CN113249199B - Sample adding unit and cell extraction instrument - Google Patents
Sample adding unit and cell extraction instrument Download PDFInfo
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- CN113249199B CN113249199B CN202110611292.2A CN202110611292A CN113249199B CN 113249199 B CN113249199 B CN 113249199B CN 202110611292 A CN202110611292 A CN 202110611292A CN 113249199 B CN113249199 B CN 113249199B
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- 238000000605 extraction Methods 0.000 title claims abstract description 16
- 238000007789 sealing Methods 0.000 claims abstract description 147
- 239000007788 liquid Substances 0.000 claims abstract description 89
- 238000002347 injection Methods 0.000 claims description 47
- 239000007924 injection Substances 0.000 claims description 47
- 238000011068 loading method Methods 0.000 claims description 30
- 238000011049 filling Methods 0.000 claims description 12
- 239000011248 coating agent Substances 0.000 claims description 4
- 238000000576 coating method Methods 0.000 claims description 4
- 238000004140 cleaning Methods 0.000 abstract description 19
- 239000003344 environmental pollutant Substances 0.000 abstract description 5
- 231100000719 pollutant Toxicity 0.000 abstract description 5
- 230000000694 effects Effects 0.000 abstract description 4
- 238000005070 sampling Methods 0.000 description 31
- 210000004027 cell Anatomy 0.000 description 12
- 238000000034 method Methods 0.000 description 8
- 239000000243 solution Substances 0.000 description 8
- 238000005516 engineering process Methods 0.000 description 5
- 238000010790 dilution Methods 0.000 description 3
- 239000012895 dilution Substances 0.000 description 3
- 238000000926 separation method Methods 0.000 description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 3
- 238000010586 diagram Methods 0.000 description 2
- 239000003085 diluting agent Substances 0.000 description 2
- 238000001471 micro-filtration Methods 0.000 description 2
- 238000005192 partition Methods 0.000 description 2
- 208000005443 Circulating Neoplastic Cells Diseases 0.000 description 1
- 238000004166 bioassay Methods 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 238000003320 cell separation method Methods 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 210000002615 epidermis Anatomy 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 239000012634 fragment Substances 0.000 description 1
- 239000003292 glue Substances 0.000 description 1
- 210000000265 leukocyte Anatomy 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 210000000582 semen Anatomy 0.000 description 1
- -1 semen Substances 0.000 description 1
- 238000004544 sputter deposition Methods 0.000 description 1
- 229910001220 stainless steel Inorganic materials 0.000 description 1
- 239000010935 stainless steel Substances 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 210000001519 tissue Anatomy 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/38—Caps; Covers; Plugs; Pouring means
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B08—CLEANING
- B08B—CLEANING IN GENERAL; PREVENTION OF FOULING IN GENERAL
- B08B9/00—Cleaning hollow articles by methods or apparatus specially adapted thereto
- B08B9/02—Cleaning pipes or tubes or systems of pipes or tubes
- B08B9/023—Cleaning the external surface
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B08—CLEANING
- B08B—CLEANING IN GENERAL; PREVENTION OF FOULING IN GENERAL
- B08B9/00—Cleaning hollow articles by methods or apparatus specially adapted thereto
- B08B9/02—Cleaning pipes or tubes or systems of pipes or tubes
- B08B9/027—Cleaning the internal surfaces; Removal of blockages
- B08B9/032—Cleaning the internal surfaces; Removal of blockages by the mechanical action of a moving fluid, e.g. by flushing
- B08B9/0321—Cleaning the internal surfaces; Removal of blockages by the mechanical action of a moving fluid, e.g. by flushing using pressurised, pulsating or purging fluid
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
- C12M33/04—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by injection or suction, e.g. using pipettes, syringes, needles
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M37/00—Means for sterilizing, maintaining sterile conditions or avoiding chemical or biological contamination
- C12M37/04—Seals
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M39/00—Means for cleaning the apparatus or avoiding unwanted deposits of microorganisms
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Biomedical Technology (AREA)
- Sustainable Development (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Molecular Biology (AREA)
