CN113229421A - Aphidius civoris feed and feeding method thereof - Google Patents
Aphidius civoris feed and feeding method thereof Download PDFInfo
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/90—Feeding-stuffs specially adapted for particular animals for insects, e.g. bees or silkworms
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/20—Animal feeding-stuffs from material of animal origin
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/163—Sugars; Polysaccharides
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Abstract
The invention relates to an aphid eating gall midge feed and a feeding method thereof, wherein the feed is prepared from the following raw materials in parts by weight: 200-300 parts of aphid juice, 50-100 parts of wheat germ juice, 200-300 parts of barley seedling juice, 10-30 parts of cane sugar and 0.02-0.05 part of food preservative. The 5 raw materials are mutually matched and supplemented, so that the aphid eating gall midge feed which is high in egg laying amount, stable in egg laying quality, high in hatching rate and high in survival rate of 1-instar larvae is obtained. In the feeding method provided by the invention, the adult and the 1 st larva are fed with liquid feed, and the 2 nd and 3 rd larvae are preyed by aphids, the aphid eating gall midge eggs obtained by the method are very full, the hatching rate is high, the death rate of the 1 st larva is low, and the condition of starvation caused by poor foraging capability of the larvae in the conventional feeding method is avoided.
Description
Technical Field
The invention relates to an aphid eating gall midge feed and a feeding method thereof, belonging to the field of feeding aphid eating gall midge.
Background
This background of the invention section of information is intended only to add some insight to the general background of the invention, and is not necessarily to be construed as an acknowledgement or any form of suggestion that this information forms the prior art already known to a person skilled in the art.
The aphid eating cecidomyiia belongs to diptera cecidomycidae, is a specific predatory natural enemy of aphids, can prey on aphids on 61 different crops, is an effective natural enemy for controlling the aphids as pests, particularly has good control effect on greenhouse aphids, and is widely applied in the world.
The conventional method for feeding the aphidius gifuensis mainly comprises the following steps: (1) clean seedling cultivation; (2) feeding aphids; (3) inoculating the aphid eating cecidomyiia; (4) breeding aphid eating gall midge by using the raised aphids. For example, patent CN100405900A, CN107711723A, etc., although these methods can obtain a certain amount of aphid eating gall midge, these methods still have the problems of low egg laying amount, unstable egg laying quality, low hatching rate, low survival rate of 1-instar larvae, etc.
Disclosure of Invention
Aiming at the background technologies, the invention aims to provide a method and feed for feeding aphidicolvorax aphidicollis with higher egg laying amount, more stable egg laying quality, higher hatching rate and higher survival rate of 1-instar larvae, and provides a method and a process for feeding aphidicolvorax aphidicollis more stably, more standardizedly and more normatively.
Specifically, the invention adopts the following technical scheme:
in a first aspect of the invention, the invention provides an aphidius gifuensis feed, which is prepared from the following raw materials in parts by weight:
200-300 parts of aphid juice, 50-100 parts of wheat germ juice, 200-300 parts of barley seedling juice, 10-30 parts of cane sugar and 0.02-0.05 part of food preservative;
the aphid juice is prepared by the following method:
1) and (3) cultivating clean seedlings:
selecting barley seeds without pesticide residues, soaking the seeds for 8-12 hours, planting the barley, and cultivating clean seedlings;
2) breeding aphids:
breeding aphids by using clean seedlings for 48-72 h;
3) cultivating aphids on an intermittent shaking table:
culturing the aphids bred in the step 2) by adopting an intermittent shaking table, wherein the culturing time is 12-24 h, the shaking time is 3-6 s each time, the pause time is 60-120 s, and the amplitude is 10-20 mm;
4) collecting aphids and secreted substances:
collecting the aphids cultivated in the step 3) and substances secreted by the aphids, pulping the aphids, and filtering to obtain an aphid supernatant; and (3) fully dissolving the secreted substances by adopting aphid supernatant to obtain aphid juice.
