CN113207358A - Seed germination detection method in dark scene - Google Patents
Seed germination detection method in dark scene Download PDFInfo
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- CN113207358A CN113207358A CN202110632495.XA CN202110632495A CN113207358A CN 113207358 A CN113207358 A CN 113207358A CN 202110632495 A CN202110632495 A CN 202110632495A CN 113207358 A CN113207358 A CN 113207358A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
- A01C1/02—Germinating apparatus; Determining germination capacity of seeds or the like
- A01C1/025—Testing seeds for determining their viability or germination capacity
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Abstract
The invention discloses a seed germination detection method in a dark light scene, which comprises the steps of selecting 100 seeds, pretreating the 100 seeds to break the dormancy state, averagely dividing the 100 seeds into four groups, averagely dividing each group into five groups, and 5 seeds in each group, placing an incubator for seed germination in a dark light environment, arranging an oxygen supply device, a temperature control device, a humidity control device and an illumination control device, germinating the four groups of seeds, and measuring the vitality of the seeds by a tetrazole staining method. The method has the advantages of detecting the germination conditions of the seeds under the conditions of different temperatures, different humidity, different oxygen contents and different illumination, and detecting more comprehensively.
Description
Technical Field
The invention relates to the technical field of seed germination detection, in particular to a seed germination detection method in a dark light scene.
Background
Seeds are the unique propagules of gymnosperms and angiosperms, which are formed by the ovule undergoing pollination fertilization. The seeds generally consist of 3 parts of a seed coat, an embryo and an endosperm, and some mature seeds of plants only comprise two parts of the seed coat and the embryo. The formation of seeds allows the young sporozoite ovules to be protected maternal and to be nourished sufficiently like a mammalian fetus. Seeds also have various structures suitable for spreading or resisting adverse conditions, creating good conditions for the ethnic continuation of plants.
In some cases, the seeds enter a dormant state, which is a biological phenomenon that the seeds having vital signs cannot normally germinate according to time in the combination of environmental factors expected to be normal and beneficial to the germination of the seeds within a predetermined time range. Dormant seeds are broken into a dormant state before a seed germination experiment is carried out.
The seed germination test is considered to be the most convincing method for evaluating the harmless decomposition of the heap rot product. The germination index of the seeds comprehensively reflects the phytotoxicity of the compost and is considered as the most sensitive and reliable compost maturity evaluation index. Generally, a germination index of greater than 50% would be considered substantially non-toxic to the seeds.
The germination index is an indicator of the viability of the seed. During the germination test, the number of the germination grains is recorded every day, and then the germination index is calculated, so that the germination index is high, and the vitality is high.
The germination condition of the seeds can be determined by a seed germination experiment performed in a dark light scene, in a seed germination laboratory, the seeds are generally placed in the same environment for germination, the measured germination condition of the seeds can only be reflected in the set environment, and the influence of the temperature, humidity, oxygen content and illumination condition on the germination of the seeds cannot be obtained.
Disclosure of Invention
The invention aims to provide a seed germination detection method in a dark light scene, which has the advantages of detecting the germination conditions of seeds under the conditions of different temperatures, different humidities, different oxygen contents and different illumination, detecting more comprehensively, and solving the problem that the measured germination conditions of the seeds can only be reflected in a set environment and the influence of the temperature, humidity, oxygen contents and illumination conditions on the germination of the seeds can not be obtained when the seeds are placed in the same environment for germination.
In order to achieve the purpose, the invention provides the following technical scheme: a seed germination detection method in a dark light scene comprises the steps of selecting 100 seeds, pretreating the 100 seeds to break the dormant state, averagely dividing the 100 seeds into four groups, averagely dividing each group into five groups, and 5 seeds in each group, placing an incubator for seed germination in a dark light environment, arranging an oxygen supply device, a temperature control device, a humidity control device and a light control device, carrying out germination treatment on the four groups of seeds, and measuring the vitality of the seeds by a tetrazole staining method.
As a further aspect of the present invention, the low-temperature lamination pretreatment:
s1, soaking the selected 100 seed samples in clear water at the temperature of 5-10 ℃;
s2, adding the sphagna, the fine sand, the vermiculite and the peat soil into the pretreated container, and mixing the sphagna, the fine sand, the vermiculite and the peat soil in the container;
s3, burying the soaked seeds into a pretreatment container, wherein the seeds are separated by 3-5 cm;
s4, controlling the temperature inside the pretreated container to be between 5 and 10 ℃;
s5, taking the treated seeds out of the pre-treated container;
the dormancy state of the seeds is broken through a low-temperature stratification pretreatment mode.
