CN113249199A - Sample adding unit and cell extraction instrument - Google Patents
Sample adding unit and cell extraction instrument Download PDFInfo
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- CN113249199A CN113249199A CN202110611292.2A CN202110611292A CN113249199A CN 113249199 A CN113249199 A CN 113249199A CN 202110611292 A CN202110611292 A CN 202110611292A CN 113249199 A CN113249199 A CN 113249199A
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- 238000000605 extraction Methods 0.000 title claims abstract description 15
- 238000007789 sealing Methods 0.000 claims abstract description 104
- 239000007788 liquid Substances 0.000 claims abstract description 77
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 8
- 238000002347 injection Methods 0.000 claims description 44
- 239000007924 injection Substances 0.000 claims description 44
- 238000005406 washing Methods 0.000 abstract description 10
- 238000000926 separation method Methods 0.000 abstract description 6
- 230000000694 effects Effects 0.000 abstract description 5
- 239000003344 environmental pollutant Substances 0.000 abstract description 4
- 231100000719 pollutant Toxicity 0.000 abstract description 4
- 238000005070 sampling Methods 0.000 description 36
- 238000004140 cleaning Methods 0.000 description 12
- 210000004027 cell Anatomy 0.000 description 11
- 238000000034 method Methods 0.000 description 7
- 239000000243 solution Substances 0.000 description 6
- 239000003085 diluting agent Substances 0.000 description 5
- 238000005516 engineering process Methods 0.000 description 4
- 238000011109 contamination Methods 0.000 description 3
- 238000009434 installation Methods 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 238000005192 partition Methods 0.000 description 2
- 208000005443 Circulating Neoplastic Cells Diseases 0.000 description 1
- 239000000853 adhesive Substances 0.000 description 1
- 230000001070 adhesive effect Effects 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 238000003320 cell separation method Methods 0.000 description 1
- 239000000356 contaminant Substances 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 210000002615 epidermis Anatomy 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 239000012634 fragment Substances 0.000 description 1
- 210000000265 leukocyte Anatomy 0.000 description 1
- 238000001471 micro-filtration Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 210000000582 semen Anatomy 0.000 description 1
- -1 semen Substances 0.000 description 1
- 238000004544 sputter deposition Methods 0.000 description 1
- 229910001220 stainless steel Inorganic materials 0.000 description 1
- 239000010935 stainless steel Substances 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 210000001519 tissue Anatomy 0.000 description 1
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/38—Caps; Covers; Plugs; Pouring means
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B08—CLEANING
- B08B—CLEANING IN GENERAL; PREVENTION OF FOULING IN GENERAL
- B08B9/00—Cleaning hollow articles by methods or apparatus specially adapted thereto
- B08B9/02—Cleaning pipes or tubes or systems of pipes or tubes
- B08B9/023—Cleaning the external surface
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B08—CLEANING
- B08B—CLEANING IN GENERAL; PREVENTION OF FOULING IN GENERAL
- B08B9/00—Cleaning hollow articles by methods or apparatus specially adapted thereto
- B08B9/02—Cleaning pipes or tubes or systems of pipes or tubes
- B08B9/027—Cleaning the internal surfaces; Removal of blockages
- B08B9/032—Cleaning the internal surfaces; Removal of blockages by the mechanical action of a moving fluid, e.g. by flushing
- B08B9/0321—Cleaning the internal surfaces; Removal of blockages by the mechanical action of a moving fluid, e.g. by flushing using pressurised, pulsating or purging fluid
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
- C12M33/04—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by injection or suction, e.g. using pipettes, syringes, needles
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M37/00—Means for sterilizing, maintaining sterile conditions or avoiding chemical or biological contamination
- C12M37/04—Seals
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M39/00—Means for cleaning the apparatus or avoiding unwanted deposits of microorganisms
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- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Chemical & Material Sciences (AREA)
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- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Molecular Biology (AREA)
- Mechanical Engineering (AREA)
- Clinical Laboratory Science (AREA)
- Sampling And Sample Adjustment (AREA)
- Automatic Analysis And Handling Materials Therefor (AREA)
Abstract
