CN113207616A

CN113207616A - Culture medium and preparation method thereof, and culture medium structure and use method thereof

Info

Publication number: CN113207616A
Application number: CN202010080927.6A
Authority: CN
Inventors: 陈汶杨; 陈焕文; 蔡文清
Original assignee: Yuanxian Agricultural Biotechnology Co ltd
Current assignee: Yuanxian Agricultural Biotechnology Co ltd
Priority date: 2020-02-05
Filing date: 2020-02-05
Publication date: 2021-08-06

Abstract

The invention relates to a culture medium at least comprising a vegetable gum composed of a plant growth nutrient, vegetable powder and water, wherein the total weight of the vegetable gum is 100wt%, and a preparation method of the culture medium, and the invention also provides a culture medium layer of the culture medium, wherein the culture medium structure comprises the culture medium; a seedling transplanting jig plate capable of containing the culture medium layer, wherein the seedling transplanting jig plate comprises a hollow body, the hollow body is provided with an upper end hole and a lower end hole opposite to the other end of the upper end hole, and a top seat arranged on the periphery of the top end of the hollow body, the hollow body and the top seat are integrally formed, and the culture medium structure using method. The invention improves the growth health condition and quality of the plant crops by the culture medium of the solid agar containing the plant growth nutrient.

Description

Culture medium and preparation method thereof, and culture medium structure and use method thereof

Technical Field

The invention relates to a culture medium and a preparation method thereof, a culture medium structure and a use method thereof, in particular to a solid agar-agar culture medium containing plant growth nutrients and a preparation method thereof, and a culture medium structure and a use method thereof.

Background

According to the fact that the global greenhouse effect is more and more intensified along with the excessive industrial development of human beings and the drastic change of climate, the traditional mode of planting crops in soil is easily affected by the change of natural environment and the instability of climate, and the traditional soil cultivation is difficult to meet the quality requirement of human beings on the planted crops due to unexpected natural disasters, insect pests and artificial environmental pollution.

Therefore, in order to improve the conventional disadvantages of the conventional soil cultivation, the hydroponic cultivation oil is natural, as shown in fig. 1, the general hydroponic cultivation places a sponge (a) in a containing tank (C) containing a nutrient solution (B), and then plants a plant seed (D) on the sponge (a), since the hydroponic cultivation does not require soil, not only can get rid of the requirement limitation of the conventional agriculture on agricultural land, but also can avoid unexpected natural disasters, insect pests, and artificial environmental pollution problems, etc. compared with the conventional soil cultivation, in recent years, with the rising of environmental awareness, the use of disposable plastic wastes is gradually reduced, however, in this planting manner, when the plant is grown, the sponge is often damaged by the growing roots, resulting in the generation of a large amount of sponge which can not be reused after the plant is harvested, and, when the sponge is used as a substrate for planting crops, the roots of the planted crops are often pressed by the sponge, so that the growth of the roots and the roots are limited, the growth health condition of the planted crops is influenced, in addition, chemical components or salts are continuously accumulated in the nutrient solution used for hydroponics, and the chemical components and the salts with too high concentration can be harmful to the growth of plants.

Therefore, by means of the innovative hardware design, the problems of the conventional hydroponic cultivation using the foam that affects the growth of the roots of the planted crops, the generation of a large amount of non-recyclable sponge after the planted crops are harvested, and the situation that the chemical components or salts accumulated continuously are harmful to the growth of the planted crops are effectively improved, and the related industrial developers and related researchers need to continuously try to overcome and solve the problems.

Disclosure of Invention

Therefore, the present invention provides a culture medium and a method for preparing the same, and a culture medium structure and a method for using the same. Through the culture medium containing the solid agar containing the plant growth nutrient and the preparation method thereof, the nutrient solution used in hydroponic cultivation can be prevented from being continuously accumulated to influence the growth health of the planted crops, and the culture medium structure using the seedling transplanting jig plate and the use method thereof can still repeatedly utilize the seedling transplanting jig plate after the planted crops are harvested so as to reduce the cost of planting the planted crops each time.

The technical means adopted by the invention are as follows.

The main object of the present invention is to provide a culture medium, which at least comprises a vegetable gum composed of a plant growth nutrient, a vegetable powder and water, wherein the total weight of the vegetable gum is 100 wt%.

In one embodiment of the present invention, the plant growth nutrient is composed of a combination of diammonium phosphate and potassium nitrate.

In a first embodiment of the invention, the agar gum comprises from 0.5 to 2.5wt% of the agar powder.

In the first embodiment of the present invention, the amount of diammonium phosphate is 0.01 to 0.02 wt%.

In the first embodiment of the present invention, the potassium nitrate is 0.05 to 0.1 wt%.

In a second embodiment of the invention, the agar gum comprises 1wt% of the agar powder.

In a second embodiment of the invention, the diammonium phosphate is present in an amount of 0.01 to 0.02% by weight.

In the second embodiment of the present invention, the potassium nitrate is 0.05 to 0.1 wt%.

