CN113186181A - 黄嘌呤酰胺水解酶及其用途 - Google Patents

Abstract

本申请提供了黄嘌呤酰胺水解酶及其用途，特别是在治疗痛风中的用途。

Description

黄嘌呤酰胺水解酶及其用途

技术领域

本申请大体涉及生物医药技术领域；具体而言，本申请提供了新的嘌呤降解通路、参与该通路的酶及它们的用途，特别是在治疗痛风中的应用。

背景技术

嘌呤的吸收主要发生在肠道，肠道为无氧环境。痛风是嘌呤代谢异常导致的一种关节炎，其特征是反复发作的红色、触痛、灼热和肿胀的关节。疼痛通常很快发生，在不到12小时内达到最大强度。造成痛风的原因是体内尿酸水平的升高，造成了尿酸盐在关节和肾脏部位的沉积，而尿酸是嘌呤代谢的产物。

目前已有的治疗痛风的药物有秋水仙碱、别嘌呤醇、非布司他以及其它非甾体类抗炎药和糖皮质激素等。秋水仙碱通过抑制中性白细胞的趋化、黏附和吞噬作用等，从而达到控制关节局部的疼痛、肿胀及炎症反应。别嘌呤醇和非布司他为选择性黄嘌呤氧化酶抑制剂，可通过降低血尿酸盐浓度而发挥治疗痛风的作用，但以上药物均有较大的副作用。

新的用于预防、干预和/或治疗痛风的药物的开发和应用是本领域所需要的。

发明概述

第一方面，本申请提供了多肽，其包含SEQ ID NO:1所示的氨基酸序列或其功能变体，其中所述功能变体具有黄嘌呤酰胺水解酶活性。

在第一方面的一些实施方案中，其中所述多肽在空间构象上具有如下定义的催化部位：

所述催化部位包含空间构象上相互靠近的、参照SEQ ID NO:1的H59、H61、K151、H186、H242和D316氨基酸残基。

在第一方面的一些实施方案中，所述催化部位还包含二价金属离子。

在第一方面的一些实施方案中，所述多肽还包含在空间构象上具有如下定义的结合部位：

所述结合部位包含在空间构象上相互靠近的、参照SEQ ID NO:1的I288、A289、P338和G339氨基酸残基。

在第一方面的一些实施方案中，其中所述催化部位与所述结合部位相距不大于5埃

。

在第一方面的一些实施方案中，所述功能变体为SEQ ID NO:1所示的氨基酸序列的天然同工酶。

在第一方面的一些实施方案中，其中所述功能变体为在SEQ ID NO:1所示的氨基酸序列或其天然同工酶的基础上发生一个或多个氨基酸的插入、取代和/或缺失而产生的。

在第一方面的一些实施方案中，其中所述插入、取代和/或缺失不发生在催化部位和/或结合部位。

第二方面，本申请提供了核酸分子，其编码第一方面所述的多肽。

第三方面，本申请提供了表达盒，其包含第二方面所述的核酸分子。

第四方面，本申请提供了表达载体，其包含第二方面所述的核酸分子或第三方面所述的表达盒。

第五方面，本申请提供了宿主细胞，其包含第二方面所述的核酸分子、第三方面所述的表达盒或第四方面所述的表达载体。

在第五方面的一些实施方案中，所述宿主细胞能够表达并产生：多肽，其包含SEQID NO:1所示的氨基酸序列或其功能变体，其中所述功能变体具有黄嘌呤酰胺水解酶活性。

在第五方面的一些实施方案中，其中所述多肽在空间构象上具有如下定义的催化部位：

所述催化部位包含空间构象上相互靠近的、参照SEQ ID NO:1的H59、H61、K151、H186、H242和D316氨基酸残基。

在第五方面的一些实施方案中，所述催化部位还包含二价金属离子。

在第五方面的一些实施方案中，所述宿主细胞为真核细胞或原核细胞。

在第五方面的一些实施方案中，所述真核细胞为酵母细胞。

在第五方面的一些实施方案中，所述原核细胞选自埃希氏菌属、乳酸杆菌属、双歧杆菌属、拟杆菌门和厚壁菌门

第六方面，本申请提供了药物组合物或保健食品，其包含第一方面所述的多肽、第二方面所述的核酸分子、第三方面所述的表达盒、第四方面所述的表达载体或第五方面所述的宿主细胞和药学上可接受的载体或赋形剂。

在第六方面的一些实施方案中，所述药物组合物或保健食品用于预防和/或干预和/或治疗痛风。

第七方面，本申请提供了第一方面所述的多肽、第二方面所述的核酸分子、第三方面所述的表达盒、第四方面所述的表达载体、第五方面所述的宿主细胞或第六方面所述的药物组合物或保健食品在降解嘌呤中的用途。

在第七方面的一些实施方案中，所述降解嘌呤在体外发生。

第八方面，本申请提供了第一方面所述的多肽、第二方面所述的核酸分子、第三方面所述的表达盒、第四方面所述的表达载体、第五方面所述的宿主细胞或第六方面所述的药物组合物或保健食品在制备用于预防、干预和/或治疗痛风的药物中的用途。

第九方面，本申请提供了预防、干预和/或治疗痛风的方法，其包括向有需要的个体给予第一方面所述的多肽、第二方面所述的核酸分子、第三方面所述的表达盒、第四方面所述的表达载体、第五方面所述的宿主细胞或第六方面所述的药物组合物或保健食品。

附图说明

图1为人体降解各种嘌呤的代谢通路及催化每一步反应的酶的EC编号。

图2为黄嘌呤氧化酶降解黄嘌呤产生尿酸的代谢反应及其涉及复杂含Mo辅基的催化机理。

图3为别嘌呤醇通过抑制黄嘌呤氧化酶阻止尿酸生成的治疗痛风的机理。

图4为黄嘌呤酰胺水解酶在无氧条件下降解黄嘌呤的反应式(1)及整个代谢通路。

图5为纯化后的黄嘌呤酰胺水解酶的电泳结果，其中泳道M代表分子量标识，泳道T为表达黄嘌呤酰胺水解酶的细菌裂解液，泳道U代表为经Co亲和柱后的细菌裂解液，泳道E₁、E₂、E₃分别代表经钴亲和柱纯化洗脱的1、2和4μg黄嘌呤酰胺水解酶。

图6为黄嘌呤酰胺水解酶催化黄嘌呤水解开环的酶活性LC-MS(液质联用)检测结果，其中A为不加酶的阴性对照的LC-MS检测结果，B为实验组的LC-MS检测结果。

图7为经PHYRE2服务器(PHYRE2 Protein Fold Recognition Server)进行计算机模拟得到的黄嘌呤酰胺水解酶结构的活性中心的图，其中催化部位包含空间构象上相互靠近的4个组氨酸、一个羧基化的赖氨酸、一个天冬氨酸以及两个二价金属离子(例如Zn²⁺和/或Mn²⁺)；结合部位包含空间构象上相互靠近的与黄嘌呤相互作用的一个异亮氨酸、一个丙氨酸、一个脯氨酸和一个甘氨酸残基。

图8为UniRef50_Q3AEA1所有黄嘌呤酰胺水解酶的进化系统树图。灰色标记为在各树状分支中选取菌株代表坚强芽孢杆菌(Bacillus firmus)、柱胞梭菌DSM 605(Clostridium cylindrosporum DSM 605)、Clostridium purinilyticum、产氢羧基嗜热菌(Carbydothermus hydrogenoformans，菌株ATCC BAA-161/DSM 6008/Z-2901)以及Paenibacillus donghaensis，将它们编码的登录号分别为A0A366K523、A0A0J8G334、A0A0L0W692、Q3AEA1和A0A2Z2KEH1的黄嘌呤酰胺水解酶的氨基酸序列在图9中进行序列对比。

图9为五个不同物种坚强芽孢杆菌(Bacillus firmus)、柱胞梭菌DSM 605(Clostridium cylindrosporum DSM 605)、Clostridium purinilyticum、产氢羧基嗜热菌(Carbydothermus hydrogenoformans，菌株ATCC BAA-161/DSM 6008/Z-2901)以及Paenibacillus donghaensis，编码的登录号分别为A0A366K523、A0A0J8G334、A0A0L0W692、Q3AEA1和A0A2Z2KEH1的黄嘌呤酰胺水解酶的序列对比结果，其中催化部位包含两个二价金属(例如Zn²⁺或Mn²⁺等)及与其配位的背景为灰色的四个组氨酸(H)、一个赖氨酸(K)和一个天冬氨酸(D)残基(实线方框)；结合部位包含与黄嘌呤结合的背景为灰色的一个异亮氨酸(I)、一个丙氨酸(A)、一个脯氨酸(P)和一个甘氨酸残基(G)。

序列说明

SEQ ID NO：1显示了坚强芽孢杆菌(Bacillus firmus，CGMCC 1.2010)表达的黄嘌呤酰胺水解酶的氨基酸序列。

SEQ ID NO：2显示了用于PCR扩增编码黄嘌呤酰胺水解酶的核酸分子的上游引物的核苷酸序列。

SEQ ID NO：3显示了用于PCR扩增编码黄嘌呤酰胺水解酶的核酸分子的下游引物的核苷酸序列。

SEQ ID NO：4显示了Clostridium purinilytica表达的登录号为A0A0L0W692的黄嘌呤酰胺水解酶的氨基酸序列。

SEQ ID NO：5显示了高温黄色微球菌FBKL4.011(Thermoflavimicrobiumsp.FBKL4.011)表达的登录号为A0A364K317的黄嘌呤酰胺水解酶的氨基酸序列。

SEQ ID NO：6显示了耐盐海洋丝状菌(Marininema halotolerans)表达的登录号为A0A1I6SUY8的黄嘌呤酰胺水解酶的氨基酸序列。

SEQ ID NO：7显示了梭菌科细菌(Clostridiaceae bacterium)表达的登录号为A0A3D2NSM2的黄嘌呤酰胺水解酶的氨基酸序列。

SEQ ID NO：8显示了芽孢杆菌2_A_57_CT2(Bacillus sp.2_A_57_CT2)表达的登录号为E5WNF6的黄嘌呤酰胺水解酶的氨基酸序列。

SEQ ID NO：9显示了类芽孢杆菌FSL H8-0259(Paenibacillus sp.FSL H8-0259)表达的登录号为A0A1R1CK18的黄嘌呤酰胺水解酶的氨基酸序列。

SEQ ID NO：10显示了双岐高温黄色微球菌(Thermoflavimicrobiumdichotomicum)表达的登录号为A0A1I3U261的黄嘌呤酰胺水解酶的氨基酸序列。

SEQ ID NO：11显示了Fictibacillus enclensis表达的登录号为A0A0V8JCN2的黄嘌呤酰胺水解酶的氨基酸序列。

SEQ ID NO：12显示了中温海洋丝状菌(Marininema mesophilum)表达的登录号为A0A1H2QVP9的黄嘌呤酰胺水解酶的氨基酸序列。

SEQ ID NO：13显示了类芽孢杆菌FSL R5-0912(Paenibacillus sp.FSL R5-0912)表达的登录号为A0A089K5P4的黄嘌呤酰胺水解酶的氨基酸序列。

SEQ ID NO：14显示了香蒲类芽孢杆菌(Paenibacillus typhae)表达的登录号为A0A1G9DGW2的黄嘌呤酰胺水解酶的氨基酸序列。

SEQ ID NO：15显示了极尖组织菌(Tissierella praeacuta)DSM18095表达的登录号为A0A1M4UHZ6的黄嘌呤酰胺水解酶的氨基酸序列。

SEQ ID NO：16显示了芽孢杆菌cl95(Bacillus sp.cl95)表达的登录号为A0A1I6C7J4的黄嘌呤酰胺水解酶的氨基酸序列。

SEQ ID NO：17显示了慢生型大豆根瘤菌(Bradyrhizobium japonicum)表达的登录号为A0A0A3XEE6的黄嘌呤酰胺水解酶的氨基酸序列。

SEQ ID NO：18显示了坚强芽孢杆菌(Bacillus firmus)表达的登录号为A0A380XTT6的黄嘌呤酰胺水解酶的氨基酸序列。

SEQ ID NO：19显示了产氢氨基酸杆菌(Acidaminobacter hydrogenoformans)DSM2784表达的登录号为A0A1G5S5P6的黄嘌呤酰胺水解酶的氨基酸序列。

SEQ ID NO：20显示了温铁还原细菌TR13(Caloranaerobacter sp.TR13)表达的登录号为A0A0P8Z9T7的黄嘌呤酰胺水解酶的氨基酸序列。

SEQ ID NO：21显示了组织菌P1(Tissierella sp.P1)表达的登录号为A0A265Q2B2的黄嘌呤酰胺水解酶的氨基酸序列。

SEQ ID NO：22显示了芽孢杆菌3-2-2(Bacillus sp.3-2-2)表达的登录号为A0A429Y566的黄嘌呤酰胺水解酶的氨基酸序列。

SEQ ID NO：23显示了Paenibacillus donghaensis表达的登录号为A0A2Z2KEH1的黄嘌呤酰胺水解酶的氨基酸序列。

SEQ ID NO：24显示了Gottschalkia acidurici(菌株ATCC7906/DSM 604/BCRC14475/CIP 104303/NCIMB 10678/9a)表达的登录号为K0AWA5的黄嘌呤酰胺水解酶的氨基酸序列。

SEQ ID NO：25显示了强壮芽孢杆菌(Bacillus fortis)表达的登录号为A0A443IKX3的黄嘌呤酰胺水解酶的氨基酸序列。

SEQ ID NO：26显示了海泥芽孢杆菌(Bacillus oceanisediminis)表达的登录号为A0A2V2ZNB0的黄嘌呤酰胺水解酶的氨基酸序列。

SEQ ID NO：27显示了Virgibacillus profundi表达的登录号为A0A2A2IEE5的黄嘌呤酰胺水解酶的氨基酸序列。

SEQ ID NO：28显示了类芽孢杆菌FSL R7-0331(Paenibacillus sp.FSL R7-0331)表达的登录号为A0A089MDW3的黄嘌呤酰胺水解酶的氨基酸序列。

SEQ ID NO：29显示了芽孢杆菌FJAT-21945(Bacillus sp.FJAT-21945)表达的登录号为A0A0M0X8C9的黄嘌呤酰胺水解酶的氨基酸序列。

SEQ ID NO：30显示了极尖组织菌(Tissierella praeacuta)表达的登录号为A0A3F3S6I2的黄嘌呤酰胺水解酶的氨基酸序列。

SEQ ID NO：31显示了海泥芽孢杆菌(Bacillus oceanisediminis)表达的登录号为A0A1S1YDG4的黄嘌呤酰胺水解酶的氨基酸序列。

SEQ ID NO：32显示了芽孢杆菌OV194(Bacillus sp.OV194)表达的登录号为A0A1I1X526的黄嘌呤酰胺水解酶的氨基酸序列。

SEQ ID NO：33显示了Anaeromicrobium sediminis表达的登录号为A0A267MJF0的黄嘌呤酰胺水解酶的氨基酸序列。

SEQ ID NO：34显示了解脂嗜热互营杆菌(Thermosyntropha lipolytica)DSM11003表达的登录号为A0A1M5RDL0的黄嘌呤酰胺水解酶的氨基酸序列。

SEQ ID NO：35显示了Alkaliphilus peptidifermentans DSM 18978表达的登录号为A0A1G5KXH0的黄嘌呤酰胺水解酶的氨基酸序列。

SEQ ID NO：36显示了米氏解硫胺素芽孢杆菌(Aneurinibacillus migulanus)表达的登录号为A0A1G8Q7H9的黄嘌呤酰胺水解酶的氨基酸序列。

SEQ ID NO：37显示了类芽孢杆菌IHB B 3415(Paenibacillus sp.IHB B 3415)表达的登录号为A0A0B2F3Z2的黄嘌呤酰胺水解酶的氨基酸序列。

SEQ ID NO：38显示了Marinisporobacter balticus表达的登录号为A0A4R2KME8的黄嘌呤酰胺水解酶的氨基酸序列。

SEQ ID NO：39显示了土地芽孢杆菌(Bacillus terrae)表达的登录号为A0A429X0W8的黄嘌呤酰胺水解酶的氨基酸序列。

SEQ ID NO：40显示了加利福尼亚丁达尔氏菌(Tindallia californiensis)表达的登录号为A0A1H3Q6T8的黄嘌呤酰胺水解酶的氨基酸序列。

SEQ ID NO：41显示了Clostridium acidurici(菌株ATCC7906/DSM 604/BCRC14475/CIP 104303/NCIMB 10678/9a)表达的登录号为K0B360的黄嘌呤酰胺水解酶的氨基酸序列。

SEQ ID NO：42显示了Romboutsia lituseburensis DSM 797表达的登录号为A0A1G9M635的黄嘌呤酰胺水解酶的氨基酸序列。

SEQ ID NO：43显示了Paraclostridium bifermentans ATCC 19299表达的登录号为T4V5T0的黄嘌呤酰胺水解酶的氨基酸序列。

SEQ ID NO：44显示了Bacillus praedii表达的登录号为A0A4R1ATL6的黄嘌呤酰胺水解酶的氨基酸序列。

SEQ ID NO：45显示了Carbydothermus islandicus表达的登录号为A0A1L8D5S0的黄嘌呤酰胺水解酶的氨基酸序列。

SEQ ID NO：46显示了Caloramator australicus RC3表达的登录号为I7KTT3的黄嘌呤酰胺水解酶的氨基酸序列。

SEQ ID NO：47显示了Paraclostridium bifermentans ATCC 638表达的登录号为T4VRB8的黄嘌呤酰胺水解酶的氨基酸序列。

SEQ ID NO：48显示了Tepidimicrobium xylanilyticum表达的登录号为A0A1H2RLU1的黄嘌呤酰胺水解酶的氨基酸序列。

SEQ ID NO：49显示了米氏解硫胺素芽孢杆菌(Aneurinibacillus migulanus)表达的登录号为A0A0K2WJ73的黄嘌呤酰胺水解酶的氨基酸序列。

SEQ ID NO：50显示了芽孢杆菌属(Bacillus bacterium)表达的登录号为A0A3D0EBN7的黄嘌呤酰胺水解酶的氨基酸序列。

SEQ ID NO：51显示了Bacillus notoginsengisoli表达的登录号为A0A417YW08的黄嘌呤酰胺水解酶的氨基酸序列。

SEQ ID NO：52显示了海泥芽孢杆菌2691(Bacillus oceanisediminis 2691)表达的登录号为A0A160MBB4的黄嘌呤酰胺水解酶的氨基酸序列。

SEQ ID NO：53显示了类芽孢杆菌FSL R7-0273(Paenibacillus sp.FSL R7-0273)表达的登录号为A0A089LXU3的黄嘌呤酰胺水解酶的氨基酸序列。

SEQ ID NO：54显示了[Clostridium]ultunense Esp表达的登录号为M1ZGS5的黄嘌呤酰胺水解酶的氨基酸序列。

SEQ ID NO：55显示了Bacillus freudenreichii表达的登录号为A0A448FF64的黄嘌呤酰胺水解酶的氨基酸序列。

SEQ ID NO：56显示了Caloramator fervidus表达的登录号为A0A1H5RUL9的黄嘌呤酰胺水解酶的氨基酸序列。

SEQ ID NO：57显示了海泥芽孢杆菌(Bacillus oceanisediminis)表达的登录号为A0A4R8GVS7的黄嘌呤酰胺水解酶的氨基酸序列。

SEQ ID NO：58显示了非解糖卟啉单胞菌(Soehngenia saccharolytica)表达的登录号为A0A4T9ZWH0的黄嘌呤酰胺水解酶的氨基酸序列。

SEQ ID NO：59显示了坚强芽孢杆菌DS1(Bacillus firmus DS1)表达的登录号为W7LB72的黄嘌呤酰胺水解酶的氨基酸序列。

SEQ ID NO：60显示了Thermotalea metallivorans表达的登录号为A0A140L3I5的黄嘌呤酰胺水解酶的氨基酸序列。

SEQ ID NO：61显示了Ornithinibacillus halophilus表达的登录号为A0A1M5FNK3的黄嘌呤酰胺水解酶的氨基酸序列。

SEQ ID NO：62显示了解脂嗜热互营杆菌(Thermosyntropha lipolytica)DSM11003表达的登录号为A0A1M5LQE8的黄嘌呤酰胺水解酶的氨基酸序列。

SEQ ID NO：63显示了虚构芽孢杆菌S7(Fictibacillus sp.S7)表达的登录号为A0A4Q2HRQ1的黄嘌呤酰胺水解酶的氨基酸序列。

SEQ ID NO：64显示了Bacillus mesonae表达的登录号为A0A3Q9QZ31的黄嘌呤酰胺水解酶的氨基酸序列。

SEQ ID NO：65显示了Clostridium purinilytica表达的登录号为A0A0L0W688的黄嘌呤酰胺水解酶的氨基酸序列。

SEQ ID NO：66显示了Tindallia magadiensis表达的登录号为A0A1I3ETA9的黄嘌呤酰胺水解酶的氨基酸序列。

SEQ ID NO：67显示了北拟杆菌(Paenibacillus borealis)表达的登录号为A0A089LHF8的黄嘌呤酰胺水解酶的氨基酸序列。

SEQ ID NO：68显示了Paraclostridium benzoelyticum表达的登录号为A0A0M3DN30的黄嘌呤酰胺水解酶的氨基酸序列。

SEQ ID NO：69显示了Bacillus solani表达的登录号为A0A0Q3QMR7的黄嘌呤酰胺水解酶的氨基酸序列。

SEQ ID NO：70显示了Thermohalobacter berrensis表达的登录号为A0A419T2I0的黄嘌呤酰胺水解酶的氨基酸序列。

SEQ ID NO：71显示了不动杆菌RIT592(Acinetobacter sp.RIT592)表达的登录号为A0A369PBX6的黄嘌呤酰胺水解酶的氨基酸序列。

SEQ ID NO：72显示了Maledivibacter halophilus表达的登录号为A0A1T5JUM9的黄嘌呤酰胺水解酶的氨基酸序列。

SEQ ID NO：73显示了Tissierella creatinini表达的登录号为A0A4T9WHZ3的黄嘌呤酰胺水解酶的氨基酸序列。

SEQ ID NO：74显示了堆肥宏基因组(compost metagenome)表达的登录号为A0A3R1HSK2的黄嘌呤酰胺水解酶的氨基酸序列。

SEQ ID NO：75显示了Natronincola peptidivorans表达的登录号为A0A1I0F3P2的黄嘌呤酰胺水解酶的氨基酸序列。

SEQ ID NO：76显示了坚强芽孢杆菌(Bacillus firmus)表达的登录号为A0A0J5YTU9的黄嘌呤酰胺水解酶的氨基酸序列。

SEQ ID NO：77显示了Anaerovirgula multivorans表达的登录号为A0A239FV01的黄嘌呤酰胺水解酶的氨基酸序列。

SEQ ID NO：78显示了产氢羧基嗜热菌(Carbydothermus hydrogenoformans，菌株ATCC BAA-161/DSM 6008/Z-2901)表达的登录号为Q3AEA1的黄嘌呤酰胺水解酶的氨基酸序列。

SEQ ID NO：79显示了产乙酸互营单包菌DSM13106(Sporanaerobacteracetigenes)DSM 13106表达的登录号为A0A1M5YST8的黄嘌呤酰胺水解酶的氨基酸序列。

SEQ ID NO：80显示了Proteiniborus sp.DW1表达的登录号为A0A1M4MA63的黄嘌呤酰胺水解酶的氨基酸序列。

SEQ ID NO：81显示了芽孢杆菌7894-2(Bacillus sp.7894-2)表达的登录号为A0A268IWM5的黄嘌呤酰胺水解酶的氨基酸序列。

SEQ ID NO：82显示了[Clostridium]ultunense Esp表达的登录号为A0A1M4PLJ1的黄嘌呤酰胺水解酶的氨基酸序列。

SEQ ID NO：83显示了Virgibacillus indicus表达的登录号为A0A265N7L4的黄嘌呤酰胺水解酶的氨基酸序列。

SEQ ID NO：84显示了类芽孢杆菌NFR01(Paenibacillus sp.NFR01)表达的登录号为A0A1I0KAI3的黄嘌呤酰胺水解酶的氨基酸序列。

SEQ ID NO：85显示了Andreesenia angusta表达的登录号为A0A1S1V3Q5的黄嘌呤酰胺水解酶的氨基酸序列。

SEQ ID NO：86显示了类芽孢杆菌DMB5(Paenibacillus sp.DMB5)表达的登录号为A0A117T0P2的黄嘌呤酰胺水解酶的氨基酸序列。

SEQ ID NO：87显示了Paludifilum halophilum表达的登录号为A0A235B9X8的黄嘌呤酰胺水解酶的氨基酸序列。

SEQ ID NO：88显示了Proteiniborus ethanoligenes表达的登录号为A0A1H3NJW1的黄嘌呤酰胺水解酶的氨基酸序列。

SEQ ID NO：89显示了Alkaliphilus metalliredigens(菌株QYMF)表达的登录号为A6TWT7的黄嘌呤酰胺水解酶的氨基酸序列。

SEQ ID NO：90显示了盐渍土假芽胞杆菌(Fictibacillus solisalsi)表达的登录号为A0A1G9U6Z5的黄嘌呤酰胺水解酶的氨基酸序列。

SEQ ID NO：91显示了Sporosarcina globispora表达的登录号为A0A0M0GGH4的黄嘌呤酰胺水解酶的氨基酸序列。

SEQ ID NO：92显示了坚强芽孢杆菌(Bacillus firmus)表达的登录号为A0A366K523的黄嘌呤酰胺水解酶的氨基酸序列。

SEQ ID NO：93显示了类芽孢杆菌FSL R5-0490(Paenibacillus sp.FSL R5-0490)表达的登录号为A0A1R1FFH4的黄嘌呤酰胺水解酶的氨基酸序列。

SEQ ID NO：94显示了嗜碱菌(Alkaliphilus sp.)表达的登录号为A0A2G2MLE4的黄嘌呤酰胺水解酶的氨基酸序列。

SEQ ID NO：95显示了厌氧砷还原菌(Alkaliphilus oremlandii，菌株OhILAs)表达的登录号为A8MLA7的黄嘌呤酰胺水解酶的氨基酸序列。

SEQ ID NO：96显示了Caloramator mitchellensis表达的登录号为A0A0R3JVG6的黄嘌呤酰胺水解酶的氨基酸序列。

SEQ ID NO：97显示了柱胞梭菌DSM 605(Clostridiumcylindrosporum DSM 605)表达的登录号为A0A0J8G334的黄嘌呤酰胺水解酶的氨基酸序列。

SEQ ID NO：98显示了嗜氨菌CFH 90114(Ammoniphilus sp.CFH90114)表达的登录号为A0A4Q1SWC2的黄嘌呤酰胺水解酶的氨基酸序列。

SEQ ID NO：99显示了Carbydothermus pertinax表达的登录号为A0A1L8CSM0的黄嘌呤酰胺水解酶的氨基酸序列。

SEQ ID NO：100显示了Soehngenia sp.1933P表达的登录号为A0A4Z0D783的黄嘌呤酰胺水解酶的氨基酸序列。

SEQ ID NO：101显示了非解糖卟啉单胞菌(Soehngenia saccharolytica)表达的登录号为A0A4T9ZOT2的黄嘌呤酰胺水解酶的氨基酸序列。

SEQ ID NO：102显示了Natribacillus halophilus表达的登录号为A0A1G8N2H8的黄嘌呤酰胺水解酶的氨基酸序列。

SEQ ID NO：103显示了堆肥宏基因组(compost metagenome)表达的登录号为A0A403WBU6的黄嘌呤酰胺水解酶的氨基酸序列。

SEQ ID NO：104显示了堆肥宏基因组(compost metagenome)表达的登录号为A0A3R4A6V9的黄嘌呤酰胺水解酶的氨基酸序列。

发明详细描述

图1显示了人体中各种嘌呤的代谢通路，其中各种嘌呤代谢的终产物为尿酸盐，而尿酸盐在关节和肾脏等部位的沉积是导致痛风的原因。图2显示了需氧型蛋白黄嘌呤氧化酶催化黄嘌呤降解生成尿酸的过程。治疗痛风的药物别嘌呤醇通过抑制黄嘌呤氧化酶来抑制尿酸的产生，从而治疗痛风(图3)。G.D.VOGELS等人(Degradation of Purines andPyrimidines by Microorganisms,Bacteriological Reviews,June 1976,Vol.40,No.2,p.403-468)报道了柱胞梭菌(Clostridium cylindrosporum)在无氧条件下可以将黄嘌呤降解为亚胺甲基甘氨酸，图4显示了黄嘌呤酰胺水解酶在无氧条件下降解黄嘌呤的反应式。本申请的发明人意识到无氧条件下的嘌呤降解具有重要意义，因为人体的肠道为无氧环境，如果能先于肠道对嘌呤的吸收，在肠道内降解嘌呤，这对于预防、干预、缓解和/或治疗痛风是一种新思路。本申请的发明人利用生物信息学的方法在进行了大量工作后，成功地鉴定出无氧条件下降解黄嘌呤的黄嘌呤酰胺水解酶。

除非另外指明，本申请中所用的术语具有本领域技术人员通常所理解的含义。

定义

除非另有所指，分别地，核酸以5'至3'方向从左向右书写；氨基酸序列以氨基至羧基方向从左向右书写。数字范围包括限定该范围的数字。氨基酸在本文可以用其通常所知的三字母符号或IUPAC-IUB生物化学命名委员会推荐的一字母符号来表示。同样地，可以用通常接受的单字母码表示核苷酸。参考说明书整体更为充分地定义以上定义的术语。

本申请的“多肽”和“蛋白”在本文可互换使用，指氨基酸残基的聚合物及其变体和合成的和天然存在的类似物。因此，这些术语适用于天然存在的氨基酸聚合物及其天然存在的化学衍生物，以及其中一个或多个氨基酸残基是合成的非天然存在的氨基酸(诸如相应的天然存在的氨基酸的化学类似物)的氨基酸聚合物。此类衍生物包括例如翻译后修饰和降解产物，包括多肽片段的磷酸化的、糖基化的、氧化的、异构化、羧基化的和脱氨基化的变体。

如本文所用的术语“酶活性中心”是指酶分子中能够直接与底物分子结合，并催化底物化学反应的部位，这一部位就成为酶的活性中心。一般认为活性中心主要由两个功能部位组成：第一个是催化部位，底物的键在此被打断或形成新的键从而发生一定的化学变化；第二个是结合部位，酶的底物靠此部位结合到酶分子上。组成功能部位的是酶分子中在三维结构上比较靠近的少数几个氨基酸残基或是这些残基上的某些基团，它们在一级结构上可能相距甚远，甚至位于不同肽链上，而是通过肽链的盘绕、折叠在空间构象上相互靠近；对于需要辅酶的酶来说，辅酶分子(如金属离子Zn²⁺和/或Mn²⁺)或辅酶分子的某一部分结构也是功能部位的组成部分。

如本文所用的术语“氨基酸”是指羧酸碳原子上的氢原子被氨基取代后的化合物，氨基酸分子中含有氨基和羧基两种官能团。其包括天然存在的和非天然存在的氨基酸以及氨基酸类似物和模拟物。天然存在的氨基酸包括蛋白生物合成中使用的20种(L)-氨基酸以及其他氨基酸，例如4-羟脯氨酸、羟赖氨酸、羧化赖氨酸、锁链素、异锁链素、高半胱氨酸、瓜氨酸和鸟氨酸。非天然存在的氨基酸包括例如(D)-氨基酸、正亮氨酸、正缬氨酸、p-氟苯丙氨酸、乙基硫氨酸等，这些是本领域技术人员已知的。氨基酸类似物包括天然和非天然存在的氨基酸的修饰形式。这种修饰可以包括例如取代氨基酸上的化学基团和部分，或者氨基酸的衍生化。氨基酸模拟物包括例如表现出功能上类似性质的有机结构，所述性质例如氨基酸的电荷和电荷空间特性。例如，模拟精氨酸(Arg或R)的有机结构具有位于类似分子空间并且具有与天然存在的Arg氨基酸的侧链的e-氨基相同程度的移动性的正电荷部分。模拟物还包括约束结构以维持氨基酸或氨基酸官能团的最佳空间和电荷相互作用。本领域技术人员可以确定什么结构构成功能上等效的氨基酸类似物和氨基酸模拟物。

如本文所用的术语“同工酶”是指生物体内催化相同反应而分子结构不同的酶。

如本文所用的术语“核酸”是指指mRNA、RNA、cRNA、cDNA或DNA，包括单链和双链形式的DNA。该术语通常指至少10个碱基长度的核苷酸多聚形式，所述核苷酸是核糖核苷酸或脱氧核苷酸或任一类型的核苷酸的修饰形式。

如本文所用的术语“编码”用于特定核酸的上下文时，指该核酸包含指导该核苷酸序列翻译成特定蛋白的必需信息。使用密码子表示编码蛋白的信息。编码蛋白的核酸可以包含位于该核酸翻译区内的非翻译序列(例如，内含子)或者可以缺少这样的居间非翻译序列(例如，如同在cDNA中)。

如本文所用的，涉及特定多核苷酸或其所编码的蛋白的“全长序列”指具有天然(非合成)内源序列的整个核酸序列或整个氨基酸序列。全长多核苷酸编码该特定蛋白的全长、催化活性形式。

如本文所用的术语“分离的”是指多肽或核酸或其生物学活性部分，其基本上或本质上不含如在其天然存在的环境中所发现的通常伴随或反应于该蛋白质或核酸的组分。因而，用重组技术产生分离的多肽或核酸时，分离的多肽或核酸基本上不含其它细胞物质或培养基，或者化学合成分离的多肽或核酸时，基本上不含化学前体或其它化学品。

如本文所用的术语“表达载体”是重组或合成产生的核酸构建体，其具有一系列允许特定的核酸在宿主细胞中转录的特异性核酸元件。

如本文所用的术语“宿主细胞”是指在转化和转导(感染)中接受外源基因的细胞。宿主细胞可以是诸如酵母细胞的真核细胞或诸如大肠杆菌的原核细胞。

具体实施方案

第一方面，本申请提供了多肽，其包含SEQ ID NO:1所示的氨基酸序列或其功能变体，其中所述功能变体具有黄嘌呤酰胺水解酶活性。

在第一方面的一些实施方案中，其在空间构象上具有如下定义的催化部位：

所述催化部位包含空间构象上相互靠近的、参照SEQ ID NO:1的H59、H61、K151、H186、H242和D316氨基酸残基。

在第一方面的一些实施方案中，所述催化部位还包含二价金属离子。

在第一方面的一些具体实施方案中，所述催化部位还包含2个二价金属离子，例如Zn²⁺和/或Mn²⁺。

在第一方面的一些实施方案中，其中所述多肽还包含在空间构象上具有如下定义的结合部位：

所述结合部位包含在空间构象上相互靠近的、参照SEQ ID NO:1的I288、A289、P338和G339氨基酸残基。

在第一方面的一些实施方案中，所述催化部位与所述结合部位相距不大于5埃

。

在第一方面的一些实施方案中，其中所述功能变体为SEQ ID NO:1所示的氨基酸序列的天然同工酶。

在第一方面的一些实施方案中，所述天然同工酶来自：坚强芽孢杆菌(Bacillusfirmus)、堆肥宏基因组(compost metagenome)、Clostridium purinilyticum、高温黄色微球菌(Thermoflavimicrobium sp.)、耐盐海洋丝状菌(Marininema halotolerans)、梭菌科细菌(Clostridiaceae bacterium)、芽孢杆菌(Bacillus sp.)、类芽孢杆菌(Paenibacillus sp.)、双岐高温黄色微球菌(Thermoflavimicrobium dichotomicum)、Fictibacillus enclensis、中温海洋丝状菌(Marininema mesophilum)、香蒲类芽孢杆菌(Paenibacillus typhae)、极尖组织菌(Tissierella praeacuta)、慢生型大豆根瘤菌(Bradyrhizobium japonicum)、产氢氨基酸杆菌(Acidaminobacter hydrogenoformans)、温铁还原细菌(Caloranaerobacter sp.)、组织菌(Tissierella sp.)、Paenibacillusdonghaensis、Gottschalkia acidurici、Clostridium acidurici、强壮芽孢杆菌(Bacillus fortis)、海泥芽孢杆菌(Bacillus oceanisediminis)、Virgibacillusprofundi、Anaeromicrobium sediminis、解脂嗜热互营杆菌(Thermosyntrophalipolytica)、Alkaliphilus peptidifermentans、米氏解硫胺素芽孢杆菌(Aneurinibacillus migulanus)、芽孢杆菌细菌属(Bacillus bacterium)、Marinisporobacter balticus、土地芽孢杆菌(Bacillus terrae)、加利福尼亚丁达尔氏菌(Tindallia californiensis)、Romboutsia lituseburensis、Paraclostridiumbifermentans、Bacillus praedii、Carbydothermus islandicus、Caloramatoraustralicus、Paraclostridium bifermentans、Tepidimicrobium xylanilyticum、Bacillus notoginsengisoli、[Clostridium]ultunense Esp、Bacillus freudenreichii、Caloramator fervidus、非解糖卟啉单胞菌(Soehngenia saccharolytica)、Thermotaleametallivorans、Ornithinibacillus halophilus、虚构芽孢杆菌(Fictibacillus sp.)、Bacillus mesonae、Tindallia magadiensis、北拟杆菌(Paenibacillus borealis)、Paraclostridium benzoelyticum、Bacillus solani、Thermohalobacter berrensis、不动杆菌(Acinetobacter sp.)、Maledivibacter halophilus、Tissierella creatinini、Natronincola peptidivorans、Anaerovirgula multivorans、产氢羧基嗜热菌(Carbydothermus hydrogenoformans)、产乙酸互营单包菌(Sporanaerobacteracetigenes)、Proteiniborus sp.、Virgibacillus indicus、Andreesenia angusta、Paludifilum halophilum、Proteiniborus ethanoligenes、Alkaliphilusmetalliredigens、盐渍土假芽胞杆菌(Fictibacillus solisalsi)、Sporosarcinaglobispora、嗜碱菌(Alkaliphilus sp.)、厌氧砷还原菌(Alkaliphilus oremlandii)、Caloramator mitchellensis、柱胞梭菌(Clostridium cylindrosporum)、嗜氨菌(Ammoniphilus sp.)、Carbydothermus pertinax、Soehngenia sp.和Natribacillushalophilus。

在第一方面的一些具体实施方案中，所述天然同工酶包含SEQ ID NO:4-104中任一项所示的氨基酸序列。

在第一方面的一些具体实施方案中，其中所述功能变体为在SEQ ID NO:1所示的氨基酸序列或其天然同工酶的基础上发生一个或多个氨基酸的插入、取代和/或缺失而产生的。

在第一方面的一些实施方案中，所述插入、取代和/或缺失不发生在催化部位。

在第一方面的一些实施方案中，所述插入、取代和/或缺失不发生在结合部位。

在第一方面的一些实施方案中，所述插入、取代和/或缺失不发生在催化部位和结合部位。

在第一方面的一些实施方案中，所述氨基酸插入、取代和/或缺失的数目为1-30个，优选为1-20个，更优选为1-10个，其中获得的功能变体基本上保持未改变的黄嘌呤酰胺水解酶活性。

在第一方面的一些实施方案中，所述功能变体与SEQ ID NO:1所示的氨基酸序列相差约1、2、3、4、5、6、7、8、9、或10个氨基酸的插入、取代和/或缺失。

在第一方面的一些实施方案中，所述功能变体与SEQ ID NO:1所示的氨基酸序列相差约1、2、3、4或5个氨基酸的插入、取代和/或缺失。

在第一方面的一些实施方案中，所述多肽为分离的多肽。

第二方面，本申请提供了核酸分子，其编码第一方面所述的多肽。

在第二方面的一些实施方案中，本申请的核酸分子包含与编码SEQ ID NO:1和SEQID NO:4-104中任一项所示多肽的核苷酸序列在严格条件下杂交的核苷酸序列，或者由与编码SEQ ID NO:1和SEQ ID NO:4-104中任一项多肽的核苷酸序列特异性杂交且编码与SEQID NO:1和SEQ ID NO:4-104中任一项所示多肽在功能上等同的多肽的核酸序列组成。

本领域技术人员可以常规选择DNA杂交的严格条件。通常较长的探针需要较高的温度，以便进行适当的退火，而较短的探针需要较低的温度。杂交通常取决于当互补链处于低于其解链温度的环境时变性DNA的重退火能力。探针与可杂交序列之间同源性程度越高，所能采用的相对温度越高。于是，较高的相对温度往往使反应条件更严格，而在较低的温度下，则严格度较低。关于杂交反应严格条件的详细描述，可参阅Ausubel等人，CurrentProtocols in Molecular Biology,Wiley Interscience Publishers,(1995)。

在第二方面的一些实施方案中，DNA杂交采用的严格条件包括：1)洗涤时采用低离子强度和高温，例如50℃下的0.015M氯化钠/0.0015M柠檬酸钠/0.1％十二烷基硫酸钠；2)杂交时采用甲酰胺等变性剂，例如42℃下50％(v/v)甲酰胺加0.1％牛血清白蛋白/0.1％Ficoll/0.1％聚二烯吡咯烷酮/pH 6.5的50mM磷酸钠缓冲液以及750mM氯化钠、75mM柠檬酸钠；或(3)在42℃下过夜杂交，杂交溶液含50％甲酰胺，5×SSC(0.75M氯化钠，0.075M柠檬酸钠)、50mM磷酸钠(pH 6.8)、0.1％焦磷酸钠、5×Denhardt’s溶液、超声波处理的鲑鱼精子DNA(50mg/mL)、0.1％SDS和10％硫酸葡聚糖，然后在0.2×SSC(氯化钠/柠檬酸钠)中于42℃洗涤10分钟，再以含有EDTA的0.1×SSC于55℃进行高严格度洗涤。中等严格条件可按Sambrook等人，Molecular Cloning：A Laboratory Manual,NewYork：Cold Spring HarborPress,1989中的描述加以确定。中等严格条件包括采用严格度低于以上描述的洗涤溶液和杂交条件(如温度、离子强度和SDS百分比)。例如，中等严格条件包括用至少约16％v/v到至少约30％v/v的甲酰胺和至少约0.5M到至少约0.9M的盐在42℃杂交，以及用至少约0.1M到至少约0.2M盐在55℃洗涤。中等严格条件还可以包括用1％牛血清白蛋白(BSA)、1mM EDTA、0.5M NaHPO₄(pH 7.2)、7％SDS在65℃杂交，以及用(i)2×SSC、0.1％SDS；或(ii)0.5％BSA、1mM EDTA、40mM NaHPO₄(pH 7.2)、5％SDS在60-65℃洗涤。专业人员将会根据探针长度等因素调节温度、离子强度等。杂交核酸时的严格度取决于核酸分子长度和互补程度，以及其它本领域内众所周知的变量。两个核苷酸序列之间的相似性或同源性越大，则含有这些序列的核酸杂合体的Tm越大。核酸杂交的相对稳定性(对应于较高的Tm)以下列顺序递减：RNA:RNA、DNA:RNA,DNA:DNA。优选地，可杂交核酸的最低长度至少约为12个核苷酸，优选至少约为16个、更优选至少约为24个、最优选至少约为36个核苷酸。

本申请的核酸分子可以与其他DNA序列组合，所述其他DNA序列例如启动子、聚腺苷化信号、其他限制性酶切位点、多克隆位点、其他编码节段等，使得它们的总长度可以显著不同。因此考虑可以利用几乎任意长度的多核苷酸片段；总长度优选地受预期重组DNA方案中制备和使用的便利性的限制。

可以利用本领域内已知的和可获得的多种成熟技术中的任何一种来制备、操控和/或表达多核苷酸及其融合物。例如，编码本申请的多肽或其功能变体的核酸分子可以用于重组DNA分子中以指导多肽在适当的宿主细胞中表达。由于遗传密码子固有的简并性，编码基本上相同或在功能上等同的氨基酸序列的其他DNA序列也可以用于本申请中，并且这些序列可以用于克隆和表达给定的多肽。

此外，可以使用本领域内公知的方法改造本申请的核酸分子，包括但不限于基因产物的克隆、加工、表达和/或活性的改变。

在第二方面的一些实施方案中，所述核酸分子通过人工合成产生，例如直接的化学合成或酶合成。

在第二方面的一些实施方案中，所述核酸分子通过重组技术产生。

在第二方面的一些实施方案中，所述核酸分子为分离的核酸分子。

第三方面，本申请提供了表达盒，其包含第二方面所述的核酸分子。

在第三方面的一些具体实施方案中，表达盒可以另外包含5'前导序列，所述前导序列能发挥增强翻译的作用。

在制备表达盒时，可以操作各种DNA片段以提供合适方向的和适当时合适读框的DNA序列。为达到这一目的，可以采用接头或连接子来连接DNA片段，或者可以涉及其它操作来提供方便的限制性位点、移除多余DNA、移除限制性位点等等。为了这一目的，可以涉及体外诱变，引物修复，限制性，退火，再取代，例如转换(transition)和颠换(transversion)。

第四方面，本申请提供了表达载体，其包含第二方面所述的核酸分子或第三方面所述的表达盒。

在第四方面的一些实施方案中，任何合适的表达载体都可以用于本申请。例如，所述表达载体可以为pET28、pET14、HT-1N-TAG-2691、pRS416等载体中的任一种。

在第四方面的一些实施方案中，编码SEQ ID NO:1和SEQ ID NO:4-104中任一项所示多肽的核酸分子被克隆到载体中，以构成含有本申请所述核酸分子的重组载体。

在第四方面的一些实施方案中，用于克隆多核苷酸的表达载体为质粒载体。

在第四方面的一些的实施方案中，上述表达载体还包含调节核酸分子表达的调控序列，其中所述核酸分子与所述调控序列可操作地连接。

如本文所用的术语“调控序列”是指实现与其连接的编码序列表达所需的多核苷酸序列。这类调控序列的性质随宿主生物而改变。在原核生物中，这类调控序列一般包括启动子、核糖体结合位点和终止子；在真核生物中，这类调控序列一般包括启动子、终止子以及在某些情况下的增强子。因此，术语“调控序列”包括其存在对目的基因的表达是必需的最低限度的所有序列，也可以包括其存在对目的基因表达是有利的其它序列，例如前导序列。

如本文所用的术语“可操作地连接”是指如下情形：所涉及到的序列处于允许它们以希望的方式起作用的关系之中。因此，例如“可操作地连接”到一编码序列的调控序列使得在与所述调控序列相容的条件下实现该编码序列的表达。

在第四方面的一些实施方案中，利用本领域的技术人员熟知的方法构建包含编码SEQ ID NO:1和SEQ ID NO:4-104中任一项所示多肽的核苷酸序列和合适的转录/翻译调控元件的表达载体。这些方法包括体外重组DNA技术、DNA合成技术、体内重组技术等(Sambroook,et al.Molecular Cloning,a Laboratory Manual,cold Spring HarborLaboratory.New York,1989)。核苷酸序列可操作地连接到表达载体中的适当启动子上，以指导mRNA合成。这些启动子的代表性实例包括：大肠杆菌的lac或trp启动子；λ噬菌体的PL启动子；真核启动子包括CMV立即早期启动子、HSV胸苷激酶启动子、早期和晚期SV40启动子、反转录病毒的LTRs和其它一些已知的可控制基因在原核细胞或真核细胞或其病毒中表达的启动子。表达载体还包括翻译起始用的核糖体结合位点和转录终止子等。在载体中插入增强子序列将会使其在高等真核细胞中的转录得到增强。增强子是DNA表达的顺式作用因子，通常大约有10到300个碱基对，作用于启动子以增强基因的转录。实例包括在复制起始点晚期一侧的100到270个碱基对的SV40增强子、在复制起始点晚期一侧的多瘤增强子以及腺病毒增强子等。

此外，表达载体优选地包含一个或多个选择性标记基因，以提供用于选择转化的宿主细胞的表型性状，如编码抗硫酸卡那霉素、抗氨苄青霉素等的那些基因。

第五方面，本申请提供了宿主细胞，其包含第二方面所述的核酸分子或第三方面所述的表达盒或第四方面所述的表达载体。

在第五方面的一些实施方案中，所述宿主细胞能够表达并产生：多肽，其包含SEQID NO:1所示的氨基酸序列或其功能变体，其中所述功能变体具有黄嘌呤酰胺水解酶活性。

在第五方面的一些实施方案中，其中所述多肽在空间构象上具有如下定义的催化部位：

所述催化部位包含空间构象上相互靠近的、参照SEQ ID NO:1的H59、H61、K151、H186、H242和D316氨基酸残基。

在第五方面的一些实施方案中，所述催化部位还包含二价金属离子。

在第五方面的一些具体实施方案中，所述催化部位还包含2个二价金属离子，例如Zn²⁺和/或Mn²⁺。

在第五方面的一些具体实施方案中，其中所述多肽在空间构象上具有如下定义的催化部位和结合部位：

所述催化部位包含空间构象上相互靠近的、参照SEQ ID NO:1的H59、H61、K151、H186、H242和D316氨基酸残基以及2个二价金属离子(例如Zn²⁺和/或Mn²⁺)；和

所述结合部位包含在空间构象上相互靠近的、参照SEQ ID NO:1的I288、A289、P338和G339氨基酸残基。

在第五方面的一些实施方案中，可用的宿主细胞为含有上述表达载体的细胞，可以为真核细胞，例如酵母细胞培养系统可用于本申请的多肽的表达。所述宿主细胞也可以为含有上述表达载体的原核细胞，例如可以选自埃希氏菌属(例如大肠杆菌)、乳酸杆菌属、双歧杆菌属、拟杆菌门和厚壁菌门等。

在第五方面的一些具体实施方案中，所述宿主细胞为酵母细胞或大肠杆菌。

在第五方面的一些具体实施方案中，编码本申请的一种或多种酶的核酸分子可以以游离载体的形式存在于宿主细胞中，或者也可以整合至宿主细胞的基因组中。

在上述任一方面的一些实施方案中，所述分离的核酸可操作地连接到调控序列，调控序列可以被用所述表达载体转化过的宿主细胞识别。

可以利用本领域已知的任何技术将表达载体导入宿主细胞中，包括转化、转导、转染、病毒感染、基因枪或Ti-介导的基因转移。具体的方法包括磷酸钙转染、DEAE-葡聚糖介导的转染、脂转染或电穿孔等(Davis,L.,Dibner,M.,Battey,I.,Basic Methods inMolecular Biology,(1986))。作为示例，当宿主为原核生物如大肠杆菌时，可在指数生长期后收获感受态细胞，用本领域熟知的CaCl₂法进行转化。

第六方面，本申请提供了药物组合物或保健食品，其包含第一方面所述的多肽、第二方面所述的核酸分子、第三方面所述的表达盒、第四方面所述的表达载体或第五方面所述的宿主细胞和药学上可接受的载体或赋形剂。

在第六方面的一些实施方案中，所述药物组合物或保健食品，用于预防、干预和/或治疗痛风。

在第六方面的一些实施方案中，所述药物组合物或保健食品还可包含下述中的一种或多种：润滑剂，如滑石粉、硬脂酸镁和矿物油；润湿剂；乳化剂；悬浮剂；防腐剂，如苯甲酸、山梨酸和丙酸钙；增甜剂和/或调味剂等。

在第六方面的一些实施方案中，可将本申请中的药物组合物或保健食品配制为片剂、丸剂、粉剂、锭剂、酏剂、悬液、乳剂、溶液、糖浆、栓剂或胶囊等形式。

在第六方面的一些实施方案中，可以利用任何能给药至肠道的方式递送本申请的药物组合物或保健食品，优选口服给药。

在第六方面的一些具体实施方案中，将第五发明所述的宿主细胞制备成肠溶胶囊用于口服给药。

在第六方面的一些实施方案中，可以通过混合具有所需纯度的试剂与视情况的药学上可接受的载体、赋形剂等，以冻干制剂或水溶液的形式配制用于治疗用途的药物组合物用于存储。

第七方面，本申请提供了第一方面所述的多肽、第二方面所述的核酸分子、第三方面所述的表达盒、第四方面所述的表达载体、第五方面所述的宿主细胞或第六方面所述的药物组合物或保健食品在降解嘌呤中的用途。

在第七方面的一些实施方案中，所述降解嘌呤在体外发生。

在第七方面的一些实施方案中，降解嘌呤在无氧的环境下进行，例如肠道中。

第八方面，本申请提供了第一方面所述的多肽、第二方面所述的核酸分子、第三方面所述的表达盒、第四方面所述的表达载体、第五方面所述的宿主细胞或第六方面所述的药物组合物或保健食品在制备用于预防、干预和/或治疗痛风的药物中的用途。

第九方面，本申请提供了预防、干预和/或治疗痛风的方法，其包括向有需要的个体给予第一方面所述的多肽、第二方面所述的核酸分子、第三方面所述的表达盒、第四方面所述的表达载体、第五方面所述的宿主细胞或第六方面所述的药物组合物或保健食品。

作为示例性的实施方案，在黄嘌呤的无氧降解中，SEQ ID NO:1所示的黄嘌呤酰胺水解酶将黄嘌呤降解为4-脲基-5-羧基咪唑，从而减少人体细胞获得嘌呤并将其转化为尿酸，可以通过此途径在肠道内降解黄嘌呤从而预防、干预和/或治疗痛风。

实施例

下述实施例仅是说明性的，并不意图限制本申请实施方案的范围或者所附的权利要求的范围。

实施例1.编码黄嘌呤酰胺水解酶的基因克隆的制备

以坚强芽孢杆菌(Bacillus firmus)(CGMCC 1.2010)的基因组作为模板，使用SEQID NO：2和3所示的上游引物和下游引物PCR扩增编码黄嘌呤酰胺水解酶(SEQ ID NO:1)的核酸分子，获得编码黄嘌呤酰胺水解酶的基因序列。利用Gibson的方法将编码黄嘌呤酰胺水解酶的基因序列插入HT-1N-TAG-2691载体(其含有His₆标签和TEV蛋白酶切割位点)的SspI限制性位点，获得含有编码黄嘌呤酰胺水解酶的基因的表达载体。

实施例2.黄嘌呤酰胺水解酶的重组表达和纯化

2.1重组表达

将实施例1中构建的含有编码黄嘌呤酰胺水解酶的基因1的表达载体转化到大肠杆菌(Escherichia coli)BL21细胞中，涂布在含有50μg/mL硫酸卡那霉素的LB琼脂平板上37℃过夜培养。挑取单克隆菌落在5mL的含硫酸卡那霉素LB液体培养基中37℃、220rpm过夜培养，次日转移到800mL的LB培养液中扩大培养，当OD₆₀₀值达到0.8左右时,将温度降到18℃，并加入终浓度为0.3mM的IPTG诱导蛋白表达。诱导表达16小时后在4℃下4000×g离心10min收集菌体。将菌体沉淀用35mL裂解液重悬[50mM Tris/HCl,pH 8.0，1mM苯甲基磺酰氟(PMSF)，0.2mg/mL溶菌酶，0.03％Triton X-100和1μL的DNase I]，并在-80℃下冷冻保藏。

2.2纯化

将菌体从-80℃中取出，置于25℃水浴中孵育40分钟以裂解细胞。加入6mL的6％硫酸链霉素水溶液，温和混匀，在4℃下以20,000×g转速离心5分钟。将上清用0.22μm的滤膜过滤，结合到预先用缓冲液A(20mM Tris-HCl 7.5，0.2M KCl)平衡过的5mL TALON钴柱上，用10倍柱体积的缓冲液A洗涤柱子，然后用5倍柱体积的含有150mM咪唑的缓冲液B(20mMTris-HCl 7.5，0.2M KCl，150mM咪唑，5mMβ-巯基乙醇)洗脱目的蛋白。向洗脱下来的蛋白溶液中加入硫酸铵至70％饱和沉淀蛋白，在10,000×g下离心10分钟，弃去上清，沉淀用5mL缓冲液C(20mM Tris-HCl 7.5，0.2M KCl，10％(v/v)丙三醇)重悬，使用G25柱，除去盐。将除去盐后的目的蛋白分装，用液氮速冻，置于-80℃中冷冻保存。通过分光光度计检测最终所得到的黄嘌呤酰胺水解酶的浓度。根据蛋白序列，黄嘌呤酰胺水解酶的消光系数是55,810M^{- 1}cm^-1。每升菌平均大约能纯化35mg蛋白。图5显示黄嘌呤酰胺水解酶表达、纯化的过程，其在凝胶电泳上的迁移速率符合实际的分子量大小(55.7kDa)。

实施例3.黄嘌呤酰胺水解酶的活性检测

将1mL含有1mM黄嘌呤、4μM实施例2中获得的黄嘌呤酰胺水解酶、0.1mM Mn²⁺、200mMTris-HCl 7.5的溶液在30℃水浴中孵育0.5h，使体系充分反应，然后加入等体积的甲醇溶液混匀，并在14,000×g下离心10分钟，通过0.22μm滤膜过滤掉蛋白沉淀，记为实验组。对照组为未添加黄嘌呤酰胺水解酶及二价金属离子的反应，对照组与实验组的其它实验条件相同(表1)。实验在无氧手套箱内进行，所有溶液包括蛋白溶液，反应缓冲液等在进入手套箱前均通过Schlenk(希莱克)线除氧。

利用LC-MS检测黄嘌呤酰胺水解酶催化黄嘌呤水解开环，对照组未检测到产物峰，而实验组检测到了分子量为169Da的产物峰，证明了黄嘌呤酰胺水解酶可以降解黄嘌呤(图6)。

表1

实施例4.黄嘌呤酰胺水解酶的活性中心鉴定

在体外证实含有SEQ ID NO:1所示的氨基酸序列的多肽具有黄嘌呤酰胺水解酶活性后，利用PHYRE2服务器对该酶进行结构模拟(图7)，显示出了含有4个组氨酸、一个赖氨酸和一个天冬氨酸以及两个二价金属离子(Zn²⁺和/或Mn²⁺)构成的催化部位。进一步用计算机软件对模拟的黄嘌呤酰胺水解酶进行底物黄嘌呤的锚定显示，活性中心还包括由一个异亮氨酸、一个丙氨酸、一个脯氨酸和一个甘氨酸在空间构象上相互靠近构成的结合部位与底物黄嘌呤相互作用(图7)。

实施例5.黄嘌呤酰胺水解酶的同工酶鉴定

本申请的发明人进一步从Uniprot数据库中找到此黄嘌呤酰胺水解酶同工酶UniRef50的序列(涵盖50％序列相同，同时超过该黄嘌呤酰胺水解酶80％长度的蛋白序列)，上述序列均在UniRef50_Q3AEA1的簇中，共包括101种蛋白序列，并对这101种不同物种来源的蛋白进行进化树分析(图8)。在每一个进化分支中各选取一个代表性蛋白，共五种蛋白，分别来源于坚强芽孢杆菌(Bacillus firmus)、柱胞梭菌DSM 605(Clostridiumcylindrosporum DSM 605)、Clostridium purinilyticum、产氢羧基嗜热菌(Carbydothermus hydrogenoformans，菌株ATCC BAA-161/DSM 6008/Z-2901)以及Paenibacillus donghaensis，将它们编码的登录号分别为A0A366K523、A0A0J8G334、A0A0L0W692、Q3AEA1和A0A2Z2KEH1的黄嘌呤酰胺水解酶的氨基酸序列进行序列比对。序列对比结果显示上述催化位点和结合位点的氨基酸残基高度保守，所有五个序列都含有上述氨基酸残基，证明这些酶均为黄嘌呤酰胺水解酶(图9)。表2显示了从Uniprot数据库中找到的101种黄嘌呤酰胺水解酶的登录号、菌株来源以及氨基酸序列号。