The invention provides a sample adding unit and a cell extraction instrument, which relate to the technical field of cell extraction and comprise a sample adding tube, a needle outer sleeve, a sealing mechanism and a sample adding needle; when the internal channel of application of sample needle needs to be cleaned, utilize the needle outer tube to drive application of sample needle and sealed channel sealing connection, make the liquid in the application of sample pipe can only enter into the internal channel of application of sample needle, when the lateral wall of application of sample needle needs to be washd, utilize the needle outer tube to drive application of sample needle and sealed channel sealing separation, make the liquid of application of sample pipe can enter into the needle outer tube along sealed channel intraduct, and the washing liquid can flow along the lateral wall of application of sample needle, utilize the water conservancy diversion hole can make liquid flow along the outside lateral wall of whole application of sample needle, the inside and outside effect of all carrying out the washing to application of sample needle has been realized, the outer wall that exists can't wash application of sample needle among the prior art has been alleviated, the outer wall pollutant of application of sample needle can pollute the follow-up technical problem of drawing the sample.
Description
Technical Field
The invention relates to the technical field of cell extraction, in particular to a sample adding unit and a cell extraction instrument.
Background
In the prior art, the cell separation method of the mixed biological test material is mainly studied as follows: the method can directionally capture target cells with high precision, but has the problems of complicated sample pretreatment, expensive equipment consumables, complex operation and difficult popularization; in addition, the microfiltration technology, as a separation technology, plays an important role in the medical fields of leukocyte filtration and separation, separation and capture of circulating tumor cells, and the like, along with the development of materials science and preparation technology, especially the continuous progress of precision processing technology.
In the prior art, a sample (such as an adventitious sample such as epidermis fragments, semen, blood, a human tissue residual sample and the like) is placed in a sample cup and dissolved by injecting diluent in cell extraction equipment, a syringe needle is usually adopted to extend into a cup body space from an opening at the upper end of the sample cup, the syringe needle injects liquid by utilizing a needle hole at the end, and the filtered sample cup needs to be cleaned and filtered again so as to reduce sample loss and maximally collect a cell sample. However, in this process, since the injection has a certain fluid impact force, the vacuum at the end of the injection needle ejects the diluent along the vertical direction, and further generates a large amount of sputtering inevitably, and further causes contamination of the sample injection needle, it is necessary to clean the needle head to avoid contamination of the sample at the back in the next injection process.
However, in the cleaning process of the sample injection needle in the prior art, after the sample injection needle completes injection every time, the sample injection needle is moved to the cleaning position and then cleaning liquid is injected, but in this process, liquid only can clean the sample injection needle and an internal passage of the needle tube and cannot clean the outer wall of the sample injection needle, and then when the sample injection needle is used for injection, the possibility that subsequent injected liquid contacts with contaminants on the outer wall of the sample injection needle and further contaminates a subsequent sample extraction is still present.
Disclosure of Invention
The invention aims to provide a sample adding unit and a cell extraction instrument, and aims to solve the technical problems that in the prior art, in the cleaning process of a sample adding needle, only the sample adding needle and an internal channel of a needle tube can be cleaned, the outer wall of the sample adding needle cannot be cleaned, and the possibility that the outer wall of the sample adding needle for next injection can be polluted by pollutants on the outer wall of the sample adding needle for subsequent sample extraction is solved.
The invention provides a sample adding unit, which comprises: the sample adding device comprises a sample adding tube, a needle outer sleeve, a sealing mechanism and a sample adding needle;
the sample adding tube is provided with a liquid injection channel, the needle outer sleeve is sleeved outside the sample adding tube, the sealing mechanism is positioned at one end of the sample adding tube close to the needle outer sleeve, the sealing mechanism is connected with the sample adding tube, and the sealing mechanism is provided with a sealing channel communicated with the liquid injection channel;
the utility model discloses a liquid injection needle, including needle outer tube, injection needle, sealed channel, injection needle, needle outer tube, needle outer tube, needle, and needle.