- Mechanical Engineering (AREA)
- Clinical Laboratory Science (AREA)
- Sampling And Sample Adjustment (AREA)
- Automatic Analysis And Handling Materials Therefor (AREA)
Abstract
The invention provides a sample adding unit and a cell extraction instrument, which relate to the technical field of cell extraction and comprise a sample adding tube, a needle outer sleeve, a sealing mechanism and a sample adding needle; when the inner channel of the sample adding needle needs to be cleaned, the outer sleeve of the needle is utilized to drive the sample adding needle to be in sealing connection with the sealing channel, so that liquid in the sample adding tube can only enter the inner channel of the sample adding needle, when the outer side wall of the sample adding needle needs to be cleaned, the outer sleeve of the needle is utilized to drive the sample adding needle to be separated from the sealing channel in a sealing mode, so that the liquid in the sample adding tube can enter the outer sleeve of the needle along the sealing channel, cleaning liquid can flow along the outer side wall of the sample adding needle, the liquid can flow along the outer side wall of the whole sample adding needle by utilizing the guide hole, the effect of cleaning the inner wall and the outer wall of the sample adding needle is achieved, the technical problem that the outer wall pollutants of the sample adding needle can pollute the follow-up sample extraction in the prior art is relieved.
Description
Technical Field
The invention relates to the technical field of cell extraction, in particular to a sample adding unit and a cell extraction instrument.
Background
In the prior art, the studies on the cell separation method of the mixed biological assay mainly include: the method can be used for directionally capturing the target cells with high precision, but has the problems of complex sample pretreatment, expensive equipment consumable, complex operation and difficult popularization; in addition, as a separation technology, the microfiltration technology plays an important role in the medical fields of leukocyte filtration separation, circulating tumor cell separation and capture and the like along with the development of materials and preparation technology, especially continuous progress of precision processing technology.
In the prior art, the microfiltration technology is limited to manual operation, consumes manpower and is easy to pollute, in the cell extraction process of cell extraction equipment, a sample (such as epidermis fragments, semen, blood, residual samples of human tissues and other amorphous samples) is placed in a sample cup, and is dissolved by injecting diluent, in the prior art, an injection needle is usually adopted to extend into a cup space from an opening at the upper end of the sample cup, the injection needle injects liquid by utilizing a needle hole at the end, and the filtered sample cup needs to be cleaned and filtered again, so that the sample loss is reduced and the cell sample is collected maximally. However, in this process, since the injection has a certain fluid impact force, the vacuum at the end of the injection needle will jet the diluent vertically downward, and thus a large amount of sputtering will be inevitably generated, and the sample-adding needle will be polluted, so that it is necessary to clean the needle, and the subsequent sample will be prevented from being polluted in the next injection process.
However, in the prior art, in the cleaning process of the sample adding needle, after the sample adding needle finishes liquid injection, the sample adding needle is moved to the cleaning position and then the cleaning liquid starts to be injected, but in the process, the liquid only can clean the sample adding needle and the internal passage of the needle tube, the outer wall of the sample adding needle cannot be cleaned, and then when the liquid is injected by the sample adding needle, the possibility that the subsequently injected liquid contacts with the pollutant on the outer wall of the sample adding needle and further pollutes the subsequently extracted sample still exists.
Disclosure of Invention
The invention aims to provide a sample adding unit and a cell extraction instrument, which are used for solving the technical problems that in the prior art, a sample adding needle can only be cleaned and the internal passage of a needle tube can not be cleaned in the cleaning process, and the possibility that the outer wall pollutant of the next sample injection and adding needle pollutes the subsequent sample extraction exists.
