CN113200953A - Method for extracting anthocyanin - Google Patents

Method for extracting anthocyanin Download PDF

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Publication number
CN113200953A
CN113200953A CN202110520457.5A CN202110520457A CN113200953A CN 113200953 A CN113200953 A CN 113200953A CN 202110520457 A CN202110520457 A CN 202110520457A CN 113200953 A CN113200953 A CN 113200953A
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CN
China
Prior art keywords
anthocyanin
extracting
cell wall
wall breaking
anthocyanidin
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN202110520457.5A
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Chinese (zh)
Inventor
谢奇奇
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Xiangyang Inas Biotechnology Co ltd
Original Assignee
Xiangyang Inas Biotechnology Co ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
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Priority to CN202110520457.5A priority Critical patent/CN113200953A/en
Publication of CN113200953A publication Critical patent/CN113200953A/en
Pending legal-status Critical Current

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    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07DHETEROCYCLIC COMPOUNDS
    • C07D311/00Heterocyclic compounds containing six-membered rings having one oxygen atom as the only hetero atom, condensed with other rings
    • C07D311/02Heterocyclic compounds containing six-membered rings having one oxygen atom as the only hetero atom, condensed with other rings ortho- or peri-condensed with carbocyclic rings or ring systems
    • C07D311/04Benzo[b]pyrans, not hydrogenated in the carbocyclic ring
    • C07D311/58Benzo[b]pyrans, not hydrogenated in the carbocyclic ring other than with oxygen or sulphur atoms in position 2 or 4
    • C07D311/60Benzo[b]pyrans, not hydrogenated in the carbocyclic ring other than with oxygen or sulphur atoms in position 2 or 4 with aryl radicals attached in position 2
    • C07D311/62Benzo[b]pyrans, not hydrogenated in the carbocyclic ring other than with oxygen or sulphur atoms in position 2 or 4 with aryl radicals attached in position 2 with oxygen atoms directly attached in position 3, e.g. anthocyanidins
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L2/00Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
    • A23L2/42Preservation of non-alcoholic beverages
    • A23L2/44Preservation of non-alcoholic beverages by adding preservatives
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L2/00Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
    • A23L2/52Adding ingredients
    • A23L2/58Colouring agents
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L3/00Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs
    • A23L3/34Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs by treatment with chemicals
    • A23L3/3454Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs by treatment with chemicals in the form of liquids or solids
    • A23L3/3463Organic compounds; Microorganisms; Enzymes
    • A23L3/3544Organic compounds containing hetero rings
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/105Plant extracts, their artificial duplicates or their derivatives
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L5/00Preparation or treatment of foods or foodstuffs, in general; Food or foodstuffs obtained thereby; Materials therefor
    • A23L5/40Colouring or decolouring of foods
    • A23L5/42Addition of dyes or pigments, e.g. in combination with optical brighteners
    • A23L5/43Addition of dyes or pigments, e.g. in combination with optical brighteners using naturally occurring organic dyes or pigments, their artificial duplicates or their derivatives
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2002/00Food compositions, function of food ingredients or processes for food or foodstuffs
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/90Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in food processing or handling, e.g. food conservation

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Polymers & Plastics (AREA)
  • Health & Medical Sciences (AREA)
  • Nutrition Science (AREA)
  • Engineering & Computer Science (AREA)
  • Food Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Microbiology (AREA)
  • Botany (AREA)
  • Mycology (AREA)
  • Coloring Foods And Improving Nutritive Qualities (AREA)

Abstract

The invention discloses a method for extracting anthocyanin, which comprises the steps of crushing selected raw materials, breaking cell walls of the crushed raw materials through a cell wall breaking technology, extracting, filtering, performing preservative treatment and performing vacuum freeze drying to obtain the high-purity anthocyanin convenient for human body absorption. The invention relates to a method for extracting anthocyanin, wherein the cell wall is firstly crushed by a cell wall breaking technology in the process of extracting the anthocyanin, the cell wall breaking technology can fully release effective substances, the nutrient content and the efficacy of food are improved by at least 10 times, the nutrition is better released, and the absorption by a human body is facilitated. The extraction process is simple and easy to operate, and the anthocyanin is high in extraction yield and purity and suitable for popularization and application.

