CN106211865A - A kind of method measuring husky rice seed vigor fast and accurately - Google Patents
A kind of method measuring husky rice seed vigor fast and accurately Download PDFInfo
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- CN106211865A CN106211865A CN201610602768.5A CN201610602768A CN106211865A CN 106211865 A CN106211865 A CN 106211865A CN 201610602768 A CN201610602768 A CN 201610602768A CN 106211865 A CN106211865 A CN 106211865A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
- A01C1/02—Germinating apparatus; Determining germination capacity of seeds or the like
- A01C1/025—Testing seeds for determining their viability or germination capacity
Abstract
The present invention relates to moving dunes pionner sand rice seed seedling-raising technical field, a kind of method measuring husky rice seed vigor fast and accurately；Carry out as steps described below: seed collection and storage, Seeds preprocess, soak seed, cut seed coat, TTC solution allocation, dyeing, result statistics；Wherein cutting seed coat is that the seed filter paper of immersion is blotted surface moisture, with the seed coat at blade cutting seed germination hole, depth of cut ＜ 0.2mm, never can not injure embryo, must cut seed coat seed.The present invention only can complete with 4.5～8h；The invention provides a kind of method measuring husky rice seed vigor fast and accurately；Its most prominent feature is that cutting seed coat carries out Dormancy breaking, the vitality of husky rice seed more quickly and is accurately determined than prior art, and then before carrying out artificial culture, it is possible to accurately calculate application rate, provide precisely guarantee for husky rice in the exploration of the artificial cultivation technique in sand district, northwest.
Description
Technical field
The present invention relates to moving dunes pionner sand rice seed seedling-raising technical field, particularly one is surveyed fast and accurately
The method of Ding Sha meter seed vigor.
Background technology
Seed vigor refers to potential ability or the vitality of embryo that seed can sprout.Seed vigor is except at seed
In performance rating as a requisite measurement index outside, also the calculating of application rate in agricultural production is had guidance meaning
Justice.In agricultural production, the common method measuring seed vigor has seed germination method, TTC method, potassium iodide method etc..But,
There is a lot of problem in actual operation.Firstly, since concrete grammar selects or committed step misoperation, cause measured value
Far below actual value.Such as, if when using seed germination method to measure seed vigor. seed to be measured has dormancy, that
Measurement result is the most on the low side；In TTC method, if dyeing time and seed treatment poor timing, measured value also can
Less than actual value；Potassium iodide method is to measure the relatively effective method of coniferals seed vigor, there is also such problem；Its
Secondary, a lot of methods all provide a scope in the step of some concrete key.Such as, the time 3～8h soaked seed and dye, right
May be different in concrete species；This, survey with reference to TTC method or potassium iodide method seed vigor if some species is strict again
Fixed, the vitality of mensuration is likely to well below emergence rate.It addition, particularly in desert ecological system, seed vigor
Measure the method usually with reference to agriculture field, but, owing in desert ecological system, environmental difference is big, so, the seed of generation
Form and structure differ greatly with agricultural seed, therefore, in the mensuration of psammophytes seed vigor, can only be with reference to agriculture
The method in industry field, for concrete species, the aspects such as assay method selects, committed step all should be made according to seed feature
Corresponding change, and then explore a set of effective method.
Sha Peng, be commonly called as husky rice (Agriophyllum squarrosum), it is under the jurisdiction of the husky fluffy genus one of Chenopodiaceae
Year raw plant, is distributed widely in northern China Ge Sha district, for moving dunes pionner；It not only has good wind prevention and sand resistance and makees
With, and seed nutritional enriches, the food that Shi Sha district people like since ancient times.Research in recent years finds, husky rice is a kind of natural
Functional food, is particularly suitable for child and designed for old people.
Along with husky rice food understanding is improved constantly by people, husky rice food demand constantly increases, only by wild husky rice
The seed gathered can not meet far away the demand of people.Therefore, people start to explore its artificial cultivation technique.Carrying out Planting
Before training, the mensuration of husky rice seed vigor is extremely important for accurately calculating application rate.But, due to husky district environmental difference
Greatly, husky meter Zi Shen plasticity is strong；So, the seed variability in different husky districts is big, including seed quality, size, water absorption rate, sprouting spy
Property etc..There is not dormancy in the seed that some husky district gathers, some then exists dormancy；So, find a set of energy and be suitable for
Assay method in different husky districts sand rice seed vigor has actual application value.
