CN113171393A

CN113171393A - Traditional Chinese medicine composition for preventing influenza, preparation method and traditional Chinese medicine slow-release sachet

Info

Publication number: CN113171393A
Application number: CN202110641492.2A
Authority: CN
Inventors: 梅全喜; 杨光义; 陈琴华; 杨洋; 黄冉; 朱婧
Original assignee: Shenzhen Bao'an Pure Chinese Medicine Treatment Hospital
Current assignee: Shenzhen Bao'an Pure Chinese Medicine Treatment Hospital
Priority date: 2021-06-09
Filing date: 2021-06-09
Publication date: 2021-07-27

Abstract

The invention discloses a traditional Chinese medicine composition for preventing influenza, a preparation method and a traditional Chinese medicine slow-release sachet, belonging to the technical field of traditional Chinese medicines, wherein the traditional Chinese medicine composition comprises the following components in parts by weight: 40-60 parts of folium artemisiae argyi, 10-30 parts of rhizoma atractylodis, 10-30 parts of pogostemon cablin, 5-15 parts of cinnamon, 3-7 parts of clove, 3-7 parts of star anise, 3-7 parts of radix angelicae, 3-7 parts of mint and 1-3 parts of borneol; the traditional Chinese medicine slow-release sachet is worn with a person or placed around the person during rest, so that the fragrance can continuously act on the organism to achieve the effects of prevention and health care. The traditional Chinese medicine composition effectively acts on the prevention of influenza with different constitutions, has quick response, stable curative effect and safe medication, and is fully proved by clinical data.

Description

Traditional Chinese medicine composition for preventing influenza, preparation method and traditional Chinese medicine slow-release sachet

Technical Field

The invention relates to the technical field of traditional Chinese medicines, in particular to a traditional Chinese medicine composition for preventing influenza, a preparation method and a traditional Chinese medicine slow-release sachet.

Background

The traditional Chinese medicine sachet has a long history and is a pearl in Chinese national culture. Incense is an important means for preventing epidemic diseases in traditional Chinese medicine, and the principle of incense comes from a clothing crown therapy and an incense smelling therapy, sun Simiao is recorded in ancient books such as Stap Qianjin essentials and Li Shizhen in compendium of materia Medica. The traditional Chinese medicine sachet has a long history, and characters such as ' purple (firewood) ", ' singing ', ' incense ', ' ' (aromatic wine) are described in the carapace-bone text of the invar period. The modern times have the custom of wearing sachets and shower orchid soup. Zhou Li recorded the record of using herbs to control pests, such as Shikim fumigating and cinerary urn fumigating. The sachet has been developed all the time until the Ming Qing reaches a period of prosperity. The traditional Chinese medicine sachet which is an ancient formulation is still widely applied clinically so far, has the advantages of simplicity, convenience, experience and low cost, and is worth popularizing vigorously.

The traditional Chinese medicine belongs to the categories of epidemic diseases and epidemic febrile diseases, the traditional Chinese medicine always pays attention to the prevention of epidemic diseases, the yellow emperor's internal classic in late stage of the warring country clearly proposes ' no cure of existing diseases and no cure of diseases ', and rich prevention methods are accumulated in the long-term struggle process with the epidemic diseases, wherein the traditional Chinese medicine is mainly fumigated by aromatic medicines and has the effects of avoiding dirt, detoxifying and dissolving turbidity, and expelling pathogenic factors and preventing epidemic. At present, traditional Chinese medicine decoction and related treatment account for the majority of recovery after treatment of influenza patients. However, the traditional Chinese medicine is mainly taken orally, and the medicine is easily cause discomfort of gastrointestinal tracts due to absorption of the medicine through the digestive tracts, is lost in medicine metabolism, is large in dosage, is easy to increase the physical burden of patients, and cannot be taken for a long time.

In view of the above, the present invention is particularly proposed.

Disclosure of Invention

The invention aims to provide a traditional Chinese medicine composition for preventing influenza, a preparation method and a traditional Chinese medicine slow-release sachet, so as to solve the problems in the prior art, the traditional Chinese medicine composition has a good treatment effect on influenza and a high application value, the sachet is convenient to use, long in service life and low in cost, and the defect that traditional Chinese medicines need to be taken orally is effectively overcome.

In order to achieve the purpose, the invention provides the following scheme:

the invention provides a traditional Chinese medicine composition for preventing influenza, which comprises the following components in parts by weight:

40-60 parts of folium artemisiae argyi, 10-30 parts of rhizoma atractylodis, 10-30 parts of pogostemon cablin, 5-15 parts of cinnamon, 3-7 parts of clove, 3-7 parts of star anise, 3-7 parts of radix angelicae, 3-7 parts of mint and 1-3 parts of borneol.

Further, the coating comprises the following components in parts by weight:

45-55 parts of folium artemisiae argyi, 15-25 parts of rhizoma atractylodis, 15-25 parts of agastache rugosus, 8-12 parts of cinnamon, 4-6 parts of clove, 4-6 parts of star anise, 4-6 parts of radix angelicae, 4-6 parts of mint and 1-3 parts of borneol.