Description

Method for extracting anthocyanin
Technical Field
The invention relates to the field of anthocyanin extraction, and particularly relates to a method for extracting anthocyanin.
Background
Flavones (flavanols) and their glycosides (flavanoids) are also called vitamin P and anthocyanins, which are one of the essential nutrients for human body. It is a natural pigment. More than 500 have been found so far. The flavonoids are widely distributed in the plant, and more than 20 percent of the botanical drugs contain the flavonoids. The flavone compounds have effects in treating hypertension, edema, connective tissue disease, allergy, asthma, cancer, cardiovascular disease, gastrointestinal ulcer pain, senile dementia, stimulating injured recovered immune system, preventing acquired immunodeficiency syndrome (such as AIDS), contraception, etc., and has plant hormone behavior, and research shows that the extract with certain anthocyanin concentration can effectively prevent carcinogenesis of different stages. However, the individual action of anthocyanins is not determined, in part because anthocyanins are easily degraded when they are separated from other stable components such as phenols and the like and then subjected to bioassay. With the development of science and technology, people pay more and more attention to the safety of food additives, the development and utilization of natural additives become the general trend of additive development and use, anthocyanin can be used as a nutrition enhancer in food, can also be used as a food preservative to replace synthetic preservatives such as benzoic acid and the like, can be used as a food colorant to be applied to common beverages and food, and meets the general requirements of people on the natural, safe and healthy of food additives.
Disclosure of Invention
The invention aims to provide a method for extracting anthocyanin.
The invention provides the following technical scheme:
a method for extracting anthocyanidin comprises crushing selected raw materials, breaking cell walls of the crushed raw materials by a cell wall breaking technology, extracting, filtering, performing antiseptic treatment and vacuum freeze drying to obtain high-purity anthocyanidin which is convenient for human body absorption.
Further, the cell wall breaking technology breaks the cell wall through a cell wall breaking machine, and the broken wall is pulverized into 1000-2500-mesh ultrafine powder with the diameter of about 10 μm.
Further, the anthocyanin is extracted by soaking the crushed raw materials in ethanol for 1-2 hours.
Further, the solid obtained by filtering is dried, and the dried solid is put into a warehouse for storage.
Further, citric acid is added to the filtered anthocyanin solution, and then the anthocyanin is pasteurized.
Further freezing the anthocyanin after the preservative treatment in a temperature range of-20 ℃ to-50 ℃, and freeze-drying the frozen product in a freeze dryer under the condition of vacuumizing and freeze-drying for 1h at-35 ℃, then raising the temperature to-20 ℃, keeping the temperature for 3h, then gradually raising the temperature to 20 ℃, and finally obtaining the finished anthocyanin after drying.
Compared with the prior art, the invention has the following beneficial effects:
the invention relates to a method for extracting anthocyanin, wherein the cell wall is firstly crushed by a cell wall breaking technology in the process of extracting the anthocyanin, the cell wall breaking technology can fully release effective substances, the nutrient content and the efficacy of food are improved by at least 10 times, the nutrition is better released, and the absorption by a human body is facilitated. The extraction process is simple and easy to operate, and the anthocyanin is high in extraction yield and purity and suitable for popularization and application.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below in conjunction with the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all embodiments, and all other embodiments obtained by a person of ordinary skill in the art without creative efforts based on the embodiments of the present invention belong to the protection scope of the present invention.
The method for extracting the anthocyanin comprises the steps of crushing the selected raw materials, breaking cell walls of the crushed raw materials through a cell wall breaking technology, extracting, filtering, performing preservative treatment and performing vacuum freeze drying to obtain the high-purity anthocyanin convenient for human body absorption.
Through the scheme of the invention, the cell wall breaking technology breaks the cell wall through a cell wall breaking machine, and the broken wall is crushed into about 1000-2500-mesh ultrafine powder with the diameter of about 10 microns.
According to the scheme, the anthocyanin is extracted by soaking the crushed raw materials in ethanol for 1-2 hours.
Through the scheme of the invention, the solid and the solution can be filtered out through filtration, and the filtered solution is the anthocyanin solution.
According to the scheme of the invention, the solid obtained by filtering is dried, and the dried solid is put into a warehouse for storage.
Through the scheme, citric acid is added into the filtered high-purity anthocyanin, and then the anthocyanin is pasteurized.
Through the scheme, the anthocyanin after preservative treatment is frozen in a temperature range of-20 ℃ to-50 ℃, the frozen product is freeze-dried in a freeze dryer under the condition that the freeze-drying is carried out at-35 ℃ for 1h, then the temperature is raised to-20 ℃, the temperature is kept for 3h, then the temperature is gradually raised to 20 ℃, and the finished product anthocyanin is obtained after the drying is finished.
In the specific application, the invention relates to a method for extracting anthocyanin, wherein the cell wall is firstly crushed by a cell wall breaking technology in the process of extracting the anthocyanin, the cell wall breaking technology can fully release effective substances, the nutrient content and the efficacy of food are improved by at least 10 times, the nutrition is better released, and the absorption by a human body is facilitated. The extraction process is simple and easy to operate, and the anthocyanin is high in extraction yield and purity and suitable for popularization and application.
Finally, it should be noted that: although the present invention has been described in detail with reference to the foregoing embodiments, those skilled in the art will understand that various changes, modifications and substitutions can be made without departing from the spirit and scope of the invention as defined by the appended claims. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.