Wang Yuqing etc. explore husky rice seed vigor assay method.The key technology of the method punctures seed coat and enhances kind
The water absorption of son, shortens the time of dyeing, has broken the dormancy of seed.Applicant is punctured by substantial amounts of husky rice seed and is cut
Cut seed coat carry out Dormancy breaking contrast find: puncture kind of pico farad from Seeds preprocess to germination rate more than 90 need 14d time
Between, and cut kind of a pico farad breaking dormancy and have only to 3～4d, germination rate reaches the 99.5～100%(seeds floating on the water surface also life
Vigor).Additionally, cutting seed coat method is also applicable for there is not the mensuration of the husky rice seed vigor of dormancy；Such as: adopt
Seed germination rate in Dunhuang reaches more than 98% and needs 17d, adopts the seed germination rate in Shapotou and reaches more than 98% and need
20d.And they reach this germination rate and only need 4d to use cutting seed coat method.So, cutting seed coat compared with acupuncture seed coat, energy
Seed water absorption is enough greatly enhanced, shortens dyeing time and quickly break seed dormancy.Applicant according to this achievement in research,
In conjunction with TTC method, by repeatedly verifying, condensed go out a set of to survey than prior art more husky rice seed vigor
Fixed new method, provides the guarantee of accurately calculating application rate for exploring of the husky rice artificial cultivation technique in sand district, northwest.
Summary of the invention
It is an object of the invention to provide one husky rice seed vigor assay method fast and accurately；The method is to existing
Husky rice seed can carry out quickly and accurately viability test, and then accurately calculates application rate.
In order to realize described purpose, the technical scheme that the present invention takes is as follows: one measures husky rice seed fast and accurately
Blodynamic method, is carried out as steps described below: seed collection and storage, Seeds preprocess, soaks seed, cut seed coat, TTC solution
Configuration, dyeing, result statistics；Concrete steps include:
1. seed collection and storage
1.1 seed collections: annual by the end of September to mid or late October growth selection on the moving dunes of husky rice distributing region
Healthy and strong plant, the results seed when major part seed maturity, the sex character of seed maturity is that plant soon dries up, seed therein
In faint yellow (with Semen Tritici aestivi color similarity)；With shears, fruit ear is cut, be exposed to the sun under the sun, with shaking off and beating the side ground after drying
Formula obtains seed, then removes the foreign material such as branch, blade；
1.2 seed room storage: the seed removing foreign material results is loaded in muslin bag and at room temperature stores, avoid during storage
Sootiness, the plague of rats, insect pest, water are wet etc.；Storage room temperature 13～30 DEG C, relative humidity ＜ 30%；
2. Seeds preprocess
2.1 seed preparations: taken out from muslin bag by seed, random number takes 400, seed；
2.2 seed disinfections: seed is inserted the HgCl of mass percent concentration 0.1%2Reagent soaks 6～10min taking-ups, uses
Distilled water rinses 3～5 times repeatedly；Obtain disinfection seed；
3. seed soaking: be soaked in water under 30～35 DEG C of dark conditions 2h by disinfection seed, and during immersion, every 30min changes water once；
4. cutting seed coat
The seed filter paper of immersion is blotted surface moisture, and (seed is recessed then to cut the seed coat at seed germination hole with blade
One gone is germinal aperature), depth of cut ＜ 0.2mm, never can not injure embryo, husky rice seed is annular embryo, and annular embryo divides
The surrounding of cloth seed, must cut seed coat seed；
5. configuration TTC staining solution
Weigh 1.0 g TTC and put in small beaker, after first adding the dissolving of a little ethanol, then be settled to 100 with distilled water diluting
ML, is configured to the TTC solution that mass percent concentration is 1.0%；TTC solution, with joining, should not be hidden for a long time；Solution should hide
Light is preserved.If having turned into redness, then can not be continuing with.
6. dyeing
Cutting seed coat seed is put into the container containing 1.0% TTC solution seed is dyeed, with submergence seed for degree, be placed in perseverance
Incubator, is incubated 2～6h in 30 DEG C～35 DEG C, obtains dyeing seed；
7. result statistics
Dyeing seed is taken out, repeatedly rinses with distilled water 3～5 times, then wipe the moisture on dry surface with filter paper；First to germination
Seed add up；Then the seed not germinateed is dissected, if embryo dyes redness, it is believed that seed is vibrant, if embryo is not
Dye redness, it is believed that seed debility；It is great-hearted seed number that chitting piece number and embryo dye red-coloured seeds number sum, finally
Calculating seed vigor:
Seed vigor=(chitting piece number+embryo dyes red-coloured seeds number)/sample seed sum × 100%.