The invention provides a sample adding unit, which comprises: the device comprises a sample adding tube, a needle outer sleeve, a sealing mechanism and a sample adding needle;
the needle-filling tube is provided with a liquid filling channel, the needle-coating tube is sleeved outside the liquid filling tube, the sealing mechanism is positioned at one end of the liquid filling tube, which is close to the needle-coating tube, and is connected with the liquid filling tube, and the sealing mechanism is provided with a sealing channel communicated with the liquid filling channel;
the utility model discloses a sample injection needle, including the outer tube of needle, the outer tube of needle keep away from the one end of sampling tube with the sample injection needle is connected, the sample injection needle stretches into to inside the outer tube of needle, annotate the liquid passageway be used for to the direction of sample injection needle carries liquid, the outer tube of needle is used for driving the sample injection needle for the sample injection tube removes, so that the sample injection needle with sealed passageway is connected or is separated, so that the liquid that annotate the liquid passageway and carry enters into the inner channel of sample injection needle or along the lateral wall of sample injection needle flows, sample injection needle with be provided with between the outer tube of needle be used for with liquid along the lateral wall flow water conservancy diversion hole of sample injection needle.
In a preferred embodiment of the invention, the sealing mechanism comprises a sealing plug cover;
the sealing channel is positioned at the center of the sealing plug cover, the sealing plug cover is fixedly connected with the end part of the sample adding pipe along the circumferential direction of the sample adding pipe, and the sample adding needle is used for being in sealing connection with the sealing plug cover.
In a preferred embodiment of the invention, the sealing channel is in an inverted cone shape, the inner diameter of the sealing channel is smaller than the diameter of the sample adding needle, and the sample adding needle extends into the sample adding tube along the inclined surface of the sealing channel.
In a preferred embodiment of the invention, the sealing plug cover is provided with a stepped groove, the sealing plug cover is clamped at the end part of the sample adding pipe through the stepped groove, and the sealing plug cover is adhered with the sample adding pipe through the stepped groove.
In a preferred embodiment of the invention, the sealing mechanism further comprises a fixed disk;
the fixed disk is sleeved outside the sample adding needle along the circumferential direction of the sample adding needle, the fixed disk and one end of the sample adding needle extending into the outer needle sleeve are connected, the diameter of the fixed disk is smaller than the inner diameter of the outer needle sleeve, the diameter of the fixed disk is larger than the inner diameter of the sealing channel, and the fixed disk is used for being abutted to the sealing plug cover so that the sample adding needle is in sealing connection with the sealing channel.
In a preferred embodiment of the invention, the device further comprises a deflector;
the guide disc is located inside the needle outer sleeve, the guide disc with the inner wall connection of needle outer sleeve, the guide disc is located the fixed disk is kept away from one side of sealing plug lid, the center of guide disc is provided with and is used for the guide hole that the application of sample needle runs through, the internal diameter of guide hole is greater than the external diameter of application of sample needle, the guide disc is used for right the seal passageway with the liquid that the clearance was flowed out between the fixed disk carries out the water conservancy diversion, so that liquid is along the lateral wall surface of application of sample needle flows.
In a preferred embodiment of the present invention, the baffle plate is disposed obliquely along one end close to the fixed plate to the other end, and the liquid flowing out from the gap between the sealing channel and the fixed plate flows along the inclined surface of the baffle plate.
In a preferred embodiment of the invention, the outer needle cannula is connected with the sampling tube through threads, and the outer needle cannula is attached and sealed with the end part of the sealing plug cover.
In a preferred embodiment of the present invention, a plurality of flow guiding holes are provided, and the plurality of flow guiding holes are arranged at intervals along the circumferential direction of the sample adding needle, and the flow guiding holes are used for flowing the liquid in the outer sleeve of the needle along the outer side wall of the sample adding needle.
The invention provides a cell extraction instrument which comprises the sample adding unit.