As a further aspect of the invention, the preparation before detection is:
s1, wrapping 100 seed samples broken into dormancy states with dehydrated cotton, and absorbing moisture on the surfaces of the seeds through the dehydrated cotton;
s2, removing the skins of 100 seed samples;
s3, placing the incubator in a dark scene, and paving gauze inside the incubator;
s4, an oxygen supply device, a temperature control device, a humidity control device and a light control device are arranged at the incubator.
As a further scheme of the invention, the detection process comprises the following steps:
evenly dividing 100 seeds into four groups, wherein each group is evenly divided into five groups, and each group is 5 seeds;
a first group: respectively placing 5 seeds in the five subgroups of the first large group on gauze of five incubators, keeping the oxygen content in the five incubators consistent through an oxygen supply device, keeping the humidity in the five incubators consistent through a humidity control device, keeping the light brightness in the five incubators consistent through a light control device, setting the temperature in the five incubators into five temperature areas through a temperature control device, and respectively keeping the temperature in the five incubators at 2 ℃ from low to high until the seeds germinate and then taking out;
second group: respectively placing 5 seeds in the five subgroups of the second main group on gauzes of five incubators, keeping the humidity in the five incubators consistent through a humidity control device, keeping the light brightness in the five incubators consistent through a lighting control device, keeping the temperature in the five incubators consistent through a temperature control device, setting the oxygen contents in the five incubators into five areas through an oxygen supply device, and taking out the seeds after the oxygen contents in the five incubators are respectively different by 2% from low to high until the seeds germinate;
third group: respectively placing 5 seeds in a fifth group of the third group on gauze of five incubators, keeping the oxygen content in the five incubators consistent through an oxygen supply device, keeping the temperature in the five incubators consistent through a temperature control device, keeping the light brightness in the five incubators consistent through a light control device, setting the humidity in the five incubators into five areas through a humidity control device, and respectively keeping the difference of 2% between the humidity in the five incubators from low to high until the seeds germinate and then taking out the seeds;
and a fourth group: respectively placing 5 seeds in a fifth group of five groups of the fourth group on gauze of five incubators, keeping consistent oxygen content in the five incubators through an oxygen supply device, keeping consistent humidity in the five incubators through a humidity control device, keeping consistent temperature in the five incubators through a temperature control device, setting brightness in the five incubators into five areas through a light control device, and taking out the five incubators after the seeds germinate;
the four groups of germinated seeds are respectively subjected to tetrazolium staining method to determine the viability of the seeds, the germination conditions of the seeds under the conditions of different temperatures, different humidities, different oxygen contents and different illumination are detected, and the influence degree of the conditions of different temperatures, different humidities, different oxygen contents and different illumination on the germination rate of the seeds is determined.
Compared with the prior art, the invention has the following beneficial effects: evenly dividing 100 seeds into four groups, wherein each group is evenly divided into five groups, and each group is 5 seeds;
a first group: respectively placing 5 seeds in the five groups of the first large group on gauzes of five culture boxes, keeping consistent oxygen content, humidity and light brightness, respectively keeping consistent temperature difference of 2 ℃ from low to high in the five culture boxes, and taking out the seeds after the seeds germinate;
second group: respectively placing 5 seeds in the five groups of the second group on gauzes of five culture boxes, keeping the humidity consistent, the light brightness consistent and the temperature consistent, respectively keeping the difference of 2% between the oxygen contents in the five culture boxes from low to high, and taking out the seeds after the seeds germinate;
third group: respectively placing 5 seeds in the five groups of the third group on gauzes of five culture boxes, keeping consistent oxygen content, temperature and light brightness, respectively keeping consistent humidity difference of 2% from low to high in the five culture boxes, and taking out the seeds after germination;
and a fourth group: respectively placing 5 seeds in the five groups of the fourth group on gauzes of five culture boxes, keeping consistent oxygen content, humidity and temperature, keeping consistent brightness in the five culture boxes from low to high, and taking out the seeds after the seeds germinate;
the viability of the seeds was determined by tetrazolium staining of four groups of germinated seeds.
The germination conditions of the seeds under the conditions of different temperatures, different humidities, different oxygen contents and different illumination are detected, and the detection is more comprehensive.