Further, the coating comprises the following components in parts by weight:

48-52 parts of folium artemisiae argyi, 18-22 parts of rhizoma atractylodis, 18-22 parts of pogostemon cablin, 9-11 parts of cinnamon, 4-5 parts of clove, 4-5 parts of star anise, 4-5 parts of radix angelicae, 4-5 parts of mint and 1-2 parts of borneol.

The invention also provides a preparation method of the traditional Chinese medicine composition for preventing influenza, which comprises the following steps:

weighing the raw materials according to the formula, taking folium artemisiae argyi, rhizoma atractylodis, pogostemon cablin, cinnamon, clove, star anise, radix angelicae and mint, removing impurities, drying, crushing, and adding borneol to obtain the traditional Chinese medicine composition for preventing influenza.

Further, the drying temperature is 50-60 ℃.

The invention also provides application of the traditional Chinese medicine composition for preventing influenza in preparation of a preparation for preventing influenza.

The invention also provides a traditional Chinese medicine slow-release sachet for preventing influenza, which comprises a shell and contents, wherein the contents comprise the traditional Chinese medicine composition for preventing influenza.

Further, the shell is made of a breathable material.

Further, the weight of the traditional Chinese medicine composition in the traditional Chinese medicine slow-release sachet is 35-40 g.

The formula of the invention is as follows:

folium artemisiae argyi: pungent and bitter with warm nature. Enter liver, spleen and kidney meridians. Channel warming, bleeding stopping, cold dispelling and pain relieving; eliminating dampness and relieving itching for external use. Can be used for treating hematemesis, epistaxis, metrorrhagia, menorrhagia, fetal leakage, lower blood, lower abdomen psychroalgia, menoxenia, and infertility due to cold womb; it can be used for treating skin pruritus. Vinegar moxa charcoal can warm meridians and stop bleeding, and is indicated for bleeding due to deficiency-cold.

Rhizoma atractylodis: pungent and bitter with warm nature. Enter spleen, stomach and liver meridians. Has the effects of eliminating dampness, strengthening spleen, dispelling pathogenic wind, dispelling cold, and improving eyesight. Can be used for treating damp obstruction of middle warmer, abdominal distention, diarrhea, edema, tinea pedis, atrophic debility cramped, rheumatalgia, wind-cold type common cold, night blindness, dim eyesight, and astringency.

Patchouli: pungent in flavor and slightly warm in nature. Enter spleen, stomach and lung meridians. Has the functions of eliminating turbid pathogen with aromatics, regulating the middle warmer and arresting vomiting, relieving exterior syndrome and relieving summer-heat. Can be used for treating damp obstruction in middle warmer, abdominal distention, emesis, summer-heat dampness exterior syndrome, early stage of damp-warm syndrome, fever, listlessness, chest distress, cold-dampness, summer-heat, abdominal pain, emesis, diarrhea, nasosinusitis, and headache.

Cinnamon: pungent and sweet in flavor and strongly hot in nature. Enter kidney, spleen, heart and liver meridians. Has the effects of tonifying fire, supporting yang, guiding fire to the origin, dispelling cold, relieving pain, warming channels and dredging collaterals. Can be used for treating sexual impotence, cold womb, cold pain of waist and knee, asthma due to kidney deficiency, floating of deficient yang, giddiness, conjunctival congestion, cold pain of heart and abdomen, vomiting and diarrhea due to deficiency cold, cold hernia, abdominal pain, dysmenorrhea, and amenorrhea.

Clove: pungent flavor and warm nature. Enter spleen, stomach, lung and kidney meridians. Has the effects of warming the middle-jiao, lowering the adverse flow of qi, tonifying the kidney and supporting yang. Can be used for treating deficiency-cold of spleen and stomach, singultus emesis, anorexia vomiting and diarrhea, psychroalgia of heart and abdomen, and sexual impotence due to kidney deficiency.

Anise: pungent flavor and warm nature. Enter liver, kidney, spleen and stomach meridians. Has the effects of warming yang, dispelling cold, regulating qi-flowing and relieving pain. Can be used for treating cold hernia with abdominal pain, lumbago due to kidney deficiency, stomach cold with emesis, and abdominal cold pain.

Radix angelicae: pungent flavor and warm nature. Enter lung, spleen and stomach meridians. Has the effects of relieving exterior syndrome, dispelling cold, dispelling pathogenic wind, relieving pain, dredging nasal orifice, eliminating dampness, relieving leukorrhagia, relieving swelling, expelling pus, dispelling pathogenic wind and relieving itching. Can be used for treating wind-cold type common cold, headache, toothache, rheumatalgia, nasosinusitis, leukorrhagia, sore, carbuncle, and toxic swelling.

Mint: pungent taste and cool nature. Enter lung and liver meridians. Has the effects of dispelling wind and heat, clearing head and eyes, relieving sore throat and promoting eruption. Can be used for treating wind-heat type common cold, headache, conjunctival congestion, sore throat, toothache, scabies, rubella, measles, and chest and hypochondrium distending pain.