表2

实施例6.表达黄嘌呤酰胺水解酶的大肠杆菌的构建

本实施例构建了能表达黄嘌呤酰胺水解酶(SEQ ID NO:1)的大肠杆菌。

本实施例的关键操作是把编码黄嘌呤酰胺水解酶的基因整合到大肠杆菌基因组，例如多拷贝16sRNA其中一个拷贝处，让其稳定表达，同时把控制鸟嘌呤转运蛋白和鸟嘌呤脱氨酶的启动子置换为gapA启动子，让编码黄嘌呤酰胺水解酶的基因持续稳定表达。

本实施例优选用组成型持续稳定表达的启动子gapA取代编码鸟嘌呤转运蛋白以及鸟嘌呤脱氨酶的启动子，原启动子通常在环境中氮源缺少，需要利用鸟嘌呤的氮的情况下才表达，在氮源充足的情况下，其控制的基因不表达，为了使食物中的鸟嘌呤能通过本发明的黄嘌呤酰胺水解酶降解，需要取代控制编码鸟嘌呤转运蛋白和鸟嘌呤脱氨酶的基因的启动子，使其在任何时候都获得表达，从而有效的把鸟嘌呤转运进入经上述工程改造的大肠杆菌细胞内，并通过鸟嘌呤脱氨酶和黄嘌呤酰胺水解酶顺序作用降解，从而有效阻止人体肠道对鸟嘌呤的吸收并将其转化成尿酸。

实施例7.表达黄嘌呤酰胺水解酶的大肠杆菌用于治疗痛风

将利用氧嗪酸(oxonic acid)诱导的高尿酸大鼠或尿酸酶敲除转基因小鼠作为痛风动物模型，将实施例6制备的各种大肠杆菌制成肠溶胶囊。在同等喂食的条件下，检测各种大肠杆菌肠溶胶囊能否降低痛风大鼠、小鼠模型的血液尿酸含量。

上文对本申请的各项发明的示例性实施方案进行了描述，但是，在不脱离本申请的实质和范围的情况下，本领域技术人员能够对本申请描述的示例性实施方案进行修改或改进，由此得到的变形方案或等同方案也属于本申请的范围。

序列表

<110> 天津大学

<120> 黄嘌呤酰胺水解酶及其用途

<160> 104

<170> SIPOSequenceListing 1.0

<210> 1

<211> 485

<212> PRT

<213> 坚强芽孢杆菌(Bacillus firmus)

<400> 1

Asp Leu Ile Leu Lys Asn Ala Ser Ile Pro Gln Gly Asp Arg Gln Val

1 5 10 15

Leu Thr Asn Ile Leu Val Asn Glu Gly Lys Ile Val Gly Tyr Thr Asn

20 25 30

Asp Ile Ser Phe Leu Asn Ala Ala Arg Val Ile Asp Ile Lys Gly Lys

35 40 45

Leu Val Val Pro Gly Cys Ile Asp Pro His Thr His Phe Met Asp Pro

50 55 60

Gly Phe Thr His Arg Glu Thr Phe Ala Thr Gly Ser Arg Ser Ala Ala

65 70 75 80

Ala Gly Gly Leu Thr Thr Ile Ile Asp Met Pro Cys Cys Ser Lys Pro

85 90 95

Ser Val Arg Asp Gly Glu Ser Phe Asn Lys Lys Ile Gly Pro Ile Arg

100 105 110

Asp Gln Ala Tyr Ile Asp Tyr Cys Phe Trp Gly Gly Met Thr Gly Glu

115 120 125

Asp Ala Arg Glu Gly Trp Ile Asp Asn Ile Tyr Pro Gln Ile Asp Gln

130 135 140

Gly Val Val Ala Phe Lys Val Tyr Met Thr Pro Ser Val Pro Thr Phe

145 150 155 160

Pro Lys Val Ser Asp Ala Glu Met Leu Glu Ile Phe Ser Asn Val Ala

165 170 175

Lys Thr Gly Leu Pro Ile Gly Val His Ala Glu Asn Tyr Asp Ile Cys

180 185 190

Thr Phe Tyr Ser Lys Lys Leu Glu Lys Glu Gly Arg Leu Asp Gly Pro

195 200 205

Ala Trp Ala Glu Ala Arg Ser Ser Leu Ala Glu Lys Val Ala Ile Glu

210 215 220

Leu Ile Leu Ser Phe Ala Glu Ala Thr Asp Ala Arg Val His Val Val

225 230 235 240

His Met Ser Thr Lys Glu Gly Val Glu Leu Val Lys Ala Ala Lys Lys

245 250 255

Arg Gly Val Lys Val Thr Ala Glu Thr Cys Pro His Tyr Leu Val Leu

260 265 270

Asn Ala Lys Asp Ser Met Ser Glu Arg Gly Ser Tyr Ala Lys Ile Ala

275 280 285

Pro Pro Leu Arg Gly Lys Glu Asp Asn Glu Ser His Trp Gln Ala Leu

290 295 300

Ala Asp Gly Thr Ile Asp Phe Val Gly Thr Asp His Ala Pro Tyr Glu

305 310 315 320

Ile Ala Thr Glu Lys Asp Tyr Pro Gly Ala Thr Ile Trp Asn Thr Phe

325 330 335

Pro Gly Ile Pro Gly Val Glu Thr Met Val Pro Ile Leu Val Ser Glu

340 345 350

Gly Leu Asn Lys Gly Arg Leu Ser Leu Ser Arg Phe Val Glu Val Thr

355 360 365

Ser Arg Asn Ala Ala Ile His Tyr Gly Ile Tyr Pro Lys Lys Gly Ser

370 375 380

Met Glu Ile Gly Ser Asp Ala Asp Phe Thr Val Ile Asp Leu Glu Lys

385 390 395 400

Glu Tyr Val Ile Asp Glu Gln Lys Thr Glu Ser Met Ala Lys Tyr Asn

405 410 415

Pro Leu His Gly Met Lys Leu Lys Gly Lys Pro Val Gln Thr Ile Ile

420 425 430

Arg Gly Asn Val Ile Phe Asp Glu Asp Lys Gly Ile Val Gly Glu Ala

435 440 445

Gly Tyr Gly Glu Tyr Val Pro Arg Gln Ser Ile Gln Arg Leu Glu Arg

450 455 460

Thr Phe Lys Tyr Glu Lys Tyr Glu Ala Val Lys Ala Lys Thr Lys Asp

465 470 475 480

Pro Val Ala Gln Gln

485

<210> 2

<211> 44

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 2

cctgtacttc caatccaatg atttaatttt gaaaaatgca agca 44

<210> 3

<211> 37

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 3

ccgttatcca cttccaattt attgctgtgc tactggg 37

<210> 4

<211> 531

<212> PRT

<213> Clostridium purinilytica

<400> 4

Met Tyr Asp Leu Ile Leu Lys Asn Ala Arg Ile Pro Gln Gly Asp Asp

1 5 10 15

Thr Ile Val Thr Asn Ile Leu Val Arg Asp Glu Lys Ile Ala Gly Phe

20 25 30

Thr Asp Cys Ile Glu Glu Val Gln Ala Lys Glu Ile Ile Asp Ile Glu

35 40 45

Gly Gln Leu Thr Leu Pro Gly Cys Ile Asp Ser His Thr His Phe Met

50 55 60

Tyr Gln Gly Phe Pro His Arg Glu Asn Phe Leu Thr Gly Thr Ala Ala

65 70 75 80

Ala Ala Thr Gly Gly Ile Thr Thr Val Ile Asp Met Pro Cys Cys Ser

85 90 95

Val Pro Ser Ala Arg Ser Val Glu Gln Leu Gln Leu Lys Ile Asp Leu

100 105 110

Cys Gln Pro Gln Ser Leu Val Asp Phe Ala Met Trp Gly Gly Val Thr

115 120 125

Gly Glu Asp Val Arg Glu Asp Trp Met His Asn Val Lys Glu Gln Ala

130 135 140

Asp Tyr Gly Val Val Ala Phe Lys Val Tyr Met Thr Pro Ser Val Pro

145 150 155 160

Thr Tyr Pro Arg Val Thr Asp Pro Glu Met Leu Glu Cys Phe Arg Ala

165 170 175

Val Ala Glu Thr Gly Leu Pro Ile Gly Ile His Ala Glu Asn Phe Ala

180 185 190

Met Cys Asp Phe Tyr Val Lys Lys Phe Gln Lys Glu Gly Arg Met Asp

195 200 205

Gly Pro Ala Trp Ala Glu Ala Arg Met Glu Leu Ala Glu Lys Val Ala

210 215 220

Ile Glu Leu Gly Ile Ser Phe Ala Glu Asp Thr Gly Ala Arg Leu His

225 230 235 240

Ile Val His Met Ser Thr Gly Ile Gly Ala Lys Leu Val Gly Glu Ala

245 250 255

Lys Lys Arg Gly Ile Asn Val Thr Ser Glu Thr Cys Pro His Tyr Leu

260 265 270

Thr Leu Asn Tyr Gln Asp Ala Met Ser Glu Tyr Gly Ala Phe Ala Lys

275 280 285

Ile Ala Pro Pro Leu Arg Thr Lys Arg Asp Asn Glu Glu Leu Trp Glu

290 295 300

Gly Leu Asn Asn Gly Ser Val Asp Phe Ile Ala Thr Asp His Ala Pro

305 310 315 320

Tyr Glu Ile Glu Ser Glu Lys Ala Lys Glu Gly Met Asn Ile Trp Thr

325 330 335

Ala Phe Pro Gly Ile Pro Gly Val Glu Thr Met Val Pro Val Leu Val

340 345 350

Ser Glu Gly Tyr Asn Lys Gly Arg Leu Ser Leu Ser Lys Leu Val Asp

355 360 365

Ile Leu Ser Thr Asn Ala Ala Lys His Tyr Gly Leu Tyr Pro Lys Lys

370 375 380

Gly Ala Leu Asn Ile Gly Ser Asp Ala Asp Phe Thr Val Ile Asp Leu

385 390 395 400

Asp Arg Glu Trp Thr Ile Thr Lys Asp Met Val Thr Met Cys Gly Tyr

405 410 415

Asn Pro Leu Glu Gly Leu Lys Leu Lys Gly Lys Pro Val Lys Thr Ile

420 425 430

Val Arg Gly His Ile Val Tyr Gln Asp Gly Glu Glu Gly Ala Leu Pro

435 440 445

Ser Leu Thr Asp Glu Glu Leu Lys Thr Ile Val His Glu Tyr Pro Ala

450 455 460

Gly Val Glu Glu Lys Tyr Ser Gly Val Phe Glu Gly Phe Pro His Leu

465 470 475 480

His Ser Ala Glu Tyr Glu Arg Ser Tyr Arg Lys Glu His Pro Glu Val

485 490 495

Ile Asp Glu His Ile Arg Gly Ile Lys Gly Ile Met Val Lys Pro Gly

500 505 510

Phe Gly Gln Phe Val Lys Arg Gln Ser Ile Gln Val Leu Pro Lys Thr

515 520 525

Ile Thr Tyr

530

<210> 5

<211> 472

<212> PRT

<213> 高温黄色微球菌(Thermoflavimicrobium sp. )

<400> 5

Met Gln Leu Ile Leu Lys Asn Ala Arg Ile Pro Gln Gly Gln Gly Asn

1 5 10 15

Asp Thr Val Ile Thr Asn Ile Leu Val Asn Glu Asn Lys Ile Ala Gly

20 25 30

Phe Val Asn His Val Asp Gly Ile Glu Ala Gln Gln Val Ile Asp Leu

35 40 45

Glu Gly Lys Leu Val Val Pro Gly Cys Ile Asp Pro His Thr His Phe

50 55 60

Met Asp Pro Gly Phe Thr His Arg Glu Thr Phe Glu Thr Gly Ser Ala

65 70 75 80

Ser Ala Ala Ala Gly Gly Leu Thr Thr Ile Val Asp Met Pro Cys Cys

85 90 95

Ser Lys Pro Ser Val Arg Ser Lys Asp Glu Phe His Ala Lys Trp Asp

100 105 110

Lys Val Lys Asn Asn Ser Tyr Ile Asp Phe Gly Ser Trp Gly Gly Met

115 120 125

Thr Gly Glu Asp Val Arg Glu Asp Lys Leu Asp Asn Val Trp Gly Gln

130 135 140

Val Glu Glu Gly Ile Thr Cys Phe Lys Val Tyr Met Thr Pro Ser Val

145 150 155 160

Pro Thr Phe Pro Lys Val Thr Asp Ala Glu Met Leu Glu Ile Phe Ala

165 170 175

Lys Val Ala Glu Thr Gly Leu Pro Val Gly Ile His Ala Glu Asn Phe

180 185 190

Asp Ile Cys Asp Phe Arg Val Lys Lys Leu Arg Lys Glu Gly Arg Met

195 200 205

Asp Ala Pro Ala Trp Ser Glu Ala Arg Asn Asn Leu Ala Glu Lys Val

210 215 220

Ala Ile Glu Leu Val Ile Ser Phe Ala Glu Ala Thr Gly Ala Arg Ala

225 230 235 240

His Ile Val His Met Ser Thr Lys Glu Gly Val Glu Leu Val Arg Gln

245 250 255

Ala Lys Leu Arg Gly Val Pro Ile Thr Ala Glu Thr Cys Pro His Tyr

260 265 270

Leu Val Leu Asn Tyr Lys Asp Ala Leu Thr Glu Phe Gly Thr Phe Ala

275 280 285

Lys Val Ala Pro Pro Leu Arg Glu Thr Ala Asp Asn Glu Ala His Trp

290 295 300

Lys Ala Leu Ala Asp Gly Thr Ile Asp Phe Val Ala Thr Asp His Ala

305 310 315 320

Pro Tyr Glu Ile Pro Ile Glu Lys Val Gly Glu Gly Leu Asn Ile Trp

325 330 335

Asp Ser Phe Pro Gly Phe Pro Gly Val Glu Thr Met Val Pro Val Leu

340 345 350

Val Ser Glu Gly Leu Asn Lys Gly Arg Leu Thr Leu Gln Arg Phe Val

355 360 365

Glu Val Thr Ser Thr Asn Gly Ala Ile Leu His Gly Leu Tyr Pro Gln

370 375 380

Lys Gly His Met Gly Met Gly Ala Asp Ala Asp Phe Thr Val Ile Asp

385 390 395 400

Leu Glu Arg Glu Tyr Thr Ile Asp Lys Asp Lys Val His Thr Met Ala

405 410 415

Lys Tyr Thr Pro Phe His Gly Met Lys Leu Lys Gly Lys Pro Ile Met

420 425 430

Thr Ile Val Arg Gly Thr Val Val Tyr Asp Glu Asp Arg Gly Gly Leu

435 440 445

Val Gly Gln Val Gly Tyr Gly Gln Tyr Val Arg Arg Gln Thr Asn Ile

450 455 460

His Pro Pro Arg Gln Ile Gln Phe

465 470

<210> 6

<211> 473

<212> PRT

<213> 耐盐海洋丝状菌(Marininema halotolerans)

<400> 6

Met Asp Leu Leu Leu Lys Asn Ala Asn Ile Pro Gln Gly Asp Arg Gln

1 5 10 15

Val Gln Thr Asn Ile Leu Val Asp Glu Gly Lys Ile Val Gly Phe Val

20 25 30

Gln Asp Ala Ser Gly Val Met Ala Glu Arg Thr Ile Asp Leu Thr Gly

35 40 45

Lys Leu Val Val Pro Gly Cys Ile Asp Pro His Thr His Phe Met Asp

50 55 60

Pro Gly Phe Thr His Arg Glu Thr Phe Ala Glu Ala Ser Met Ser Ala

65 70 75 80

Ala Ala Gly Gly Leu Thr Cys His Val Asp Met Pro Cys Cys Ser Lys

85 90 95

Pro Ser Val Arg Ser Glu Asp Glu Leu Tyr Met Lys Leu Asn Pro Ile

100 105 110

Lys Asp Gln Ala Tyr Ile Asp Phe Ala Phe Trp Gly Gly Met Thr Gly

115 120 125

Glu Asp Val Arg Glu Gly Lys Leu Asp Asn Val Trp Gly Gln Val Glu

130 135 140

Ala Gly Val Thr Ala Phe Lys Val Tyr Met Thr Pro Ser Val Pro Thr

145 150 155 160

Phe Pro Lys Val Ser Asp Ala Glu Met Leu Glu Ile Phe Gln Lys Val

165 170 175

Ala Glu Thr Gly Leu Pro Val Gly Val His Ala Glu Asn Tyr Asp Ile

180 185 190

Cys Ser Phe Tyr Ala Asn Lys Leu Glu Lys Glu Gly Arg Thr Asp Ser

195 200 205

Pro Ala Trp Ala Glu Ala Arg Met Ala Leu Ala Glu Lys Ala Ala Ile

210 215 220

Gln Leu Ile Ile Ser Phe Ala Glu Glu Thr Gly Ala Arg Ala His Ile

225 230 235 240

Val His Met Ser Thr Lys Glu Gly Ala Ala Leu Ile Arg Asp Ala Lys

245 250 255

Arg Arg Gly Val Lys Ile Thr Ala Glu Ala Cys Pro His Tyr Leu Thr

260 265 270

Leu Asn Ala Lys Glu His Met Thr Gln Phe Gly Asn Leu Ala Lys Ile

275 280 285

Ala Pro Pro Leu Arg Gly Lys Glu Asp Asn Glu Val His Trp Gln Ala

290 295 300

Leu Ser Asp Gly Thr Ile Asp Phe Val Gly Thr Asp His Ala Pro Tyr

305 310 315 320

Glu Ile Ala Thr Glu Lys Val Ala Asp Gly Leu Thr Ile Trp Asn Ser

325 330 335

Phe Pro Gly Ile Pro Gly Val Glu Thr Met Val Pro Val Met Val Ser

340 345 350

Glu Gly Leu Asn Lys Gly Arg Leu Ser Leu Ser Arg Phe Val Glu Val

355 360 365

Thr Ser Arg Asn Ala Ala Ile His Phe Gly Leu Tyr Pro Lys Lys Gly

370 375 380

Ser Met Glu Leu Gly Ser Asp Ala Asp Phe Thr Val Ile Asp Leu Asp

385 390 395 400

Arg Glu Tyr Val Ile Asp Gln Glu Lys Thr Leu Thr Lys Ala Lys Tyr

405 410 415

Thr Pro Phe His Gly Met Lys Leu Lys Gly Lys Pro Val Met Thr Ile

420 425 430

Val Arg Gly Gly Val Val Tyr Asp Glu Asp His Gly Gly Ile Gln Ala

435 440 445

Thr Ala Gly Tyr Gly Gln Phe Val Lys Arg Gln Ser Asn Ile Glu Leu

450 455 460

Lys Arg Val Glu Gly Ser Leu Ile Tyr

465 470

<210> 7

<211> 470

<212> PRT

<213> 梭菌科细菌(Clostridiaceae bacterium)

<400> 7

Met Ser Ile Asp Leu Leu Ile Lys Asn Ala Met Ile Pro Lys Gly Asp

1 5 10 15

Thr Thr Val Lys Val Asp Ile Leu Val Asp Lys Gly Ile Ile Val Gly

20 25 30

Phe Met Glu Gln Ala Gln Ile Gln Ala Lys Glu Ile Ile Asp Ala Cys

35 40 45

Glu Lys Leu Val Ile Pro Gly Cys Ile Asp Ser His Thr His Ile Asn

50 55 60

Asp Pro Gly Phe Thr His Arg Glu Asn Phe Leu Thr Gly Thr Ser Ala

65 70 75 80

Ala Ala Ser Gly Gly Val Thr Thr Ile Ile Asp Met Pro Cys Cys Ser

85 90 95

Val Pro Ser Val Arg Ser Val Asp Asn Leu Glu Tyr Lys Leu Ser Gln

100 105 110

Ile Ala Asp Lys Ala Ile Val Asp Tyr Gly Met Trp Gly Gly Val Thr

115 120 125

Gly Glu Asp Val Arg Asn Asn Trp Leu His Asn Val Lys Glu Gln Ser

130 135 140

Asp Tyr Gly Val Cys Ala Phe Lys Val Tyr Met Thr Pro Ser Val Pro

145 150 155 160

Thr Tyr Pro Arg Val Thr Asp Pro Glu Met Leu Glu Ala Phe Lys Ser

165 170 175

Val Ala Ala Thr Gly Leu Pro Ile Gly Ile His Ala Glu Asn Tyr Ala

180 185 190

Met Cys Asp Phe Tyr Val Gln Lys Leu Lys Asn Glu Gly Arg Leu Asp

195 200 205

Gly Pro Ala Trp Ala Glu Ala Arg Leu Glu Leu Ala Glu Lys Val Ala

210 215 220

Ile Glu Leu Gly Ile Ser Leu Ala Glu Phe Thr Gly Ala Arg Leu His

225 230 235 240

Ile Val His Met Ser Thr Gly Ile Gly Ala Val Leu Val Lys Asn Ala

245 250 255

Lys Met Arg Gly Leu Asp Val Thr Ser Glu Thr Cys Pro His Tyr Leu

260 265 270

Thr Ile Asn Tyr Gln Asp Ala Met Ser Lys Tyr Glu Ser Phe Ala Lys

275 280 285

Ile Ala Pro Pro Leu Arg Thr Val Lys Asp Asn Glu Met Leu Trp Glu

290 295 300

Gly Val Ala Asp Gly Ser Val Asp Phe Ile Ala Thr Asp His Ala Pro

305 310 315 320

Tyr Glu Ile Ser Thr Glu Lys Cys Ala Glu Gly Met Asn Ile Trp Asn

325 330 335

Ser Phe Pro Gly Ile Pro Gly Val Glu Thr Met Val Pro Val Ile Val

340 345 350

Ser Glu Gly Tyr Asn Lys Gly Lys Ile Ser Leu Ser Arg Met Val Glu

355 360 365

Ile Leu Cys Lys Asn Pro Ala Ile His Tyr Gly Leu Tyr Pro Lys Lys

370 375 380

Gly Ser Met Glu Ile Gly Ser Asp Ala Asp Phe Thr Ile Ile Asp Leu

385 390 395 400

Asp Lys Glu Trp Thr Ile Glu Gln Glu Lys Met Tyr Thr Met Ala Lys

405 410 415

Tyr Thr Pro Leu His Gly Met Thr Leu Lys Gly Lys Pro Val Lys Thr

420 425 430

Ile Val Arg Gly Ser Leu Val Tyr Glu Asp Gly Lys Gly Ile Ile Ala

435 440 445

Lys Pro Gly Tyr Gly Arg Phe Val Lys Arg Gln Thr Ile Ser Lys Leu

450 455 460

Pro Arg Gln Ile Lys Phe

465 470

<210> 8

<211> 486

<212> PRT

<213> 芽孢杆菌(Bacillus sp.)

<400> 8

Met Asp Leu Ile Leu Lys Asn Ala Ser Ile Pro Gln Gly Asp Arg Gln

1 5 10 15

Val Leu Thr Asn Ile Leu Val Asn Glu Gly Lys Ile Val Gly Tyr Thr

20 25 30

Asn Asp Ile Ser Phe Leu Asn Ala Ala Arg Val Ile Asp Ile Lys Arg

35 40 45

Lys Leu Val Val Pro Gly Cys Ile Asp Pro His Thr His Phe Met Asp

50 55 60

Pro Gly Phe Thr His Arg Glu Thr Phe Ala Thr Gly Ser Arg Ser Ala

65 70 75 80

Ala Ala Gly Gly Leu Thr Thr Ile Ile Asp Met Pro Cys Cys Ser Lys

85 90 95

Pro Ser Val Arg Asp Gly Glu Ser Phe Asn Lys Lys Ile Gly Pro Ile

100 105 110

Arg Asp Gln Ala Tyr Ile Asp Tyr Cys Phe Trp Gly Gly Met Thr Gly

115 120 125

Glu Asp Ala Arg Glu Gly Trp Ile Asp Asn Ile Tyr Pro Gln Ile Asp

130 135 140

Gln Gly Val Val Ala Phe Lys Val Tyr Met Thr Pro Ser Val Pro Thr

145 150 155 160

Phe Pro Lys Val Ser Asp Ala Glu Met Leu Glu Ile Phe Arg Asn Val

165 170 175

Ala Lys Thr Gly Leu Pro Ile Gly Val His Ala Glu Asn Tyr Asp Ile

180 185 190

Cys Thr Phe Tyr Ser Lys Lys Leu Glu Lys Glu Gly Arg Leu Asp Gly

195 200 205

Pro Ala Trp Ala Glu Ala Arg Ser Ser Leu Ala Glu Lys Val Ala Ile

210 215 220

Glu Leu Ile Leu Ser Phe Ala Glu Ala Thr Asp Ala Arg Val His Val

225 230 235 240

Val His Met Ser Thr Lys Glu Gly Val Glu Leu Val Lys Ala Ala Lys

245 250 255

Lys Arg Gly Val Lys Val Thr Ala Glu Thr Cys Pro His Tyr Leu Val

260 265 270

Leu Asn Ala Lys Asp Ser Met Ser Glu Arg Gly Ser Tyr Ala Lys Ile

275 280 285

Ala Pro Pro Leu Arg Gly Lys Glu Asp Asn Glu Ser His Trp Gln Ala

290 295 300

Leu Ala Asp Gly Thr Ile Asp Phe Val Gly Thr Asp His Ala Pro Tyr

305 310 315 320

Glu Ile Ala Thr Glu Lys Asp Tyr Pro Gly Ala Thr Ile Trp Asn Thr

325 330 335

Phe Pro Gly Ile Pro Gly Val Glu Thr Met Val Pro Ile Leu Val Ser

340 345 350

Glu Gly Leu Asn Lys Gly Arg Leu Ser Leu Ser Arg Phe Val Glu Val

355 360 365

Thr Ser Arg Asn Ala Ala Ile His Tyr Gly Ile Tyr Pro Lys Lys Gly

370 375 380

Ser Met Glu Ile Gly Ser Asp Ala Asp Phe Thr Val Ile Asp Leu Glu

385 390 395 400

Lys Glu Tyr Val Ile Asp Glu Gln Lys Thr Glu Ser Met Ala Lys Tyr

405 410 415

Asn Pro Leu His Gly Met Lys Leu Lys Gly Lys Pro Val Gln Thr Ile

420 425 430

Ile Arg Gly Asn Val Ile Phe Asp Glu Asp Lys Gly Ile Val Gly Glu

435 440 445

Ala Gly Tyr Gly Glu Tyr Val Pro Arg Gln Ser Ile Gln Arg Leu Glu

450 455 460

Arg Thr Phe Lys Tyr Glu Lys Tyr Glu Ala Val Lys Ala Lys Thr Lys

465 470 475 480

Asp Pro Val Ala Gln Gln

485

<210> 9

<211> 470

<212> PRT

<213> 类芽孢杆菌(Paenibacillus sp. )

<400> 9

Met Gly Tyr Glu Leu Ile Ile Lys Asn Ala Ser Ile Pro Gln Gly Asp

1 5 10 15

Arg Gln Val Leu Thr Asp Ile Leu Val Asn Lys Gly Val Ile Val Gly

20 25 30

Phe Ala Lys Asp Ile Asp Ala Glu Ala Asp Thr Ile Ile Asp Ala Gln

35 40 45

Gly Lys Leu Val Leu Pro Gly Val Ile Asp Ser His Thr His Phe Asn

50 55 60

Asp Pro Gly Phe Thr His Arg Glu Asp Phe Val Thr Gly Thr Ser Ser

65 70 75 80

Ala Ala Ala Gly Gly Val Thr Met Ile Val Asp Met Pro Cys Cys Ser

85 90 95

Val Pro Ser Val Arg Ser Val Glu Asn Leu His Asn Lys Leu Asn Ala

100 105 110

Leu Glu Gly Lys Gly Ile Val Asp Tyr Ala Met Trp Gly Gly Ile Thr

115 120 125

Gly Glu Asp Val Arg Asn Asp Val Leu Asp Ile Val Lys Glu Gln Ala

130 135 140

Ala Glu Gly Val Val Ala Phe Lys Val Tyr Met Thr Pro Ser Val Pro

145 150 155 160

Ser Tyr Pro Arg Val Thr Asp Pro Glu Met Leu Glu Ala Phe Tyr Ala

165 170 175

Val Ala Glu Thr Gly Met Pro Ile Gly Ile His Ala Glu Asn Tyr Ala

180 185 190

Ile Cys Asp Tyr Tyr Thr Gln Lys Leu Lys Lys Glu Gly Arg Met Asp

195 200 205

Tyr Pro Ala Trp Ala Glu Ala Arg Lys Ile Leu Ala Glu Lys Ala Ala

210 215 220

Ile Gln Leu Gly Ile Ser Phe Ser Glu Glu Ser Gly Ala Arg Leu His

225 230 235 240

Ile Val His Met Ser Thr Ala Ala Gly Asn Gln Leu Ile Lys Glu Ala

245 250 255

Lys Leu Lys Gly Val Lys Val Thr Ala Glu Ser Cys Pro His Tyr Leu

260 265 270

Thr Leu Asn Ala Val Asp Ala Met Thr Glu Phe Gly Thr Phe Ala Lys

275 280 285

Ile Ala Pro Pro Leu Arg Arg Thr Glu Asp Asn Glu Ala Leu Trp Gln

290 295 300

Gly Leu Val Asp Gly Thr Ile Asp Phe Met Ala Thr Asp His Ala Pro

305 310 315 320

Tyr Glu Ile Ala Thr Glu Lys Asp Ala Pro Gly Met Asp Val Trp Thr

325 330 335

Ser Phe Pro Gly Ile Pro Gly Val Glu Thr Leu Val Pro Ile Met Ile

340 345 350

Ser Glu Gly Tyr Asn Lys Gly Arg Leu Ser Leu Ser Arg Leu Val Glu

355 360 365

Leu Leu Cys Thr Asn Pro Ala Ile His Tyr Gly Leu Tyr Pro Lys Lys

370 375 380

Gly Ala Leu Glu Ile Gly Thr Asp Ala Asp Phe Thr Ile Val Asp Leu

385 390 395 400

Glu Lys Glu Trp Thr Leu Asp Lys Asp Lys Met His Cys Lys Pro Lys

405 410 415

Tyr Thr Pro Phe His Gly Met Lys Leu Lys Gly Lys Val Asp Lys Thr

420 425 430

Ile Val Arg Gly Thr Leu Val Tyr Asp Asp Glu Leu Gly Val Val Gly

435 440 445

Lys Pro Gly Tyr Gly Glu Phe Val Lys Arg Gln Thr Ile Ser Glu Leu

450 455 460

Pro Arg Leu Leu Lys Tyr

465 470

<210> 10

<211> 472

<212> PRT

<213> 双岐高温黄色微球菌(Thermoflavimicrobium dichotomicum)

<400> 10

Met Gln Leu Ile Leu Lys Asn Ala Thr Ile Pro Gln Gly Phe Gly Asn

1 5 10 15

Arg Thr Val Lys Thr Asn Ile Leu Val Asp Asp Asn Lys Ile Val Gly

20 25 30

Phe Thr Asn Cys Ile Asp Gly Ile Glu Ala Lys Glu Val Ile Asp Met

35 40 45

Asp Gly Lys Leu Val Val Pro Gly Cys Ile Asp Pro His Thr His Phe

50 55 60

Met Asp Pro Gly Phe Thr His Arg Glu Thr Phe Ala Thr Gly Ser Ala

65 70 75 80

Ser Ala Ala Ala Gly Gly Leu Thr Cys Ile Val Asp Met Pro Cys Cys

85 90 95

Ser Lys Pro Ser Val Arg Ser Arg Asp Glu Phe Tyr Gln Lys Leu Asn

100 105 110

Pro Ile Lys Asp Gln Ser Tyr Ile Asp Phe Ala Phe Trp Gly Gly Met

115 120 125

Thr Gly Glu Asp Val Arg Glu Gly Lys Leu Asp Asn Val Trp Gly Gln

130 135 140

Val Glu Glu Gly Val Thr Ala Phe Lys Val Tyr Met Thr Pro Ser Val

145 150 155 160

Pro Thr Phe Pro Lys Val Ser Asp Ala Glu Met Leu Glu Ile Phe Ala

165 170 175

Lys Val Ala Glu Thr Gly Leu Pro Val Gly Val His Ala Glu Asn Tyr

180 185 190

Asp Ile Cys Ser Phe Arg Val Glu Lys Leu Lys Lys Gln Gly Arg Met

195 200 205

Asp Gly Pro Ala Trp Ala Glu Ala Arg Asn Ser Leu Ala Glu Lys Val

210 215 220

Ala Ile Glu Leu Val Ile Ser Phe Ala Glu Ala Thr Gly Ala Arg Ala

225 230 235 240

His Ile Val His Met Ser Thr Lys Glu Gly Val Gly Leu Val Arg Gln

245 250 255

Ala Lys Leu Arg Gly Leu Pro Ile Thr Ala Glu Thr Cys Pro His Tyr

260 265 270

Leu Val Leu Asn Ala Gln Glu Ala Met Thr Glu Phe Gly Thr Phe Ala

275 280 285

Lys Ile Ala Pro Pro Leu Arg Gly Lys Glu Asp Asn Glu Val His Trp

290 295 300

Lys Ala Leu Ala Asp Gly Thr Ile Asp Phe Val Ala Thr Asp His Ala

305 310 315 320

Pro Tyr Glu Ile Pro Thr Glu Lys Glu Gly Glu Gly Leu Asp Ile Trp

325 330 335

Thr Ala Phe Pro Gly Ile Pro Gly Val Glu Gln Met Val Pro Ile Leu

340 345 350

Val Ser Glu Gly Leu Asn Lys Gly Arg Leu Thr Leu Gln Arg Phe Val

355 360 365

Glu Val Thr Ser Thr Asn Ala Ala Ile His Phe Gly Leu Tyr Pro Lys

370 375 380

Lys Gly Ser Met Glu Ile Gly Thr Asp Ala Asp Phe Thr Val Ile Asp

385 390 395 400

Leu Glu Arg Glu Tyr Val Ile Asp Lys Asp Lys Val His Ser Met Ala

405 410 415

Lys Tyr Thr Pro Phe His Gly Met Lys Leu Lys Gly Lys Pro Val Met

420 425 430

Thr Ile Val Arg Gly Thr Val Val Tyr Asp Glu Asp His Gly Gly Leu

435 440 445

Val Gly Lys Lys Gly Tyr Gly Gln Phe Val Lys Arg Gln Ser Asn Ile

450 455 460

Ala Leu Pro Arg Gln Leu Gln Phe

465 470

<210> 11

<211> 490

<212> PRT

<213> Fictibacillus enclensis

<400> 11

Met Asp Leu Ile Leu Lys Asn Ala Met Ile Pro Gln Gly Asp Arg Thr

1 5 10 15

Val Ile Thr Asn Ile Leu Val His Glu Gly Arg Ile Thr Gly Phe Cys

20 25 30

Asn Asp Ile Gln Thr Leu Asn Ala Asp Lys Val Leu Asp Met Glu Gly

35 40 45

Lys Leu Val Val Pro Gly Cys Ile Asp Pro His Thr His Phe Met Asp

50 55 60

Pro Gly Phe Thr His Arg Glu Thr Phe Ala Thr Gly Ser Arg Ser Ala

65 70 75 80

Ala Ala Gly Gly Leu Thr Thr Ile Ile Asp Met Pro Cys Cys Ser Lys

85 90 95

Pro Ser Val Arg Asp Gly Glu Ser Phe Gln Lys Lys Ile Ala Pro Ile

100 105 110

Arg Gly Gln Ala Tyr Ile Asp Tyr Cys Phe Trp Gly Gly Met Thr Gly

115 120 125

Glu Asp Ala Arg Asn Gly Trp Ile Asp Asn Ile Tyr Pro Gln Ile Asp

130 135 140

Glu Gly Val Val Ala Phe Lys Val Tyr Met Thr Pro Ser Val Pro Thr

145 150 155 160

Phe Pro Lys Val Ser Asp Gly Glu Met Leu Glu Ile Phe Gln Lys Val

165 170 175

Ala Lys Thr Gly Met Pro Ile Gly Val His Ala Glu Asn Tyr Asp Ile

180 185 190

Cys Thr Phe Tyr Thr Glu Lys Leu Lys Asn Glu Gly Arg Glu Asp Gly

195 200 205

Pro Ala Trp Ala Glu Ala Arg Met Ala Leu Ala Glu Lys Val Ala Ile

210 215 220

Glu Leu Ile Ile Ser Tyr Ala Glu Ala Thr Asp Ala Arg Val His Val

225 230 235 240

Val His Met Ser Thr Lys Glu Gly Val Lys Leu Val Arg Glu Ala Lys

245 250 255

Lys Arg Gly Val Lys Val Thr Ser Glu Thr Cys Pro His Tyr Leu Met

260 265 270

Leu Asn Ala Gln Asp Ser Met Thr Glu Arg Gly Thr Phe Ala Lys Ile

275 280 285

Ala Pro Pro Leu Arg Gly Lys Asp Asp Asn Gln Ala His Trp Gln Ala

290 295 300

Leu Ala Asp Gly Thr Ile Asp Phe Val Gly Thr Asp His Ala Pro Tyr

305 310 315 320

Glu Ile Ala Thr Glu Lys Ala Ala Ala Asp Ser Ser Ile Trp Asn Ser

325 330 335

Phe Pro Gly Ile Pro Gly Val Glu Thr Met Val Pro Ile Leu Val Ser

340 345 350

Glu Gly Leu Asn Lys Gly Arg Leu Ser Leu Ser Arg Phe Val Glu Val

355 360 365

Thr Ser Lys Asn Ala Ala Val His Tyr Gly Ile Tyr Pro Lys Lys Gly

370 375 380

Ala Met Glu Ile Gly Ser Asp Ala Asp Phe Thr Val Ile Asp Leu Asp

385 390 395 400

His Glu Trp Val Ile Asp Glu Lys Gln Thr Glu Ser Met Ala Lys Tyr

405 410 415

Asn Pro Leu His Gly Ile Lys Leu Lys Gly Arg Pro Val Arg Thr Ile

420 425 430

Leu Arg Gly Thr Val Val Tyr Asp Tyr Glu Lys Gly Gly Ile Thr Gly

435 440 445

Thr Ala Gly Tyr Gly Glu Tyr Val Lys Arg Gln Ser Ile Gln Arg Leu

450 455 460

Glu Arg Met Leu Lys Tyr Glu Asn Tyr Glu Thr Ala Asn Gln Pro Ser

465 470 475 480

Ala Ala Arg Gln Gln Leu Glu Lys Ser Lys

485 490

<210> 12

<211> 473

<212> PRT

<213> 中温海洋丝状菌(Marininema mesophilum)

<400> 12

Met Asp Leu Leu Leu Lys Asn Ala Asn Ile Pro Gln Gly Asp Arg Gln

1 5 10 15

Val Gln Thr Asn Ile Leu Val Asp Gly Gly Ser Ile Val Gly Tyr Val

20 25 30

Gln Asp Ala Ser Gly Val Ile Ala Glu Arg Thr Ile Asp Leu Asp Gly

35 40 45

Lys Leu Val Val Pro Gly Cys Ile Asp Pro His Thr His Phe Met Asp

50 55 60

Pro Gly Phe Thr His Arg Glu Thr Phe Ala Glu Ala Ser Met Ser Ala

65 70 75 80

Ala Ala Gly Gly Leu Thr Ser His Val Asp Met Pro Cys Cys Ser Lys

85 90 95

Pro Ser Val Arg Ser Glu Asp Glu Leu Tyr Met Lys Leu Asn Pro Ile

100 105 110

Lys Asp Gln Ala Tyr Ile Asp Phe Ala Phe Trp Gly Gly Met Thr Gly

115 120 125

Glu Asp Val Arg Glu Gly Lys Leu Asp Asn Val Trp Gly Gln Val Asp

130 135 140

Ala Gly Val Thr Ala Phe Lys Val Tyr Met Thr Pro Ser Val Pro Thr

145 150 155 160

Phe Pro Arg Val Gly Asp Ala Glu Met Leu Glu Ile Phe Arg Lys Val

165 170 175

Ala Glu Thr Gly Leu Pro Val Gly Val His Ala Glu Asn Tyr Asp Ile

180 185 190

Cys Ser Phe Tyr Ala Asn Lys Leu Glu Lys Glu Gly Arg Thr Asp Ser

195 200 205

Pro Ala Trp Ala Glu Ala Arg Met Ala Leu Ala Glu Lys Ala Ala Ile

210 215 220

Gln Leu Ile Ile Ser Phe Ala Glu Glu Thr Gly Ala Arg Ala His Ile

225 230 235 240

Val His Met Ser Thr Lys Glu Gly Ala Val Leu Ile Arg Glu Ala Lys

245 250 255

Arg Arg Gly Val Lys Ile Thr Ala Glu Ala Cys Pro His Tyr Leu Thr

260 265 270

Leu Asn Ala Glu Glu His Met Thr Lys Phe Gly Ala Leu Ala Lys Ile

275 280 285

Ala Pro Pro Leu Arg Gly Lys Glu Asp Asn Glu Val His Trp Gln Ala

290 295 300

Leu Ala Asp Gly Thr Ile Asp Phe Val Gly Thr Asp His Ala Pro Tyr

305 310 315 320

Glu Ile Ala Thr Glu Lys Ile Ala Glu Gly Leu His Ile Trp Asn Ser

325 330 335

Phe Pro Gly Ile Pro Gly Val Glu Thr Met Val Pro Ile Met Val Ser

340 345 350

Glu Gly Leu Asn Lys Gly Arg Leu Ser Leu Ser Arg Phe Val Glu Val

355 360 365

Thr Ser Arg Asn Ala Ala Ile His Phe Gly Leu Tyr Pro Lys Lys Gly

370 375 380

Thr Met Glu Leu Gly Ser Asp Ala Asp Phe Thr Val Ile Asp Leu Glu

385 390 395 400

Arg Glu Tyr Glu Ile Asp Gln Glu Lys Thr Leu Thr Lys Ala Lys Tyr

405 410 415

Thr Pro Phe His Gly Met Lys Leu Lys Gly Lys Pro Val Met Thr Ile

420 425 430

Val Arg Gly Gly Val Val Tyr Asp Glu Asp Arg Gly Gly Ile Val Gly

435 440 445

Thr Ala Gly Tyr Gly Gln Phe Val Lys Arg Gln Ser Asn Ile Glu Leu

450 455 460

Lys Arg Val Glu Gly Ser Leu Val Tyr

465 470

<210> 13

<211> 470

<212> PRT

<213> 类芽孢杆菌(Paenibacillus sp.)

<400> 13

Met Gly Tyr Glu Leu Ile Ile Lys Asn Ala Ser Ile Pro Gln Gly Asp

1 5 10 15

Arg Gln Val Leu Thr Asp Ile Leu Val Asn Asn Gly Val Ile Val Gly

20 25 30

Phe Ala Lys Asp Ile Asp Ala Glu Ala Asp Thr Ile Ile Asp Ala Gln

35 40 45

Gly Lys Leu Val Leu Pro Gly Val Ile Asp Ser His Thr His Phe Asn

50 55 60

Asp Pro Gly Phe Thr His Arg Glu Asp Phe Val Thr Gly Thr Ser Ser

65 70 75 80

Ala Ala Ala Gly Gly Val Thr Met Ile Val Asp Met Pro Cys Cys Ser

85 90 95

Val Pro Ser Val Arg Ser Val Glu Asn Leu His Asn Lys Leu Asn Ala

100 105 110

Leu Glu Gly Lys Gly Ile Val Asp Tyr Ala Met Trp Gly Gly Ile Thr

115 120 125

Gly Glu Asp Val Arg Asn Asp Val Leu Asp Ile Val Lys Glu Gln Ala

130 135 140

Ala Glu Gly Val Val Ala Phe Lys Val Tyr Met Thr Pro Ser Val Pro

145 150 155 160

Ser Tyr Pro Arg Val Thr Asp Pro Glu Met Leu Glu Ala Phe Tyr Ala

165 170 175

Val Ala Glu Thr Gly Met Pro Ile Gly Ile His Ala Glu Asn Tyr Ala

180 185 190

Ile Cys Asp Tyr Tyr Thr Gln Lys Leu Lys Lys Glu Gly Arg Met Asp

195 200 205

Tyr Pro Ala Trp Ala Glu Ala Arg Lys Ile Leu Ala Glu Lys Ala Ala

210 215 220

Ile Gln Leu Gly Ile Ser Phe Ser Glu Glu Thr Gly Ala Arg Leu His

225 230 235 240

Ile Val His Met Ser Thr Ala Ala Gly Asn Gln Leu Ile Lys Glu Ala

245 250 255

Lys Leu Lys Gly Val Lys Val Thr Ala Glu Ser Cys Pro His Tyr Leu

260 265 270

Thr Leu Asn Ala Val Asp Ala Met Thr Glu Phe Gly Thr Phe Ala Lys

275 280 285

Ile Ala Pro Pro Leu Arg Arg Pro Glu Asp Asn Glu Ala Leu Trp Gln

290 295 300

Gly Leu Val Asp Gly Thr Ile Asp Phe Met Ala Thr Asp His Ala Pro

305 310 315 320

Tyr Glu Ile Ala Thr Glu Lys Asp Ala Pro Gly Met Asp Val Trp Thr

325 330 335

Ser Phe Pro Gly Ile Pro Gly Val Glu Thr Leu Val Pro Ile Met Ile

340 345 350

Ser Glu Gly Tyr Asn Lys Gly Arg Leu Ser Leu Ser Arg Leu Val Glu

355 360 365

Leu Leu Cys Thr Asn Pro Ala Ile His Tyr Gly Leu Tyr Pro Lys Lys

370 375 380

Gly Ala Leu Glu Ile Gly Thr Asp Ala Asp Phe Thr Ile Val Asp Leu

385 390 395 400

Glu Lys Glu Trp Thr Leu Asp Lys Asp Lys Met His Cys Lys Pro Lys

405 410 415

Tyr Thr Pro Phe His Gly Met Lys Leu Lys Gly Lys Val Asp Lys Thr

420 425 430

Ile Val Arg Gly Thr Leu Val Tyr Asp Asp Glu Leu Gly Val Val Gly

435 440 445

Lys Pro Gly Tyr Gly Glu Phe Val Lys Arg Gln Thr Ile Ser Glu Leu

450 455 460

Pro Arg Leu Leu Lys Tyr

465 470

<210> 14

<211> 471

<212> PRT

<213> 香蒲类芽孢杆菌(Paenibacillus typhae)

<400> 14

Met Ser Tyr Asp Leu Ile Ile Lys Asn Ala Asn Ile Pro Gln Gly Asp

1 5 10 15

Arg Gln Val Leu Thr Asp Ile Leu Val Asn Asn Gly Val Ile Val Gly

20 25 30

Phe Ala Lys Asp Ile Asp Ala Glu Ala Ala Glu Thr Ile Asp Ala Gly

35 40 45

Gly Lys Leu Val Leu Pro Gly Val Ile Asp Ser His Thr His Phe Asn

50 55 60

Asp Pro Gly Phe Thr His Arg Glu Asp Phe Val Thr Gly Thr Ser Ser

65 70 75 80

Ala Ala Ala Gly Gly Val Thr Met Ile Val Asp Met Pro Cys Cys Ser

85 90 95

Val Pro Ser Val Arg Ser Ala Asp Asn Leu Phe Asn Lys Leu Asn Ala

100 105 110

Leu Glu Gly Lys Gly Ile Val Asp Tyr Ala Met Trp Gly Gly Ile Thr

115 120 125

Gly Glu Asp Val Arg Asn Asn Val Leu Pro Glu Ile Ile Ser Glu Gln

130 135 140

Val Ala Asn Gly Val Val Ala Phe Lys Val Tyr Met Thr Pro Ser Val

145 150 155 160

Pro Ser Tyr Pro Arg Val Thr Asp Pro Glu Met Leu Glu Ala Phe Ala

165 170 175

Ala Val Ser Pro Thr Gly Leu Pro Ile Gly Ile His Ala Glu Asn Phe

180 185 190

Ala Ile Cys Asp Tyr Phe Thr Asn Lys Leu Lys Gly Glu Gly Arg Leu

195 200 205

Asp Tyr Pro Ala Trp Ala Glu Ala Arg Met Ala Leu Ala Glu Lys Ala

210 215 220

Ala Ile Gln Leu Gly Ile Ser Phe Ala Glu Glu Thr Gly Ala Arg Leu

225 230 235 240

His Ile Val His Met Ser Thr Gly Ala Gly Asn Glu Leu Ile Arg Glu

245 250 255

Ala Lys Leu Lys Gly Thr Lys Val Thr Ala Glu Ser Cys Pro His Tyr

260 265 270

Leu Thr Leu Asn Ala Val Asp Ala Met Thr Glu Phe Gly Thr Phe Ala

275 280 285

Lys Ile Ala Pro Pro Leu Arg Thr Ala Lys Asp Asn Glu Val Leu Trp

290 295 300

Gln Gly Leu Ala Asp Gly Thr Ile Asp Phe Met Ala Thr Asp His Ala

305 310 315 320

Pro Tyr Glu Ile Ala Thr Glu Lys Asp Ala Pro Gly Met Asp Val Trp

325 330 335

Thr Ser Phe Pro Gly Ile Pro Gly Val Glu Thr Leu Val Pro Ile Met

340 345 350

Ile Ser Glu Gly Tyr Asn Lys Gly Arg Leu Ser Leu Ser Arg Leu Val

355 360 365

Glu Leu Leu Cys Thr Asn Pro Ala Val His Tyr Gly Leu Tyr Pro Lys

370 375 380

Lys Gly Ala Leu Glu Ile Gly Thr Asp Ala Asp Phe Thr Ile Val Asp

385 390 395 400

Leu Glu Lys Glu Trp Thr Leu Asp Lys Asp Gln Met His Cys Lys Pro

405 410 415

Lys Tyr Thr Pro Phe His Gly Met Lys Leu Lys Gly Lys Val Asp Lys

420 425 430

Thr Ile Val Arg Gly Thr Leu Val Tyr Asp Glu Ala Leu Gly Val Val

435 440 445

Gly Lys Pro Gly His Gly Glu Tyr Val Lys Arg Gln Thr Ile Ser Glu

450 455 460

Leu Pro Arg Leu Leu Lys Tyr

465 470

<210> 15

<211> 532

<212> PRT

<213> 极尖组织菌(Tissierella praeacuta)

<400> 15

Met Tyr Asp Val Ile Val Lys Asn Ala Arg Ile Pro Gln Gly Asp Asp

1 5 10 15

Thr Ala Leu Thr Asn Ile Leu Val Lys Asp Glu Lys Ile Ala Gly Phe

20 25 30

Val Asp Asn Ile Glu Gly Ile Glu Ala Lys Glu Ile Ile Asp Ala Lys

35 40 45

Gly His Leu Thr Leu Pro Gly Cys Ile Asp Ser His Thr His Phe Met

50 55 60

Tyr Gln Gly Phe Pro His Arg Glu Asn Phe Leu Thr Gly Thr Ala Ala

65 70 75 80

Ala Ala Arg Gly Gly Val Thr Thr Val Ile Asp Met Pro Cys Cys Ser

85 90 95

Val Pro Ser Ala Arg Ser Lys Glu Gln Leu Glu Leu Lys Leu Asp Leu

100 105 110

Ile Gln Pro Gln Ala Leu Val Asp Phe Ala Leu Trp Gly Gly Val Thr

115 120 125

Gly Glu Asp Val Arg Glu Gly Trp Leu Asp His Val Gln Glu Gln Ala

130 135 140

Asp Trp Gly Val Val Ala Phe Lys Ala Tyr Met Thr Pro Ser Val Pro

145 150 155 160

Thr Phe Pro Arg Val Thr Asp Pro Glu Met Phe Glu Ala Phe Lys Ala

165 170 175

Val Ala Lys Thr Gly Leu Pro Val Gly Ile His Ala Glu Asn Tyr Ser

180 185 190

Met Cys Asp Tyr Tyr Val Lys Glu Phe Gln Lys Gln Gly Arg Met Asp

195 200 205

Gly Pro Ala Trp Ala Glu Ala Arg Met Glu Leu Ala Glu Lys Val Ala

210 215 220

Ile Glu Leu Gly Ile Ser Phe Ala Glu Glu Ala Gly Ala Arg Leu His

225 230 235 240

Ile Val His Met Ser Thr Gly Ile Gly Ala Lys Leu Val Gly Glu Ala

245 250 255

Lys Lys Arg Gly Leu Asn Val Thr Ser Glu Thr Cys Pro His Tyr Leu

260 265 270

Thr Ile Asn Ala Gln Glu Ala Met Thr Lys Tyr Gly Ala Cys Ala Lys

275 280 285

Ile Ala Pro Pro Leu Arg Thr Lys Lys Asp Asn Glu Leu Leu Trp Glu

290 295 300

Gly Leu Asn Asn Gly Ser Val Asp Phe Ile Ala Thr Asp His Ala Pro

305 310 315 320

Tyr Glu Leu Glu Ser Glu Lys Ile Lys Glu Gly Thr Asn Ile Trp Thr

325 330 335

Ala Phe Pro Gly Ile Pro Gly Val Glu Thr Met Val Pro Val Leu Val

340 345 350

Ser Glu Gly Tyr Asn Lys Gly Arg Ile Ser Leu Ser Thr Leu Val Asp

355 360 365

Val Leu Ser Lys Asn Ala Ala Val His Tyr Gly Leu Tyr Pro Lys Lys

370 375 380

Gly Ala Met Asn Ile Gly Ser Asp Ala Asp Phe Thr Ile Ile Asp Leu

385 390 395 400

Asp Lys Glu Trp Val Ile Asp Pro Lys Asn Glu Ala Ser Met Cys Gly

405 410 415

Tyr Thr Pro Leu Glu Gly Ile Lys Leu Lys Gly Lys Val Ala Lys Thr

420 425 430

Ile Val Arg Gly His Leu Val Phe Glu Asp Leu Asp Glu Ser Thr Leu

435 440 445

Ala Lys Leu Thr Asp Glu Glu Leu Lys Lys Ile Val His Asn Phe Pro

450 455 460

Glu Gly Ile Glu Glu Lys Tyr Ala Glu Gln Phe Lys Lys Tyr Pro His

465 470 475 480

Leu Tyr Ser Ala Glu Tyr Glu Arg Ser Tyr Arg Ile Asn His Pro Glu

485 490 495

Val Ile Asp Glu His Ile Arg Gly Ile Lys Gly Ile Val Ala Lys Pro

500 505 510

Gly Phe Gly Lys Phe Val Lys Arg Gln Ser Ile Gln Ile Leu Pro Arg

515 520 525

Lys Ile Lys Tyr

530

<210> 16

<211> 495

<212> PRT

<213> 芽孢杆菌(Bacillus sp. )

<400> 16

Met Asp Leu Ile Leu Lys Asn Ala Asn Ile Pro Gln Gly Asp Arg Gln

1 5 10 15

Val Leu Thr Asn Ile Leu Val Asn Asp Gly Lys Ile Thr Gly Phe Val

20 25 30

Ser Asn Ile Thr Gly Leu Val Ala Asp Lys Val Ile Asp Cys Lys Gly

35 40 45

Tyr Leu Val Val Pro Gly Cys Ile Asp Pro His Thr His Phe Met Asp

50 55 60

Pro Gly Phe Thr His Arg Glu Thr Phe Ala Thr Gly Ser Arg Ser Ala

65 70 75 80

Ala Ala Gly Gly Val Thr Thr Ile Ile Asp Met Pro Cys Cys Ser Lys

85 90 95

Pro Ser Val Arg Asp Lys Glu Ser Phe His Lys Lys Ile Gly Pro Ile

100 105 110

Arg Asp Gln Ala Tyr Ile Asp Tyr Cys Phe Trp Gly Gly Val Thr Gly

115 120 125

Glu Asp Val Arg Glu Gly Trp Leu Asp Asn Ile Tyr Pro Gln Ile Glu

130 135 140

Glu Gly Val Val Ala Phe Lys Val Tyr Met Thr Pro Ser Val Pro Thr

145 150 155 160

Tyr Pro Lys Val Ser Asp Gly Glu Met Leu Glu Ile Phe Arg Lys Val

165 170 175

Ala Pro Thr Gly Leu Ala Ile Gly Val His Ala Glu Asn Tyr Asp Ile

180 185 190

Cys Thr Phe Asp Thr Glu Lys Leu Lys Lys Ala Gly Arg Leu Asp Gly

195 200 205

Pro Ala Trp Ala Glu Ala Arg Ser Ser Leu Ala Glu Lys Val Ala Ile

210 215 220

Gln Leu Ile Ile Ser Tyr Ala Glu Ala Thr Asp Ala Arg Val His Val

225 230 235 240

Val His Met Ser Thr Lys Glu Gly Val Glu Leu Val Arg Glu Ala Lys

245 250 255

Lys Arg Gly Val Lys Val Thr Ser Glu Thr Cys Pro His Tyr Leu Met

260 265 270

Leu Asn Ala Gln Asp Ala Met Ser Glu Arg Gly Thr Phe Ala Lys Ile

275 280 285

Ala Pro Pro Leu Arg Gly Lys Glu Asp Asn Glu Glu His Trp Gln Ala

290 295 300

Leu Ala Asp Gly Thr Ile Asp Phe Val Gly Thr Asp His Ala Pro Tyr

305 310 315 320

Glu Ile Ala Thr Glu Lys Ala Ala Ala Asp Ser Asp Ile Trp Asn Ser

325 330 335

Phe Pro Gly Ile Pro Gly Val Glu Thr Met Val Gln Ile Leu Val Ser

340 345 350

Glu Gly Leu Asn Lys Gly Arg Leu Ser Leu Ser Arg Phe Val Glu Val

355 360 365

Thr Ser Arg Asn Ala Ala Val His Tyr Gly Ile Tyr Pro Lys Lys Gly

370 375 380

Ala Met Glu Ile Gly Ser Asp Ala Asp Phe Thr Ile Ile Asp Leu Asn

385 390 395 400

Arg Glu Tyr Thr Ile Asp Glu Gln Lys Thr Glu Ser Met Cys Lys Tyr

405 410 415

Asn Pro Leu His Gly Leu Gln Leu Lys Gly Lys Pro Val Gln Thr Val

420 425 430

Ile Arg Gly Glu Leu Ile Tyr Asp Glu Asp Asn Gly Gly Ile Val Gly

435 440 445

Lys Ala Gly Tyr Gly Glu Tyr Val Lys Arg Gln Ser Ile Gln Arg Leu

450 455 460

Asp Arg Val Leu Lys Tyr Glu Thr Tyr Glu Ala Lys Gly Asn Asp Ala

465 470 475 480

Ala Asp Gln Lys Glu Ala Val Leu Ser Gln Ser Ser Asp Arg Lys

485 490 495

<210> 17

<211> 469

<212> PRT

<213> 慢生型大豆根瘤菌(Bradyrhizobium japonicum)

