CN112569331A

CN112569331A - Traditional Chinese medicine composition for preventing new coronavirus, preparation method and application

Info

Publication number: CN112569331A
Application number: CN202011500278.7A
Authority: CN
Inventors: 张克华
Original assignee: Shenzhen Dingdao Health Management Co ltd
Current assignee: Shenzhen Dingdao Health Management Co ltd
Priority date: 2020-12-18
Filing date: 2020-12-18
Publication date: 2021-03-30

Abstract

The invention belongs to the field of traditional Chinese medicine compositions, and particularly discloses a traditional Chinese medicine composition for preventing new coronavirus, a preparation method and an application thereof, wherein the traditional Chinese medicine composition comprises the following traditional Chinese medicine raw materials in parts by weight: rhizoma atractylodis: 1-10 parts of cassia twig: 1-8 parts of fructus amomi: 1-9 parts of folium artemisiae argyi: 1-13 parts of clove: 1-8 parts of ligusticum wallichii: 1-7 parts of ephedra: 1-10 parts of lemongrass: 1-6 parts of asarum: 1-10 parts of bighead atractylodes rhizome: 1-10 parts of amomum cardamomum: 1-10 parts of rhizoma acori graminei: 1-10 parts of dried ginger: 1-10 parts of oldenlandia: 1-8 parts of fructus cnidii: 1-12 parts of radix angelicae: 1-15 parts of radix sileris: 1-15 parts of schizonepeta: 1-20 parts of borneol: 1-10 parts of isatis root: 1-16 parts of patchouli oil: 1-10 parts of eupatorium volatile oil: 1-15 parts. The composition has reasonable formula, and can effectively inhibit virus and bacteria activity, improve immunity, inhibit the possibility of invasion of microorganisms such as new coronavirus, influenza virus, bacteria and fungi into lung via mouth and nose, and achieve effects except drug repellency.

Description

Traditional Chinese medicine composition for preventing new coronavirus, preparation method and application

Technical Field

The invention belongs to the technical field of traditional Chinese medicine compositions, and particularly relates to a traditional Chinese medicine composition for preventing new coronavirus and a preparation method thereof.

Background

Symptoms of the new coronavirus are generally fever, hypodynamia, dry cough and gradual dyspnea, and severe patients are manifested by acute respiratory distress syndrome, septic shock, metabolic acidosis and blood coagulation dysfunction which are difficult to correct. The virus is confirmed to have a phenomenon of human transmission, and the latent period is infectious.

Because no medicine for effectively treating the novel coronavirus pneumonia exists at present, basic, supportive and symptomatic treatment is generally adopted in clinic. With the novel diagnosis and treatment scheme for coronavirus pneumonia (trial seventh edition) made by the State health and wellness Commission as a guiding principle, the current infection source is mainly a patient infected by the novel coronavirus, asymptomatic infectors can also become infection sources, and people are generally susceptible. In addition, under the background of COVID-19 aggregation onset and transmission through contact, air spray and possibly aerosol, the prevention and treatment become the key and difficult points in clinical and basic research at present. The concept of 'preventive treatment of disease' in traditional Chinese medicine is fully exerted in the prevention and treatment of the epidemic situation of the new coronary pneumonia, and the immunity of the people is improved, the proportion of susceptible people is reduced, and the symptoms of the susceptible people are relieved by adjusting the yin, the yang and the secret of the human body.

The application of the prior sachet has two thousand years of history in China, and the sachet is originally used for containing spices and is not lack of powder developed by Chinese herbal medicines with fragrance for resuscitation. As people find that the fragrance emitted by the Chinese herbal medicines is pleasant and has the effects of expelling parasites and preventing diseases. The traditional Chinese medicine sachet has an important function in human immunity. The traditional Chinese medicines in the sachet have aromatic odor, and the effects of dredging channels and collaterals, inducing resuscitation and penetrating bones are utilized to dredge viscera and channels and collaterals systems through oral and nasal inhalation and absorption of skin and channels and collaterals and acupoints, so that the whole regulation is performed on a human body, and the effects of avoiding dirt and preventing diseases are achieved. The aromatic smell given off by the Chinese herbal medicines in the sachet forms a high-concentration microenvironment around the human body, and can purify the air, kill viruses and inhibit bacteria.