The invention provides a sample adding unit, which comprises: the device comprises a sample adding tube, a needle outer sleeve, a sealing mechanism and a sample adding needle; the needle outer sleeve is sleeved outside the sample adding tube, the sealing mechanism is positioned at one end of the sample adding tube close to the needle outer sleeve, the sealing mechanism is connected with the sample adding tube, and the sealing mechanism is provided with a sealing channel communicated with the liquid injecting channel; the end of the needle outer sleeve, which is far away from the sampling tube, is connected with the sampling needle, the sampling needle extends into the needle outer sleeve, the liquid injection channel is used for conveying liquid to the direction of the sampling needle, the needle outer sleeve is used for driving the sampling needle to move relative to the sampling tube so as to connect or separate the sampling needle from the sealing channel, the liquid conveyed by the liquid injection channel enters into the internal channel of the sampling needle or flows along the outer side wall of the sampling needle, and a flow guide hole for flowing the liquid along the outer side wall of the sampling needle is arranged between the sampling needle and the needle outer sleeve; the utility model discloses a liquid sample adding needle, including the outer wall of needle outer tube, the inner channel of needle outer tube, when needs to wash the inner channel of needle, utilize the needle outer tube to drive the inner channel of needle and seal the passageway when the needle outer tube, make the liquid of needle can enter into the needle outer tube inside along the seal passageway, and the washing liquid can flow along the outer wall of needle, utilize the water conservancy diversion hole can make the liquid flow along the outer lateral wall of whole needle, the effect of all carrying out abluent inside and outside the needle of sample adding has been realized, the needle that has in the prior art can only wash the needle and needle tubing inner passage of sample adding in the cleaning process, can't wash the outer wall of needle, the outer wall pollutant of the needle of the next time annotating liquid sample adding can pollute the technical problem of follow-up extraction sample possibility.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings that are needed in the description of the embodiments or the prior art will be briefly described, and it is obvious that the drawings in the description below are some embodiments of the present invention, and other drawings can be obtained according to the drawings without inventive effort for a person skilled in the art.
FIG. 1 is a schematic diagram of the overall structure of a sample loading unit according to an embodiment of the present invention;
FIG. 2 is a schematic diagram of the overall structure of a sealing mechanism of a sample loading unit provided by an embodiment of the present invention with a fixed disk;
fig. 3 is a schematic structural view of a connection position between an outer needle sleeve and a sample injection needle of a sample injection unit according to an embodiment of the present invention.
Icon: 100-sampling tube; 101-a liquid injection channel; 200-a needle outer sleeve; 201-a deflector hole; 300-sealing mechanism; 301-sealing the channel; 302-sealing the plug cover; 303-a fixed disk; 400-sample adding needle; 500-a deflector disc; 600-sample addition body.
Detailed Description
The technical solutions of the present invention will be clearly and completely described in connection with the embodiments, and it is apparent that the described embodiments are some embodiments of the present invention, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.
As shown in fig. 1 to 3, the sample adding unit provided in this embodiment includes: a sample addition tube 100, a needle outer sleeve 200, a sealing mechanism 300 and a sample addition needle 400; the injection tube 100 is provided with a liquid injection channel 101, the needle cannula 200 is sleeved outside the injection tube 100, the sealing mechanism 300 is positioned at one end of the injection tube 100 close to the needle cannula 200, the sealing mechanism 300 is connected with the injection tube 100, and the sealing mechanism 300 is provided with a sealing channel 301 communicated with the liquid injection channel 101; the end of the needle outer sleeve 200, which is far away from the sample adding tube 100, is connected with the sample adding needle 400, the sample adding needle 400 extends into the needle outer sleeve 200, the liquid injection channel 101 is used for conveying liquid to the direction of the sample adding needle 400, the needle outer sleeve 200 is used for driving the sample adding needle 400 to move relative to the sample adding tube 100, so that the sample adding needle 400 is connected with or separated from the sealing channel 301, the liquid conveyed by the liquid injection channel 101 enters into the inner channel of the sample adding needle 400 or flows along the outer side wall of the sample adding needle 400, and a flow guide hole 201 for flowing the liquid along the outer side wall of the sample adding needle 400 is arranged between the sample adding needle 400 and the needle outer sleeve 200.
Optionally, the sample loading unit provided in this embodiment further includes a sample loading body 600, where the sample loading tube 100 and the needle outer sleeve 200 are located on the sample loading body 600, where the sample loading needle 400 can extend out of the sample loading body 600 through the sample loading body 600, where the sample loading body 600 is used as a basic structure of the sample loading unit, where the sample loading body 600 is mounted on a fixing frame of the cell extraction apparatus, and where the sample loading body 600 can provide a mounting space for the sample loading tube 100 and the needle outer sleeve 200, optionally, the sample loading body 600 may adopt a stainless steel housing, and where an opening is provided at one end of the sample loading body 600 close to the sample loading needle 400, so that a person can conveniently adjust the needle outer sleeve 200.