The invention discloses the following technical effects:

the traditional Chinese medicine considers that the influenza belongs to the categories of 'influenza, plague and the like', the basic pathogenesis is that six exogenous pathogenic factors are carried out at any time, and the influenza invades the human body due to the fact that the qi of the acute scrofula is multiplied by the deficiency that the lung qi is not fixed at all; it is mainly caused by the attack of pathogenic wind and qi. For example, wind-cold pathogen is usually used in winter, wind-heat pathogen is used in spring, summer-heat and dampness are involved in summer, dampness is involved in plum rain, and dryness-qi is involved in autumn. The pathogenic factors are the transmission of seasonal pathogenic factors along the triple energizer and the failure of the lung to disperse and descend, which are mostly cold, dampness and toxicity. In the recipe, folium artemisiae argyi with pungent, bitter and warm natures is mainly combined with rhizoma atractylodis, the effect is strong and special, the folium artemisiae argyi is generated by endowing pure yang and healthy qi, the rhizoma atractylodis is good in nature and dryness, and in the book Ben Cao gang mu, the theory that the folium artemisiae argyi is bitter and pungent, warm in nature, cooked heat, pure yang, yang which can return to the dead yang of prolapse, twelve meridians are passed through, three yin are passed through, qi and blood are regulated, cold and dampness are dispelled, and … … can remove all diseases through all meridians. The book "materia medica justice" describes: rhizoma Atractylodis is fragrant and fragrant, and can be used for treating diseases caused by disharmony in four seasons, so it is often used for treating epidemic diseases. The two medicines are used together, so that the two medicines are the essential medicines for preventing plague and plague of the ancient people and are monarch medicines together; ageratum, clove and fennel, which are aromatic and turbid, activate spleen and open lung, can disperse dampness on the surface externally, can lower the adverse flow of lung and spleen internally, are matched with bitter, pungent and hot cinnamon, can promote and disperse qi activity blocked by cold-dampness, are particularly good at treating cough, asthma, nausea and vomiting caused by blocking lung and spleen by cold-dampness epidemic toxin, and are used as ministerial medicines together; the former herbs are all designed according to pathogenesis, the drugs are pungent and dry, the pungent and dry nature of the pungent and cool mint and borneol is less, and the mint and borneol are fresh and purgative and can also prevent the worry of heat transformation due to cold-dampness and epidemic toxin stagnation, and the drugs are adjuvant drugs; the angelica dahurica is fragrant and up-reaching, can not only disperse wind-cold-dampness evil of upper and lower channels, but also is good at entering lung to walk orifices, guiding medicine to move upwards, is matched with the rhizoma atractylodis to guide the medicines to enter lung and spleen diseases, is a guiding medicine, has the effects of dispelling cold and removing dampness, detoxifying and avoiding dirty, is suitable for the spread environment and pathogenic conditions of influenza, can emphasize viscera to strengthen healthy qi, and externally dispels cold-dampness and epidemic toxin to eliminate pathogenic qi, and is suitable for general prevention and treatment of the influenza, so the Chinese medicament is named.

Mutual reinforcement: folium Artemisiae Argyi and rhizoma Atractylodis; the following steps: rhizoma Atractylodis, herba Agastaches, flos Caryophylli, fructus Foeniculi, folium Artemisiae Argyi and cortex Cinnamomi.

Killing each other: herba Menthae, Borneolum Syntheticum, rhizoma Atractylodis, herba Agastaches, flos Caryophylli, fructus Foeniculi, folium Artemisiae Argyi, and cortex Cinnamomi.

The traditional Chinese medicine composition can effectively inhibit and kill bacteria and viruses in the air and prevent influenza through reasonable and scientific compatibility of various traditional Chinese medicine components, can enter a human body through modes of breathing or skin and the like all the time by spreading volatile components through the air to obtain the effects of removing dirt with fragrance, removing turbidity and detoxifying, is safe, has no toxic or side effect, and can be used for a long time.

The traditional Chinese medicine slow-release sachet is worn with a person or placed around the person during rest, so that the fragrance can continuously act on the organism to achieve the effects of prevention and health care. The traditional Chinese medicine composition effectively acts on the prevention of influenza with different constitutions, has quick response, stable curative effect and safe medication, and is fully proved by clinical data.

Detailed Description

Reference will now be made in detail to various exemplary embodiments of the invention, the detailed description should not be construed as limiting the invention but as a more detailed description of certain aspects, features and embodiments of the invention.

It is to be understood that the terminology used herein is for the purpose of describing particular embodiments only and is not intended to be limiting of the invention. Further, for numerical ranges in this disclosure, it is understood that each intervening value, between the upper and lower limit of that range, is also specifically disclosed. Every smaller range between any stated value or intervening value in a stated range and any other stated or intervening value in a stated range is encompassed within the invention. The upper and lower limits of these smaller ranges may independently be included or excluded in the range.

Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. Although only preferred methods and materials are described herein, any methods and materials similar or equivalent to those described herein can be used in the practice or testing of the present invention. All documents mentioned in this specification are incorporated by reference herein for the purpose of disclosing and describing the methods and/or materials associated with the documents. In case of conflict with any incorporated document, the present specification will control.