Optimal prior art measures husky rice seed vigor needs 26～30h, and the present invention only can complete with 4.5～8h；
The invention provides a kind of method measuring husky rice seed vigor fast and accurately；Its most prominent feature is that cutting seed coat is big
Amplitude enhances seed water absorption, shortens the time of dyeing and quick Dormancy breaking.Quicker and accurate than prior art
The vitality determining husky rice seed, and then before carrying out artificial culture, it is possible to accurately calculate application rate, for husky rice in west
The exploration of the artificial cultivation technique in Bei Sha district provides precisely guarantee.
Detailed description of the invention
Embodiment 1
1. seed collection and storage
1.1 seed collections: on October 25th, 2013 moving dunes at Minqin, Gansu Province comprehensive sand control experimental station (38 ° of 34 ' N,
102 ° of 58 ' E) go up plant 20 strain that growth selection is healthy and strong, with shears, fruit ear is cut, under the sun when major part seed maturity
It is exposed to the sun, shakes off after drying and beat the mode ground to obtain seed, then remove the foreign material such as branch, blade.
1.2 seed room storage: results seed about the 200g removing foreign material is loaded muslin bag on November 2nd, 2013
In at room temperature store, avoid sootiness, the plague of rats, insect pest, water wet during storage etc.；Storage room temperature 13～30 DEG C, relative humidity ＜
30%。
2. Seeds preprocess
2.1 seed preparations: seed to be measured is taken out from muslin bag by April 26th, 2015, and random number takes 400, seed；
2.2 seed disinfections: 400 seeds are inserted the HgCl of mass percent concentration 0.1%2Reagent soaks 10min take out,
Repeatedly rinse with distilled water 5 times；Obtain disinfection seed.
3. seed soaking: by disinfection seed in dark conditions with 35 DEG C of water soaking 2h, often 30min changes water one in the process
Secondary, change water altogether 3 times.
4. cutting seed coat
400 the seed filter paper soaked are blotted surface moisture by the same day on April 26th, 2015, then sprout with blade cutting seed
Seed coat at hair engaging aperture (seed fall in one be germinal aperature), depth of cut ＜ 0.2mm, never can not injure embryo, husky rice
Seed is annular embryo, the surrounding of annular embryo distribution seed, must cut seed coat seed.
5. configuration TTC solution
The same day on April 26th, 2015 configures 1.0% TTC solution 100mL；Specific practice: weigh 1.0 g TTC and put into little burning
In Bei, after first adding the dissolving of a little ethanol, then being settled to 100 mL with distilled water diluting, being configured to mass percent concentration is
The TTC solution of 1.0%.
6. dyeing
On the same day on April 26th, 2015,400 cutting seed coat seeds are put into the container containing 1.0% TTC solution, with submergence
Seed is degree, is placed in the calorstat of 35 DEG C insulation 4h, obtains dyeing seed.
7 result statistics
On the same day on April 26th, 2015, dyeing seed is taken out, with distilled water flushing 5 times, then wipes the water on dry surface with filter paper
Point, statistics shows: the seed number of germination 304, does not germinates and dyes the seed number 95 of red embryo, does not germinates and also do not dye redness
The seed number of embryo 1；The vitality 99.75% of seed.
Embodiment 2
1. seed collection and storage
1.1 seed collections: on the October 20th, 2013 of the moving dunes at In Shapotou of Ningkia experimental station (37 ° of 27 ' N, 104 ° of 57 ' E)
Plant 25 strain that upper growth selection is healthy and strong, remaining processes with embodiment 1.
1.2 seed room storage: results seed about the 300g removing foreign material is loaded cotton on October 29th, 2013
At room temperature storing in Dai, remaining is with embodiment 1.
2. Seeds preprocess
2.1 seed preparations: seed to be measured is taken out from muslin bag by April 20th, 2014, and random number takes 400, seed；
2.2 seed disinfections: 400 seeds are inserted the HgCl of mass percent concentration 0.1%2Reagent soaks 8min take out, use
Distilled water rinses 4 times repeatedly；Obtain disinfection seed.
3. seed soaking: by disinfection seed in dark conditions with 30 DEG C of water soaking 2h, remaining is with embodiment 1.
4. cutting seed coat
400 the seed filter paper soaked are blotted surface moisture by the same day on April 20th, 2014, and remaining operation is with embodiment 1.
5. configuration TTC solution
The same day on April 20th, 2015 configures 1.0% TTC solution 100mL；Specific practice: with embodiment 1.