Detailed Description
The technical solutions in the present invention will be described clearly and completely with reference to the following embodiments of the present invention, and it should be understood that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
In the description of the present invention, it should be noted that the terms "upper", "lower", "inner", "outer", "front", "rear", "both ends", "one end", "the other end", etc., indicate orientations or positional relationships based on the orientations or positional relationships shown herein, and are only for convenience of description and simplicity of description, but do not indicate or imply that the referred device or element must have a specific orientation, be constructed in a specific orientation, and be operated, and thus, should not be construed as limiting the present invention. Furthermore, the terms "first" and "second" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance.
In the description of the present invention, it is to be noted that, unless otherwise explicitly specified or limited, the terms "mounted," "disposed," "connected," and the like are to be construed broadly, such as "connected," which may be fixedly connected, detachably connected, or integrally connected; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meanings of the above terms in the present invention can be understood in specific cases to those skilled in the art.
Example 1
The embodiment provided by the invention comprises the following steps: a seed germination detection method in a dark light scene comprises the steps of selecting 100 seeds, pretreating the 100 seeds to break the dormant state, averagely dividing the 100 seeds into four groups, averagely dividing each group into five groups, and 5 seeds in each group, placing an incubator for seed germination in a dark light environment, arranging an oxygen supply device, a temperature control device, a humidity control device and a light control device, carrying out germination treatment on the four groups of seeds, and measuring the vitality of the seeds by a tetrazole staining method.
Example 2
The embodiment provided by the invention comprises the following steps: a seed germination detection method in a dark light scene comprises the following steps of low-temperature stratification pretreatment:
s1, soaking 100 selected seed samples in clear water at the temperature of 5-10 ℃, adjusting the temperature of the clear water according to different varieties of the seeds, and enabling the seeds to absorb water to be in a half-full state;
s2, adding the sphagna, the fine sand, the vermiculite and the peat soil into the pretreated container, and mixing the sphagna, the fine sand, the vermiculite and the peat soil in the container;
s3, burying the soaked seeds into a pretreatment container, wherein the seeds are separated by 3-5 cm, and enough space is reserved between the seeds to ensure the dormancy breaking effect of the seed pretreatment;
s4, controlling the internal temperature of the pretreated container to be between 5 and 10 ℃, and adjusting the internal temperature of the pretreated container according to different varieties of seeds;
s5, taking the treated seeds out of the pre-treated container;
the dormancy state of the seeds is broken through a low-temperature stratification pretreatment mode.
Example 3
The embodiment provided by the invention comprises the following steps: a seed germination detection method in a dark scene is prepared before detection:
s1, wrapping 100 seed samples broken into dormancy states with dehydrated cotton, and absorbing moisture on the surfaces of the seeds through the dehydrated cotton;
s2, removing the skins of 100 seed samples;
s3, placing the incubator in a dark scene, and paving gauze inside the incubator;
s4, an oxygen supply device, a temperature control device, a humidity control device and a light control device are arranged at the incubator.
Example 4
The embodiment provided by the invention comprises the following steps: a seed germination detection method in a dark light scene comprises the following detection processes:
evenly dividing 100 seeds into four groups, wherein each group is evenly divided into five groups, and each group is 5 seeds;
a first group: respectively placing 5 seeds in the five subgroups of the first large group on gauze of five incubators, keeping the oxygen content in the five incubators consistent through an oxygen supply device, keeping the humidity in the five incubators consistent through a humidity control device, keeping the light brightness in the five incubators consistent through a light control device, setting the temperature in the five incubators into five temperature areas through a temperature control device, and respectively keeping the temperature in the five incubators at 2 ℃ from low to high until the seeds germinate and then taking out;
second group: respectively placing 5 seeds in the five subgroups of the second main group on gauzes of five incubators, keeping the humidity in the five incubators consistent through a humidity control device, keeping the light brightness in the five incubators consistent through a lighting control device, keeping the temperature in the five incubators consistent through a temperature control device, setting the oxygen contents in the five incubators into five areas through an oxygen supply device, and taking out the seeds after the oxygen contents in the five incubators are respectively different by 2% from low to high until the seeds germinate;
third group: respectively placing 5 seeds in a fifth group of the third group on gauze of five incubators, keeping the oxygen content in the five incubators consistent through an oxygen supply device, keeping the temperature in the five incubators consistent through a temperature control device, keeping the light brightness in the five incubators consistent through a light control device, setting the humidity in the five incubators into five areas through a humidity control device, and respectively keeping the difference of 2% between the humidity in the five incubators from low to high until the seeds germinate and then taking out the seeds;
and a fourth group: respectively placing 5 seeds in a fifth group of five groups of the fourth group on gauze of five incubators, keeping consistent oxygen content in the five incubators through an oxygen supply device, keeping consistent humidity in the five incubators through a humidity control device, keeping consistent temperature in the five incubators through a temperature control device, setting brightness in the five incubators into five areas through a light control device, and taking out the five incubators after the seeds germinate;
the four groups of germinated seeds are respectively subjected to tetrazolium staining method to determine the viability of the seeds, the germination conditions of the seeds under the conditions of different temperatures, different humidities, different oxygen contents and different illumination are detected, and the influence degree of the conditions of different temperatures, different humidities, different oxygen contents and different illumination on the germination rate of the seeds is determined.