It will be apparent to those skilled in the art that various modifications and variations can be made in the specific embodiments of the present disclosure without departing from the scope or spirit of the disclosure. Other embodiments will be apparent to those skilled in the art from consideration of the specification. The description and examples are intended to be illustrative only.

As used herein, the terms "comprising," "including," "having," "containing," and the like are open-ended terms that mean including, but not limited to.

The "parts" in the present invention are all parts by mass unless otherwise specified.

Example 1

A traditional Chinese medicine composition for preventing influenza comprises the following components in parts by weight: 25g of folium artemisiae argyi, 10g of rhizoma atractylodis, 10g of pogostemon cablin, 5g of cinnamon, 2.5g of clove, 2.5g of star anise, 2.5g of angelica dahurica and 2.5g of mint.

A preparation method of a traditional Chinese medicine composition for preventing influenza comprises the following steps:

according to the formula, folium artemisiae argyi, rhizoma atractylodis, cablin potchouli herb, cinnamon, clove, star anise, radix angelicae and mint are respectively taken, impurities are removed, the mixture is dried at 55 ℃, the mixture is crushed and ground, and 1g of borneol is added.

Example 2

A traditional Chinese medicine composition for preventing influenza comprises the following components in parts by weight: 50g of folium artemisiae argyi, 20g of rhizoma atractylodis, 20g of pogostemon cablin, 10g of cinnamon, 5g of clove, 5g of star anise, 5g of radix angelicae and 5g of mint.

according to the formula, folium artemisiae argyi, rhizoma atractylodis, cablin potchouli herb, cinnamon, clove, star anise, radix angelicae and mint are respectively taken, impurities are removed, the mixture is dried at 55 ℃, the mixture is crushed and ground, and 2g of borneol is added.

Example 3

A traditional Chinese medicine composition for preventing influenza comprises the following components in parts by weight: 30g of folium artemisiae argyi, 12g of rhizoma atractylodis, 12g of pogostemon cablin, 6g of cinnamon, 3g of clove, 3g of star anise, 3g of radix angelicae and 3g of mint.

according to the formula, folium artemisiae argyi, rhizoma atractylodis, cablin potchouli herb, cinnamon, clove, star anise, radix angelicae and mint are respectively taken, impurities are removed, the mixture is dried at 55 ℃, crushed and ground, and 1.2g of borneol is added.

Example 4

A traditional Chinese medicine composition for preventing influenza comprises the following components in parts by weight: 40g of folium artemisiae argyi, 30g of rhizoma atractylodis, 10g of pogostemon cablin, 15g of cinnamon, 3g of clove, 7g of star anise, 3g of radix angelicae and 7g of mint.

according to the formula, folium artemisiae argyi, rhizoma atractylodis, cablin potchouli herb, cinnamon, clove, star anise, radix angelicae and mint are respectively taken, impurities are removed, the mixture is dried at 50 ℃, ground and ground, and 1g of borneol is added.

Example 5

A traditional Chinese medicine composition for preventing influenza comprises the following components in parts by weight: 60g of folium artemisiae argyi, 10g of rhizoma atractylodis, 30g of pogostemon cablin, 5g of cinnamon, 7g of clove, 3g of star anise, 7g of radix angelicae and 3g of mint.

according to the formula, folium artemisiae argyi, rhizoma atractylodis, cablin potchouli herb, cinnamon, clove, star anise, radix angelicae and mint are respectively taken, impurities are removed, the mixture is dried at 60 ℃, the mixture is crushed and ground, and 3g of borneol is added.

Example 6

A traditional Chinese medicine composition for preventing influenza comprises the following components in parts by weight: 45g of folium artemisiae argyi, 15g of rhizoma atractylodis, 25g of pogostemon cablin, 8g of cinnamon, 6g of clove, 4g of star anise, 6g of angelica dahurica and 4g of mint.

according to the formula, folium artemisiae argyi, rhizoma atractylodis, cablin potchouli herb, cinnamon, clove, star anise, radix angelicae and mint are respectively taken, impurities are removed, after drying at 50 ℃, crushing and grinding are carried out, and 2g of borneol is added.

Example 7

A traditional Chinese medicine composition for preventing influenza comprises the following components in parts by weight: 52g of folium artemisiae argyi, 18g of rhizoma atractylodis, 22g of pogostemon cablin, 9g of cinnamon, 5g of clove, 4g of star anise, 5g of radix angelicae and 4g of mint.

according to the formula, folium artemisiae argyi, rhizoma atractylodis, cablin potchouli herb, cinnamon, clove, star anise, radix angelicae and mint are respectively taken, impurities are removed, the mixture is dried at 60 ℃, the mixture is crushed and ground, and 1g of borneol is added.

The traditional Chinese medicine composition prepared by the embodiment can be prepared into corresponding products according to the preference of a patient, such as sachets, pouches, air fresheners, incensing agents and the like, and the effective components are volatilized into the air and directly act on local skin mucous membranes or are inhaled by the patient to play a therapeutic role.

Effect example 1

A traditional Chinese medicine slow release sachet comprises a shell and a content, wherein the content is a traditional Chinese medicine composition in any one of the embodiments. The shell is made of air-permeable material, such as non-woven fabric, cotton linen, etc.