In one or more embodiments of the invention, in step 2), the aphids are corn aphids and/or cereal aphids. According to the invention, various aphids are screened for testing, and the test proves that compared with other aphids, the corn aphids and/or the cereal aphids with the constriction duct can ensure higher egg laying amount, more stable egg laying quality, higher hatching rate and higher survival rate of 1-instar larvae.
The invention firstly adopts a standing cultivation mode to cultivate aphids, and aims to obtain the aphids with larger yield, and if shaking cultivation is carried out at the beginning, the aphids are very easy to be frightened, and the growth, development and yield of the aphids are influenced. The adopted parameters of the intermittent shaking table (the shaking time is 3-6 s each time, the pause time is 60-120 s each time, and the amplitude is 10-20 mm) meet the biological characteristics of aphids, so that the aphids can generate a large amount of molasses.
According to the invention, raw materials and the proportion of the aphid eating cecidomyiia feed are researched and searched, if aphid dry powder, wheat germ powder and barley seedling dry powder are adopted to replace aphid juice, wheat germ juice and barley seedling juice, 1-year-old larvae are small, powdery nutrient substances cannot be effectively absorbed and digested, and the death rate of the aphid eating cecidomyiia feed is increased through experimental verification; the aphid juice, the wheat germ juice and the barley seedling juice are adopted, so that the absorption and digestion of the breeding mosquitoes are facilitated, and the subsequent growth and development progress of the aphid eating gall midge is more consistent through experimental verification; if food preservatives are not used, microorganisms such as mold and the like are easily bred in the feed.
In one or more embodiments of the present invention, the barley seedling juice is prepared by the following method:
pulping and filtering the clean seedlings to obtain barley seedling juice.
In one or more embodiments of the invention, the wheat germ juice is obtained by beating and filtering wheat germs.
In one or more embodiments of the invention, the food preservative is potassium sorbate or benzoic acid.
In a second aspect of the present invention, there is provided a process for preparing a aphidicolesium feed, the process comprising the steps of:
firstly, adding cane sugar and a food preservative into barley seedling juice for full dissolution, and then uniformly mixing the barley seedling juice, the aphid juice and the wheat germ juice to obtain the aphid eating gall midge feed.
In a third aspect of the invention, there is provided the use of said aphidicolus feeding feed for feeding aphidicolus.
In a fourth aspect of the present invention, there is provided a method for feeding aphidicolophagus, which comprises feeding aphidicolus with said aphidicolus feed.
In one or more embodiments of the present invention, the method for feeding aphidicolina specifically comprises the following steps:
(1) pouring the aphid eating gall midge feed into a feeding disc, placing a wire mesh on the feeding disc, and then moving the feeding disc into an egg receiving chamber;
(2) inoculating the aphid eating cecidomyiia imago into an egg inoculating chamber, and mating and inoculating eggs;
(3) moving the egg laying feeding plate (with a wire mesh) into an insect room, moving the barley seedlings with aphids into the insect room when the larvae are 2 years old until the larvae grow to mature in the 6 th to 7 th days, and moving the barley seedlings out of the insect room;
(4) and (3) inclining the aged larvae into water, jumping the aged larvae into the water, collecting the aged larvae in the water by using a suction pipe, and transferring the collected aged larvae into a pupation bottle.
In one or more embodiments of the invention, the mating and egg receiving time in step (2) is 24h in total.
In one or more embodiments of the invention, in step (3), the habitat is removed until the 7 th day develops to an aged larva.