Therefore, a formula of a traditional Chinese medicine preparation is developed and placed in the sachet, so that the traditional Chinese medicine preparation can be widely applied to epidemic prevention in a plurality of scenes of medical institutions, families and communities, is beneficial to cutting off a transmission path, realizes the purpose of isolating viruses and better assists in epidemic situation defense.

Disclosure of Invention

Aiming at the problems, the invention provides a traditional Chinese medicine composition for preventing new coronavirus, which not only can prevent autumn and winter influenza and allergic rhinitis caused by common virus and bacterial infection, but also can play a certain role in panic and restlessness caused by the new coronavirus and the new coronavirus. In order to achieve the purpose, the technical scheme of the invention is as follows:

a traditional Chinese medicine composition for preventing new coronavirus comprises the following traditional Chinese medicine raw materials in parts by weight: rhizoma atractylodis: 1-10 parts of cassia twig: 1-8 parts of fructus amomi: 1-9 parts of folium artemisiae argyi: 1-13 parts of clove: 1-8 parts of ligusticum wallichii: 1-7 parts of ephedra: 1-10 parts of lemongrass: 1-6 parts of asarum: 1-10 parts of bighead atractylodes rhizome: 1-10 parts of amomum cardamomum: 1-10 parts of rhizoma acori graminei: 1-10 parts of dried ginger: 1-10 parts of oldenlandia: 1-8 parts of fructus cnidii: 1-12 parts of radix angelicae: 1-15 parts of radix sileris: 1-15 parts of schizonepeta: 1-20 parts of borneol: 1-10 parts of isatis root: 1-16 parts of patchouli oil: 1-10 parts of eupatorium volatile oil: 1-15 parts.

Preferably, the traditional Chinese medicine composition for preventing the new coronavirus comprises the following traditional Chinese medicine raw materials in parts by weight: rhizoma atractylodis: 1-5 parts of cassia twig: 1-5 parts of fructus amomi: 1-6 parts of folium artemisiae argyi: 1-8 parts, 1-6 parts of clove, and ligusticum wallichii: 1-5 parts of ephedra: 1-7 parts of lemongrass: 1-4 parts of asarum: 1-6 parts of bighead atractylodes rhizome: 1-8 parts of amomum cardamomum: 1-6 parts of rhizoma acori graminei: 1-6 parts of dried ginger: 1-5 parts of oldenlandia diffusa: 1-4 parts of fructus cnidii: 1-9 parts of radix angelicae: 1-10 parts of divaricate saposhnikovia root, 1-10 parts of fineleaf schizonepeta herb: 1-15 parts of borneol: 1-7 parts of isatis root: 1-10 parts of patchouli oil: 1-8 parts of eupatorium volatile oil: 1-10 parts.

More specifically, the traditional Chinese medicine composition for preventing the new coronavirus comprises the following traditional Chinese medicine raw materials in parts by weight: rhizoma atractylodis: 2 parts of cassia twig: 2 parts of fructus amomi: 2 parts of folium artemisiae argyi: 1 part and clove: 2 parts of ligusticum wallichii: 2 parts of ephedra: 2 parts of lemongrass: 1 part of asarum: 2 parts of bighead atractylodes rhizome: 2 parts of amomum cardamomum: 2 parts of rhizoma acori graminei: 2 parts of dried ginger: 2 parts of oldenlandia: 1 part of fructus cnidii: 2 parts of radix angelicae: 2 parts of radix sileris: 2 parts of schizonepeta: 2 parts of borneol: 2 parts of isatis root: 2 parts of patchouli oil: 2 parts of eupatorium volatile oil: and 2 parts.

Preferably, the preparation method of the traditional Chinese medicine composition for treating the new coronavirus comprises the following steps: cleaning the medicinal materials, weighing rhizoma atractylodis, cassia twig, fructus amomi, folium artemisiae argyi, clove, ligusticum wallichii, ephedra, lemongrass, asarum, rhizoma atractylodis macrocephalae, amomum cardamomum, rhizoma acori graminei, rhizoma zingiberis, oldenlandia diffusa, fructus cnidii, radix angelicae, radix sileris, herba schizonepetae and radix isatidis according to the weight parts of the formula, respectively grinding the raw materials into powder with the particle size of 100-500 meshes, uniformly mixing the powder, adding patchouli oil and herba eupatorii volatile oil, uniformly grinding the powder, filling the powder into a cylinder.