The germination conditions of the seeds under the conditions of different temperatures, different humidities, different oxygen contents and different illumination are detected, and the detection is more comprehensive.
It will be evident to those skilled in the art that the invention is not limited to the details of the foregoing illustrative embodiments, and that the present invention may be embodied in other specific forms without departing from the spirit or essential attributes thereof. The present embodiments are therefore to be considered in all respects as illustrative and not restrictive, the scope of the invention being indicated by the appended claims rather than by the foregoing description, and all changes which come within the meaning and range of equivalency of the claims are therefore intended to be embraced therein. Any reference sign in a claim should not be construed as limiting the claim concerned.
Claims (4)
1. A seed germination detection method in a dark scene is characterized by comprising the following steps: selecting 100 seeds, pretreating the 100 seeds to break the dormancy state, averagely dividing the 100 seeds into four groups, averagely dividing each group into five groups, wherein each group comprises 5 seeds, placing an incubator for seed germination in a dark light environment, arranging an oxygen supply device, a temperature control device, a humidity control device and a light control device, performing germination treatment on the four groups of seeds, and measuring the vitality of the seeds by a tetrazole staining method.
2. The method for detecting seed germination in dark scene according to claim 1, wherein the method comprises the following steps: low-temperature lamination pretreatment:
s1, soaking the selected 100 seed samples in clear water at the temperature of 5-10 ℃;
s2, adding the sphagna, the fine sand, the vermiculite and the peat soil into the pretreated container, and mixing the sphagna, the fine sand, the vermiculite and the peat soil in the container;
s3, burying the soaked seeds into a pretreatment container, wherein the seeds are separated by 3-5 cm;
s4, controlling the temperature inside the pretreated container to be between 5 and 10 ℃;
s5, taking the treated seeds out of the pre-treated container;
the dormancy state of the seeds is broken through a low-temperature stratification pretreatment mode.
3. The method for detecting seed germination in dark scene according to claim 1, wherein the method comprises the following steps: preparation before detection:
s1, wrapping 100 seed samples broken into dormancy states with dehydrated cotton, and absorbing moisture on the surfaces of the seeds through the dehydrated cotton;
s2, removing the skins of 100 seed samples;
s3, placing the incubator in a dark scene, and paving gauze inside the incubator;
s4, an oxygen supply device, a temperature control device, a humidity control device and a light control device are arranged at the incubator.