<400> 17

Met Asp Leu Ile Leu Lys Asn Ala Asn Ile Pro Gln Gly Asp Arg Gln

1 5 10 15

Val Leu Thr Asn Ile Leu Val Glu Asn Glu Arg Ile Val Gly Phe Val

20 25 30

Asn Ser Ile Asp His Ile Glu Ala Lys Gln Ala Ile Asp Val Asn Gly

35 40 45

Lys Leu Val Val Pro Gly Cys Ile Asp Pro His Thr His Phe Met Asp

50 55 60

Pro Gly Phe Thr His Arg Glu Thr Phe Ala Thr Gly Thr Met Ser Ala

65 70 75 80

Ala Ala Gly Gly Val Thr Thr Ile Ile Asp Met Pro Cys Cys Ser Lys

85 90 95

Pro Ser Val Arg Ser Asp Glu Thr Leu Phe Ala Lys Leu Asn Pro Leu

100 105 110

Leu Glu Gln Ala His Ile Asp Phe Cys Phe Trp Gly Gly Met Thr Gly

115 120 125

Glu Asp Val Arg Glu Gly Trp Leu Asp Asn Val Trp Gly Gln Val Glu

130 135 140

Gln Gly Val Val Ala Phe Lys Val Tyr Met Thr Pro Ser Val Pro Thr

145 150 155 160

Phe Pro Arg Val Ser Asp Ala Glu Met Leu Glu Ile Phe Tyr Lys Val

165 170 175

Ala Glu Thr Gly Leu Pro Val Gly Val His Ala Glu Asn Tyr Asp Ile

180 185 190

Cys Ser Phe Tyr Thr Glu Lys Leu Lys Lys Gln Gly Arg Met Asp Gly

195 200 205

Pro Ala Trp Ala Glu Ala Arg Lys Ala Leu Ala Glu Lys Val Ala Ile

210 215 220

Glu Leu Ile Ile Ser Tyr Ala Glu Glu Thr Gly Ala Arg Val His Val

225 230 235 240

Val His Met Ser Thr Lys Glu Gly Ala His Val Val Arg Glu Ala Lys

245 250 255

Lys Arg Gly Val Lys Val Thr Ala Glu Thr Cys Pro His Tyr Leu Met

260 265 270

Leu Asn Ala Glu Glu Ala Met Thr Glu His Gly Thr Phe Ala Lys Ile

275 280 285

Ala Pro Pro Leu Arg Gly Lys Glu Asp Asn Glu Val His Trp Lys Ala

290 295 300

Leu Ala Asp Gly Thr Ile Asp Phe Val Gly Thr Asp His Ala Pro Tyr

305 310 315 320

Glu Ile Ala Thr Glu Lys Ala Ala Glu Asp Ser Asp Ile Trp Asn Ser

325 330 335

Phe Pro Gly Ile Pro Gly Val Glu Thr Met Val Pro Ile Leu Val Ser

340 345 350

Glu Gly Leu Asn Lys Gly Arg Leu Ser Leu Ser Arg Phe Val Glu Val

355 360 365

Thr Ser Arg Asn Ala Ala Ile His Tyr Gly Ile Tyr Pro Lys Lys Gly

370 375 380

Ala Met Glu Ile Gly Ser Asp Ala Asp Phe Thr Val Ile Asp Leu Glu

385 390 395 400

Lys Glu His Thr Ile Asp Gln Ala Gln Thr Tyr Ser Met Ala Lys Tyr

405 410 415

Asn Pro Leu His Gly Ile Gln Leu Lys Gly Met Pro Val Met Thr Ile

420 425 430

Val Arg Gly Thr Val Val Tyr Glu Asp Gly Lys Gly Ile Val Gly Lys

435 440 445

Pro Gly Tyr Gly Lys Phe Thr Lys Arg Glu Ser Ile Gln Gln Leu Asp

450 455 460

Arg Thr Leu Lys Tyr

465

<210> 18

<211> 486

<212> PRT

<213> 坚强芽孢杆菌(Bacillus firmus)

<400> 18

Met Asp Leu Ile Leu Lys Asn Ala Asn Ile Pro Gln Gly Asp Arg Lys

1 5 10 15

Val Leu Thr Asn Ile Leu Val Asn Glu Gly Lys Ile Val Gly Tyr Thr

20 25 30

Asn Asp Ile Ser Phe Leu Asn Ala Ala Lys Val Ile Asp Ile Gln Gly

35 40 45

Lys Leu Val Val Pro Gly Cys Ile Asp Pro His Thr His Phe Met Asp

50 55 60

Pro Gly Phe Thr His Arg Glu Thr Phe Ala Thr Gly Ser Arg Ser Ala

65 70 75 80

Ala Ala Gly Gly Leu Thr Thr Ile Ile Asp Met Pro Cys Cys Ser Lys

85 90 95

Pro Ser Val Arg Asp Gly Asp Ser Phe Asn Lys Lys Ile Gly Pro Ile

100 105 110

Arg Asp Gln Ala Tyr Ile Asp Tyr Cys Phe Trp Gly Gly Met Thr Gly

115 120 125

Glu Asp Ala Arg Glu Gly Trp Ile Asp Asn Ile Tyr Pro Gln Ile Asp

130 135 140

Gln Gly Val Val Ala Phe Lys Val Tyr Met Thr Pro Ser Val Pro Thr

145 150 155 160

Phe Pro Lys Val Ser Asp Ala Glu Met Leu Glu Ile Phe Lys Asn Val

165 170 175

Ala Lys Thr Gly Leu Pro Ile Gly Val His Ala Glu Asn Tyr Asp Ile

180 185 190

Cys Thr Phe Asn Ser Lys Lys Leu Glu Lys Glu Gly Arg Leu Asp Gly

195 200 205

Pro Ala Trp Ala Glu Ala Arg Ser Ser Leu Ala Glu Lys Val Ala Ile

210 215 220

Glu Leu Ile Leu Ser Phe Ala Glu Ala Thr Asp Ala Arg Val His Val

225 230 235 240

Val His Met Ser Thr Lys Glu Gly Val Glu Leu Val Lys Ala Ala Lys

245 250 255

Lys Arg Gly Val Lys Val Thr Ala Glu Thr Cys Pro His Tyr Leu Val

260 265 270

Leu Asn Ala Gln Asp Ser Met Ser Glu Arg Gly Ser Tyr Ala Lys Ile

275 280 285

Ala Pro Pro Leu Arg Gly Lys Glu Asp Asn Glu Ser His Trp Gln Ala

290 295 300

Leu Ala Asp Gly Thr Ile Asp Phe Val Gly Thr Asp His Ala Pro Tyr

305 310 315 320

Glu Ile Ala Thr Glu Lys Asp Tyr Pro Gly Ala Thr Ile Trp Asn Thr

325 330 335

Phe Pro Gly Ile Pro Gly Val Glu Thr Met Val Pro Ile Leu Val Ser

340 345 350

Glu Gly Leu Asn Lys Gly Arg Leu Ser Leu Ser Arg Phe Val Glu Val

355 360 365

Thr Ser Arg Asn Ala Ala Ile His Tyr Gly Ile Tyr Pro Lys Lys Gly

370 375 380

Ser Met Glu Ile Gly Ser Asp Ala Asp Phe Thr Val Ile Asp Leu Glu

385 390 395 400

Lys Glu Tyr Val Ile Asp Glu Gln Lys Thr Glu Ser Met Ala Lys Tyr

405 410 415

Asn Pro Leu His Gly Met Lys Leu Lys Gly Lys Pro Val Gln Thr Ile

420 425 430

Ile Arg Gly Asn Val Val Phe Glu Glu Asp Lys Gly Ile Val Gly Glu

435 440 445

Ala Gly Tyr Gly Glu Tyr Val Pro Arg Gln Ser Ile Gln Arg Leu Glu

450 455 460

Arg Thr Phe Lys Tyr Glu Lys Tyr Glu Ala Val Asn Val Lys Thr Lys

465 470 475 480

Asp Pro Val Ala Gln Gln

485

<210> 19

<211> 512

<212> PRT

<213> 产氢氨基酸杆菌(Acidaminobacter hydrogenoformans)

<400> 19

Met Lys Tyr Asp Leu Ile Val Lys Asn Ala Leu Ile Pro Ala Met Asn

1 5 10 15

Gly Asn Asp Thr Leu Met Thr Asn Ile Leu Val Lys Asn Glu Lys Ile

20 25 30

Ala Gly Phe Leu Glu Ser Val Glu Gly Leu Glu Ala Glu Arg Tyr Val

35 40 45

Asp Ala Gly Gly Lys Leu Val Leu Pro Gly Cys Ile Asp Ser His Thr

50 55 60

His Tyr Met Asp Pro Gly Phe Thr His Arg Glu Thr Phe Leu Thr Gly

65 70 75 80

Thr Ser Gln Ala Ala Ala Gly Gly Leu Thr Thr Ile Ile Asp Met Pro

85 90 95

Cys Cys Ser Phe Pro Arg Ser Val Leu Asp Val Asp Ser Met Asn Glu

100 105 110

Lys Leu Asp Ala Ile Lys Pro Gln Ala Ile Val Asp Phe Gly Leu Trp

115 120 125

Gly Gly Ala Thr Gly Asp Met Val Arg Asp Gly Lys Leu Ala Asp Val

130 135 140

Gln Ala Gln Ala Asp Ala Gly Val Leu Ala Phe Lys Val Tyr Met Thr

145 150 155 160

Pro Ser Val Pro Thr Phe Asn Arg Val Thr Asp Pro Glu Met His Glu

165 170 175

Val Phe Lys Ala Val Glu Lys Thr Gly Val Ala Val Gly Val His Ala

180 185 190

Glu Asn Phe Ala Met Cys Asp Tyr Tyr Val Arg Lys Tyr Gln Ala Glu

195 200 205

Gly Arg Leu Asp Gly Pro Ala Trp Ser Glu Ala Arg Leu Glu Leu Ala

210 215 220

Glu Lys Ala Ala Ile Gln Leu Gly Ile Ala Phe Ala Glu Glu Thr Gly

225 230 235 240

Ala Arg Tyr His Val Val His Met Ser Thr Gly Met Gly Ala Arg Leu

245 250 255

Ile Gly Glu Ala Lys Lys Arg Gly Leu Lys Val Thr Ser Glu Val Cys

260 265 270

Pro His Tyr Leu Val Leu Asn Ala Pro Glu Ala Met Ser Glu Tyr Gly

275 280 285

Ala Tyr Ala Lys Ile Ala Pro Pro Leu Arg Thr Thr Gln Asp Asn Ile

290 295 300

Glu Leu Trp Glu Gly Leu Ala Asn Gly Ser Val Asp Phe Val Ala Thr

305 310 315 320

Asp His Ala Pro Tyr Lys Ile Gln Ala Asn Lys Asp Asn Asp Asp Ala

325 330 335

Ala Val Glu Lys Asp Ala Pro Gly Met Asn Ile Trp Thr Ala Phe Pro

340 345 350

Gly Ile Ser Gly Ser Glu Thr Met Val Asn Ile Ile Ile Ser Glu Gly

355 360 365

Tyr Asn Lys Gly Arg Leu Thr Leu Glu Arg Thr Val Glu Val Leu Ser

370 375 380

Lys Ser Ala Ala Ile His Tyr Gly Leu Tyr Pro Lys Lys Gly Ser Met

385 390 395 400

Asn Ile Gly Ser Asp Ala Asp Leu Thr Ile Ile Asp Leu Glu Lys Glu

405 410 415

Trp Ala Ile Asp Lys Asp Val Ser Phe Met Lys Asn Lys Tyr Thr Pro

420 425 430

Leu His Gly Leu Lys Leu Lys Gly Lys Val Ala His Thr Ile Leu Arg

435 440 445

Gly Thr Ser Val Tyr His Glu Glu Pro Asn Glu Val Gln Gly Lys Ile

450 455 460

Leu Val Glu Pro Gly Phe Gly Gln Phe Val Lys Arg Gln Ser Ile Gln

465 470 475 480

Val Leu Pro Asn Lys Leu Gln Phe Glu Asn Tyr Lys Ala Ile Gly Ala

485 490 495

Asp Phe Phe Asn Asn Tyr His Arg Arg Asn Ile Asn Asn Val Ile Asp

500 505 510

<210> 20

<211> 470

<212> PRT

<213> 温铁还原细菌(Caloranaerobacter sp.)

<400> 20

Met Tyr Asp Val Ile Ile Lys Asn Ala Arg Ile Pro Gln Gly Asp Asp

1 5 10 15

Thr Ile Leu Thr Asn Ile Leu Val Lys Asp Glu Lys Ile Ala Gly Phe

20 25 30

Thr Gln Asp Leu Asp Gly Ile Asp Ala Lys Glu Ile Ile Asp Ala Glu

35 40 45

Gly His Leu Thr Leu Pro Gly Cys Ile Asp Ser His Thr His Ile Asn

50 55 60

Asp Pro Gly Phe Thr His Arg Glu Asn Phe Leu Thr Gly Thr Ser Ala

65 70 75 80

Ala Ala Ser Gly Gly Ile Thr Thr Ile Ile Asp Met Pro Cys Cys Ser

85 90 95

Val Pro Ser Val Arg Ser Val Glu Asn Leu Glu His Lys Leu Glu Ala

100 105 110

Ile Lys Asp Lys Ala Ile Val Asp Tyr Ala Met Trp Gly Gly Val Thr

115 120 125

Gly Glu Asp Val Arg Asn Gly Trp Leu His Asn Val Glu Glu Gln Ala

130 135 140

Asp Tyr Gly Val Cys Ala Phe Lys Val Tyr Met Thr Pro Ser Val Pro

145 150 155 160

Thr Tyr Pro Arg Val Thr Asp Pro Glu Met Leu Glu Ala Phe Lys Ala

165 170 175

Val Ala Lys Thr Gly Leu Pro Ile Gly Ile His Ala Glu Asn Phe Ala

180 185 190

Met Cys Asp Phe Tyr Val Lys Lys Phe Gln Ala Glu Gly Arg Met Asp

195 200 205

Gly Pro Ala Trp Ala Glu Ala Arg Met Thr Leu Ala Glu Lys Val Ala

210 215 220

Ile Gln Leu Gly Ile Ser Phe Ala Glu Glu Thr Gly Ala Arg Leu His

225 230 235 240

Ile Val His Met Ser Thr Gly Ile Gly Ala Ile Leu Val Gly Glu Ala

245 250 255

Lys Lys Arg Gly Leu Asp Val Thr Ala Glu Thr Cys Pro His Tyr Leu

260 265 270

Thr Leu Asn Tyr Lys Asp Ala Met Ser Glu Phe Lys Gln Phe Ala Lys

275 280 285

Ile Ala Pro Pro Leu Arg Thr Lys Glu Asp Asn Glu Ile Leu Trp Lys

290 295 300

Ala Leu Gln Asp Gly Arg Val Asp Phe Ile Ala Thr Asp His Ala Pro

305 310 315 320

Tyr Glu Ile Pro Thr Glu Lys Glu Ala Glu Gly Met Asn Ile Trp Thr

325 330 335

Ala Phe Pro Gly Ile Pro Gly Val Glu Thr Met Val Pro Val Ile Ile

340 345 350

Ser Glu Gly Tyr Asn Lys Gly Arg Leu Ser Leu Ser Arg Leu Val Glu

355 360 365

Val Leu Ser Thr Asn Pro Ala Lys His Tyr Gly Leu Tyr Pro Lys Lys

370 375 380

Gly Ala Met Phe Ile Gly Ser Asp Ala Asp Phe Thr Ile Ile Asp Leu

385 390 395 400

Glu Lys Glu Trp Thr Ile Asp Lys Asp Lys Met Tyr Thr Met Ala Lys

405 410 415

Tyr Thr Pro Phe His Gly Phe Lys Leu Lys Gly Lys Pro Val Lys Thr

420 425 430

Val Val Arg Gly Lys Leu Val Tyr Asp Asp Thr Glu Gly Ile Val Gly

435 440 445

Arg Glu Gly Tyr Gly Lys Phe Ile Lys Arg Gln Asn Lys Gly Lys Leu

450 455 460

Glu Arg Leu Ile Lys Phe

465 470

<210> 21

<211> 532

<212> PRT

<213> 组织菌(Tissierella sp.)

<400> 21

Met Tyr Asp Ile Ile Val Lys Asn Ala Arg Ile Pro Gln Gly Asp Asp

1 5 10 15

Thr Ile Ile Thr Asn Ile Leu Val Lys Asp Glu Lys Ile Ala Gly Phe

20 25 30

Leu Asp Asn Ile Glu Gly Ile Ala Ala Lys Glu Ile Ile Asp Ala Glu

35 40 45

Gly His Leu Thr Leu Pro Gly Cys Ile Asp Ser His Thr His Phe Met

50 55 60

Tyr Gln Gly Phe Pro His Arg Glu Asn Phe Leu Thr Gly Thr Ala Ala

65 70 75 80

Ala Ala Ser Gly Gly Val Thr Thr Ile Ile Asp Met Pro Cys Cys Ser

85 90 95

Val Pro Ser Val Arg Ser Ala Lys Gln Leu Gln Leu Lys Val Asp Leu

100 105 110

Ile Gln Pro Gln Ala Leu Val Asp Phe Ala Leu Trp Gly Gly Val Thr

115 120 125

Gly Glu Asp Val Arg Glu Gly Trp Leu Asp His Val Gln Glu Gln Ala

130 135 140

Asp Tyr Gly Val Val Ala Phe Lys Ala Tyr Met Thr Pro Ser Val Pro

145 150 155 160

Thr Phe Pro Arg Val Thr Asp Pro Glu Met Tyr Glu Ala Phe Lys Ala

165 170 175

Val Ala Lys Thr Gly Leu Pro Val Gly Ile His Ala Glu Asn Tyr Ser

180 185 190

Met Cys Asp Tyr Tyr Val Lys Glu Phe Gln Lys Gln Gly Arg Met Asp

195 200 205

Gly Pro Ala Trp Ala Glu Ala Arg Met Glu Leu Ala Glu Lys Val Ala

210 215 220

Ile Glu Leu Gly Ile Ser Phe Ala Glu Asp Ala Gly Ala Arg Leu His

225 230 235 240

Ile Val His Met Ser Thr Gly Ile Gly Ala Lys Leu Val Gly Glu Ala

245 250 255

Lys Lys Arg Gly Leu Asn Val Thr Ser Glu Ser Cys Pro His Tyr Leu

260 265 270

Thr Leu Asn Tyr Gln Glu Ser Met Thr Lys Tyr Gly Ala Phe Ala Lys

275 280 285

Ile Ala Pro Pro Leu Arg Thr Lys Lys Asp Asn Glu Glu Gln Trp Glu

290 295 300

Gly Ile Asn Asn Gly Ser Ile Asp Phe Ile Ala Thr Asp His Ala Pro

305 310 315 320

Tyr Glu Ile Glu Ser Glu Lys Ala Arg Glu Gly Met Asn Ile Trp Thr

325 330 335

Ala Phe Pro Gly Ile Pro Gly Val Glu Thr Met Val Pro Ile Ile Val

340 345 350

Ser Glu Gly Tyr Asn Lys Gly Arg Ile Ser Leu Ser Lys Leu Val Glu

355 360 365

Ile Leu Ser Thr Asn Ala Ala Ile His Tyr Gly Leu Tyr Pro Lys Lys

370 375 380

Gly Ala Met His Ile Gly Ser Asp Ala Asp Phe Thr Ile Ile Asp Leu

385 390 395 400

Asp Lys Glu Trp Val Ile Glu Pro Lys Lys Gln Ala Ser Met Cys Gly

405 410 415

Tyr Ser Pro Leu Glu Gly Met Lys Leu Lys Gly Lys Val Ala Lys Thr

420 425 430

Ile Val Arg Gly His Leu Val Phe Glu Asp Ile Glu Glu Ser Val Leu

435 440 445

Ala Gly Leu Thr Asp Glu Glu Leu Lys Thr Ile Val His Asn Phe Pro

450 455 460

Glu Gly Ile Asp Glu Lys Tyr Ala Asp Glu Phe Ser Lys Tyr Pro Tyr

465 470 475 480

Leu His Ser Ala Glu Tyr Glu Asn Ser Tyr Arg Ile Asn His Pro Glu

485 490 495

Val Ile Asp Lys His Ile Arg Glu Ile Lys Gly Ile Lys Ala Lys Pro

500 505 510

Gly Phe Gly Gln Phe Ile Lys Arg Gln Ser Ile Gln Ile Leu Pro Arg

515 520 525

Lys Ile Thr Phe

530

<210> 22

<211> 497

<212> PRT

<213> 芽孢杆菌(Bacillus sp. )

<400> 22

Met Asp Leu Ile Leu Lys Asn Ala Leu Ile Pro Gln Gly Asp Arg Lys

1 5 10 15

Val Lys Thr Asn Ile Leu Val Asp Asp Gly Lys Ile Val Gly Phe Thr

20 25 30

Ser Ala Leu Asn Gly Leu Ser Ala Asp Arg Val Ile Asp Met Lys Glu

35 40 45

Asn Leu Val Val Pro Gly Cys Ile Asp Pro His Thr His Phe Met Asp

50 55 60

Pro Gly Phe Thr His Arg Glu Thr Phe Thr Thr Gly Thr Arg Ser Ala

65 70 75 80

Ala Ala Gly Gly Val Thr Thr His Ile Asp Met Pro Cys Cys Ser Lys

85 90 95

Pro Ser Val Arg Asp Ala Glu Ser Phe His Lys Lys Leu Ala Pro Leu

100 105 110

Lys Asp Gln Ala Tyr Ile Asp Phe Ala Phe Trp Gly Gly Met Thr Gly

115 120 125

Glu Asp Val Arg Glu Gly Leu Leu Asp Asn Ile Gln Pro Gln Ile Asp

130 135 140

Glu Gly Val Val Ala Phe Lys Val Tyr Met Thr Pro Ser Val Pro Thr

145 150 155 160

Phe Pro Lys Val Ser Asp Ala Glu Met Leu Glu Ile Phe Ser His Ile

165 170 175

Ala Pro Thr Gly Met Pro Ile Gly Val His Ala Glu Asn Tyr Asp Ile

180 185 190

Cys Thr Phe Tyr Ser Glu Lys Leu Lys Lys Glu Gly Arg Leu Asp Gly

195 200 205

Pro Ala Trp Ala Glu Ala Arg Met Ala Leu Ala Glu Lys Ala Ala Ile

210 215 220

Gln Leu Ile Ile Ser Phe Ala Glu Ala Thr Gly Ala Arg Val His Val

225 230 235 240

Val His Met Ser Thr Lys Glu Gly Val Glu Leu Val Arg Ala Ala Lys

245 250 255

Lys Arg Gly Val Lys Val Thr Ala Glu Thr Cys Pro His Tyr Leu Met

260 265 270

Leu Asn Ala Glu Asp Ser Met Ser Glu Arg Gly Thr Phe Ala Lys Ile

275 280 285

Ala Pro Pro Leu Arg Glu Lys Glu Asp Asn Ile Val His Trp Gln Ala

290 295 300

Leu Ala Asp Gly Thr Ile Asp Phe Val Gly Thr Asp His Ala Pro Tyr

305 310 315 320

Glu Ile Pro Thr Glu Lys Asp Ala Pro Asp Ser Thr Ile Trp Asn Ser

325 330 335

Phe Pro Gly Ile Pro Gly Val Glu Thr Met Val Pro Ile Leu Val Ser

340 345 350

Glu Gly Leu Asn Lys Gly Arg Leu Ser Leu Ser Arg Phe Val Glu Val

355 360 365

Thr Ser Arg Asn Ala Ala Ile His Phe Gly Ile Tyr Pro Lys Lys Gly

370 375 380

Ala Met Glu Ile Gly Ser Asp Ala Asp Phe Thr Val Ile Asp Leu Glu

385 390 395 400

Arg Glu Tyr Thr Ile Asp Glu Gln Lys Thr Glu Ser Met Ala Lys Tyr

405 410 415

Asn Pro Leu His Gly Leu Lys Leu Lys Gly Lys Pro Ile Gln Thr Ile

420 425 430

Val Arg Gly Arg Val Val Tyr Asp Glu Asp Asn Gly Gly Ile Met Gly

435 440 445

Glu Ala Gly Tyr Gly Gln Tyr Val Lys Arg Gln Ser Ile Gln Ser Leu

450 455 460

Glu Arg Val Ile Lys Tyr Glu Thr Phe Glu Glu Gly Ala Lys Glu Ala

465 470 475 480

Glu Ala Lys Pro Arg Met Glu Lys Ala Leu Lys Arg Asp Met Ile Thr

485 490 495

Asn

<210> 23

<211> 470

<212> PRT

<213> Paenibacillus donghaensis

<400> 23

Met Ser Tyr Glu Leu Val Ile Arg Asn Ala Ser Ile Pro Gln Gly Asp

1 5 10 15

Arg Gln Val Leu Thr Asp Ile Leu Val Asp Lys Gly Val Ile Val Gly

20 25 30

Phe Ala Arg Asp Phe Glu Pro Gly Asp Ala Glu Val Ile Asp Ala Lys

35 40 45

Gly Met Leu Val Leu Pro Gly Val Ile Asp Ser His Thr His Phe Asn

50 55 60

Asp Pro Gly Phe Thr His Arg Glu Asp Phe Val Thr Gly Thr Ser Ser

65 70 75 80

Ala Ala Ala Gly Gly Val Thr Met Ile Val Asp Met Pro Cys Cys Ser

85 90 95

Val Pro Ser Val Arg Ser Val Asp Asn Leu His Ser Lys Leu Asn Ala

100 105 110

Leu Glu Gly Lys Gly Ile Ile Asp Tyr Ala Met Trp Gly Gly Val Thr

115 120 125

Gly Glu Asp Val Arg Asn Asp Val Leu Asn Ile Val Lys Glu Gln Ala

130 135 140

Ala Glu Gly Val Val Ala Phe Lys Val Tyr Met Thr Pro Ser Val Pro

145 150 155 160

Thr Tyr Pro Arg Val Thr Asp Pro Glu Met Leu Glu Ala Phe Tyr Ala

165 170 175

Val Ala Glu Thr Gly Met Pro Ile Gly Ile His Ala Glu Asn Phe Ala

180 185 190

Ile Cys Asp Phe Tyr Thr Asn Lys Leu Lys Lys Glu Gly Arg Thr Asp

195 200 205

Tyr Pro Ala Trp Ala Glu Ala Arg Lys Ile Leu Ala Glu Lys Ala Ala

210 215 220

Ile Gln Leu Gly Ile Ser Phe Ser Glu Glu Ser Gly Ala Arg Leu His

225 230 235 240

Ile Val His Met Ser Thr Ala Ala Gly Asn Gly Leu Ile Lys Ala Ala

245 250 255

Lys Leu Lys Gly Val Lys Val Thr Ala Glu Thr Cys Pro His Tyr Leu

260 265 270

Thr Leu Asn Ala Val Asp Ala Met Thr Glu Phe Glu Ala Phe Ala Lys

275 280 285

Ile Ala Pro Pro Leu Arg Arg Pro Glu Asp Asn Glu Ser Leu Trp Gln

290 295 300

Gly Leu Val Asp Gly Thr Ile Asp Phe Met Ala Thr Asp His Ala Pro

305 310 315 320

Tyr Glu Ile Ala Thr Glu Lys Asp Ala Pro Gly Met Asp Val Trp Thr

325 330 335

Ser Phe Pro Gly Ile Pro Gly Val Glu Thr Leu Val Pro Ile Met Ile

340 345 350

Ser Glu Gly Tyr Asn Lys Gly Arg Leu Ser Leu Ser Arg Leu Val Glu

355 360 365

Leu Leu Cys Thr Asn Pro Ala Val His Tyr Gly Leu Tyr Pro Lys Lys

370 375 380

Gly Ala Ile Glu Ile Gly Thr Asp Ala Asp Phe Thr Ile Val Asp Leu

385 390 395 400

Glu Lys Glu Trp Thr Leu Asp Lys Asp Gln Met His Cys Lys Pro Lys

405 410 415

Tyr Thr Pro Phe His Gly Met Lys Leu Lys Gly Lys Val Asp Lys Thr

420 425 430

Ile Val Arg Gly Thr Val Val Tyr Asp Asp Glu Leu Gly Val Val Gly

435 440 445

Lys Pro Gly Tyr Gly Glu Phe Val Lys Arg Gln Thr Ile Ser Glu Leu

450 455 460

Pro Arg Leu Leu Lys Tyr

465 470

<210> 24

<211> 555

<212> PRT

<213> Gottschalkia acidurici

<400> 24

Met Ser Tyr Asp Val Ile Ile Lys Asn Ala Arg Ile Pro Gln Gly Asp

1 5 10 15

Asn Thr Val Leu Thr Asn Ile Leu Val Lys Asp Glu Lys Ile Ala Gly

20 25 30

Phe Ile Asn Cys Ile Asp Gly Ile Glu Ala Lys Asn Ile Ile Asp Ala

35 40 45

Glu Gly Asn Leu Thr Ala Pro Gly Phe Ile Asp Ser His Thr His Tyr

50 55 60

Met Tyr Gln Gly Phe Pro His Arg Glu Asn Phe Leu Thr Gly Ser Ala

65 70 75 80

Ala Ser Ala Arg Gly Gly Ile Thr Thr Val Val Asp Met Pro Cys Cys

85 90 95

Ser Val Pro Ser Ala Arg Ser Val Asp Gln Leu Lys Leu Lys Ile Asp

100 105 110

Val Cys Ala Pro Gln Ala Val Ile Asp Tyr Ala Met Trp Gly Gly Val

115 120 125

Thr Gly Glu Asp Val Arg Glu Gly Trp Leu His Asn Val Lys Glu Gln

130 135 140

Ala Asp Tyr Gly Val Val Gly Phe Lys Val Tyr Met Thr Pro Ser Val

145 150 155 160

Pro Thr Tyr Pro Arg Val Thr Asp Pro Glu Met Leu Glu Ile Phe Lys

165 170 175

Ala Val Ala Lys Thr Gly Leu Pro Val Gly Val His Ala Glu Asn Phe

180 185 190

Ala Ile Cys Asp Tyr Tyr Val Lys Lys Phe Gln Glu Glu Gly Arg Met

195 200 205

Asp Ala Pro Ala Trp Ser Glu Ala Arg Asn Glu Leu Ala Glu Lys Val

210 215 220

Ala Ile Glu Leu Gly Ile Ser Phe Ala Glu Ala Thr Gly Ala Arg Tyr

225 230 235 240

His Val Val His Met Ser Thr Gly Ile Gly Ala Lys Leu Val Ala Asp

245 250 255

Ala Lys Arg Arg Gly Ile Asn Val Thr Ala Glu Ser Cys Pro His Tyr

260 265 270

Leu Thr Leu Asn Tyr Gln Asp Thr Leu Thr Lys Phe Gly Ser Phe Ala

275 280 285

Lys Val Ala Pro Pro Leu Arg Thr Ser Ala Asp Ile Glu Glu Gln Trp

290 295 300

Glu Gly Leu Arg Asp Gly Ser Ile Asp Phe Met Ala Thr Asp His Ala

305 310 315 320

Pro Tyr Glu Ile Glu Ser Glu Lys Lys Lys Glu Gly Met Asn Ile Trp

325 330 335

Thr Ala Phe Pro Gly Phe Pro Gly Thr Glu Thr Met Ala Pro Ile Ile

340 345 350

Ile Ser Glu Gly Leu Asn Lys Gly Arg Leu Ser Leu Ser Arg Thr Ile

355 360 365

Glu Val Leu Ser Thr Ser Ala Ala Lys Gln Tyr Gly Leu Tyr Pro Lys

370 375 380

Lys Gly Ala Leu Glu Ile Gly Ser Asp Ala Asp Phe Ala Ile Val Asp

385 390 395 400

Leu Asp Lys Glu Trp Val Ile Asp Ser Lys Glu Asn Gln Ala Ser Met

405 410 415

Cys Gly Tyr Thr Pro Leu Glu Gly Met Lys Leu Lys Gly Arg Ile Val

420 425 430

Lys Thr Ile Val Arg Gly Asn Ile Val Phe Asp Asp Thr Gln Glu Gly

435 440 445

Val Leu Pro Ser Leu Thr Asp Glu Glu Met Lys Thr Ile Val His Asn

450 455 460

Tyr Pro Ala Gly Val Glu Glu Asp Tyr Ala Asp Ile Phe Glu Gln Phe

465 470 475 480

Pro Asn Leu His Ser Ala Glu Tyr Asp Arg Ser Tyr Arg Lys Glu His

485 490 495

Pro Glu Lys Ile Asp Glu Asp Val Arg Arg Ile Lys Gly Ile Met Val

500 505 510

Lys Pro Gly Phe Gly Gln Leu Val Lys Arg Gln Ser Ile Gln Glu Leu

515 520 525

Pro Lys Thr Leu Thr Tyr Asp Val Lys Ala Pro Thr Gly Ile Asn Arg

530 535 540

Arg Glu Arg Glu Ser Leu Pro Glu Phe Asn Lys

545 550 555

<210> 25

<211> 497

<212> PRT

<213> 强壮芽孢杆菌(Bacillus fortis)

<400> 25

Met Asp Leu Ile Leu Lys Asn Ala Leu Ile Pro Gln Gly Asp Arg Lys

1 5 10 15

Val Lys Thr Asn Ile Leu Val Asp Asp Gly Lys Ile Val Gly Phe Thr

20 25 30

Ser Ala Leu Asn Gly Phe Ser Ala Asp Arg Val Ile Asp Met Lys Glu

35 40 45

Asn Leu Val Val Pro Gly Cys Ile Asp Pro His Thr His Phe Met Asp

50 55 60

Pro Gly Phe Thr His Arg Glu Thr Phe Thr Thr Gly Thr Arg Ser Ala

65 70 75 80

Ala Ala Gly Gly Val Thr Thr His Ile Asp Met Pro Cys Cys Ser Lys

85 90 95

Pro Ser Val Arg Asp Ala Glu Ser Phe His Lys Lys Leu Ala Pro Leu

100 105 110

Lys Asp Gln Ala Tyr Ile Asp Phe Ala Phe Trp Gly Gly Met Thr Gly

115 120 125

Glu Asp Val Arg Glu Gly Leu Leu Asp Asn Ile His Pro Gln Ile Asp

130 135 140

Glu Gly Val Val Ala Phe Lys Val Tyr Met Thr Pro Ser Val Pro Thr

145 150 155 160

Phe Pro Lys Val Ser Asp Ala Glu Met Leu Glu Ile Phe Ser His Ile

165 170 175

Ala Pro Thr Gly Met Pro Ile Gly Val His Ala Glu Asn Tyr Asp Ile

180 185 190

Cys Thr Phe Tyr Ser Glu Lys Leu Lys Lys Glu Gly Arg Leu Asp Gly

195 200 205

Pro Ala Trp Ala Glu Ala Arg Met Ala Leu Ala Glu Lys Ala Ala Ile

210 215 220

Gln Leu Ile Ile Ser Phe Ala Glu Ala Thr Gly Ala Arg Val His Val

225 230 235 240

Val His Met Ser Thr Lys Glu Gly Val Glu Leu Val Arg Glu Ala Lys

245 250 255

Lys Arg Gly Val Lys Val Thr Ala Glu Thr Cys Pro His Tyr Leu Met

260 265 270

Leu Asn Ala Glu Asp Ser Met Ser Glu Arg Gly Thr Phe Ala Lys Ile

275 280 285

Ala Pro Pro Leu Arg Glu Lys Asp Asp Asn Ile Val His Trp Gln Ala

290 295 300

Leu Ala Asp Gly Thr Ile Asp Phe Val Gly Thr Asp His Ala Pro Tyr

305 310 315 320

Glu Ile Ala Thr Glu Lys Asp Ala Pro Asp Ser Thr Ile Trp Asn Ser

325 330 335

Phe Pro Gly Ile Pro Gly Val Glu Thr Met Val Pro Ile Leu Val Ser

340 345 350

Glu Gly Leu Asn Lys Gly Arg Leu Ser Leu Ser Arg Phe Val Glu Val

355 360 365

Thr Ser Arg Asn Ala Ala Ile His Phe Gly Ile Tyr Pro Lys Lys Gly

370 375 380

Ala Met Glu Ile Gly Ser Asp Ala Asp Phe Thr Val Ile Asp Leu Glu

385 390 395 400

Arg Glu Tyr Thr Ile Asp Glu Gln Lys Thr Glu Ser Met Ala Lys Tyr

405 410 415

Asn Pro Leu His Gly Leu Lys Leu Lys Gly Lys Pro Ile Gln Thr Ile

420 425 430

Val Arg Gly Arg Val Val Tyr Asp Glu Asp Asn Gly Gly Ile Val Gly

435 440 445

Asp Ala Gly Tyr Gly Gln Phe Val Lys Arg Gln Ser Ile Gln Ser Leu

450 455 460

Glu Arg Val Ile Lys Tyr Glu Thr Tyr Glu Glu Gly Ala Lys Glu Ala

465 470 475 480

Glu Ala Lys Pro Arg Val Glu Lys Ala Leu Arg Arg Asp Leu Ile Thr

485 490 495

Asn

<210> 26

<211> 485

<212> PRT

<213> 海泥芽孢杆菌(Bacillus oceanisediminis)

<400> 26

Met Asp Leu Ile Leu Lys Asn Ala Asn Ile Pro Gln Gly Asp Arg Gln

1 5 10 15

Val Leu Thr Asn Ile Leu Val Asn Asp Gly Lys Ile Val Gly Tyr Thr

20 25 30

Asn Asp Ile Ser Phe Leu Asn Ala Lys Lys Val Ile Asp Ile Ala Gly

35 40 45

Lys Leu Val Val Pro Gly Cys Ile Asp Pro His Thr His Phe Met Asp

50 55 60

Pro Gly Phe Thr His Arg Glu Thr Phe Ala Thr Gly Ser Arg Ser Ala

65 70 75 80

Ala Ala Gly Gly Leu Thr Thr Ile Ile Asp Met Pro Cys Cys Ser Lys

85 90 95

Pro Ser Val Arg Asp Gly Glu Ser Phe Asn Lys Lys Ile Ala Pro Ile

100 105 110

Arg Gly Gln Ala Tyr Ile Asp Tyr Cys Phe Trp Gly Gly Met Thr Gly

115 120 125

Glu Asp Ala Arg Glu Gly Trp Ile Asp Asn Ile Tyr Pro Gln Ile Asp

130 135 140

Gln Gly Val Val Ala Phe Lys Val Tyr Met Thr Pro Ser Val Pro Thr

145 150 155 160

Phe Pro Lys Val Ser Asp Ala Glu Met Leu Glu Ile Phe Ser Asn Val

165 170 175

Ala Lys Thr Gly Leu Pro Ile Gly Val His Ala Glu Asn Tyr Asp Ile

180 185 190

Cys Thr Phe Tyr Ser Lys Lys Leu Glu Lys Glu Gly Arg Met Asp Gly

195 200 205

Pro Ala Trp Ala Glu Ala Arg Ser Ser Leu Ala Glu Lys Val Ala Ile

210 215 220

Glu Leu Ile Leu Ser Phe Ala Glu Ala Thr Asp Ala Arg Val His Val

225 230 235 240

Val His Met Ser Thr Lys Glu Gly Val Glu Leu Val Lys Ala Ala Lys

245 250 255

Lys Arg Gly Val Lys Val Thr Ala Glu Thr Cys Pro His Tyr Leu Val

260 265 270

Leu Asn Ala Lys Asp Ala Met Thr Glu Arg Gly Ala Tyr Ala Lys Ile

275 280 285

Ala Pro Pro Leu Arg Gly Lys Glu Asp Asn Glu Ser His Trp Gln Ala

290 295 300

Leu Ala Asp Gly Thr Ile Asp Phe Val Gly Thr Asp His Ala Pro Tyr

305 310 315 320

Glu Ile Ala Thr Glu Lys Asp Tyr Pro Gly Ala Thr Ile Trp Asn Thr

325 330 335

Phe Pro Gly Ile Pro Gly Val Glu Thr Met Val Pro Ile Leu Val Ser

340 345 350

Glu Gly Leu Asn Lys Gly Arg Leu Ser Leu Ser Arg Phe Val Glu Val

355 360 365

Thr Ser Arg Asn Ala Ala Ile His Tyr Gly Ile Tyr Pro Lys Lys Gly

370 375 380

Ser Met Glu Ile Gly Ala Asp Ala Asp Phe Thr Val Ile Asp Leu Asn

385 390 395 400

Lys Glu Tyr Val Ile Asp Glu Gln Lys Thr Glu Ser Met Ala Lys Tyr

405 410 415

Asn Pro Leu His Gly Met Lys Leu Lys Gly Lys Pro Val Gln Thr Ile

420 425 430

Val Arg Gly Asn Ile Val Phe Glu Glu Asp Lys Gly Ile Thr Gly Glu

435 440 445

Ala Gly Tyr Gly Glu Phe Val Pro Arg Gln Ser Ile Gln Arg Leu Glu

450 455 460

Arg Thr Phe Lys Tyr Glu Lys Tyr Asn Glu Val Lys Val Thr Ser Lys

465 470 475 480

Glu Thr Val Lys Gln

485

<210> 27

<211> 493

<212> PRT

<213> Virgibacillus profundi

<400> 27

Met Asp Leu Ile Leu Lys Asn Ala Leu Ile Pro Gln Gly Asp Arg Lys

1 5 10 15

Val Glu Thr Asn Ile Leu Val Asp Asn Gly Glu Ile Val Gly Phe Thr

20 25 30

Asn Ala Ile Asn Gly Met Ala Ala Lys Glu Ile Ile Asp Ile Lys Gly

35 40 45

Asn Leu Val Val Pro Gly Cys Ile Asp Pro His Thr His Phe Met Asp

50 55 60

Pro Gly Phe Thr His Arg Glu Thr Phe Ala Thr Gly Thr Arg Ser Ala

65 70 75 80

Ala Ala Gly Gly Ile Thr Thr His Ile Asp Met Pro Cys Cys Ser Lys

85 90 95

Pro Ser Val Arg Asp Arg Glu Ser Phe Tyr Lys Lys Ile Glu Pro Leu

100 105 110

Lys Ser Gln Ala Tyr Ile Asp Tyr Cys Phe Trp Gly Gly Met Thr Gly

115 120 125

Glu Asp Val Arg Glu Glu Leu Leu Asp Asn Ile Tyr Pro Gln Ile Glu

130 135 140

Glu Gly Val Val Ala Phe Lys Val Tyr Met Thr Pro Ser Val Pro Thr

145 150 155 160

Phe Pro Lys Val Thr Asp Ala Glu Met Leu Glu Ile Phe Ser Lys Ile

165 170 175

Ala Pro Thr Gly Met Pro Ile Gly Val His Ala Glu Asn Tyr Glu Ile

180 185 190

Cys Thr Phe Tyr Ser Glu Lys Met Lys Lys Glu Gly Arg Met Asp Gly

195 200 205

Pro Ala Trp Ala Glu Ala Arg Met Ala Leu Ala Glu Lys Ala Ala Ile

210 215 220

Gln Leu Val Ile Ser Phe Ala Glu Ala Thr Gly Ala Arg Val His Ile

225 230 235 240

Val His Met Thr Thr Lys Glu Gly Val Glu Leu Val Arg Glu Ala Lys

245 250 255

Lys Arg Gly Val Lys Val Thr Ala Glu Thr Cys Pro His Tyr Leu Val

260 265 270

Leu Asn Ala Gln Asp Ser Met Thr Glu Arg Gly Thr Phe Ala Lys Ile

275 280 285

Ala Pro Pro Leu Arg Glu Lys Glu Asp Asn Ile Val His Trp Gln Gly

290 295 300

Leu Ala Asp Gly Ser Ile Asp Phe Val Gly Thr Asp His Ala Pro Tyr

305 310 315 320

Glu Ile Ala Thr Glu Lys Asp Ala Pro Asp Ser Asp Ile Trp Asn Ser

325 330 335

Phe Pro Gly Ile Pro Gly Val Glu Thr Met Val Pro Ile Leu Val Ser

340 345 350

Glu Gly Leu Asn Lys Gly Arg Leu Ser Leu Ser Arg Phe Val Glu Val

355 360 365

Thr Ser Arg Asn Ala Ala Ile His Tyr Gly Ile Tyr Pro Lys Lys Gly

370 375 380

Ser Met Glu Leu Gly Ser Asp Ala Asp Phe Thr Val Ile Asp Leu Glu

385 390 395 400

Arg Glu Tyr Thr Ile Asp Glu Gln Lys Thr Glu Ser Met Ala Lys Tyr

405 410 415

Asn Pro Leu His Gly Met Gln Leu Lys Gly Met Pro Ile Gln Thr Ile

420 425 430

Val Arg Gly Lys Val Val Tyr Asp Glu Asp Asn Gly Gly Ile Leu Gly

435 440 445

Glu Ser Gly Tyr Gly Glu Phe Val Lys Arg Gln Ser Ile Gln Lys Leu

450 455 460

Asp Arg Ser Ile Lys Tyr Glu Val Tyr Glu Glu Thr Ala Lys Glu Leu

465 470 475 480

Ala Ser Gln Gln Arg Gln Glu Lys Ala Leu Ile Asn Asn

485 490

<210> 28

<211> 471

<212> PRT

<213> 类芽孢杆菌(Paenibacillus sp. )

<400> 28

Met Ser Tyr Asp Leu Ile Ile Lys Asn Ala Ser Ile Pro Gln Gly Asp

1 5 10 15

Arg Gln Val Leu Thr Asp Ile Leu Val Asn Asn Gly Val Ile Val Gly

20 25 30

Phe Ala Lys Asp Phe Glu Gly Asp Ala Ala Glu Ile Ile Asp Ala Gly

35 40 45

Gly Lys Leu Val Leu Pro Gly Val Ile Asp Ser His Thr His Phe Asn

50 55 60

Asp Pro Gly Phe Thr His Arg Glu Asp Phe Val Thr Gly Thr Ser Ser

65 70 75 80

Ala Ala Ala Gly Gly Val Thr Met Ile Val Asp Met Pro Cys Cys Ser

85 90 95

Val Pro Ser Val Arg Ser Ala Asp Asn Leu Phe Asn Lys Leu Asn Ala

100 105 110

Leu Glu Gly Lys Gly Ile Val Asp Tyr Ala Met Trp Gly Gly Ile Thr

115 120 125

Gly Glu Asp Val Arg Asn Asn Val Leu Pro Glu Ile Ile Ser Glu Gln

130 135 140

Ile Ala Asn Gly Val Val Ala Phe Lys Val Tyr Met Thr Pro Ser Val

145 150 155 160

Pro Ser Tyr Pro Arg Val Thr Asp Pro Glu Met Leu Glu Ala Phe Ala

165 170 175

Ala Val Ser Pro Thr Gly Leu Pro Ile Gly Ile His Ala Glu Asn Phe

180 185 190

Ala Ile Cys Asp Tyr Phe Thr Asn Lys Leu Lys Gly Glu Gly Arg Met

195 200 205

Asp Tyr Pro Ala Trp Ala Glu Ala Arg Met Ala Leu Ala Glu Lys Ala

210 215 220

Ala Ile Gln Leu Gly Ile Ser Phe Ala Glu Glu Thr Gly Ala Arg Leu

225 230 235 240

His Ile Val His Met Ser Thr Gly Ala Gly Asn Glu Leu Ile Arg Glu

245 250 255

Ala Lys Leu Lys Gly Thr Lys Val Thr Ala Glu Ser Cys Pro His Tyr

260 265 270

Leu Thr Leu Asn Ala Val Asp Ala Met Thr Glu Phe Gly Thr Phe Ala

275 280 285

Lys Ile Ala Pro Pro Leu Arg Thr Ala Lys Asp Asn Glu Val Leu Trp

290 295 300

Gln Gly Leu Thr Asp Gly Thr Ile Asp Phe Met Ala Thr Asp His Ala

305 310 315 320

Pro Tyr Glu Ile Ala Thr Glu Lys Asp Ala Pro Gly Met Asp Val Trp

325 330 335

Thr Ser Phe Pro Gly Ile Pro Gly Val Glu Thr Leu Val Pro Ile Met

340 345 350

Ile Ser Glu Gly Tyr Asn Lys Gly Arg Leu Ser Leu Ser Arg Leu Val

355 360 365

Glu Leu Leu Cys Thr Asn Pro Ala Ile His Tyr Gly Leu Tyr Pro Lys

370 375 380

Lys Gly Ala Leu Glu Ile Gly Thr Asp Ala Asp Phe Thr Ile Val Asp

385 390 395 400

Leu Glu Lys Glu Trp Thr Leu Asp Lys Asp Gln Met His Cys Lys Pro

405 410 415

Lys Tyr Thr Pro Phe His Gly Met Lys Leu Lys Gly Lys Val Asp Lys

420 425 430

Thr Ile Val Arg Gly Thr Leu Val Tyr Asp Asp Ala Leu Gly Val Val

435 440 445

Gly Lys Pro Gly His Gly Glu Tyr Val Lys Arg Gln Thr Ile Ser Glu

450 455 460

Leu Pro Arg Leu Leu Lys Tyr

465 470

<210> 29

<211> 486

<212> PRT

<213> 芽孢杆菌(Bacillus sp. )

<400> 29

Met Glu Leu Ile Leu Lys Asn Ala Asn Ile Pro Gln Gly Asp Arg Gln

1 5 10 15

Val Leu Thr Asn Ile Leu Ile Asn Asp Gly Lys Ile Val Gly Phe Ser

20 25 30

Asn Asp Ile Ser Phe Leu Asn Ala Glu Lys Val Ile Asp Ile Gly Gly

35 40 45

Lys Leu Val Val Pro Gly Cys Ile Asp Pro His Thr His Phe Met Asp

50 55 60

Pro Gly Phe Thr His Arg Glu Thr Phe Ala Thr Gly Ser Arg Ser Ala

65 70 75 80

Ile Ala Gly Gly Leu Thr Thr Ile Ile Asp Met Pro Cys Cys Ser Lys

85 90 95

Pro Ser Val Arg Asp Gly Glu Ser Phe Gln Lys Lys Ile Gly Pro Ile

100 105 110

Arg Asp Gln Ala Tyr Val Asp Tyr Cys Phe Trp Gly Gly Met Thr Gly

115 120 125

Glu Asp Ala Arg Glu Gly Trp Leu Asp Asn Val Tyr Pro Gln Ile Asp

130 135 140

Glu Gly Val Val Ala Phe Lys Val Tyr Met Thr Pro Ser Val Pro Thr

145 150 155 160

Phe Pro Arg Val Ser Asp Ala Glu Met Leu Glu Ile Phe Lys Ala Val

165 170 175

Ser Lys Thr Gly Leu Pro Ile Gly Val His Ala Glu Asn Tyr Asp Ile

180 185 190

Cys Thr Phe Asn Ser Lys Lys Leu Glu Lys Glu Gly Arg Phe Asp Gly

195 200 205

Pro Ala Trp Ala Glu Ala Arg Ser Thr Leu Ala Glu Lys Val Ala Ile

210 215 220

Glu Leu Ile Ile Ser Phe Ala Glu Ala Thr Asp Ala Arg Val His Val

225 230 235 240

Val His Met Thr Thr Lys Gly Gly Ala Glu Leu Val Lys Ala Ala Lys

245 250 255

Lys Arg Gly Val Lys Val Thr Ala Glu Thr Cys Pro His Tyr Leu Val

260 265 270

Leu Asn Ala Lys Asp Ser Met Ser Glu Arg Gly Ser Phe Ala Lys Ile

275 280 285

Ala Pro Pro Leu Arg Gly Lys Glu Asp Asn Glu Ala His Trp Gln Ala

290 295 300

Leu Ala Asp Gly Thr Ile Asp Phe Val Gly Thr Asp His Ala Pro Tyr

305 310 315 320

Glu Ile Ala Thr Glu Lys Asp Phe Pro Gly Ala Thr Ile Trp Asn Thr

325 330 335

Phe Pro Gly Ile Pro Gly Val Glu Thr Met Val Pro Ile Leu Val Ser

340 345 350

Glu Gly Leu Asn Lys Gly Arg Leu Ser Leu Ser Arg Phe Val Glu Val

355 360 365

Thr Ser Arg Asn Ala Ala Ile His Tyr Gly Ile Tyr Pro Lys Lys Gly

370 375 380

Ser Met Glu Ile Gly Ser Asp Ala Asp Phe Thr Val Ile Asp Leu Glu

385 390 395 400

Lys Glu Tyr Val Ile Asp Glu Gln Lys Thr Glu Ser Met Ala Lys Tyr

405 410 415

Asn Pro Leu His Gly Met Lys Leu Lys Gly Lys Pro Val Gln Thr Ile

420 425 430

Ile Arg Gly Asn Val Val Tyr Glu Asp Gly Lys Gly Val Leu Gly Gln

435 440 445

Ala Gly Tyr Gly Gln Tyr Val Pro Arg Gln Ser Ile Gln Arg Leu Glu

450 455 460

Arg Thr Phe Lys Tyr Glu Lys Phe Glu Ala Val Lys Val Glu Ala Lys

465 470 475 480

Glu Thr Val Lys Gln Asn

485

<210> 30

<211> 532

<212> PRT

<213> 极尖组织菌(Tissierella praeacuta)

<400> 31

Met Tyr Asp Val Ile Val Lys Asn Ala Arg Ile Pro Gln Gly Asp Asp

1 5 10 15

Thr Ala Leu Thr Asn Ile Leu Val Lys Asp Glu Lys Ile Ala Gly Phe

20 25 30

Val Asp Asn Ile Glu Gly Ile Glu Ala Lys Glu Ile Ile Asp Ala Lys

35 40 45

Gly His Leu Thr Leu Pro Gly Cys Ile Asp Ser His Thr His Phe Met

50 55 60

Tyr Gln Gly Phe Pro His Arg Glu Asn Phe Leu Thr Gly Thr Ala Ala

65 70 75 80

Ala Ala Arg Gly Gly Val Thr Thr Val Ile Asp Met Pro Cys Cys Ser

85 90 95

Val Pro Ser Ala Arg Ser Lys Glu Gln Leu Glu Leu Lys Leu Asp Leu

100 105 110

Ile Gln Pro Gln Ala Leu Val Asp Phe Ala Leu Trp Gly Gly Val Thr

115 120 125

Gly Glu Asp Val Arg Glu Gly Trp Leu Asp His Val Gln Glu Gln Ala

130 135 140

Asp Trp Gly Val Val Ala Phe Lys Ala Tyr Met Thr Pro Ser Val Pro

145 150 155 160

Thr Phe Pro Arg Val Thr Asp Pro Glu Met Phe Glu Ala Phe Lys Ala

165 170 175

Val Ala Lys Thr Gly Leu Pro Val Gly Ile His Ala Glu Asn Tyr Ser

180 185 190

Met Cys Asp Tyr Tyr Val Lys Glu Phe Gln Lys Gln Gly Arg Met Asp

195 200 205

Gly Pro Ala Trp Ala Glu Ala Arg Met Glu Leu Ala Glu Lys Val Ala

210 215 220

Ile Glu Leu Gly Ile Ser Phe Ala Glu Glu Ala Gly Ala Arg Leu His

225 230 235 240

Ile Val His Met Ser Thr Gly Ile Gly Ala Lys Leu Val Gly Glu Ala

245 250 255

Lys Lys Arg Gly Leu Asn Val Thr Ser Glu Thr Cys Pro His Tyr Leu

260 265 270

Thr Ile Asn Ala Gln Glu Ala Met Thr Lys Tyr Gly Ala Cys Ala Lys

275 280 285

Ile Ala Pro Pro Leu Arg Thr Lys Lys Asp Asn Glu Leu Leu Trp Glu

290 295 300

Gly Leu Asn Asn Gly Ser Val Asp Phe Ile Ala Thr Asp His Ala Pro

305 310 315 320

Tyr Glu Leu Glu Ser Glu Lys Ile Lys Glu Gly Thr Asn Ile Trp Thr

325 330 335

Ala Phe Pro Gly Ile Pro Gly Val Glu Thr Met Val Pro Val Leu Val

340 345 350

Ser Glu Gly Tyr Asn Lys Gly Arg Ile Ser Leu Ser Thr Leu Val Asp

355 360 365

Val Leu Ser Lys Asn Ala Ala Val His Tyr Gly Leu Tyr Pro Lys Lys

370 375 380

Gly Ala Met Asn Ile Gly Ser Asp Ala Asp Phe Thr Ile Ile Asp Leu

385 390 395 400

Asp Lys Glu Trp Val Ile Asp Pro Lys Asn Glu Ala Ser Met Cys Gly

405 410 415

Tyr Thr Pro Leu Glu Gly Ile Lys Leu Lys Gly Lys Val Ala Lys Thr

420 425 430

Ile Val Arg Gly His Leu Val Phe Glu Asp Leu Asp Glu Ser Thr Leu

435 440 445

Ala Lys Leu Thr Asp Glu Glu Leu Lys Lys Ile Val His Asn Phe Pro

450 455 460

Glu Gly Ile Glu Glu Lys Tyr Ala Glu Gln Phe Lys Lys Tyr Pro His

465 470 475 480

Leu Tyr Ser Ala Glu Tyr Glu Arg Ser Tyr Arg Ile Asn His Pro Glu

485 490 495

Val Ile Asp Glu His Ile Arg Gly Ile Lys Gly Ile Val Ala Lys Pro

500 505 510

Gly Phe Gly Lys Phe Val Lys Arg Gln Ser Ile Gln Ile Leu Pro Arg

515 520 525

Lys Ile Lys Tyr

530

<210> 31

<211> 486

<212> PRT

<213> 海泥芽孢杆菌(Bacillus oceanisediminis)