In another embodiment of the present invention, the plant growth nutrient is selected from at least one or more of diammonium phosphate, potassium nitrate, calcium nitrate, magnesium sulfate, manganese chloride, ferrous sulfate, boric acid, zinc sulfate, copper sulfate, ammonium molybdate and silicon dioxide.

In a third embodiment of the present invention, the agar gum comprises from 0.5 to 2.5wt% of the agar powder.

In the third embodiment of the present invention, the amount of diammonium phosphate is 0.01 to 0.02 wt%.

In the third embodiment of the present invention, the potassium nitrate is 0.05 to 0.1 wt%.

In the third embodiment of the present invention, the calcium nitrate is 0.05 to 0.1 wt%.

In a third embodiment of the present invention, the magnesium sulfate is 0.03 to 0.05 wt%.

In a third embodiment of the present invention, the manganese chloride is present in an amount of 0.00015 to 0.0003 wt%.

In the third embodiment of the present invention, the ferrous sulfate is 0.002-0.004 wt%.

In a third embodiment of the present invention, the boric acid is 0.0001 to 0.0003 wt%.

In the third embodiment of the present invention, the zinc sulfate is 0.00002 to 0.00004 wt%.

In a third embodiment of the present invention, the copper sulfate is 0.00002 to 0.00004 wt%.

In a third embodiment of the invention, the ammonium molybdate is 0.000001 to 0.000002 wt%.

In a third embodiment of the present invention, the silica is present in an amount of 0.00001 to 0.00002 wt%.

In a fourth embodiment of the invention, the agar gum comprises 1wt% of the agar powder.

In the fourth embodiment of the present invention, the amount of diammonium phosphate is 0.01 to 0.02 wt%.

In the fourth embodiment of the present invention, the potassium nitrate is 0.05 to 0.1 wt%.

In the fourth embodiment of the present invention, the calcium nitrate is 0.05 to 0.1 wt%.

In the fourth embodiment of the present invention, the magnesium sulfate is 0.03 to 0.05 wt%.

In a fourth embodiment of the present invention, the manganese chloride is present in an amount of 0.00015 to 0.0003 wt%.

In the fourth embodiment of the present invention, the ferrous sulfate is 0.002 to 0.004 wt%.

In a fourth embodiment of the present invention, the boric acid is 0.0001 to 0.0003 wt%.

In the fourth embodiment of the invention, the zinc sulfate is 0.00002 to 0.00004 wt%.

In a fourth embodiment of the present invention, the copper sulfate is 0.00002 to 0.00004 wt%.

In a fourth embodiment of the invention, the ammonium molybdate is 0.000001 to 0.000002 wt%.

In a fourth embodiment of the present invention, the silica is present in an amount of 0.00001 to 0.00002 wt%.

The main object of the present invention is to provide a method for preparing a culture medium, which at least comprises the following steps of mixing and dissolving a plant growth nutrient in water to form a plant growth nutrient solution; adjusting the pH value of the plant growth nutrient solution to 6.0; adding a vegetable powder to the aqueous plant growth nutrient solution and heating to dissolve the vegetable powder to form a vegetable solution; preparing a seedling transplanting jig plate and placing the seedling transplanting jig plate on a freezing plate with a solidification temperature; and adding the agar solution into the seedling transplanting jig plate with a preset volume, and cooling the agar solution to a cooling temperature to form agar.

In one embodiment of the present invention, the predetermined volume is 0.5 ml.

In one embodiment of the present invention, the cooling temperature is 50 ℃.

In one embodiment of the invention, the solidification temperature is-10 ℃ to 10 ℃.

The main object of the present invention is to provide a culture medium structure, comprising a culture medium layer of the culture medium as described above; a seedling transplanting jig plate capable of accommodating the culture medium layer, wherein the seedling transplanting jig plate comprises a hollow body, the hollow body is provided with an upper end hole and a lower end hole opposite to the other end of the upper end hole, and a top seat arranged on the periphery of the top end of the hollow body, and the hollow body and the top seat are integrally formed.

In an embodiment of the present invention, the hollow body has a cylindrical structure with the same diameter as the upper end hole and the lower end hole respectively.

In an embodiment of the present invention, the hollow body has a conical structure tapering from the upper end hole to the lower end hole.

In an embodiment of the present invention, a protrusion is further annularly disposed on an inner periphery of the lower end hole.

In an embodiment of the present invention, the lower hole further includes a sealing member capable of sealing the lower hole, and the sealing member has a plurality of through holes.

In an embodiment of the present invention, an outer curved surface of the hollow body has an inclination angle with a horizontal plane, and the inclination angle is smaller than 90 degrees.

In one embodiment of the present invention, an outer curved surface of the hollow body has an inclination angle with a horizontal plane, and the inclination angle is 77 degrees.

In an embodiment of the present invention, the water-retaining device further includes a water-retaining film covering the upper hole.

In an embodiment of the present invention, the container further includes a water retention film covering the upper hole and the top seat.

In an embodiment of the present invention, the present invention further comprises a planting frame having a plurality of planting holes.