Claims (6)

1. The method for extracting the anthocyanin is characterized by comprising the steps of crushing the selected raw materials, breaking cell walls of the crushed raw materials through a cell wall breaking technology, extracting, filtering, performing preservative treatment and performing vacuum freeze drying to obtain the high-purity anthocyanin convenient for human body absorption.
2. The method for extracting anthocyanidin according to claim 1, wherein the cell wall breaking technology breaks cell walls through a cell wall breaking machine, and the cell walls are broken and pulverized into 1000-2500-mesh ultrafine powder.
3. The method for extracting anthocyanidin according to claim 1, wherein the extraction is carried out by soaking the crushed raw material in ethanol for 1-2 hours.
4. The method for extracting anthocyanidin according to claim 1, wherein the solid obtained by filtration is dried and the dried solid is stored in a warehouse.
5. The method for extracting anthocyanidin according to claim 1, wherein citric acid is added to the filtered anthocyanidin solution, and then the anthocyanidin is pasteurized.
6. The method for extracting anthocyanin according to claim 1, wherein the anthocyanin after preservative treatment is frozen at the temperature ranging from-20 ℃ to-50 ℃, the frozen product is freeze-dried in a freeze dryer under the conditions of-35 ℃ vacuum freeze-drying for 1h, then the temperature is raised to-20 ℃, the temperature is kept constant for 3h, then the temperature is gradually raised to 20 ℃, and the finished anthocyanin is obtained after drying is finished.
CN202110520457.5A 2021-05-13 2021-05-13 Method for extracting anthocyanin Pending CN113200953A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN202110520457.5A CN113200953A (en) 2021-05-13 2021-05-13 Method for extracting anthocyanin

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN202110520457.5A CN113200953A (en) 2021-05-13 2021-05-13 Method for extracting anthocyanin

Publications (1)

Publication Number Publication Date
CN113200953A true CN113200953A (en) 2021-08-03

Family

ID=77031019

Family Applications (1)

Application Number Title Priority Date Filing Date
CN202110520457.5A Pending CN113200953A (en) 2021-05-13 2021-05-13 Method for extracting anthocyanin

Country Status (1)

Country Link
CN (1) CN113200953A (en)

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103113341A (en) * 2013-02-01 2013-05-22 江苏万村爱农农业科技有限公司 Process for extracting blueberry anthocyanin
CN105884737A (en) * 2015-01-25 2016-08-24 东北林业大学 Purification technology of sweetberry honeysuckle anthocyanin
CN108530412A (en) * 2018-06-07 2018-09-14 盛林蓝莓集团股份有限公司 A kind of extracting method of blueberry anthocyanin
CN110128391A (en) * 2019-06-14 2019-08-16 西安科技大学 Method for extracting cationic anthocyanin from lonicera edulis
CN111961023A (en) * 2020-08-17 2020-11-20 上海培勇生物科技有限公司 Method for extracting anthocyanin

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103113341A (en) * 2013-02-01 2013-05-22 江苏万村爱农农业科技有限公司 Process for extracting blueberry anthocyanin
CN105884737A (en) * 2015-01-25 2016-08-24 东北林业大学 Purification technology of sweetberry honeysuckle anthocyanin
CN108530412A (en) * 2018-06-07 2018-09-14 盛林蓝莓集团股份有限公司 A kind of extracting method of blueberry anthocyanin
CN110128391A (en) * 2019-06-14 2019-08-16 西安科技大学 Method for extracting cationic anthocyanin from lonicera edulis
CN111961023A (en) * 2020-08-17 2020-11-20 上海培勇生物科技有限公司 Method for extracting anthocyanin

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Application publication date: 20210803