The invention also provides a traditional Chinese medicine sachet for preventing and treating the new coronavirus, wherein the sachet comprises the prepared traditional Chinese medicine composition.

The preparation method of the traditional Chinese medicine sachet for preventing and treating the new coronavirus comprises the following steps:

(1) obtaining traditional Chinese medicine fine powder according to the preparation method of the traditional Chinese medicine composition;

(2) filling, compressing, sealing and packaging the traditional Chinese medicine fine powder to obtain the sachet.

The components are crushed and then uniformly mixed, so that the uniformity of the final traditional Chinese medicine fine powder can be ensured, and the drug effect can be exerted to the maximum.

In the invention, the package is that the inner bag is packaged by a small non-woven environment-friendly bag, and then the sachet is made by packing the inner bag into an outer packaging bag.

In the invention, the content of the fine powder of the traditional Chinese medicine composition packaged by the small non-woven fabric is 2.5 g/bag.

In the invention, the traditional Chinese medicine sachet for preventing and treating the new coronavirus can be carried about, placed on the edge of a pillow, hung in a vehicle, placed in an office and the like.

The invention also aims to provide application of the traditional Chinese medicine composition in medicines for treating the new coronavirus.

The invention also aims to provide application of the traditional Chinese medicine composition in medicines for treating viral diseases, infectious diseases and epidemic diseases.

Preferably, the viral disease is a viral cold or a respiratory viral infection and the infectious disease is a bacterial cold.

The raw materials in the formula of the invention have the following effects:

rhizoma atractylodis: the traditional Chinese medicines for relaxing tendons and activating collaterals have the following properties and flavors: pungent, bitter and warm; it enters spleen and stomach meridians. The efficacy is as follows: eliminating dampness, invigorating spleen, removing filth, and dispelling pathogenic wind and dampness. The main treatment is as follows: can be used for treating damp stagnation, abdominal distention, emesis, wind-cold swelling and pain, common cold due to wind-cold evil, and night blindness. The function is as follows: has antibacterial, hepatoprotective, antiarrhythmic, and blood sugar lowering effects.

Cassia twig: the pungent-warm exterior-releasing herbs have the following property-flavor effects: pungent, sweet and warm; it enters heart, lung and bladder meridians. The efficacy is as follows: induce sweating to release exterior, warm meridians and activate yang. The main treatment is as follows: can be used for treating wind-cold exterior syndrome, arthralgia due to wind-cold-dampness, arthralgia, edema, phlegm retention, thoracic obstruction, palpitation, amenorrhea due to stagnation, dysmenorrhea, and abdominal pain. The function is as follows: has antiinflammatory, antipyretic, analgesic, anticonvulsive, antiallergic, and pathogenic microorganism resisting effects.

Amomum fruit: pungent-warm-dispersing-dredging type traditional Chinese medicines, the nature, taste and channel tropism: pungent and warm; it enters spleen, stomach and kidney meridians. The efficacy is as follows: resolving dampness, promoting appetite, warming spleen, relieving diarrhea, regulating qi-flowing, etc. The main treatment is as follows: damp-turbidity obstruction in the middle, epigastric fullness without hunger, deficiency-cold of spleen and stomach, vomiting and diarrhea.

Folium artemisiae argyi: nature, flavor and meridian tropism: bitter, pungent and warm; it enters liver, spleen and kidney meridians. The efficacy is as follows: fragrant and warm powder, can ascend and descend, warm meridians and stop bleeding, dispel cold and stop pain, descend dampness and kill parasites. Bitter, dry and pungent powder can regulate qi and blood, warm meridians, expel cold-dampness and stop cold pain, so it is the key herb of gynecology. The main treatment is as follows: menoxenia, dysmenorrhea, infertility due to cold womb, threatened abortion, psychroalgia of heart and abdomen, hematemesis, epistaxis, hemoptysis, hematochezia, metrorrhagia, pregnancy hemorrhage, diarrhea, chronic dysentery, leukorrhagia, eczema, scabies, tinea, carbuncle, swelling, and hemorrhoid.

