characterized in that the natrium nitrosum saturated solution is prepared with distilled water or pure water in the step 1), the natrium nitrosum that adopts is pure for analyzing, and the natrium nitrosum saturated solution of preparing should have the natrium nitrosum more than 30% to separate out when temperature drops to 5 ℃.
3. the storage practice of lilium pollen as claimed in claim 1 is characterized in that in the step 3), and pollen is the pollen of blooming and gathering the same day, collects lilium pollen with banister brush during collection.
4. the storage practice of lilium pollen as claimed in claim 1 is characterized in that in the step 3), and the capsule that adopts is the medical gelatin empty hard capsule, and each capsule charge weight is no more than 20mg, and the small aperture at capsule two ends is 0.5-0.7mm.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310173676.6A CN103238589B (en) | 2013-05-10 | 2013-05-10 | Storage method of lily powder |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201310173676.6A CN103238589B (en) | 2013-05-10 | 2013-05-10 | Storage method of lily powder |
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| CN103238589A true CN103238589A (en) | 2013-08-14 |
| CN103238589B CN103238589B (en) | 2014-12-31 |
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| CN201310173676.6A Expired - Fee Related CN103238589B (en) | 2013-05-10 | 2013-05-10 | Storage method of lily powder |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105104362A (en) * | 2015-08-13 | 2015-12-02 | 广西壮族自治区中国科学院广西植物研究所 | Simple long-term storage preservation method for pitaya pollen |
| CN109287623A (en) * | 2018-10-18 | 2019-02-01 | 南京林业大学 | A kind of purple U.S. pomegranate pollen storage practice |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN101040600A (en) * | 2007-04-13 | 2007-09-26 | 浙江省农业科学院 | Method of crossbreeding and quick propagating the lilium formolongi seed and its parents |
| JP2007325533A (en) * | 2006-06-07 | 2007-12-20 | Kagoshima Univ | Method for predicting flower color |
| KR20120119933A (en) * | 2011-04-21 | 2012-11-01 | 강원대학교산학협력단 | Mouthcare and breath freshener containing natural medicinal stuffs and propolis |
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2013
- 2013-05-10 CN CN201310173676.6A patent/CN103238589B/en not_active Expired - Fee Related
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2007325533A (en) * | 2006-06-07 | 2007-12-20 | Kagoshima Univ | Method for predicting flower color |
| CN101040600A (en) * | 2007-04-13 | 2007-09-26 | 浙江省农业科学院 | Method of crossbreeding and quick propagating the lilium formolongi seed and its parents |
| KR20120119933A (en) * | 2011-04-21 | 2012-11-01 | 강원대학교산학협력단 | Mouthcare and breath freshener containing natural medicinal stuffs and propolis |
Non-Patent Citations (4)
| Title |
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| 周蕴薇 等: "百合花粉生活力及贮藏特性1’", 《东北林业大学学报》 * |
| 尹恒 等: "麝香百合花粉发芽适宜培养基及其贮藏研究", 《长江大学学报（自科版）农学卷》 * |
| 殷勇高 等: "两种盐溶液除湿器的建模与对比", 《化工学报》 * |
| 赵兴华 等: "百合花粉生活力测定和贮藏方法研究", 《北方园艺》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105104362A (en) * | 2015-08-13 | 2015-12-02 | 广西壮族自治区中国科学院广西植物研究所 | Simple long-term storage preservation method for pitaya pollen |
| CN109287623A (en) * | 2018-10-18 | 2019-02-01 | 南京林业大学 | A kind of purple U.S. pomegranate pollen storage practice |
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| CN103238589B (en) | 2014-12-31 |
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