Clinical data

1 case selection

1.1 according to the clinical diagnosis and treatment guideline (draft) for influenza made by respiratory disease of Chinese medical society, selecting students at school from 3 grades of a certain experimental primary school. 141 control groups, 69 men, 72 women, age 9-11 years; the treatment groups consisted of 147, 71 men and 76 women aged 8-11 years. The sex and age of the two groups of children are compared, and the difference has no statistical significance and is comparable.

1.2 diagnostic criteria

The disease diagnosis refers to influenza clinical diagnosis and treatment guidelines (draft) formulated by respiratory disease society of the Chinese medical society, and specifically comprises the following steps: (1) there is a history of cold exposure; (2) acute fever, aversion to cold, dry, itchy and painful throat, cough, nasal obstruction, watery nasal discharge and other symptoms or digestive tract symptoms occur; (3) checking the obvious congestion of pharynx or tonsil swelling and congestion; (4) white blood cell counts were normal or low and lymphocyte cell ratios were elevated.

1.3 inclusion criteria

The health is determined after a comprehensive examination by the medical school.

1.4 exclusion criteria

Children suffering from other chronic respiratory diseases such as influenza and allergic asthma, or who for this reason cannot be taken into or maintained in observation.

2 research methods

2.1 treatment regimens

The content of the traditional Chinese medicine slow-release sachet for preventing influenza is the traditional Chinese medicine composition in the embodiment 2 of the invention, the shell of the sachet is common cotton linen, and the traditional Chinese medicines are all purchased from medicinal material companies. Each sachet contains 35g of the Chinese medicinal composition. The medicine composition is worn between the nape and the nape in the daytime, is sniffed at variable time, can be put down on the edge of the pillow at night, starts wearing from the date of observation until the observation is finished, and is 31 days in total (the medicine inner bag is replaced about 15 days). The control group did not take any preventive measures.

2.2 Observation index

After the incense bag is put into the team, the chief and the officer of each class is responsible for checking whether the incense bag is worn according to the regulations or not and reporting the disease occurrence condition in real time. According to the clinical research guiding principle of new Chinese medicine, the scale of clinical symptoms and physical sign scores is designed, and the medical school is responsible for checking and recording the disease condition, the symptom degree and the disease course. After observation, researchers in the research institute of traditional Chinese medicine carry out summary and statistical analysis on results, and the morbidity and the average course of disease of each group, the average score of the scores of symptoms and signs, and the score of the symptoms of flu and the symptoms after the flu is suffered from are calculated.

3 results

In the clinical observation of 31d, the prevalence rate of influenza in the drug group was 6.12% (9/147), the mean course was (5.21) d, which was significantly lower than the prevalence rate of 14.89% (21/141) and the mean course (7.86) d in the control group, and the difference was statistically significant (P < 0.01). The symptoms of fever, nasal obstruction, nasal discharge and pharyngeal congestion after the onset of the influenza of the infants are obviously reduced compared with the control group (P is less than 0.05, and P is less than 0.01).

Comparative example 1

The difference from example 2 is only that clove is not added to the traditional Chinese medicine composition of comparative example 1.

Comparative example 2

The difference from example 2 is only that the borneol is replaced by the same amount of mint in the traditional Chinese medicine composition of comparative example 2.

Comparative example 3

The difference from example 2 is only that the same amount of artemisia leaves was substituted for atractylodes rhizome in the traditional Chinese medicine composition of comparative example 3.

Comparative example 4

The difference from example 2 is only that pogostemon cablin was replaced with the same amount of cinnamon in the traditional Chinese medicine composition of comparative example 4.

Comparative example 5

The difference from example 2 is only that the same amount of atractylodes rhizome was substituted for cinnamon in the traditional Chinese medicine composition of comparative example 5.

Comparative example 6

The difference from example 2 is only that the clove was replaced with the same amount of anise in the traditional Chinese medicine composition of comparative example 6.

Comparative example 7

The difference from example 2 is only that the Chinese medicinal composition of comparative example 7 is prepared by replacing the star anise with the same amount of borneol.

Comparative example 8

The difference from example 2 is only that in the traditional Chinese medicine composition of comparative example 8, the radix angelicae is replaced by the same amount of cinnamon.

Comparative example 9

The difference from example 2 is only that the mint was replaced with the same amount of patchouli in the traditional Chinese medicine composition of comparative example 9.

The Chinese medicinal compositions of the comparative examples 1 to 9 were tested again according to the above test method, and the results are shown in the following table:

	prevalence (%)	Mean course of disease (d)
			Control group	14.56	7.53
Example 2	5.84	4.95
			Comparative example 1	9.52	5.68
Comparative example 2	10.33	5.37
			Comparative example 3	8.36	5.69
Comparative example 4	10.21	5.87
			Comparative example 5	6.96	6.57
Comparative example 6	8.61	6.24
			Comparative example 7	9.85	5.94
Comparative example 8	10.92	6.92
			Comparative example 9	7.39	6.48

Animal experiment for antiviral action

1 materials of the experiment

1.1 drugs and reagents

The anti-pestilence sachet is prepared by filling the traditional Chinese medicine composition in the example 2 into sachets, wherein each sachet contains 35g of medicine powder; a nutrient broth; washing chicken red blood cells and fresh chicken blood with normal saline for 3 times; chick embryo, 9-10 days old.