<400> 31

Met Asp Leu Ile Leu Lys Asn Ala Ser Ile Pro Gln Gly Asp Arg Gln

1 5 10 15

Val Leu Thr Asn Ile Leu Val Asn Glu Gly Lys Ile Val Gly Tyr Thr

20 25 30

Asn Asp Ile Ser Phe Leu Asn Ala Ala Arg Val Ile Asp Ile Lys Arg

35 40 45

Lys Leu Val Val Pro Gly Cys Ile Asp Pro His Thr His Phe Met Asp

50 55 60

Pro Gly Phe Thr His Arg Glu Thr Phe Ala Thr Gly Ser Arg Ser Ala

65 70 75 80

Ala Ala Gly Gly Leu Thr Thr Ile Ile Asp Met Pro Cys Cys Ser Lys

85 90 95

Pro Ser Val Arg Asp Gly Glu Ser Phe Asn Lys Lys Ile Gly Pro Ile

100 105 110

Arg Asp Gln Ala Tyr Ile Asp Tyr Cys Phe Trp Gly Gly Met Thr Gly

115 120 125

Glu Asp Ala Arg Glu Gly Trp Ile Asp Asn Ile Tyr Pro Gln Ile Asp

130 135 140

Gln Gly Val Val Ala Phe Lys Val Tyr Met Thr Pro Ser Val Pro Thr

145 150 155 160

Phe Pro Lys Val Ser Asp Ala Glu Met Leu Glu Ile Phe Arg Asn Val

165 170 175

Ala Lys Thr Gly Leu Pro Ile Gly Val His Ala Glu Asn Tyr Asp Ile

180 185 190

Cys Thr Phe Tyr Ser Lys Lys Leu Glu Lys Glu Gly Arg Leu Asp Gly

195 200 205

Pro Ala Trp Ala Glu Ala Arg Ser Ser Leu Ala Glu Lys Val Ala Ile

210 215 220

Glu Leu Ile Leu Ser Phe Ala Glu Ala Thr Asp Ala Arg Val His Val

225 230 235 240

Val His Met Ser Thr Lys Glu Gly Val Glu Leu Val Lys Ala Ala Lys

245 250 255

Lys Arg Gly Val Lys Val Thr Ala Glu Thr Cys Pro His Tyr Leu Val

260 265 270

Leu Asn Ala Lys Asp Ser Met Ser Glu Arg Gly Ser Tyr Ala Lys Ile

275 280 285

Ala Pro Pro Leu Arg Gly Lys Glu Asp Asn Glu Ser His Trp Gln Ala

290 295 300

Leu Ala Asp Gly Thr Ile Asp Phe Val Gly Thr Asp His Ala Pro Tyr

305 310 315 320

Glu Ile Ala Thr Glu Lys Asp Tyr Pro Gly Ala Thr Ile Trp Asn Thr

325 330 335

Phe Pro Gly Ile Pro Gly Val Glu Thr Met Val Pro Ile Leu Val Ser

340 345 350

Glu Gly Leu Asn Lys Gly Arg Leu Ser Leu Ser Arg Phe Val Glu Val

355 360 365

Thr Ser Arg Asn Ala Ala Ile His Tyr Gly Ile Tyr Pro Lys Lys Gly

370 375 380

Ser Met Glu Ile Gly Ser Asp Ala Asp Phe Thr Val Ile Asp Leu Glu

385 390 395 400

Lys Glu Tyr Val Ile Asp Glu Gln Lys Thr Glu Ser Met Ala Lys Tyr

405 410 415

Asn Pro Leu His Gly Met Lys Leu Lys Gly Lys Pro Val Gln Thr Ile

420 425 430

Ile Arg Gly Thr Val Ile Phe Asp Glu Asp Lys Gly Ile Val Gly Glu

435 440 445

Ala Gly Tyr Gly Glu Tyr Val Pro Arg Gln Ser Ile Gln Arg Leu Glu

450 455 460

Arg Thr Phe Lys Tyr Glu Lys Tyr Glu Ala Val Lys Ala Lys Thr Lys

465 470 475 480

Asp Pro Val Ala Gln Gln

485

<210> 32

<211> 493

<212> PRT

<213> 芽孢杆菌(Bacillus sp. )

<400> 32

Met Asp Leu Ile Leu Lys Asn Ala Met Ile Pro Gln Gly Asp Arg Thr

1 5 10 15

Val Ile Thr Asn Ile Leu Val His Glu Gly Lys Ile Thr Gly Phe Cys

20 25 30

Ser Asp Ile His Phe Leu Lys Ala Asp Lys Val Ile Asp Met Glu Gly

35 40 45

Lys Leu Val Val Pro Gly Cys Ile Asp Pro His Thr His Phe Met Asp

50 55 60

Pro Gly Phe Thr His Arg Glu Thr Phe Ala Thr Gly Ser Arg Ser Ala

65 70 75 80

Ala Ala Gly Gly Leu Thr Thr Ile Ile Asp Met Pro Cys Cys Ser Lys

85 90 95

Pro Ser Val Arg Asp Gly Glu Ser Phe Gln Lys Lys Ile Ala Pro Ile

100 105 110

Arg Gly Gln Ala Tyr Ile Asp Tyr Cys Phe Trp Gly Gly Met Thr Gly

115 120 125

Glu Asp Ala Arg Asn Gly Trp Ile Asp Asn Ile Tyr Pro Gln Met Asp

130 135 140

Glu Gly Val Val Ala Phe Lys Val Tyr Met Thr Pro Ser Val Pro Thr

145 150 155 160

Phe Pro Lys Val Ser Asp Gly Glu Met Leu Glu Ile Phe Gln Lys Val

165 170 175

Ala Lys Thr Gly Met Pro Ile Gly Val His Ala Glu Asn Tyr Asp Ile

180 185 190

Cys Thr Phe Tyr Thr Glu Lys Leu Lys Lys Glu Gly Arg Glu Asp Gly

195 200 205

Pro Ala Trp Ala Glu Ala Arg Met Ala Leu Ala Glu Lys Val Ala Ile

210 215 220

Glu Leu Ile Ile Ser Tyr Ala Glu Ala Thr Asp Ala Arg Val His Val

225 230 235 240

Val His Met Ser Thr Lys Glu Gly Val Lys Leu Val Arg Glu Ala Lys

245 250 255

Lys Arg Gly Val Lys Val Thr Ala Glu Thr Cys Pro His Tyr Leu Met

260 265 270

Leu Asn Ala Gln Asp Ser Met Ser Glu Arg Gly Ala Phe Ala Lys Ile

275 280 285

Ala Pro Pro Leu Arg Gly Lys Glu Asp Asn Gln Ala His Trp Gln Ala

290 295 300

Leu Ala Asp Gly Thr Ile Asp Phe Val Gly Thr Asp His Ala Pro Tyr

305 310 315 320

Glu Ile Ala Thr Glu Lys Ala Ala Ala Asp Ser Thr Ile Trp Asn Ser

325 330 335

Phe Pro Gly Ile Pro Gly Val Glu Thr Met Val Pro Ile Leu Val Ser

340 345 350

Glu Gly Leu Asn Lys Gly Arg Leu Ser Leu Ser Arg Phe Val Glu Val

355 360 365

Thr Ser Lys Asn Ala Ala Ile His Tyr Gly Ile Tyr Pro Lys Lys Gly

370 375 380

Ala Met Glu Ile Gly Ser Asp Ala Asp Phe Thr Val Ile Asp Leu Asp

385 390 395 400

Cys Glu Trp Glu Ile Asp Glu Lys Gln Thr Glu Ser Met Ala Lys Tyr

405 410 415

Asn Pro Leu His Gly Ile Lys Leu Lys Gly Arg Pro Val Gln Thr Ile

420 425 430

Val Arg Gly Asn Met Val Tyr Asn Tyr Glu Ala Gly Gly Ile Val Gly

435 440 445

Glu Ala Gly Tyr Gly Glu Tyr Val Lys Arg Gln Ser Ile Gln Arg Leu

450 455 460

Asp Arg Thr Leu Lys Tyr Glu Asn Tyr Glu Gly Ala Ile Gln Thr Ala

465 470 475 480

Ala Pro Lys Ser Gly Arg Gln Gln Leu Glu Lys Ser Lys

485 490

<210> 33

<211> 471

<212> PRT

<213> Anaeromicrobium sediminis

<400> 33

Met Asn Tyr Asp Val Ile Val Lys Asn Ala Arg Ile Pro Gln Gly Asn

1 5 10 15

Asp Thr Val Leu Thr Asn Ile Leu Val Lys Asp Glu Lys Ile Val Gly

20 25 30

Phe Thr Leu Asp Leu Asp Gly Ile Gln Gly Asn Lys Val Ile Asp Ala

35 40 45

Glu Gly His Leu Thr Leu Pro Gly Cys Ile Asp Ser His Thr His Ile

50 55 60

Asn Asp Pro Gly Phe Thr His Arg Glu Asn Phe Leu Thr Gly Thr Ser

65 70 75 80

Ala Ala Ala Ser Gly Gly Val Thr Thr Ile Val Asp Met Pro Cys Cys

85 90 95

Ser Val Pro Ser Val Arg Ser Val Glu Asn Leu Glu His Lys Leu Asn

100 105 110

Ala Ile Lys Asn Lys Ala Ile Val Asp Tyr Gly Met Trp Gly Gly Val

115 120 125

Thr Gly Glu Asp Val Arg Asn Gly Trp Leu His Asn Val Lys Glu Gln

130 135 140

Ala Asp Tyr Gly Val Leu Ala Phe Lys Val Tyr Met Thr Pro Ser Val

145 150 155 160

Pro Thr Tyr Pro Arg Val Thr Asp Pro Glu Met Leu Glu Ala Phe Lys

165 170 175

Ala Val Ala Glu Thr Gly Leu Pro Ile Gly Ile His Ala Glu Asn Phe

180 185 190

Ala Met Cys Asp Phe Tyr Val Lys Lys Phe Glu Lys Glu Gly Arg Thr

195 200 205

Asp Gly Pro Ala Trp Ala Glu Ala Arg Met Thr Leu Ala Glu Lys Val

210 215 220

Ala Ile Glu Leu Gly Ile Ser Phe Ala Glu Glu Ala Gly Ala Arg Leu

225 230 235 240

His Ile Val His Met Ser Thr Gly Val Gly Ala Lys Leu Val Gly Asp

245 250 255

Ala Lys Lys Arg Gly Leu Asp Val Thr Ala Glu Thr Cys Pro His Tyr

260 265 270

Leu Thr Leu Asn Tyr Lys Asp Ala Met Thr Glu Phe Met Gln Phe Ala

275 280 285

Lys Ile Ala Pro Pro Leu Arg Thr Lys Ser Asp Asn Glu Glu His Trp

290 295 300

Lys Ala Leu Gln Asp Gly Arg Ile Asp Phe Val Ala Thr Asp His Ala

305 310 315 320

Pro Tyr Glu Ile Pro Thr Glu Lys Glu Ala Glu Gly Met His Ile Trp

325 330 335

Asn Ala Phe Pro Gly Ile Pro Gly Val Glu Thr Met Val Pro Met Ile

340 345 350

Val Ser Glu Gly Tyr Asn Lys Gly Arg Leu Ser Leu Ser Arg Leu Val

355 360 365

Glu Val Leu Cys Ser Ser Pro Ala Lys His Tyr Gly Ile Tyr Pro Lys

370 375 380

Lys Gly Ala Met Phe Ile Gly Ser Asp Ala Asp Phe Thr Ile Ile Asp

385 390 395 400

Leu Glu Lys Glu Trp Thr Ile Asp Lys Asp Lys Met Tyr Thr Met Ala

405 410 415

Lys Tyr Thr Pro Phe His Gly Tyr Lys Leu Lys Gly Lys Pro Val Lys

420 425 430

Thr Val Val Arg Gly Lys Leu Val Tyr Asp Asp Glu Ser Gly Ile Val

435 440 445

Gly Glu Glu Gly Tyr Gly Glu Phe Ile Arg Arg Gln Asn Ile Gln Lys

450 455 460

Leu Asp Lys Ala Ile Lys Tyr

465 470

<210> 34

<211> 476

<212> PRT

<213> 解脂嗜热互营杆菌(Thermosyntropha lipolytica))

<400> 34

Met Leu Asp Thr Ile Val Lys Asn Ala Met Ile Pro Gln Gly Asp Glu

1 5 10 15

Leu Val Lys Ala Asn Ile Leu Val Glu Asp Gly Lys Ile Val Gly Ile

20 25 30

Thr Arg Lys Glu Phe Glu Ala Arg Asn Val Ile Asp Ala Glu Gly Asn

35 40 45

Met Val Leu Pro Gly Cys Ile Asp Ser His Thr His Phe Met Asp Pro

50 55 60

Gly Phe Thr His Arg Glu Thr Phe Thr Thr Gly Cys Arg Ala Ala Ala

65 70 75 80

Tyr Gly Gly Ile Thr Thr Val Ile Asp Met Pro Cys Cys Ser Val Pro

85 90 95

Ser Val Arg Gly Val Pro Glu Met Glu His Lys Leu Glu Ala Ile Lys

100 105 110

Pro Gln Ala Leu Ile Asp Phe Ala Leu Trp Gly Gly Val Thr Gly Glu

115 120 125

Asp Val Arg Glu Glu Lys His Phe Arg Ile Val Arg Glu Gln Ala Glu

130 135 140

Ala Gly Val Val Ala Phe Lys Val Tyr Met Thr Pro Ser Val Pro Ser

145 150 155 160

Tyr Pro Arg Val Thr Asp Pro Glu Met Leu Glu Thr Phe Lys Ala Val

165 170 175

Ala Glu Thr Gly Leu Pro Val Gly Ile His Ala Glu Asn Phe Ala Met

180 185 190

Thr Asp Phe Tyr Thr Gln Lys Phe Ile Arg Glu Gly Arg Leu Asp Ala

195 200 205

Pro Ala Trp Ala Glu Ala Arg Ser Ile Leu Ala Glu Lys Val Ala Ile

210 215 220

Glu Leu Gly Ile Ser Phe Ala Glu Glu Val Gly Ala Arg Leu His Ile

225 230 235 240

Val His Met Ser Thr Gly Ile Gly Ala Arg Leu Val Gly Glu Ala Lys

245 250 255

Met Arg Gly Leu Asp Val Thr Ser Glu Thr Cys Pro His Tyr Leu Val

260 265 270

Leu Asn Tyr Lys Asp Ala Met Ser Glu Tyr Gly Ala Phe Ala Lys Ile

275 280 285

Ala Pro Pro Leu Arg Thr Glu Glu Asp Asn Glu Ile Leu Trp Glu Gly

290 295 300

Leu Gln Asn Gly Ser Val Asp Phe Ile Ala Thr Asp His Ala Pro Tyr

305 310 315 320

Glu Ile Ala Thr Glu Lys Ala Lys Gln Gly Met Thr Ile Trp Thr Ala

325 330 335

Phe Pro Gly Ile Pro Gly Val Glu Thr Met Val Pro Ile Leu Val Ser

340 345 350

Glu Gly Tyr Asn Lys Gly Arg Leu Thr Leu Ser Gln Leu Val Asp Val

355 360 365

Leu Ser Arg Asn Ala Ala Ile His Tyr Gly Leu Tyr Pro Lys Lys Gly

370 375 380

Ala Met Phe Ile Gly Ser Asp Ala Asp Phe Thr Val Ile Asp Leu Asp

385 390 395 400

Arg Glu Trp Ile Ile Asn Lys Glu Asp Gln Gln Ser Met Ala Lys Tyr

405 410 415

Thr Pro Leu His Gly Met Lys Leu Lys Gly Lys Val Val Lys Thr Ile

420 425 430

Val Arg Gly Lys Val Val Phe Asp Glu Lys Lys Gly Ile Met Val Asn

435 440 445

Pro Gly Tyr Gly Gln Phe Val Lys Arg Gln Thr Ile Ser Lys Leu Asp

450 455 460

Lys Val Ile Arg Phe Pro Ser Ile Asn Asp Ile Phe

465 470 475

<210> 35

<211> 471

<212> PRT

<213> Alkaliphilus peptidifermentans

<400> 35

Met Lys Tyr Asp Val Val Val Lys Asn Ala Lys Ile Pro Gln Gly Asp

1 5 10 15

Glu Thr Val Met Thr His Ile Leu Val Lys Asp Glu Lys Ile Ala Gly

20 25 30

Phe Val Thr Asn Leu Asp Gly Ile Glu Tyr Asn Ser Glu Ile Asp Ala

35 40 45

Glu Gly Asn Leu Thr Leu Pro Gly Cys Phe Asp Ser His Thr His Phe

50 55 60

Met Asp Pro Gly Phe Thr His Arg Glu Thr Phe Leu Thr Gly Ser Ser

65 70 75 80

Ser Ala Ala Ala Gly Gly Leu Thr Thr Ile Met Asp Met Pro Cys Cys

85 90 95

Ser Lys Pro Ser Val Arg Gly Val Glu Glu Leu Glu Ser Lys Leu Asn

100 105 110

Ala Ile Lys Asp Gln Ala Val Ile Asp Tyr Ala Met Trp Gly Gly Val

115 120 125

Thr Gly Glu Asp Ile Arg Ala Gly Lys Lys His Ile Ile Lys Glu Gln

130 135 140

Ala Asp Tyr Gly Val Cys Ala Phe Lys Val Tyr Met Thr Pro Ser Val

145 150 155 160

Pro Thr Tyr Glu Arg Val Asn Asp Pro Glu Met Leu Glu Ala Phe Arg

165 170 175

Trp Val Ala Glu Thr Gly Val Pro Val Gly Ile His Ala Glu Asn Phe

180 185 190

Ala Met Cys Asp Tyr Phe Val Lys Lys Phe Gln Lys Glu Gly Arg Thr

195 200 205

Asp Gly Pro Ala Trp Ala Glu Ala Arg Leu Val Glu Ala Glu Gln Ser

210 215 220

Ala Ile Glu Leu Gly Ile Asn Phe Ala Lys Ser Ser Gly Ala Arg Leu

225 230 235 240

His Ile Val His Met Ser Ser Gly Ile Gly Ala Lys Leu Val Gly Glu

245 250 255

Ala Lys Lys Lys Gly Leu Asp Val Thr Ser Glu Thr Cys Pro His Tyr

260 265 270

Leu Met Leu Asn Tyr Gln Asp Ala Met Thr Glu His Lys Gln Phe Ala

275 280 285

Lys Ile Ala Pro Pro Leu Arg Thr Lys Lys Asp Asn Glu Glu Leu Trp

290 295 300

Glu Gly Leu Gln Asn Gly Ser Val Asp Phe Met Ala Thr Asp His Ala

305 310 315 320

Pro Tyr Glu Ile Glu Thr Glu Lys Asp Ala Pro Gly Met Asp Ile Trp

325 330 335

Thr Ala Phe Pro Gly Ile Pro Gly Val Glu Thr Leu Val Pro Ile Leu

340 345 350

Val Ser Glu Gly Tyr Asn Lys Gly Arg Leu Thr Leu Gly Arg Leu Val

355 360 365

Glu Val Leu Ser Ser Ser Pro Ala Lys Met Tyr Gly Leu Tyr Pro Lys

370 375 380

Lys Gly Ala Leu Met Ile Gly Ser Asp Ala Asp Phe Thr Ile Ile Asp

385 390 395 400

Leu Glu Arg Glu Trp Thr Ile Asp Gln Lys Lys Thr Glu Ser Met Ala

405 410 415

Lys Tyr Asn Pro Leu His Gly Ile Lys Leu Lys Gly Lys Pro Val Lys

420 425 430

Thr Val Val Arg Gly Thr Val Val Tyr Asp Asp Lys Glu Gly Val Ile

435 440 445

Gly Lys Pro Gly Phe Gly Gln Phe Val Lys Arg Gln Arg Ile Gln Lys

450 455 460

Leu Glu Arg Thr Ile Lys Tyr

465 470

<210> 36

<211> 490

<212> PRT

<213> 米氏解硫胺素芽孢杆菌(Aneurinibacillus migulanus)

<400> 36

Met Asp Leu Ile Leu Lys Asn Ala Asn Ile Pro Gln Gly Asp Arg Gln

1 5 10 15

Val Gln Thr Asn Ile Leu Val Glu Asn Gly Thr Ile Val Gly Phe Val

20 25 30

Asn Ser Ile Asn Gly Leu Gln Ala Lys Asn Val Ile Asp Leu Lys Gly

35 40 45

Lys Leu Val Val Pro Gly Cys Ile Asp Pro His Thr His Phe Met Asp

50 55 60

Pro Gly Phe Thr His Arg Glu Thr Phe Ala Thr Gly Thr Met Ser Ala

65 70 75 80

Ala Ala Gly Gly Leu Thr Thr Ile Ile Asp Met Pro Cys Cys Ser Lys

85 90 95

Pro Ser Val Arg Ser Glu Glu Ser Phe Tyr Ala Lys Ile Asp Pro Ile

100 105 110

Lys Asp Lys Ala Tyr Ile Asp Tyr Cys Phe Trp Gly Gly Met Thr Gly

115 120 125

Glu Asp Val Arg Glu Gly Leu Leu Asp Asn Val Trp Gly Gln Lys Glu

130 135 140

Ala Gly Val Val Ala Phe Lys Ser Tyr Met Thr Pro Ser Val Pro Thr

145 150 155 160

Phe Pro Lys Val Ser Asp Ala Glu Leu Leu Glu Ile Phe Tyr Lys Val

165 170 175

Ala Glu Thr Gly Leu Pro Val Gly Val His Ala Glu Asn Tyr Asp Ile

180 185 190

Cys Ser Phe Tyr Thr Glu Lys Leu Lys Lys Glu Gly Arg Leu Asp Gly

195 200 205

Pro Ala Trp Ala Glu Ala Arg Lys Ala Leu Ala Glu Lys Val Ala Ile

210 215 220

Glu Leu Ile Ile Ser Tyr Ala Glu Glu Thr Gly Ala Arg Ala His Ile

225 230 235 240

Val His Met Ser Thr Lys Glu Gly Ala His Leu Ile Arg Gln Ala Lys

245 250 255

Glu Arg Gly Val Lys Ile Thr Ala Glu Thr Cys Pro His Tyr Leu Val

260 265 270

Leu Asn Ala Gln Glu Ser Met Thr Lys Tyr Gly Thr Phe Ala Lys Ile

275 280 285

Ala Pro Pro Leu Arg Gly Val Glu Asp Asn Glu Ile His Trp Gln Ala

290 295 300

Leu Ala Asp Gly Thr Ile Asp Phe Val Gly Thr Asp His Ala Pro Tyr

305 310 315 320

Glu Ile Pro Thr Glu Lys Ala Ala Glu Asp Ser Asp Ile Trp Asn Ser

325 330 335

Phe Pro Gly Ile Pro Gly Val Glu Thr Met Val Pro Ile Leu Val Ser

340 345 350

Glu Gly Leu Asn Lys Gly Arg Leu Ser Leu Ser Arg Phe Val Glu Val

355 360 365

Thr Ser Arg Asn Ala Ala Ile His Tyr Gly Ile Tyr Pro Lys Lys Gly

370 375 380

Ala Ile Glu Ile Gly Ser Asp Ala Asp Phe Thr Val Ile Asp Leu Glu

385 390 395 400

Lys Glu His Val Ile Asp Gln Glu Gln Thr Tyr Ser Met Ala Lys Tyr

405 410 415

Asn Pro Leu His Asp Ile Lys Leu Lys Gly Met Pro Val Met Thr Ile

420 425 430

Val Arg Gly Asn Val Val Tyr Glu Asp Gly Lys Gly Ile Val Gly Gln

435 440 445

Glu Gly Tyr Gly Gln Tyr Val Lys Arg Gln Ser Thr Gln Gln Leu Asp

450 455 460

Ala Val Ile Asn Leu Thr Lys Ser Asn Glu Ser Lys Ala Val Ser Gln

465 470 475 480

Ser Ser Glu Lys Lys Glu Ser Val Lys Leu

485 490

<210> 37

<211> 470

<212> PRT

<213> 类芽孢杆菌(Paenibacillus sp.)

<400> 37

Met Gly Tyr Glu Leu Ile Ile Lys Asn Ala Ser Ile Pro Gln Gly Asp

1 5 10 15

Arg Gln Val Leu Thr Asp Ile Leu Val Asn Lys Gly Val Ile Val Gly

20 25 30

Phe Ala Lys Asp Ile Asp Ala Glu Ala Asp Thr Ile Ile Asp Ala Gln

35 40 45

Gly Lys Leu Val Leu Pro Gly Val Ile Asp Ser His Thr His Phe Asn

50 55 60

Asp Pro Gly Phe Thr His Arg Glu Asp Phe Val Thr Gly Thr Ser Ser

65 70 75 80

Ala Ala Ala Gly Gly Val Thr Met Ile Val Asp Met Pro Cys Cys Ser

85 90 95

Val Pro Ser Val Arg Ser Val Glu Asn Leu His Asn Lys Leu Asn Ala

100 105 110

Leu Glu Gly Lys Gly Ile Val Asp Tyr Ala Met Trp Gly Gly Ile Thr

115 120 125

Gly Glu Asp Val Arg Asn Asn Val Leu Asp Ile Val Lys Glu Gln Ala

130 135 140

Ala Glu Gly Val Val Ala Phe Lys Val Tyr Met Thr Pro Ser Val Pro

145 150 155 160

Ser Tyr Pro Arg Val Thr Asp Pro Glu Met Leu Glu Ala Phe Tyr Ala

165 170 175

Val Ala Glu Thr Gly Met Pro Ile Gly Ile His Ala Glu Asn Tyr Ala

180 185 190

Ile Cys Asp Tyr Tyr Thr Gln Lys Leu Lys Lys Glu Gly Arg Met Asp

195 200 205

Tyr Pro Ala Trp Ala Glu Ala Arg Lys Ile Leu Ala Glu Lys Ala Ala

210 215 220

Ile Gln Leu Gly Ile Ser Phe Ser Glu Glu Ser Gly Ala Arg Leu His

225 230 235 240

Ile Val His Met Ser Thr Ala Ala Gly Asn Gln Leu Ile Lys Glu Ala

245 250 255

Lys Leu Lys Gly Val Lys Val Thr Ala Glu Ser Cys Pro His Tyr Leu

260 265 270

Thr Leu Asn Ala Val Asp Ala Met Thr Glu Phe Gly Thr Phe Ala Lys

275 280 285

Ile Ala Pro Pro Leu Arg Arg Pro Glu Asp Asn Glu Ala Leu Trp Gln

290 295 300

Gly Leu Val Asp Gly Thr Ile Asp Phe Met Ala Thr Asp His Ala Pro

305 310 315 320

Tyr Glu Ile Ala Thr Glu Lys Asp Ala Pro Gly Met Asp Val Trp Thr

325 330 335

Ser Phe Pro Gly Ile Pro Gly Val Glu Thr Leu Val Pro Ile Met Ile

340 345 350

Ser Glu Gly Tyr Asn Lys Gly Arg Leu Ser Leu Ser Arg Leu Val Glu

355 360 365

Leu Leu Cys Thr Asn Pro Ala Ile His Tyr Gly Leu Tyr Pro Lys Lys

370 375 380

Gly Ala Leu Glu Ile Gly Thr Asp Ala Asp Phe Thr Ile Val Asp Leu

385 390 395 400

Glu Lys Glu Trp Thr Phe Asp Lys Asp Lys Met His Cys Lys Pro Lys

405 410 415

Tyr Thr Pro Phe His Gly Met Lys Leu Lys Gly Lys Val Asp Lys Thr

420 425 430

Ile Val Arg Gly Thr Leu Val Tyr Asp Asp Glu Leu Gly Val Val Gly

435 440 445

Lys Pro Gly Tyr Gly Glu Phe Val Lys Arg Gln Thr Ile Ser Glu Leu

450 455 460

Pro Arg Leu Leu Lys Tyr

465 470

<210> 38

<211> 516

<212> PRT

<213> Marinisporobacter balticus

<400> 38

Met Lys Val Asp Val Ile Val Lys Asn Ala Arg Ile Pro Gln Gly Asp

1 5 10 15

Asp Thr Ile Leu Thr Asn Ile Leu Val Lys Asp Glu Lys Ile Ala Gly

20 25 30

Phe Thr Gln Asp Leu Thr Asp Val Glu Ala Gly Asn Ile Ile Asp Ala

35 40 45

Gln Gly Asn Leu Thr Met Pro Gly Cys Ile Asp Ser His Thr His Phe

50 55 60

Met Tyr Gln Gly Phe Gly His Arg Glu Asn Thr Leu Thr Gly Ser Ala

65 70 75 80

Ala Ala Ala Thr Gly Gly Val Thr Thr Val Ile Asp Met Pro Cys Cys

85 90 95

Ser Val Pro Ser Val Arg Ser Val Glu Gln Leu Asn Thr Lys Val Ser

100 105 110

Leu Trp Lys Pro Gln Ala Leu Val Asp Phe Ala Leu Trp Gly Gly Val

115 120 125

Thr Gly Glu Asp Val Arg Glu Gly Trp Leu His Asn Val Lys Glu Gln

130 135 140

Val Asp Ala Gly Val Val Ala Phe Lys Leu Tyr Met Thr Pro Ser Val

145 150 155 160

Pro Thr Tyr Pro Arg Val Ser Asp Pro Glu Met Phe Glu Cys Phe Lys

165 170 175

Ala Val Ala Glu Thr Gly Leu Pro Val Gly Ile His Ala Glu Asn Phe

180 185 190

Ala Met Cys Asp Phe Tyr Val Lys Lys Tyr Gln Glu Glu Gly Arg Leu

195 200 205

Asp Gly Pro Ala Trp Ala Glu Ala Arg Ser Ile Leu Ala Glu Lys Thr

210 215 220

Ala Ile Gln Leu Gly Ile Ser Phe Ala Glu Glu Ser Gly Ala Arg Leu

225 230 235 240

His Ile Val His Met Ser Thr Gly Ile Gly Ala Gln Leu Val Gly Glu

245 250 255

Ala Lys Lys Arg Gly Leu Ser Val Thr Ala Glu Thr Cys Pro His Tyr

260 265 270

Leu Thr Leu Asn Ala Gln Glu Ala Met Thr Glu His Gly Ala Cys Ala

275 280 285

Lys Ile Ala Pro Pro Leu Arg Arg Lys Glu Asp Asn Glu Met His Trp

290 295 300

Glu Ala Leu Arg Asn Gly Ser Ile Asp Phe Val Ala Thr Asp His Ala

305 310 315 320

Pro Tyr Glu Ile Glu Ser Glu Lys Ala Lys Ala Gly Thr Asn Ile Trp

325 330 335

Thr Ala Phe Pro Gly Ile Pro Gly Val Glu Thr Met Val Pro Val Leu

340 345 350

Val Ser Glu Gly Tyr Asn Lys Gly Arg Ile Ser Leu Ser Arg Leu Val

355 360 365

Glu Val Leu Ser Lys Asn Ala Ala Ile His Tyr Gly Leu Tyr Pro Lys

370 375 380

Lys Gly Ala Met Phe Ile Gly Ser Asp Ala Asp Phe Thr Ile Ile Asp

385 390 395 400

Leu Asp Arg Ala Trp Thr Ile Lys Gln Glu Glu Gln Val Thr Leu Ala

405 410 415

Lys Tyr Asn Pro Leu His Gly Met Lys Leu Lys Gly Lys Pro Val Lys

420 425 430

Thr Ile Val Arg Gly Asn Leu Val Tyr Ser Asp Gly Glu Glu Gly Val

435 440 445

Leu Pro Ser Tyr Asp Asn Trp Asp Val Val Phe Asn Thr Pro Asp Asp

450 455 460

Leu Gln Ser Ala Glu Tyr Gln Val Ser Tyr Arg Lys Asp His Pro Glu

465 470 475 480

Lys Val Lys Lys Glu Val Gln Ser Ile Lys Gly Ile Met Val Glu Pro

485 490 495

Gly Phe Gly Thr Trp Ile Lys Arg Gln Ser Ile Gln Lys Leu Asp Lys

500 505 510

Thr Ile Lys Phe

515

<210> 39

<211> 497

<212> PRT

<213> 土地芽孢杆菌(Bacillus terrae)

<400> 39

Met Asp Leu Ile Leu Lys Asn Ala Leu Ile Pro Gln Gly Asp Arg Lys

1 5 10 15

Val Lys Thr Asn Ile Leu Val Asp Asn Gly Lys Ile Val Gly Phe Ile

20 25 30

Ser Ala Leu Asn Gly Leu Ser Ala Asp Arg Val Ile Asp Met Lys Glu

35 40 45

Asn Leu Val Val Pro Gly Cys Ile Asp Pro His Thr His Phe Met Asp

50 55 60

Pro Gly Phe Thr His Arg Glu Thr Phe Thr Thr Gly Thr Arg Ser Ala

65 70 75 80

Ala Ala Gly Gly Val Thr Thr His Ile Asp Met Pro Cys Cys Ser Lys

85 90 95

Pro Ser Val Arg Asp Ala Glu Ser Phe His Lys Lys Leu Ala Pro Leu

100 105 110

Lys Asp Gln Ala Tyr Ile Asp Phe Ala Phe Trp Gly Gly Met Thr Gly

115 120 125

Glu Asp Val Arg Glu Gly Leu Leu Asp Asn Ile Gln Pro Gln Ile Asp

130 135 140

Glu Gly Val Val Ala Phe Lys Val Tyr Met Thr Pro Ser Val Pro Thr

145 150 155 160

Phe Pro Lys Val Ser Asp Ala Glu Met Leu Glu Ile Phe Ser His Ile

165 170 175

Ala Pro Thr Gly Met Pro Ile Gly Val His Ala Glu Asn Tyr Asp Ile

180 185 190

Cys Thr Phe Tyr Ser Glu Lys Leu Lys Lys Glu Gly Arg Leu Asp Gly

195 200 205

Pro Ala Trp Ala Glu Ala Arg Met Ala Leu Ala Glu Lys Ala Ala Ile

210 215 220

Gln Leu Ile Ile Ser Phe Ala Glu Ala Thr Gly Ala Arg Val His Val

225 230 235 240

Val His Met Ser Thr Lys Glu Gly Val Glu Leu Val Arg Glu Ala Lys

245 250 255

Lys Arg Gly Val Lys Val Thr Ala Glu Thr Cys Pro His Tyr Leu Met

260 265 270

Leu Asn Ala Glu Asp Ser Met Ser Glu Arg Gly Thr Phe Ala Lys Ile

275 280 285

Ala Pro Pro Leu Arg Glu Lys Glu Asp Asn Ile Val His Trp Gln Ala

290 295 300

Leu Ala Asp Gly Thr Ile Asp Phe Val Gly Thr Asp His Ala Pro Tyr

305 310 315 320

Glu Ile Pro Thr Glu Lys Asp Ala Gln Asp Ser Thr Ile Trp Asn Ser

325 330 335

Phe Pro Gly Ile Pro Gly Val Glu Thr Met Val Pro Ile Leu Val Ser

340 345 350

Glu Gly Leu Asn Lys Gly Arg Leu Ser Leu Ser Arg Phe Val Glu Val

355 360 365

Thr Ser Arg Asn Ala Ala Ile His Phe Gly Ile Tyr Pro Lys Lys Gly

370 375 380

Ala Met Glu Ile Gly Ser Asp Ala Asp Phe Thr Val Ile Asp Leu Glu

385 390 395 400

Arg Glu Tyr Thr Ile Asp Glu Gln Lys Thr Glu Ser Met Ala Lys Tyr

405 410 415

Asn Pro Leu His Gly Leu Lys Leu Lys Gly Lys Pro Ile Gln Thr Ile

420 425 430

Val Arg Gly Gln Val Val Tyr Asp Glu Asp Asn Gly Gly Ile Thr Gly

435 440 445

Ala Ala Gly Tyr Gly Gln Phe Val Asn Arg Gln Ser Ile Gln Ser Leu

450 455 460

Glu Arg Val Ile Lys Tyr Glu Thr Tyr Glu Glu Arg Ala Lys Glu Ala

465 470 475 480

Glu Ala Ser Pro Arg Ile Glu Lys Ala Leu Arg Arg Asp Leu Ile Thr

485 490 495

Asn

<210> 40

<211> 482

<212> PRT

<213> 加利福尼亚丁达尔氏菌(Tindallia californiensis)

