KR100511802B1 - Extraction Method of Resveratrol from Grape - Google Patents

Abstract

The present invention relates to a method for extracting resveratrol by using a water-ethanol mixed solvent and ultrasonic wave as a raw material of grapes as a raw material, which is sun-dried for 1 month using a pine branch of grapes as a raw material, and has a particle size of 0.3 to 0.5 mm The pulverized pulverized product as \ was used as an extraction material, and 5-10 L of solvent was added per kg of the dried pulverized product, using a mixture of water and ethanol at a volume basis of 2: 8 to 3: 7 as a solvent. Subsequently, the ultrasonic wave of 45-50 kHz at room temperature is treated for 3 to 5 minutes at an energy intensity of 0.03 to 0.05 kW per 1 L of the pulverized powder, thereby extracting resveratrol, which is safe and has an excellent extraction effect.

Description

Extraction Method of Resveratrol from Grape

The present invention relates to a method for extracting resveratrol from grapes, and more particularly, to a method for extracting resveratrol by using water / ethanol mixed solvent and sonication, using a cluster of grape branches as a raw material.

Resveratrol (3,4'5-trihydroxystilbene) is a phytoalexin compound found in grapes and various plants and has various physiological and pharmacological effects including anticancer, antithrombotic, and anti-inflammatory activity. In particular, resveratrol has recently emerged as a phytoestrogen compound having a female hormone estrogen-like action, and has recently been in the spotlight as a bioactive substance that suppresses cardiac disease, osteoporosis, dementia and various menopausal symptoms after menopause.

Conventionally, in order to obtain such resveratrol, a method of extracting resveratrol by heating an organic solvent such as ethanol, methanol, acetone, etc., using grape vines or grape fruit skin and seeds as raw materials has been used.

In the extraction by such a conventional organic solvent, the extraction conditions for 20 hours at 30 ° C or 30 minutes at 60 ° C have become the maximum extraction conditions. Extraction conditions in the conventional extraction method has a drawback that takes a lot of extraction time in the low and medium temperature conditions, extraction time is shortened in high temperature conditions, but there is a high risk of explosion of the organic solvent. On the other hand, if the extraction conditions are too strong or the extraction time is longer than necessary, there is a fear that the extracted resveratrol is rather decomposed and lost.

Therefore, in the method of extracting resveratrol from grapes using the conventional organic solvent as described above, there is an extraction scheme that can solve the operational risks and product decomposition risks at high temperature extraction, process efficiency degradation due to long time operation at low temperature extraction The need has been raised.

The present invention has been made to solve the above problems, it is to provide a resveratrol extraction method that can be carried out under safe process conditions while high extraction efficiency of resveratrol by extracting grapes using an organic solvent and ultrasonic waves.

In extracting resveratrol from grapes, the present invention

a) drying the grape cluster;

b) grinding the grape cluster branches to obtain a pulverized product;

c) introducing the milled product into a solvent;

d) ultrasonically treating the solvent into which the pulverized powder is added;

It consists of a method comprising a.

The grape may be used as a normal grape, it is preferable to use a cluster branch site with a high content of resveratrol.

The drying may be forced drying using a dryer and natural drying, but for natural preservation of the ingredients contained in the raw material is preferably natural drying, more preferably daylight drying.

The pulverization of the grape raw material may use a grinder commonly used in the field of the present invention, there is no particular limitation. The pulverized product preferably has a particle size of 0.3-0.5 mm, which is intended to increase efficiency in extraction by extraction solvent and ultrasonic waves. If it exceeds 0.5 mm, the extraction efficiency of the solvent becomes low, and if it is too small, workability becomes bad.

In a preferred embodiment of the invention, the solvent may be water, ethanol, methanol, acetone and the like, it is preferable to use a mixture of water and ethanol, methanol, acetone, etc., it is most preferable to use a mixture of water and ethanol desirable. In a preferred embodiment of the invention, 5 to 10 L of a solvent mixed with water and ethanol at a volume basis of 2: 8 to 3: 7 is added per kg of the pulverized product.

It is preferable to increase the extraction effect by treating the solvent in which the pulverized product is added with ultrasonic waves. If it is not treated with ultrasonic waves, the extraction rate is slow at low temperatures, and there is a problem in that it is heated to a high temperature in order to increase the extraction rate.

Ultrasound is more preferably treated for 3 to 5 minutes at room temperature with an energy intensity of 0.03 to 0.05 kW per 1 L solution in the range of 45 to 50 kHz for efficient extraction of resveratrol.

Reference may be made to the following examples for easier implementation of the invention, and it will be apparent to those skilled in the art that the following examples are not to be construed as limiting the scope of the invention.

EXAMPLE

Example 1

Pine branches were isolated from the purchased grape grapes and sun-dried for 1 month. The dried clustered branches were crushed to an average diameter of 0.4 mm using a grinder, and then 150 g of the ground product was added to a solution in which 250 ml of water and 750 ml of ethanol were mixed. The ultrasonic generator was used to generate ultrasonic waves at a frequency of 50 kHz at room temperature and treated for 3 minutes.

The weight of the obtained resveratrol was measured as a result of comparing with the weight of the dry matter.

Example 2

It carried out for 5 minutes by ultrasonic waves under the same conditions as in Example 1.

Example 3

It was carried out under the same conditions as in Example 1 using Campbell grapes.

Example 4.

It was carried out under the same conditions as in Example 2 using Campbell grapes.

Comparative Example 1

Pine branches were isolated from the purchased grape grapes and sun-dried for 1 month. The dried clustered branches were pulverized to an average diameter of 0.4 mm using a grinder, and then 150 g of the ground product was added to a 60 ° C. solution in which 250 ml of water and 750 ml of ethanol were mixed. The extraction was terminated after 30 minutes and the weight of the resveratrol obtained was compared with the weight of the dry matter.

Comparative Example 2

Campbell grapes were used under the same conditions as in Comparative Example 1.

Table 1 below shows the results of comparing the extraction amount of resveratrol extracted by using the cluster of grapes as a raw material of the present invention with the amount extracted by the conventional method.

No. Weight of Resveratrol Extracted from Building Weight of Raw Material (%) Example 1       0.0316 Example 2       0.0332 Example 3       0.0876 Example 4       0.0826 Comparative Example 1       0.0261 Comparative Example 2       0.0706

In extracting resveratrol from grapes by the method of the present invention, a pine branch having a relatively high content of resveratrol is used as a raw material, and extracted using an organic solvent and ultrasonic wave to extract resveratrol at a safe and safe process condition. It was to provide a resveratrol extraction method that can be carried out and the resveratrol extracted thereby.

Claims (6)

In the method of extracting resveratrol from grapes, a) drying the grape cluster branches; b) grinding the grape branch to a particle size of 0.3-0.5 mm to obtain a pulverized product; c) injecting 5-10 L of water and ethanol mixed at a volume basis of 2: 8-3: 7 per kg of the ground powder; And d) adding the solvent into which the pulverized powder is added in a range of 45-50 kHz ultrasonically at room temperature, at an energy intensity of 0.03-0.05 kW per 1 L of the solvent into which the pulverized powder is added. The method of claim 1 wherein said drying is accomplished by sunlight drying for about 30 days. delete delete delete delete