Compared with the related technology known by the inventor, one technical scheme of the invention has the following beneficial effects:
(1) the aphid eating gall midge feed provided by the invention is mainly developed aiming at adults and 1-year-old larvae in mating and egg receiving periods, wherein aphid juice is prepared by adopting a specific method, and aiming at the growth characteristics of aphids, a method of firstly standing and cultivating and then indirectly cultivating by using a shaking table is adopted, so that the aphids not only can be rapidly propagated, but also can secrete a large amount of molasses, and the aphid eating gall midge feed has great attraction to the adults and 1-year-old larvae in the mating and egg receiving periods. If the aphid is only cultured by a shaking table, the aphid is easy to be frightened and cannot be propagated in large quantity and grow normally, and if the aphid is only cultured by standing, the secreted molasses substances cannot be effectively obtained. The wheat germ juice contains abundant VE, nucleic acid, folic acid and other substances, provides necessary nutrition for the breeding of adults, has high egg quantity and egg quality, has rich and balanced nutrient contents in the barley seedling juice, and is particularly suitable for supplying adults and 1-instar larvae with necessary nutrient components. The cane sugar can supplement more energy for adults and 1-instar larvae, and the food preservative prevents liquid feed containing sugar from breeding microorganisms such as mold and the like. The feed adopts a nutrient solution form, so that the adult and 1-instar larva consume less energy when eating, and are easier to absorb and digest. The 5 raw materials are mutually matched and supplemented, so that the aphid eating gall midge feed which is high in egg laying amount, stable in egg laying quality, high in hatching rate and high in survival rate of 1-instar larvae is obtained.
(2) In the feeding method provided by the invention, the adult and the 1 st larva are fed with liquid feed, and the 2 nd and 3 rd larvae are preyed by aphids, the aphid eating gall midge eggs obtained by the method are very full, the hatching rate is high, the death rate of the 1 st larva is low, and the condition of starvation caused by poor foraging capability of the larvae in the conventional feeding method is avoided.
(3) In the feeding method provided by the invention, the larvae develop more regularly, and the pupation progress is more consistent.
Drawings
The accompanying drawings, which are incorporated in and constitute a part of this specification, illustrate an embodiment of the invention and, together with the description, serve to explain the invention and not to limit the invention.
FIG. 1 shows a feed liquid for aphidicoling mosquitoes in an embodiment of the present invention.
Fig. 2 is a schematic top view of a mesh placed in a rearing tray in an embodiment of the present invention.
Detailed Description
It is to be understood that the following detailed description is exemplary and is intended to provide further explanation of the invention as claimed. Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs.
It is noted that the terminology used herein is for the purpose of describing particular embodiments only and is not intended to be limiting of exemplary embodiments according to the invention. As used herein, the singular forms "a", "an" and "the" are intended to include the plural forms as well, and it should be understood that when the terms "comprises" and/or "comprising" are used in this specification, they specify the presence of the stated features, steps, operations, and/or combinations thereof, unless the context clearly indicates otherwise.
In order to make the technical solutions of the present invention more clearly understood by those skilled in the art, the technical solutions of the present invention will be described in detail below with reference to specific embodiments.
Example 1
An aphid eating gall midge feed is prepared from the following raw materials in parts by weight:
280 parts of aphid juice, 60 parts of wheat germ juice, 260 parts of barley seedling juice, 15 parts of cane sugar and 0.02 part of food preservative, namely benzoic acid.
The aphid eating gall midge feed is prepared by the following method:
(1) preparation of aphid juice:
selecting barley seeds without pesticide residues, soaking the barley seeds in water for 10 hours, planting barley in a greenhouse, culturing clean seedlings, and dividing the clean seedlings into two parts for later use;
after the barley seedlings emerge, the barley seedlings are moved into an aphid raising chamber, the cereal aphids are inoculated, the temperature in the aphid raising chamber is controlled to be about 25 ℃, the relative humidity is about 65%, the illumination is kept for about 14 hours every day, and the illumination intensity is 2500 lx; the breeding time is 3 d; after breeding for 3d, transferring the barley seedlings with the aphids into a shake flask for intermittent shake culture, wherein the culture time is 18h, each shake time is 3s, the pause time is 90s, and the amplitude is 20 mm; after culturing for 18h, collecting aphids subjected to intermittent culture, pulping the collected aphids, and removing solid substances by suction filtration to obtain aphid supernatant; scraping substances secreted by aphids on barley seedlings, wherein the main component is molasses, and stirring the secreted substances by adopting aphid supernatant to fully dissolve the substances to obtain aphid juice for later use;
(2) preparing wheat germ juice:
pulping wheat germ, and filtering to obtain wheat germ juice;
(3) preparing barley seedling juice:
pulping and filtering part of the clean seedlings cultured in the step (1) to obtain barley seedling juice for later use;
(4) preparing an aphid eating gall midge feed:
the set weight parts of sucrose and benzoic acid were added to the barley seedling juice and dissolved thoroughly, and then mixed well with the aphid juice and wheat germ juice to obtain a clear green level aphid eating gall midge feed liquid as shown in fig. 1, which has a low viscosity and does not affect or restrict the activities of adults and 1 st instar larvae.