In a preferred embodiment of the invention, the sealing mechanism comprises a sealing plug cap;
the sealed channel is located the center of sealed gag, sealed gag along the circumferencial direction of application of sample pipe with the tip fixed connection of application of sample pipe, the application of sample needle be used for with sealed gag sealing connection.
In a preferred embodiment of the present invention, the sealed channel is in an inverted cone shape, and an inner diameter of the sealed channel is smaller than a diameter of the sample injection needle, and the sample injection needle extends into the sample injection tube along an inclined plane of the sealed channel.
In a preferred embodiment of the present invention, the sealing plug cover is provided with a stepped groove, the sealing plug cover is clamped at an end of the sample adding pipe through the stepped groove, and the sealing plug cover is bonded with the sample adding pipe through the stepped groove.
In a preferred embodiment of the present invention, the sealing mechanism further comprises a fixed disk;
the fixed disk along the circumferencial direction cover of application of sample needle is located the outside of application of sample needle, the fixed disk with the application of sample needle stretches into the one end of needle outer tube is connected, the diameter of fixed disk is less than the internal diameter of needle outer tube, just the diameter of fixed disk is greater than the internal diameter of sealed passageway, the fixed disk be used for with sealed stopper cover butt, so that application of sample needle with sealed passageway is sealing connection.
In the preferred embodiment of the invention, the device also comprises a flow guide disc;
the flow guide disc is located inside the needle outer sleeve, the flow guide disc with the inner wall connection of needle outer sleeve, the flow guide disc is located the fixed disk is kept away from one side of sealed gag, the center of flow guide disc is provided with and is used for the water conservancy diversion hole that the application of sample needle runs through, the internal diameter in water conservancy diversion hole is greater than the external diameter of application of sample needle, the flow guide disc is used for right sealed passageway with the liquid of gap outflow between the fixed disk carries out the water conservancy diversion, so that liquid along the lateral wall surface flow of application of sample needle.
In a preferred embodiment of the present invention, the diversion disc is disposed in an inclined manner from one end close to the fixed disc to the other end, and the liquid flowing out from the gap between the sealing channel and the fixed disc flows along the inclined surface of the diversion disc.
In a preferred embodiment of the present invention, the needle outer sleeve is connected to the sample adding tube by a screw thread, and the needle outer sleeve is attached to and sealed with the end of the sealing plug.
In a preferred embodiment of the present invention, the flow guiding holes are provided in plurality, the flow guiding holes are arranged at intervals along a circumferential direction of the sample adding needle, and the flow guiding holes are used for allowing the liquid in the needle outer casing to flow along an outer side wall of the sample adding needle.
The cell extraction instrument provided by the invention comprises the sample adding unit.