<400> 40

Met Lys Val Asp Val Val Val Thr Asn Ala Arg Ile Pro Gln Gly Asp

1 5 10 15

Asp Thr Val Leu Thr Asn Ile Leu Val Lys Asp Glu Lys Ile Ala Gly

20 25 30

Phe Val Ser Glu Leu Asp Gly Val Thr Tyr Asp Arg Glu Ile Asp Ala

35 40 45

Lys Gly His Leu Thr Leu Pro Gly Cys Phe Asp Ser His Thr His Phe

50 55 60

Met Asp Pro Gly Phe Glu His Arg Glu Asn Phe Leu Thr Gly Thr Ser

65 70 75 80

Ser Ala Ala Ala Gly Gly Leu Thr Thr Ile Met Asp Met Pro Cys Cys

85 90 95

Ser Lys Pro Ser Val Arg Ser Ile Pro Glu Leu Glu Ser Lys Leu Asn

100 105 110

Arg Ile Lys Asp Lys Ala Ile Ile Asp Phe Ala Phe Trp Gly Gly Val

115 120 125

Thr Gly Glu Asp Ile Arg Asn Gly Asp Tyr His Val Val Gln Glu Gln

130 135 140

Ala Asp Tyr Gly Val Cys Ala Phe Lys Val Tyr Met Thr Pro Ser Val

145 150 155 160

Pro Thr Tyr Glu Arg Val Thr Asp Pro Glu Met Leu Glu Ala Phe Arg

165 170 175

Leu Val Ala Lys Thr Gly Leu Val Val Gly Ile His Ala Glu Asn Phe

180 185 190

Ala Met Cys Asp Phe Tyr Val Lys Lys Phe Gln Ala Glu Gly Arg Thr

195 200 205

Asp Gly Pro Ala Trp Ala Glu Ala Arg Leu Glu Leu Ala Glu Lys Ala

210 215 220

Ala Ile Gln Leu Gly Ile Ser Phe Ala Glu Glu Thr Gly Ala Arg Leu

225 230 235 240

His Ile Val His Met Ser Thr Gly Ile Gly Ala Lys Leu Val Gly Ala

245 250 255

Ala Lys Gln Lys Gly Leu Asp Val Thr Ser Glu Ala Cys Pro His Tyr

260 265 270

Leu Met Leu Asn Tyr Gln Asp Ser Met Thr Glu His Arg Gln Phe Ala

275 280 285

Lys Ile Ala Pro Pro Leu Arg Thr Lys Lys Asp Asn Glu Glu Leu Trp

290 295 300

Glu Gly Leu Gln Asn Gly Ser Val Asp Phe Met Gly Thr Asp His Ala

305 310 315 320

Pro Tyr Glu Ile Glu Thr Glu Lys Glu Gly Ile Val Asp Gly Val Ala

325 330 335

Leu Asp Ile Trp Asn Ser Phe Pro Gly Ile Pro Gly Val Glu Thr Leu

340 345 350

Val Pro Ile Met Val Ser Glu Gly Tyr Leu Lys Gly Arg Leu Ser Leu

355 360 365

Ser Arg Leu Val Glu Val Leu Ser Thr Asn Ala Ala Lys Gln Tyr Gly

370 375 380

Ile Tyr Pro Lys Lys Gly Ala Leu His Ile Gly Ser Asp Ala Asp Phe

385 390 395 400

Thr Ile Ile Asp Leu Glu Lys Glu Trp Thr Ile Asp Gln Lys Glu Thr

405 410 415

Tyr Ser Met Ala Lys Tyr Asn Pro Leu His Gly Ile Lys Leu Lys Gly

420 425 430

Lys Pro Tyr Lys Thr Val Val Arg Gly Asn Thr Val Tyr Glu Asp Gly

435 440 445

Lys Gly Ile Val Gly Lys Pro Gly Tyr Gly Gln Phe Val Lys Arg Gln

450 455 460

Asn Asn Gln Lys Leu Ser Arg Thr Ile His Phe Thr Gly Asn Asp Phe

465 470 475 480

Arg Pro

<210> 41

<211> 555

<212> PRT

<213> Clostridium acidurici

<400> 41

Met Ser Tyr Asp Val Ile Ile Lys Asn Ala Lys Ile Pro Gln Gly Asp

1 5 10 15

Asn Thr Val Leu Thr Asn Ile Leu Val Lys Asp Glu Lys Ile Ala Gly

20 25 30

Phe Val Ser Thr Ile His Gly Ile Glu Ala Lys Glu Thr Ile Asp Ala

35 40 45

Glu Gly His Leu Thr Phe Pro Gly Phe Ile Asp Ser His Thr His Ile

50 55 60

Met Tyr His Gly Phe Gly His Arg Glu Asn Trp Val Thr Gly Thr Ser

65 70 75 80

Ala Ala Ala Arg Gly Gly Val Ser Thr Ile Ile Asp Met Pro Cys Cys

85 90 95

Thr Val Pro Ser Thr Arg Thr Val Lys Gln Leu Glu Leu Lys Ile Asp

100 105 110

Ile Ala Asn Ala Gln Thr Ile Val Asp Tyr Gly Leu Trp Gly Gly Val

115 120 125

Thr Gly Glu Asp Val Arg Glu Gly Trp Met His Asn Val Lys Glu Gln

130 135 140

Ala Asp Phe Gly Val Val Ala Phe Lys Val Tyr Met Thr Pro Ser Val

145 150 155 160

Pro Thr Tyr Pro Arg Val Thr Asp Pro Glu Met Leu Glu Cys Phe Lys

165 170 175

Glu Val Ala Lys Thr Gly Leu Pro Ile Gly Ile His Ala Glu Asn Phe

180 185 190

Ala Ile Cys Asp Phe Tyr Val Lys Lys Phe Gln Glu Glu Gly Arg Met

195 200 205

Asp Gly Pro Ala Trp Ser Glu Ala Arg Asn Glu Leu Ala Glu Lys Val

210 215 220

Ala Ile Glu Leu Gly Ile Ser Phe Ala Glu Ala Thr Gly Ala Arg Leu

225 230 235 240

His Ile Val His Met Ser Thr Gly Ile Gly Ala Lys Leu Ile Gly Glu

245 250 255

Ala Lys Lys Arg Gly Ile Asn Ala Thr Ser Glu Ser Cys Pro His Tyr

260 265 270

Leu Thr Leu Asn Tyr Gln Glu Ala Met Thr Lys His Gly Ser Gln Ala

275 280 285

Lys Ile Ala Pro Pro Ile Arg Thr Ser Ala Asp Asn Glu Glu Gln Trp

290 295 300

Glu Gly Leu Arg Asn Gly Ser Ile Asp Phe Met Ala Thr Asp His Ala

305 310 315 320

Pro Tyr Glu Ile Glu Ser Glu Lys Asn Lys Glu Gly Met Asn Ile Trp

325 330 335

Thr Ala Phe Pro Gly Ile Pro Gly Val Glu Thr Met Ala Pro Ile Ile

340 345 350

Ile Ser Glu Gly Leu Asn Lys Gly Arg Leu Ser Leu Ser Arg Thr Val

355 360 365

Glu Val Leu Ser Thr Asn Ala Ala Lys His Tyr Gly Leu Tyr Pro Lys

370 375 380

Lys Gly Ala Met Glu Ile Gly Ser Asp Ala Asp Phe Ala Ile Val Asp

385 390 395 400

Leu Glu Lys Lys Trp Thr Ile Asp Ala Lys Gly Asn Gln Ala Ser Met

405 410 415

Cys Gly Tyr Thr Pro Leu Asp Gly Met Glu Leu Lys Gly Arg Val Leu

420 425 430

Lys Thr Ile Val Arg Gly Thr Val Val Phe Asp Asp Thr Gln Glu Gly

435 440 445

Val Leu Pro Thr Leu Thr Asp Ala Glu Leu Glu Lys Thr Val His Asn

450 455 460

Phe Pro Glu Gly Thr Glu Glu Lys Phe Ala Asp Ile Phe Glu Gln Phe

465 470 475 480

Pro Glu Leu Ala Ala Ala Glu Tyr Asp Arg Ser Tyr Arg Lys Leu His

485 490 495

Pro Glu Lys Ile Asn Glu Asp Val Arg Arg Ile Lys Gly Ile Met Val

500 505 510

Lys Pro Gly Phe Gly Lys Tyr Val Lys Arg Gln Ser Ile Gln Val Leu

515 520 525

Pro Lys Thr Ile Thr Tyr Gly Thr Thr Thr Pro Thr Gly Val Asn Arg

530 535 540

Arg Glu Arg Glu Ser Leu Pro Glu Phe Asn Lys

545 550 555

<210> 42

<211> 515

<212> PRT

<213> Romboutsia lituseburensis

<400> 42

Met Val Asp Val Ile Ile Lys Asn Ala Arg Ile Pro Gln Gly Asp Asn

1 5 10 15

Thr Ile Val Thr Asn Ile Leu Val Lys Asn Glu Lys Ile Ala Gly Phe

20 25 30

Val Asp Asp Ile Ser Met Ile Asp Ala Lys Glu Val Ile Asp Ala Asn

35 40 45

Glu Asn Leu Thr Ile Pro Gly Cys Ile Asp Ser His Thr His Phe Met

50 55 60

Tyr Gln Gly Phe Pro His Arg Glu Asn Phe Leu Thr Gly Thr Ala Ala

65 70 75 80

Ala Ala Thr Gly Gly Val Thr Thr Ile Ile Asp Met Pro Cys Cys Ser

85 90 95

Val Pro Ser Val Arg Ser Ile Glu Gln Leu Glu Thr Lys Leu Ser Leu

100 105 110

Cys Gly Pro Gln Ala Val Val Asp Tyr Ala Met Trp Gly Gly Val Thr

115 120 125

Gly Glu Asp Val Arg Glu Gly Trp Met His Asn Val Lys Glu Gln Ala

130 135 140

Asp Tyr Gly Val Val Ala Phe Lys Val Tyr Met Thr Pro Ser Val Pro

145 150 155 160

Thr Tyr Pro Arg Val Thr Asp Pro Glu Met Tyr Glu Cys Phe Lys Ala

165 170 175

Val Ser Lys Thr Gly Leu Pro Ile Gly Ile His Ala Glu Asn Phe Ala

180 185 190

Met Cys Asp Phe Tyr Val Asn Lys Phe Lys Asn Glu Gly Arg Leu Asp

195 200 205

Gly Pro Ala Trp Ala Glu Ala Arg Met Glu Leu Ala Glu Lys Val Ala

210 215 220

Ile Glu Leu Gly Ile Ser Phe Ala Glu Ala Ser Gly Ala Arg Leu His

225 230 235 240

Ile Val His Met Ser Thr Gly Ile Gly Ala Lys Leu Val Lys Asp Ala

245 250 255

Lys Met Arg Gly Leu Asp Ile Thr Ser Glu Ser Cys Pro His Tyr Leu

260 265 270

Thr Leu Asn Tyr Gln Glu Ser Met Ser Lys Tyr Gly Pro Leu Ala Lys

275 280 285

Ile Ala Pro Pro Leu Arg Thr Lys Lys Asp Asn Glu Glu His Trp Gln

290 295 300

Gly Ile Asn Asp Gly Ser Ile Asp Phe Ile Ala Thr Asp His Ala Pro

305 310 315 320

Tyr Glu Ile Glu Ser Glu Lys His Lys Glu Gly Met Asn Ile Trp Thr

325 330 335

Ser Phe Pro Gly Ile Pro Gly Val Glu Thr Met Val Pro Val Val Val

340 345 350

Ser Glu Gly Tyr Asn Lys Gly Arg Ile Ser Leu Ser Lys Leu Val Asp

355 360 365

Ile Leu Ser Thr Asn Ala Ala Lys His Tyr Gly Leu Tyr Pro Lys Lys

370 375 380

Gly Ala Leu Asn Ile Gly Ser Asp Ala Asp Phe Thr Ile Ile Asp Leu

385 390 395 400

Glu Lys Glu Trp Thr Ile Asp Pro Lys Thr Met Val Ser Met Cys Gly

405 410 415

Tyr Thr Pro Leu Glu Gly Met Lys Leu Lys Gly Lys Val Phe Lys Thr

420 425 430

Val Val Arg Gly Asn Leu Val Tyr Gln Asp Gly Glu Asn Gly Thr Leu

435 440 445

Gly Ser Leu Asp Asn Tyr Asp Val Val Phe Asn Asn Pro Glu Glu Leu

450 455 460

Thr Ser Gly Glu Tyr Lys Val Ser Tyr Arg Lys Glu His Pro Glu Val

465 470 475 480

Ile Leu Asn His Val Lys Asn Ile Lys Gly Ile Met Val Lys Pro Gly

485 490 495

Phe Gly Lys Phe Thr Lys Arg Gln Gln Ile Met Lys Leu Asp Lys Lys

500 505 510

Ile Lys Phe

515

<210> 43

<211> 514

<212> PRT

<213> Paraclostridium bifermentans

<400> 43

Met Asp Leu Ile Ile Lys Asn Ala Asn Ile Pro Gln Gly Asp Glu Met

1 5 10 15

Val Leu Thr Asn Ile Leu Val Lys Asp Glu Lys Ile Val Gly Phe Val

20 25 30

Asp Asp Ile Asn Met Ile Asp Ala Lys Glu Ile Ile Asp Ala Lys Glu

35 40 45

Asn Leu Val Met Pro Gly Cys Ile Asp Ser His Thr His Phe Met Tyr

50 55 60

Gln Gly Phe Pro His Arg Glu Asn Phe Leu Thr Gly Thr Ala Ala Ala

65 70 75 80

Ala Thr Gly Gly Val Thr Thr Ile Ile Asp Met Pro Cys Cys Ser Val

85 90 95

Pro Ser Val Arg Ser Thr Glu Gln Leu Glu Thr Lys Leu Gly Ile Cys

100 105 110

Gly Pro Gln Ala Val Val Asp Tyr Ala Met Trp Gly Gly Val Thr Gly

115 120 125

Glu Asp Val Arg Glu Gly Trp Met His Asn Val Lys Glu Gln Ala Asp

130 135 140

Tyr Gly Val Val Ala Phe Lys Val Tyr Met Thr Pro Ser Val Pro Thr

145 150 155 160

Tyr Pro Arg Val Thr Asp Pro Glu Met Phe Glu Cys Phe Lys Ala Val

165 170 175

Ser Glu Thr Gly Leu Pro Ile Gly Ile His Ala Glu Asn Phe Ala Met

180 185 190

Cys Asp Phe Tyr Val Asn Lys Phe Lys Glu Glu Gly Arg Leu Asp Gly

195 200 205

Pro Ala Trp Ala Glu Ala Arg Met Glu Leu Ala Glu Lys Val Ala Ile

210 215 220

Glu Leu Gly Ile Ser Phe Ala Glu Ala Ser Lys Ala Arg Leu His Ile

225 230 235 240

Val His Met Ser Thr Gly Val Gly Ala Lys Leu Val Lys Glu Ala Lys

245 250 255

Met Arg Gly Leu Asp Val Thr Ser Glu Ser Cys Pro His Tyr Leu Thr

260 265 270

Leu Asn Tyr Gln Asp Ala Met Thr Glu Tyr Gly Pro Leu Ala Lys Ile

275 280 285

Ala Pro Pro Leu Arg Thr Lys Lys Asp Asn Glu Glu His Trp Lys Gly

290 295 300

Ile Asn Asp Gly Ser Ile Asp Phe Ile Ala Thr Asp His Ala Pro Tyr

305 310 315 320

Glu Ile Glu Ser Glu Lys Leu Lys Glu Gly Thr Asn Ile Trp Thr Ser

325 330 335

Phe Pro Gly Ile Pro Gly Val Glu Thr Met Val Pro Val Ile Val Ser

340 345 350

Glu Gly Tyr Asn Lys Gly Arg Ile Ser Leu Ser Lys Leu Val Asp Ile

355 360 365

Leu Ser Thr Asn Ala Ala Lys His Tyr Gly Leu Tyr Pro Lys Lys Gly

370 375 380

Ala Leu Asn Ile Gly Ser Asp Ala Asp Phe Ala Ile Ile Asp Leu Asn

385 390 395 400

Lys Glu Trp Thr Ile Lys Asn Glu Asn Leu Val Thr Met Cys Gly Tyr

405 410 415

Thr Pro Leu Glu Gly Met Lys Leu Lys Gly Arg Ile Val Lys Thr Ile

420 425 430

Val Arg Gly Asn Leu Val Tyr Glu Asp Gly Glu Ala Gly Val Leu Gly

435 440 445

Asn Leu Glu Asn Tyr Asp Val Leu Phe Asn Asn Pro Glu Glu Leu Thr

450 455 460

Ser Gly Glu Tyr Lys Val Ser Tyr Arg Lys Asp His Pro Glu Leu Ile

465 470 475 480

Leu Glu His Val Lys Asn Ile Lys Gly Ile Lys Val Lys Pro Gly Phe

485 490 495

Gly Lys Phe Ile Lys Arg Gln Thr Ile Ser Lys Leu Asp Lys Asn Ile

500 505 510

Lys Phe

<210> 44

<211> 486

<212> PRT

<213> Bacillus praedii

<400> 44

Met Asp Leu Ile Leu Lys Asn Ala Asn Ile Pro Gln Gly Asp Arg Gln

1 5 10 15

Val Leu Thr Asn Ile Leu Val Asn Glu Gly Lys Ile Val Gly Tyr Ser

20 25 30

Asn Asp Ile Ser Phe Leu Asn Ala Glu Lys Val Ile Asp Ile Gly Gly

35 40 45

Lys Leu Val Val Pro Gly Cys Ile Asp Pro His Thr His Phe Met Asp

50 55 60

Pro Gly Phe Thr His Arg Glu Thr Phe Ala Thr Gly Ser Arg Ser Ala

65 70 75 80

Ile Ala Gly Gly Leu Thr Thr Ile Ile Asp Met Pro Cys Cys Ser Lys

85 90 95

Pro Ser Val Arg Asp Gly Glu Ser Phe Gln Lys Lys Ile Gly Pro Ile

100 105 110

Arg Asp Gln Ala Tyr Val Asp Tyr Cys Phe Trp Gly Gly Met Thr Gly

115 120 125

Glu Asp Ala Arg Glu Gly Trp Leu Asp Asn Val Tyr Pro Gln Ile Glu

130 135 140

Glu Gly Val Val Ala Phe Lys Val Tyr Met Thr Pro Ser Val Pro Thr

145 150 155 160

Phe Pro Arg Val Ser Asp Ala Glu Met Leu Glu Ile Phe Lys Ala Val

165 170 175

Ser Lys Thr Gly Leu Pro Ile Gly Val His Ala Glu Asn Tyr Asp Ile

180 185 190

Cys Thr Phe Asn Ser Lys Lys Leu Glu Lys Glu Gly Arg Phe Asp Gly

195 200 205

Pro Ala Trp Ala Glu Ala Arg Ser Thr Leu Ala Glu Lys Val Ala Ile

210 215 220

Glu Leu Ile Ile Ser Phe Ala Glu Ala Thr Asp Ala Arg Val His Val

225 230 235 240

Val His Met Thr Thr Lys Glu Gly Ala Glu Leu Val Lys Ala Ala Lys

245 250 255

Lys Arg Gly Val Lys Val Thr Ala Glu Thr Cys Pro His Tyr Leu Val

260 265 270

Leu Asn Ala Lys Asp Ser Met Ser Glu Arg Gly Ser Phe Ala Lys Ile

275 280 285

Ala Pro Pro Leu Arg Gly Lys Glu Asp Asn Glu Ala His Trp Gln Ala

290 295 300

Leu Ala Asp Gly Thr Ile Asp Phe Val Gly Thr Asp His Ala Pro Tyr

305 310 315 320

Glu Ile Ala Thr Glu Lys Asp Phe Pro Gly Ala Thr Ile Trp Asn Thr

325 330 335

Phe Pro Gly Ile Pro Gly Val Glu Thr Met Val Pro Ile Leu Val Ser

340 345 350

Glu Gly Leu Asn Lys Gly Arg Leu Ser Leu Ser Arg Phe Val Glu Val

355 360 365

Thr Ser Arg Asn Ala Ala Ile His Tyr Gly Ile Tyr Pro Lys Lys Gly

370 375 380

Ser Met Glu Ile Gly Ser Asp Ala Asp Phe Thr Val Ile Asp Leu Glu

385 390 395 400

Lys Glu Tyr Glu Ile Asp Glu Gln Lys Thr Glu Ser Met Ala Lys Tyr

405 410 415

Asn Pro Leu His Gly Met Lys Leu Lys Gly Lys Pro Val Gln Thr Ile

420 425 430

Ile Arg Gly Thr Val Val Tyr Glu Asp Asp Lys Gly Val Val Gly Gln

435 440 445

Ala Gly Tyr Gly Gln Tyr Val Pro Arg Gln Ser Ile Gln Arg Leu Asp

450 455 460

Arg Thr Phe Lys Tyr Glu Lys Phe Glu Ala Ala Lys Val Glu Ala Lys

465 470 475 480

Glu Thr Val Lys Gln Asn

485

<210> 45

<211> 471

<212> PRT

<213> Carbydothermus islandicus

<400> 45

Met Gly Phe Asp Leu Val Leu Lys Asn Ala Lys Ile Pro Gln Gly Asn

1 5 10 15

Arg Thr Val Asp Thr His Ile Leu Val Lys Asp Gly Lys Ile Ala Gly

20 25 30

Phe Thr Asn Asp Leu Thr Gly Ile Glu Val Ala Glu Val Ile Asp Ala

35 40 45

Glu Asn Asn Leu Val Leu Pro Gly Cys Ile Asp Ser His Thr His Phe

50 55 60

Asn Asp Pro Gly Phe Thr His Arg Glu Asn Phe Ala Thr Gly Thr Gln

65 70 75 80

Ala Ala Ala Ala Gly Gly Val Thr Thr Ile Ile Asp Met Pro Cys Cys

85 90 95

Ser Val Pro Ser Val Arg Ser Val Asp Asn Leu Glu Ala Lys Leu Glu

100 105 110

Val Ile Lys Asp Lys Ala Tyr Val Asp Tyr Ala Met Trp Gly Gly Ala

115 120 125

Thr Gly Glu Asp Val Arg Asn Asn Ala Leu Tyr Asn Ile Lys Glu Gln

130 135 140

Ala Asp Tyr Gly Val Val Ala Phe Lys Val Tyr Met Thr Pro Ser Val

145 150 155 160

Pro Thr Tyr Pro Pro Ala Ser Asp Ala Glu Met Leu Glu Ile Phe Arg

165 170 175

Glu Val Ala Lys Thr Gly Leu Pro Val Gly Ile His Ala Glu Asn Leu

180 185 190

Asp Leu Cys Thr Tyr His Val Asn Lys Leu Arg Lys Glu Gly Arg Phe

195 200 205

Asp Gly Pro Ala Trp Ala Glu Ala Arg Leu Glu Leu Ala Glu Lys Val

210 215 220

Ala Ile Gln Met Ala Ile Ser Phe Ala Glu Glu Thr Gly Ala Arg Leu

225 230 235 240

His Ile Val His Met Ser Thr Gly Ile Gly Gly Val Leu Val Gly Glu

245 250 255

Ala Lys Lys Arg Gly Ile Asp Val Thr Ala Glu Thr Cys Pro His Tyr

260 265 270

Leu Val Leu Asn Ala Tyr Glu Ser Met Thr Lys Trp Arg Gln Phe Ala

275 280 285

Lys Ile Ala Pro Pro Leu Arg Thr Thr Lys Asp Asn Glu Met Leu Trp

290 295 300

Glu Gln Leu Ala Asp Gly Arg Val Asp Phe Ile Ala Thr Asp His Ala

305 310 315 320

Pro Tyr Glu Ile Ala Thr Glu Lys Glu Ala Glu Gly Met Asp Ile Trp

325 330 335

Thr Ala Phe Pro Gly Ile Pro Gly Val Glu Thr Met Val Pro Ile Ile

340 345 350

Val Ser Glu Gly Tyr Asn Lys Gly Arg Leu Ser Leu Ser Arg Leu Val

355 360 365

Asp Val Leu Ser Arg Asn Ala Ala Ile His Tyr Gly Leu Tyr Pro Lys

370 375 380

Lys Gly Ala Leu Glu Ile Gly Ser Asp Ala Asp Phe Thr Val Ile Asp

385 390 395 400

Leu Asp Tyr Glu Trp Thr Ile Asp Lys Asp Lys Met Tyr Thr Met Ala

405 410 415

Lys Tyr Thr Pro Leu His Gly Met Lys Leu Lys Gly Lys Pro Val Lys

420 425 430

Thr Ile Val Arg Gly Lys Leu Val Tyr Asp Asp Lys Asn Gly Ile Val

435 440 445

Gly Lys Pro Gly Tyr Gly Gln Phe Val Lys Arg Gln Thr Ile Ser Lys

450 455 460

Leu Glu Arg Trp Leu Lys Phe

465 470

<210> 46

<211> 471

<212> PRT

<213> Caloramator australicus

<400> 46

Met Arg Tyr Asp Val Ile Val Lys Asn Ala Arg Ile Pro Lys Gly Asp

1 5 10 15

Asp Thr Val Leu Thr Asn Ile Leu Val Lys Asp Glu Lys Ile Ala Gly

20 25 30

Phe Ile Asp Asn Leu Glu Gly Ile Glu Ala Asp Thr Ile Ile Asp Ala

35 40 45

Glu Gly His Leu Thr Leu Pro Gly Cys Ile Asp Ser His Thr His Tyr

50 55 60

Met Asp Pro Gly Phe Thr His Arg Glu Asn Phe Leu Thr Gly Thr Ser

65 70 75 80

Gly Ala Ala Val Gly Gly Val Thr Thr Ile Ile Asp Met Pro Cys Cys

85 90 95

Ser Val Pro Ser Val Arg Gly Val Ala Glu Leu His Asn Lys Ile Asn

100 105 110

Ala Ile Lys Asp Lys Ala Ile Val Asp Tyr Ala Met Trp Gly Gly Val

115 120 125

Thr Gly Glu Asp Val Arg Asn Lys Lys Leu Asn Ile Val Glu Glu Gln

130 135 140

Ala Ala Glu Gly Val Val Ala Phe Lys Ile Tyr Met Thr Pro Ser Val

145 150 155 160

Pro Thr Tyr Pro Arg Val Thr Asp Pro Glu Met Leu Glu Ile Phe Arg

165 170 175

Ala Val Ala Lys Thr Gly Leu Pro Val Gly Val His Ala Glu Asn Phe

180 185 190

Ala Ile Cys Asp Tyr Met Val Lys Lys Phe Gln Glu Glu Gly Arg Met

195 200 205

Asp Gly Pro Ala Trp Ala Glu Ala Arg Met Glu Leu Ala Glu Lys Val

210 215 220

Ala Ile Gln Leu Cys Ile Ser Tyr Ala Glu Glu Thr Gly Ala Arg Phe

225 230 235 240

His Val Val His Met Ser Thr Gly Ile Gly Ala Lys Leu Val Lys Glu

245 250 255

Ala Lys Met Lys Gly Leu Asp Val Thr Ser Glu Thr Cys Pro His Tyr

260 265 270

Leu Thr Leu Asn Tyr Gln Glu His Met Thr Lys Phe Lys Gln Phe Val

275 280 285

Lys Ile Ala Pro Pro Ile Arg Thr Lys Lys Asp Asn Glu Glu Leu Trp

290 295 300

Gln Gly Leu Gln Asp Gly Ser Val Asp Phe Ile Ala Thr Asp His Ala

305 310 315 320

Pro Tyr Glu Ile Pro Thr Glu Lys Asp Ala Pro Gly Met Thr Ile Trp

325 330 335

Thr Ala Phe Pro Gly Ile Pro Gly Thr Glu Thr Met Val Pro Ile Ile

340 345 350

Val Ser Glu Gly Tyr Asn Lys Gly Arg Ile Ser Leu Ser Arg Leu Val

355 360 365

Glu Ile Leu Ser Thr Asn Ala Ala Lys His Tyr Gly Leu Tyr Pro Lys

370 375 380

Lys Gly Thr Leu Met Ile Gly Ser Asp Ala Asp Phe Thr Ile Ile Asp

385 390 395 400

Leu Asn Lys Glu Trp Val Ile Glu Lys Glu Lys Met Gln Cys Leu Asn

405 410 415

Lys Tyr Thr Pro Phe Asp Gly Met Lys Leu Lys Gly Lys Val Val Lys

420 425 430

Thr Ile Val Arg Gly Thr Leu Val Tyr Asp Asp Glu Lys Gly Ile Val

435 440 445

Gly Lys Pro Gly Tyr Gly Gln Trp Ile Lys Arg Gln Ser Ile Gln Lys

450 455 460

Leu Asp Arg Val Ile Lys Phe

465 470

<210> 47

<211> 514

<212> PRT

<213> Paraclostridium bifermentans

<400> 47

Met Asp Leu Ile Ile Lys Asn Ala Asn Ile Pro Gln Gly Asp Glu Met

1 5 10 15

Val Leu Thr Asn Ile Leu Val Lys Asp Glu Lys Ile Val Gly Phe Val

20 25 30

Asp Asp Ile Asn Met Ile Asp Ala Lys Glu Ile Ile Asp Ala Asn Lys

35 40 45

Asn Leu Val Met Pro Gly Cys Ile Asp Ser His Thr His Phe Met Tyr

50 55 60

Gln Gly Phe Pro His Arg Glu Asn Phe Leu Thr Gly Thr Ala Ala Ala

65 70 75 80

Ala Thr Gly Gly Val Thr Thr Ile Ile Asp Met Pro Cys Cys Ser Val

85 90 95

Pro Ser Val Arg Ser Ile Glu Gln Leu Glu Thr Lys Leu Gly Ile Cys

100 105 110

Gly Pro Gln Ala Val Val Asp Tyr Ala Met Trp Gly Gly Val Thr Gly

115 120 125

Glu Asp Val Arg Glu Gly Trp Met His Asn Val Lys Glu Gln Ala Asp

130 135 140

Tyr Gly Val Val Ala Phe Lys Val Tyr Met Thr Pro Ser Val Pro Thr

145 150 155 160

Tyr Pro Arg Val Thr Asp Pro Glu Met Phe Glu Cys Phe Lys Ala Val

165 170 175

Ser Glu Thr Gly Leu Pro Ile Gly Ile His Ala Glu Asn Phe Ala Met

180 185 190

Cys Asp Phe Tyr Val Asn Lys Phe Lys Glu Glu Gly Arg Leu Asp Gly

195 200 205

Pro Ala Trp Ala Glu Ala Arg Met Glu Leu Ala Glu Lys Val Ala Ile

210 215 220

Glu Leu Gly Ile Ser Phe Ala Glu Ala Ser Lys Ala Arg Leu His Ile

225 230 235 240

Val His Met Ser Thr Gly Ile Gly Ala Lys Leu Val Lys Glu Ala Lys

245 250 255

Met Arg Gly Leu Asp Val Thr Ser Glu Ser Cys Pro His Tyr Leu Thr

260 265 270

Leu Asn Tyr Gln Asp Ala Met Thr Glu Tyr Gly Pro Leu Ala Lys Ile

275 280 285

Ala Pro Pro Leu Arg Thr Lys Lys Asp Asn Glu Glu His Trp Lys Gly

290 295 300

Ile Asn Asp Gly Ser Ile Asp Phe Ile Ala Thr Asp His Ala Pro Tyr

305 310 315 320

Glu Ile Glu Ser Glu Lys Leu Lys Glu Gly Thr Asn Ile Trp Thr Ser

325 330 335

Phe Pro Gly Ile Pro Gly Val Glu Thr Met Val Pro Val Ile Val Ser

340 345 350

Glu Gly Tyr Asn Lys Gly Lys Ile Ser Leu Ser Lys Leu Val Asp Ile

355 360 365

Leu Ser Thr Asn Ala Ala Lys His Tyr Gly Leu Tyr Pro Lys Lys Gly

370 375 380

Ala Leu Asn Ile Gly Ser Asp Ala Asp Phe Ala Ile Ile Asp Leu Asn

385 390 395 400

Lys Glu Trp Thr Ile Lys Asn Glu Asn Leu Val Thr Met Cys Gly Tyr

405 410 415

Thr Pro Leu Glu Gly Met Lys Leu Lys Gly Arg Ile Ile Lys Thr Ile

420 425 430

Val Arg Gly Asn Leu Val Tyr Glu Asp Gly Glu Ala Gly Val Leu Gly

435 440 445

Lys Leu Glu Asn Tyr Asp Val Val Phe Asn Asn Pro Glu Glu Leu Thr

450 455 460

Ser Gly Glu Tyr Lys Val Ser Tyr Arg Lys Asp His Pro Glu Leu Ile

465 470 475 480

Leu Glu His Val Lys Asn Ile Lys Gly Ile Lys Val Lys Pro Gly Phe

485 490 495

Gly Lys Phe Ile Lys Arg Gln Thr Ile Ser Lys Leu Asp Lys Asn Ile

500 505 510

Lys Phe

<210> 48

<211> 533

<212> PRT

<213> Tepidimicrobium xylanilyticum

<400> 48

Met Val Tyr Asp Val Ile Ile Lys Asn Ala Arg Ile Pro Gln Gly Asp

1 5 10 15

Asp Thr Val Ile Thr Asn Ile Leu Val Lys Asp Glu Lys Ile Ala Gly

20 25 30

Phe Leu Asp Asn Val Val Gly Ile Glu Ala Lys Glu Val Ile Asp Ala

35 40 45

Asn Gly His Leu Thr Leu Pro Gly Cys Ile Asp Ser His Thr His Phe

50 55 60

Met Tyr Gln Gly Phe Pro His Arg Glu Asn Phe Leu Thr Gly Ser Ala

65 70 75 80

Ala Ala Ala Arg Gly Gly Ile Thr Thr Val Ile Asp Met Pro Cys Cys

85 90 95

Ser Val Pro Ser Ala Arg Ser Val Glu Gln Leu Lys Leu Lys Ile Glu

100 105 110

Leu Val Glu Pro Gln Ala Leu Val Asp Tyr Ala Met Trp Gly Gly Val

115 120 125

Thr Gly Glu Asp Val Arg Glu Gly Trp Leu His Asn Val Lys Glu Gln

130 135 140

Ala Asp Phe Gly Ala Val Ala Phe Lys Val Tyr Met Thr Pro Ser Val

145 150 155 160

Pro Thr Tyr Pro Arg Val Ser Asp Pro Glu Met Tyr Glu Ala Phe Lys

165 170 175

Ala Val Ala Glu Thr Gly Leu Pro Ile Gly Ile His Ala Glu Asn Phe

180 185 190

Ala Met Cys Asp Tyr Phe Val Lys Lys Phe Gln Arg Glu Gly Arg Met

195 200 205

Asp Gly Pro Ala Trp Ala Glu Ala Arg Met Glu Leu Ala Glu Lys Val

210 215 220

Ala Ile Gln Leu Gly Ile Ser Phe Ala Glu Asp Thr Gly Ala Arg Leu

225 230 235 240

His Ile Val His Met Ser Thr Gly Ile Gly Ala Lys Leu Val Gly Glu

245 250 255

Ala Lys Ala Arg Gly Leu Asn Val Thr Ser Glu Thr Cys Pro His Tyr

260 265 270

Leu Thr Leu Asn Tyr Gln Glu Ala Met Thr Lys Tyr Gly Pro Leu Ala

275 280 285

Lys Ile Ala Pro Pro Leu Arg Glu Lys Lys Asp Asn Glu Met Leu Trp

290 295 300

Glu Gly Leu Asn Asn Gly Ser Val Asp Phe Ile Ala Thr Asp His Ala

305 310 315 320

Pro Tyr Glu Ile Glu Ser Glu Lys His Lys Lys Gly Met Asn Ile Trp

325 330 335

Thr Ala Phe Pro Gly Ile Pro Gly Val Glu Thr Met Val Pro Ile Ile

340 345 350

Val Ser Glu Gly Tyr Asn Lys Gly Arg Ile Ser Leu Ser Lys Met Val

355 360 365

Asp Ile Leu Ser Thr Asn Ala Ala Lys His Tyr Gly Leu Tyr Pro Lys

370 375 380

Lys Gly Ala Met His Ile Gly Ser Asp Ala Asp Phe Thr Ile Ile Asp

385 390 395 400

Leu Glu Lys Arg Trp Thr Ile Asp Leu Lys Asp Gln Ala Ser Met Cys

405 410 415

Gly Tyr Thr Pro Leu Glu Gly Met Glu Leu Lys Gly Lys Val Val Lys

420 425 430

Thr Ile Val Arg Gly His Leu Val Phe Glu Asp Val Glu Gly Ser Thr

435 440 445

Leu Lys Asp Leu Ser Asp Glu Glu Leu Gln Arg Ile Val His Lys Tyr

450 455 460

Pro Glu Gly Val Glu Glu Arg Tyr Ser Glu Ile Phe Lys Glu Phe Pro

465 470 475 480

Asn Leu His Ser Gln Glu Tyr Glu Lys Ser Tyr Arg Lys Glu His Pro

485 490 495

Glu Leu Ile Asp Gln His Ile Arg Gly Ile Lys Gly Ile Lys Val Arg

500 505 510

Pro Gly Phe Gly Lys Phe Val Lys Arg Gln Ser Ile Gln Val Leu Pro

515 520 525

Arg Thr Ile Thr Tyr

530

<210> 49

<211> 490

<212> PRT

<213> 米氏解硫胺素芽孢杆菌(Aneurinibacillus migulanus)

<400> 49

Met Asp Leu Ile Leu Lys Asn Ala Asn Ile Pro Gln Gly Asp Arg Gln

1 5 10 15

Val Gln Thr Asn Ile Leu Val Glu Asn Gly Thr Ile Val Gly Phe Val

20 25 30

Asn Ser Ile Asn Gly Leu Gln Ala Lys Asn Val Ile Asp Leu Lys Gly

35 40 45

Lys Leu Val Val Pro Gly Cys Ile Asp Pro His Thr His Phe Met Asp

50 55 60

Pro Gly Phe Thr His Arg Glu Thr Phe Ala Thr Gly Thr Met Ser Ala

65 70 75 80

Ala Ala Gly Gly Leu Thr Thr Ile Ile Asp Met Pro Cys Cys Ser Lys

85 90 95

Pro Ser Val Arg Ser Glu Glu Ser Phe Tyr Ala Lys Ile Asp Pro Ile

100 105 110

Lys Asp Lys Ala Tyr Ile Asp Tyr Cys Phe Trp Gly Gly Met Thr Gly

115 120 125

Glu Asp Val Arg Glu Gly Leu Leu Asp Asn Val Trp Gly Gln Lys Glu

130 135 140

Ala Gly Val Val Ala Phe Lys Ser Tyr Met Thr Pro Ser Val Pro Thr

145 150 155 160

Phe Pro Lys Val Ser Asp Ala Glu Leu Leu Glu Ile Phe Tyr Lys Val

165 170 175

Ala Glu Thr Gly Leu Pro Val Gly Val His Ala Glu Asn Tyr Asp Ile

180 185 190

Cys Ser Phe Tyr Thr Glu Lys Leu Gln Lys Glu Gly Arg Leu Asp Gly

195 200 205

Pro Ala Trp Ala Glu Ala Arg Lys Ala Leu Ala Glu Lys Val Ala Ile

210 215 220

Glu Leu Ile Ile Ser Tyr Ala Glu Glu Thr Gly Ala Arg Ala His Ile

225 230 235 240

Val His Met Ser Thr Lys Glu Gly Ala His Leu Ile Arg Gln Ala Lys

245 250 255

Glu Arg Gly Val Lys Ile Thr Ala Glu Thr Cys Pro His Tyr Leu Val

260 265 270

Leu Asn Ala Gln Glu Ser Met Thr Lys Tyr Gly Thr Phe Ala Lys Ile

275 280 285

Ala Pro Pro Leu Arg Gly Val Glu Asp Asn Glu Ile His Trp Gln Ala

290 295 300

Leu Ala Asp Gly Thr Ile Asp Phe Val Gly Thr Asp His Ala Pro Tyr

305 310 315 320

Glu Ile Pro Thr Glu Lys Ala Ala Glu Asp Ser Asp Ile Trp Asn Ser

325 330 335

Phe Pro Gly Ile Pro Gly Val Glu Thr Met Val Pro Ile Leu Val Ser

340 345 350

Glu Gly Leu Asn Lys Gly Arg Leu Ser Leu Ser Arg Phe Val Glu Val

355 360 365

Thr Ser Arg Asn Ala Ala Ile His Tyr Gly Ile Tyr Pro Lys Lys Gly

370 375 380

Ala Ile Glu Ile Gly Ser Asp Ala Asp Phe Thr Val Ile Asp Leu Glu

385 390 395 400

Lys Glu His Val Ile Asp Gln Glu Gln Thr Tyr Ser Met Ala Lys Tyr

405 410 415

Asn Pro Leu His Asp Ile Lys Leu Lys Gly Met Pro Val Met Thr Ile

420 425 430

Val Arg Gly Asn Val Val Tyr Glu Asp Gly Lys Gly Ile Val Gly Gln

435 440 445

Glu Gly Tyr Gly Gln Tyr Val Lys Arg Gln Ser Thr Gln Gln Leu Asp

450 455 460

Ala Val Ile Asn Leu Thr Lys Ser Asn Glu Ser Lys Ala Val Ser Gln

465 470 475 480

Ser Ser Glu Lys Lys Glu Ser Val Lys Leu

485 490

<210> 50

<211> 497

<212> PRT

<213> 芽孢杆菌细菌(Bacillus bacterium)

<400> 50

Met Asp Leu Ile Leu Lys Asn Ala Leu Ile Pro Gln Gly Asp Arg Lys

1 5 10 15

Val Lys Thr Asn Ile Leu Val Asp Asn Gly Lys Ile Val Gly Phe Thr

20 25 30

Ser Ala Leu Asn Gly Leu Ser Ala Asp Arg Val Ile Asp Ile Lys Glu

35 40 45

Asn Leu Val Val Pro Gly Cys Ile Asp Pro His Thr His Phe Met Asp

50 55 60

Pro Gly Phe Thr His Arg Glu Thr Phe Thr Thr Gly Thr Arg Ser Ala

65 70 75 80

Ala Ala Gly Gly Val Thr Thr His Ile Asp Met Pro Cys Cys Ser Lys

85 90 95

Pro Ser Val Arg Asp Ala Glu Ser Phe His Lys Lys Leu Ala Pro Leu

100 105 110

Lys Asp Gln Ala Tyr Ile Asp Phe Ala Phe Trp Gly Gly Met Thr Gly

115 120 125

Glu Asp Val Arg Glu Gly Leu Leu Asp Asn Ile His Pro Gln Ile Asp

130 135 140

Glu Gly Val Val Ala Phe Lys Val Tyr Met Thr Pro Ser Val Pro Thr

145 150 155 160

Phe Pro Lys Val Ser Asp Ala Glu Met Leu Glu Ile Phe Ser His Ile

165 170 175

Ala Pro Thr Gly Met Pro Ile Gly Val His Ala Glu Asn Tyr Asp Ile

180 185 190

Cys Thr Phe Tyr Ser Glu Lys Leu Lys Lys Glu Gly Arg Leu Asp Gly

195 200 205

Pro Ala Trp Ala Glu Ala Arg Met Ala Leu Ala Glu Lys Thr Ala Ile

210 215 220

Gln Leu Ile Ile Ser Phe Ala Glu Ala Thr Gly Ala Arg Val His Val

225 230 235 240

Val His Met Ser Thr Lys Glu Gly Val Glu Leu Val Arg Ala Ala Lys

245 250 255

Lys Arg Gly Val Lys Val Thr Ala Glu Thr Cys Pro His Tyr Leu Met

260 265 270

Leu Asn Ala Glu Asp Ser Met Ser Glu Arg Gly Thr Phe Ala Lys Ile

275 280 285

Ala Pro Pro Leu Arg Glu Lys Glu Asp Asn Ile Val His Trp Gln Gly

290 295 300

Leu Ala Asp Gly Thr Ile Asp Phe Val Gly Thr Asp His Ala Pro Tyr

305 310 315 320

Glu Ile Ala Thr Glu Lys Asp Ala Pro Asp Ser Thr Ile Trp Asn Ser

325 330 335

Phe Pro Gly Ile Pro Gly Val Glu Thr Met Val Pro Ile Leu Val Ser

340 345 350

Glu Gly Leu Asn Lys Gly Arg Leu Ser Leu Ser Arg Phe Val Glu Val

355 360 365

Thr Ser Arg Asn Ala Ala Ile His Phe Gly Ile Tyr Pro Lys Lys Gly

370 375 380

Ala Leu Glu Ile Gly Ser Asp Ala Asp Phe Thr Val Ile Asp Leu Glu

385 390 395 400

Arg Glu Tyr Thr Ile Asp Glu Gln Lys Thr Glu Ser Met Ala Lys Tyr

405 410 415

Asn Pro Leu His Gly Leu Gln Leu Lys Gly Lys Pro Ile Gln Thr Ile

420 425 430

Val Arg Gly Arg Val Val Tyr Asp Glu Asp Asn Gly Gly Ile Val Gly

435 440 445

Glu Ala Gly Tyr Gly Gln Tyr Val Lys Arg Gln Ala Ile Gln Ser Leu

450 455 460

Glu Arg Val Ile Lys Tyr Glu Thr Tyr Glu Glu Gly Ala Lys Glu Ala

465 470 475 480

Glu Ala Lys Pro Arg Val Glu Lys Ala Leu Arg Arg Asp Leu Ile Thr

485 490 495

Asn

<210> 51

<211> 489

<212> PRT

<213> Bacillus notoginsengisoli

<400> 51

Met Asp Leu Ile Leu Lys Asn Ala Asn Ile Pro Gln Gly Asp Arg Gln

1 5 10 15

Val Leu Thr Asn Val Leu Val Asp Glu Gly Lys Ile Val Gly Phe Thr

20 25 30

Asn Asp Ile Asn Phe Leu His Ala Asp Glu Ile Ile Asp Ile Lys Gly

35 40 45

Lys Leu Val Val Pro Gly Cys Ile Asp Pro His Thr His Phe Met Asp

50 55 60

Pro Gly Phe Thr His Arg Glu Thr Phe Glu Thr Gly His Arg Ser Ala

65 70 75 80

Ala Ala Gly Gly Leu Thr Leu His Ile Asp Met Pro Cys Cys Ser Lys

85 90 95

Pro Ser Val Arg Asp Gly Glu Ser Phe Phe Lys Lys Leu Asn Pro Ile

100 105 110

Lys Glu Lys Ala Tyr Ile Asp Tyr Cys Phe Trp Gly Gly Met Thr Gly

115 120 125

Glu Asp Ala Arg Glu Gly Trp Leu Asp Asn Ile Tyr Pro Gln Ile Glu

130 135 140

Gln Gly Val Val Ala Phe Lys Val Tyr Met Thr Pro Ser Val Pro Thr

145 150 155 160

Phe Pro Lys Val Ser Asp Gly Glu Met Phe Glu Ile Phe Arg Lys Val

165 170 175

Ala Pro Thr Gly Leu Pro Ile Gly Val His Ala Glu Asn Tyr Asp Ile

180 185 190

Cys Thr Phe Asn Thr Glu Arg Leu Lys Asn Glu Gly Arg Thr Asp Gly

195 200 205

Pro Ala Trp Ala Glu Ala Arg Ser Ser Leu Ala Glu Lys Val Ala Ile

210 215 220

Glu Leu Ile Ile Ser Tyr Ala Glu Glu Val Asp Ala Arg Val His Val

225 230 235 240

Val His Met Ser Thr Lys Glu Gly Val Glu Leu Val Arg Arg Ala Lys

245 250 255

Glu Arg Gly Leu Lys Val Thr Ala Glu Thr Cys Pro His Tyr Leu Met

260 265 270

Leu Asn Ala Lys Asp Ala Met Thr Glu Arg Gly Thr Phe Ala Lys Ile

275 280 285

Ala Pro Pro Leu Arg Gly Lys Ala Asp Asn Glu Ala His Trp Gln Ala

290 295 300

Leu Ala Asp Gly Thr Ile Asp Phe Val Gly Thr Asp His Ala Pro Tyr

305 310 315 320

Glu Ile Pro Thr Glu Lys Ala Ala Pro Asp Ser Thr Ile Trp Asn Ser

325 330 335

Phe Pro Gly Ile Pro Gly Val Glu Thr Met Val Pro Val Leu Val Ser

340 345 350

Glu Gly Leu Asn Lys Gly Arg Leu Ser Leu Ser Arg Phe Val Glu Val

355 360 365

Thr Ser Thr Asn Ala Ala Lys His Tyr Gly Ile Tyr Pro Lys Lys Gly

370 375 380

Ala Met Glu Ile Gly Ser Asp Ala Asp Phe Thr Val Ile Asp Leu Asp

385 390 395 400

Arg Glu Tyr Glu Ile Asp Glu Gln Lys Thr Glu Ser Met Ala Lys Tyr

405 410 415

Asn Pro Leu His Gly Leu Gln Leu Lys Gly Lys Pro Val Gln Thr Ile

420 425 430

Val Arg Gly Lys Leu Ile Tyr Asp Glu Asp Asn Gly Gly Ile Val Gly

435 440 445

Glu Ala Gly Tyr Gly Gln Phe Val Pro Arg Gln Ser Ile Gln Arg Leu

450 455 460

Glu Arg Val Leu Lys Ile Glu Lys Tyr Glu Ala Ala Ala Val Thr Glu

465 470 475 480

Lys Ser Glu Lys Ala Glu Val Ile Asn

485

<210> 52

<211> 486

<212> PRT

<213> 海泥芽孢杆菌 (Bacillus oceanisediminis)

<400> 52

Met Asp Leu Ile Leu Lys Asn Ala Ser Ile Pro Gln Gly Asp Arg Gln

1 5 10 15

Val Leu Thr Asn Ile Leu Val Asn Glu Gly Lys Ile Val Gly Tyr Thr

20 25 30

Asn Asp Ile Ser Phe Leu Asn Ala Ala Arg Val Ile Asp Ile Lys Gly

35 40 45

Lys Leu Val Val Pro Gly Cys Ile Asp Pro His Thr His Phe Met Asp

50 55 60

Pro Gly Phe Thr His Arg Glu Thr Phe Ala Thr Gly Ser Arg Ser Ala

65 70 75 80

Ala Ala Gly Gly Leu Thr Thr Ile Ile Asp Met Pro Cys Cys Ser Lys

85 90 95

Pro Ser Val Arg Asp Gly Glu Ser Phe Asn Lys Lys Ile Gly Pro Ile

100 105 110

Arg Asp Gln Ala Tyr Ile Asp Tyr Cys Phe Trp Gly Gly Met Thr Gly

115 120 125

Glu Asp Ala Arg Glu Gly Trp Ile Asp Asn Ile Tyr Pro Gln Ile Asp

130 135 140

Gln Gly Val Val Ala Phe Lys Val Tyr Met Thr Pro Ser Val Pro Thr

145 150 155 160

Phe Pro Lys Val Ser Asp Ala Glu Met Leu Glu Ile Phe Ser Asn Val

165 170 175

Ala Lys Thr Gly Leu Pro Ile Gly Val His Ala Glu Asn Tyr Asp Ile

180 185 190

Cys Thr Phe Tyr Ser Lys Lys Leu Glu Lys Glu Gly Arg Leu Asp Gly

195 200 205

Pro Ala Trp Ala Glu Ala Arg Ser Ser Leu Ala Glu Lys Val Ala Ile

210 215 220

Glu Leu Ile Leu Ser Phe Ala Glu Ala Thr Asp Ala Arg Val His Val

225 230 235 240

Val His Met Ser Thr Lys Glu Gly Val Glu Leu Val Lys Ala Ala Lys

245 250 255

Lys Arg Gly Val Lys Val Thr Ala Glu Thr Cys Pro His Tyr Leu Val

260 265 270

Leu Asn Ala Lys Asp Ser Met Ser Glu Arg Gly Ser Tyr Ala Lys Ile

275 280 285

Ala Pro Pro Leu Arg Gly Lys Glu Asp Asn Glu Ser His Trp Gln Ala

290 295 300

Leu Ala Asp Gly Thr Ile Asp Phe Val Gly Thr Asp His Ala Pro Tyr

305 310 315 320

Glu Ile Ala Thr Glu Lys Asp Tyr Pro Gly Ala Thr Ile Trp Asn Thr

325 330 335

Phe Pro Gly Ile Pro Gly Val Glu Thr Met Val Pro Ile Leu Val Ser

340 345 350

Glu Gly Leu Asn Lys Gly Arg Leu Ser Leu Ser Arg Phe Val Glu Val

355 360 365

Thr Ser Arg Asn Ala Ala Ile His Tyr Gly Ile Tyr Pro Lys Lys Gly

370 375 380

Ser Met Glu Ile Gly Ser Asp Ala Asp Phe Thr Val Ile Asp Leu Glu

385 390 395 400

Lys Glu Tyr Val Ile Asp Glu Gln Lys Thr Glu Ser Met Ala Lys Tyr

405 410 415

Asn Pro Leu His Gly Met Lys Leu Lys Gly Lys Pro Val Gln Thr Ile

420 425 430

Ile Arg Gly Asn Val Ile Phe Asp Glu Asp Lys Gly Ile Val Gly Glu

435 440 445

Ala Gly Tyr Gly Glu Tyr Val Pro Arg Gln Ser Ile Gln Arg Leu Glu

450 455 460

Arg Thr Phe Lys Tyr Glu Lys Tyr Glu Ala Val Lys Ala Lys Thr Lys

465 470 475 480

Asp Pro Val Ala Gln Gln

485

<210> 53

<211> 471

<212> PRT

<213> 类芽孢杆菌 (Paenibacillus sp.)

<400> 53

Met Ser Tyr Asp Leu Ile Ile Lys Asn Ala Ser Ile Pro Gln Gly Asp

1 5 10 15

Arg Gln Val Leu Thr Asp Ile Leu Val Asn Lys Gly Val Ile Val Gly

20 25 30

Phe Ala Lys Asp Ile Glu Ala Asp Ala Ala Glu Ile Ile Asp Ala Gly

35 40 45

Gly Lys Leu Val Leu Pro Gly Val Ile Asp Ser His Thr His Phe Asn

50 55 60

Asp Pro Gly Phe Thr His Arg Glu Asp Phe Val Thr Gly Thr Ser Ser

65 70 75 80

Ala Ala Ala Gly Gly Val Thr Met Ile Val Asp Met Pro Cys Cys Ser

85 90 95

Val Pro Ser Val Arg Ser Ala Asp Asn Leu Phe Asn Lys Leu Asn Ala

100 105 110

Leu Glu Gly Lys Gly Ile Val Asp Tyr Ala Met Trp Gly Gly Ile Thr

115 120 125

Gly Glu Asp Val Arg Asn Asn Val Leu Pro Glu Ile Ile Ser Glu Gln

130 135 140

Ile Ala Asn Gly Val Val Ala Phe Lys Val Tyr Met Thr Pro Ser Val

145 150 155 160

Pro Ser Tyr Pro Arg Val Thr Asp Pro Glu Met Leu Glu Ala Phe Ala

165 170 175

Ala Val Ser Pro Thr Gly Leu Pro Ile Gly Ile His Ala Glu Asn Phe

180 185 190

Ala Ile Cys Asp Tyr Phe Thr Asn Lys Leu Lys Gly Glu Gly Arg Leu

195 200 205

Asp Tyr Pro Ala Trp Ala Glu Ala Arg Met Ala Leu Ala Glu Lys Ala

210 215 220

Ala Ile Gln Leu Gly Ile Ser Phe Ala Glu Glu Thr Gly Ala Arg Leu

225 230 235 240

His Ile Val His Met Ser Thr Gly Ala Gly Asn Glu Leu Ile Arg Glu

245 250 255

Ala Lys Leu Lys Gly Thr Lys Val Thr Ala Glu Ser Cys Pro His Tyr

260 265 270

Leu Thr Leu Asn Ala Val Asp Ala Met Thr Glu Phe Gly Thr Phe Ala

275 280 285

Lys Ile Ala Pro Pro Leu Arg Thr Ala Lys Asp Asn Glu Val Leu Trp

290 295 300

Gln Gly Leu Ala Asp Gly Thr Ile Asp Phe Met Ala Thr Asp His Ala

305 310 315 320

Pro Tyr Glu Ile Ala Thr Glu Lys Asp Ala Pro Gly Met Asp Val Trp

325 330 335

Thr Ser Phe Pro Gly Ile Pro Gly Val Glu Thr Leu Val Pro Ile Met

340 345 350

Ile Ser Glu Gly Tyr Asn Lys Gly Arg Leu Ser Leu Ser Arg Leu Val

355 360 365

Glu Leu Leu Cys Thr Asn Pro Ala Ile His Tyr Gly Leu Tyr Pro Lys

370 375 380

Lys Gly Ala Leu Glu Ile Gly Thr Asp Ala Asp Phe Thr Ile Val Asp

385 390 395 400

Leu Glu Lys Glu Trp Thr Leu Asp Lys Asp Gln Met His Cys Lys Pro

405 410 415

Lys Tyr Thr Pro Phe His Gly Met Lys Leu Lys Gly Lys Val Asp Lys

420 425 430

Thr Ile Val Arg Gly Thr Leu Val Tyr Asp Asp Ala Leu Gly Val Val

435 440 445

Gly Lys Pro Gly His Gly Glu Tyr Val Lys Arg Gln Thr Ile Ser Glu

450 455 460

Leu Pro Arg Leu Leu Lys Tyr

465 470

<210> 54

<211> 470

<212> PRT

<213> [Clostridium] ultunense Esp

<400> 54

Met Tyr Gln Val Ile Val Lys Asn Ala Arg Ile Pro Gln Gly Asp Glu

1 5 10 15

Thr Val Leu Thr Asn Ile Leu Ile Lys Asp Glu Arg Ile Ala Gly Phe

20 25 30

Val Asp Gly Ile Asp Gly Ile Glu Ala Glu Glu Ile Ile Asp Ala Glu

35 40 45

Gly His Leu Thr Leu Pro Gly Cys Ile Asp Ser His Thr His Phe Met

50 55 60

Asp Pro Gly Phe Thr His Arg Glu Asn Phe Leu Thr Gly Thr Ser Ser

65 70 75 80

Ala Ala Ala Gly Gly Leu Thr Thr Ile Val Asp Met Pro Cys Cys Ser

85 90 95

Val Pro Ser Val Arg Ser Ile Pro Glu Leu Glu Asn Lys Leu Glu Lys

100 105 110

Ile Lys Asp Lys Ala Ile Ile Asp Tyr Gly Phe Trp Gly Gly Val Thr

115 120 125

Gly Glu Asp Val Arg Glu Gly Trp Leu His Asn Val Lys Glu Gln Ala

130 135 140

Asp Tyr Gly Val Ile Gly Phe Lys Val Tyr Met Thr Pro Ser Val Pro

145 150 155 160

Thr Tyr Pro Arg Val Thr Asp Pro Glu Met Tyr Glu Ala Phe Arg Thr

165 170 175

Val Ala Glu Thr Gly Leu Pro Ile Gly Ile His Ala Glu Asn Phe Ala

180 185 190

Met Cys Asp Tyr Phe Val Lys Lys Phe Gln Lys Glu Gly Arg Met Glu

195 200 205

Gly Pro Ala Trp Ala Glu Ala Arg Met Glu Leu Ala Glu Lys Val Ala

210 215 220

Ile Gln Leu Gly Ile Ser Phe Ala Glu Asp Thr Gly Ala Arg Leu His

225 230 235 240

Ile Val His Met Ser Thr Gly Ile Gly Ala Lys Leu Val Lys Glu Ala

245 250 255

Lys Leu Lys Gly Leu Asp Val Thr Ser Glu Thr Cys Pro His Tyr Leu

260 265 270

Thr Leu Asn Tyr Glu Glu Ala Met Ser Glu Tyr Gly Ala Phe Ala Lys

275 280 285

Ile Ala Pro Pro Leu Arg Thr Lys Arg Asp Asn Lys Glu Leu Trp Glu

290 295 300

Gly Leu Arg Asp Gly Ser Val Asp Phe Ile Ala Thr Asp His Ala Pro

305 310 315 320

Tyr His Ile Glu Asp Glu Lys Ala Lys Lys Gly Met Asn Ile Trp Thr

325 330 335

Ala Phe Pro Gly Ile Pro Gly Val Glu Thr Met Val Pro Ile Ile Val

340 345 350

Ser Glu Gly Tyr Asn Lys Gly Arg Ile Ser Leu Ser Lys Met Val Glu

355 360 365

Ile Leu Ser Thr Asn Ala Ala Ile His Tyr Gly Leu Tyr Pro Lys Lys

370 375 380

Gly Ser Leu Asn Ile Gly Ala Asp Ala Asp Leu Thr Ile Ile Asp Leu

385 390 395 400

Asp Lys Glu Trp Ala Ile Asp Gln Glu Lys Thr Tyr Ser Met Ala Lys

405 410 415

Tyr Asn Pro Leu His Gly Leu Lys Leu Lys Gly Lys Val Ala Lys Thr

420 425 430

Val Val Arg Gly Lys Val Val Phe Asp Glu Lys Glu Gly Ile Thr Val

435 440 445

Lys Glu Gly Tyr Gly Gln Phe Val Lys Arg Gln Ser Ile Gln Lys Leu

450 455 460

Asp Lys Lys Ile Lys Phe

465 470

<210> 55

<211> 497

<212> PRT

<213> Bacillus freudenreichii

<400> 55

Met Asp Leu Ile Leu Lys Asn Ala Leu Ile Pro Gln Gly Asp Arg Lys

1 5 10 15

Val Lys Thr Asn Ile Leu Val Asp Asp Gly Lys Ile Val Gly Phe Thr

20 25 30

Ser Ala Leu Asn Gly Leu Ser Ala Asp Arg Val Ile Asp Met Lys Glu

35 40 45

Asn Leu Val Val Pro Gly Cys Ile Asp Pro His Thr His Phe Met Asp

50 55 60

Pro Gly Phe Thr His Arg Glu Thr Phe Thr Thr Gly Thr Arg Ser Ala

65 70 75 80

Ala Ala Gly Gly Val Thr Thr His Ile Asp Met Pro Cys Cys Ser Lys

85 90 95

Pro Ser Val Arg Asp Ala Glu Ser Phe His Lys Lys Leu Ala Pro Leu

100 105 110

Lys Asp Gln Ala Tyr Ile Asp Phe Ala Phe Trp Gly Gly Met Thr Gly

115 120 125

Glu Asp Val Arg Glu Gly Leu Leu Asp Asn Ile Gln Pro Gln Ile Asp

130 135 140

Glu Gly Val Val Ala Phe Lys Val Tyr Met Thr Pro Ser Val Pro Thr

145 150 155 160

Phe Pro Lys Val Ser Asp Ala Glu Met Leu Glu Ile Phe Ser His Ile

165 170 175

Ala Pro Thr Gly Met Pro Ile Gly Val His Ala Glu Asn Tyr Asp Ile

180 185 190

Cys Thr Phe Tyr Ser Glu Lys Leu Lys Lys Glu Gly Arg Leu Asp Gly

195 200 205

Pro Ala Trp Ala Glu Ala Arg Met Ala Leu Ala Glu Lys Ala Ala Ile

210 215 220

Gln Leu Ile Ile Ser Phe Ala Glu Ala Thr Gly Ala Arg Val His Val

225 230 235 240

Val His Met Ser Thr Lys Glu Gly Val Glu Leu Val Arg Ala Ala Lys

245 250 255

Lys Arg Gly Val Lys Val Thr Ala Glu Thr Cys Pro His Tyr Leu Met

260 265 270

Leu Asn Ala Glu Asp Ser Met Ser Glu Arg Gly Thr Phe Ala Lys Ile

275 280 285

Ala Pro Pro Leu Arg Glu Lys Glu Asp Asn Ile Val His Trp Gln Ala

290 295 300

Leu Ala Asp Gly Thr Ile Asp Phe Val Gly Thr Asp His Ala Pro Tyr

305 310 315 320

Glu Ile Pro Thr Glu Lys Asp Ala Pro Asp Ser Thr Ile Trp Asn Ser

325 330 335

Phe Pro Gly Ile Pro Gly Val Glu Thr Met Val Pro Ile Leu Val Ser

340 345 350

Glu Gly Leu Asn Lys Gly Arg Leu Ser Leu Ser Arg Phe Val Glu Val

355 360 365

Thr Ser Arg Asn Ala Ala Ile His Phe Gly Ile Tyr Pro Lys Lys Gly

370 375 380

Ala Met Glu Ile Gly Ser Asp Ala Asp Phe Thr Val Ile Asp Leu Glu

385 390 395 400

Arg Glu Tyr Thr Ile Asp Glu Lys Lys Thr Glu Ser Met Ala Lys Tyr

405 410 415

Asn Pro Leu His Gly Leu Lys Leu Lys Gly Lys Pro Ile Gln Thr Ile

420 425 430

Val Arg Gly Arg Val Val Tyr Asp Glu Asp Asn Gly Gly Ile Met Gly

435 440 445

Glu Ala Gly Tyr Gly Gln Tyr Val Lys Arg Gln Ser Ile Gln Ser Leu

450 455 460

Glu Arg Val Ile Lys Tyr Glu Thr Tyr Glu Glu Gly Ala Lys Glu Ala

465 470 475 480

Glu Ala Lys Pro Arg Met Glu Lys Ala Leu Lys Arg Asp Leu Ile Thr

485 490 495

Asn

<210> 56

<211> 471

<212> PRT

<213> Caloramator fervidus

<400> 56

Met Arg Tyr Asp Cys Ile Val Lys Asn Ala Arg Ile Pro Gln Gly Asp

1 5 10 15

Asp Thr Val Leu Thr Asn Ile Leu Val Lys Asp Glu Lys Ile Ala Gly

20 25 30

Phe Val Asp Asn Ile Glu Gly Ile Glu Ala Asp Thr Ile Ile Asp Ala

35 40 45

Glu Gly His Leu Thr Leu Pro Gly Cys Ile Asp Ser His Thr His Tyr

50 55 60

Met Asp Pro Gly Phe Thr His Arg Glu Asn Phe Leu Thr Gly Thr Lys

65 70 75 80

Gly Ala Ala Val Gly Gly Val Thr Thr Ile Ile Asp Met Pro Cys Cys

85 90 95

Ser Val Pro Ser Val Arg Gly Val Ala Glu Leu Tyr Asn Lys Ile Asn

100 105 110

Ala Ile Lys Asp Lys Ala Ile Val Asp Tyr Ala Met Trp Gly Gly Val

115 120 125

Thr Gly Glu Asp Val Arg Asn Lys Lys Leu Asn Ile Ile Glu Glu Gln

130 135 140

Ala Ala Glu Gly Val Val Ala Phe Lys Ile Tyr Met Thr Pro Ser Val

145 150 155 160

Pro Ser Tyr Pro Arg Val Thr Asp Pro Glu Met Leu Glu Ile Phe Lys

165 170 175

Glu Val Ala Lys Thr Gly Leu Pro Val Gly Val His Ala Glu Asn Phe

180 185 190

Ala Ile Cys Asp Tyr Met Val Lys Lys Phe Gln Glu Glu Gly Arg Met

195 200 205

Asp Gly Pro Ala Trp Ala Glu Ala Arg Met Glu Leu Ala Glu Lys Val

210 215 220

Ala Ile Gln Leu Cys Ile Ser Tyr Ala Glu Glu Thr Gly Ala Arg Phe

225 230 235 240

His Val Val His Met Ser Thr Gly Ile Gly Ala Lys Leu Val Lys Glu

245 250 255

Ala Lys Met Lys Gly Leu Asp Val Thr Ala Glu Thr Cys Pro His Tyr

260 265 270

Leu Thr Leu Asn Tyr Gln Glu His Met Thr Lys Tyr Lys Gln Phe Val

275 280 285

Lys Ile Ala Pro Pro Ile Arg Thr Lys Lys Asp Asn Glu Glu Leu Trp

290 295 300

Lys Gly Leu Gln Asp Gly Ser Val Asp Phe Ile Ala Thr Asp His Ala

305 310 315 320

Pro Tyr Glu Ile Ala Thr Glu Lys Asp Ala Pro Gly Met Asn Ile Trp

325 330 335

Thr Ala Phe Pro Gly Ile Pro Gly Thr Glu Thr Met Val Pro Ile Met

340 345 350

Val Ser Glu Gly Tyr Asn Lys Gly Arg Ile Ser Leu Ser Arg Leu Val

355 360 365

Glu Ile Leu Ser Thr Asn Pro Ala Lys His Tyr Gly Leu Tyr Pro Lys

370 375 380

Lys Gly Ala Leu Met Ile Gly Ser Asp Ala Asp Phe Thr Ile Ile Asp

385 390 395 400

Leu Asn Lys Glu Trp Ile Ile Lys Lys Glu Glu Ile Gln Cys Leu Asn

405 410 415

Lys Tyr Thr Pro Phe Asp Gly Met Lys Leu Lys Gly Lys Val Val Lys

420 425 430

Thr Ile Val Arg Gly Arg Leu Val Tyr Asp Glu Glu Lys Gly Ile Val

435 440 445

Gly Glu Pro Gly Tyr Gly Lys Trp Ile Lys Arg Gln Ser Ile Gln Lys

450 455 460

Leu Asp Arg Val Leu Lys Phe

465 470

<210> 57

<211> 486

<212> PRT

<213> 海泥芽孢杆菌(Bacillus oceanisediminis)

<400> 57

Met Asp Leu Ile Leu Lys Asn Ala Asn Ile Pro Gln Gly Asp Arg Gln

1 5 10 15

Val Leu Thr Asn Ile Leu Val Asn Lys Gly Lys Ile Val Gly Tyr Thr

20 25 30

Asn Asp Ile Ser Phe Leu Asn Ala Ala Arg Val Ile Asp Ile Lys Gly

35 40 45

Lys Leu Val Val Pro Gly Cys Ile Asp Pro His Thr His Phe Met Asp

50 55 60

Pro Gly Phe Thr His Arg Glu Thr Phe Ala Thr Gly Ser Arg Ser Ala

65 70 75 80

Ala Ala Gly Gly Leu Thr Thr Ile Ile Asp Met Pro Cys Cys Ser Lys

85 90 95

Pro Ser Val Arg Asp Gly Glu Ser Phe Asn Lys Lys Ile Gly Pro Ile

100 105 110

Arg Asp Gln Ala Tyr Ile Asp Tyr Cys Phe Trp Gly Gly Met Thr Gly

115 120 125

Glu Asp Ala Arg Glu Gly Trp Ile Asp Asn Ile Tyr Pro Gln Ile Asp

130 135 140

Gln Gly Val Val Ala Phe Lys Val Tyr Met Thr Pro Ser Val Pro Thr

145 150 155 160

Phe Pro Lys Val Ser Asp Ala Glu Met Leu Glu Ile Phe Ser Asn Val

165 170 175

Ala Lys Thr Gly Leu Pro Ile Gly Val His Ala Glu Asn Tyr Asp Ile

180 185 190

Cys Thr Phe Tyr Ser Lys Lys Leu Glu Lys Glu Gly Arg Leu Asp Gly

195 200 205

Pro Ala Trp Ala Glu Ala Arg Ser Ser Leu Ala Glu Lys Val Ala Ile

210 215 220

Glu Leu Ile Leu Ser Phe Ala Glu Ala Thr Asp Ala Arg Val His Val

225 230 235 240

Val His Met Ser Thr Lys Glu Gly Val Glu Leu Val Lys Ala Ala Lys

245 250 255

Lys Arg Gly Val Lys Val Thr Ala Glu Thr Cys Pro His Tyr Leu Val

260 265 270

Leu Asn Ala Lys Asp Ser Met Ser Glu Arg Gly Ser Tyr Ala Lys Ile

275 280 285

Ala Pro Pro Leu Arg Gly Lys Glu Asp Asn Glu Ser His Trp Gln Ala

290 295 300

Leu Ala Asp Gly Thr Ile Asp Phe Val Gly Thr Asp His Ala Pro Tyr

305 310 315 320

Glu Ile Ala Thr Glu Lys Asp Tyr Pro Gly Ala Thr Ile Trp Asn Thr

325 330 335

Phe Pro Gly Ile Pro Gly Val Glu Thr Met Val Pro Ile Leu Val Ser

340 345 350

Glu Gly Leu Asn Lys Gly Arg Leu Ser Leu Ser Arg Phe Val Glu Val

355 360 365

Thr Ser Arg Asn Ala Ala Ile His Tyr Gly Ile Tyr Pro Lys Lys Gly

370 375 380

Ser Met Glu Ile Gly Ser Asp Ala Asp Phe Thr Val Ile Asp Leu Glu

385 390 395 400

Lys Glu Tyr Val Ile Asp Glu Gln Lys Thr Glu Ser Met Ala Lys Tyr

405 410 415

Asn Pro Leu His Gly Met Lys Leu Lys Gly Lys Pro Val Gln Thr Ile

420 425 430

Ile Arg Gly Asn Val Ile Tyr Asp Glu Asp Lys Gly Ile Val Gly Glu

435 440 445

Ala Gly Tyr Gly Glu Tyr Val Pro Arg Gln Ser Ile Gln Arg Leu Glu

450 455 460

Arg Thr Leu Lys Tyr Glu Lys Tyr Glu Ala Val Lys Ala Lys Thr Lys

465 470 475 480

Asp Pro Val Ala Gln Gln

485

<210> 58

<211> 533

<212> PRT

<213> 非解糖卟啉单胞菌(Soehngenia saccharolytica)

<400> 58

Met Met Tyr Asp Val Ile Ile Lys Asn Ala Arg Ile Pro Gln Ala Asp

1 5 10 15

Glu Thr Thr Leu Thr Asn Ile Leu Val Lys Asp Glu Lys Ile Ala Gly

20 25 30

Phe Ile Asp Asn Ile Asp Gly Ile Asp Ala Lys Glu Ile Ile Asp Ala

35 40 45

Asn Gly His Leu Thr Leu Pro Gly Cys Ile Asp Ser His Thr His Phe

50 55 60

Met Tyr Gln Gly Phe Pro His Arg Glu Asn Phe Leu Thr Gly Thr Ala

65 70 75 80

Ala Ala Ala Ala Gly Gly Val Thr Thr Ile Ile Asp Met Pro Cys Cys

85 90 95

Ser Val Pro Ser Val Arg Ser Val Glu Gln Leu Lys Leu Lys Leu Asp

100 105 110

Ala Val Ser Pro Gln Ala Leu Val Asp Phe Ala Phe Trp Gly Gly Val

115 120 125

Thr Gly Glu Asp Val Arg Asn Gly Trp Leu His Asn Val Glu Glu Gln

130 135 140

Ala Glu Tyr Gly Val Val Ala Tyr Lys Ala Tyr Met Thr Pro Ser Val

145 150 155 160

Pro Thr Tyr Pro Arg Val Asp Asp Pro Glu Met Leu Glu Ala Phe Thr

165 170 175

Glu Val Ala Lys Thr Gly Leu Pro Ile Gly Ile His Ala Glu Asn Tyr

180 185 190

Ala Met Cys Asp Tyr Tyr Val Lys Lys Tyr Gln Arg Glu Gly Arg Thr

195 200 205

Asp Gly Pro Ala Trp Ala Glu Ala Arg Leu Glu Leu Ala Glu Lys Val

210 215 220

Ala Ile Glu Leu Gly Ile Ser Phe Ala Glu His Thr Gly Ala Arg Leu

225 230 235 240

His Ile Val His Met Ser Thr Gly Ile Gly Ala Lys Leu Ile Gly Glu

245 250 255

Gly Lys Lys Arg Gly Leu Asn Val Thr Ser Glu Thr Cys Pro His Tyr

260 265 270

Leu Thr Leu Asn Tyr Gln Glu Ala Met Thr Lys Tyr Gly Pro Leu Ala

275 280 285

Lys Ile Ala Pro Pro Leu Arg Thr Lys Lys Asp Asn Glu Glu Leu Trp

290 295 300

Glu Gly Leu Arg Asn Gly Ser Val Asp Phe Met Ala Thr Asp His Ala

305 310 315 320

Pro Tyr Glu Leu Glu Ser Glu Lys Leu Ser Lys Gly Met Asn Ile Trp

325 330 335

Thr Ser Phe Pro Gly Ile Pro Gly Val Glu Thr Met Val Pro Val Leu

340 345 350

Ile Ser Glu Gly Tyr Asn Lys Gly Arg Leu Ser Leu Ser Lys Leu Val

355 360 365

Glu Val Leu Ser Thr Asn Ala Ala Lys His Tyr Gly Ile Tyr Pro Lys

370 375 380

Lys Gly Ala Met Asn Ile Gly Ser Asp Ala Asp Phe Thr Ile Ile Asp

385 390 395 400

Leu Asp Lys Arg Trp Lys Val Asp Pro Lys Lys Leu Ala Ser Met Cys

405 410 415

Gly Tyr Thr Pro Leu Glu Gly Met Glu Leu Lys Gly Lys Val Ala Lys

420 425 430

Thr Val Leu Arg Gly His Leu Ile Tyr Glu Asp Asn Asp Glu Ser Glu

435 440 445

Leu Ala Lys Met Thr Asp Glu Asp Met Gln Arg Ile Val His Asn Tyr

450 455 460

Pro Glu Gly Leu Glu Glu Arg Tyr Lys Asp Ile Leu Lys Glu Phe Pro

465 470 475 480

His Leu His Ser Lys Glu Tyr Glu Asn Ser Tyr Arg Lys Gln His Pro

485 490 495

Glu Ile Ile Asp Glu His Ile Arg Gly Ile Lys Gly Ile Lys Val Lys

500 505 510

Pro Gly Phe Gly Gln Phe Val Lys Arg Gln Ser Ile Gln Val Leu Pro

515 520 525

Arg Arg Ile Thr Phe

530

<210> 59

<211> 486

<212> PRT

<213> 坚强芽孢杆菌(Bacillus firmus )