Clove: pungent and warm herbs. Nature, flavor and meridian tropism: pungent and warm; spleen, stomach, lung and kidney meridians; the efficacy is as follows: has the functions of warming the middle-jiao, lowering the adverse flow of qi, tonifying the kidney and supporting yang. The main treatment is as follows: can be used for treating deficiency-cold of spleen and stomach, singultus emesis, anorexia vomiting and diarrhea, psychroalgia of heart and abdomen, and sexual impotence due to kidney deficiency.

Ligusticum wallichii: chinese herbs entering blood circulation and removing blood stasis, property and flavor entering meridian: warming and pungent; it enters liver, gallbladder and heart meridians. The efficacy is as follows: promoting blood circulation, activating qi-flowing, dispelling pathogenic wind and relieving pain. The main treatment is as follows: can be used for treating general pain, pain of chest, thoracic obstruction, pyocutaneous disease, swelling and pain, headache, rheumatalgia, and menoxenia. The function is as follows: regulating immunity, relieving asthma, resisting tumor, inhibiting platelet aggregation, improving renal and pulmonary functions, and improving cardiovascular function.

Herba ephedrae: pungent, bitter and warm herbs, property, flavor and meridian tropism: pungent, slightly bitter and warm; it enters lung and bladder meridians. The efficacy is as follows: induce sweating and dispel cold, ventilate lung and relieve dyspnea, induce diuresis to alleviate edema. The main treatment is as follows: wind-cold exterior syndrome due to external wind-cold and closed striae, manifested as aversion to cold, fever, headache, body pain, anhidrosis, superficial and tense pulse, cough and asthma can be relieved.

And (3) lemongrass: aromatic Chinese medicinal materials. The efficacy is as follows: relieving asthma, relieving cough, sterilizing, and sterilizing. The main treatment is as follows: neuralgia, myalgia, diarrhea, common cold, fever, headache, etc.

Asarum: pungent and warm herbs. Nature, flavor and meridian tropism: pungent and warm; it enters heart, lung and kidney meridians. The efficacy is as follows: relieving exterior syndrome, dispelling cold, dispelling pathogenic wind, relieving pain, dredging orifices, warming lung, and eliminating phlegm. The main treatment is as follows: common cold due to wind-cold, headache, toothache, nasal obstruction, nasal discharge, allergic rhinitis, nasosinusitis, rheumatic arthralgia, phlegm and fluid retention, asthma and cough. The function is as follows: expelling pathogenic wind, dispelling cold, warming lung, promoting diuresis, inducing resuscitation, relieving inflammation, enhancing immunity, locally anesthetizing, improving metabolism, and resisting bacteria, and can be used for treating leukopenia caused by chemotherapy and radiotherapy for tumor patients.

White atractylodes rhizome: bitter, sweet and warm herbs. Nature, flavor and meridian tropism: bitter, sweet and warm; it enters stomach and kidney meridians. The efficacy is as follows: invigorating spleen and stomach, eliminating dampness and promoting diuresis, arresting sweating and preventing miscarriage. The main treatment is as follows: ascites due to cirrhosis, primary liver cancer, Meniere's disease, chronic lumbago, acute enteritis, leukopenia, spleen deficiency, anorexia, dyspepsia, diarrhea, edema, spontaneous perspiration, and threatened abortion.

Round cardamom fruit: pungent and warm herbs. Nature, flavor and meridian tropism: pungent and warm; it enters lung, spleen and stomach meridians. The efficacy is as follows: eliminating dampness and promoting qi circulation; warming middle energizer to arrest vomiting; promoting appetite and digestion. The main treatment is as follows: dampness obstructing qi stagnation, incoordination between spleen and stomach, abdominal distention, poor appetite, early stage of damp-warm syndrome, chest distress, no hunger, vomiting due to cold in stomach, and indigestion. The function is as follows: bacteriostasis, relieving asthma, invigorating stomach and dispelling wind.

Grassleaf sweelflag rhizome: pungent and warm herbs. Nature, flavor and meridian tropism: pungent and warm; it enters heart and stomach meridians. The efficacy is as follows: resolve dampness, stimulate appetite, induce resuscitation, eliminate phlegm, refresh mind and promote intelligence. The main treatment is as follows: epigastric fullness without hunger, vomiting and diarrhea, coma, epilepsy, amnesia, deafness, phlegm syncope, fever, coma, amnesia, qi block, deafness, vexation, stomach ache, abdominal pain, wind-cold-damp arthralgia, superficial infection, pyogenic infections, traumatic injury. The function is as follows: regulating qi, promoting blood circulation, dispelling pathogenic wind, and removing dampness.