The invention provides a sample adding unit, which comprises: the sample adding device comprises a sample adding tube, a needle outer sleeve, a sealing mechanism and a sample adding needle; the sampling tube is provided with a liquid injection channel, the needle outer sleeve is sleeved outside the sampling tube, the sealing mechanism is positioned at one end of the sampling tube close to the needle outer sleeve and connected with the sampling tube, and the sealing mechanism is provided with a sealing channel communicated with the liquid injection channel; one end, far away from the sample adding pipe, of the needle outer sleeve pipe is connected with the sample adding needle, the sample adding needle extends into the needle outer sleeve pipe, the liquid injection channel is used for conveying liquid to the direction of the sample adding needle, the needle outer sleeve pipe is used for driving the sample adding needle to move relative to the sample adding pipe so as to enable the sample adding needle to be connected with or separated from the sealing channel, the liquid conveyed by the liquid injection channel enters the internal channel of the sample adding needle or flows along the outer side wall of the sample adding needle, and a flow guide hole used for enabling the liquid to flow along the outer side wall of the sample adding needle is arranged between the sample adding needle and the needle outer sleeve pipe; namely, when the internal channel of the sample adding needle needs to be cleaned, the needle outer sleeve pipe drives the sample adding needle to be hermetically connected with the sealed channel, so that the liquid in the sample adding tube can only enter the internal channel of the sample adding needle, when the outer side wall of the sample adding needle needs to be cleaned, the needle outer sleeve tube is utilized to drive the sample adding needle to be separated from the sealing channel in a sealing way, so that the liquid in the sample adding tube can enter the needle outer sleeve tube along the sealing channel, the cleaning liquid can flow along the outer side wall of the sample adding needle, and the liquid can flow along the outer side wall of the whole sample adding needle by utilizing the flow guide holes, thereby realizing the effect of cleaning the inside and the outside of the sample adding needle, relieving the problem that the sample adding needle is cleaned in the prior art, can only wash application of sample needle and needle tubing internal passage, can't wash the outer wall of application of sample needle, the outer wall pollutant that exists next time and annotate liquid application of sample needle can pollute the follow-up technical problem who draws the sample possibility.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, and it is obvious that the drawings in the following description are some embodiments of the present invention, and other drawings can be obtained by those skilled in the art without creative efforts.
Fig. 1 is a schematic view of an overall structure of a sample adding unit according to an embodiment of the present invention;
fig. 2 is a schematic view of an overall structure of a sealing mechanism of a sample adding unit provided in an embodiment of the present invention, the sealing mechanism having a fixed disk;
fig. 3 is a schematic structural view of a connection position between a needle outer sleeve and a sample application needle of a sample application unit according to an embodiment of the present invention.
Icon: 100-a sample adding tube; 101-a liquid injection channel; 200-needle outer sleeve; 201-diversion holes; 300-a sealing mechanism; 301-sealing the channel; 302-sealing the stopper cap; 303-fixing the disc; 400-a sample adding needle; 500-a flow guiding disc; 600-sample application body.
Detailed Description
The technical solutions of the present invention will be described clearly and completely with reference to the following embodiments, and it should be understood that the described embodiments are some, but not all, embodiments of the present invention. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
As shown in fig. 1 to fig. 3, the sample adding unit provided in this embodiment includes: a sample adding tube 100, a needle outer sleeve 200, a sealing mechanism 300 and a sample adding needle 400; the sampling tube 100 is provided with a liquid injection channel 101, the needle outer sleeve 200 is sleeved outside the sampling tube 100, the sealing mechanism 300 is positioned at one end of the sampling tube 100 close to the needle outer sleeve 200, the sealing mechanism 300 is connected with the sampling tube 100, and the sealing mechanism 300 is provided with a sealing channel 301 communicated with the liquid injection channel 101; one end of the needle outer sleeve 200, which is far away from the sample adding tube 100, is connected with the sample adding needle 400, the sample adding needle 400 extends into the needle outer sleeve 200, the liquid injection channel 101 is used for conveying liquid to the direction of the sample adding needle 400, the needle outer sleeve 200 is used for driving the sample adding needle 400 to move relative to the sample adding tube 100, so that the sample adding needle 400 is connected with or separated from the sealing channel 301, so that the liquid conveyed by the liquid injection channel 101 enters the internal channel of the sample adding needle 400 or flows along the outer side wall of the sample adding needle 400, and a flow guide hole 201 for enabling the liquid to flow along the outer side wall of the sample adding needle 400 is arranged between the sample adding needle 400 and the needle outer sleeve 200.