In an embodiment of the present invention, the planting frame can be assembled on a planting water tank.

In one embodiment of the present invention, the planting water tank can be filled with a liquid.

The main objective of the present invention is to provide a method for using a culture medium structure, which at least comprises the following steps of taking a culture medium structure as described in an embodiment of the present invention; placing a vegetable seed on an upper surface of the culture medium layer; and placing the culture medium structure on a planting water tray.

In one embodiment of the invention, the vegetable seed may be a lettuce or cruciferae.

Therefore, the invention can prevent nutrient solution used in hydroponics from continuously accumulating to influence the growth health of the planted crops through the culture medium of the solid agar containing the plant growth nutrient and the preparation method thereof, and can repeatedly utilize the seedling transplanting jig plate after the planted crops are harvested by using the culture medium structure of the seedling transplanting jig plate and the use method thereof so as to reduce the cost of planting the planted crops each time.

Drawings

FIG. 1: conventional hydroponic status diagrams.

FIG. 2: the invention discloses an overall structure diagram of a seedling transplanting jig plate in a preferred embodiment.

FIG. 3A: the inclination angle of the seedling transplanting jig plate in a preferred embodiment of the invention is schematically shown.

FIG. 3B: the inclination angle of the seedling transplanting jig plate in a preferred embodiment of the invention is schematically shown.

FIG. 3C: the inclination angle of the seedling transplanting jig plate in a preferred embodiment of the invention is schematically shown.

FIG. 4: the flow chart of the method for preparing the culture medium according to the preferred embodiment of the invention is shown in the first embodiment.

FIG. 5: the flow chart of the preparation method of the culture medium of the preferred embodiment of the invention is shown in the second step.

FIG. 6: the flow chart of the preparation method of the culture medium of the preferred embodiment of the invention is (III).

FIG. 7: the structure of the culture medium of a preferred embodiment of the invention is schematically shown.

FIG. 8: the method for using the culture medium structure according to a preferred embodiment of the present invention is schematically shown in (a).

FIG. 9: the schematic diagram (II) of the method for using the culture medium structure of the preferred embodiment of the invention.

FIG. 10: the schematic diagram (III) of the method for using the culture medium structure of the preferred embodiment of the invention.

FIG. 11: the present invention provides a flow chart of a method for preparing a culture medium according to a preferred embodiment of the present invention.

FIG. 12: the flow chart of the method for using the culture medium structure of the preferred embodiment of the invention.

FIG. 13: the method for preparing a culture medium according to a preferred embodiment of the present invention is a linear graph of the freezing temperature of the freezing plate and the freezing time thereof required for freezing the culture medium.

FIG. 14: the present invention is a schematic diagram comparing the germination rates of seeds using sponge and agar according to the method of using the culture medium structure of a preferred embodiment of the present invention.

FIG. 15: the method of using the medium structure of the preferred embodiment of the present invention is a schematic diagram comparing the average growth heights of plants using sponge and agar.

FIG. 16: the present invention is a schematic diagram comparing the growth aberration rates of the sponge and the agar in the method of using the culture medium structure of a preferred embodiment of the present invention.

Description of the figure numbers:

in the prior art

Sponge A

B nutrient solution

C containing groove

D planting crop seeds

The invention

1 transplanting seedling jig plate

10 hollow body

101 external surface curved surface

11 upper end hole

12 lower end hole

121 projection

122 seal

1221 through hole

13 top seat

P horizontal plane

Angle of inclination theta

2 culture substrate layer

21 upper surface of the container

3 Water-retaining film

4 agar solution

41 agar-agar jelly

5 vegetable seeds

6 freezing plate

S10 to S14

S20 to S22.

Detailed Description

The main objective of the present invention is to provide a culture medium, which at least comprises a agar 41 composed of a plant growth nutrient, agar powder and water, wherein the plant growth nutrient, the agar powder and water are mixed and heated to dissolve the agar 41, the plant growth nutrient is a nutrient component required for plant growth, the plant growth nutrient is composed of a diammonium phosphate and a potassium nitrate, and the agar 41 contains 0.5-2.5wt% of the agar powder, wherein in an embodiment of the present invention, the plant growth nutrient can be 0.01-0.02wt% of the diammonium phosphate or 0.05-0.1wt% of the potassium nitrate. In one embodiment of the present invention, the agar glue 41 comprises 1wt% of the agar powder. In practice, the ratio of the plant growth nutrients and the agar 41 to be added can be adjusted according to the growth conditions of the plant to be planted or the size of the container for holding the culture medium.