In the preferred embodiment of the present invention, the needle cannula 200 is threaded with the sample addition tube 100 and the end of the needle cannula 200 and the end of the sealing plug cover 302 are sealed; by using the threaded connection of the needle cannula 200 and the sample addition tube 100, the sample addition needle 400 can be driven to reciprocate in the vertical direction with respect to the sample addition tube 100 when the needle cannula 200 rotates with respect to the sample addition tube 100.
It should be noted that, in the sample adding unit provided in this embodiment, the dilution liquid can be injected into the experimental cup, after the injection of the dilution liquid is completed by the sample adding needle 400, the sample adding tube 100 and the needle outer sleeve 200 need to be cleaned at this time, wherein, when the sample adding needle 400 moves along with the needle outer sleeve 200, the sample adding needle 400 is connected to or separated from the sealing channel 301, and when the sample adding needle 400 is connected to the sealing channel 301, the sample adding needle 400 and the sealing mechanism 300 are in a sealed state, that is, the dilution liquid flows through the inside of the sample adding needle 400, the sample adding needle 400 extends into the experimental cup, and further, the possibility of contamination exists on the outside of the sample adding needle 400, so that, in order to clean the outer side wall of the sample adding needle 400, the needle outer sleeve 200 is connected to the sample adding needle 400, and the needle outer sleeve 200 can move relative to the sample adding needle 100, and when the sample adding needle 400 moves along with the needle outer sleeve 200, the sample adding needle 400 is connected to or separated from the sealing channel 301, and when the sample adding needle 400 is connected to the sealing channel 301, the sample adding needle 400 and the sealing mechanism 300 is in a sealed state, that the liquid conveyed by the sample adding needle 400 can only be conveyed to the inside the sealing channel, and the sample adding needle 400 can be cleaned by the cleaning liquid; when the sample adding needle 400 is separated from the sealing channel 301, the sample adding needle 400 and the sealing mechanism 300 are in a separated state, that is, the liquid conveyed by the sample adding tube 100 can flow along the outer wall of the sample adding needle 400 from the end part of the sample adding needle 400, and the inside and outside cleaning of the sample adding needle 400 is realized by arranging the flow guide hole 201 for flowing the liquid along the outer side wall of the sample adding needle 400 between the sample adding needle 400 and the needle outer sleeve 200, that is, the cleaning liquid can always flow along the outer side wall of the sample adding needle 400.
As shown in fig. 3, in the preferred embodiment of the present invention, a plurality of flow guide holes 201 are provided, and a plurality of flow guide holes 201 are provided at intervals along the circumferential direction of the loading needle 400, and the flow guide holes 201 are used to flow the liquid in the outer needle sheath 200 along the outer sidewall of the loading needle 400.
Optionally, the outer needle sleeve 200 is connected with the sample adding needle 400 through a partition board, and a guide hole 201 is arranged between any two adjacent partition boards, so that cleaning liquid in the outer needle sleeve 200 can flow along the circumferential side wall of the sample adding needle 400 by utilizing a plurality of guide holes 201, and the whole outer wall of the sample adding needle 400 is ensured to be cleaned.