Example 2
An aphid eating gall midge feed is prepared from the following raw materials in parts by weight:
260 parts of aphid juice, 75 parts of wheat germ juice, 260 parts of barley seedling juice, 20 parts of cane sugar and 0.02 part of food preservative potassium sorbate.
The aphid eating gall midge feed is prepared by the following method:
(1) preparation of aphid juice:
selecting barley seeds without pesticide residues, soaking the barley seeds in water for 12 hours, planting barley in a greenhouse, culturing clean seedlings, and dividing the clean seedlings into two parts for later use;
after the barley seedlings emerge, the barley seedlings are moved into an aphid raising chamber, the cereal aphids are inoculated, the temperature in the aphid raising chamber is controlled to be about 25 ℃, the relative humidity is about 65%, the illumination is kept for about 14 hours every day, and the illumination intensity is 3000 lx; the breeding time is 3 d; after breeding for 3d, transferring the barley seedlings with aphids into a shake flask for intermittent shake cultivation, wherein the cultivation time is 24h, each shake time is 5s, the pause time is 120s, and the amplitude is 20 mm; after culturing for 24h, collecting aphids subjected to intermittent culture, pulping the collected aphids, and removing solid substances by suction filtration to obtain aphid supernatant; scraping substances secreted by aphids on barley seedlings, wherein the main component is molasses, and stirring the secreted substances by adopting aphid supernatant to fully dissolve the substances to obtain aphid juice for later use;
(2) preparing wheat germ juice:
pulping wheat germ, and filtering to obtain wheat germ juice;
(3) preparing barley seedling juice:
pulping and filtering part of the clean seedlings cultured in the step (1) to obtain barley seedling juice for later use;
(4) preparing an aphid eating gall midge feed:
adding the set parts by weight of sucrose and benzoic acid into the barley seedling juice for sufficient dissolution, and then uniformly mixing the barley seedling juice, the aphid juice and the wheat germ juice to obtain the aphid eating gall midge feed.
Example 3
An aphid eating gall midge feed is prepared from the following raw materials in parts by weight:
265 parts of aphid juice, 65 parts of wheat germ juice, 260 parts of barley seedling juice, 25 parts of cane sugar and 0.02 part of food preservative-benzoic acid.
The aphid eating gall midge feed is prepared by the following method:
(1) preparation of aphid juice:
selecting barley seeds without pesticide residues, soaking the barley seeds in water for 8 hours, planting barley in a greenhouse, culturing clean seedlings, and dividing the clean seedlings into two parts for later use;
after the barley seedlings emerge, the barley seedlings are moved into an aphid raising chamber, the cereal aphids are inoculated, the temperature in the aphid raising chamber is controlled to be about 25 ℃, the relative humidity is about 65%, the illumination is kept for about 14 hours every day, and the illumination intensity is 2500 lx; the breeding time is 3 d; after breeding for 3d, transferring the barley seedlings with aphids into a shake flask for intermittent shake culture, wherein the culture time is 20h, each shake time is 5s, the pause time is 90s, and the amplitude is 20 mm; after 20h of cultivation, collecting aphids subjected to intermittent cultivation, pulping the collected aphids, and removing solid matters by suction filtration to obtain aphid supernatant; scraping substances secreted by aphids on barley seedlings, wherein the main component is molasses, and stirring the secreted substances by adopting aphid supernatant to fully dissolve the substances to obtain aphid juice for later use;
(2) preparing wheat germ juice:
pulping wheat germ, and filtering to obtain wheat germ juice;
(3) preparing barley seedling juice:
pulping and filtering part of the clean seedlings cultured in the step (1) to obtain barley seedling juice for later use;
(4) preparing an aphid eating gall midge feed:
adding the set parts by weight of sucrose and benzoic acid into the barley seedling juice for sufficient dissolution, and then uniformly mixing the barley seedling juice, the aphid juice and the wheat germ juice to obtain the aphid eating gall midge feed.