The main objective of the present invention is to provide a culture medium, which at least comprises a agar 41 composed of a plant growth nutrient, agar powder and water, wherein the plant growth nutrient, the agar powder and water are mixed and heated to dissolve the agar 41 when the total weight of the agar 41 is 100wt%, and then cooled to form the agar 41, the plant growth nutrient is a nutrient component required for plant growth, and is selected from a group consisting of diammonium phosphate, potassium nitrate, calcium nitrate, magnesium sulfate, manganese chloride, ferrous sulfate, boric acid, zinc sulfate, copper sulfate, ammonium molybdate, silicon dioxide and any combination thereof Respiration, chlorophyll formation, nitrogen fixation, root and stem growth, and increased plant hardness to improve pest resistance or flowering and fruiting capacity, and the agar 41 comprises 0.5-2.5wt% of the agar powder, wherein in one embodiment of the invention, the plant growth nutrient can be 0.01-0.02wt% of diammonium phosphate, 0.05-0.1wt% of potassium nitrate, 0.05-0.1wt% of calcium nitrate, 0.03-0.05wt% of magnesium sulfate, 0.00015-0.0003wt% of manganese chloride, 0.002-0.004wt% of ferrous sulfate, 0.0001-0.0003wt% of boric acid, 0.00002-0.00004wt% of zinc sulfate, 0.00002-0.00004wt% of copper sulfate, 0.000001-0.000002wt% of ammonium molybdate, 0.00001-0.00002wt% of silicon dioxide, or a combination of at least one of the two. In one embodiment of the present invention, the agar glue 41 comprises 1wt% of the agar powder. In practice, the ratio of the plant growth nutrients and the agar 41 to be added can be adjusted according to the growth conditions of the plant to be planted or the size of the container for holding the culture medium, so as to promote the specific growth capacity of the plant, such as photosynthesis, respiration, chlorophyll formation, nitrogen fixation, growth of roots, stems and leaves, and increase the hardness of plant bodies to promote pest and disease resistance or to promote flowering and fruiting.

First, please refer to fig. 2 to 12 together, which are a schematic diagram of an overall structure of a seedling transplanting jig plate, a schematic diagram of an inclination angle of the seedling transplanting jig plate, a schematic diagram of a method for preparing a culture medium (i), a schematic diagram (ii), a schematic diagram (iii), a schematic diagram of a structure of the culture medium, a schematic diagram (i), a schematic diagram (ii), a schematic diagram (iii), a schematic diagram of a method for preparing the culture medium, and a schematic diagram of a method for using the culture medium according to a preferred embodiment of the present invention. The present invention also provides a method for preparing a culture medium, comprising the steps of (S10) dissolving a plant growth nutrient in water to form a plant growth nutrient solution, weighing the required plant growth nutrient selected from a group consisting of diammonium phosphate, potassium nitrate, calcium nitrate, magnesium sulfate, manganese chloride, ferrous sulfate, boric acid, zinc sulfate, copper sulfate, ammonium molybdate, silica and any combination thereof, pouring the plant growth nutrient into a container containing water, stirring until the plant growth nutrient is uniformly dissolved, and adding water to 100wt% in accordance with the required weight percentage of the plant growth nutrient; step S11, adjusting the pH value of the plant growth nutrient solution to 6.0 to avoid the situation that chemical precipitation or uneven dissolution is caused when the pH value of the plant growth nutrient in the plant growth nutrient solution is too high or too low so as to influence the fertility; step S12 adding a agar powder to the aqueous plant growth nutrient solution and heating to dissolve it to form an agar solution 4, in practice, adding the agar powder to the aqueous plant growth nutrient solution while heating and stirring until it dissolves uniformly to form an agar solution 4; step S13, preparing a seedling transplanting jig plate 1 to be placed on a freezing plate 6 with a solidification temperature, wherein the seedling transplanting jig plate 1 can be placed on the freezing plate 6 in advance for subsequent operation; step S14, adding the agar solution 4 to the transplanting jig plate 1 in a predetermined volume, wherein the agar solution 4 is pre-solidified on the surface of the agar solution 4 contacting the freezing plate 6 to form an agar 41, removing the transplanting jig plate 1 from the freezing plate 6, and cooling the agar solution 4 to a cooling temperature to form an agar 41, and wherein the agar solution 4 is added to the transplanting jig plate 1 in a predetermined volume according to the size of the transplanting jig plate 1, and the agar solution 4 is cooled by the freezing plate 6 to form a solid agar 41. In one embodiment of the present invention, the predetermined volume is 0.5ml, and the predetermined volume of the agar solution 4 to be added may be adjusted according to the growth of the plant to be planted or the size of the container to be filled with the culture medium. In one embodiment of the present invention, the freezing temperature is-10 ℃ to 10 ℃, and in practice, the freezing plate 6 can penetrate the freezing temperature of-10 ℃ to 10 ℃ in advance, and the surface of the agar solution 4 contacting the freezing plate 6 is pre-solidified to form agar 41. In one embodiment of the present invention, the cooling temperature is 50 ℃, and in practice, the cooling temperature can be adjusted to 50 ℃ to cool and solidify the agar solution 4 to form agar 41 with good quality.

According to the object of the present invention, the present inventors propose a method for preparing a culture medium, which can also be sequentially performed by the steps of S10 mixing and dissolving a plant growth nutrient in water to form a plant growth nutrient solution; step S11, adjusting the pH value of the plant growth nutrient solution to 6.0; step S12 adding a vegetable powder to the plant growth nutrient solution and heating to dissolve to form a vegetable solution 4; step S13, preparing a seedling transplanting jig plate 1 to be placed on a freezing plate 6 with a solidification temperature; step S14, adding the agar solution 4 to the transplanting jig plate 1 in a predetermined volume, and cooling the agar solution 4 to a cooling temperature to form an agar 41.