Optionally, the sample adding unit provided in this embodiment further includes a sample adding main body 600, the sample adding tube 100 and the needle outer sleeve 200 are both located on the sample adding main body 600, the sample adding needle 400 can extend out of the sample adding main body 600 through the sample adding main body 600, the sample adding main body 600 serves as a basic structure of the sample adding unit, the sample adding main body 600 is installed on a fixing frame of the cell extraction instrument, and the sample adding main body 600 can provide an installation space for the sample adding tube 100 and the needle outer sleeve 200, optionally, the sample adding main body 600 can adopt a stainless steel shell, and one end of the sample adding main body 600 close to the sample adding needle 400 is provided with an opening, so that a person can conveniently adjust the needle outer sleeve 200.
In the preferred embodiment of the present invention, the needle outer sleeve 200 is connected to the sample adding tube 100 by a screw thread, and the end of the needle outer sleeve 200 and the end of the sealing plug 302 are sealed; through the needle outer sleeve 200 and the sample adding tube 100 that utilize threaded connection, when the needle outer sleeve 200 rotates for the sample adding tube 100, can drive the sample adding needle 400 for the sample adding tube 100 in vertical direction reciprocating motion.
It should be noted that the sample adding unit provided in this embodiment can inject and add a diluent into the cuvette, and after the sample adding needle 400 completes injecting the diluent, it is necessary to clean the sample adding needle 400, the sample adding tube 100 and the needle outer sleeve 200, wherein the sample adding tube 100 and the needle outer sleeve 200 are located inside the sample adding main body 600, and only the liquid injection channel 101 of the sample adding tube 100 and the inside of the needle outer sleeve 200 need to be cleaned, however, the diluent flows inside the sample adding needle 400, the sample adding needle 400 extends into the cuvette, and further there is a possibility of contamination outside the sample adding needle 400, in order to clean the outer sidewall of the sample adding needle 400, the needle outer sleeve 200 is connected to the sample adding needle 400, and the needle outer sleeve 200 can move relative to the sample adding tube 100, when the sample adding needle 400 moves along with the needle outer sleeve 200, at this time, the sampling needle 400 is connected or separated from the sealed channel 301, and when the sampling needle 400 is connected to the sealed channel 301, the sampling needle 400 and the sealing mechanism 300 are in a sealed state, that is, the liquid delivered by the sampling tube 100 can only be delivered to the internal channel of the sampling needle 400, and at this time, the liquid can be injected by using the sampling needle 400, and the internal channel of the sampling needle 400 can also be cleaned by using a cleaning liquid; when application of sample needle 400 and sealed channel 301 separate, application of sample needle 400 is in the separation state with sealing mechanism 300, and the liquid that application of sample pipe 100 carried promptly can flow along the outer wall of application of sample needle 400 from the tip of application of sample needle 400, is provided with the water conservancy diversion hole 201 that is used for flowing liquid along the lateral wall of application of sample needle 400 through being used for between application of sample needle 400 and needle outer tube 200, and the washing liquid can flow along the lateral wall of application of sample needle 400 always promptly, has realized washing the inside and outside of application of sample needle 400.
As shown in fig. 3, in the preferred embodiment of the present invention, a plurality of diversion holes 201 are provided, the plurality of diversion holes 201 are arranged at intervals along the circumferential direction of the sample injection needle 400, and the diversion holes 201 are used for allowing the liquid in the needle outer sleeve 200 to flow along the outer side wall of the sample injection needle 400.
Optionally, the needle outer sleeve 200 is connected to the sample adding needle 400 through a partition, a flow guide hole 201 is formed between any two adjacent partitions, and the cleaning liquid in the needle outer sleeve 200 can flow along the circumferential side wall of the sample adding needle 400 by using the flow guide holes 201, so that the cleaning of the entire outer wall of the sample adding needle 400 is ensured.