The sample adding unit provided in this embodiment includes: a sample addition tube 100, a needle outer sleeve 200, a sealing mechanism 300 and a sample addition needle 400; the injection tube 100 is provided with a liquid injection channel 101, the needle cannula 200 is sleeved outside the injection tube 100, the sealing mechanism 300 is positioned at one end of the injection tube 100 close to the needle cannula 200, the sealing mechanism 300 is connected with the injection tube 100, and the sealing mechanism 300 is provided with a sealing channel 301 communicated with the liquid injection channel 101; one end of the needle outer sleeve 200, which is far away from the sampling tube 100, is connected with the sampling needle 400, the sampling needle 400 extends into the needle outer sleeve 200, the liquid injection channel 101 is used for conveying liquid to the direction of the sampling needle 400, the needle outer sleeve 200 is used for driving the sampling needle 400 to move relative to the sampling tube 100 so as to connect or separate the sampling needle 400 with the sealing channel 301, the liquid conveyed by the liquid injection channel 101 enters into the internal channel of the sampling needle 400 or flows along the outer side wall of the sampling needle 400, and a flow guide hole 201 for flowing the liquid along the outer side wall of the sampling needle 400 is arranged between the sampling needle 400 and the needle outer sleeve 200; namely, when the internal channel of the sample adding needle 400 needs to be cleaned, the needle outer sleeve 200 is utilized to drive the sample adding needle 400 to be in sealing connection with the sealing channel 301, so that liquid in the sample adding tube 100 can only enter the internal channel of the sample adding needle 400, when the external side wall of the sample adding needle 400 needs to be cleaned, the needle outer sleeve 200 is utilized to drive the sample adding needle 400 to be separated from the sealing channel 301 in a sealing manner, so that the liquid in the sample adding tube 100 can enter the needle outer sleeve 200 along the sealing channel 301, and the cleaning liquid can flow along the external side wall of the sample adding needle 400, the liquid can flow along the external side wall of the whole sample adding needle 400 by utilizing the guide hole 201, the effect of cleaning the inside and the outside of the sample adding needle 400 is realized, the technical problem that the sample adding needle 400 in the prior art can only clean the external wall of the sample adding needle 400 and the needle tube internal channel, and the possibility that the external wall pollutant of the next sample adding needle 400 can not pollute the subsequent sample extracting process is solved.
Further to the above embodiments, in a preferred embodiment of the present invention, the sealing mechanism 300 includes a sealing plug cover 302; the sealing channel 301 is located at the center of the sealing stopper 302, the sealing stopper 302 is fixedly connected to the end of the sample addition tube 100 along the circumferential direction of the sample addition tube 100, and the sample addition needle 400 is used for sealing connection with the sealing stopper 302.
In this embodiment, the sealing plug cover 302 is a plug body structure with a sealing channel 301 in the center, wherein the sealing plug cover 302 can be fixedly connected with the sampling tube 100, when the needle outer sleeve 200 drives the sampling needle 400 to extend into the sealing channel 301, the sealing plug cover 302 is in sealing connection with the sampling needle 400 at this time, when the needle outer sleeve 200 drives the sampling needle 400 to be separated from the sealing plug cover 302, the sealing channel 301 can realize the circulation of liquid at this time, and then the cleaning liquid can enter the inside of the needle outer sleeve 200.
As shown in FIG. 1, in the preferred embodiment of the present invention, the sealing channel 301 is in the shape of an inverted cone, and the inner diameter of the sealing channel 301 is smaller than the diameter of the sample addition needle 400, and the sample addition needle 400 extends into the sample addition tube 100 along the inclined surface of the sealing channel 301.
In this embodiment, in order to ensure that the loading needle 400 is in sealing connection with the sealing plug cover 302, the inner diameter of the sealing channel 301 is smaller than the diameter of the loading needle 400, in order to enable the loading needle 400 to better enter the sealing channel 301, and in order to ensure the tightness of the sealing channel 301, the loading needle 400 extends into the loading tube 100 along the inclined plane of the sealing channel 301, that is, the minimum end of the inclined plane of the sealing channel 301 is located at one side of the loading tube 100, so that the sealing of the sealing plug cover 302 can be ensured.
In the preferred embodiment of the present invention, the sealing stopper cap 302 is provided with a stepped groove, the sealing stopper cap 302 is clamped to the end of the sample adding tube 100 through the stepped groove, and the sealing stopper cap 302 is adhered to the sample adding tube 100 through the stepped groove.
Optionally, the sealing plug cover 302 can adopt a rubber sealing ring, and the sealing plug cover 302 is clamped with the end part of the sampling tube 100, and meanwhile, the sealing plug cover 302 and the position where the sampling tube 100 abuts can be bonded through fixing glue, so that the mounting stability of the sealing plug cover 302 is ensured.