Dried ginger: the interior-warming traditional Chinese medicines have the following properties, flavors and meridians-entering: pungent and hot herbs enter spleen, stomach, heart and lung meridians. The efficacy is as follows: warming the middle energizer to dispel cold, restoring yang to activate collaterals, warming the lung to resolve retained fluid. The main treatment is as follows: can be used for treating abdominal cold pain, emesis, diarrhea, yang exhaustion, cold limbs, slight phlegm, retained fluid, cough and asthma. The function is as follows: has effects of resisting inflammation, relieving pain, tranquilizing mind, and enhancing anoxia tolerance and blood coagulation resistance.

Herba Hedyotidis Diffusae: the heat-clearing and detoxifying traditional Chinese medicines have the following properties and flavors: sweet, bland and slightly cold, entering lung, large intestine, bladder, small intestine and stomach meridians. The functions are as follows: clearing away heat and toxic material, resolving carbuncle, dispersing pathogen accumulation, inducing diuresis and removing dampness. The main treatment is as follows: lung abscess, intestinal abscess, sore, furuncle, swelling and pain, snake venom bite. The function is as follows: it can be used for treating gastric cancer, pulmonary abscess, intestinal abscess, damp-heat-toxin, cancer and tumor.

Fructus cnidii: pungent, bitter and warm herbs. Nature, flavor and meridian tropism: pungent, bitter and warm; it enters kidney meridian. The efficacy is as follows: dry dampness and dispel wind, kill parasites and relieve itching, warm kidney and strengthen yang. The main treatment is as follows: pruritus vulvae, leukorrhagia, eczema, lumbago due to damp arthralgia, impotence due to kidney deficiency, and infertility due to cold womb.

Radix angelicae: pungent and warm herbs. Nature, flavor and meridian tropism: pungent and warm; it enters lung, spleen and stomach meridians. The efficacy is as follows: relieving exterior syndrome, dispelling cold, dispelling pathogenic wind, relieving pain, dredging nasal orifice, eliminating dampness, relieving leukorrhagia, relieving swelling, expelling pus, dispelling pathogenic wind, and relieving itching. The main treatment is as follows: wind-cold type common cold, headache, toothache, rheumatic arthralgia, nasosinusitis, leukorrhagia, sore, carbuncle and pyogenic infections.

Wind prevention: the Chinese herbs with pungent and warm properties for relieving exterior syndrome, property and flavor enter meridians: pungent, sweet and slightly warm; it enters bladder, liver and spleen meridians. The efficacy is as follows: dispel wind and relieve exterior syndrome, alleviate pain and relieve spasm. The main treatment is as follows: can be used for treating exterior syndrome due to exogenous pathogenic factor, rheumatalgia, and common cold. The function is as follows: increasing resistance and immunity, resisting bacteria, relieving inflammation, relieving pain, relieving fever, and improving sleep.

Herba schizonepetae: pungent-warm exterior-releasing herbs, property-flavor entering meridian: pungent and mild-warm; it enters lung and liver meridians. The efficacy is as follows: dispel wind, relieve exterior syndrome, promote eruption and stop bleeding. The main treatment is as follows: can be used for treating exterior syndrome due to exogenous pathogenic factors, rubella pruritus, measles without adequate eruption, pyocutaneous disease, swelling and pain, and hemorrhage. The function is as follows: has antiinflammatory, antipyretic, analgesic, hemostatic, and smooth muscle exciting effects.

Borneol: pungent and cool taste, fragrant smell, and almost insoluble in water. The main treatment is as follows: dispelling stagnated fire, promoting bone heat, treating convulsion, phlegm stagnation, pharyngitis, tongue distention, toothache, deafness, nasal obstruction, conjunctival congestion, nebula, acne, toxic invasion of internal part, parasite killing, hemorrhoid, and procreation.

Radix isatidis: bitter taste and cold nature, entering heart and stomach meridians, clearing heat and removing toxicity, cooling blood and relieving sore throat, and is good at removing toxicity, relieving sore throat and resolving masses.