The sample adding unit provided by the embodiment comprises: a sample adding tube 100, a needle outer sleeve 200, a sealing mechanism 300 and a sample adding needle 400; the sampling tube 100 is provided with a liquid injection channel 101, the needle outer sleeve 200 is sleeved outside the sampling tube 100, the sealing mechanism 300 is positioned at one end of the sampling tube 100 close to the needle outer sleeve 200, the sealing mechanism 300 is connected with the sampling tube 100, and the sealing mechanism 300 is provided with a sealing channel 301 communicated with the liquid injection channel 101; one end of the needle outer sleeve 200, which is far away from the sample adding tube 100, is connected with the sample adding needle 400, the sample adding needle 400 extends into the needle outer sleeve 200, the liquid injection channel 101 is used for conveying liquid towards the direction of the sample adding needle 400, the needle outer sleeve 200 is used for driving the sample adding needle 400 to move relative to the sample adding tube 100, so that the sample adding needle 400 is connected with or separated from the sealing channel 301, the liquid conveyed by the liquid injection channel 101 enters the internal channel of the sample adding needle 400 or flows along the outer side wall of the sample adding needle 400, and a flow guide hole 201 for enabling the liquid to flow along the outer side wall of the sample adding needle 400 is arranged between the sample adding needle 400 and the needle outer sleeve 200; that is, when the internal channel of the sampling needle 400 needs to be cleaned, the needle outer sleeve 200 is utilized to drive the sampling needle 400 to be hermetically connected with the sealed channel 301, so that the liquid in the sampling tube 100 can only enter the internal channel of the sampling needle 400, when the outer side wall of the sampling needle 400 needs to be cleaned, the needle outer sleeve 200 is utilized to drive the sampling needle 400 to be hermetically separated from the sealed channel 301, so that the liquid in the sampling tube 100 can enter the needle outer sleeve 200 along the sealed channel 301, and the cleaning liquid can flow along the outer side wall of the sampling needle 400, the liquid can flow along the whole outer side wall of the sampling needle 400 by utilizing the flow guide hole 201, the effect of cleaning the inside and the outside of the sampling needle 400 is realized, the problem that the sampling needle 400 in the prior art can only clean the sampling needle 400 and the internal channel of the needle tube in the cleaning process is alleviated, the outer wall of the sampling needle 400 cannot be cleaned, and the technical problem that the outer wall pollutant of the next injection sampling needle 400 can pollute the possibility of subsequently extracting a sample exists.
On the basis of the above embodiments, further, in the preferred embodiment of the present invention, the sealing mechanism 300 includes a sealing plug cover 302; the sealing channel 301 is located at the center of the sealing plug 302, the sealing plug 302 is fixedly connected with the end of the sample adding tube 100 along the circumferential direction of the sample adding tube 100, and the sample adding needle 400 is used for being connected with the sealing plug 302 in a sealing manner.
In this embodiment, sealed gag 302 has the cock body structure of sealed passageway 301 for the center, wherein sealed gag 302 can with application of sample pipe 100 fixed connection, when needle outer tube 200 drives application of sample needle 400 and stretches into to sealed passageway 301, sealed gag 302 and application of sample needle 400 sealing connection this moment, when needle outer tube 200 drives application of sample needle 400 and sealed gag 302 separation, sealed passageway 301 can realize the circulation of liquid this moment, and then can make the washing liquid enter into inside the needle outer tube 200.
As shown in fig. 1, in the preferred embodiment of the present invention, the sealing channel 301 is an inverted cone, and the inner diameter of the sealing channel 301 is smaller than the diameter of the sampling needle 400, and the sampling needle 400 extends into the sampling tube 100 along the inclined surface of the sealing channel 301.
In this embodiment, in order to ensure that the loading needle 400 is connected with the sealing plug cover 302 in a sealing manner, the inner diameter of the sealing channel 301 is smaller than the diameter of the loading needle 400, in order to enable the loading needle 400 to better enter the sealing channel 301, and in order to ensure the sealing property of the sealing channel 301, the loading needle 400 extends into the loading tube 100 along the inclined plane of the sealing channel 301, that is, the minimum end of the inclined plane of the sealing channel 301 is located on one side of the loading tube 100, so that the sealing of the sealing plug cover 302 can be ensured.