The present invention further provides a culture medium structure, comprising a culture medium layer 2 as described above, a seedling-transplanting jig plate 1 for accommodating the culture medium layer 2, wherein the culture medium layer 2 can be pre-formed in the seedling-transplanting jig plate 1 or the formed culture medium layer 2 can be added into the seedling-transplanting jig plate 1, the seedling-transplanting jig plate 1 comprises a hollow body 10, the hollow body 10 has an upper end hole 11 and a lower end hole 12 opposite to the other end of the upper end hole 11, in practice, the culture medium layer 2 can not slide out of the hollow body 10 through the hollow body 10, the agar solution 4 can be added into the seedling-transplanting jig plate 1 through the upper end hole 11, the upper end hole 11 can allow plants to grow upwards, the roots of the plants can pass through the lower end hole 12, and a top seat 13 disposed at the top end of the hollow body 10, the hollow body 10 is integrally formed with the top base 13. In one embodiment of the present invention, the hollow body 10 has a cylindrical structure with the same diameter of the upper end hole 11 and the lower end hole 12; the hollow body 10 is a cone structure tapering from the upper end hole 11 to the lower end hole 12, the cylindrical structure or the cone structure of the hollow body 10 is further provided with a protrusion 121 through the inner periphery of the lower end hole 12 for bearing the culture medium layer 2, so that the culture medium layer 2 will not slide out of the hollow body 10; or the lower end hole 12 further comprises a sealing member 122 for sealing the lower end hole 12 so that the culture medium layer 2 does not slide out of the hollow body 10, and the sealing member 122 has a plurality of through holes 1221 for allowing the roots of the plants to pass through the through holes 1221 of the lower end hole 12 so that the roots of the plants can pass through the lower end hole 12 and absorb nutrients. In an embodiment of the present invention, an outer curved surface 101 of the hollow body 10 and a horizontal plane P have an inclination angle θ, and the inclination angle θ is smaller than 90 degrees; or an outer curved surface 101 of the hollow body 10 has an inclination angle theta with a horizontal plane P, the inclination angle theta is 77 degrees, in practice, the culture medium layer 2 will not slide out of the hollow body 10 through the outer curved surface 101 of the hollow body 10 and the horizontal plane P having an inclination angle theta. In an embodiment of the present invention, the present invention further includes a water retention film 3, wherein the water retention film 3 covers the upper hole 11; or further comprises a water retention film 3, the water retention film 3 is covered on the upper end hole 11 and the top seat 13, and the water retention film 3 can be used for keeping the water in the culture medium layer 2 of the culture medium structure to evaporate. In an embodiment of the present invention, the seedling planting jig further comprises a planting rack having a plurality of planting holes, the top base 13 of the seedling planting jig plate 1 can be engaged with the planting holes, and the planting rack can be assembled on a planting water tank for placing a liquid so that the roots of the planted plants can pass through the lower end holes 12 and absorb the liquid.

The present invention also provides a method for using a culture medium structure, comprising the steps of S20 taking a culture medium structure as described in an embodiment of the present invention; step S21 placing a vegetable seed 5 on an upper surface 21 of the culture medium layer 2; step S22 is to place the culture medium structure on a planting water tank, wherein the planting water tank can be pre-filled with a liquid or filled with a liquid after the vegetable seeds 5 germinate, when the vegetable seeds 5 germinate, the upper hole 11 allows the plants to grow upwards, and the roots of the plants can pass through the lower hole 12 and absorb the liquid on the planting water tank. In one embodiment of the present invention, the vegetable seed 5 may be a lettuce or cruciferae. Next, to further illustrate the objects, features and effects of the present invention, one of the practical embodiments of the method for using the medium structure of the present invention is described below to further demonstrate the practical application range of the method for using the medium structure of the present invention, but the scope of the present invention is not intended to be limited in any way. According to the object of the present invention, the present inventors propose a method for using a culture medium structure, which can also sequentially perform the following steps, step S20 taking a culture medium structure; step S21 placing a vegetable seed 5 on an upper surface 21 of the culture medium layer 2; step S22, placing the culture medium structure on a water planting tray.

In order to make the disclosure more complete and complete, the following description is given for illustrative purposes, with reference to embodiments and examples of the invention; it is not intended to be the only form in which the embodiments of the invention may be practiced or utilized.

FIG. 13 is a graph showing the linear relationship between the solidification time and the solidification temperature of the culture medium according to the preferred embodiment of the present invention.

< culture Medium solidification time and solidification temperature test in culture Medium preparation method >

The freezing temperature of the freezing plate and the freezing time thereof required for the medium to freeze were evaluated.