Patchouli oil: pungent and mild in flavor and warm in nature, has the effects of regulating the middle warmer, dispelling dirt, eliminating dampness, eliminating turbid pathogen, regulating the middle warmer and relieving vomiting, and can be used for treating turbid dampness, fever, listlessness, chest distress, abdominal pain, vomiting and diarrhea,

herba Eupatorii volatile oil: contains cymene, nerol acetate, 5-methyl musk grass ether B, etc. The functions are as follows: relieving cough, eliminating phlegm, resisting inflammation and resisting virus. The main treatment is as follows: inhibiting influenza virus;

the main transmission path of the novel coronavirus is respiratory droplet transmission and contact transmission, the virus can be scattered on an object through air, and people directly inhale the virus-containing gas at a short distance to cause infection; or contact with contaminated hands and mucous membranes such as oral cavity, nasal cavity, eyes, etc., resulting in infection. The traditional Chinese medicine formula of the invention comprises: traditional Chinese medicines with aromatic odor, such as folium artemisiae argyi, rhizoma atractylodis and the like, have good killing effect on bacteria in the air, and can reduce the number of staphylococcus aureus, pseudomonas aeruginosa, escherichia coli, candida albicans and the like in the air; the rhizoma atractylodis has the functions of resisting inflammation, promoting gastrointestinal motility, gastric emptying and the like besides air disinfection and antibiosis; cymene, nerol acetate, 5-methyl musk grass ether B and the like contained in the eupatorium volatile oil can directly inhibit influenza viruses; the compatibility of the angelica dahurica and the divaricate saposhnikovia root has stronger antiviral activity and can effectively kill respiratory syncytial virus; patchouli oil in patchouli has broad-spectrum antiviral effect, and can kill or inhibit respiratory syncytial virus, adenovirus, coxsackie virus and A-type influenza virus; the herba schizonepetae volatile oil can resist influenza A virus; the radix Isatidis has antiviral and antibacterial effects, and can also inhibit lymphocyte count, enhance allergy defense, and improve immunity. Meanwhile, the isatis root contains a certain amount of indigoside, and after the substance enters the body, the substance can effectively promote the excretion of the body and ensure the health of the body.

Clinical tests and animal experiments show that the human respiratory mucosa can secrete more immune active substances, namely secretory immunoglobulin A (SIGA) and immunoglobulin G (IgG), through naturally inhaling volatile components of the sachet prepared by the invention through the mouth and the nose. These immunologically active substances have been found to be protective in the body's immunity and to be effective against bacteria and viruses. The SIGA is a main antibody in human mucosal immunity, and can inhibit the growth and reproduction of pathogenic bacteria on the respiratory mucosa, thereby reducing the occurrence probability of cold and respiratory tract related infectious diseases. It has also been found in some experiments that the therapeutic effect of the aerosol inhalation of a formulation containing SIgA on infantile pneumonia is superior to that of the aerosol therapy of antibiotics.

The traditional Chinese medicine preparation prepared by mutually matching the raw materials has the obvious effect of inhibiting the proliferation of new coronavirus, namely the effect of killing the new coronavirus; purifying air; has effects of killing and inhibiting bacteria, has no toxicity to human body cell (Vero-E6), can be inhaled into nostril, and made into sachet to be placed in chest and wrist, to inhibit virus and bacteria activity, improve autoimmunity, inhibit possibility of new corona virus invading lung from mouth and nose, and achieve effects of resisting virus and tranquilizing mind.

Drawings

FIGS. 1-3 are summary diagrams of the reverse transcription-qPCR results in the examples.

Detailed Description

The embodiments of the present invention for controlling a novel coronavirus will be described in detail with reference to specific examples.

The preparation method of the traditional Chinese medicine composition for treating the new coronavirus comprises the following steps: cleaning the medicinal materials, weighing rhizoma atractylodis, cassia twig, fructus amomi, folium artemisiae argyi, clove, ligusticum wallichii, ephedra, lemongrass, asarum, rhizoma atractylodis macrocephalae, amomum cardamomum, rhizoma acori graminei, rhizoma zingiberis, oldenlandia diffusa, fructus cnidii, radix angelicae, radix sileris, herba schizonepetae and radix isatidis according to the weight parts of the formula, respectively grinding the raw materials into powder with the particle size of 100-500 meshes, uniformly mixing the powder, adding patchouli oil and herba eupatorii volatile oil, uniformly grinding the powder, filling the powder into a cylinder.