<400> 59

Met Asp Leu Ile Leu Lys Asn Ala Asn Ile Pro Gln Gly Asp Arg Gln

1 5 10 15

Val Leu Thr Asn Ile Leu Val Asn Glu Gly Lys Ile Val Gly Tyr Thr

20 25 30

Asn Asp Ile Ser Phe Leu Asn Ala Ala Lys Val Ile Asp Ile Gln Gly

35 40 45

Lys Leu Val Val Pro Gly Cys Ile Asp Pro His Thr His Phe Met Asp

50 55 60

Pro Gly Phe Thr His Arg Glu Thr Phe Ala Thr Gly Ser Arg Ser Ala

65 70 75 80

Ala Ala Gly Gly Leu Thr Thr Ile Ile Asp Met Pro Cys Cys Ser Lys

85 90 95

Pro Ser Val Arg Asp Gly Asp Ser Phe Asn Lys Lys Ile Gly Pro Ile

100 105 110

Arg Asp Gln Ala Tyr Ile Asp Tyr Cys Phe Trp Gly Gly Met Thr Gly

115 120 125

Glu Asp Ala Arg Glu Gly Trp Ile Asp Asn Ile Tyr Pro Gln Ile Asp

130 135 140

Gln Gly Val Val Ala Phe Lys Val Tyr Met Thr Pro Ser Val Pro Thr

145 150 155 160

Phe Pro Lys Val Ser Asp Ala Glu Met Leu Glu Ile Phe Lys Asn Val

165 170 175

Ala Lys Thr Gly Leu Pro Ile Gly Val His Ala Glu Asn Tyr Asp Ile

180 185 190

Cys Thr Phe Asn Ser Lys Lys Leu Glu Lys Glu Gly Arg Leu Asp Gly

195 200 205

Pro Ala Trp Ala Glu Ala Arg Ser Ser Leu Ala Glu Lys Val Ala Ile

210 215 220

Glu Leu Ile Leu Ser Phe Ala Glu Ala Thr Asp Ala Arg Val His Val

225 230 235 240

Val His Met Ser Thr Lys Glu Gly Val Gln Leu Val Lys Ala Ala Lys

245 250 255

Lys Arg Gly Val Lys Val Thr Ala Glu Thr Cys Pro His Tyr Leu Val

260 265 270

Leu Asn Ala Gln Asp Ser Met Ser Glu Arg Gly Ser Tyr Ala Lys Ile

275 280 285

Ala Pro Pro Leu Arg Gly Lys Glu Asp Asn Glu Ser His Trp Gln Ala

290 295 300

Leu Ala Asp Gly Thr Ile Asp Phe Val Gly Thr Asp His Ala Pro Tyr

305 310 315 320

Glu Ile Ala Thr Glu Lys Asp Tyr Pro Gly Ala Thr Ile Trp Asn Thr

325 330 335

Phe Pro Gly Ile Pro Gly Val Glu Thr Met Val Pro Ile Leu Val Ser

340 345 350

Glu Gly Leu Asn Lys Gly Arg Ile Ser Leu Ser Arg Phe Val Glu Val

355 360 365

Thr Ser Arg Asn Ala Ala Ile His Tyr Gly Ile Tyr Pro Lys Lys Gly

370 375 380

Ser Met Glu Ile Gly Ser Asp Ala Asp Phe Thr Val Ile Asp Leu Glu

385 390 395 400

Lys Glu Tyr Val Ile Asp Glu Gln Lys Thr Glu Ser Met Ala Lys Tyr

405 410 415

Asn Pro Leu His Gly Met Lys Leu Lys Gly Lys Pro Val Gln Thr Ile

420 425 430

Ile Arg Gly Asn Val Val Phe Glu Glu Asp Lys Gly Ile Val Gly Glu

435 440 445

Ala Gly Tyr Gly Glu Tyr Val Pro Arg Gln Ser Ile Gln Arg Leu Glu

450 455 460

Arg Thr Leu Lys Tyr Glu Lys Tyr Glu Ala Val Asn Val Lys Thr Lys

465 470 475 480

Asp Pro Val Ala Gln Gln

485

<210> 60

<211> 471

<212> PRT

<213> Thermotalea metallivorans

<400> 60

Met Gln Val Asp Val Met Ile Lys Asn Ala Arg Ile Pro Gln Gly Asn

1 5 10 15

Asp Thr Ile Leu Thr Asn Ile Leu Ile Val Asp Glu Lys Ile Thr Gly

20 25 30

Phe Val Pro Asn Thr Glu Gly Ile Glu Ala Ala Ser Val Ile Asp Ala

35 40 45

Glu Gly His Leu Thr Ile Pro Gly Cys Ile Asp Ser His Thr His Ile

50 55 60

Asn Asp Pro Gly Phe Thr His Arg Glu Asn Phe Leu Thr Gly Thr Ser

65 70 75 80

Ala Ala Ala Ser Gly Gly Val Thr Thr Ile Ile Asp Met Pro Cys Cys

85 90 95

Ser Val Pro Ser Val Arg Ser Ile Glu Asn Leu Glu His Lys Leu Asn

100 105 110

Ala Ile Lys Asp Lys Ala Ile Ile Asp Tyr Ala Met Trp Gly Gly Val

115 120 125

Thr Gly Glu Asp Val Arg Glu Gly Trp Leu His His Val Lys Glu Gln

130 135 140

Ala Asp Tyr Gly Val Val Ala Phe Lys Val Tyr Met Thr Pro Ser Val

145 150 155 160

Pro Thr Tyr Pro Arg Val Thr Asp Pro Glu Met Leu Glu Ala Phe Arg

165 170 175

Ala Val Ala Glu Thr Gly Leu Pro Ile Gly Ile His Ala Glu Asn Phe

180 185 190

Ala Met Cys Asp Tyr Tyr Val Lys Lys Phe Gln Asn Glu Gly Arg Met

195 200 205

Asp Gly Pro Ala Trp Ala Glu Ala Arg Ser His Leu Ala Glu Lys Val

210 215 220

Ala Ile Glu Leu Gly Ile Ser Phe Ala Glu Glu Thr Gly Ala Arg Leu

225 230 235 240

His Ile Val His Met Ser Thr Gly Ile Gly Ala Lys Leu Val Gly Glu

245 250 255

Ala Lys Lys Arg Gly Val Asp Val Thr Ala Glu Thr Cys Pro His Tyr

260 265 270

Leu Thr Leu Asn Tyr Lys Asp Ala Met Thr Glu Phe Lys Gln Phe Ala

275 280 285

Lys Ile Ala Pro Pro Leu Arg Cys Lys Glu Asp Asn Glu Ile His Trp

290 295 300

Glu Ala Leu Arg Asp Gly Arg Ile Asp Phe Val Ala Thr Asp His Ala

305 310 315 320

Pro Tyr Glu Ile Pro Thr Glu Lys Glu Ala Glu Gly Met Asn Ile Trp

325 330 335

Thr Ala Phe Pro Gly Ile Pro Gly Val Glu Thr Met Val Pro Val Ile

340 345 350

Val Ser Glu Gly Tyr Asn Lys Gly Arg Leu Ser Leu Ser Arg Met Val

355 360 365

Glu Val Leu Ser Thr Ala Pro Ala Lys His Tyr Gly Ile Tyr Pro Lys

370 375 380

Lys Gly Ala Met His Ile Gly Ser Asp Ala Asp Phe Thr Ile Ile Asp

385 390 395 400

Leu Glu Lys Glu Trp Val Ile Asp Lys Asp Lys Met Tyr Thr Met Ala

405 410 415

Lys Tyr Thr Pro Phe His Gly Phe Lys Leu Lys Gly Lys Pro Val Lys

420 425 430

Thr Val Val Arg Gly Lys Leu Val Tyr Asp Asp Lys Val Gly Ile Val

435 440 445

Gly Arg Glu Gly His Gly Gln Phe Ile Arg Arg Gln Ser Ile Gln Lys

450 455 460

Leu Asp Arg Ile Leu Lys Phe

465 470

<210> 61

<211> 493

<212> PRT

<213> Ornithinibacillus halophilus

<400> 61

Met Asp Leu Ile Leu Lys Asn Ala Leu Ile Pro Gln Gly Asp Arg Lys

1 5 10 15

Val Gln Thr Asn Ile Leu Val Asp Asn Gly Lys Ile Thr Gly Tyr Thr

20 25 30

Asn Ser Val Asp Gly Leu Tyr Ala Asp Glu Ile Leu Asp Met Lys Gly

35 40 45

Asn Leu Val Val Pro Gly Cys Ile Asp Pro His Thr His Phe Met Asp

50 55 60

Pro Gly Phe Thr His Arg Glu Thr Phe Thr Thr Gly Ser Arg Ser Ala

65 70 75 80

Ala Ala Gly Gly Val Thr Thr His Ile Asp Met Pro Cys Cys Ser Lys

85 90 95

Pro Ser Val Arg Asp Gly Asp Ser Phe His Lys Lys Ile Thr Pro Leu

100 105 110

Lys Gly Gln Ala Tyr Ile Asp Tyr Cys Phe Trp Gly Gly Met Thr Gly

115 120 125

Glu Asp Val Arg Asn Gly Trp Leu Asn Asn Ile Tyr Pro Gln Ile Glu

130 135 140

Glu Gly Val Val Ala Phe Lys Val Tyr Met Thr Pro Ser Val Pro Thr

145 150 155 160

Phe Pro Lys Val Thr Asp Ala Glu Met Leu Glu Ile Phe Ser His Ile

165 170 175

Ala Pro Thr Gly Met Pro Ile Gly Val His Ala Glu Asn Tyr Asp Ile

180 185 190

Cys Thr Phe Tyr Ser Glu Lys Leu Lys Lys Glu Gly Arg Met Asp Gly

195 200 205

Pro Ala Trp Ala Glu Ala Arg Met Pro Leu Ala Glu Lys Val Ala Ile

210 215 220

Gln Leu Ile Leu Ser Tyr Ala Glu Ala Thr Asp Ala Arg Val His Val

225 230 235 240

Val His Met Ser Thr Lys Glu Gly Val Glu Leu Val Arg Asp Ala Lys

245 250 255

Lys Arg Gly Val Lys Val Thr Ala Glu Thr Cys Pro His Tyr Leu Met

260 265 270

Leu Asn Ala Gln Asp Ser Met Thr Glu Arg Gly Thr Phe Ala Lys Ile

275 280 285

Ala Pro Pro Leu Arg Glu Arg Met Asp Asn Ile Val His Trp Gln Ala

290 295 300

Leu Ala Asp Gly Thr Ile Asp Phe Val Gly Thr Asp His Ala Pro Tyr

305 310 315 320

Glu Ile Glu Thr Glu Lys Asp Ala Pro Asp Ser Asp Ile Trp Asn Ser

325 330 335

Phe Pro Gly Ile Pro Gly Val Glu Thr Met Val Pro Ile Leu Val Ser

340 345 350

Glu Gly Leu Asn Lys Gly Arg Leu Ser Leu Ser Arg Phe Val Asp Val

355 360 365

Thr Ser Arg Asn Ala Ala Ile His Tyr Gly Ile Tyr Pro Lys Lys Gly

370 375 380

Ser Met Glu Leu Gly Ala Asp Ala Asp Phe Thr Val Ile Asp Leu Asp

385 390 395 400

Arg Glu Tyr Met Ile Asp Glu Lys Gln Thr Glu Ser Met Ala Lys Tyr

405 410 415

Asn Pro Leu His Gly Leu His Leu Lys Gly Lys Pro Ile Gln Thr Ile

420 425 430

Val Arg Gly Lys Val Val Tyr Asp Glu Asp Asn Gly Gly Ile Val Gly

435 440 445

Glu Ala Gly Phe Gly Glu Tyr Val Lys Arg Gln Ser Ile Gln Arg Leu

450 455 460

Asp Arg Glu Ile Lys Tyr Glu Val Tyr Glu Glu Lys Ala Lys Glu Leu

465 470 475 480

Glu Ser Thr Lys Arg Ile Glu His Ala Leu Val Gln Lys

485 490

<210> 62

<211> 475

<212> PRT

<213> 解脂嗜热互营杆菌(Thermosyntropha lipolytica)

<400> 62

Met Lys Phe Asp Thr Val Val Lys Asn Ala Met Ile Pro Gln Gly Asp

1 5 10 15

Arg Leu Val Pro Ala Thr Ile Leu Val Lys Asp Gly Val Ile Ala Gly

20 25 30

Ile Val Asn Gly Glu Met Glu Pro His Leu Leu Glu Gly Val Asn Val

35 40 45

Ile Asp Ala Glu Gly Asn Met Val Leu Pro Gly Cys Ile Asp Ser His

50 55 60

Thr His Phe Met Asp Pro Gly Phe Thr His Arg Glu Thr Phe Leu Asp

65 70 75 80

Gly Cys Lys Ala Ala Ala Ala Gly Gly Leu Thr Thr Ile Ile Asp Met

85 90 95

Pro Cys Cys Ser Val Pro Ser Val Arg Gly Val Ala Glu Met Glu Asn

100 105 110

Lys Leu Asn Ala Ile Lys Pro Gln Ala Ile Ile Asp Phe Gly Leu Trp

115 120 125

Gly Gly Val Thr Gly Glu Asp Val Arg Asn Glu Val His Tyr Lys Ile

130 135 140

Val Arg Glu Gln Ala Asp Ala Gly Val Val Ala Phe Lys Val Tyr Met

145 150 155 160

Thr Pro Ser Val Pro Ser Tyr Pro Arg Val Thr Asp Pro Glu Met Leu

165 170 175

Glu Cys Phe Lys Ala Val Ala Glu Thr Gly Leu Pro Ile Gly Ile His

180 185 190

Ala Glu Asn Tyr Ala Met Cys Asp Phe Tyr Val Gln Lys Phe Gln Lys

195 200 205

Glu Gly Arg Met Asp Gly Pro Ala Trp Ser Glu Ala Arg Met Pro Leu

210 215 220

Ala Glu Lys Val Ala Ile Gln Leu Gly Ile Ser Phe Ala Glu Ala Thr

225 230 235 240

Gly Ala Arg Leu His Ile Val His Met Ala Leu Gly Glu Gly Ala Ile

245 250 255

Leu Val Gly Glu Ala Lys Lys Arg Gly Leu Asp Val Thr Ser Glu Thr

260 265 270

Cys Pro His Tyr Leu Val Leu Asp Tyr Lys Glu Ala Met Ser Lys Tyr

275 280 285

Gly Ala Phe Ala Lys Ile Ala Pro Pro Leu Arg Ser Arg Glu Asp Asn

290 295 300

Glu Leu Leu Trp Gln Gly Leu Gln Asn Gly Ser Val Asp Phe Ile Ala

305 310 315 320

Thr Asp His Ala Pro Tyr Glu Ile Pro Thr Glu Lys Ala Ala Glu Asp

325 330 335

Met Thr Ile Trp Thr Ala Phe Pro Gly Ile Pro Gly Val Glu Thr Met

340 345 350

Val Pro Ile Ile Ile Ser Glu Gly Tyr Asn Lys Gly Arg Ile Lys Leu

355 360 365

Ser Arg Leu Val Glu Ile Leu Ser Arg Asn Ala Ala Ile His Tyr Gly

370 375 380

Leu Tyr Pro Lys Lys Gly Ala Met Glu Ile Gly Ser Asp Ala Asp Phe

385 390 395 400

Thr Ile Ile Asp Leu Asn Lys Glu Trp Val Ile Asp Gln Glu Lys Gln

405 410 415

Tyr Ser Lys Ala Lys Tyr Thr Pro Leu His Gly Met Lys Leu Lys Gly

420 425 430

Lys Val Val Lys Thr Val Val Arg Gly Lys Val Val Tyr Asp Glu Ala

435 440 445

Lys Gly Val Ile Gly Glu Pro Gly Tyr Gly Glu Phe Val Lys Arg Gln

450 455 460

Thr Ile Ser Lys Leu Asp Arg Lys Ile Thr Phe

465 470 475

<210> 63

<211> 490

<212> PRT

<213> 虚构芽孢杆菌(Fictibacillus sp.)

<400> 63

Met Asp Leu Ile Leu Lys Asn Ala Met Ile Pro Gln Gly Asp Arg Thr

1 5 10 15

Val Ile Thr Asn Ile Leu Val Gln Glu Gly Arg Ile Thr Gly Phe Cys

20 25 30

Asn Asp Ile Gln Thr Leu Asn Ala Asp Lys Val Leu Asp Met Glu Gly

35 40 45

Lys Leu Val Val Pro Gly Cys Ile Asp Pro His Thr His Phe Met Asp

50 55 60

Pro Gly Phe Thr His Arg Glu Thr Phe Ala Thr Gly Ser Arg Ser Ala

65 70 75 80

Ala Ala Gly Gly Leu Thr Thr Ile Ile Asp Met Pro Cys Cys Ser Lys

85 90 95

Pro Ser Val Arg Asp Gly Glu Ser Phe Gln Lys Lys Ile Ala Pro Ile

100 105 110

Arg Gly Gln Ala Tyr Ile Asp Tyr Cys Phe Trp Gly Gly Met Thr Gly

115 120 125

Glu Asp Ala Arg Asn Gly Trp Ile Asp Asn Ile Tyr Pro Gln Ile Asp

130 135 140

Glu Gly Val Val Ala Phe Lys Val Tyr Met Thr Pro Ser Val Pro Thr

145 150 155 160

Phe Pro Lys Val Ser Asp Gly Glu Met Leu Glu Ile Phe Gln Lys Val

165 170 175

Ala Lys Thr Gly Met Pro Ile Gly Val His Ala Glu Asn Tyr Asp Ile

180 185 190

Cys Thr Phe Tyr Thr Glu Lys Leu Lys Asn Glu Gly Arg Glu Asp Gly

195 200 205

Pro Ala Trp Ala Glu Ala Arg Met Ala Leu Ala Glu Lys Val Ala Ile

210 215 220

Glu Leu Ile Ile Ser Tyr Ala Glu Ala Thr Asp Ala Arg Val His Val

225 230 235 240

Val His Met Ser Thr Lys Glu Gly Val Lys Leu Val Arg Glu Ala Lys

245 250 255

Lys Arg Gly Val Lys Val Thr Ser Glu Thr Cys Pro His Tyr Leu Met

260 265 270

Leu Asn Ala Gln Asp Ser Met Thr Glu Arg Gly Thr Phe Ala Lys Ile

275 280 285

Ala Pro Pro Leu Arg Gly Lys Glu Asp Asn Gln Ala His Trp Gln Ala

290 295 300

Leu Ala Asp Gly Thr Ile Asp Phe Val Gly Thr Asp His Ala Pro Tyr

305 310 315 320

Glu Ile Ala Thr Glu Lys Ala Ala Ala Asp Ser Ser Ile Trp Asn Ser

325 330 335

Phe Pro Gly Ile Pro Gly Val Glu Thr Met Val Pro Ile Leu Val Ser

340 345 350

Glu Gly Leu Asn Lys Gly Arg Leu Ser Leu Ser Arg Phe Val Glu Val

355 360 365

Thr Ser Lys Asn Ala Ala Val His Tyr Gly Ile Tyr Pro Lys Lys Gly

370 375 380

Ala Met Glu Ile Gly Ser Asp Ala Asp Phe Thr Val Ile Asp Leu Asp

385 390 395 400

His Glu Trp Val Ile Asp Glu Lys Gln Thr Glu Ser Met Ala Lys Tyr

405 410 415

Asn Pro Leu His Gly Ile Lys Leu Lys Gly Arg Pro Val Gln Thr Ile

420 425 430

Leu Arg Gly Thr Val Val Tyr Asp Tyr Glu Lys Gly Gly Ile Thr Gly

435 440 445

Thr Ala Gly Tyr Gly Glu Tyr Val Lys Arg Gln Ser Ile Gln Arg Leu

450 455 460

Glu Arg Met Leu Lys Tyr Glu Asn Tyr Glu Thr Ala Asn Gln Pro Ser

465 470 475 480

Ala Ala Arg Gln Gln Leu Glu Lys Ser Lys

485 490

<210> 64

<211> 489

<212> PRT

<213> Bacillus mesonae

<400> 64

Met Asp Leu Ile Leu Lys Asn Ala Ser Ile Pro Gln Gly Asp Arg Gln

1 5 10 15

Val Leu Thr Asn Ile Leu Val Asp Glu Gly Lys Ile Val Gly Phe Thr

20 25 30

Asn Asp Ile Asn Phe Leu His Ala Asp Lys Met Ile Asp Ile Glu Gly

35 40 45

Lys Leu Val Val Pro Gly Cys Ile Asp Pro His Thr His Phe Met Asp

50 55 60

Pro Gly Phe Thr His Arg Glu Thr Phe Glu Thr Gly His Arg Ser Ala

65 70 75 80

Ala Ala Gly Gly Leu Thr Leu His Ile Asp Met Pro Cys Cys Ser Val

85 90 95

Pro Ser Val Arg Asp Gly Glu Ser Phe Phe Lys Lys Leu Asn Pro Ile

100 105 110

Lys Glu Lys Ala Tyr Ile Asp Tyr Cys Phe Trp Gly Gly Met Thr Gly

115 120 125

Glu Asp Ala Arg Glu Gly Trp Leu Asp Asn Ile Tyr Pro Gln Ile Glu

130 135 140

Gln Gly Val Val Ala Phe Lys Val Tyr Met Thr Pro Ser Val Pro Thr

145 150 155 160

Phe Pro Lys Val Ser Asp Gly Glu Met Phe Glu Ile Phe Arg Lys Ile

165 170 175

Ala Pro Thr Gly Leu Pro Ile Gly Val His Ala Glu Asn Tyr Asp Ile

180 185 190

Cys Thr Phe Asn Thr Glu Arg Leu Lys Lys Glu Gly Arg Gln Asp Gly

195 200 205

Pro Ala Trp Ala Glu Ala Arg Ser Ser Leu Ala Glu Lys Val Ala Ile

210 215 220

Glu Leu Ile Ile Ser Tyr Ala Glu Ala Val Asp Ala Arg Val His Val

225 230 235 240

Val His Met Ser Thr Lys Glu Gly Val Glu Leu Val Arg Arg Ala Lys

245 250 255

Glu Arg Gly Ile Lys Val Thr Ala Glu Thr Cys Pro His Tyr Leu Met

260 265 270

Leu Asn Ala Lys Asp Ser Met Thr Glu Arg Gly Thr Phe Ala Lys Ile

275 280 285

Ala Pro Pro Leu Arg Gly Lys Glu Asp Asn Glu Ala His Trp Gln Ala

290 295 300

Leu Ala Asp Gly Thr Ile Asp Phe Val Gly Thr Asp His Ala Pro Tyr

305 310 315 320

Glu Ile Ala Thr Glu Lys Asp Ala Pro Asp Ser Asp Ile Trp Asn Ser

325 330 335

Phe Pro Gly Ile Pro Gly Val Glu Thr Met Val Pro Ile Leu Val Ser

340 345 350

Glu Gly Leu Asn Lys Gly Arg Leu Ser Leu Ser Arg Phe Val Glu Val

355 360 365

Thr Ser Thr Asn Ala Ala Lys His Tyr Gly Ile Tyr Pro Lys Lys Gly

370 375 380

Ala Leu Glu Ile Gly Ser Asp Ala Asp Phe Thr Val Ile Asp Leu Asn

385 390 395 400

Arg Glu Tyr Glu Ile Asp Glu Gln Gln Thr Glu Ser Met Ala Lys Tyr

405 410 415

Asn Pro Leu His Gly Leu Lys Leu Lys Gly Lys Pro Val Gln Thr Ile

420 425 430

Val Arg Gly Asn Leu Ile Tyr Asp Glu Asp His Gly Gly Ile Val Gly

435 440 445

Glu Ala Gly Tyr Gly Glu Tyr Val Pro Arg Gln Ser Ile Gln Arg Leu

450 455 460

Glu Arg Val Leu Lys Tyr Glu Lys Tyr Glu Ala Ser Val Val Thr Lys

465 470 475 480

Lys Ser Glu Glu Ala Glu Ile Ala Lys

485

<210> 65

<211> 531

<212> PRT

<213> Clostridium purinilytica

<400> 65

Met Tyr Asp Leu Ile Leu Lys Asn Ala Arg Ile Pro Leu Gly Asp Asn

1 5 10 15

Thr Ile Val Thr Asn Ile Leu Val Lys Asp Glu Lys Ile Ser Gly Ile

20 25 30

Ser Asn Cys Ile Glu Gly Val Glu Ala Lys Glu Thr Ile Asp Val Glu

35 40 45

Glu Gln Leu Thr Leu Pro Gly Cys Ile Asp Ser His Thr His Phe Met

50 55 60

Tyr Gln Gly Phe Pro His Arg Glu Asn Phe Leu Thr Gly Thr Ala Ala

65 70 75 80

Ala Ala Thr Gly Gly Ile Thr Thr Ile Ile Asp Met Pro Cys Cys Thr

85 90 95

Val Pro Ser Ala Arg Ser Val Asp Gln Leu Lys Leu Lys Ile Asp Leu

100 105 110

Cys Gln Pro Gln Ser Val Val Asp Phe Ala Met Trp Gly Gly Val Thr

115 120 125

Gly Glu Asp Val Arg Glu Gly Trp Met His Asn Val Lys Glu Gln Ala

130 135 140

Asp Tyr Gly Val Val Ala Phe Lys Val Tyr Met Thr Pro Ser Val Pro

145 150 155 160

Thr Tyr Pro Arg Val Thr Asp Pro Glu Met Leu Glu Cys Phe Arg Ala

165 170 175

Val Ala Glu Thr Gly Leu Pro Ile Gly Val His Ala Glu Asn Phe Ala

180 185 190

Met Cys Asp Phe Tyr Val Lys Lys Phe Gln Lys Gly Gly Lys Leu Asp

195 200 205

Ala Thr Ala Trp Ser Glu Ala Arg Met Glu Leu Ala Glu Lys Val Ala

210 215 220

Ile Glu Leu Gly Ile Ser Phe Ala Glu Glu Thr Asn Ala Arg Leu His

225 230 235 240

Ile Val His Met Ser Thr Gly Ile Gly Ala Lys Leu Ile Gly Glu Ala

245 250 255

Lys Lys Arg Gly Ile Asn Ala Thr Ser Glu Thr Cys Pro His Tyr Leu

260 265 270

Thr Leu Asn Tyr Gln Asp Ala Leu Lys Glu His Gly Ala Phe Ala Lys

275 280 285

Val Ala Pro Pro Leu Arg Asn Lys Lys Asp Asn Glu Glu Leu Trp Glu

290 295 300

Gly Leu Lys Asn Gly Ser Val Asp Phe Ile Ala Thr Asp His Ala Pro

305 310 315 320

Tyr Glu Ile Glu Ser Glu Lys Asn Arg Glu Asp Met Thr Ile Trp Thr

325 330 335

Ala Phe Pro Gly Phe Pro Gly Val Glu Thr Met Val Pro Ile Leu Ile

340 345 350

Ser Glu Gly Tyr Asn Lys Gly Arg Val Ser Leu Ser Lys Leu Val Asp

355 360 365

Ile Leu Ser Thr Asn Ala Ala Lys His Tyr Gly Leu Tyr Pro Lys Lys

370 375 380

Gly Thr Ile Ala Ile Gly Ser Asp Ala Asp Phe Thr Ile Ile Asp Leu

385 390 395 400

Asn Lys Glu Trp Thr Ile Thr Lys Glu Thr Val Thr Met Cys Gly Tyr

405 410 415

Thr Pro Phe Lys Gly Met Lys Leu Lys Gly Lys Pro Val Lys Thr Ile

420 425 430

Val Arg Gly His Val Val Tyr Asp Glu Lys Gln Glu Gly Thr Leu Pro

435 440 445

Gln Leu Thr Asp Glu Glu Leu Lys Thr Ile Val His Glu Tyr Pro Ser

450 455 460

Gly Val Glu Glu Lys Tyr Ser Glu Ile Phe Glu Lys Phe Pro His Leu

465 470 475 480

His Ser Asp Glu Tyr Lys Arg Ser Tyr Arg Lys Asp His Pro Glu Leu

485 490 495

Ile Asp Lys His Ile Arg Gly Ile Lys Gly Ile Met Val Lys Pro Gly

500 505 510

Phe Gly Gln Phe Val Lys Arg Gln Ser Ile Gln Ile Leu Pro Lys Thr

515 520 525

Ile Thr Tyr

530

<210> 66

<211> 482

<212> PRT

<213> Tindallia magadiensis

<400> 66

Met Lys Val Asp Val Val Val Thr Asn Ala Arg Ile Pro Gln Gly Asp

1 5 10 15

Asp Thr Val Leu Thr Asn Ile Leu Val Lys Asp Glu Lys Ile Ala Gly

20 25 30

Phe Val Ser Glu Leu Glu Gly Val Thr Tyr Asp Arg Glu Ile Asp Ala

35 40 45

Lys Gly His Leu Thr Leu Pro Gly Cys Phe Asp Ser His Thr His Phe

50 55 60

Met Asp Pro Gly Phe Glu His Arg Glu Asn Phe Leu Thr Gly Thr Ser

65 70 75 80

Ser Ala Ala Ala Gly Gly Leu Thr Thr Ile Met Asp Met Pro Cys Cys

85 90 95

Ser Lys Pro Ser Val Arg Ser Ile Pro Glu Leu Glu Ser Lys Leu Asn

100 105 110

Arg Ile Lys Asp Lys Ala Ile Ile Asp Phe Ala Phe Trp Gly Gly Val

115 120 125

Thr Gly Glu Asp Ile Arg Asn Gly Asp Tyr His Val Val Gln Glu Gln

130 135 140

Ala Asp Tyr Gly Val Cys Ala Phe Lys Val Tyr Met Thr Pro Ser Val

145 150 155 160

Pro Thr Tyr Glu Arg Val Thr Asp Pro Glu Met Leu Glu Ala Phe Arg

165 170 175

Leu Val Ala Lys Thr Gly Leu Val Val Gly Ile His Ala Glu Asn Phe

180 185 190

Ala Met Cys Asp Phe Tyr Val Lys Lys Phe Gln Ala Glu Gly Arg Thr

195 200 205

Asp Gly Pro Ala Trp Ala Glu Ala Arg Leu Glu Leu Ala Glu Lys Ala

210 215 220

Ala Ile Gln Leu Gly Ile Ser Phe Ala Glu Glu Thr Gly Ala Arg Leu

225 230 235 240

His Ile Val His Met Ser Thr Gly Ile Gly Ala Lys Leu Val Gly Ala

245 250 255

Ala Lys Gln Lys Gly Leu Asp Val Thr Ser Glu Ala Cys Pro His Tyr

260 265 270

Leu Met Leu Asn Tyr Gln Asp Ser Met Thr Glu His Arg Gln Phe Ala

275 280 285

Lys Ile Ala Pro Pro Leu Arg Thr Lys Lys Asp Asn Glu Glu Leu Trp

290 295 300

Glu Gly Leu Gln Asn Gly Ser Val Asp Phe Met Gly Thr Asp His Ala

305 310 315 320

Pro Tyr Glu Ile Glu Thr Glu Lys Glu Gly Val Val Asp Gly Val Ala

325 330 335

Leu Asp Ile Trp Asn Ser Phe Pro Gly Ile Pro Gly Val Glu Thr Leu

340 345 350

Val Pro Ile Met Val Ser Glu Gly Tyr Leu Lys Gly Arg Leu Ser Leu

355 360 365

Ser Arg Leu Val Glu Val Leu Ser Thr Asn Ala Ala Lys Gln Tyr Gly

370 375 380

Ile Tyr Pro Lys Lys Gly Ala Leu His Ile Gly Ser Asp Ala Asp Phe

385 390 395 400

Thr Ile Ile Asp Leu Glu Lys Glu Trp Thr Ile Asp Gln Lys Glu Thr

405 410 415

Tyr Ser Met Ala Lys Tyr Asn Pro Leu His Gly Ile Lys Leu Lys Gly

420 425 430

Lys Pro Tyr Lys Thr Val Val Arg Gly Asn Thr Val Tyr Glu Asp Gly

435 440 445

Lys Gly Ile Val Gly Lys Pro Gly Tyr Gly Gln Phe Val Lys Arg Gln

450 455 460

Asn Ile Gln Lys Leu Ser Arg Thr Ile His Phe Ser Gly Asn Asp Phe

465 470 475 480

Arg Pro

<210> 67

<211> 470

<212> PRT

<213> 北拟杆菌(Paenibacillus borealis)

<400> 67

Met Gly Tyr Glu Leu Ile Ile Lys Asn Ala Ser Ile Pro Gln Gly Asp

1 5 10 15

Arg Gln Val Leu Thr Asp Ile Leu Val Asn Lys Gly Val Ile Val Gly

20 25 30

Phe Ala Lys Asp Ile Asp Ala Glu Ala Asp Ala Ile Ile Asp Ala Gln

35 40 45

Gly Lys Leu Val Leu Pro Gly Val Ile Asp Ser His Thr His Phe Asn

50 55 60

Asp Pro Gly Phe Thr His Arg Glu Asp Phe Val Thr Gly Thr Ser Ser

65 70 75 80

Ala Ala Ala Gly Gly Val Thr Met Ile Val Asp Met Pro Cys Cys Ser

85 90 95

Val Pro Ser Val Arg Ser Val Glu Asn Leu His Asn Lys Leu Asn Ala

100 105 110

Leu Glu Gly Lys Gly Ile Val Asp Tyr Ala Met Trp Gly Gly Ile Thr

115 120 125

Gly Glu Asp Val Arg Asn Asp Val Leu Asn Ile Val Lys Glu Gln Ala

130 135 140

Ala Glu Gly Val Val Ala Phe Lys Val Tyr Met Thr Pro Ser Val Pro

145 150 155 160

Ser Tyr Pro Arg Val Thr Asp Pro Glu Met Leu Glu Ala Phe Tyr Ala

165 170 175

Val Ala Glu Thr Gly Met Pro Ile Gly Ile His Ala Glu Asn Tyr Ala

180 185 190

Ile Cys Asp Tyr Tyr Thr Gln Lys Leu Lys Lys Glu Gly Arg Met Asp

195 200 205

Tyr Pro Ala Trp Ala Glu Ala Arg Lys Ile Leu Ala Glu Lys Ala Ala

210 215 220

Ile Gln Leu Gly Ile Ser Phe Ser Glu Glu Ser Gly Ala Arg Leu His

225 230 235 240

Ile Val His Met Ser Thr Ala Ala Gly Asn Gln Leu Ile Lys Glu Ala

245 250 255

Lys Leu Lys Gly Val Lys Val Thr Ala Glu Ser Cys Pro His Tyr Leu

260 265 270

Thr Leu Asn Ala Val Asp Ala Met Thr Glu Phe Gly Thr Phe Ala Lys

275 280 285

Ile Ala Pro Pro Leu Arg Arg Pro Glu Asp Asn Glu Ala Leu Trp Gln

290 295 300

Gly Leu Val Asp Gly Thr Ile Asp Phe Met Ala Thr Asp His Ala Pro

305 310 315 320

Tyr Glu Ile Ala Thr Glu Lys Asp Ala Pro Gly Met Asp Val Trp Thr

325 330 335

Ser Phe Pro Gly Ile Pro Gly Val Glu Thr Leu Val Pro Ile Met Ile

340 345 350

Ser Glu Gly Tyr Asn Lys Gly Arg Leu Ser Leu Ser Arg Leu Val Glu

355 360 365

Leu Leu Cys Thr Asn Pro Ala Ile His Tyr Gly Leu Tyr Pro Lys Lys

370 375 380

Gly Ala Leu Glu Ile Gly Thr Asp Ala Asp Phe Thr Ile Val Asp Leu

385 390 395 400

Glu Lys Glu Trp Thr Leu Asp Lys Asp Lys Met His Cys Lys Pro Lys

405 410 415

Tyr Thr Pro Phe His Gly Met Lys Leu Lys Gly Lys Val Asp Lys Thr

420 425 430

Ile Val Arg Gly Thr Leu Val Tyr Asp Asp Glu Leu Gly Val Val Gly

435 440 445

Lys Pro Gly Tyr Gly Glu Phe Val Lys Arg Gln Thr Ile Ser Glu Leu

450 455 460

Pro Arg Leu Leu Lys Tyr

465 470

<210> 68

<211> 514

<212> PRT

<213> Paraclostridium benzoelyticum

<400> 68

Met Asp Leu Ile Ile Lys Asn Ala Asn Ile Pro Gln Gly Asp Glu Met

1 5 10 15

Val Leu Thr Asn Ile Leu Val Lys Asp Glu Lys Ile Val Gly Phe Val

20 25 30

Asp Asp Ile Asn Met Ile Asp Ala Lys Glu Ile Ile Asp Ala Asn Lys

35 40 45

Asn Leu Val Met Pro Gly Cys Ile Asp Ser His Thr His Phe Met Tyr

50 55 60

Gln Gly Phe Pro His Arg Glu Asn Phe Leu Thr Gly Thr Ala Ala Ala

65 70 75 80

Ala Thr Gly Gly Val Thr Thr Ile Ile Asp Met Pro Cys Cys Ser Val

85 90 95

Pro Ser Val Arg Ser Val Glu Gln Leu Glu Thr Lys Leu Gly Ile Cys

100 105 110

Gly Pro Gln Ala Val Val Asp Tyr Ala Met Trp Gly Gly Val Thr Gly

115 120 125

Glu Asp Val Arg Glu Gly Trp Met Asn Asn Val Lys Glu Gln Ala Asp

130 135 140

Tyr Gly Val Val Ala Phe Lys Val Tyr Met Thr Pro Ser Val Pro Thr

145 150 155 160

Tyr Pro Arg Val Thr Asp Pro Glu Met Phe Glu Cys Phe Lys Ala Val

165 170 175

Ser Glu Thr Gly Leu Pro Ile Gly Ile His Ala Glu Asn Phe Ala Met

180 185 190

Cys Asp Phe Tyr Val Asn Lys Phe Lys Glu Glu Gly Arg Leu Asp Gly

195 200 205

Pro Ala Trp Ala Glu Ala Arg Met Glu Leu Ala Glu Lys Val Ala Ile

210 215 220

Glu Leu Gly Ile Ser Phe Ala Glu Ala Ser Lys Ala Arg Leu His Ile

225 230 235 240

Val His Met Ser Thr Gly Val Gly Ala Lys Leu Val Lys Glu Ala Lys

245 250 255

Met Arg Gly Leu Asp Val Thr Ser Glu Ser Cys Pro His Tyr Leu Thr

260 265 270

Leu Asn Tyr Gln Asp Ala Met Thr Glu Tyr Gly Pro Leu Ala Lys Ile

275 280 285

Ala Pro Pro Leu Arg Thr Lys Lys Asp Asn Val Glu His Trp Lys Gly

290 295 300

Ile Asn Asp Gly Ser Ile Asp Phe Ile Ala Thr Asp His Ala Pro Tyr

305 310 315 320

Glu Ile Glu Ser Glu Lys Leu Lys Glu Gly Thr Asn Ile Trp Thr Ser

325 330 335

Phe Pro Gly Ile Pro Gly Val Glu Thr Met Val Pro Val Ile Val Ser

340 345 350

Glu Gly Tyr Asn Lys Gly Arg Ile Ser Leu Ser Lys Leu Val Asp Ile

355 360 365

Leu Ser Thr Asn Ala Ala Lys His Tyr Gly Leu Tyr Pro Lys Lys Gly

370 375 380

Ala Leu Asn Ile Gly Ser Asp Ala Asp Phe Ala Ile Ile Asp Leu Asn

385 390 395 400

Lys Glu Trp Thr Ile Lys Asn Glu Asn Leu Val Thr Met Cys Gly Tyr

405 410 415

Thr Pro Leu Glu Gly Met Lys Leu Lys Gly Arg Ile Ile Lys Thr Ile

420 425 430

Val Arg Gly Asn Leu Val Tyr Glu Asp Gly Glu Ala Gly Val Leu Gly

435 440 445

Asn Leu Glu Asn Tyr Asp Val Leu Phe Asn Asn Pro Glu Glu Leu Thr

450 455 460

Ser Gly Glu Tyr Lys Val Ser Tyr Arg Lys Asp His Pro Glu Leu Ile

465 470 475 480

Val Glu His Val Lys Asn Ile Lys Gly Ile Lys Val Lys Pro Gly Phe

485 490 495

Gly Lys Phe Ile Lys Arg Gln Thr Ile Ser Lys Leu Asp Lys Asn Ile

500 505 510

Lys Phe

<210> 69

<211> 486

<212> PRT

<213> Bacillus solani

<400> 69

Met Glu Leu Ile Leu Lys Asn Ala Asn Ile Pro Gln Gly Asp Arg Gln

1 5 10 15

Val Leu Thr Asn Ile Leu Ile Asn Asp Gly Lys Ile Val Gly Phe Ser

20 25 30

Asn Asp Ile Ser Phe Leu Asn Ala Glu Lys Val Ile Asp Ile Gly Gly

35 40 45

Lys Leu Val Val Pro Gly Cys Ile Asp Pro His Thr His Phe Met Asp

50 55 60

Pro Gly Phe Thr His Arg Glu Thr Phe Ala Thr Gly Ser Arg Ser Ala

65 70 75 80

Val Ala Gly Gly Leu Thr Thr Ile Ile Asp Met Pro Cys Cys Ser Lys

85 90 95

Pro Ser Val Arg Asp Gly Glu Ser Phe Gln Lys Lys Ile Gly Pro Ile

100 105 110

Arg Asp Gln Ala Tyr Val Asp Tyr Cys Phe Trp Gly Gly Met Thr Gly

115 120 125

Glu Asp Ala Arg Glu Gly Trp Leu Asp Asn Val Tyr Pro Gln Ile Asp

130 135 140

Glu Gly Val Val Ala Phe Lys Val Tyr Met Thr Pro Ser Val Pro Thr

145 150 155 160

Phe Pro Arg Val Ser Asp Ala Glu Met Leu Glu Ile Phe Lys Ala Val

165 170 175

Ser Lys Thr Gly Leu Pro Ile Gly Val His Ala Glu Asn Tyr Asp Ile

180 185 190

Cys Thr Phe Asn Ser Lys Lys Leu Glu Lys Glu Gly Arg Phe Asp Gly

195 200 205

Pro Ala Trp Ala Glu Ala Arg Ser Thr Leu Ala Glu Lys Val Ala Ile

210 215 220

Glu Leu Ile Ile Ser Phe Ala Glu Ala Thr Asp Ala Arg Val His Val

225 230 235 240

Val His Met Thr Thr Lys Glu Gly Ala Glu Leu Val Lys Ala Ala Lys

245 250 255

Lys Arg Gly Val Lys Val Thr Ala Glu Thr Cys Pro His Tyr Leu Val

260 265 270

Leu Asn Ala Lys Asp Ser Met Ser Glu Arg Gly Ser Phe Ala Lys Ile

275 280 285

Ala Pro Pro Leu Arg Gly Lys Glu Asp Asn Glu Ala His Trp Gln Ala

290 295 300

Leu Ala Asp Gly Thr Ile Asp Phe Val Gly Thr Asp His Ala Pro Tyr

305 310 315 320

Glu Ile Ala Thr Glu Lys Asp Phe Pro Gly Ala Thr Ile Trp Asn Thr

325 330 335

Phe Pro Gly Ile Pro Gly Val Glu Thr Met Val Pro Ile Leu Val Ser

340 345 350

Glu Gly Leu Asn Lys Gly Arg Leu Ser Leu Ser Arg Phe Val Glu Val

355 360 365

Thr Ser Arg Asn Ala Ala Ile His Tyr Gly Ile Tyr Pro Lys Lys Gly

370 375 380

Ser Met Glu Ile Gly Ser Asp Ala Asp Phe Thr Val Ile Asp Leu Glu

385 390 395 400

Lys Glu Tyr Val Ile Asp Glu Gln Lys Thr Glu Ser Met Ala Lys Tyr

405 410 415

Asn Pro Leu His Gly Met Lys Leu Lys Gly Lys Pro Val Gln Thr Ile

420 425 430

Ile Arg Gly Asn Val Val Tyr Glu Asp Gly Lys Gly Val Leu Gly Gln

435 440 445

Ala Gly Tyr Gly Gln Tyr Val Pro Arg Gln Ser Ile Gln Arg Leu Glu

450 455 460

Arg Thr Phe Lys Tyr Glu Lys Phe Glu Ala Val Lys Val Glu Ala Lys

465 470 475 480

Glu Thr Val Lys Gln Asn

485

<210> 70

<211> 470

<212> PRT

<213> Thermohalobacter berrensis

<400> 70

Met Tyr Asp Leu Ile Leu Lys Asn Ala Arg Ile Pro Gln Gly Asp Asp

1 5 10 15

Thr Val Val Thr Asn Ile Leu Val Lys Asp Gly Lys Ile Ser Gly Phe

20 25 30

Thr Gln Glu Leu Asp Gly Ile Glu Ala Lys Glu Val Met Asp Val Glu

35 40 45

Gly His Leu Thr Leu Pro Gly Cys Ile Asp Ser His Val His Ile Asn

50 55 60

Asp Pro Gly Phe Thr His Arg Glu Asn Phe Leu Thr Gly Thr Ser Ala

65 70 75 80

Ala Ala Ser Gly Gly Ile Thr Thr Val Ile Asp Met Pro Cys Cys Ser

85 90 95

Val Pro Ser Val Arg Ser Val Glu Gln Leu Glu Asn Lys Leu Asn Ala

100 105 110

Ile Lys Asp Lys Ala Ile Val Asp Tyr Ala Met Trp Gly Gly Val Thr

115 120 125

Gly Glu Asp Val Arg Glu Gly Trp Leu His Asn Val Lys Glu Gln Ala

130 135 140

Asp Tyr Gly Val Val Ala Phe Lys Val Tyr Met Thr Pro Ser Val Pro

145 150 155 160

Thr Tyr Pro Arg Val Thr Asp Pro Glu Met Leu Glu Ala Phe Lys Ala

165 170 175

Val Ala Glu Thr Gly Leu Pro Ile Gly Ile His Ala Glu Asn Phe Asp

180 185 190

Met Cys Asp Phe Tyr Val Lys Lys Phe Glu Glu Glu Gly Arg Met Asp

195 200 205

Gly Pro Ala Trp Ala Glu Ala Arg Met Pro Leu Ala Glu Lys Val Ala

210 215 220

Ile Glu Leu Gly Ile Ser Phe Ala Glu Ala Thr Gly Ala Arg Leu His

225 230 235 240

Ile Val His Met Ser Thr Gly Ile Gly Ala Lys Leu Val Gly Glu Ala

245 250 255

Lys Lys Arg Gly Leu Asp Val Thr Ala Glu Thr Cys Pro His Tyr Leu

260 265 270

Thr Leu Asn Tyr Lys Asp Ala Met Ser Lys Phe Lys Gln Phe Ala Lys

275 280 285

Ile Ala Pro Pro Leu Arg Thr Glu Lys Asp Asn Glu Glu Leu Trp Glu

290 295 300

Gly Leu Gln Asn Gly Ser Val Asp Phe Ile Ala Thr Asp His Ala Pro

305 310 315 320

Tyr Glu Ile Ala Thr Glu Lys Asp Ala Glu Gly Met Asn Ile Trp Thr

325 330 335

Ala Phe Pro Gly Ile Pro Gly Val Glu Thr Met Val Pro Ile Ile Ile

340 345 350

Ser Glu Gly Tyr Asn Lys Gly Arg Leu Ser Leu Ser Arg Leu Val Glu

355 360 365

Val Leu Ser Thr Ser Pro Ala Lys His Tyr Gly Leu Tyr Pro Lys Lys

370 375 380

Gly Ala Met Phe Ile Gly Ser Asp Ala Asp Phe Thr Val Ile Asp Leu

385 390 395 400

Asp Lys Glu Trp Thr Ile Asp Lys Asp Lys Met Tyr Thr Lys Ser Lys

405 410 415

Tyr Thr Pro Phe His Gly Tyr Lys Leu Lys Gly Lys Pro Val Lys Thr

420 425 430

Ile Val Arg Gly Lys Leu Val Tyr Asp Asp Glu Asn Gly Ile Val Gly

435 440 445

Gln Glu Gly Tyr Gly Glu Phe Ile Lys Arg Gln Ser Ile Gln Lys Leu

450 455 460

Asp Lys Ala Ile Lys Tyr

465 470

<210> 71

<211> 514

<212> PRT

<213> 不动杆菌(Acinetobacter sp. RIT592)

<400> 71

Met Asp Leu Ile Ile Lys Asn Ala Asn Ile Pro Gln Gly Asp Glu Met

1 5 10 15

Val Leu Thr Asn Ile Leu Val Lys Asp Glu Lys Ile Val Gly Phe Val

20 25 30

Asp Asp Ile Asn Met Ile Asp Ala Lys Glu Ile Ile Asp Ala Asn Lys

35 40 45

Asn Leu Val Met Pro Gly Cys Ile Asp Ser His Thr His Phe Met Tyr

50 55 60

Gln Gly Phe Pro His Arg Glu Asn Phe Leu Thr Gly Thr Ala Ala Ala

65 70 75 80

Ala Thr Gly Gly Val Thr Thr Ile Ile Asp Met Pro Cys Cys Ser Val

85 90 95

Pro Ser Val Arg Ser Ile Glu Gln Leu Glu Thr Lys Leu Gly Ile Cys

100 105 110

Gly Pro Gln Ala Val Val Asp Tyr Ala Met Trp Gly Gly Val Thr Gly

115 120 125

Glu Asp Val Arg Glu Gly Trp Met His Asn Val Lys Glu Gln Ala Asp

130 135 140

Tyr Gly Val Val Ala Phe Lys Val Tyr Met Thr Pro Ser Val Pro Thr

145 150 155 160

Tyr Pro Arg Val Thr Asp Pro Glu Met Phe Glu Cys Phe Lys Ala Val

165 170 175

Ser Glu Thr Gly Leu Pro Ile Gly Ile His Ala Glu Asn Phe Ala Met

180 185 190

Cys Asp Phe Tyr Val Asn Lys Phe Lys Glu Glu Gly Arg Leu Asp Gly

195 200 205

Pro Ala Trp Ala Glu Ala Arg Met Glu Leu Ala Glu Lys Val Ala Ile

210 215 220

Glu Leu Gly Ile Ser Phe Ala Glu Ala Ser Lys Ala Arg Leu His Ile

225 230 235 240

Val His Met Ser Thr Gly Val Gly Ala Lys Leu Val Lys Glu Ala Lys

245 250 255

Met Arg Gly Leu Asp Val Thr Ser Glu Ser Cys Pro His Tyr Leu Thr

260 265 270

Leu Asn Tyr Gln Asp Ala Met Thr Glu Tyr Gly Pro Leu Ala Lys Ile

275 280 285

Ala Pro Pro Leu Arg Thr Lys Lys Asp Asn Ala Glu His Trp Lys Gly

290 295 300

Ile Asn Asp Gly Ser Ile Asp Phe Ile Ala Thr Asp His Ala Pro Tyr

305 310 315 320

Glu Ile Glu Ser Glu Lys Leu Lys Glu Gly Thr Asn Ile Trp Thr Ser

325 330 335

Phe Pro Gly Ile Pro Gly Val Glu Thr Met Val Pro Val Ile Val Ser

340 345 350

Glu Gly Tyr Asn Lys Gly Arg Ile Ser Leu Ser Lys Leu Val Asp Ile

355 360 365

Leu Ser Thr Asn Ala Ala Lys His Tyr Gly Leu Tyr Pro Lys Lys Gly

370 375 380

Ala Leu Asn Ile Gly Ser Asp Ala Asp Phe Ala Ile Ile Asp Leu Asn

385 390 395 400

Lys Glu Trp Thr Ile Lys Asn Glu Asn Leu Val Thr Met Cys Gly Tyr

405 410 415

Thr Pro Leu Glu Gly Met Lys Leu Lys Gly Arg Ile Ile Lys Thr Ile

420 425 430

Val Arg Gly Asn Leu Val Tyr Glu Asp Gly Glu Asp Gly Val Leu Gly

435 440 445

Lys Leu Glu Asn Tyr Asp Val Leu Phe Asn Asn Pro Glu Glu Leu Thr

450 455 460

Ser Gly Glu Tyr Lys Val Ser Tyr Arg Lys Asp His Pro Glu Leu Ile

465 470 475 480

Val Glu His Val Lys Asn Ile Lys Gly Ile Lys Val Lys Pro Gly Phe

485 490 495

Gly Lys Phe Ile Lys Arg Gln Thr Ile Ser Lys Leu Asp Lys Asn Ile

500 505 510

Lys Phe

<210> 72

<211> 471

<212> PRT

<213> Maledivibacter halophilus

<400> 72

Met Thr Tyr Asp Val Ile Val Lys Asn Ala Arg Ile Pro Gln Gly Asp

1 5 10 15

Asp Thr Val Val Thr Asn Ile Leu Val Lys Asp Gln Lys Ile Ala Gly

20 25 30

Phe Thr Met Asp Leu Asp Gly Ile Gln Ala Ser Lys Phe Ile Asp Ala

35 40 45

Glu Gly His Leu Thr Leu Pro Gly Cys Ile Asp Ser His Thr His Ile

50 55 60

Asn Asp Pro Gly Phe Thr His Arg Glu Asn Phe Leu Thr Gly Ser Ser

65 70 75 80

Ala Ala Ala Ser Gly Gly Val Thr Thr Ile Val Asp Met Pro Cys Cys

85 90 95

Ser Val Pro Ser Val Arg Ser Val Glu Asn Leu Glu His Lys Leu Asn

100 105 110

Ala Ile Arg Asp Lys Ala Ile Ile Asp Tyr Gly Met Trp Gly Gly Val

115 120 125

Thr Gly Glu Asp Val Arg Asn Gly Trp Leu His Asn Val Lys Glu Gln

130 135 140

Ala Asp Tyr Gly Val Leu Ala Phe Lys Val Tyr Met Thr Pro Ser Val

145 150 155 160

Pro Thr Tyr Pro Arg Val Thr Asp Pro Glu Met Leu Glu Ala Phe Arg

165 170 175

Ala Val Ala Glu Thr Gly Val Pro Val Gly Ile His Ala Glu Asn Phe

180 185 190

Ala Leu Cys Asp Phe Tyr Val Lys Lys Tyr Glu Lys Glu Gly Arg Met

195 200 205

Asp Pro Pro Ala Trp Ala Gln Ala Arg Ser Thr Leu Ala Glu Lys Val

210 215 220

Ala Ile Glu Leu Gly Ile Ser Phe Ala Glu Glu Thr Gly Ala Arg Leu

225 230 235 240

His Ile Val His Met Ser Ser Gly Ile Gly Ala Lys Leu Val Gly Asp

245 250 255

Ala Lys Lys Arg Gly Leu Asp Val Thr Ala Glu Thr Cys Pro His Tyr

260 265 270

Leu Thr Leu Asn Tyr Gln Asp Ala Met Thr Glu Phe Lys Gln Phe Ala

275 280 285

Lys Ile Ala Pro Pro Leu Arg Thr Lys Glu Asp Asn Glu Glu Gln Trp

290 295 300

Lys Ala Leu Gln Asp Gly Arg Ile Asp Phe Val Ala Thr Asp His Ala

305 310 315 320

Pro Tyr Glu Ile Pro Thr Glu Lys Asp Ala Glu Gly Met His Ile Trp

325 330 335

Asn Ala Phe Pro Gly Ile Pro Gly Val Glu Thr Met Val Pro Val Ile

340 345 350

Val Ser Glu Gly Tyr Asn Lys Gly Arg Leu Ser Leu Ser Arg Leu Val

355 360 365

Glu Val Leu Cys Ser Arg Pro Ala Lys His Tyr Gly Ile Tyr Pro Lys

370 375 380

Lys Gly Ala Met Phe Ile Gly Ser Asp Ala Asp Phe Thr Ile Ile Asp

385 390 395 400

Leu Asp Lys Glu Trp Thr Ile Asp Lys Asp Lys Met Tyr Thr Met Ala

405 410 415

Lys Tyr Thr Pro Phe His Gly Tyr Lys Leu Lys Gly Lys Pro Val Lys

420 425 430

Thr Ile Leu Arg Gly Lys Leu Val Tyr Asp Asp Glu Asn Gly Ile Val

435 440 445

Gly Glu Glu Gly Tyr Gly Glu Phe Val Arg Arg Gln Asn Ile Gln Lys

450 455 460

Leu Asp Lys Thr Ile Lys Tyr

465 470

<210> 73

<211> 531

<212> PRT

<213> Tissierella creatinini

<400> 73

Met Tyr Asp Val Ile Ile Lys Asn Ala Lys Ile Pro Gln Gly Asp Asn

1 5 10 15

Thr Ile Leu Thr Asn Ile Leu Val Lys Asp Glu Lys Ile Ala Gly Phe

20 25 30

Ile Asp Asn Ile Asp Asn Ile Asp Ser Lys Glu Leu Ile Asp Ala Lys

35 40 45

Gly His Leu Thr Leu Pro Gly Cys Ile Asp Ser His Thr His Phe Met

50 55 60

Tyr Gln Gly Phe Pro His Arg Glu Asn Phe Leu Thr Gly Thr Ala Ala

65 70 75 80

Ala Ala Arg Gly Gly Val Thr Thr Val Ile Asp Met Pro Cys Cys Ser

85 90 95

Val Pro Ser Val Arg Ser Val Lys Gln Leu Gln Leu Lys Val Asp Leu

100 105 110

Ile Gln Pro Gln Ala Leu Val Asp Phe Ala Leu Trp Gly Gly Val Thr

115 120 125

Gly Glu Asp Val Arg Glu Gly Trp Leu Asp Asn Val Gln Glu Gln Ala

130 135 140

Asp Phe Gly Val Val Ser Phe Lys Ala Tyr Met Thr Pro Ser Val Pro

145 150 155 160

Thr Tyr Pro Arg Val Thr Asp Pro Glu Met Tyr Glu Ala Phe Lys Ala

165 170 175

Val Ala Lys Thr Gly Leu Pro Val Gly Ile His Ala Glu Asn Tyr Ala

180 185 190

Met Cys Asp Tyr Tyr Val Lys Lys Leu Arg Glu Glu Gly Arg Thr Asp

195 200 205

Gly Pro Ala Trp Glu Glu Ala Arg Leu Glu Leu Ala Glu Gln Val Ala

210 215 220

Ile Glu Leu Gly Ile Ser Phe Ala Glu Ala Ala Asp Val Arg Leu His

225 230 235 240

Ile Val His Met Ala Leu Gly Lys Gly Ala Gln Leu Val Lys Ala Ala

245 250 255

Lys Gln Arg Gly Leu Arg Val Thr Ser Glu Thr Thr Pro His Tyr Leu

260 265 270

Thr Ile Asn Ala Lys Asp Ala Met Thr Glu His Gly Ala Tyr Ala Lys

275 280 285

Ile Ala Pro Pro Leu Arg Ser Lys Asp Ser Asn Asp Leu Leu Trp Glu

290 295 300

Gly Leu Arg Asn Gly Ser Val Asp Phe Val Ala Thr Asp His Ala Pro

305 310 315 320

Tyr Glu Ile Glu Ser Glu Lys Asp Ala Pro Gly Met Asn Ile Trp Thr

325 330 335

Ala Phe Pro Gly Ile Pro Gly Val Glu Thr Met Val Pro Ile Val Val

340 345 350

Asp Gly Tyr Asn Lys Gly Lys Ile Ser Leu Ser Thr Met Val Asn Val

355 360 365

Leu Ser Lys Asn Ala Ala Ile His Tyr Gly Leu Tyr Pro Lys Lys Gly

370 375 380

Ala Met Glu Ile Gly Ser Asp Ala Asp Phe Thr Ile Val Asp Leu Asp

385 390 395 400

Lys Glu Trp Thr Ile Asp Val Lys Asp Gln Ala Ser Met Cys Gly Tyr

405 410 415

Thr Pro Leu Glu Gly Met Lys Leu Lys Gly Arg Val Asp Lys Thr Ile

420 425 430

Val Arg Gly His Leu Val Phe Val Asp Glu Gly Glu Ser Ser Leu Ala

435 440 445

Asn Leu Thr Asp Glu Glu Leu Gln Lys Val Val His Asn Phe Pro Glu

450 455 460

Gly Val Thr Glu Lys Tyr Ala Asp Glu Phe Ala Lys Tyr Pro His Leu

465 470 475 480

Asn Ser Ala Glu Tyr Lys Asn Ser Tyr Arg Ile Asn His Pro Glu Val

485 490 495

Ile Asp Lys His Ile Arg Gly Ile Lys Gly Ile Val Ala Lys Pro Gly

500 505 510

Phe Gly Gln Trp Val Lys Arg Gln Ser Ile Gln Val Leu Pro Arg Glu

515 520 525

Ile Arg Tyr

530

<210> 74

<211> 420

<212> PRT

<213> 堆肥宏基因组(compost metagenome)