In the preferred embodiment of the present invention, the sealing plug 302 is provided with a stepped groove, the sealing plug 302 is clamped at the end of the sample adding pipe 100 through the stepped groove, and the sealing plug 302 is bonded with the sample adding pipe 100 through the stepped groove.
Optionally, the sealing plug cover 302 may adopt a rubber sealing ring, and is clamped with the end of the sample adding pipe 100 through the sealing plug cover 302, and meanwhile, the positions of the sealing plug cover 302 abutted to the sample adding pipe 100 may be bonded through a fixing adhesive, so that the stability of the installation of the sealing plug cover 302 is ensured.
As shown in fig. 2, in the preferred embodiment of the present invention, the sealing mechanism 300 further includes a fixed disk 303; the fixing disc 303 is sleeved outside the sample adding needle 400 along the circumferential direction of the sample adding needle 400, the fixing disc 303 is connected with one end, extending into the needle outer sleeve 200, of the sample adding needle 400, the diameter of the fixing disc 303 is smaller than the inner diameter of the needle outer sleeve 200, the diameter of the fixing disc 303 is larger than the inner diameter of the sealing channel 301, and the fixing disc 303 is used for being abutted to the sealing plug cover 302, so that the sample adding needle 400 is in sealing connection with the sealing channel 301.
In this embodiment, the fixed cover of sealing plug cover 302 is established on the tip outer wall of application of sample pipe 100, sealing plug cover 302 can adopt annular seal structure, the outer wall of the feed liquor end of application of sample needle 400 is fixed with annular fixed disk 303, when syringe needle and sealing plug cover 302 closely combine, play liquid hole on sealing plug cover 302 is blocked by fixed disk 303, liquid only flows from application of sample needle 400 internal pipeline, when syringe needle and sealing plug cover 302 separate, liquid can flow out with application of sample needle 400 internal pipeline jointly along the play liquid hole of sealing plug cover 302 in the notes liquid channel 101.
In the preferred embodiment of the present invention, the present invention further comprises a diversion plate 500; the flow guide disc 500 is located inside the needle outer sleeve 200, the flow guide disc 500 is connected with the inner wall of the needle outer sleeve 200, the flow guide disc 500 is located on one side of the fixing disc 303 far away from the sealing plug cover 302, the center of the flow guide disc 500 is provided with a flow guide hole 201 for the sample adding needle 400 to run through, the inner diameter of the flow guide hole 201 is larger than the outer diameter of the sample adding needle 400, the flow guide disc 500 is used for guiding liquid flowing out from a gap between the sealing channel 301 and the fixing disc 303, so that the liquid flows along the surface of the outer side wall of the sample adding needle 400.
In the preferred embodiment of the present invention, the diversion disc 500 is disposed to be inclined from one end to the other end near the fixed disc 303, and the liquid flowing out from the gap between the sealing passage 301 and the fixed disc 303 flows along the inclined surface of the diversion disc 500.
In this embodiment, when the washing liquid in the application of sample pipe 100 enters into needle outer tube 200 along with sealed passageway 301 of sealed gag 302 inside, the washing liquid can flow to the both sides of application of sample needle 400 along with fixed disk 303 this moment, through being provided with the guiding disk 500 that is the slope setting, the washing liquid that enters into needle outer tube 200 along with fixed disk 303 can contact with guiding disk 500, the washing liquid can gather at the lateral wall position of application of sample needle 400 again under guiding disk 500's effect, the washing liquid has been guaranteed to wash the lateral wall of application of sample needle 400, make the design more perfect.
The cell extraction instrument provided by the embodiment comprises the sample adding unit; since the technical effect of the cell extraction instrument provided in this embodiment is the same as that of the sample adding unit provided in the above embodiment, details thereof are not repeated here.
Finally, it should be noted that: the above embodiments are only used to illustrate the technical solution of the present invention, and not to limit the same; while the invention has been described in detail and with reference to the foregoing embodiments, it will be understood by those skilled in the art that: the technical solutions described in the foregoing embodiments may still be modified, or some or all of the technical features may be equivalently replaced; and the modifications or the substitutions do not make the essence of the corresponding technical solutions depart from the scope of the technical solutions of the embodiments of the present invention.