The test method comprises the following steps:

mixing and dissolving a plant growth nutrient in water to form a plant growth nutrient solution; adjusting the pH value of the plant growth nutrient solution to 6.0; adding a vegetable powder to the aqueous plant growth nutrient solution and heating to dissolve the vegetable powder to form a vegetable solution; preparing a seedling transplanting jig plate and placing the seedling transplanting jig plate on a freezing plate with a solidification temperature; and adding 0.5ml of the agar solution to the transplanting jig plate, and cooling the agar solution to 50 ℃ to form agar, wherein the solidification temperature of different freezing plates is tested, and the solidification time of the agar solution contacting the surface of the freezing plate is recorded.

Results and discussion:

referring again to FIG. 13, a linear plot of medium freezing time versus temperature is shown for the medium preparation method, with the X-axis representing different freezing plate freezing temperatures and the Y-axis representing freezing time. The test was conducted by taking the freezing temperatures of the freezing plates at-10 deg.C, 5 deg.C, 0 deg.C, 5 deg.C and 10 deg.C, respectively, and as shown in FIG. 12, the results were obtained when the freezing temperature of the freezing plate at-10 deg.C, 5 deg.C or 0 deg.C was about 10 seconds, the freezing temperature of the freezing plate at 5 deg.C was about 20 seconds, and the freezing temperature of the freezing plate at 10 deg.C was about 30 seconds, and R in the linear regression analysis thereof²The value of 0.7813 can be analyzed by test results to determine the freezing temperature of the frozen plate at-10 deg.C, 5 deg.C or 0 deg.C, and less time is required to pre-solidify the surface of the agar solution contacted with the frozen plate to form an agar gel. Therefore, according to the culture medium solidification time and solidification temperature test results of the culture medium preparation method, the culture medium of the culture medium preparation method can penetrate through a freezing plate, the time for forming agar by pre-solidifying the agar solution surface is shortened, and the production efficiency of the whole agar preparation can be improved when the culture medium is prepared on a large scale and the agar quality of the culture medium is not influenced.

Referring to FIG. 14, a diagram of seed germination rates of sponge and agar according to a preferred embodiment of the present invention is shown.

< seed germination percentage test Using sponge and agar >

Seed germination rates using sponges and agar were evaluated.

The test method comprises the following steps:

taking a medium structure as described in an embodiment of the present invention; placing a vegetable seed on an upper surface of the culture medium layer; and placing the culture medium structure on a planting water tray, placing a vegetable seed on the sponge, simultaneously placing the sponge on the planting water tray, respectively selecting 100 pieces of the red lettuce and the kale, culturing for 3 days, and observing the seed germination rates of the red lettuce and the kale.

Results and discussion:

referring again to fig. 14, a graph comparing the germination rates of seeds using sponge and agar is shown, wherein X-axis represents the vegetable seeds of red lettuce and kale and the germination percentage of sponge and agar. When the vegetable seeds are red lettuce rolls and the sponge and the agar are respectively used for culturing, the germination rate of the red lettuce rolls cultured by the agar is almost 99 percent, while the germination rate of the red lettuce rolls cultured by the sponge is only about 80 percent, and when the vegetable seeds are collard and the sponge and the agar are respectively used for culturing, the germination rate of the collard cultured by the agar is almost 99 percent, while the germination rate of the collard cultured by the sponge is only about 80 percent, and the analysis of the overall germination rate shows that the vegetable seeds cultured by the agar have higher seed germination rate compared with the vegetable seeds cultured by the sponge. Therefore, from the results of the seed germination rate tests using the sponge and the agar, the method for using the culture medium structure of the invention can improve the whole seed germination rate by culturing the vegetable seeds through the agar, and can improve the seed germination rate of the whole vegetable seeds when culturing the vegetable seeds on a large scale, so as to greatly reduce the condition that the seeds do not germinate, and further improve the vegetable production rate.

Referring to fig. 15, a schematic diagram of a comparison of average growth heights of planted plants using sponge and agar according to a method of using the culture medium structure of a preferred embodiment of the present invention is shown.

< average growth height test of planted crop Using sponge and agar >

The average growth height of the planted crops using the sponge and the agar was evaluated.

The test method comprises the following steps:

taking a medium structure as described in an embodiment of the present invention; placing a vegetable seed on an upper surface of the culture medium layer; and placing the culture medium structure on a planting water tray, placing a vegetable seed on the sponge, placing the sponge on the planting water tray, respectively selecting 100 pieces of red lettuce and collard for the vegetable seed, culturing for 21 days, observing the average growth height of the planted crops of the red lettuce and the collard, and measuring the average growth height of the planted crops from the upper surface of the culture medium layer to the highest point of the planted crops.