4. The method for detecting seed germination in dark scene according to claim 1, wherein the method comprises the following steps: and (3) detection flow:
evenly dividing 100 seeds into four groups, wherein each group is evenly divided into five groups, and each group is 5 seeds;
a first group: respectively placing 5 seeds in the five subgroups of the first large group on gauze of five incubators, keeping the oxygen content in the five incubators consistent through an oxygen supply device, keeping the humidity in the five incubators consistent through a humidity control device, keeping the light brightness in the five incubators consistent through a light control device, setting the temperature in the five incubators into five temperature areas through a temperature control device, and respectively keeping the temperature in the five incubators at 2 ℃ from low to high until the seeds germinate and then taking out;
second group: respectively placing 5 seeds in the five subgroups of the second main group on gauzes of five incubators, keeping the humidity in the five incubators consistent through a humidity control device, keeping the light brightness in the five incubators consistent through a lighting control device, keeping the temperature in the five incubators consistent through a temperature control device, setting the oxygen contents in the five incubators into five areas through an oxygen supply device, and taking out the seeds after the oxygen contents in the five incubators are respectively different by 2% from low to high until the seeds germinate;
third group: respectively placing 5 seeds in a fifth group of the third group on gauze of five incubators, keeping the oxygen content in the five incubators consistent through an oxygen supply device, keeping the temperature in the five incubators consistent through a temperature control device, keeping the light brightness in the five incubators consistent through a light control device, setting the humidity in the five incubators into five areas through a humidity control device, and respectively keeping the difference of 2% between the humidity in the five incubators from low to high until the seeds germinate and then taking out the seeds;
and a fourth group: respectively placing 5 seeds in a fifth group of five groups of the fourth group on gauze of five incubators, keeping consistent oxygen content in the five incubators through an oxygen supply device, keeping consistent humidity in the five incubators through a humidity control device, keeping consistent temperature in the five incubators through a temperature control device, setting brightness in the five incubators into five areas through a light control device, and taking out the five incubators after the seeds germinate;
the four groups of germinated seeds are respectively subjected to tetrazolium staining method to determine the viability of the seeds, the germination conditions of the seeds under the conditions of different temperatures, different humidities, different oxygen contents and different illumination are detected, and the influence degree of the conditions of different temperatures, different humidities, different oxygen contents and different illumination on the germination rate of the seeds is determined.
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| Application Number | Priority Date | Filing Date | Title |
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| CN202110632495.XA CN113207358A (en) | 2021-06-07 | 2021-06-07 | Seed germination detection method in dark scene |
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| CN202110632495.XA CN113207358A (en) | 2021-06-07 | 2021-06-07 | Seed germination detection method in dark scene |
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| CN113207358A true CN113207358A (en) | 2021-08-06 |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104686011A (en) * | 2015-03-19 | 2015-06-10 | 北京市农林科学院 | Tetrazole dyeing method for measuring eggplant seed viability |
| CN104855181A (en) * | 2015-06-17 | 2015-08-26 | 山东棉花研究中心 | Intelligent constant-temperature cotton breeding device |
| CN105009730A (en) * | 2014-04-16 | 2015-11-04 | 胡月 | Rapid method for determination of muskmelon seed vigor |
| CN106211865A (en) * | 2016-07-28 | 2016-12-14 | 甘肃省治沙研究所 | A kind of method measuring husky rice seed vigor fast and accurately |
| CN106581862A (en) * | 2016-12-16 | 2017-04-26 | 宁波城澍生物科技有限公司 | Seedling for biological guide |
| CN110734859A (en) * | 2019-11-27 | 2020-01-31 | 英都斯特(无锡)感应科技有限公司 | Magnetic field catalysis box body and application |
| CN112673855A (en) * | 2020-12-22 | 2021-04-20 | 池州市楠林园艺有限公司 | Seedling container and sowing and seedling method of tulipa edulis |
| CN213127502U (en) * | 2020-09-21 | 2021-05-07 | 刘勇 | Seed artificial containers for agricultural use |
-
2021
- 2021-06-07 CN CN202110632495.XA patent/CN113207358A/en not_active Withdrawn
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105009730A (en) * | 2014-04-16 | 2015-11-04 | 胡月 | Rapid method for determination of muskmelon seed vigor |
| CN104686011A (en) * | 2015-03-19 | 2015-06-10 | 北京市农林科学院 | Tetrazole dyeing method for measuring eggplant seed viability |
| CN104855181A (en) * | 2015-06-17 | 2015-08-26 | 山东棉花研究中心 | Intelligent constant-temperature cotton breeding device |
| CN106211865A (en) * | 2016-07-28 | 2016-12-14 | 甘肃省治沙研究所 | A kind of method measuring husky rice seed vigor fast and accurately |
| CN106581862A (en) * | 2016-12-16 | 2017-04-26 | 宁波城澍生物科技有限公司 | Seedling for biological guide |
| CN110734859A (en) * | 2019-11-27 | 2020-01-31 | 英都斯特(无锡)感应科技有限公司 | Magnetic field catalysis box body and application |
| CN213127502U (en) * | 2020-09-21 | 2021-05-07 | 刘勇 | Seed artificial containers for agricultural use |
| CN112673855A (en) * | 2020-12-22 | 2021-04-20 | 池州市楠林园艺有限公司 | Seedling container and sowing and seedling method of tulipa edulis |
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