Test example 1
A method for feeding cecidomyiia aphidicola specifically comprises the following steps:
(1) the aphidius gifuensis feed from example 1 was poured into a feeding tray, on which a multi-layer sterile gauze was placed as shown in fig. 2, and was moved into an oviposition chamber;
(2) inoculating the aphid eating cecidomyiia imago to an egg inoculating chamber, and naturally inoculating eggs for 24 h;
(3) transferring the egg-laid rearing tray (with silk screen) into an insect chamber until the larva of 2 th age (after hatching, the larva of 1 st molt is the larva after the first molt, wherein the larva of 2 nd age is the larva after the first molt, and about 2 days after hatching), transferring the barley seedling with aphid into the insect chamber until the 7 th day is developed into the mature larva, and then transferring out of the insect chamber;
(4) inclining the aged larvae into water, allowing the aged larvae to jump into water, collecting the aged larvae in water with a suction pipe, transferring into a pupation bottle for conventional pupation, and arranging wet pupation matrix-vermiculite in the pupation bottle.
Comparative example 1
A method for feeding cecidomyiia aphidicola specifically comprises the following steps:
(1) clean seedling is cultivated
Selecting barley without pesticide residue, soaking seeds for 10h, planting the barley, culturing clean seedlings, and using after 7 d;
(2) breeding aphids
And (4) breeding the aphids of the sinonovacula avenae by using the barley seedlings for 3 d.
(3) Breeding of aphid eating gall midge
Breeding and cultivating aphids for feeding aphid eating cecidomyiia, and transferring to an aphid eating cecidomycidomycidae egg-receiving chamber for receiving mosquitoes when the aphid amount on the barley seedlings in an aphid feeding tray is about 1000; and (3) after 24h of mosquito inoculation, removing the barley seedlings, putting the barley seedlings into an insect breeding chamber, breeding for 7d, growing aphid eating gall midge eggs to mature larvae, inclining the barley seedlings into water, jumping the mature larvae into the water, collecting the mature larvae, and pupating the mature larvae in a wet breeding bottle.
Note: experimental example 1 and comparative example 1 the adult aphidius gifuensis to be inoculated into the egg inoculation chamber was collected from the experimental field in the academy of agricultural sciences of Shandong province and tested after indoor breeding for 2-3 generations, and the retention ratio of the two groups of experiments was the same.
1. Hatching data statistics
After the eggs were inoculated for 24 hours, the hatching conditions of 150 eggs in the test example 1 and the comparative example 1 were observed, and the number of unhatched eggs and the egg mortality were counted and repeated 3 times.
The statistical and computational results are shown in table 1.
Results: the average mortality rate in egg phase of test example 1 was 3.56%, and the average mortality rate in egg phase of comparative example 1 was 12.67%, which were significantly different. Through observing a plurality of repeated eggs, compared with comparative example 1, the aphid-eating gall mosquito egg of the experimental example 1 is more plump in shape, more in number of effective eggs (male and female mating obtained), and the hatching rate is greatly improved.
This shows that the aphid eating gall midge feed adopted in the step (1) of the experimental example is more beneficial to supplement adult energy and attract male and female mating, so that the egg hatchability is higher.