Results and discussion:

referring to FIG. 15, the average growth height of plants using sponge and agar is shown, wherein X-axis represents the vegetable seeds of Lactuca sativa and Brassica oleracea, respectively, and the average growth height of plants using sponge and agar, respectively. When the vegetable seeds were red lettuce rolls and they were cultured with sponge and agar, respectively, it was observed that the average growth height of red lettuce rolls cultured with agar was about 9.7cm and the average growth height of red lettuce rolls cultured with sponge was about 5.8cm, whereas when the vegetable seeds were kale and they were cultured with sponge and agar, respectively, it was observed that the average growth height of kale cultured with agar was about 18.7cm and the average growth height of kale cultured with sponge was about 10.2cm, and the average growth height analysis of the whole crop plants revealed that the vegetable seeds cultured with agar had a higher average growth height than the vegetable seeds cultured with sponge. Therefore, the result of the average growth height test of the planted crops using the sponge and the agar shows that the application method of the culture medium structure can improve the average growth height of the whole planted crops by culturing the vegetable seeds through the agar, and can improve the average growth height of the whole planted crops when the planted crops are cultured in a large scale so as to greatly increase the production weight of the vegetable production.

Referring to FIG. 16, a diagram illustrating the growth aberration rate of agar and sponge according to the method for using the culture medium structure of a preferred embodiment of the present invention is shown.

< comparative test of growth distortion Using sponge and agar >

The growth deformity rates using sponges and agar were evaluated.

The test method comprises the following steps:

taking a medium structure as described in an embodiment of the present invention; placing a vegetable seed on an upper surface of the culture medium layer; and placing the culture medium structure on a planting water tray, placing a vegetable seed on the sponge, and simultaneously placing the sponge on the planting water tray, wherein the vegetable seed is 100 vegetable seeds of red-roll lettuce, and observing the growth deformity rate of the planted crop after culturing for 21 days, and the growth deformity of the planted crop is defined as that the grown and deformed planted crop has abnormal variation relative to the normally grown planted crop.

Results and discussion:

please refer to the graph, which shows the comparison of the growth deformity rate of Lactuca sativa and agar, wherein the X-axis represents the case of Lactuca sativa with sponge and agar, respectively, and the Y-axis represents the deformity rate of the plants. When the sponge culture is used, the rate of deformity is almost 19%, whereas when the agar culture is used, the rate of deformity is only about 0.1, which can be analyzed from the test results, and there is a very low case where the agar culture is used as compared with the sponge culture, which causes growth deformity of the cultivated plant. Therefore, the result of the comparative experiment on the growth aberration rate of the sponge and the agar shows that the method for using the culture medium structure can improve the growth health condition of the whole planted crops by culturing the vegetable seeds through the agar, and can improve the production quality of the whole planted crops when the planted crops are cultured in a large scale.

In summary, compared with the prior art and the products, the present invention has one of the following advantages.

The invention aims to provide a solid agar gel containing plant growth nutrients, which maintains the fixed proportion of the plant growth nutrients through the solid agar gel and prevents the nutrient solution used in hydroponic culture from continuously accumulating.

The object of the present invention is to provide a harvesting jig plate which can be reused after harvesting of the plant crops, thereby reducing the cost of planting the plant crops each time.

The present invention is directed to a solid agar gel containing a plant growth nutrient, which is capable of being easily removed from a plant when the plant is harvested without affecting the integrity of the root of the plant.

The invention aims to produce healthy plant crops by using solid agar containing plant growth nutrients, which has higher germination rate and lower deformity rate compared with plastic foam used in traditional hydroponics.

Claims

1. A culture medium comprises at least one agar (41) comprising a plant growth nutrient, agar powder and water, wherein the total weight of the agar (41) is 100 wt%.

2. The culture medium of claim 1, wherein the plant growth nutrient is a combination of diammonium phosphate and potassium nitrate.

3. A culture medium according to claim 2, wherein said agar (41) comprises 0.5-2.5wt% of said agar powder.

4. The culture medium of claim 3, wherein the diammonium phosphate is 0.01-0.02 wt%.

5. The culture medium according to claim 3, wherein the potassium nitrate is 0.05-0.1 wt%.

6. A culture medium according to claim 2, wherein said agar (41) comprises 1wt% of said agar powder.

7. The culture medium of claim 6, wherein the diammonium phosphate is 0.01-0.02 wt%.

8. The culture medium according to claim 6, wherein the potassium nitrate is 0.05-0.1 wt%.

9. The culture medium of claim 1, wherein the plant growth nutrient is selected from the group consisting of at least one or more of diammonium phosphate, potassium nitrate, calcium nitrate, magnesium sulfate, manganese chloride, ferrous sulfate, boric acid, zinc sulfate, copper sulfate, ammonium molybdate, and silica.