6. dyeing
On the same day on April 20th, 2015,400 cutting seed coat seeds are put into the container containing 1.0% TTC solution, with submergence
Seed is degree, is placed in the calorstat of 30 DEG C insulation 6h, obtains dyeing seed.
7 result statistics
On the same day on April 20th, 2015, dyeing seed is taken out, with distilled water flushing 4 times, then wipes the water on dry surface with filter paper
Point, statistics shows: the seed number of germination 384, does not germinates and dyes the seed number 16 of red embryo, the vitality 100% of seed.
Embodiment 3
1. seed collection and storage
1.1 seed collections: on the October 20th, 2012 of the moving dunes in Dunhuang (39 ° of 53 ' N, 92 ° of 13 ' E), upper growth selection was good for
Strong plant 30 strain, remaining processes with embodiment 1.
1.2 seed room storage: results seed about the 200g removing foreign material is loaded muslin bag on November 1st, 2012
In at room temperature store, remaining is with embodiment 1.
2. Seeds preprocess
2.1 seed preparations: seed to be measured is taken out from muslin bag by May 20th, 2013, and random number takes 400, seed；
2.2 seed disinfections: 400 seeds are inserted the HgCl of mass percent concentration 0.1%2Reagent soaks 6min take out, use
Distilled water rinses 4 times repeatedly；Obtain disinfection seed；
3. seed soaking: by disinfection seed in dark conditions with 32 DEG C of water soaking 2h, remaining is with embodiment 1.
4. cutting seed coat
On the same day on May 20th, 2014,400 the seed filter paper soaked being blotted surface moisture, remaining operation is with embodiment 1.
The configuration of 5.TTC solution
On the same day on May 20th, 2014, configure 1.0% TTC solution 100mL；Specific practice: with embodiment 1.
6. dyeing
On the same day on May 20th, 2014,400 cutting seed coat seeds are put into the container containing 1.0% TTC solution, with submergence
Seed is degree, is placed in the calorstat of 33 DEG C insulation 5h, obtains dyeing seed.
7. result statistics
On the same day on May 20th, 2014, dyeing seed is taken out, with distilled water flushing 3 times, then wipes the water on dry surface with filter paper
Point, statistics shows: the seed number of germination 316, does not germinates and dyes the seed number 84 of red embryo, the vitality 100% of seed.
Embodiment 4
1. seed collection and storage
1.1 seed collections: on October 22nd, 2014 moving dunes at Minqin, Gansu Province comprehensive sand control experimental station (38 ° of 34 ' N,
102 ° of 58 ' E) go up plant 30 strain that growth selection is healthy and strong, remaining operation is with embodiment 1.
1.2 seed room storage: results seed about the 250g removing foreign material is loaded muslin bag on November 3rd, 2014
In at room temperature store, remaining is with embodiment 1.
2. Seeds preprocess
2.1 seed preparations: seed to be measured is taken out from muslin bag by May 18th, 2015, and random number takes 400, seed；
2.2 seed disinfections: 400 seeds are inserted the HgCl of mass percent concentration 0.1% by the same day on May 18th, 20152Reagent
Middle immersion 9min takes out, and repeatedly rinses with distilled water 3 times；Obtain disinfection seed.
3 seed soaking: by disinfection seed in dark conditions with 33 DEG C of water soaking 2h, remaining is with embodiment 1.
4. cutting seed coat
Seed filter paper in 2.3 is blotted surface moisture by the same day on May 18th, 2015, and remaining is with embodiment 2；
5. configuration TTC solution
The same day on May 18th, 2015 configures 1.0% TTC solution 100mL；Specific practice is with embodiment 1
6. dyeing
On the same day on May 18th, 2015,400 cutting seed coat seeds are put into the container containing 1.0% TTC solution, with submergence
Seed is degree, is placed in the calorstat of 35 DEG C insulation 2h, obtains dyeing seed.
7 result statistics
On the same day on May 18th, 2015, dyeing seed is taken out, with distilled water flushing 4 times, then wipes the water on dry surface with filter paper
Point, statistics shows: the seed number of germination 52, does not germinates and dyes the seed number 346 of red embryo, does not germinates and also do not dye redness
The seed number of embryo 2；The vitality 99.5% of seed.