Claims (10)
1. A sample addition unit, comprising: the sample adding device comprises a sample adding tube, a needle outer sleeve, a sealing mechanism and a sample adding needle;
the sample adding tube is provided with a liquid injection channel, the needle outer sleeve is sleeved outside the sample adding tube, the sealing mechanism is positioned at one end of the sample adding tube close to the needle outer sleeve, the sealing mechanism is connected with the sample adding tube, and the sealing mechanism is provided with a sealing channel communicated with the liquid injection channel;
the utility model discloses a liquid injection needle, including needle outer tube, injection needle, sealed channel, injection needle, needle outer tube, needle outer tube, needle, and needle.
2. The sample addition unit according to claim 1, wherein the sealing mechanism comprises a sealing plug cover;
the sealed channel is located the center of sealed gag, sealed gag along the circumferencial direction of application of sample pipe with the tip fixed connection of application of sample pipe, the application of sample needle be used for with sealed gag sealing connection.
3. The sample application unit according to claim 2, wherein the sealed channel is an inverted cone, and the inner diameter of the sealed channel is smaller than the diameter of the sample application needle, and the sample application needle extends into the sample application tube along the inclined surface of the sealed channel.
4. The sample adding unit according to claim 3, wherein the sealing plug cover is provided with a stepped groove, the sealing plug cover is clamped at the end part of the sample adding pipe through the stepped groove, and the sealing plug cover is bonded with the sample adding pipe through the stepped groove.
5. The sample addition unit according to claim 2, wherein the sealing mechanism further comprises a fixed disk;
the fixed disk along the circumferencial direction cover of application of sample needle is located the outside of application of sample needle, the fixed disk with the application of sample needle stretches into the one end of needle outer tube is connected, the diameter of fixed disk is less than the internal diameter of needle outer tube, just the diameter of fixed disk is greater than the internal diameter of sealed passageway, the fixed disk be used for with sealed stopper cover butt, so that application of sample needle with sealed passageway is sealing connection.
6. The sample adding unit according to claim 5, further comprising a flow guide disc;
the flow guide disc is located inside the needle outer sleeve, the flow guide disc with the inner wall connection of needle outer sleeve, the flow guide disc is located the fixed disk is kept away from one side of sealed gag, the center of flow guide disc is provided with and is used for the water conservancy diversion hole that the application of sample needle runs through, the internal diameter in water conservancy diversion hole is greater than the external diameter of application of sample needle, the flow guide disc is used for right sealed passageway with the liquid of gap outflow between the fixed disk carries out the water conservancy diversion, so that liquid along the lateral wall surface flow of application of sample needle.
7. The sample adding unit of claim 6, wherein the diversion disc is disposed along an inclined plane from one end near the fixed disc to the other end, and the liquid flowing out from the gap between the sealing channel and the fixed disc flows along the inclined plane of the diversion disc.
8. The sample adding unit according to any one of claims 2 to 7, wherein the needle outer sleeve is connected with the sample adding tube by a screw thread, and the needle outer sleeve is sealed with the end of the sealing plug cover.
9. The sample adding unit of claim 8, wherein the flow guiding holes are provided in plurality, and the flow guiding holes are arranged at intervals along the circumferential direction of the sample adding needle, and the flow guiding holes are used for flowing the liquid in the needle outer casing tube along the outer side wall of the sample adding needle.
10. A cell extraction instrument comprising the sample addition unit according to any one of claims 1 to 9.
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| CN202110611292.2A CN113249199B (en) | 2021-06-01 | 2021-06-01 | Sample adding unit and cell extraction instrument |
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| CN202110611292.2A CN113249199B (en) | 2021-06-01 | 2021-06-01 | Sample adding unit and cell extraction instrument |
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