2. Statistics of 1 st, 2 nd and 3 rd larva death data
The method of test example 1 and comparative example 1 was used to breed eggs into 1 st, 2 nd and 3 rd instar larvae, and the number of dead insects and the calculated mortality rate were counted from egg to 1 st, egg to 2 nd and egg to 3 rd instar larvae, and were repeated 3 times.
The statistical and computational results are shown in table 2.
Results：
In test example 1, the overall average mortality rate from egg to 1 st larva period was 8%, including the unhatched egg mortality rate (table 1, 3.56%) and the 1 st larva mortality rate, then the mortality rate for 1 st larva was 4.44%; for the egg to 2 instar larva period, the overall average mortality rate is 11.89%, including egg to 1 instar larva mortality rate (8%) and 1 instar to 2 instar larva mortality rate, then 1 instar to 2 instar larva mortality rate is 3.89%; at the egg to 3 instar larva stage, the overall average mortality rate is 14.89%, including egg to 2 instar larva mortality rate (11.89%) and 3 instar larva mortality rate, then 3 instar larva mortality rate is 3%.
In comparative example 1, the overall average mortality rate from egg to 1 instar larva was 28.66%, including unhatched egg mortality (table 1, 12.67%) and 1 instar larva mortality, then the mortality rate for 1 instar larva was 15.99%; for the egg to 2 instar larva period, the overall average mortality rate is 33.56%, including egg to 1 instar larva mortality rate (28.66%) and 1 instar to 2 instar larva mortality rate, then 1 instar to 2 instar larva mortality rate is 4.9%; at the egg to 3 instar larva stage, the overall average mortality rate was 37.44%, including egg to 2 instar larva mortality rate (33.56%) and 3 instar larva mortality rate, then 3 instar larva mortality rate was 3.88%.
In the test of comparative example 1, the mortality rate was higher for larvae at 1 st, compared to test example 1, because larvae at 1 st were very small, had poor mobile foraging ability, and were unable to move rapidly to their side for killing and feeding when they were further away from aphids, resulting in the death of larvae at 1 st. In the test of the test example 1, the requirement on the mobility and the predation capacity of the larvae of 1 st age is low because the liquid nutritional feed is adopted, the larvae of 1 st age can absorb the liquid nutritional feed in time, sufficient and rich nutrition is provided for the smooth growth of the larvae of older age, and the death rate is obviously reduced compared with the comparative example 1. For the 2-to 3-instar aphidius gifuensis larvae, the foraging and moving capacities of the 2-to 3-instar larvae were high and the mortality rate was low in test example 1 and comparative example 1.
3. Statistics of pupation, eclosion and egg laying
Pupating is carried out in an incubator at 22 ℃ according to the test example 1 and the comparative example 1, and the pupation rate is counted after 2 d; removing un-pupated larva, retaining pupated larva in pupation bottle, and culturing in 22 deg.C incubator. After the adults begin to eclose, the bottle caps of the pupation bottles are removed, the adults which are successfully eclosion fly out of the pupation bottles into an egg receiving chamber, sufficient barley seedlings and aphids are placed in the egg receiving chamber, the barley seedlings are replaced every day, the egg amount is counted, and the process is repeated for 3 times.
The statistical and computational results are shown in table 3.
Results：
Pupation condition: because the larvae in the pupation bottle are not pupated in a unified way, some larvae are not pupated after 2d, the subsequent eclosion progress is inconsistent, and the earlier eclosion insects can gnaw and bite the un-eclosion pupation. Compared with the comparative example 1, the pupation rate of the experimental example 1 is lower, which indicates that the pupation progress is more consistent.
Eclosion conditions: the average feathering rate in test example 1 was 94.17%, and the mortality rate was 5.83%; the average feathering rate was 85.67% and the mortality rate was 14.33% in comparative example 1, indicating that the feeding method of the present invention can significantly improve the feathering rate.