1.2 Experimental viruses

Influenza A virus A/PR/8/34, influenza B virus B/Jingfang 98-76, provided by the Chinese academy of preventive medicine.

1.3 Experimental animals

ICR mice, by Guangzhou university of traditional Chinese medicine laboratory animal center. The experimental animal uses license SCXK (Su) 2002-0053.

1.4 Experimental instruments

A clean-up bench; an incubator; 24-hole micro-porous plates; self-made 20X 20cm transparent organic glass container.

1.5 culture Medium and preparation of medicine

Nutrient broth: precisely weighing 18g of nutrient broth culture medium, adding distilled water to 1000ml, heating to dissolve, subpackaging 10ml, 4.5ml, 1.6ml and 1.0ml, and autoclaving at 116 deg.C for 20min for use.

1.6 Experimental conditions

Before and after administration, mice are fed with complete pellet feed, and the mice are fed with drinking water at room temperature of 22 +/-2 ℃ and at the temperature of 55-65 ℃.2 Effect of pestilence-preventing sachet on influenza A Virus A/PR/8/34

2.1 influenza A Virus chick embryo detoxification test

Marking an air chamber on an egg candler for 9-day-old chick embryos, placing the chick embryos in a sterile chamber, respectively sterilizing the chick embryos for 2 times by using 25% ethanol and 2% iodine tincture, slightly grinding a small opening (the size of a needle point) by using a grinding wheel, adding 0.5ml of normal saline into the powder of the influenza A virus strain, uniformly mixing (dividing the mixture for 2 to 3 times), diluting the mixture to 2ml by using the normal saline after sucking the mixture out, inoculating 0.2ml of influenza A virus solution into each chick embryo after blowing and beating the chick embryo, sealing the opening by using paraffin, and placing the chick embryo in an incubator at 37 +/-0.5 ℃ for culturing for 48 hours. Taking out the chick embryo, placing the chick embryo in a refrigerator at 4 ℃ overnight, taking out the chick embryo the next day, sterilizing the chick embryo by using 75% ethanol and 2% iodine tincture, removing a paraffin wax sealing part, opening an air chamber, sucking allantoic fluid by using a sterile pipette, removing turbid allantoic fluid, collecting the allantoic fluid once for increasing toxicity, repeating the steps for 3 times, and using the collected allantoic fluid for an experiment to perform a hemagglutination test before the experiment.

2.2 hemagglutination assay

Taking a 24-hole microporous plate, adding 0.5ml of normal saline into each hole (adding 0.9ml of normal saline into the 1 st tube), adding 0.1ml of virus allantoic fluid which is subjected to 3 times of virus sterilization into the 1 st hole, uniformly mixing, adding 0.5ml of the 2 nd hole, sequentially diluting to the 8 th hole, adding no virus into the 9 th hole, and taking a blank control. Then 0.25ml of 0.5% chicken red blood cells are added into each hole, the mixture is gently mixed, and the mixture is placed at room temperature for 2 hours and observed and recorded.

Observation standard:

a layer of red blood cells is uniformly spread on the bottom of the hole in a cap shape

Substantially the same as that of +++ but with thinner edges

The + blood cells form a ring at the bottom of the tube, but have small agglomerations around it

+ the blood cells form small clusters at the bottom of the tube with smooth and regular edges

And (3) judging an experimental result: the hemagglutination titer is 2, 3, 8, 9, 10, 14 and 16 above 640, and the hemagglutination titer can be used for in vivo infection experiments.

2.3 determination of lethal dose (LD50) of influenza A virus-infected mice

Taking 56 ICR mice with weight of 13-16g and half of each mouse, randomly dividing the ICR mice into 7 groups with 8 groups, taking 2 allantoic fluids (different numbers) with blood coagulation test above 640, blowing and beating the allantoic fluids, diluting the allantoic fluids by 10 times, dripping 1 virus concentration into each group under ether light anesthesia, observing death number within 14d, calculating LD50 according to Reed Muench method, and obtaining the result that LD50 is 10^-2.11I.e. a virus concentration of 10^-2.11Half of infected mice can die at this time.

2.4 Effect of pestilence-preventing sachets on the pulmonary index of influenza A Virus A/PR8/34 infected mice

48 ICR mice were selected, weighing 13-16g, and dividing into 4 groups of 12 mice each. [1] A normal control group; [2] a model group; [3] pestilence-preventing sachet group I: fixing 2 anti-plague sachets (4g) at the top end of the container; [4] and (3) pestilence-preventing sachets II: 4 anti-pestilence sachets (8g) were fixed to the top of the container. The mice of all groups are placed in containers, 4 mice are placed in each container, after 5 days, the mice are infected by nasal drip with virus-enriched influenza A virus allantoic fluid (hemagglutination test is above 640) for 3 times under ether shallow anesthesia, each mouse has 30 microliter (20 LD50 lethal dose), before the experiment, the mice are fasted and are not forbidden for 12 hours, the mice on the day of the experiment are weighed, cervical vertebra is removed for killing, dissection is carried out, lung lesion is observed, the lung is taken for weighing, the lung index value and the lung index inhibition rate are calculated, and the difference among the groups is compared. The results are shown in Table 1.