As shown in fig. 2, in the preferred embodiment of the present invention, the sealing mechanism 300 further includes a fixed disk 303; the fixed disk 303 is sleeved outside the sample adding needle 400 along the circumferential direction of the sample adding needle 400, the fixed disk 303 is connected with one end of the sample adding needle 400 extending into the needle outer sleeve 200, the diameter of the fixed disk 303 is smaller than the inner diameter of the needle outer sleeve 200, the diameter of the fixed disk 303 is larger than the inner diameter of the sealing channel 301, and the fixed disk 303 is used for being abutted with the sealing plug cover 302 so that the sample adding needle 400 is in sealing connection with the sealing channel 301.
In this embodiment, the sealing plug cover 302 is fixedly sleeved on the outer wall of the end portion of the sample adding tube 100, the sealing plug cover 302 can adopt an annular sealing structure, an annular fixing disc 303 is fixed on the outer wall of the liquid inlet end of the sample adding needle 400, when the injection needle is tightly combined with the sealing plug cover 302, the liquid outlet hole on the sealing plug cover 302 is plugged by the fixing disc 303, liquid only flows out from the inner pipeline of the sample adding needle 400, and when the injection needle is separated from the sealing plug cover 302, the liquid in the liquid injecting channel 101 can flow out along the liquid outlet hole of the sealing plug cover 302 and the inner pipeline of the sample adding needle 400 together.
In a preferred embodiment of the present invention, a baffle 500 is also included; the flow guide disc 500 is located inside the needle outer sleeve 200, the flow guide disc 500 is connected with the inner wall of the needle outer sleeve 200, the flow guide disc 500 is located on one side of the fixed disc 303 away from the sealing plug cover 302, a flow guide hole 201 used for the sample injection needle 400 to penetrate through is formed in the center of the flow guide disc 500, the inner diameter of the flow guide hole 201 is larger than the outer diameter of the sample injection needle 400, and the flow guide disc 500 is used for guiding liquid flowing out of a gap between the sealing channel 301 and the fixed disc 303 so that the liquid flows along the surface of the outer side wall of the sample injection needle 400.
In the preferred embodiment of the present invention, the baffle 500 is inclined from one end to the other end near the fixed disk 303, and the liquid flowing out of the gap between the sealing passage 301 and the fixed disk 303 flows along the inclined surface of the baffle 500.
In this embodiment, when the cleaning solution in the sample adding tube 100 enters the needle outer sleeve 200 along with the sealing channel 301 of the sealing plug cover 302, the cleaning solution flows along with the fixing disc 303 to two sides of the sample adding needle 400, by being provided with the guide disc 500 which is obliquely arranged, the cleaning solution entering the needle outer sleeve 200 along with the fixing disc 303 contacts with the guide disc 500, and the cleaning solution is gathered again at the outer side wall of the sample adding needle 400 under the action of the guide disc 500, so that the cleaning solution is ensured to clean the outer side wall of the sample adding needle 400, and the design is more perfect.
The cell extraction instrument provided by the embodiment comprises the sample adding unit; since the technical effects of the cell extractor provided in this embodiment are the same as those of the sample adding unit provided in the above embodiment, the description thereof will not be repeated here.
Finally, it should be noted that: the above embodiments are only for illustrating the technical solution of the present invention, and not for limiting the same; although the invention has been described in detail with reference to the foregoing embodiments, it will be understood by those of ordinary skill in the art that: the technical scheme described in the foregoing embodiments can be modified or some or all of the technical features thereof can be replaced by equivalents; such modifications and substitutions do not depart from the spirit of the invention.