<400> 74

Met Leu Pro Gly Val Ile Asp Ser His Thr His Phe Asn Asp Pro Gly

1 5 10 15

Phe Thr His Arg Glu Asp Phe Val Thr Gly Thr Ser Ser Ala Ala Ala

20 25 30

Gly Gly Val Thr Met Ile Val Asp Met Pro Cys Cys Ser Val Pro Ser

35 40 45

Val Arg Ser Ala Asp Asn Leu Phe Asn Lys Leu Asn Ala Leu Glu Gly

50 55 60

Lys Gly Ile Val Asp Tyr Ala Met Trp Gly Gly Ile Thr Gly Glu Asp

65 70 75 80

Val Arg Asn Asn Val Leu Pro Glu Ile Ile Ser Glu Gln Ile Ala Asn

85 90 95

Gly Val Val Ala Phe Lys Val Tyr Met Thr Pro Ser Val Pro Ser Tyr

100 105 110

Pro Arg Val Thr Asp Pro Glu Met Leu Glu Ala Phe Ala Ala Val Ser

115 120 125

Pro Thr Gly Leu Pro Ile Gly Ile His Ala Glu Asn Phe Ala Ile Cys

130 135 140

Asp Tyr Phe Thr Asn Lys Leu Lys Gly Glu Gly Arg Met Asp Tyr Pro

145 150 155 160

Ala Trp Ala Glu Ala Arg Met Ala Leu Ala Glu Lys Ala Ala Ile Gln

165 170 175

Leu Gly Ile Ser Phe Ala Glu Glu Thr Gly Ala Arg Leu His Ile Val

180 185 190

His Met Ser Thr Gly Ala Gly Asn Glu Leu Ile Arg Glu Ala Lys Leu

195 200 205

Lys Gly Thr Lys Val Thr Ala Glu Ser Cys Pro His Tyr Leu Thr Leu

210 215 220

Asn Ala Val Asp Ala Met Thr Glu Phe Gly Thr Phe Ala Lys Ile Ala

225 230 235 240

Pro Pro Leu Arg Thr Ala Lys Asp Asn Glu Val Leu Trp Gln Gly Leu

245 250 255

Ala Asp Gly Thr Ile Asp Phe Met Ala Thr Asp His Ala Pro Tyr Glu

260 265 270

Ile Ala Thr Glu Lys Asp Ala Pro Gly Met Asp Val Trp Thr Ser Phe

275 280 285

Pro Gly Ile Pro Gly Val Glu Thr Leu Val Pro Ile Met Ile Ser Glu

290 295 300

Gly Tyr Asn Lys Gly Arg Leu Ser Leu Ser Arg Leu Val Glu Leu Leu

305 310 315 320

Cys Thr Asn Pro Ala Ile His Tyr Gly Leu Tyr Pro Lys Lys Gly Ala

325 330 335

Leu Glu Ile Gly Thr Asp Ala Asp Phe Thr Ile Val Asp Leu Glu Lys

340 345 350

Glu Trp Thr Leu Asp Lys Asp Gln Met His Cys Lys Pro Lys Tyr Thr

355 360 365

Pro Phe His Gly Met Lys Leu Lys Gly Lys Val Asp Lys Thr Ile Val

370 375 380

Arg Gly Thr Leu Val Tyr Asp Asp Ala Leu Gly Val Val Gly Lys Pro

385 390 395 400

Gly His Gly Glu Tyr Val Lys Arg Gln Thr Ile Ser Glu Leu Pro Arg

405 410 415

Leu Leu Lys Tyr

420

<210> 75

<211> 471

<212> PRT

<213> Natronincola peptidivorans

<400> 75

Met Lys Tyr Asp Val Val Val Lys Asn Ala Arg Ile Pro Gln Gly Asp

1 5 10 15

Asp Thr Met Leu Thr His Ile Leu Val Lys Asp Glu Lys Ile Ala Gly

20 25 30

Phe Leu Thr Asp Leu Glu Gly Ile Gln Tyr Glu Lys Val Ile Asp Ala

35 40 45

Glu Gly His Leu Thr Ile Pro Gly Cys Phe Asp Ser His Thr His Phe

50 55 60

Met Asp Pro Gly Phe Thr His Arg Glu Asn Phe Leu Thr Gly Thr Ser

65 70 75 80

Ser Ala Ala Ala Gly Gly Leu Thr Thr Ile Met Asp Met Pro Cys Cys

85 90 95

Ser Lys Pro Ser Val Arg Gly Val Glu Glu Leu Glu Ser Lys Leu Asn

100 105 110

Ala Ile Lys Asp Lys Ala Ile Ile Asp Tyr Ala Met Trp Gly Gly Val

115 120 125

Thr Gly Glu Asp Ile Arg Ala Gly Lys Lys Glu Ile Ile Lys Glu Gln

130 135 140

Ala Asp Tyr Gly Val Cys Ala Tyr Lys Val Tyr Met Thr Pro Ser Val

145 150 155 160

Pro Thr Tyr Glu Arg Val Thr Asp Pro Glu Met Leu Glu Ala Phe Arg

165 170 175

Trp Val Ala Glu Thr Gly Leu Pro Val Gly Ile His Ala Glu Asn Tyr

180 185 190

Ala Met Cys Asp Tyr Tyr Val Asn Gln Phe Gln Lys Glu Gly Arg Met

195 200 205

Asp Gly Pro Ala Trp Ala Glu Ala Arg Leu Glu Leu Ala Glu Lys Ala

210 215 220

Ala Ile Gln Leu Gly Ile Ser Phe Ala Glu Glu Thr Gly Ala Arg Leu

225 230 235 240

His Ile Val His Met Ser Thr Gly Ile Gly Ala Lys Leu Val Gly Glu

245 250 255

Ala Lys Lys Lys Gly Leu Asp Val Thr Ser Glu Thr Cys Pro His Tyr

260 265 270

Leu Met Leu Asn Tyr Gln Asp Ala Met Thr Glu His Gln Gln Tyr Ala

275 280 285

Lys Ile Ala Pro Pro Leu Arg Thr Lys Lys Asp Asn Val Glu Leu Trp

290 295 300

Glu Gly Leu Gln Asn Gly Ser Val Asp Phe Met Gly Thr Asp His Ala

305 310 315 320

Pro Tyr Glu Ile Ala Thr Glu Lys Glu Ala Glu Gly Val Asn Ile Trp

325 330 335

Thr Ala Phe Pro Gly Ile Pro Gly Val Glu Thr Leu Val Pro Ile Ile

340 345 350

Val Ser Glu Gly Tyr Asn Lys Gly Arg Leu Thr Leu Ser Lys Met Val

355 360 365

Glu Val Leu Ser Thr Asn Ala Ala Lys Gln Tyr Gly Leu Tyr Pro Lys

370 375 380

Lys Gly Ala Met His Ile Gly Ser Asp Ala Asp Phe Thr Ile Ile Asp

385 390 395 400

Leu Glu Lys Glu Trp Thr Ile Asp Gln Lys Lys Thr Tyr Ser Met Ala

405 410 415

Lys Tyr Asn Pro Leu His Gly Ile Lys Leu Lys Gly Lys Pro Val Lys

420 425 430

Thr Val Val Arg Gly Thr Leu Val Tyr Asp Asp Glu Ile Gly Ile Val

435 440 445

Gly Lys Glu Gly Phe Gly Gln Phe Val Arg Arg Gln Asn Ile Gln Lys

450 455 460

Leu Asp Arg Val Ile Lys Tyr

465 470

<210> 76

<211> 486

<212> PRT

<213> 坚强芽孢杆菌(Bacillus firmus)

<400> 76

Met Asp Leu Ile Leu Lys Asn Ala Asn Ile Pro Gln Gly Asp Arg Gln

1 5 10 15

Val Leu Thr Asn Ile Leu Val Asn Glu Gly Lys Ile Val Gly Tyr Thr

20 25 30

Asn Asp Ile Ser Phe Leu Asn Ala Ala Lys Val Ile Asp Ile Gln Gly

35 40 45

Lys Leu Val Val Pro Gly Cys Ile Asp Pro His Thr His Phe Met Asp

50 55 60

Pro Gly Phe Thr His Arg Glu Thr Phe Ala Thr Gly Ser Arg Ser Ala

65 70 75 80

Ala Ala Gly Gly Leu Thr Thr Ile Ile Asp Met Pro Cys Cys Ser Lys

85 90 95

Pro Ser Val Arg Asp Gly Asp Ser Phe Asn Lys Lys Ile Gly Pro Ile

100 105 110

Arg Asp Gln Ala Tyr Ile Asp Tyr Cys Phe Trp Gly Gly Met Thr Gly

115 120 125

Glu Asp Ala Arg Glu Gly Trp Ile Asp Asn Ile Tyr Pro Gln Ile Asp

130 135 140

Gln Gly Val Val Ala Phe Lys Val Tyr Met Thr Pro Ser Val Pro Thr

145 150 155 160

Phe Pro Lys Val Ser Asp Ala Glu Met Leu Glu Ile Phe Lys Asn Val

165 170 175

Ala Lys Thr Gly Leu Pro Ile Gly Val His Ala Glu Asn Tyr Asp Ile

180 185 190

Cys Thr Phe Asn Ser Lys Lys Leu Glu Lys Glu Gly Arg Leu Asp Gly

195 200 205

Pro Ala Trp Ala Glu Ala Arg Ser Ser Leu Ala Glu Lys Val Ala Ile

210 215 220

Glu Leu Ile Leu Ser Phe Ala Glu Ala Thr Asp Ala Arg Val His Val

225 230 235 240

Val His Met Ser Thr Lys Glu Gly Val Glu Leu Val Lys Ala Ala Lys

245 250 255

Lys Arg Gly Val Lys Val Thr Ala Glu Thr Cys Pro His Tyr Leu Val

260 265 270

Leu Asn Ala Gln Asp Ser Met Ser Glu Arg Gly Ser Tyr Ala Lys Ile

275 280 285

Ala Pro Pro Leu Arg Gly Lys Glu Asp Asn Glu Ser His Trp Gln Ala

290 295 300

Leu Ala Asp Gly Thr Ile Asp Phe Val Gly Thr Asp His Ala Pro Tyr

305 310 315 320

Glu Ile Ala Thr Glu Lys Asp Tyr Pro Gly Ala Thr Ile Trp Asn Thr

325 330 335

Phe Pro Gly Ile Pro Gly Val Glu Thr Met Val Pro Ile Leu Val Ser

340 345 350

Glu Gly Leu Asn Lys Gly Arg Ile Ser Leu Ser Arg Phe Val Glu Val

355 360 365

Thr Ser Arg Asn Ala Ala Ile His Tyr Gly Ile Tyr Pro Lys Lys Gly

370 375 380

Ser Met Glu Ile Gly Ser Asp Ala Asp Phe Thr Val Ile Asp Leu Glu

385 390 395 400

Lys Glu Tyr Val Ile Asp Glu Gln Lys Thr Glu Ser Met Ala Lys Tyr

405 410 415

Asn Pro Leu His Gly Met Lys Leu Lys Gly Lys Pro Val Gln Thr Ile

420 425 430

Ile Arg Gly Ser Val Val Phe Glu Glu Asp Lys Gly Ile Val Gly Glu

435 440 445

Ala Gly Tyr Gly Glu Tyr Val Pro Arg Gln Ser Ile Gln Arg Leu Glu

450 455 460

Arg Thr Leu Lys Tyr Glu Lys Tyr Glu Ala Val Asn Val Lys Thr Lys

465 470 475 480

Asp Pro Val Ala Gln Gln

485

<210> 77

<211> 471

<212> PRT

<213> Anaerovirgula multivorans

<400> 77

Met Lys Tyr Asp Val Ile Val Lys Asn Ala Arg Ile Pro Gln Gly Asp

1 5 10 15

Asp Thr Val Leu Thr His Ile Leu Val Lys Asp Glu Lys Ile Ala Gly

20 25 30

Phe Leu Thr Asp Leu Glu Gly Ile Glu Tyr Asp Lys Glu Ile Asn Ala

35 40 45

Glu Gly His Leu Thr Ile Pro Gly Cys Phe Asp Ser His Thr His Phe

50 55 60

Met Asp Pro Gly Phe Thr His Arg Glu Asn Phe Leu Thr Gly Thr Ser

65 70 75 80

Ser Ala Ala Ala Gly Gly Leu Thr Thr Ile Met Asp Met Pro Cys Cys

85 90 95

Ser Lys Pro Ser Val Arg Gly Val Glu Glu Leu Glu Ser Lys Leu Asn

100 105 110

Ala Ile Lys Asp Lys Ala Ile Ile Asp Tyr Ala Met Trp Gly Gly Val

115 120 125

Thr Gly Glu Asp Ile Arg Ala Gly Lys Lys Glu Ile Ile Lys Glu Gln

130 135 140

Ala Asp Tyr Gly Val Cys Ala Tyr Lys Val Tyr Met Thr Pro Ser Val

145 150 155 160

Pro Thr Tyr Glu Arg Val Thr Asp Pro Glu Met Leu Glu Ala Phe Arg

165 170 175

Trp Val Ala Glu Thr Gly Leu Pro Val Gly Ile His Ala Glu Asn Tyr

180 185 190

Ala Met Cys Asp Tyr Tyr Val Asn Lys Phe Gln Lys Glu Gly Arg Leu

195 200 205

Asp Gly Pro Ala Trp Ala Glu Ala Arg Leu Glu Leu Ala Glu Lys Ala

210 215 220

Ala Ile Gln Leu Gly Ile Ser Phe Ala Glu Glu Thr Gly Ala Arg Leu

225 230 235 240

His Ile Val His Met Ser Thr Gly Ile Gly Ala Lys Leu Val Gly Glu

245 250 255

Ala Lys Lys Lys Gly Leu Asp Val Thr Ser Glu Thr Cys Pro His Tyr

260 265 270

Leu Thr Leu Asn Tyr Gln Asp Ala Met Thr Glu His Arg Gln Tyr Ala

275 280 285

Lys Ile Ala Pro Pro Leu Arg Thr Lys Arg Asp Asn Glu Glu Leu Trp

290 295 300

Glu Gly Leu Gln Asn Gly Ser Val Asp Phe Met Gly Thr Asp His Ala

305 310 315 320

Pro Tyr Glu Ile Ala Thr Glu Lys Glu Ala Glu Gly Val Asn Ile Trp

325 330 335

Thr Ala Phe Pro Gly Ile Pro Gly Val Glu Thr Leu Val Pro Ile Ile

340 345 350

Val Ser Glu Gly Tyr Asn Lys Gly Arg Leu Thr Leu Ser Lys Met Val

355 360 365

Glu Val Leu Ser Thr Asn Ala Ala Lys Gln Tyr Gly Leu Tyr Pro Lys

370 375 380

Lys Gly Ala Met His Ile Gly Ser Asp Ala Asp Phe Thr Ile Ile Asp

385 390 395 400

Leu Glu Lys Glu Trp Thr Ile Asp Gln Lys Lys Thr Tyr Ser Met Ala

405 410 415

Lys Tyr Asn Pro Leu His Gly Ile Lys Leu Lys Gly Lys Pro Val Lys

420 425 430

Thr Val Val Arg Gly Thr Leu Val Tyr Asp Asp Glu Ile Gly Ile Val

435 440 445

Gly Glu Glu Gly Phe Gly Gln Phe Val Arg Arg Gln Ser Ile Gln Lys

450 455 460

Leu Asp Arg Val Ile Lys Tyr

465 470

<210> 78

<211> 471

<212> PRT

<213> 产氢羧基嗜热菌(Carbydothermus hydrogenoformans)

<400> 78

Met Gly Phe Asp Leu Val Leu Lys Asn Ala Lys Ile Pro Gln Gly Asn

1 5 10 15

Arg Thr Val Asp Thr His Ile Leu Val Lys Asp Gly Lys Ile Ala Gly

20 25 30

Phe Thr Asn Asp Leu Thr Gly Ile Glu Val Ala Glu Val Ile Asp Ala

35 40 45

Glu Asn Asn Leu Val Leu Pro Gly Cys Ile Asp Ser His Thr His Phe

50 55 60

Asn Asp Pro Gly Phe Thr His Arg Glu Asn Phe Ala Thr Gly Thr Gln

65 70 75 80

Ala Ala Ala Ala Gly Gly Val Thr Thr Ile Ile Asp Met Pro Cys Cys

85 90 95

Ser Ile Pro Ser Val Arg Ser Val Asp Asn Leu Glu Ala Lys Leu Glu

100 105 110

Val Ile Lys Asp Lys Ala Tyr Val Asp Tyr Ala Met Trp Gly Gly Ala

115 120 125

Thr Gly Glu Asp Val Arg Asn Asn Ala Leu Tyr Asn Ile Lys Glu Gln

130 135 140

Ala Asp Tyr Gly Val Val Ala Phe Lys Val Tyr Met Thr Pro Ser Val

145 150 155 160

Pro Thr Tyr Pro Pro Ala Ser Asp Ala Glu Met Leu Glu Ile Phe Arg

165 170 175

Glu Val Ala Lys Thr Gly Leu Pro Val Gly Ile His Ala Glu Asn Leu

180 185 190

Asp Leu Cys Thr Tyr His Val Asn Lys Leu Arg Asn Glu Gly Arg Phe

195 200 205

Asp Gly Pro Ala Trp Ala Glu Ala Arg Leu Glu Leu Ala Glu Lys Val

210 215 220

Ala Ile Gln Met Ala Ile Ser Phe Ala Glu Glu Thr Gly Ala Arg Leu

225 230 235 240

His Ile Val His Met Ser Thr Gly Ile Gly Gly Val Leu Val Gly Glu

245 250 255

Ala Lys Lys Arg Gly Leu Asp Val Thr Ala Glu Thr Cys Pro His Tyr

260 265 270

Leu Val Leu Asn Ala Tyr Glu Ser Met Thr Lys Trp Arg Gln Phe Ala

275 280 285

Lys Ile Ala Pro Pro Leu Arg Thr Thr Arg Asp Asn Glu Met Leu Trp

290 295 300

Glu Gln Leu Ala Asp Gly Arg Val Asp Phe Ile Ala Thr Asp His Ala

305 310 315 320

Pro Tyr Glu Ile Ala Thr Glu Lys Glu Ala Glu Gly Met Asp Ile Trp

325 330 335

Thr Ala Phe Pro Gly Ile Pro Gly Val Glu Thr Met Val Pro Ile Ile

340 345 350

Ile Ser Glu Gly Tyr Asn Lys Gly Arg Leu Ser Leu Ser Arg Leu Val

355 360 365

Asp Val Leu Ser Arg Asn Ala Ala Ile His Tyr Gly Leu Tyr Pro Lys

370 375 380

Lys Gly Ala Leu Glu Ile Gly Ser Asp Ala Asp Phe Thr Val Ile Asp

385 390 395 400

Leu Asp Tyr Glu Trp Thr Ile Asp Lys Asp Lys Met Tyr Thr Met Ala

405 410 415

Lys Tyr Thr Pro Phe His Gly Met Lys Leu Lys Gly Lys Pro Val Lys

420 425 430

Thr Ile Val Arg Gly Lys Leu Val Tyr Asp Asp Lys Asn Gly Ile Val

435 440 445

Gly Lys Pro Gly Tyr Gly Gln Phe Val Lys Arg Gln Thr Ile Ser Lys

450 455 460

Leu Glu Arg Trp Ile Lys Phe

465 470

<210> 79

<211> 470

<212> PRT

<213> 产乙酸互营单包菌(Sporanaerobacter acetigenes)

<400> 79

Met Tyr Asp Val Ile Val Lys Asn Ala Arg Ile Pro Gln Gly His Asp

1 5 10 15

Thr Val Leu Thr Asn Ile Leu Val Lys Asp Gly Lys Ile Ala Gly Phe

20 25 30

Val Glu Ser Ile Glu Gly Leu Glu Gly Arg Glu Ile Ile Asp Ala Lys

35 40 45

Gly Asn Leu Thr Ile Pro Gly Cys Ile Asp Ser His Thr His Phe Met

50 55 60

Asp Pro Gly Phe Thr His Arg Glu Asn Phe Leu Thr Gly Thr Ser Gly

65 70 75 80

Ala Ala Ala Gly Gly Val Thr Thr Ile Ile Asp Met Pro Cys Cys Ser

85 90 95

Val Pro Ser Val Arg Ser Val Ala Gln Leu Glu Asn Lys Ile Glu Lys

100 105 110

Ile Lys Asp Lys Ala Ile Val Asp Phe Ala Leu Trp Gly Gly Val Thr

115 120 125

Gly Glu Asp Val Arg Glu Gly Trp Leu His Asn Val Gln Glu Gln Ala

130 135 140

Asp Tyr Gly Val Thr Gly Phe Lys Val Tyr Met Thr Pro Ser Val Pro

145 150 155 160

Thr Tyr Pro Arg Val Thr Asp Pro Glu Met Tyr Glu Cys Phe Lys Ala

165 170 175

Val Ala Lys Thr Gly Leu Pro Val Gly Val His Ala Glu Asn Phe Ala

180 185 190

Met Cys Asp Phe Tyr Val Asn Arg Phe Gln Glu Glu Gly Arg Met Asp

195 200 205

Gly Pro Ala Trp Ala Glu Ala Arg Met Ile Leu Ala Glu Lys Val Ala

210 215 220

Ile Gln Leu Ala Ile Ser Phe Ala Glu Asp Thr Gly Val Arg Leu His

225 230 235 240

Ile Val His Met Ser Thr Gly Ile Gly Ala Asp Leu Val Lys Glu Ala

245 250 255

Lys Met Arg Gly Leu Asp Val Thr Ala Glu Thr Cys Pro His Tyr Leu

260 265 270

Thr Leu Asn Tyr Glu Glu Thr Met Ser Lys Tyr Val Gln Leu Ala Lys

275 280 285

Ile Ala Pro Pro Ile Arg Thr Thr Glu Asp Asn Glu Arg Leu Trp Lys

290 295 300

Gly Ile Gln Glu Gly Ser Val Asp Phe Ile Ala Thr Asp His Ala Pro

305 310 315 320

Tyr Ser Leu Glu Thr Glu Lys Leu Lys Glu Gly Phe Asn Ile Trp Thr

325 330 335

Ala Phe Pro Gly Ile Pro Gly Val Glu Thr Met Ala Pro Ile Ile Ile

340 345 350

Ser Glu Gly Tyr Asn Lys Gly Arg Ile Ser Leu Ser Arg Leu Val Glu

355 360 365

Ile Leu Ser Thr Asn Ala Ala Lys His Tyr Gly Leu Tyr Pro Lys Lys

370 375 380

Gly Ile Met Asn Ile Gly Ser Asp Ala Asp Phe Thr Ile Val Asp Ile

385 390 395 400

Asp Lys Glu Trp Thr Ile Glu Lys Asp Lys Met Tyr Cys Leu Asn Lys

405 410 415

Tyr Thr Pro Phe Asp Gly Phe Lys Leu Lys Gly Lys Ile Tyr Lys Thr

420 425 430

Ile Val Arg Gly Lys Val Val Tyr Glu Asp Gly Glu Gly Ile Val Gly

435 440 445

Glu Glu Gly Tyr Gly Gln Phe Val Lys Arg Gln Ser Ile Gln Lys Leu

450 455 460

Asp His Ile Ile Lys Phe

465 470

<210> 80

<211> 532

<212> PRT

<213> Proteiniborus sp.

<400> 80

Met Tyr Asp Leu Ile Leu Lys Asn Ala Arg Ile Pro Gln Gly Asp Tyr

1 5 10 15

Thr Val Leu Thr Asn Ile Leu Val Lys Asp Glu Lys Ile Ala Gly Phe

20 25 30

Thr Asp Cys Ile Asp Gly Ile Glu Ala Lys Asn Thr Ile Asp Ala His

35 40 45

Gly His Leu Thr Leu Pro Gly Cys Ile Asp Ser His Thr His Phe Met

50 55 60

Tyr Gln Gly Phe Pro His Arg Glu Asn Phe Leu Thr Gly Thr Ala Ala

65 70 75 80

Ala Ala Ala Gly Gly Ile Thr Thr Val Ile Asp Met Pro Cys Cys Ser

85 90 95

Val Pro Ser Val Arg Ser Val Asp Gln Leu Asn Leu Lys Lys Asp Leu

100 105 110

Cys Ala Pro Gln Ala Leu Val Asp Phe Ala Leu Trp Gly Gly Val Thr

115 120 125

Gly Glu Asp Val Arg Glu Asn Arg Leu His Asn Val Gln Glu Gln Ala

130 135 140

Asp Ala Gly Val Val Ala Phe Lys Val Tyr Met Thr Pro Ser Val Pro

145 150 155 160

Thr Tyr Pro Arg Val Thr Asp Pro Glu Met Tyr Glu Cys Phe Lys Ala

165 170 175

Val Ala Lys Thr Gly Leu Pro Ile Gly Ile His Ala Glu Asn Phe Ala

180 185 190

Met Cys Asp Phe Tyr Val Lys Lys Phe Arg Glu Glu Gly Arg Met Asp

195 200 205

Gly Pro Ala Trp Ala Glu Ala Arg Met Glu Leu Ala Glu Lys Val Ala

210 215 220

Ile Glu Leu Gly Ile Ser Phe Ala Glu Ala Ser Gly Ala Arg Leu His

225 230 235 240

Ile Val His Met Ser Thr Gly Ile Gly Ala Lys Leu Val Gly Glu Ala

245 250 255

Lys Lys Arg Gly Leu Asn Val Thr Ser Glu Ser Cys Pro His Tyr Leu

260 265 270

Thr Leu Asn Tyr Gln Glu Ser Met Thr Ile His Gly Ala Asn Ala Lys

275 280 285

Ile Ala Pro Pro Leu Arg Thr Lys Arg Asp Asn Glu Glu Gln Trp Glu

290 295 300

Gly Ile Arg Asn Gly Ser Ile Asp Phe Ile Ala Thr Asp His Ala Pro

305 310 315 320

Tyr Glu Leu Glu Ser Glu Lys Ile Lys Glu Gly Thr Thr Ile Trp Thr

325 330 335

Ala Phe Pro Gly Ile Pro Gly Val Glu Thr Met Val Pro Val Ile Val

340 345 350

Ser Glu Gly Tyr Asn Lys Gly Arg Ile Ser Leu Ser Lys Leu Val Asp

355 360 365

Ile Leu Ser Thr Asn Ala Ala Lys His Tyr Gly Leu Tyr Pro Lys Lys

370 375 380

Gly Ala Leu Asn Ile Gly Ser Asp Ala Asp Phe Thr Ile Ile Asp Leu

385 390 395 400

Asp Lys Thr Trp Thr Ile Asp Pro Lys Lys Gln Ala Ser Met Cys Gly

405 410 415

Tyr Thr Pro Leu Glu Gly Met Glu Leu Lys Gly Lys Val Val Lys Thr

420 425 430

Ile Val Arg Gly His Leu Val Tyr Val Asp Glu Asp Ala Ser Val Leu

435 440 445

Ser Glu Met Thr Asp Glu Asp Leu Lys Lys Leu Val His Asp Tyr Pro

450 455 460

Glu Gly Val Ala Glu Lys Tyr Lys Glu His Phe Asp Leu Tyr Pro Gln

465 470 475 480

Leu Tyr Ser Ala Glu Tyr Glu Asn Ser Tyr Arg Lys Asn His Pro Glu

485 490 495

Val Ile Asp Gln His Ile Arg Ser Ile Arg Gly Ile Lys Val Lys Pro

500 505 510

Gly Phe Gly Lys Phe Val Lys Arg Gln Ser Ile Gln Val Leu Pro Arg

515 520 525

Thr Ile Thr Tyr

530

<210> 81

<211> 486

<212> PRT

<213> 芽孢杆菌(Bacillus sp.)

<400> 81

Met Asp Leu Ile Leu Lys Asn Ala Asn Ile Pro Gln Gly Asp Arg Gln

1 5 10 15

Val Leu Thr Asn Ile Leu Val Asn Glu Gly Lys Ile Val Gly Tyr Thr

20 25 30

Asn Asp Ile Ser Phe Leu Asn Ala Ser Lys Val Ile Asp Ile Gln Gly

35 40 45

Lys Leu Val Val Pro Gly Cys Ile Asp Pro His Thr His Phe Met Asp

50 55 60

Pro Gly Phe Thr His Arg Glu Thr Phe Ala Thr Gly Ser Arg Ser Ala

65 70 75 80

Ala Ala Gly Gly Leu Thr Thr Ile Ile Asp Met Pro Cys Cys Ser Lys

85 90 95

Pro Ser Val Arg Asp Gly Asp Ser Phe Asn Lys Lys Ile Gly Pro Ile

100 105 110

Arg Asp Gln Ala Tyr Ile Asp Tyr Cys Phe Trp Gly Gly Met Thr Gly

115 120 125

Glu Asp Ala Arg Glu Gly Trp Ile Asp Asn Ile Tyr Pro Gln Ile Asp

130 135 140

Gln Gly Val Val Ala Phe Lys Val Tyr Met Thr Pro Ser Val Pro Thr

145 150 155 160

Phe Pro Lys Val Ser Asp Ala Glu Met Leu Glu Ile Phe Lys Asn Val

165 170 175

Ala Lys Thr Gly Leu Pro Ile Gly Val His Ala Glu Asn Tyr Asp Ile

180 185 190

Cys Thr Phe Asn Ser Lys Lys Leu Glu Lys Glu Gly Arg Leu Asp Gly

195 200 205

Pro Ala Trp Ala Glu Ala Arg Ser Ser Leu Ala Glu Lys Val Ala Ile

210 215 220

Glu Leu Ile Leu Ser Phe Ala Glu Ala Thr Asp Ala Arg Val His Val

225 230 235 240

Val His Met Ser Thr Lys Glu Gly Val Glu Leu Val Lys Ala Ala Lys

245 250 255

Lys Arg Gly Val Lys Val Thr Ala Glu Thr Cys Pro His Tyr Leu Val

260 265 270

Leu Asn Ala Lys Asp Ser Met Ser Glu Arg Gly Ser Tyr Ala Lys Ile

275 280 285

Ala Pro Pro Leu Arg Gly Lys Glu Asp Asn Glu Ser His Trp Gln Ala

290 295 300

Leu Ala Asp Gly Thr Ile Asp Phe Val Gly Thr Asp His Ala Pro Tyr

305 310 315 320

Glu Ile Ala Thr Glu Lys Asp Tyr Pro Gly Ala Thr Ile Trp Asn Thr

325 330 335

Phe Pro Gly Ile Pro Gly Val Glu Thr Met Val Pro Ile Leu Val Ser

340 345 350

Glu Gly Leu Asn Lys Gly Arg Leu Ser Leu Ser Arg Phe Val Glu Val

355 360 365

Thr Ser Arg Asn Ala Ala Ile His Tyr Gly Ile Tyr Pro Lys Lys Gly

370 375 380

Ser Met Glu Ile Gly Ser Asp Ala Asp Phe Thr Val Ile Asp Leu Glu

385 390 395 400

Lys Glu Tyr Val Ile Asp Glu Gln Lys Thr Glu Ser Met Ala Lys Tyr

405 410 415

Asn Pro Leu His Gly Met Lys Leu Lys Gly Lys Pro Val Gln Thr Ile

420 425 430

Ile Arg Gly Asn Val Val Phe Glu Glu Asp Lys Gly Val Val Gly Glu

435 440 445

Ala Gly Tyr Gly Glu Tyr Val Pro Arg Gln Ser Ile Gln Arg Leu Glu

450 455 460

Arg Thr Phe Lys Tyr Glu Lys Tyr Glu Ala Val Asn Val Lys Thr Lys

465 470 475 480

Asp Pro Val Ala Gln Gln

485

<210> 82

<211> 465

<212> PRT

<213> [Clostridium] ultunense Esp

<400> 82

Met Tyr Gln Val Ile Val Lys Asn Ala Arg Ile Pro Gln Gly Asp Glu

1 5 10 15

Thr Val Leu Thr Asn Ile Leu Ile Lys Asp Glu Arg Ile Ala Gly Phe

20 25 30

Val Asp Gly Ile Asp Gly Ile Glu Ala Glu Glu Ile Ile Asp Ala Glu

35 40 45

Gly His Leu Thr Leu Pro Gly Cys Ile Asp Ser His Thr His Phe Met

50 55 60

Asp Pro Gly Phe Thr His Arg Glu Asn Phe Leu Thr Gly Thr Ser Ser

65 70 75 80

Ala Ala Ala Gly Gly Leu Thr Thr Ile Val Asp Met Pro Cys Cys Ser

85 90 95

Val Pro Ser Val Arg Ser Ile Pro Glu Leu Glu Asn Lys Leu Glu Lys

100 105 110

Ile Lys Asp Lys Ala Ile Ile Asp Tyr Gly Phe Trp Gly Gly Val Thr

115 120 125

Gly Glu Asp Val Arg Glu Gly Trp Leu His Asn Val Lys Glu Gln Ala

130 135 140

Asp Tyr Gly Val Ile Gly Phe Lys Val Tyr Met Thr Pro Ser Val Pro

145 150 155 160

Thr Tyr Pro Arg Val Thr Asp Pro Glu Met Tyr Glu Ala Phe Arg Thr

165 170 175

Val Ala Glu Thr Gly Leu Pro Ile Gly Ile His Ala Glu Asn Phe Ala

180 185 190

Met Cys Asp Tyr Phe Val Lys Lys Phe Gln Lys Glu Gly Arg Met Glu

195 200 205

Gly Pro Ala Trp Ala Glu Ala Arg Met Glu Leu Ala Glu Lys Val Ala

210 215 220

Ile Gln Leu Gly Ile Ser Phe Ala Glu Asp Thr Gly Ala Arg Leu His

225 230 235 240

Ile Val His Met Ser Thr Gly Ile Gly Ala Lys Leu Val Lys Glu Ala

245 250 255

Lys Leu Lys Gly Leu Asp Val Thr Ser Glu Thr Cys Pro His Tyr Leu

260 265 270

Thr Leu Asn Tyr Glu Glu Ala Met Ser Glu Tyr Gly Ala Phe Ala Lys

275 280 285

Ile Ala Pro Pro Leu Arg Thr Lys Arg Asp Asn Lys Glu Leu Trp Glu

290 295 300

Gly Leu Arg Asp Gly Ser Val Asp Phe Ile Ala Thr Asp His Ala Pro

305 310 315 320

Tyr His Ile Glu Asp Glu Lys Ala Lys Lys Gly Met Asn Ile Trp Thr

325 330 335

Ala Phe Pro Gly Ile Pro Gly Val Glu Thr Met Val Pro Ile Ile Val

340 345 350

Ser Glu Gly Tyr Asn Lys Gly Arg Ile Ser Leu Ser Lys Met Val Glu

355 360 365

Ile Leu Ser Thr Asn Ala Ala Ile His Tyr Gly Leu Tyr Pro Lys Lys

370 375 380

Gly Ser Leu Asn Ile Gly Ala Asp Ala Asp Leu Thr Ile Ile Asp Leu

385 390 395 400

Asp Lys Glu Trp Ala Ile Asp Gln Glu Lys Thr Tyr Ser Met Ala Lys

405 410 415

Tyr Asn Pro Leu His Gly Leu Lys Leu Lys Gly Lys Val Ala Lys Thr

420 425 430

Val Val Arg Gly Lys Val Val Phe Asp Glu Lys Glu Gly Ile Thr Val

435 440 445

Lys Glu Gly Tyr Gly Gln Phe Val Lys Arg Thr Glu Tyr Thr Lys Ile

450 455 460

Arg

465

<210> 83

<211> 493

<212> PRT

<213> Virgibacillus indicus

<400> 83

Met Asp Leu Ile Leu Lys Asn Ala Val Ile Pro Gln Gly Asp Arg Lys

1 5 10 15

Val Glu Thr Asn Ile Leu Ile Asp Asn Gly Glu Ile Val Gly Phe Thr

20 25 30

Asn Ala Val Asn Gly Leu Ala Ala Asp Glu Ile Ile Asp Ile Lys Gly

35 40 45

Asn Leu Val Val Pro Gly Cys Ile Asp Pro His Thr His Phe Met Asp

50 55 60

Pro Gly Phe Thr His Arg Glu Thr Phe Thr Thr Gly Thr Arg Ser Ala

65 70 75 80

Ala Ala Gly Gly Ile Thr Thr His Ile Asp Met Pro Cys Cys Ser Lys

85 90 95

Pro Ser Val Arg Asp Lys Glu Ser Phe His Lys Lys Ile Glu Pro Leu

100 105 110

Arg Asp Gln Ala Tyr Ile Asp Tyr Cys Phe Trp Gly Gly Met Thr Gly

115 120 125

Glu Asp Val Arg Glu Gly Leu Leu Asp Asn Ile Tyr Pro Gln Ile Glu

130 135 140

Glu Gly Val Val Ala Phe Lys Val Tyr Met Thr Pro Ser Val Pro Thr

145 150 155 160

Phe Pro Lys Val Thr Asp Ala Glu Met Leu Glu Ile Phe Ser Lys Ile

165 170 175

Ala Pro Thr Gly Met Pro Ile Gly Val His Ala Glu Asn Tyr Glu Ile

180 185 190

Cys Thr Phe Tyr Thr Glu Lys Met Lys Lys Glu Gly Arg Met Asp Gly

195 200 205

Pro Ala Trp Ala Glu Ala Arg Met Pro Leu Ala Glu Lys Val Ala Ile

210 215 220

Gln Leu Ile Ile Ser Phe Ala Glu Ala Thr Gly Ala Arg Val His Val

225 230 235 240

Val His Met Thr Thr Lys Glu Gly Val Glu Leu Val Arg Glu Ala Lys

245 250 255

Lys Arg Gly Val Lys Val Thr Ala Glu Thr Cys Pro His Tyr Leu Val

260 265 270

Leu Asn Ala Gln Asp Ser Met Thr Glu Arg Gly Thr Phe Ala Lys Ile

275 280 285

Ala Pro Pro Leu Arg Glu Lys Glu Asp Asn Ile Val His Trp Gln Gly

290 295 300

Leu Ala Asp Gly Thr Ile Asp Phe Val Gly Thr Asp His Ala Pro Tyr

305 310 315 320

Glu Ile Glu Thr Glu Lys Asp Ala Pro Asp Ser Asp Ile Trp Asn Ser

325 330 335

Phe Pro Gly Ile Pro Gly Val Glu Thr Met Val Pro Ile Leu Val Ser

340 345 350

Glu Gly Leu Asn Lys Gly Arg Leu Ser Leu Ser Arg Phe Val Glu Val

355 360 365

Thr Ser Arg Asn Ala Ala Ile His Tyr Gly Ile Tyr Pro Lys Lys Gly

370 375 380

Ser Met Glu Leu Gly Ser Asp Ala Asp Phe Thr Val Ile Asp Leu Asp

385 390 395 400

Arg Glu Tyr Thr Ile Asp Glu Gln Lys Thr Glu Ser Met Ala Lys Tyr

405 410 415

Asn Pro Leu His Gly Met Glu Leu Lys Gly Lys Pro Ile Gln Thr Ile

420 425 430

Val Arg Gly Lys Val Val Tyr Asp Glu Asp Asn Gly Gly Ile Val Gly

435 440 445

Lys Ser Gly Tyr Gly Glu Tyr Val Lys Arg Gln Ser Ile Gln Arg Leu

450 455 460

Asp Arg Thr Leu Lys Tyr Glu Val Tyr Glu Glu Asn Ala Lys Glu Ala

465 470 475 480

Glu Ser Gln Pro Arg Arg Glu Lys Ala Leu Ile Asn Asn

485 490

<210> 84

<211> 471

<212> PRT

<213> 类芽孢杆菌(Paenibacillus sp. )

<400> 84

Met Ser Tyr Asp Leu Ile Ile Lys Asn Ala Asn Ile Pro Gln Gly Asp

1 5 10 15

Arg Gln Val Leu Thr Asp Ile Leu Val Lys Asn Gly Val Ile Ala Gly

20 25 30

Phe Ala Lys Gly Ile Lys Ala Asp Ala Ala Glu Val Ile Asp Ala Lys

35 40 45

Gly Met Leu Val Leu Pro Gly Val Ile Asp Ser His Thr His Phe Asn

50 55 60

Asp Pro Gly Phe Thr His Arg Glu Asp Phe Val Thr Gly Thr Ser Ser

65 70 75 80

Ala Ala Ala Gly Gly Val Thr Met Ile Val Asp Met Pro Cys Cys Ser

85 90 95

Val Pro Ser Val Arg Ser Ala Asp Asn Leu Phe Asn Lys Leu Asn Ala

100 105 110

Leu Glu Gly Lys Gly Ile Val Asp Tyr Ala Met Trp Gly Gly Ile Thr

115 120 125

Gly Glu Asp Val Arg Asn Asn Val Leu Pro Glu Ile Ile Ser Glu Gln

130 135 140

Val Ala Asn Gly Val Val Ala Phe Lys Val Tyr Met Thr Pro Ser Val

145 150 155 160

Pro Ser Tyr Pro Arg Val Thr Asp Pro Glu Met Leu Glu Ala Phe Ala

165 170 175

Ala Val Ser Pro Thr Gly Leu Pro Ile Gly Ile His Ala Glu Asn Phe

180 185 190

Ala Ile Cys Asp Tyr Phe Thr Gln Lys Leu Lys Gly Glu Gly Arg Met

195 200 205

Asp Tyr Pro Ala Trp Ala Glu Ala Arg Met Ala Leu Ala Glu Lys Ala

210 215 220

Ala Ile Gln Leu Gly Ile Ser Phe Ala Glu Glu Thr Gly Ala Arg Leu

225 230 235 240

His Ile Val His Met Ser Thr Ala Ala Gly Asn Glu Leu Ile Arg Glu

245 250 255

Ala Lys Leu Lys Gly Thr Lys Val Thr Ala Glu Ser Cys Pro His Tyr

260 265 270

Leu Thr Leu Asn Ala Val Asp Ala Met Thr Glu Phe Gly Thr Phe Ala

275 280 285

Lys Ile Ala Pro Pro Leu Arg Thr Ala Lys Asp Asn Glu Ala Leu Trp

290 295 300

Gln Gly Leu Val Asp Gly Thr Ile Asp Phe Met Ala Thr Asp His Ala

305 310 315 320

Pro Tyr Glu Ile Ala Thr Glu Lys Asn Ala Pro Gly Met Asp Val Trp

325 330 335

Thr Ser Phe Pro Gly Ile Pro Gly Val Glu Thr Leu Val Pro Ile Met

340 345 350

Ile Ser Glu Gly Tyr Asn Lys Gly Arg Leu Ser Leu Ser Arg Leu Val

355 360 365

Glu Leu Leu Cys Thr Asn Pro Ala Val His Tyr Gly Leu Tyr Pro Lys

370 375 380

Lys Gly Ala Leu Glu Ile Gly Thr Asp Ala Asp Phe Thr Ile Val Asp

385 390 395 400

Leu Glu Lys Glu Trp Thr Leu Asp Lys Asp Gln Met His Cys Lys Pro

405 410 415

Lys Tyr Thr Pro Phe His Gly Met Lys Leu Lys Gly Lys Val Asp Lys

420 425 430

Thr Ile Val Arg Gly Thr Leu Val Phe Asp Glu Ala Leu Gly Val Ile

435 440 445

Gly Lys Pro Gly His Gly Glu Tyr Val Lys Arg Gln Thr Ile Ser Glu

450 455 460

Leu Pro Arg Leu Leu Lys Tyr

465 470

<210> 85

<211> 552

<212> PRT

<213> Andreesenia angusta

<400> 85

Met Tyr Asp Leu Ile Ile Lys Asn Ala Lys Ile Pro Gln Gly Asp Glu

1 5 10 15

Thr Val Met Thr Asn Ile Leu Val Leu Glu Glu Lys Ile Ala Gly Phe

20 25 30

Val Asp Ser Thr His Gly Ala Gln Ala Lys Glu Val Ile Asp Ala Glu

35 40 45

Gly His Leu Val Phe Pro Gly Phe Met Asp Ser His Thr His Ile Met

50 55 60

Tyr His Gly Phe Gly His Arg Glu Asn Trp Val Thr Gly Ser Ala Ala

65 70 75 80

Ala Val Arg Gly Gly Ile Ser Thr Ile Ile Asp Met Pro Cys Cys Thr

85 90 95

Val Pro Ser Thr Arg Thr Val Lys Gln Leu Glu Leu Lys Ile Asp Ile

100 105 110

Ala Asn Ala Gln Thr Val Val Asp Tyr Gly Leu Trp Gly Gly Val Thr

115 120 125

Gly Glu Asp Val Arg Glu Gly Trp Met His Asn Val Gln Glu Gln Ala

130 135 140

Asp Phe Gly Val Val Ala Phe Lys Val Tyr Met Thr Pro Ser Val Pro

145 150 155 160

Thr Tyr Pro Arg Val Thr Asp Pro Glu Met Leu Glu Cys Phe Lys Glu

165 170 175

Val Ala Lys Thr Gly Leu Pro Ile Gly Ile His Ala Glu Asn Phe Ala

180 185 190

Met Cys Asp Phe Tyr Val Lys Lys Phe Gln Ala Asp Gly Arg Met Asp

195 200 205

Gly Pro Ala Trp Ser Glu Ala Arg Asn Glu Leu Ala Glu Lys Val Ala

210 215 220

Ile Glu Leu Gly Ile Ser Phe Ala Glu Ala Thr Gly Ala Arg Leu His

225 230 235 240

Ile Val His Met Ser Thr Gly Ile Gly Ala Lys Leu Ile Gly Glu Ala

245 250 255

Lys Lys Arg Gly Ile Asn Ala Thr Ser Glu Ser Cys Pro His Tyr Leu

260 265 270

Thr Leu Asn Tyr Gln Asp Ala Met Thr Lys His Gly Ala Cys Ala Lys

275 280 285

Ile Ala Pro Pro Ile Arg Thr Ser Gly Asp Asn Glu Glu His Trp Glu

290 295 300

Gly Leu Arg Asn Gly Ser Ile Asp Phe Met Ala Thr Asp His Ala Pro

305 310 315 320

Tyr Glu Leu Glu Ser Glu Lys Thr Ser Glu Gly Thr Thr Ile Trp Asn

325 330 335

Ala Phe Pro Gly Ile Pro Gly Thr Glu Thr Met Ala Pro Ile Ile Ile

340 345 350

Ser Glu Gly Leu Asn Lys Gly Arg Leu Ser Leu Ser Arg Thr Val Glu

355 360 365

Val Leu Ala Thr Asn Ala Ala Lys His Tyr Gly Leu Tyr Pro Lys Lys

370 375 380

Gly Ala Met Met Val Gly Ser Asp Ala Asp Phe Ala Ile Val Asp Leu

385 390 395 400

Ser Lys Glu Trp Thr Ile Asp Pro Lys Thr Gln Ala Ser Met Cys Gly

405 410 415

Tyr Thr Pro Leu Glu Gly Met Lys Leu Lys Gly Arg Ile Ile Lys Thr

420 425 430

Ile Ile Arg Gly Thr Leu Val Phe Asp Asp Ala Gln Glu Gly Val Leu

435 440 445

Pro Thr Leu Thr Asp Ala Glu Leu Asp Lys Ile Val His Asn Phe Pro

450 455 460

Glu Gly Cys Ala Glu Lys Phe Ala Asp Ile Phe Glu Gln Phe Pro Glu

465 470 475 480

Leu Ala Ser Ala Glu Tyr Asp Arg Ser Tyr Arg Lys Leu His Pro Glu

485 490 495

Lys Ile Asn Glu Asp Ile Arg Arg Ile Lys Gly Ile Met Val Lys Pro

500 505 510

Gly Phe Gly Lys Phe Val Lys Arg Gln Ser Ile Gln Val Leu Pro Lys

515 520 525

Thr Ile Thr Tyr Asn Thr Thr Thr Pro Thr Gly Val Asn Arg Arg Glu

530 535 540

Arg Glu Asp Leu Pro Glu Phe Arg

545 550

<210> 86

<211> 471

<212> PRT

<213> 类芽孢杆菌 (Paenibacillus sp.)

<400> 86

Met Ser Tyr Asp Leu Ile Ile Lys Asn Ala Ser Ile Pro Gln Gly Asp

1 5 10 15

Arg Gln Val Leu Thr Asp Ile Leu Val Asn Asn Gly Val Ile Val Gly

20 25 30

Phe Ala Lys Asp Ile Glu Ala Asp Ala Ala Glu Val Ile Asp Ala Gly

35 40 45

Gly Lys Leu Val Leu Pro Gly Val Ile Asp Ser His Thr His Phe Asn

50 55 60

Asp Pro Gly Phe Thr His Arg Glu Asp Phe Val Thr Gly Thr Ala Ser

65 70 75 80

Ala Ala Ala Gly Gly Val Thr Met Ile Val Asp Met Pro Cys Cys Ser

85 90 95

Val Pro Ser Val Arg Ser Ala Asp Asn Leu Phe Asn Lys Leu Asn Ala

100 105 110

Leu Glu Gly Lys Gly Ile Val Asp Tyr Ala Met Trp Gly Gly Ile Thr

115 120 125

Gly Glu Asp Val Arg Asn Asn Val Leu Pro Glu Ile Ile Ser Glu Gln

130 135 140

Val Ala Asn Gly Val Val Ala Phe Lys Val Tyr Met Thr Pro Ser Val

145 150 155 160

Pro Ser Tyr Pro Arg Val Thr Asp Pro Glu Met Leu Glu Ala Phe Ala

165 170 175

Ala Val Ser Pro Thr Gly Leu Pro Ile Gly Ile His Ala Glu Asn Phe

180 185 190

Ala Val Cys Asp Tyr Phe Thr Asn Lys Leu Lys Gly Glu Gly Arg Leu

195 200 205

Asp Tyr Pro Ala Trp Ala Glu Ala Arg Met Ala Leu Ala Glu Lys Ala

210 215 220

Ala Ile Gln Leu Gly Ile Ser Phe Ala Glu Glu Thr Gly Ala Arg Leu

225 230 235 240

His Ile Val His Met Ser Thr Gly Ala Gly Asn Glu Leu Ile Arg Glu

245 250 255

Ala Lys Leu Lys Gly Thr Lys Val Thr Ala Glu Ser Cys Pro His Tyr

260 265 270

Leu Thr Leu Asn Ala Val Asp Ala Met Thr Glu Phe Gly Thr Phe Ala

275 280 285

Lys Ile Ala Pro Pro Leu Arg Thr Ala Lys Asp Asn Glu Val Leu Trp

290 295 300

Gln Gly Leu Ala Asp Gly Thr Ile Asp Phe Met Ala Thr Asp His Ala

305 310 315 320

Pro Tyr Glu Ile Ala Thr Glu Lys Asp Ala Pro Gly Met Asp Val Trp

325 330 335

Thr Ser Phe Pro Gly Ile Pro Gly Val Glu Thr Leu Val Pro Ile Met

340 345 350

Ile Ser Glu Gly Tyr Asn Lys Gly Arg Leu Ser Leu Ser Arg Leu Val

355 360 365

Glu Leu Leu Cys Thr Asn Pro Ala Val His Tyr Gly Leu Tyr Pro Lys

370 375 380

Lys Gly Ala Leu Glu Ile Gly Thr Asp Ala Asp Phe Thr Ile Val Asp

385 390 395 400

Leu Glu Lys Glu Trp Thr Leu Asp Lys Asp Gln Met His Cys Lys Pro

405 410 415

Lys Tyr Thr Pro Phe His Gly Met Lys Leu Lys Gly Lys Val Asp Lys

420 425 430

Thr Ile Val Arg Gly Thr Leu Val Tyr Asp Glu Ala Leu Gly Val Val

435 440 445

Gly Lys Pro Gly His Gly Glu Tyr Val Lys Arg Gln Thr Ile Ser Glu

450 455 460

Leu Pro Arg Leu Leu Lys Tyr

465 470

<210> 87

<211> 473

<212> PRT

<213> Paludifilum halophilum

<400> 87

Met Asn Leu Leu Leu Lys Asn Ala Asn Ile Pro Gln Gly Asp Arg Gln

1 5 10 15

Val Lys Ala Asp Ile Leu Val Arg Asp Gly Lys Ile Thr Gly Val Thr

20 25 30

Ser Asp Ala Asp Gly Phe Ala Ala Asp Arg Val Ile Asp Val Asp Gly

35 40 45

Lys Leu Val Val Pro Gly Cys Ile Asp Pro His Thr His Phe Met Asp

50 55 60

Pro Gly Phe Thr His Arg Glu Thr Phe Thr Thr Gly Ser Arg Ser Ala

65 70 75 80

Ala Ala Gly Gly Leu Thr Cys His Val Asp Met Pro Cys Cys Ser Lys

85 90 95

Pro Ser Val Arg Ser Lys Gly Glu Leu His Gln Lys Leu Asn Pro Leu

100 105 110

Lys Asp Gln Ala Tyr Ile Asp Phe Ala Phe Trp Gly Gly Met Thr Gly

115 120 125

Glu Asp Val Arg Glu Gly Lys Leu Asp Asn Val Trp Gly Gln Val Glu

130 135 140

Glu Gly Val Thr Ala Phe Lys Val Tyr Met Thr Pro Ser Val Pro Thr

145 150 155 160

Phe Pro Lys Val Thr Asp Ala Glu Met Leu Glu Ile Phe Tyr Lys Ile

165 170 175

Ala Glu Thr Gly Leu Pro Val Gly Ile His Ala Glu Asn Phe Asp Ile

180 185 190

Cys Ser Phe Tyr Thr Glu Lys Leu Lys Asn Met Gly Arg Thr Asp Ala

195 200 205

Pro Ala Trp Ala Glu Ala Arg Met Ala Leu Ala Glu Lys Thr Ala Ile

210 215 220

Gln Leu Ile Ile Ala Phe Ser Glu Glu Thr Gly Ala Arg Val His Ile

225 230 235 240

Val His Met Ser Thr Lys Glu Gly Ala Glu Leu Val Arg Asp Ala Lys

245 250 255

Arg Arg Gly Val Lys Ile Thr Ala Glu Ala Cys Pro His Tyr Leu Thr

260 265 270

Leu Asn Ala Glu Glu His Met Thr Arg Phe Gly Thr Leu Ala Lys Ile

275 280 285

Ala Pro Pro Leu Arg Gly Thr Glu Asp Asn Glu Val His Trp Gln Ala

290 295 300

Leu Ala Asp Gly Thr Ile Asp Phe Val Gly Thr Asp His Ala Pro Tyr

305 310 315 320

Glu Ile Ala Thr Glu Lys Val Gly Glu Gly Leu Asp Ile Trp Asn Ser

325 330 335

Phe Pro Gly Ile Pro Gly Val Glu Thr Met Val Pro Val Met Val Ser

340 345 350

Glu Gly Leu Asn Lys Gly Arg Leu Ser Leu Ser Arg Phe Val Glu Val

355 360 365

Thr Ser Arg Asn Ala Ala Ile His Phe Gly Leu Tyr Pro Lys Lys Gly

370 375 380

Ser Met Glu Leu Gly Ser Asp Ala Asp Phe Thr Val Ile Asp Leu Glu

385 390 395 400

Arg Glu Tyr Val Val Asp Gln Glu Lys Val Gln Ser Met Ala Lys Tyr

405 410 415

Thr Pro Phe His Gly Met Lys Leu Lys Gly Lys Pro Val Met Thr Ile

420 425 430

Val Arg Gly Glu Val Val Tyr Asp Glu Asp Asn Gly Gly Ile Val Gly

435 440 445

Cys Ser Gly Tyr Gly Gln Phe Val Thr Arg Gln Ser Asn Ala Gln Leu

450 455 460

Asn Arg Val Glu Gly Glu Leu Val Tyr

465 470

<210> 88

<211> 531

<212> PRT

<213> Proteiniborus ethanoligenes

<400> 88

Met Tyr Asp Met Ile Leu Lys Asn Ala Arg Ile Pro Gln Gly Asp Asn

1 5 10 15

Thr Val Leu Thr Asn Ile Leu Val Lys Asp Glu Lys Ile Ala Gly Phe

20 25 30

Thr Asp Cys Val Asp Gly Ile Glu Ala Lys Asn Val Ile Asp Ala Gly

35 40 45

Gly His Leu Thr Leu Pro Gly Cys Ile Asp Ser His Thr His Phe Met

50 55 60

Tyr Gln Gly Phe Pro His Arg Glu Asn Phe Leu Thr Gly Thr Ala Ala

65 70 75 80

Ala Ala Gly Gly Ile Thr Thr Ile Ile Asp Met Pro Cys Cys Ser Val

85 90 95

Pro Ser Ala Arg Ser Val Asp Glu Leu Lys Leu Lys Ile Asp Val Cys

100 105 110

Gln Pro Gln Ala Leu Val Asp Phe Ala Met Trp Gly Gly Val Thr Gly

115 120 125

Glu Asp Val Arg Glu Gly Trp Leu His Asn Val Gln Glu Gln Ala Asp

130 135 140

Tyr Gly Val Val Ala Phe Lys Val Tyr Met Thr Pro Ser Val Pro Thr

145 150 155 160

Tyr Pro Arg Val Thr Asp Pro Glu Met Tyr Glu Ala Phe Arg Ala Val

165 170 175

Ala Lys Thr Gly Leu Pro Ile Gly Ile His Ala Glu Asn Phe Ala Met

180 185 190

Cys Asp Tyr Tyr Val Lys Glu Phe Gln Lys Glu Gly Arg Met Asp Gly

195 200 205

Pro Ala Trp Ala Glu Ala Arg Met Glu Leu Ala Glu Lys Val Ala Ile

210 215 220

Glu Leu Gly Ile Ser Phe Ala Glu Ala Ser Gly Ala Arg Leu His Ile

225 230 235 240

Val His Met Ser Thr Gly Ile Gly Ala Lys Leu Val Gly Glu Ala Lys

245 250 255

Lys Arg Gly Leu Asn Val Thr Ser Glu Ser Cys Pro His Tyr Leu Thr

260 265 270

Leu Asn Tyr Gln Glu Ser Met Thr Ile His Gly Ala Asn Ala Lys Ile

275 280 285

Ala Pro Pro Leu Arg Thr Lys Arg Asp Asn Glu Glu Gln Trp Glu Gly

290 295 300

Ile Arg Asn Gly Ser Ile Asp Phe Ile Ala Thr Asp His Ala Pro Tyr

305 310 315 320

Glu Leu Glu Ser Glu Lys Ile Lys Glu Gly Leu Asn Ile Trp Thr Ala

325 330 335

Phe Pro Gly Ile Pro Gly Val Glu Thr Met Val Pro Val Ile Val Ser

340 345 350

Glu Gly Tyr Asn Lys Gly Arg Ile Ser Leu Ser Lys Leu Val Asp Ile

355 360 365

Leu Ser Thr Asn Ala Ala Lys His Tyr Gly Leu Tyr Pro Lys Lys Gly

370 375 380

Ala Leu His Ile Gly Ser Asp Ala Asp Phe Thr Ile Ile Asp Leu Asp

385 390 395 400

Lys Arg Trp Val Ile Glu Pro Lys Lys Gln Ala Ser Met Cys Gly Tyr

405 410 415

Thr Pro Leu Glu Gly Met Glu Leu Gln Gly Arg Val Glu Lys Thr Ile

420 425 430

Val Arg Gly His Leu Val Tyr Lys Asp Val Asp Glu Ser Thr Leu Ala

435 440 445

Ser Leu Thr Asp Glu Glu Leu Lys Thr Ile Val His Glu Tyr Pro Glu

450 455 460

Gly Val Arg Glu Arg Tyr Pro Glu Ile Phe Lys Asn Phe Pro Asn Leu

465 470 475 480

Tyr Ser Ala Glu Tyr Glu Arg Ser Tyr Arg Lys Glu His Pro Glu Val

485 490 495

Ile Asp Lys His Val Arg Gly Ile Arg Gly Ile Gln Val Lys Pro Gly

500 505 510

Phe Gly Arg Phe Val Lys Arg Gln Ser Ile Gln Val Leu Pro Arg Thr

515 520 525

Ile Thr Tyr

530

<210> 89

<211> 471

<212> PRT

<213> Alkaliphilus metalliredigens

<400> 89

Met Lys Tyr Asp Val Ile Val Lys Asn Ala Arg Ile Pro Gln Gly Asp

1 5 10 15

Asp Thr Ile Val Thr Asn Ile Leu Val Lys Asp Glu Lys Ile Ala Gly

20 25 30

Phe Leu Thr Asp Ile Glu Gly Val Glu Phe Asp Lys Thr Ile Asp Ala

35 40 45

Glu Gly His Leu Thr Ile Pro Gly Cys Phe Asp Ser His Thr His Phe

50 55 60

Met Asp Pro Gly Phe Thr His Arg Glu Asn Phe Leu Thr Gly Ser Ser

65 70 75 80

Ser Ala Ala Ala Gly Gly Leu Thr Met Ile Met Asp Met Pro Cys Cys

85 90 95

Ser Lys Pro Ser Val Arg Ser Ile Pro Glu Leu Glu Ser Lys Ile Asn

100 105 110

Asn Ile Lys Glu Lys Ala Ile Ile Asp Phe Ala Leu Trp Gly Gly Val

115 120 125

Thr Gly Glu Asp Val Arg Glu Gly Lys Lys His Ile Val Gln Asp Gln

130 135 140

Ala Asp Tyr Gly Val Cys Ala Phe Lys Ala Tyr Met Thr Pro Ser Val

145 150 155 160

Pro Thr Tyr Glu Arg Val Thr Asp Pro Glu Met Leu Glu Ala Phe Arg

165 170 175

Trp Val Ala Glu Thr Gly Leu Pro Val Gly Ile His Ala Glu Asn Tyr

180 185 190

Ala Met Cys Asp Phe Tyr Val Lys Lys Phe Gln Ala Glu Gly Arg Met

195 200 205

Asp Gly Pro Ala Trp Ala Glu Ala Arg Leu Val Glu Ala Glu Gln Ala

210 215 220

Ala Ile Glu Leu Gly Ile Asn Phe Ala Glu Ser Ala Gly Ala Arg Leu

225 230 235 240

His Ile Val His Met Ser Ser Gly Ile Gly Ala Lys Leu Val Gly Ala

245 250 255

Ala Lys Lys Arg Gly Leu Asp Val Thr Ser Glu Thr Cys Pro His Tyr

260 265 270

Leu Met Leu Asn Tyr Gln Asp Ala Met Thr Glu His Gly Ala Phe Ala

275 280 285

Lys Ile Ala Pro Pro Leu Arg Thr Thr Lys Asp Asn Lys Glu Leu Trp

290 295 300

Glu Gly Leu Gln Asn Gly Ser Val Asp Phe Met Gly Thr Asp His Ala

305 310 315 320

Pro Tyr Glu Ile Glu Thr Glu Lys Asp Ala Glu Gly Met Asn Ile Trp

325 330 335

Thr Ala Phe Pro Gly Ile Pro Gly Val Glu Thr Leu Val Pro Ile Met

340 345 350

Val Ser Glu Gly Tyr Asn Lys Gly Arg Leu Ser Leu Ser Lys Leu Val

355 360 365

Asp Val Leu Ser Thr Asn Ala Ala Lys Gln Tyr Gly Leu Tyr Pro Lys

370 375 380

Lys Gly Ala Met His Ile Gly Ser Asp Ala Asp Phe Thr Ile Ile Asp

385 390 395 400

Leu Glu Arg Glu Trp Thr Ile Asp Gln Lys Lys Thr Tyr Ser Met Ala

405 410 415

Lys Tyr Asn Pro Leu His Gly Ile Lys Leu Lys Gly Lys Pro Val Lys

420 425 430

Thr Val Val Arg Gly Thr Val Val Tyr Asp Asp Ala Ile Gly Ile Val

435 440 445

Gly Lys Glu Gly Phe Gly Gln Phe Val Lys Arg Gln Asn Ile Gln Lys

450 455 460

Leu Asp Arg Val Ile Lys Tyr

465 470

<210> 90

<211> 490

<212> PRT

<213> 盐渍土假芽胞杆菌(Fictibacillus solisalsi)