TABLE 1 Effect of pestilence-preventing sachets on the pulmonary index of influenza A virus A/PR8/34 infected mice

P <0.05 x P <0.01 vs model groups

The experimental results show that: the first and second groups of the pestilence-preventing sachet can relieve lung diseases caused by influenza A virus and reduce lung index value. The pulmonary index inhibition rates are respectively: 29.4 percent and 28.8 percent, and the pestilence-preventing sachet has the function of relieving lung disease change caused by influenza A virus infection.

2.5 protective Effect of the pestilence-preventing sachet on the death of mice infected with influenza A Virus A/PR8/34

80 ICR mice with the weight of 13-16g and half of the weight are randomly divided into 4 groups according to the weight, and each group comprises 20 mice. [1] A normal control group; [2] a model group; [3] pestilence-preventing sachet group I: fixing 2 anti-plague sachets (4g) at the top end of the container; [4] and (3) pestilence-preventing sachets II: 4 anti-pestilence sachets (8g) were fixed to the top of the container. The mice of each group are placed in containers, 4 mice are placed in each container, after 5 days, the allantoic fluid of influenza A virus is subjected to nasal drip infection for 3 times by adding toxicity on the day of administration under ether shallow anesthesia, the mice are subjected to nasal drip infection for 30 microlitres (20 LD50 lethal dose) per mouse, the death condition of the animals within 14 days of nasal drip infection is recorded, the survival days and the survival rate are calculated, and differences among the groups are compared. The results are shown in Table 2.

TABLE 2 protective Effect of pestilence-preventing sachets on the death of influenza A virus A/PR8/34 infected mice

P <0.05 x P <0.01 vs model groups

The experimental results show that: the plague-preventing sachet II group can obviously reduce the death number of mice infected by the influenza A virus and prolong the survival days, has significant difference (P <0.05) compared with a model group, and prompts that the plague-preventing sachet has the function of resisting the influenza A virus.

Action of 3-prevention pestilence sachet on 98-76 prevention of influenza B virus B/Jingfang

3.1 influenza B Virus in chick embryo detoxification test

Marking an air chamber on an egg candler for 9-day-old chick embryos, placing the chick embryos in an aseptic chamber, respectively sterilizing the chick embryos for 2 times by using 25% ethanol and 2% iodine tincture, lightly grinding a small opening (the size of the needle tip) by using a grinding wheel, adding 0.5ml of normal saline into the powder of the influenza B virus strain, uniformly mixing (dividing the mixture for 2 to 3 times), diluting the mixture to 2ml by using the normal saline after sucking the mixture out, inoculating 0.2ml of influenza B virus venom into each chick embryo after blowing and beating, sealing the opening by using paraffin, and placing the chick embryos in an incubator at 37 +/-0.5 ℃ for culturing for 48 hours. Taking out the chick embryo, placing the chick embryo in a refrigerator at 4 ℃ overnight, taking out the chick embryo the next day, sterilizing the chick embryo by using 75% ethanol and 2% iodine tincture, removing a paraffin wax sealing part, opening an air chamber, sucking allantoic fluid by using a sterile pipette, removing turbid allantoic fluid, collecting the allantoic fluid as antitoxic fluid once, repeating the steps for 2 times, and then using the antitoxic fluid for an experiment to perform a hemagglutination test before the experiment.

3.2 hemagglutination assay

Taking a 24-hole microporous plate, adding 0.5ml of physiological saline into each hole, (adding 0.9ml of physiological saline into the 1 st tube), adding 0.1ml of virus allantoic fluid which is subjected to 3 times of virus poisoning into the 1 st hole, uniformly mixing, adding 0.5ml of the 2 nd hole, sequentially diluting to the 8 th hole, adding no virus into the 9 th hole, making a blank control, adding 0.25ml of 0.5% chicken erythrocyte into each hole, slightly uniformly mixing, standing at room temperature for 2 hours, and observing and recording.

Observation standard:

Substantially the same as that of +++ but with thinner edges

And (3) judging an experimental result: the blood coagulation titer is above 640, and the allantoic fluid of No. 1, 4, 6, 8, 11, 13, 14, 17 and 18 chick embryos can be used for in vivo infection tests.

3.3 determination of lethal dose (LD50) of influenza B virus infected mice

56 ICR mice with the weight of 13-16g and half of the weight of the ICR mice are randomly divided into 7 groups, 8 ICR mice in each group are subjected to blood coagulation test, 2 allantoic fluids (different numbers) with the blood coagulation test above 640 are taken, diluted by 10 times after being blown, 1 virus concentration is dripped into each group under ether light anesthesia, the death number in 14 days is observed, and LD50 is calculated according to a Reed Muench method. The result LD50 is 10^-1.71I.e. a virus concentration of 10^-1.71Half of infected mice can die at this time.