Example 1:

a traditional Chinese medicine composition for preventing new coronavirus comprises the following traditional Chinese medicine raw materials in parts by weight: rhizoma atractylodis: 2 parts of cassia twig: 2 parts of fructus amomi: 2 parts of folium artemisiae argyi: 1 part and clove: 2 parts of ligusticum wallichii: 2 parts of ephedra: 2 parts of lemongrass: 1 part of asarum: 2 parts of bighead atractylodes rhizome: 2 parts of amomum cardamomum: 2 parts of rhizoma acori graminei: 2 parts of dried ginger: 2 parts of oldenlandia: 1 part of fructus cnidii: 2 parts of radix angelicae: 2 parts of radix sileris: 2 parts of schizonepeta: 2 parts of borneol: 2 parts of isatis root: 2 parts of patchouli oil: 2 parts of eupatorium volatile oil: and 2 parts.

Example 2

A traditional Chinese medicine composition for preventing new coronavirus comprises the following traditional Chinese medicine raw materials in parts by weight: rhizoma atractylodis: 3 parts of cassia twig: 2 parts of fructus amomi: 2 parts of folium artemisiae argyi: 2 parts of clove: 2 parts of ligusticum wallichii: 2 parts of ephedra: 2 parts of lemongrass: 1 part of asarum: 2 parts of bighead atractylodes rhizome: 2 parts of amomum cardamomum: 2 parts of rhizoma acori graminei: 2 parts of dried ginger: 2 parts of oldenlandia: 1 part of fructus cnidii: 2 parts of radix angelicae: 3 parts of radix sileris: 3 parts of schizonepeta: 3 parts of borneol: 2 parts of isatis root: 3 parts of patchouli oil: 3 parts of eupatorium volatile oil: and 3 parts.

Example 3

A traditional Chinese medicine composition for preventing new coronavirus comprises the following traditional Chinese medicine raw materials in parts by weight: rhizoma atractylodis: 1 part of cassia twig: 1 part and amomum fruit: 1 part of folium artemisiae argyi: 1 part and clove: 1 part of ligusticum wallichii: 1 part, ephedra: 1 part of lemongrass: 1 part of asarum: 1 part of bighead atractylodes rhizome: 1 part of amomum cardamomum: 1 part of rhizoma acori graminei: 1 part of dried ginger: 1 part, oldenlandia diffusa: 1 part of fructus cnidii: 1 part of radix angelicae: 1 part of radix sileris: 1 part of herba schizonepetae: 1 part and borneol: 1 part of radix isatidis: 1 part of patchouli oil: 1 part of eupatorium volatile oil: 1 part.

Example 4

A traditional Chinese medicine composition for preventing new coronavirus comprises the following traditional Chinese medicine raw materials in parts by weight: rhizoma atractylodis: 4 parts of cassia twig: 4 parts of fructus amomi: 5 parts of folium artemisiae argyi: 4 parts and clove: 4 parts of ligusticum wallichii: 4 parts of ephedra: 4 parts of lemongrass: 4 parts of asarum: 4 parts of bighead atractylodes rhizome: 4 parts of amomum cardamomum: 4 parts of rhizoma acori graminei: 4 parts of dried ginger: 4 parts, oldenlandia diffusa: 4 parts of fructus cnidii: 4 parts of radix angelicae: 5 parts of radix sileris: 5 parts of herba schizonepetae: 4 parts of borneol: 4 parts of isatis root: 4 parts of patchouli oil: 4 parts of eupatorium volatile oil: 4 parts.

Example 5

A traditional Chinese medicine composition for preventing new coronavirus comprises the following traditional Chinese medicine raw materials in parts by weight: rhizoma atractylodis: 5 parts of cassia twig: 5 parts of fructus amomi: 5 parts of folium artemisiae argyi: 5 parts of clove: 5 parts of ligusticum wallichii: 5 parts of ephedra: 5 parts of lemongrass: 5 parts of asarum: 5 parts of bighead atractylodes rhizome: 5 parts of amomum cardamomum: 5 parts of rhizoma acori graminei: 5 parts of dried ginger: 5 parts, oldenlandia diffusa: 5 parts of fructus cnidii: 5 parts of radix angelicae: 5 parts of radix sileris: 5 parts of herba schizonepetae: 5 parts of borneol: 5 parts of isatis root: 5 parts of patchouli oil: 5 parts of eupatorium volatile oil: 5 parts of the raw materials.