Spawning condition: in test example 1, the number of eggs laid was as high as 46.51 eggs/female, and the shape of the eggs was full, and the number of eggs laid and the quality of the eggs were significantly higher than in comparative example 1.
TABLE 1 incubation of aphidicollis
Death of 21, 2 and 3 instar larvae in table
TABLE 3 pupation, eclosion and oviposition
The above embodiments are preferred embodiments of the present invention, but the present invention is not limited to the above embodiments, and any other changes, modifications, substitutions, combinations, and simplifications which do not depart from the spirit and principle of the present invention should be construed as equivalents thereof, and all such changes, modifications, substitutions, combinations, and simplifications are intended to be included in the scope of the present invention.
Claims (10)
1. The aphid eating gall midge feed is characterized by being prepared from the following raw materials in parts by weight:
200-300 parts of aphid juice, 50-100 parts of wheat germ juice, 200-300 parts of barley seedling juice, 10-30 parts of cane sugar and 0.02-0.05 part of food preservative;
the aphid juice is prepared by the following method:
1) and (3) cultivating clean seedlings:
selecting barley seeds without pesticide residues, soaking the seeds for 8-12 hours, planting the barley, and cultivating clean seedlings;
2) breeding aphids:
breeding aphids by using clean seedlings for 48-72 h;
3) cultivating aphids on an intermittent shaking table:
culturing the aphids bred in the step 2) by adopting an intermittent shaking table, wherein the culturing time is 12-24 h, the shaking time is 3-6 s each time, the pause time is 60-120 s, and the amplitude is 10-20 mm;
4) collecting aphids and secreted substances:
collecting the aphids cultivated in the step 3) and substances secreted by the aphids, pulping the aphids, and filtering to obtain an aphid supernatant; and (3) fully dissolving the secreted substances by adopting aphid supernatant to obtain aphid juice.
2. The aphid eating gall midge feed according to claim 1, wherein in step 2), the aphids are corn aphids and/or corn aphids.
3. The aphidicoling mosquito feed of claim 1, wherein said barley seedling juice is prepared by the following method:
pulping and filtering the clean seedlings to obtain barley seedling juice.
4. The aphidicollis feeding stuff according to claim 1, characterized in that the wheat germ juice is obtained by beating wheat germ and filtering.
5. A process for preparing an aphidicolin feeding fodder according to any one of claims 1 to 4, characterized in that it comprises the following steps:
firstly, adding cane sugar and a food preservative into barley seedling juice for full dissolution, and then uniformly mixing the barley seedling juice, the aphid juice and the wheat germ juice to obtain the aphid eating gall midge feed.
6. Use of an aphidicolor feeding feed as claimed in any of claims 1 to 4 for feeding aphidicolor.
7. A method for feeding aphid-eating gall midge, which comprises feeding aphid-eating gall midge by using the aphid-eating gall midge feed according to any one of claims 1 to 4.
8. The method for feeding aphidicoling mosquitoes as claimed in claim 7, which is characterized by comprising the following steps:
(1) pouring the aphid eating gall midge feed into a feeding disc, placing a wire mesh on the feeding disc, and then moving the feeding disc into an egg receiving chamber;
(2) inoculating the aphid eating cecidomyiia imago into an egg inoculating chamber, and mating and inoculating eggs;
(3) moving the feeding plate with the eggs into an insect room, moving the barley seedlings with the aphids into the insect room when the larvae are 2 years old until the larvae grow to be mature in the 6 th to 7 th days, and moving the barley seedlings out of the insect room;
(4) and (3) inclining the aged larvae into water, jumping the aged larvae into the water, collecting the aged larvae in the water by using a suction pipe, and transferring the collected aged larvae into a pupation bottle.
9. The method for feeding aphidicolin-feeding mosquitoes as claimed in claim 8, wherein in the step (2), the mating and egg receiving time is 24h in total.
10. The method for raising aphidicolis eating mosquito according to claim 8, characterized in that in step (3), the insect room is removed until 7d develops to an aged larva.
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