<400> 90

Met Asp Leu Ile Leu Lys Asn Ala Met Ile Pro Gln Gly Asp Arg Thr

1 5 10 15

Val Ile Thr Asn Ile Leu Val His Glu Gly Arg Ile Thr Gly Phe Cys

20 25 30

Asn Asp Ile Gln Thr Phe Asn Ala Asp Lys Val Leu Asp Met Glu Gly

35 40 45

Lys Leu Val Val Pro Gly Cys Ile Asp Pro His Thr His Phe Met Asp

50 55 60

Pro Gly Phe Thr His Arg Glu Thr Phe Ala Thr Gly Ser Arg Ser Ala

65 70 75 80

Ala Ala Gly Gly Leu Thr Thr Ile Ile Asp Met Pro Cys Cys Ser Lys

85 90 95

Pro Ser Val Arg Asp Gly Glu Ser Phe Gln Lys Lys Ile Gly Pro Ile

100 105 110

Arg Gly Gln Ala Tyr Ile Asp Tyr Cys Phe Trp Gly Gly Met Thr Gly

115 120 125

Glu Asp Ala Arg Asn Gly Trp Ile Asp Asn Ile Tyr Pro Gln Ile Asp

130 135 140

Glu Gly Val Val Ala Phe Lys Val Tyr Met Thr Pro Ser Val Pro Thr

145 150 155 160

Phe Pro Lys Val Ser Asp Gly Glu Met Leu Glu Ile Phe Gln Lys Val

165 170 175

Ala Lys Thr Gly Met Pro Ile Gly Val His Ala Glu Asn Tyr Asp Ile

180 185 190

Cys Thr Phe Tyr Thr Glu Lys Leu Lys Asn Glu Gly Arg Glu Asp Gly

195 200 205

Pro Ala Trp Ala Glu Ala Arg Met Ala Leu Ala Glu Lys Val Ala Ile

210 215 220

Glu Leu Ile Ile Ser Tyr Ala Glu Ala Thr Asp Ala Arg Val His Val

225 230 235 240

Val His Met Ser Thr Lys Glu Gly Val Lys Leu Val Arg Glu Ala Lys

245 250 255

Lys Arg Gly Val Lys Val Thr Ser Glu Thr Cys Pro His Tyr Leu Met

260 265 270

Leu Asn Ala Gln Asp Ser Met Thr Glu Arg Gly Thr Phe Ala Lys Ile

275 280 285

Ala Pro Pro Leu Arg Gly Lys Asp Asp Asn Gln Ala His Trp Gln Ala

290 295 300

Leu Ala Asp Gly Thr Ile Asp Phe Val Gly Thr Asp His Ala Pro Tyr

305 310 315 320

Glu Ile Ala Thr Glu Lys Ala Ala Ala Asp Ser Ser Ile Trp Asn Ser

325 330 335

Phe Pro Gly Ile Pro Gly Val Glu Thr Met Val Pro Ile Leu Val Ser

340 345 350

Glu Gly Leu Asn Lys Gly Arg Leu Ser Leu Ser Arg Phe Val Glu Val

355 360 365

Thr Ser Lys Asn Ala Ala Val His Tyr Gly Ile Tyr Pro Lys Lys Gly

370 375 380

Ala Met Glu Ile Gly Ser Asp Ala Asp Phe Thr Val Ile Asp Leu Asp

385 390 395 400

His Glu Trp Ile Ile Asp Glu Lys Gln Thr Glu Ser Met Ala Lys Tyr

405 410 415

Asn Pro Leu His Gly Ile Lys Leu Lys Gly Arg Pro Val Gln Thr Ile

420 425 430

Val Arg Gly Thr Val Val Tyr Asp Tyr Glu Lys Gly Gly Ile Thr Gly

435 440 445

Thr Ala Gly Tyr Gly Glu Tyr Val Lys Arg Gln Ser Ile Gln Arg Leu

450 455 460

Glu Arg Thr Leu Lys Tyr Glu Asn Tyr Glu Ile Ala Asn Leu Pro Ser

465 470 475 480

Ala Ala Arg Gln Gln Leu Glu Lys Ser Lys

485 490

<210> 91

<211> 486

<212> PRT

<213> Sporosarcina globispora

<400> 91

Met Asp Leu Ile Leu Lys Asn Ala Asn Ile Pro Gln Gly Asp Arg Gln

1 5 10 15

Val Leu Thr Asn Ile Leu Val Asn Glu Gly Lys Ile Val Gly Cys Thr

20 25 30

Asn Asp Ile Ser Phe Leu Asn Ala Ala Lys Val Ile Asp Ile Gln Gly

35 40 45

Lys Leu Val Val Pro Gly Cys Ile Asp Pro His Thr His Phe Met Asp

50 55 60

Pro Gly Phe Thr His Arg Glu Thr Phe Ala Thr Gly Ser Arg Ser Ala

65 70 75 80

Ala Ala Gly Gly Leu Thr Thr Ile Ile Asp Met Pro Cys Cys Ser Lys

85 90 95

Pro Ser Val Arg Asp Gly Glu Ser Phe Asn Lys Lys Ile Gly Pro Ile

100 105 110

Arg Asp Gln Ala Tyr Ile Asp Tyr Cys Phe Trp Gly Gly Met Thr Gly

115 120 125

Glu Asp Ala Arg Glu Gly Trp Ile Asn Asn Ile Tyr Pro Gln Ile Asp

130 135 140

Gln Gly Val Val Ala Phe Lys Val Tyr Met Thr Pro Ser Val Pro Thr

145 150 155 160

Phe Pro Lys Val Ser Asp Ala Glu Met Leu Glu Ile Phe Lys Asn Val

165 170 175

Ala Lys Thr Gly Leu Pro Ile Gly Val His Ala Glu Asn Tyr Asp Ile

180 185 190

Cys Thr Phe Asn Ser Lys Lys Leu Glu Lys Glu Gly Arg Leu Asp Gly

195 200 205

Pro Ala Trp Ala Glu Ala Arg Ser Ser Leu Ala Glu Lys Val Ala Ile

210 215 220

Glu Leu Ile Leu Ser Phe Ala Glu Ala Thr Asp Ala Arg Val His Val

225 230 235 240

Val His Met Ser Thr Lys Glu Gly Val Glu Leu Val Lys Ala Ala Lys

245 250 255

Lys Arg Gly Val Lys Val Thr Ala Glu Thr Cys Pro His Tyr Leu Val

260 265 270

Leu Asn Ala Gln Asp Ser Met Ser Glu Arg Gly Ser Tyr Ala Lys Ile

275 280 285

Ala Pro Pro Leu Arg Gly Lys Glu Asp Asn Glu Ser His Trp Gln Ala

290 295 300

Leu Ala Asp Gly Thr Ile Asp Phe Val Gly Thr Asp His Ala Pro Tyr

305 310 315 320

Glu Ile Ala Thr Glu Lys Asp Tyr Pro Gly Ala Thr Ile Trp Asn Thr

325 330 335

Phe Pro Gly Ile Pro Gly Val Glu Thr Met Val Pro Ile Leu Val Ser

340 345 350

Glu Gly Leu Asn Lys Gly Arg Leu Ser Leu Ser Arg Phe Val Glu Val

355 360 365

Thr Ser Arg Asn Ala Ala Val His Tyr Gly Ile Tyr Pro Lys Lys Gly

370 375 380

Ser Met Glu Ile Gly Ser Asp Ala Asp Phe Thr Val Ile Asp Leu Glu

385 390 395 400

Lys Glu Tyr Val Ile Asp Glu Gln Lys Thr Glu Ser Met Ala Lys Tyr

405 410 415

Asn Pro Leu His Gly Met Lys Leu Lys Gly Lys Pro Val Gln Thr Ile

420 425 430

Ile Arg Gly Asn Val Ile Tyr Glu Glu Asp Lys Gly Ile Val Gly Glu

435 440 445

Ala Gly Tyr Gly Glu Tyr Val Pro Arg Gln Ser Ile Gln Arg Leu Glu

450 455 460

Arg Thr Leu Lys Tyr Glu Lys Tyr Glu Ala Val Asn Val Lys Ala Lys

465 470 475 480

Asp Pro Val Ala Gln Gln

485

<210> 92

<211> 486

<212> PRT

<213> 坚强芽孢杆菌(Bacillus firmus)

<400> 92

Met Asp Leu Ile Leu Lys Asn Ala Asn Ile Pro Gln Gly Asp Arg Gln

1 5 10 15

Val Leu Thr Asn Ile Leu Val Asn Lys Gly Lys Ile Val Gly Tyr Thr

20 25 30

Asn Asp Ile Ser Phe Leu Asn Ala Ala Arg Val Ile Asp Ile Lys Gly

35 40 45

Lys Leu Val Val Pro Gly Cys Ile Asp Pro His Thr His Phe Met Asp

50 55 60

Pro Gly Phe Thr His Arg Glu Thr Phe Ala Thr Gly Ser Arg Ser Ala

65 70 75 80

Ala Ala Gly Gly Leu Thr Thr Ile Ile Asp Met Pro Cys Cys Ser Lys

85 90 95

Pro Ser Val Arg Asp Gly Glu Ser Phe Asn Lys Lys Ile Gly Pro Ile

100 105 110

Arg Asp Gln Ala Tyr Ile Asp Tyr Cys Phe Trp Gly Gly Met Thr Gly

115 120 125

Glu Asp Ala Arg Glu Gly Trp Ile Asp Asn Ile Tyr Pro Gln Ile Asp

130 135 140

Gln Gly Val Val Ala Phe Lys Val Tyr Met Thr Pro Ser Val Pro Thr

145 150 155 160

Phe Pro Lys Val Ser Asp Ala Glu Met Leu Glu Ile Phe Ser Asn Val

165 170 175

Ala Lys Thr Gly Leu Pro Ile Gly Val His Ala Glu Asn Tyr Asp Ile

180 185 190

Cys Thr Phe Tyr Ser Lys Lys Leu Glu Lys Glu Gly Arg Leu Asp Gly

195 200 205

Pro Ala Trp Ala Glu Ala Arg Ser Ser Leu Ala Glu Lys Val Ala Ile

210 215 220

Glu Leu Ile Leu Ser Phe Ala Glu Ala Thr Asp Ala Arg Val His Val

225 230 235 240

Val His Met Ser Thr Lys Glu Gly Val Glu Leu Val Lys Ala Ala Lys

245 250 255

Lys Arg Gly Val Lys Val Thr Ala Glu Thr Cys Pro His Tyr Leu Val

260 265 270

Leu Asn Ala Lys Asp Ser Met Ser Glu Arg Gly Ser Tyr Ala Lys Ile

275 280 285

Ala Pro Pro Leu Arg Gly Lys Glu Asp Asn Glu Ser His Trp Gln Ala

290 295 300

Leu Ala Asp Gly Thr Ile Asp Phe Val Gly Thr Asp His Ala Pro Tyr

305 310 315 320

Glu Ile Ala Thr Glu Lys Asp Tyr Pro Gly Ala Thr Ile Trp Asn Thr

325 330 335

Phe Pro Gly Ile Pro Gly Val Glu Thr Met Val Pro Ile Leu Val Ser

340 345 350

Glu Gly Leu Asn Lys Gly Arg Leu Ser Leu Ser Arg Phe Val Glu Val

355 360 365

Thr Ser Arg Asn Ala Ala Ile His Tyr Gly Ile Tyr Pro Lys Lys Gly

370 375 380

Ser Met Glu Ile Gly Ser Asp Ala Asp Phe Thr Val Ile Asp Leu Glu

385 390 395 400

Lys Glu Tyr Val Ile Asp Glu Gln Lys Thr Glu Ser Met Ala Lys Tyr

405 410 415

Asn Pro Leu His Gly Met Lys Leu Lys Gly Lys Pro Val Gln Thr Ile

420 425 430

Ile Arg Gly Asn Val Ile Tyr Asp Glu Asp Lys Gly Ile Val Gly Glu

435 440 445

Ala Gly Tyr Gly Glu Tyr Val Pro Arg Gln Ser Ile Gln Arg Leu Glu

450 455 460

Arg Thr Leu Lys Tyr Glu Lys Tyr Glu Ala Val Lys Ala Lys Thr Lys

465 470 475 480

Asp Pro Val Ala Gln Gln

485

<210> 93

<211> 486

<212> PRT

<213> 类芽孢杆菌(Paenibacillus sp. )

<400> 93

Met Asp Leu Ile Leu Lys Asn Ala Asn Ile Pro Gln Gly Asp Arg Gln

1 5 10 15

Val Leu Thr Asn Ile Leu Val Asn Glu Gly Lys Ile Val Gly Tyr Thr

20 25 30

Asn Asp Ile Ser Phe Leu Asn Ala Ser Lys Val Ile Asp Ile Gln Gly

35 40 45

Lys Leu Val Val Pro Gly Cys Ile Asp Pro His Thr His Phe Met Asp

50 55 60

Pro Gly Phe Thr His Arg Glu Thr Phe Ala Thr Gly Ser Arg Ser Ala

65 70 75 80

Ala Ala Gly Gly Leu Thr Thr Ile Ile Asp Met Pro Cys Cys Ser Lys

85 90 95

Pro Ser Val Arg Asp Gly Asp Ser Phe Asn Lys Lys Ile Gly Pro Ile

100 105 110

Arg Asp Gln Ala Tyr Ile Asp Tyr Cys Phe Trp Gly Gly Met Thr Gly

115 120 125

Glu Asp Ala Arg Glu Gly Trp Ile Asp Asn Ile Tyr Pro Gln Ile Asp

130 135 140

Gln Gly Val Val Ala Phe Lys Val Tyr Met Thr Pro Ser Val Pro Thr

145 150 155 160

Phe Pro Lys Val Ser Asp Ala Glu Met Leu Glu Ile Phe Lys Asn Val

165 170 175

Ala Lys Thr Gly Leu Pro Ile Gly Val His Ala Glu Asn Tyr Asp Ile

180 185 190

Cys Thr Phe Asn Ser Lys Lys Leu Glu Lys Glu Gly Arg Leu Asp Gly

195 200 205

Pro Ala Trp Ala Glu Ala Arg Ser Ser Leu Ala Glu Lys Val Ala Ile

210 215 220

Glu Leu Ile Leu Ser Phe Ala Glu Ala Thr Asp Ala Arg Val His Val

225 230 235 240

Val His Met Ser Thr Lys Glu Gly Val Glu Leu Val Lys Ala Ala Lys

245 250 255

Lys Arg Gly Val Lys Val Thr Ala Glu Thr Cys Pro His Tyr Leu Val

260 265 270

Leu Asn Ala Lys Asp Ser Met Ser Glu Arg Gly Ser Tyr Ala Lys Ile

275 280 285

Ala Pro Pro Leu Arg Gly Lys Glu Asp Asn Glu Ser His Trp Gln Ala

290 295 300

Leu Ala Asp Gly Thr Ile Asp Phe Val Gly Thr Asp His Ala Pro Tyr

305 310 315 320

Glu Ile Ala Thr Glu Lys Asp Tyr Pro Gly Ala Thr Ile Trp Asn Thr

325 330 335

Phe Pro Gly Ile Pro Gly Val Glu Thr Met Val Pro Ile Leu Val Ser

340 345 350

Glu Gly Leu Asn Lys Gly Arg Leu Ser Leu Ser Arg Phe Val Glu Val

355 360 365

Thr Ser Arg Asn Ala Ala Ile His Tyr Gly Ile Tyr Pro Lys Lys Gly

370 375 380

Ser Met Glu Ile Gly Ser Asp Ala Asp Phe Thr Val Ile Asp Leu Glu

385 390 395 400

Lys Glu Tyr Val Ile Asp Glu Gln Lys Thr Glu Ser Met Ala Lys Tyr

405 410 415

Asn Pro Leu His Gly Met Lys Leu Lys Gly Lys Pro Val Gln Thr Ile

420 425 430

Ile Arg Gly Asn Val Val Phe Glu Glu Asp Lys Gly Ile Val Gly Glu

435 440 445

Ala Gly Tyr Gly Glu Tyr Val Pro Arg Gln Ser Ile Gln Arg Leu Glu

450 455 460

Arg Thr Phe Lys Phe Glu Lys Tyr Glu Ala Val Asn Val Lys Thr Lys

465 470 475 480

Asp Pro Val Ala Gln Gln

485

<210> 94

<211> 470

<212> PRT

<213> 嗜碱菌(Alkaliphilus sp.)

<400> 94

Met Tyr Asp Val Leu Ile Thr Asn Ala Arg Ile Pro Gln Gly Asp Asp

1 5 10 15

Thr Lys Leu Thr Asn Ile Leu Val Arg Asp Gly Ile Ile Glu Gly Phe

20 25 30

Val Gln Asp Ile Glu Gly Leu Gln Ala Lys Glu Ile Ile Asp Ala Glu

35 40 45

Gly His Leu Thr Leu Pro Gly Cys Ile Asp Pro His Thr His Ile Asn

50 55 60

Asp Pro Gly Phe Thr His Arg Glu Asn Phe Phe Thr Gly Thr Ser Ala

65 70 75 80

Ala Ala Thr Gly Gly Ile Thr Met Ile Ile Asp Met Pro Cys Cys Ser

85 90 95

Lys Pro Ser Val Arg Ser Val Glu Gln Leu Glu Ser Lys Val Asn Ala

100 105 110

Ile Lys Asn Lys Ala Ile Val Asp Tyr Gly Met Trp Gly Gly Val Thr

115 120 125

Gly Glu Asp Val Arg Asn Asp Trp Leu Phe Asn Val Arg Glu Gln Ala

130 135 140

Asp Phe Gly Val Leu Ala Phe Lys Val Tyr Met Thr Pro Ser Val Pro

145 150 155 160

Thr Tyr Pro Arg Val Ser Asp Pro Glu Met Leu Glu Ala Phe Lys Ala

165 170 175

Val Ser Glu Thr Gly Leu Val Val Gly Val His Ser Glu Ile Tyr Ala

180 185 190

Ile Cys Glu Tyr Tyr Thr Lys Lys Phe Glu Arg Glu Gly Arg Thr Asp

195 200 205

Ala Pro Ala Trp Ala Glu Ala Arg Lys Ala Ile Ser Glu Lys Leu Ala

210 215 220

Ile Gln Met Gly Ile Tyr Tyr Ala Glu Glu Val Gly Ala Arg Phe Gln

225 230 235 240

Val Val His Met Ala Leu Gly Glu Gly Ala Arg Met Val Arg Glu Ala

245 250 255

Lys Met Arg Gly Val Asp Val Val Ala Glu Thr Cys Pro His Tyr Leu

260 265 270

Leu Phe Asn Tyr Gln Glu Ser Met Thr Lys His Gly Val Phe Ala Lys

275 280 285

Ile Ala Pro Pro Leu Arg Thr Lys Lys Asp Asn Glu Leu Leu Trp Lys

290 295 300

Gly Leu Gln Asp Gly Ser Ile Asp Phe Ile Gly Thr Asp His Ala Pro

305 310 315 320

Tyr Glu Ile Ala Thr Glu Lys Thr Ala Ala Gly Leu His Ile Trp Asn

325 330 335

Ala Phe Pro Gly Ile Pro Gly Val Glu Thr Met Val Pro Val Met Val

340 345 350

Ser Glu Gly Tyr Asn Lys Gly Arg Leu Ser Leu Ser Arg Leu Val Glu

355 360 365

Leu Leu Cys Lys Asn Ala Ala Ile Asn Phe Gly Ile Tyr Pro Lys Lys

370 375 380

Gly Ser Met Phe Val Gly Thr Asp Ala Asp Phe Thr Ile Ile Asp Leu

385 390 395 400

Asp Arg Glu Trp Thr Ile Lys Asn Glu Glu Met Val Thr Met Ala Lys

405 410 415

Tyr Gly Pro Phe Asp Gly Met Lys Leu Lys Gly Lys Pro Val Lys Thr

420 425 430

Ile Val Arg Gly Lys Leu Val Tyr Asp Asp Glu Lys Gly Ile Val Gly

435 440 445

Lys Gln Gly Tyr Gly Lys Leu Ile Lys Arg Gln Asn Glu Asn Lys Leu

450 455 460

Gln Lys Ile Leu Lys Phe

465 470

<210> 95

<211> 471

<212> PRT

<213> 厌氧砷还原菌(Alkaliphilus oremlandii)

<400> 95

Met Lys Tyr Asp Val Ile Ile Lys Asn Ala Arg Ile Pro Gln Gly Asp

1 5 10 15

Asp Thr Met Leu Thr Asn Ile Leu Val Lys Asp Glu Lys Ile Ala Gly

20 25 30

Phe Leu Gln Asp Leu Glu Gly Ile Glu Tyr Gly Arg Glu Ile Asp Ala

35 40 45

Glu Gly Asn Leu Thr Ile Pro Gly Cys Phe Asp Ser His Thr His Phe

50 55 60

Met Asp Pro Gly Phe Thr His Arg Glu Asn Phe Leu Thr Gly Thr Ser

65 70 75 80

Ser Ala Ala Ala Gly Gly Ile Thr Thr Ile Met Asp Met Pro Cys Cys

85 90 95

Ser Lys Pro Ser Val Arg Ser Ile Pro Glu Leu Glu Ser Lys Leu Asn

100 105 110

Ala Ile Lys Asp Lys Ala Ile Ile Asp Phe Ala Phe Trp Gly Gly Val

115 120 125

Thr Gly Glu Asp Val Arg Asn Asp Asn Leu Glu His Val Lys Ala Gln

130 135 140

Ala Asp Tyr Gly Val Cys Ala Phe Lys Val Tyr Met Thr Pro Ser Val

145 150 155 160

Pro Thr Tyr Glu Arg Val Thr Asp Pro Glu Met Leu Glu Ala Phe Arg

165 170 175

Ala Val Ala Lys Thr Gly Leu Pro Val Gly Ile His Ala Glu Asn Tyr

180 185 190

Ala Met Cys Asp Tyr Tyr Val Lys Lys Phe Gln Lys Glu Gly Arg Leu

195 200 205

Asp Gly Pro Ala Trp Ala Glu Ala Arg Leu Glu Leu Ala Glu Lys Ala

210 215 220

Ala Ile Gln Leu Gly Ile Ser Phe Ala Glu Glu Thr Gly Ala Arg Leu

225 230 235 240

His Ile Val His Met Ser Thr Gly Ile Gly Ala Lys Leu Val Gly Glu

245 250 255

Ala Lys Lys Lys Gly Leu Asp Val Thr Ser Glu Thr Cys Pro His Tyr

260 265 270

Leu Thr Leu Asn Tyr Gln Asp Ala Met Thr Glu His Lys Gln Tyr Ala

275 280 285

Lys Ile Ala Pro Pro Leu Arg Thr Lys Arg Asp Asn Glu Glu Leu Trp

290 295 300

Glu Gly Leu Gln Asn Gly Ser Val Asp Phe Ile Ala Thr Asp His Ala

305 310 315 320

Pro Tyr Glu Ile Glu Thr Glu Lys Glu Ala Glu Gly Val Asn Ile Trp

325 330 335

Thr Ala Phe Pro Gly Ile Pro Gly Val Glu Thr Met Val Pro Ile Leu

340 345 350

Val Ser Glu Gly Tyr Asn Lys Gly Arg Leu Thr Leu Ser Arg Leu Val

355 360 365

Glu Val Leu Ser Thr Ser Ala Ala Lys Gln Tyr Gly Leu Tyr Pro Lys

370 375 380

Lys Gly Ala Met His Ile Gly Ser Asp Ala Asp Phe Thr Ile Ile Asp

385 390 395 400

Leu Asn Lys Glu Trp Thr Ile Asp Gln Lys Lys Thr Tyr Ser Met Ala

405 410 415

Lys Tyr Asn Pro Leu His Gly Ile Gln Leu Lys Gly Lys Pro Val Lys

420 425 430

Thr Val Val Arg Gly Thr Leu Val Tyr Asp Asp Lys Glu Gly Ile Val

435 440 445

Gly Lys Glu Gly Phe Gly Gln Phe Val Lys Arg Gln Asn Ile Gln Lys

450 455 460

Leu Asp Arg Val Ile Lys Tyr

465 470

<210> 96

<211> 471

<212> PRT

<213> Caloramator mitchellensis

<400> 96

Met Arg Tyr Asp Leu Ile Val Lys Asn Ala Leu Ile Pro His Gly Asp

1 5 10 15

Glu Thr Val Leu Thr Asn Ile Leu Val Lys Asp Glu Lys Ile Ala Gly

20 25 30

Phe Val Asp Lys Ile Glu Gly Ile Glu Ala Asp Asn Val Ile Asp Ala

35 40 45

Glu Gly His Leu Thr Leu Pro Gly Cys Ile Asp Ser His Thr His Tyr

50 55 60

Met Asp Pro Gly Phe Thr His Arg Glu Asn Phe Leu Thr Gly Thr Ser

65 70 75 80

Gly Ala Ala Val Gly Gly Val Thr Thr Ile Ile Asp Met Pro Cys Cys

85 90 95

Ser Val Pro Ser Val Arg Gly Val Ala Glu Leu His Asn Lys Val Asn

100 105 110

Ala Ile Lys Asp Lys Ala Ile Val Asp Tyr Ala Met Trp Gly Gly Val

115 120 125

Thr Gly Glu Asp Val Arg Asn Lys Asn Leu Lys Ile Val Lys Glu Gln

130 135 140

Ala Asp Glu Gly Val Val Ala Phe Lys Val Tyr Met Thr Pro Ser Val

145 150 155 160

Pro Ser Tyr Pro Arg Val Thr Asp Pro Glu Met Leu Glu Ile Phe Tyr

165 170 175

Glu Val Ser Lys Thr Gly Leu Pro Val Gly Val His Ala Glu Asn Phe

180 185 190

Ala Ile Cys Asp Tyr Met Val Gln Lys Leu Arg Glu Glu Gly Arg Met

195 200 205

Asp Gly Pro Ala Trp Ala Glu Ala Arg Met Glu Leu Ala Glu Lys Val

210 215 220

Ala Ile Gln Leu Cys Ile Ser Tyr Ala Glu Glu Thr Gly Ala Arg Phe

225 230 235 240

His Val Val His Met Ser Thr Gly Ile Gly Ala Lys Leu Val Lys Glu

245 250 255

Ala Lys Met Arg Gly Ile Asp Val Thr Ser Glu Thr Cys Pro His Tyr

260 265 270

Leu Thr Leu Asn Tyr Gln Glu His Met Thr Lys Phe Lys Gln Phe Val

275 280 285

Lys Ile Ala Pro Pro Ile Arg Thr Lys Lys Asp Asn Glu Glu Leu Trp

290 295 300

Gln Gly Leu Ile Asp Gly Ser Val Asp Phe Ile Ala Thr Asp His Ala

305 310 315 320

Pro Tyr Glu Ile Pro Thr Glu Lys Asp Ala Pro Gly Met Thr Ile Trp

325 330 335

Thr Ala Phe Pro Gly Ile Pro Gly Thr Glu Thr Met Val Pro Ile Ile

340 345 350

Val Ser Glu Gly Tyr Asn Lys Gly Arg Leu Ser Leu Ser Lys Leu Val

355 360 365

Glu Ile Leu Ser Thr Asn Ala Ala Lys His Tyr Gly Ile Tyr Pro Lys

370 375 380

Lys Gly Ala Met Met Ile Gly Ser Asp Ala Asp Phe Thr Ile Ile Asp

385 390 395 400

Leu Asn Lys Glu Trp Thr Ile Glu Lys Glu Lys Met Gln Cys Leu Asn

405 410 415

Lys Tyr Thr Pro Phe Asp Gly Met Lys Leu Lys Gly Lys Val Ala Lys

420 425 430

Thr Ile Val Arg Gly Thr Leu Val Tyr Asp Asp Glu Lys Gly Ile Val

435 440 445

Gly Lys Pro Gly Tyr Gly Gln Trp Ile Lys Arg Gln Asn Ile Gln Lys

450 455 460

Leu Glu Arg Lys Ile Lys Phe

465 470

<210> 97

<211> 480

<212> PRT

<213> 柱胞梭菌(Clostridium cylindrosporum)

<400> 97

Met Lys Phe Asp Val Ile Val Lys Asn Ala Arg Ile Pro Gln Gly His

1 5 10 15

Ala Asn Glu Thr Val Val Thr Asn Ile Leu Ile Lys Asp Glu Gln Ile

20 25 30

Ala Gly Phe Val Asn Cys Ile Asp Gly Ile Asp Ala Val Asn Val Ile

35 40 45

Asp Ala Ala Gly Asn Leu Val Leu Pro Gly Cys Ile Asp Ser His Thr

50 55 60

His Phe Met Asp Pro Gly Phe Ser His Arg Glu Thr Val Glu Ser Gly

65 70 75 80

Ser Gln Gln Ala Ala Arg Gly Gly Ile Thr Thr Val Ile Asp Met Pro

85 90 95

Cys Cys Thr Val Pro Ser Ala Arg Asn Val Asp Gln Phe Asn Trp Lys

100 105 110

Lys Glu Tyr Trp Ser Glu His Cys His Val Asp Phe Ala Leu Trp Gly

115 120 125

Gly Val Thr Gly Glu Asp Val Arg Leu Lys Gln Leu His Val Val Lys

130 135 140

Asp Gln Ala Asp Ala Gly Ala Val Ala Phe Lys Val Tyr Met Thr Pro

145 150 155 160

Ser Val Pro Thr Tyr Pro Arg Val Thr Asp Pro Glu Leu Leu Glu Ile

165 170 175

Ala Lys Ala Val Ala Glu Thr Gly Leu Ser Met Gly Val His Ala Glu

180 185 190

Asn Phe Ala Met Cys Asp Phe Tyr Val Asn Lys Leu Lys Glu Glu Gly

195 200 205

Arg Leu Asp Gly Pro Ala Trp Ala Asp Ala Arg Leu Glu Leu Ala Glu

210 215 220

Lys Val Ala Ile Glu Leu Gly Ile Ser Ile Ser Glu Ala Thr Gly Val

225 230 235 240

Arg Tyr His Val Val His Met Ser Thr Ser Ile Gly Ala Lys Leu Ile

245 250 255

Arg Gln Gly Lys Met Arg Gly Leu Asp Val Thr Ser Glu Thr Cys Pro

260 265 270

His Tyr Leu Thr Ile Asn Ala Gln Asp Ala Met Thr Glu Tyr His Ala

275 280 285

Cys Ala Lys Ile Ala Pro Pro Leu Arg Thr Lys Lys Asp Asn Ala Glu

290 295 300

Leu Trp Glu Gly Leu Ala Asn Gly Ser Val Asp Phe Ile Ala Thr Asp

305 310 315 320

His Ala Pro Tyr Asn Ile Glu Leu Glu Lys Thr Gly Arg Asp Ile Trp

325 330 335

Thr Ala Phe Pro Gly Ile Pro Gly Ile Glu Thr Met Val Glu Ile Ile

340 345 350

Val Asn Gly Phe Asn Asp Gly Lys Ile Thr Leu Glu Lys Met Val Glu

355 360 365

Ile Leu Ser Lys Asn Ala Ala Ile His Tyr Gly Leu Tyr Pro Lys Lys

370 375 380

Gly Ser Met Gln Val Gly Ser Asp Ala Asp Leu Thr Ile Ile Asp Leu

385 390 395 400

Asp Lys Glu Trp Thr Ile Asp Lys Asp Asp Lys Asp Ser Tyr Met Gln

405 410 415

Asn Lys Tyr Thr Pro Leu Gln Gly Ile Lys Leu Lys Gly Arg Val Asn

420 425 430

Lys Thr Ile Val Arg Gly His Leu Val Tyr Glu Glu Gly Thr Gly Ile

435 440 445

Ile Ser Glu Ala Gly Phe Gly Asn Phe Ile Pro Arg Gln Asn Glu Asp

450 455 460

Lys Leu Glu Arg Lys Leu Arg Phe Ile Glu Tyr Lys Pro Thr Thr Phe

465 470 475 480

<210> 98

<211> 469

<212> PRT

<213> 嗜氨菌(Ammoniphilus sp. )

<400> 98

Met Asp Leu Ile Leu Lys Asn Ala Asn Ile Pro Gln Gly Asp Arg Gln

1 5 10 15

Val Arg Thr Asn Ile Leu Val Lys Asp Glu Lys Ile Val Gly Phe Ile

20 25 30

Asp Ser Val Asp Gly Ile Gln Ala Lys Gln Val Ile Asp Met Glu Gly

35 40 45

Lys Leu Val Val Pro Gly Cys Ile Asp Pro His Thr His Phe Met Asp

50 55 60

Pro Gly Phe Glu His Arg Glu Asn Phe Ala Thr Gly Thr Met Ser Ala

65 70 75 80

Ala Ala Gly Gly Val Thr Thr Ile Ile Asp Met Pro Cys Cys Ser Lys

85 90 95

Pro Ser Val Arg Ser Glu Asp Ser Phe Tyr Ala Lys Leu Asn Pro Ile

100 105 110

Lys Glu Lys Ala Tyr Ile Asp Phe Ala Phe Trp Gly Gly Met Thr Gly

115 120 125

Glu Asp Val Arg Glu Gly Trp Leu Asp Asn Val Trp Gly Gln Ile Glu

130 135 140

Ala Gly Val Thr Ala Phe Lys Val Tyr Met Thr Pro Ser Val Pro Thr

145 150 155 160

Phe Pro Lys Val Thr Asp Ala Glu Met Leu Glu Ile Phe Tyr Lys Val

165 170 175

Ala Glu Thr Gly Leu Pro Ile Gly Ile His Ala Glu Asn Tyr Asp Ile

180 185 190

Cys Ser Phe Tyr Thr Glu Lys Leu Lys Asn Glu Gly Arg Thr Asp Gly

195 200 205

Pro Ala Trp Ala Glu Ala Arg Lys Ala Leu Ala Glu Lys Val Ala Ile

210 215 220

Glu Leu Ile Ile Ser Tyr Val Glu Glu Thr Gly Ala Arg Ala His Ile

225 230 235 240

Val His Met Ser Thr Lys Glu Gly Ala Glu Leu Ile Arg Arg Ala Lys

245 250 255

Glu Arg Gly Val Lys Ile Thr Ala Glu Thr Cys Pro His Tyr Leu Met

260 265 270

Leu Asn Ala Gln Asp Ser Met Thr Glu His Gly Thr Phe Ala Lys Ile

275 280 285

Ala Pro Pro Leu Arg Gly Lys Glu Asp Asn Glu Val His Trp Lys Ala

290 295 300

Leu Ala Asp Gly Thr Ile Asp Phe Val Gly Thr Asp His Ala Pro Tyr

305 310 315 320

Glu Ile Ala Thr Glu Lys Ala Ala Glu Asp Ser Thr Ile Trp Asn Ser

325 330 335

Phe Pro Gly Ile Pro Gly Val Glu Thr Met Val Pro Ile Leu Val Ser

340 345 350

Glu Gly Leu Asn Lys Gly Arg Leu Ser Leu Ser Arg Phe Val Glu Val

355 360 365

Thr Ser Arg Asn Ala Ala Ile His Phe Gly Leu Tyr Pro Lys Lys Gly

370 375 380

Ala Met Glu Ile Gly Ser Asp Ala Asp Phe Thr Val Ile Asp Leu Asp

385 390 395 400

Lys Glu Gly Val Ile Asp Gln Glu Lys Thr Tyr Ser Met Ala Lys Tyr

405 410 415

Asn Pro Leu His Gly Ile Lys Leu Lys Gly Met Pro Val Met Thr Ile

420 425 430

Ile Arg Gly Asn Val Val Phe Glu Asp Gly Lys Gly Ile Val Ala Glu

435 440 445

Glu Gly Phe Gly Gln Tyr Thr Lys Arg Gln Ser Pro Thr Gln Leu Gln

450 455 460

Ser Thr Leu Lys Phe

465

<210> 99

<211> 471

<212> PRT

<213> Carbydothermus pertinax

<400> 99

Met Gly Phe Asp Leu Val Leu Lys Asn Ala Lys Ile Pro Gln Gly Asp

1 5 10 15

Arg Thr Val Asp Thr His Val Leu Val Lys Asp Gly Lys Ile Ala Gly

20 25 30

Phe Thr Thr Asp Leu Thr Gly Ile Glu Val Ala Glu Val Ile Asp Ala

35 40 45

Glu Asn Asn Leu Val Leu Pro Gly Cys Ile Asp Ser His Thr His Phe

50 55 60

Asn Asp Pro Gly Phe Thr His Arg Glu Asn Phe Ser Thr Gly Thr Gln

65 70 75 80

Ala Ala Ala Ala Gly Gly Val Thr Thr Ile Ile Asp Met Pro Cys Cys

85 90 95

Ser Val Pro Ser Val Arg Ser Val Asp Asn Leu Glu Ala Lys Leu Glu

100 105 110

Val Ile Lys Asp Lys Ala Tyr Val Asp Tyr Ala Met Trp Gly Gly Ala

115 120 125

Thr Gly Glu Asp Val Arg Asn Asn Ala Leu Tyr Asn Ile Lys Glu Gln

130 135 140

Ala Asp Tyr Gly Val Val Ala Phe Lys Val Tyr Met Thr Pro Ser Val

145 150 155 160

Pro Thr Tyr Pro Pro Ala Ser Asp Ala Glu Met Leu Glu Ile Phe Arg

165 170 175

Glu Val Ala Lys Thr Gly Leu Pro Val Gly Ile His Ala Glu Asn Leu

180 185 190

Asp Leu Cys Thr Tyr His Val Asn Lys Leu Arg Lys Glu Gly Arg Met

195 200 205

Asp Gly Pro Ala Trp Ala Glu Ala Arg Leu Glu Leu Ala Glu Lys Val

210 215 220

Ala Ile Gln Met Ala Ile Ser Phe Ala Glu Glu Thr Gly Val Arg Leu

225 230 235 240

His Ile Val His Met Ser Thr Gly Ile Gly Gly Val Leu Val Gly Glu

245 250 255

Ala Lys Lys Arg Gly Ile Asp Val Thr Ala Glu Thr Cys Pro His Tyr

260 265 270

Leu Val Leu Asn Ala Tyr Glu Ser Met Thr Lys Trp Arg Gln Phe Ala

275 280 285

Lys Ile Ala Pro Pro Leu Arg Thr Thr Arg Asp Asn Glu Met Leu Trp

290 295 300

Glu Gln Leu Ala Asp Gly Arg Val Asp Phe Ile Ala Thr Asp His Ala

305 310 315 320

Pro Tyr Glu Ile Pro Thr Glu Lys Glu Ala Glu Gly Met Asp Ile Trp

325 330 335

Thr Ala Phe Pro Gly Ile Pro Gly Val Glu Thr Met Val Pro Val Ile

340 345 350

Ile Ser Glu Gly Tyr Asn Lys Gly Arg Leu Thr Leu Ser Arg Leu Val

355 360 365

Asp Val Leu Ser Arg Asn Ala Ala Ile His Tyr Gly Leu Tyr Pro Lys

370 375 380

Lys Gly Ala Leu Glu Ile Gly Ser Asp Ala Asp Phe Thr Val Ile Asp

385 390 395 400

Leu Asp Tyr Glu Trp Thr Ile Asp Lys Asp Lys Met Tyr Thr Met Ala

405 410 415

Lys Tyr Thr Pro Leu His Gly Met Lys Leu Lys Gly Lys Pro Val Lys

420 425 430

Thr Ile Val Arg Gly Lys Leu Val Tyr Asp Asp Lys Asn Gly Ile Val

435 440 445

Gly Lys Pro Gly Tyr Gly Gln Phe Val Lys Arg Gln Thr Ile Ser Lys

450 455 460

Leu Glu Arg Trp Ile Lys Phe

465 470

<210> 100

<211> 533

<212> PRT

<213> Soehngenia sp.

<400> 100

Met Met Tyr Asp Val Ile Ile Lys Asn Ala Arg Ile Pro Gln Ala Asp

1 5 10 15

Glu Thr Thr Leu Thr Asn Ile Leu Val Lys Asp Glu Lys Ile Ala Gly

20 25 30

Phe Ile Asp Asn Ile Asp Gly Ile Asp Ala Lys Glu Ile Ile Asp Ala

35 40 45

Asn Gly His Leu Thr Leu Pro Gly Cys Ile Asp Ser His Thr His Phe

50 55 60

Met Tyr Gln Gly Phe Pro His Arg Glu Asn Phe Leu Thr Gly Thr Ala

65 70 75 80

Ser Ala Ala Ala Gly Gly Val Thr Thr Ile Ile Asp Met Pro Cys Cys

85 90 95

Ser Val Pro Ser Val Arg Ser Val Glu Gln Leu Lys Leu Lys Leu Asp

100 105 110

Ala Val Ser Pro Gln Ala Leu Val Asp Phe Ala Phe Trp Gly Gly Val

115 120 125

Thr Gly Glu Asp Val Arg Asn Gly Trp Leu His Asn Val Glu Glu Gln

130 135 140

Ala Glu Tyr Gly Val Val Ala Tyr Lys Ala Tyr Met Thr Pro Ser Val

145 150 155 160

Pro Thr Tyr Pro Arg Val Asp Asp Pro Glu Met Leu Glu Ala Phe Thr

165 170 175

Glu Val Ala Lys Thr Gly Leu Pro Ile Gly Ile His Ala Glu Asn Tyr

180 185 190

Ala Met Cys Asp Tyr Tyr Val Lys Lys Leu Gln Arg Glu Gly Arg Thr

195 200 205

Asp Gly Pro Ala Trp Ala Glu Ala Arg Leu Glu Leu Ala Glu Lys Val

210 215 220

Ala Ile Glu Leu Gly Ile Ser Phe Ala Glu His Thr Gly Ala Arg Leu

225 230 235 240

His Ile Val His Met Ser Thr Gly Ile Gly Ala Lys Leu Ile Gly Glu

245 250 255

Gly Lys Lys Arg Gly Leu Asn Val Thr Ser Glu Thr Cys Pro His Tyr

260 265 270

Leu Thr Leu Asn Tyr Gln Glu Ala Met Thr Lys Tyr Gly Pro Leu Ala

275 280 285

Lys Ile Ala Pro Pro Leu Arg Thr Lys Lys Asp Asn Glu Glu Leu Trp

290 295 300

Glu Gly Leu Arg Asn Gly Ser Val Asp Phe Met Ala Thr Asp His Ala

305 310 315 320

Pro Tyr Glu Leu Glu Ser Glu Lys Phe Ser Lys Gly Met Asn Ile Trp

325 330 335

Thr Ser Phe Pro Gly Ile Pro Gly Val Glu Thr Met Val Pro Val Leu

340 345 350

Ile Ser Glu Gly Tyr Asn Lys Gly Arg Leu Ser Leu Ser Lys Leu Val

355 360 365

Glu Val Leu Ser Thr Asn Ala Ala Lys His Tyr Gly Ile Tyr Pro Lys

370 375 380

Lys Gly Ala Met Asn Ile Gly Ser Asp Ala Asp Phe Thr Ile Ile Asp

385 390 395 400

Leu Asp Lys Arg Trp Lys Val Asp Pro Lys Lys Leu Ala Ser Met Cys

405 410 415

Gly Tyr Thr Pro Leu Glu Gly Met Glu Leu Lys Gly Lys Val Ala Lys

420 425 430

Thr Ile Leu Arg Gly Thr Leu Ile Tyr Glu Asp Asn Asp Glu Ser Glu

435 440 445

Leu Ala Lys Met Thr Asp Glu Asp Leu Gln Arg Ile Val His Asn Tyr

450 455 460

Pro Glu Gly Leu Glu Glu Arg Tyr Lys Asp Ile Leu Lys Glu Tyr Pro

465 470 475 480

His Leu His Ser Lys Glu Tyr Glu Asn Ser Tyr Arg Lys Asn His Pro

485 490 495

Glu Val Ile Asp Lys His Ile Arg Gly Ile Arg Gly Ile Lys Val Lys

500 505 510

Pro Gly Phe Gly Gln Phe Val Lys Arg Gln Ser Ile Gln Val Leu Pro

515 520 525

Arg Arg Leu Thr Phe

530

<210> 101

<211> 59

<212> PRT

<213> 非解糖卟啉单胞菌(Soehngenia saccharolytica)

<400> 101

Met Ala Ile Asp Gln Ser Arg Ile Arg Asn Phe Cys Ile Ile Ala His

1 5 10 15

Ile Asp His Gly Lys Ser Thr Leu Ala Asp Arg Ile Ile Glu Ser Thr

20 25 30

Gly Thr Leu Thr Ser Arg Glu Met Gln Ala Gln Val Leu Asp Asn Met

35 40 45

Asp Leu Glu Arg Glu Arg Gly Ile Thr Ile Lys

50 55

<210> 102

<211> 491

<212> PRT

<213> Natribacillus halophilus

<400> 102

Met Asp Leu Ile Leu Lys Asn Ala His Ile Pro Gln Gly Asp Arg Gln

1 5 10 15

Val Leu Thr Asn Val Leu Val Asp Gln Gly Thr Ile Val Gly Phe Thr

20 25 30

Asn Ser Ile Glu Gly Leu Glu Ala Asp Glu Val Phe Asp Val Gln Gln

35 40 45

Lys Leu Val Val Pro Gly Cys Ile Asp Pro His Thr His Phe Met Glu

50 55 60

Pro Gly Phe Asn His Arg Asp Thr Phe Asp Ala Ala Gly Arg Ser Ala

65 70 75 80

Ala Lys Gly Gly Leu Thr Ser His Ile Asp Met Pro Cys Thr Thr Lys

85 90 95

Pro Ser Val Arg Ser Arg Glu Glu Phe Tyr Glu Lys Leu Asn Ser Ile

100 105 110

Lys Glu Lys Ala Val Ile Asp Phe Ala Phe Trp Gly Gly Met Thr Gly

115 120 125

Glu Asp Val Arg Glu Glu Lys Leu His Asn Ile Gln Pro Gln Ile Asp

130 135 140

Glu Gly Val Val Ala Phe Lys Val Tyr Met Thr Pro Ser Val Pro Thr

145 150 155 160

Phe Pro Pro Val Ser Asp Ala Glu Met Leu Glu Ile Phe Arg His Val

165 170 175

Ala Pro Thr Gly Leu Pro Ile Gly Val His Ala Glu Asn Leu Asp Ile

180 185 190

Cys Thr Phe Tyr Ser Asn Lys Leu Glu Lys Glu Gly Arg Glu Asp Pro

195 200 205

Val Ala Trp Ser Glu Ala Arg Gly Val Ile Ala Glu Lys Thr Ala Ile

210 215 220

Glu Leu Ile Leu Ser Phe Ala Glu Glu Thr Asp Ala Arg Val His Val

225 230 235 240

Val His Met Thr Ser Lys Lys Gly Val Glu Leu Val Arg Glu Ala Lys

245 250 255

Lys Arg Gly Val Lys Val Thr Ser Glu Thr Cys Pro Gln Tyr Leu Val

260 265 270

Leu Asn Ala Glu Glu Ala Met Asn Lys Trp Gly Thr Phe Ala Lys Ile

275 280 285

Ala Pro Pro Leu Arg Gly Lys Glu Asp Asn Glu Ala Phe Trp Gln Gly

290 295 300

Leu Ala Asp Gly Thr Val Asp Phe Val Gly Thr Asp His Ala Pro Tyr

305 310 315 320

Asn Ile Glu Thr Glu Lys Lys Gly Ser Thr Ile Trp Thr Ser Phe Pro

325 330 335

Gly Met Pro Gly Val Glu Gln Met Val Pro Ile Leu Val Ser Glu Gly

340 345 350

Leu Asn Lys Gly Arg Leu Ser Leu Ser Arg Phe Val Glu Val Thr Ser

355 360 365

Arg Asn Ala Ala Ile His Tyr Gly Leu Tyr Pro Lys Lys Gly Ala Met

370 375 380

Glu Leu Gly Ser Asp Ala Asp Phe Thr Val Ile Asp Leu Glu Arg Glu

385 390 395 400

Tyr Thr Ile Asp Lys Asp Asn Val Leu Thr Asp Ala Lys Tyr Thr Pro

405 410 415

Phe His Gly Met Glu Leu Lys Gly Ile Pro Val Gln Thr Ile Val Arg

420 425 430

Gly Lys Thr Val Tyr Asn Ala Asp Asp Gly Gly Ile Val Ser Glu Pro

435 440 445

Gly Tyr Gly Lys Tyr Val Glu Arg Gln Ser Ile Gln His Leu Thr Arg

450 455 460

Glu Leu Lys Tyr Glu Thr Glu Asn Thr Leu Gly Lys Pro Ile Lys Val

465 470 475 480

Gln Glu Lys Glu Lys Gln Ser Ala Gly Gly Ile

485 490

<210> 103

<211> 61

<212> PRT

<213> 堆肥宏基因组(compost metagenome)

<400> 103

Met Thr Ile Val Arg Gly Asn Val Val Tyr Glu Asp Gly Lys Gly Ile

1 5 10 15

Val Gly Gln Glu Gly Tyr Gly Gln Tyr Val Lys Arg Gln Ser Thr Gln

20 25 30

Gln Leu Asp Ala Val Ile Asn Leu Thr Lys Ser Asn Glu Ser Lys Ala

35 40 45

Val Ser Gln Ser Ser Glu Lys Lys Glu Ser Val Lys Leu

50 55 60

<210> 104

<211> 60

<212> PRT

<213> 堆肥宏基因组(compost metagenome)

<400> 104

Met His Cys Lys Pro Lys Tyr Thr Pro Phe His Gly Met Lys Leu Lys

1 5 10 15

Gly Lys Val Asp Lys Thr Ile Val Arg Gly Thr Leu Val Tyr Asp Glu

20 25 30

Ala Leu Gly Val Val Gly Lys Pro Gly His Gly Glu Tyr Val Lys Arg

35 40 45

Gln Thr Ile Ser Glu Leu Pro Arg Leu Leu Lys Tyr

50 55 60

Claims (18)

1.多肽，其包含SEQ ID NO:1所示的氨基酸序列或其功能变体，其中所述功能变体具有黄嘌呤酰胺水解酶活性。

2.如权利要求1所述的多肽，其在空间构象上具有如下定义的催化部位：

所述催化部位包含空间构象上相互靠近的、参照SEQ ID NO:1的H59、H61、K151、H186、H242和D316氨基酸残基。

3.如权利要求2所述的多肽，所述催化部位还包含二价金属离子，任选地2个二价金属离子(例如Zn²⁺和/或Mn²⁺)。

4.如权利要求1-3中任一项所述的多肽，其还包含在空间构象上具有如下定义的结合部位：

所述结合部位包含在空间构象上相互靠近的、参照SEQ ID NO:1的I288、A289、P338和G339氨基酸残基。

5.如权利要求4所述的多肽，其中所述催化部位与所述结合部位相距不大于5埃

6.如权利要求1-5中任一项所述的多肽，其中所述功能变体为SEQ ID NO:1所示的氨基酸序列的天然同工酶；

优选地，所述天然同工酶来自：坚强芽孢杆菌(Bacillus firmus)、堆肥宏基因组(compost metagenome)、Clostridium purinilyticum、高温黄色微球菌(Thermoflavimicrobium sp.)、耐盐海洋丝状菌(Marininema halotolerans)、梭菌科细菌(Clostridiaceae bacterium)、芽孢杆菌(Bacillus sp.)、类芽孢杆菌(Paenibacillussp.)、双岐高温黄色微球菌(Thermoflavimicrobium dichotomicum)、Fictibacillusenclensis、中温海洋丝状菌(Marininema mesophilum)、香蒲类芽孢杆菌(Paenibacillustyphae)、极尖组织菌(Tissierella praeacuta)、慢生型大豆根瘤菌(Bradyrhizobiumjaponicum)、产氢氨基酸杆菌(Acidaminobacter hydrogenoformans)、温铁还原细菌(Caloranaerobacter sp.)、组织菌(Tissierella sp.)、Paenibacillus donghaensis、Gottschalkia acidurici、Clostridium acidurici、强壮芽孢杆菌(Bacillus fortis)、海泥芽孢杆菌(Bacillus oceanisediminis)、Virgibacillus profundi、Anaeromicrobiumsediminis、解脂嗜热互营杆菌(Thermosyntropha lipolytica)、Alkaliphiluspeptidifermentans、米氏解硫胺素芽孢杆菌(Aneurinibacillus migulanus)、芽孢杆菌属(Bacillus bacterium)、Marinisporobacter balticus、土地芽孢杆菌(Bacillusterrae)、加利福尼亚丁达尔氏菌(Tindallia californiensis)、Romboutsialituseburensis、Paraclostridium bifermentans、Bacillus praedii、Carbydothermusislandicus、Caloramator australicus、Paraclostridium bifermentans、Tepidimicrobium xylanilyticum、Bacillus notoginsengisoli、[Clostridium]ultunense Esp、Bacillus freudenreichii、Caloramator fervidus、非解糖卟啉单胞菌(Soehngenia saccharolytica)、Thermotalea metallivorans、Ornithinibacillushalophilus、虚构芽孢杆菌(Fictibacillus sp.)、Bacillus mesonae、Tindalliamagadiensis、北拟杆菌(Paenibacillus borealis)、Paraclostridium benzoelyticum、Bacillus solani、Thermohalobacter berrensis、不动杆菌(Acinetobacter sp.)、Maledivibacter halophilus、Tissierella creatinini、Natronincola peptidivorans、Anaerovirgula multivorans、产氢羧基嗜热菌(Carbydothermus hydrogenoformans)、产乙酸互营单包菌(Sporanaerobacter acetigenes)、Proteiniborus sp.、Virgibacillusindicus、Andreesenia angusta、Paludifilum halophilum、Proteiniborusethanoligenes、Alkaliphilus metalliredigens、盐渍土假芽胞杆菌(Fictibacillussolisalsi)、Sporosarcina globispora、嗜碱菌(Alkaliphilus sp.)、厌氧砷还原菌(Alkaliphilus oremlandii)、Caloramator mitchellensis、柱胞梭菌(Clostridiumcylindrosporum)、嗜氨菌(Ammoniphilus sp.)、Carbydothermus pertinax、Soehngeniasp.和Natribacillus halophilus；

更优选地，所述天然同工酶包含SEQ ID NO:4-104中任一项所示的氨基酸序列。

7.如权利要求1-6中任一项所述的多肽，其中所述功能变体为在SEQ ID NO:1所示的氨基酸序列或其天然同工酶的基础上发生一个或多个氨基酸的插入、取代和/或缺失而产生的，可选择地，所述插入、取代和/或缺失不发生在催化部位和/或结合部位。

8.核酸分子，其编码权利要求1-7中任一项所述的多肽。

9.表达盒，其包含权利要求8所述的核酸分子。

10.表达载体，其包含权利要求8所述的核酸分子、或权利要求9所述的表达盒。

11.宿主细胞，其包含权利要求8所述的核酸分子、权利要求9所述的表达盒、或权利要求10所述的表达载体。

12.如权利要求11所述的宿主细胞，所述细胞为真核细胞或原核细胞；

优选地，所述真核细胞为酵母细胞；

优选地，所述原核细胞选自埃希氏菌属、乳酸杆菌属、双歧杆菌属、拟杆菌门和厚壁菌门；更优选地所述埃希氏菌属为大肠杆菌。

13.药物组合物或保健食品，其包含权利要求1-7中任一项所述的多肽、权利要求8所述的核酸分子、权利要求9所述的表达盒、权利要求10所述的表达载体、或者权利要求11或12所述的宿主细胞和药学上可接受的载体或赋形剂。

14.如权利要求13所述的药物组合物或保健食品，其用于预防、干预和/或治疗痛风。

15.权利要求1-7中任一项所述的多肽、权利要求8所述的核酸分子、权利要求9所述的表达盒、权利要求10所述的表达载体、权利要求11或12所述的宿主细胞、或者权利要求13或14所述的药物组合物或保健食品在降解嘌呤中的用途。

16.如权利要求15所述的用途，其中所述降解嘌呤在体外发生。

17.权利要求1-7中任一项所述的多肽、权利要求8所述的核酸分子、权利要求9所述的表达盒、权利要求10所述的表达载体、权利要求11或12所述的宿主细胞、或者权利要求13或14所述的药物组合物或保健食品在制备用于预防、干预和/或治疗痛风的药物中的用途。

18.预防、干预和/或治疗痛风的方法，包括向有需要的个体给予权利要求1-7中任一项所述的多肽、权利要求8所述的核酸分子、权利要求9所述的表达盒、权利要求10所述的表达载体、权利要求11或12所述的宿主细胞、或者权利要求13或14所述的药物组合物或保健食品。

Images