10. A culture medium according to claim 9, wherein said agar (41) comprises 0.5-2.5wt% of said agar powder.

11. The culture medium of claim 10, wherein the diammonium phosphate is 0.01-0.02 wt%.

12. The culture medium according to claim 10, wherein the potassium nitrate is 0.05-0.1 wt%.

13. The culture medium of claim 10, wherein the calcium nitrate is 0.05-0.1 wt%.

14. The culture medium of claim 10, wherein the magnesium sulfate is 0.03-0.05 wt%.

15. The culture medium of claim 10, wherein the manganese chloride is 0.00015-0.0003 wt%.

16. The culture medium of claim 10, wherein the ferrous sulfate is 0.002-0.004 wt%.

17. The culture medium of claim 10, wherein the boric acid is 0.0001 to 0.0003 wt%.

18. The culture medium of claim 10, wherein the zinc sulfate is 0.00002 to 0.00004 wt%.

19. The culture medium of claim 10, wherein the copper sulfate is 0.00002 to 0.00004 wt%.

20. The culture medium of claim 10, wherein the ammonium molybdate is 0.000001 to 0.000002 wt%.

21. The culture medium of claim 10, wherein the silica is 0.00001 to 0.00002 wt%.

22. The culture medium of claim 9, wherein said agar (41) comprises 1wt% of said agar powder.

23. The culture medium of claim 22, wherein the diammonium phosphate is 0.01-0.02 wt%.

24. The culture medium of claim 22, wherein the potassium nitrate is 0.05-0.1 wt%.

25. The culture medium of claim 22, wherein the calcium nitrate is 0.05-0.1 wt%.

26. The culture medium of claim 22, wherein the magnesium sulfate is 0.03-0.05 wt%.

27. The culture medium of claim 22, wherein the manganese chloride is 0.00015-0.0003 wt%.

28. The culture medium of claim 22, wherein the ferrous sulfate is 0.002-0.004 wt%.

29. The culture medium of claim 22, wherein the boric acid is 0.0001 to 0.0003 wt%.

30. The culture medium of claim 22, wherein the zinc sulfate is 0.00002 to 0.00004 wt%.

31. The culture medium of claim 22, wherein the copper sulfate is 0.00002 to 0.00004 wt%.

32. The culture medium of claim 22, wherein the ammonium molybdate is 0.000001 to 0.000002 wt%.

33. The culture medium of claim 22, wherein the silica is 0.00001 to 0.00002 wt%.

34. A method for preparing a culture medium, comprising the steps of (S10) mixing a plant growth nutrient with water to form a plant growth nutrient solution; (S11) adjusting the pH of the plant growth nutrient solution to 6.0; (S12) adding a vegetable powder to the aqueous plant growth nutrient solution and heating to dissolve to form a vegetable solution (4); (S13) preparing a seedling transplanting jig plate (1) to be placed on a freezing plate (6) with a solidification temperature; (S14) adding the agar solution (4) to the transplanting jig plate (1) in a predetermined volume, and cooling the agar solution (4) to a cooling temperature to form an agar gel (41).

35. The method of claim 34, wherein the predetermined volume is 0.5 ml.

36. The method of claim 34, wherein the cooling temperature is 50 ℃.

37. The method of claim 34, wherein the freezing temperature is-10 ℃ to 10 ℃.

38. A media structure comprising a media layer (2) of a media according to claim 1; a seedling transplanting jig plate (1) capable of accommodating the culture medium layer (2), wherein the seedling transplanting jig plate (1) comprises a hollow body (10), the hollow body (10) is provided with an upper end hole (11), a lower end hole (12) opposite to the other end of the upper end hole (11), and a top seat (13) arranged on the periphery of the top end of the hollow body (10), and the hollow body (10) and the top seat (13) are integrally formed.

39. A culture medium structure according to claim 38, characterized in that the hollow body (10) has a cylindrical configuration with the upper end hole (11) and the lower end hole (12) having the same diameter, respectively.

40. A medium structure according to claim 38, characterized in that the hollow body (10) is a conical structure tapering from the upper end hole (11) to the lower end hole (12).

41. A culture medium structure according to claim 38, characterized in that the lower end hole (12) is provided with a protrusion (121) at its inner peripheral ring.

42. The media structure of claim 38, wherein the lower port (12) comprises a seal (122) for sealing the lower port (12), the seal (122) having a plurality of through holes (1221).

43. A culture medium structure according to claim 40, characterized in that an outer curved surface (101) of the hollow body (10) has an inclination angle (θ) with a horizontal plane (P), the inclination angle (θ) being less than 90 degrees.

44. A culture medium structure according to claim 40, characterized in that an outer curved surface (101) of the hollow body (10) has an inclination angle (θ) with a horizontal plane (P), the inclination angle (θ) being 77 degrees.

45. The culture medium structure of claim 38, comprising a water-retaining membrane (3), wherein the water-retaining membrane (3) is disposed over the upper hole (11).

46. The culture medium structure of claim 38, comprising a water-retaining membrane (3), wherein the water-retaining membrane (3) covers the upper hole (11) and the top seat (13).

47. The media structure of claim 38, comprising a planting frame having a plurality of planting holes.

48. The media structure of claim 47, wherein the planting frame is disposed on a planting water trough.

49. The media structure of claim 48, wherein the planting water trough is filled with a liquid.

50. A method of using a media structure, comprising the steps of (S20) taking a media structure of claim 38; (S21) placing a vegetable seed (5) on an upper surface (21) of the medium layer (2); (S22) placing the culture medium structure on a water planting plate.

51. Method for using a culture medium according to claim 50, characterized in that the vegetable seed (5) is of the lettuce or Brassicaceae family.