3.4 Effect of pestilence-preventing sachet on Lung index of influenza B virus B/Jingfang 98-76 infected mice

48 ICR mice were selected, weighing 13-16g, and dividing into 4 groups of 12 mice each. [1] A normal control group; [2] a model group; [3] pestilence-preventing sachet group I: fixing 2 anti-plague sachets (4g) at the top end of the container; [4] and (3) pestilence-preventing sachets II: 4 anti-pestilence sachets (8g) were fixed to the top of the container. The mice of all groups are placed in containers, 4 mice are placed in each container, after 5 days, the mice are infected by nasal drip with virus B influenza virus allantoic fluid (the hemagglutination test is above 640) for 3 times in ether shallow anesthesia on the day of administration, each mouse has 30 microliter (20 LD50 lethal dose), the mice are fasted before the experiment and are not forbidden for 12 hours, the mice on the day of the experiment are weighed, cervical vertebra is removed for killing, dissection is carried out, lung lesions are observed, the lung is taken out for weighing, the lung index value and the lung index inhibition rate are calculated, and the difference among the groups is compared. The results are shown in Table 3.

TABLE 3 Effect of pestilence-preventing sachets on the pulmonary index of influenza B virus B/Jingfang 98-76 infected mice

P <0.05 x P <0.01 vs model groups

The experimental results show that: the group II of pestilence-preventing sachets can relieve lung diseases caused by influenza B virus and reduce lung index value. The lung index inhibition rate is 28.7%, and the plague-preventing sachet has the function of relieving lung disease change on influenza B virus infection.

3.5 protective action of pestilence-preventing sachet on death of influenza B virus B/Jing-preventing 98-76 infected mice

80 ICR mice with the weight of 13-16g and half of the weight are randomly divided into 4 groups according to the weight, and each group comprises 20 mice. [1] A normal control group; [2] a model group; [3] pestilence-preventing sachet group I: fixing 2 anti-plague sachets (4g) at the top end of the container; [4] and (3) pestilence-preventing sachets II: 4 anti-pestilence sachets (8g) were fixed to the top of the container. The mice of each group are placed in containers, 4 mice are placed in each container, after 5 days, the allantoic fluid of influenza B virus is subjected to nasal drip infection for 3 times by adding virus (with a hemagglutination test of more than 640) on the day of administration under ether shallow anesthesia, 30 mu l of each mouse (20 LD50 lethal dose) is recorded, the death condition of the animals within 14 days of nasal drip infection is recorded, the survival days and the survival rate are calculated, and differences among the groups are compared. The results are shown in Table 4.

TABLE 4 protective action of pestilence-preventing sachet against death of influenza B virus B/Jing-preventing 98-76 infected mice

P <0.05 x P <0.01 vs model groups

The experimental results show that: the group I and the group II of the pestilence-preventing sachet have obvious protective effect on death of mice with the influenza B virus, can obviously prolong the survival days of the mice infected by the influenza B virus, has obvious difference (P <0.05) compared with a model group, and prompts that the pestilence-preventing sachet has the effect of resisting the influenza B virus.

4 small knot

The pestilence-preventing sachet can relieve lung diseases caused by influenza A virus and influenza B virus, reduce lung index value, and relieve lung diseases caused by influenza A virus and influenza B virus. And the pestilence-preventing sachet can obviously reduce the death number of mice infected by influenza A and influenza B viruses and prolong the survival days of the mice. The experiments all suggest that the anti-plague sachet has the function of resisting influenza A and influenza B viruses.

The above-described embodiments are merely illustrative of the preferred embodiments of the present invention, and do not limit the scope of the present invention, and various modifications and improvements of the technical solutions of the present invention can be made by those skilled in the art without departing from the spirit of the present invention, and the technical solutions of the present invention are within the scope of the present invention defined by the claims.

Claims

1. The traditional Chinese medicine composition for preventing influenza is characterized by comprising the following components in parts by weight:

2. The traditional Chinese medicine composition for preventing influenza according to claim 1, which comprises the following components in parts by weight:

3. The traditional Chinese medicine composition for preventing influenza according to claim 1, which comprises the following components in parts by weight:

4. A method for preparing the traditional Chinese medicine composition for preventing influenza according to any one of claims 1 to 3, which comprises the following steps:

5. The method according to claim 4, wherein the drying temperature is 50 to 60 ℃.

6. Use of the traditional Chinese medicine composition for preventing influenza according to any one of claims 1 to 3 in preparation of a preparation for preventing influenza.

7. A traditional Chinese medicine slow-release sachet for preventing influenza, which is characterized by comprising a shell and a content, wherein the content comprises the traditional Chinese medicine composition for preventing influenza according to any one of claims 1 to 3.

8. The traditional Chinese medicine slow-release sachet of claim 7, wherein the shell is made of a breathable material.

9. The traditional Chinese medicine slow-release sachet of claim 7, wherein the weight of the traditional Chinese medicine composition in the traditional Chinese medicine slow-release sachet is 35-40 g.