Claims (1)
1. the method measuring husky rice seed vigor fast and accurately, it is characterised in that carry out as steps described below: seed
Gather with storage, Seeds preprocess, soak seed, cut seed coat, TTC solution allocation, dyeing, result add up；Concrete steps include:
(1). seed collection and storage
1.1 seed collections: annual by the end of September to mid or late October growth selection on the moving dunes of husky rice distributing region
Healthy and strong plant, the results seed when major part seed maturity, the sex character of seed maturity is that plant soon dries up, seed therein
In faint yellow (with Semen Tritici aestivi color similarity)；With shears, fruit ear is cut, be exposed to the sun under the sun, with shaking off and beating the side ground after drying
Formula obtains seed, then removes the foreign material such as branch, blade；
1.2 seed room storage: the seed removing foreign material results is loaded in muslin bag and at room temperature stores, avoid during storage
Sootiness, the plague of rats, insect pest, water are wet etc.；Storage room temperature 13～30 DEG C, relative humidity ＜ 30%；
(2). Seeds preprocess
2.1 seed preparations: taken out from muslin bag by seed, random number takes 400, seed；
2.2 seed disinfections: seed is inserted the HgCl of mass percent concentration 0.1%2Reagent soaks 6～10min taking-ups, with steaming
Distilled water rinses 3～5 times repeatedly；Obtain disinfection seed；
(3). seed soaking: be soaked in water under 30～35 DEG C of dark conditions 2h by disinfection seed, and during immersion, every 30min changes water one
Secondary；
(4). cutting seed coat
The seed filter paper of immersion is blotted surface moisture, and (seed is recessed then to cut the seed coat at seed germination hole with blade
One gone is germinal aperature), depth of cut ＜ 0.2mm, never can not injure embryo, husky rice seed is annular embryo, and annular embryo divides
The surrounding of cloth seed, must cut seed coat seed；
(5). configuration TTC staining solution
Weigh 1.0 g TTC and put in small beaker, after first adding the dissolving of a little ethanol, then be settled to 100 with distilled water diluting
ML, is configured to the TTC solution that mass percent concentration is 1.0%；
(6). dyeing
Cutting seed coat seed is put into the container containing 1.0% TTC solution seed is dyeed, with submergence seed for degree, be placed in perseverance
Incubator, is incubated 2～6h in 30 DEG C～35 DEG C, obtains dyeing seed；
(7). result is added up
Dyeing seed is taken out, repeatedly rinses with distilled water 3～5 times, then wipe the moisture on dry surface with filter paper；First to germination
Seed add up；Then the seed not germinateed is dissected, if embryo dyes redness, it is believed that seed is vibrant, if embryo is not
Dye redness, it is believed that seed debility；It is great-hearted seed number that chitting piece number and embryo dye red-coloured seeds number sum, finally
Calculating seed vigor:
Seed vigor=(chitting piece number+embryo dyes red-coloured seeds number)/sample seed sum × 100%.
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN110226381A (en) * | 2018-11-08 | 2019-09-13 | 河西学院 | One seed sand rice seed dormancy restructuring bankrupt |
| CN111328497A (en) * | 2020-03-09 | 2020-06-26 | 浙江省农业科学院 | Method for measuring activity of crop seeds |
| CN112930760A (en) * | 2021-04-05 | 2021-06-11 | 兰州大学 | Tetrazole determination method for sweet pepper seed viability |
| CN113207358A (en) * | 2021-06-07 | 2021-08-06 | 武汉沃图农擎科技有限公司 | Seed germination detection method in dark scene |
| CN115530023A (en) * | 2022-11-09 | 2022-12-30 | 中国科学院新疆生态与地理研究所 | Method for rapidly supplementing degenerated ecosystem seed source in arid region through artificial seed floating |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110226381A (en) * | 2018-11-08 | 2019-09-13 | 河西学院 | One seed sand rice seed dormancy restructuring bankrupt |
| CN111328497A (en) * | 2020-03-09 | 2020-06-26 | 浙江省农业科学院 | Method for measuring activity of crop seeds |
| CN111328497B (en) * | 2020-03-09 | 2021-12-17 | 浙江省农业科学院 | Method for measuring activity of crop seeds |
| CN112930760A (en) * | 2021-04-05 | 2021-06-11 | 兰州大学 | Tetrazole determination method for sweet pepper seed viability |
| CN113207358A (en) * | 2021-06-07 | 2021-08-06 | 武汉沃图农擎科技有限公司 | Seed germination detection method in dark scene |
| CN115530023A (en) * | 2022-11-09 | 2022-12-30 | 中国科学院新疆生态与地理研究所 | Method for rapidly supplementing degenerated ecosystem seed source in arid region through artificial seed floating |
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