Claims (9)
1. A sample application unit comprising: the device comprises a sample adding tube, a needle outer sleeve, a sealing mechanism and a sample adding needle;
the needle-filling tube is provided with a liquid filling channel, the needle-coating tube is sleeved outside the liquid filling tube, the sealing mechanism is positioned at one end of the liquid filling tube, which is close to the needle-coating tube, and is connected with the liquid filling tube, and the sealing mechanism is provided with a sealing channel communicated with the liquid filling channel;
the outer needle sleeve is connected with the sample adding needle at one end far away from the sample adding tube, the sample adding needle stretches into the outer needle sleeve, the liquid injection channel is used for conveying liquid to the direction of the sample adding needle, the outer needle sleeve is used for driving the sample adding needle to move relative to the sample adding tube so as to enable the sample adding needle to be connected with or separated from the sealing channel, the liquid conveyed by the liquid injection channel enters into the inner channel of the sample adding needle or flows along the outer side wall of the sample adding needle, and a flow guide hole for enabling the liquid to flow along the outer side wall of the sample adding needle is arranged between the sample adding needle and the outer needle sleeve;
the sealing mechanism comprises a fixed disc; the fixed disk is sleeved outside the sample adding needle along the circumferential direction of the sample adding needle, and the fixed disk is connected with one end of the sample adding needle extending into the needle outer sleeve;
the air guide device also comprises a guide disc; the guide disc is positioned inside the needle outer sleeve, the guide disc is connected with the inner wall of the needle outer sleeve, a guide hole used for the sample adding needle to penetrate through is formed in the center of the guide disc, the inner diameter of the guide hole is larger than the outer diameter of the sample adding needle, and the guide disc is used for guiding liquid flowing out of a gap between the sealing channel and the fixing disc so that the liquid flows along the surface of the outer side wall of the sample adding needle.
2. The sample addition unit of claim 1, wherein the sealing mechanism further comprises a sealing plug cap;
the sealing channel is positioned in the center of the sealing plug cover, the sealing plug cover is fixedly connected with the end part of the sample adding pipe along the circumferential direction of the sample adding pipe, and the sample adding needle is used for being in sealing connection with the sealing plug cover;
the diameter of the fixed disk is smaller than the inner diameter of the needle outer sleeve, the diameter of the fixed disk is larger than the inner diameter of the sealing channel, and the fixed disk is used for being abutted to the sealing plug cover so that the sample adding needle is in sealing connection with the sealing channel.
3. The loading unit of claim 2 wherein the sealing channel is of inverted cone shape and the inner diameter of the sealing channel is smaller than the diameter of the loading needle, the loading needle extending into the loading tube along the inclined surface of the sealing channel.
4. A sample addition unit according to claim 3, wherein the sealing plug cover is provided with a stepped groove, the sealing plug cover is clamped at the end part of the sample addition pipe through the stepped groove, and the sealing plug cover is adhered with the sample addition pipe through the stepped groove.
5. The sample addition unit of claim 2, wherein the baffle is located on a side of the fixed disk remote from the sealing plug cover.
6. The sample addition unit according to claim 5, wherein the baffle plate is inclined from one end to the other end near the fixed plate, and the liquid flowing out of the gap between the sealing channel and the fixed plate flows along the inclined surface of the baffle plate.
7. The sample addition unit according to any one of claims 2 to 6, wherein the outer needle cannula is screwed to the sample addition tube and the outer needle cannula is sealed against the end of the sealing plug cap.
8. The sample addition unit according to claim 7, wherein a plurality of the flow guide holes are provided, the plurality of the flow guide holes being provided at intervals along the circumferential direction of the sample addition needle, the flow guide holes being for flowing the liquid in the needle outer sleeve along the outer side wall of the sample addition needle.
9. A cell extraction apparatus comprising a loading unit according to any one of claims 1 to 8.
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| Application Number | Priority Date | Filing Date | Title |
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| CN202110611292.2A CN113249199B (en) | 2021-06-01 | 2021-06-01 | Sample adding unit and cell extraction instrument |
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|---|---|---|---|
| CN202110611292.2A CN113249199B (en) | 2021-06-01 | 2021-06-01 | Sample adding unit and cell extraction instrument |
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| CN113249199A CN113249199A (en) | 2021-08-13 |
| CN113249199B true CN113249199B (en) | 2024-01-19 |
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