Example 6

A traditional Chinese medicine composition for preventing new coronavirus comprises the following traditional Chinese medicine raw materials in parts by weight: rhizoma atractylodis: 6 parts of cassia twig: 6 parts of fructus amomi: 6 parts of folium artemisiae argyi: 6 parts of clove: 6 parts of ligusticum wallichii: 6 parts of ephedra: 6 parts of lemongrass: 6 parts of asarum: 6 parts of bighead atractylodes rhizome: 6 parts of amomum cardamomum: 6 parts of rhizoma acori graminei: 6 parts of dried ginger: 6 parts of oldenlandia: 6 parts of fructus cnidii: 6 parts of radix angelicae: 6 parts of radix sileris: 6 parts of herba schizonepetae: 6 parts of borneol: 6 parts of radix isatidis: 6 parts of patchouli oil: 6 parts of eupatorium volatile oil: 6 parts.

Example 7

A traditional Chinese medicine composition for preventing new coronavirus comprises the following traditional Chinese medicine raw materials in parts by weight: rhizoma atractylodis: 7 parts of cassia twig: 7 parts of fructus amomi: 8 parts of folium artemisiae argyi: 7 parts of clove: 7 parts of ligusticum wallichii: 7 parts of ephedra: 7 parts of lemongrass: 7 parts of asarum: 7 parts of bighead atractylodes rhizome: 7 parts of amomum cardamomum: 8 parts of rhizoma acori graminei: 7 parts of dried ginger: 7 parts of oldenlandia: 7 parts of fructus cnidii: 7 parts of radix angelicae: 9 parts of radix sileris: 9 parts of herba schizonepetae: 10 parts of borneol: 7 parts of radix isatidis: 8 parts of patchouli oil: 7 parts of eupatorium volatile oil: 7 parts.

Example 8

A traditional Chinese medicine composition for preventing new coronavirus comprises the following traditional Chinese medicine raw materials in parts by weight: rhizoma atractylodis: 10 parts of cassia twig: 8 parts of fructus amomi: 9 parts of folium artemisiae argyi: 13 parts of clove: 8 parts of ligusticum wallichii: 7 parts of ephedra: 10 parts of lemongrass: 6 parts of asarum: 10 parts of bighead atractylodes rhizome: 10 parts of amomum cardamomum: 10 parts of rhizoma acori graminei: 10 parts of dried ginger: 7 parts of oldenlandia: 8 parts of fructus cnidii: 12 parts of radix angelicae: 15 parts of radix sileris: 15 parts of schizonepeta: 20 parts of borneol: 10 parts of isatis root: 16 parts of patchouli oil: 10 parts of eupatorium volatile oil: 15 parts.

Cleaning the traditional Chinese medicine components of the above embodiments 1-8, processing the medicinal materials, weighing the rhizoma atractylodis, the cassia twig, the fructus amomi, the folium artemisiae argyi, the clove, the ligusticum wallichii, the ephedra, the lemongrass, the asarum, the rhizoma atractylodis macrocephalae, the amomum cardamomum, the rhizoma acori graminei, the rhizoma zingiberis, the oldenlandia diffusa, the fructus cnidii, the radix angelicae, the radix sileris, the herba schizonepetae and the radix isatidis according to the weight parts; adding oleum herba Pogostemonis and herba Eupatorii volatile oil, grinding, sealing for 20 days, taking out, adding Borneolum Syntheticum, and mixing; filling, compressing, sealing and packaging the prepared traditional Chinese medicine fine powder to obtain the sachet.

Specification: each box (bag) contains 2.5g of the powder. Weight difference: the upper part and the lower part of the inner amount of each box (bag) are not more than 3 percent.

The following is a verification of the inhibitory effect of the traditional Chinese medicine formula on the new coronavirus.

Firstly, experimental materials:

Vero-E6 cell, neocoronaviruses, aqueous extract solution of the Chinese medicinal formulation of example 1

II, experimental operation:

1. preparing cells: Vero-E6 cells were plated using 24-well plates for a total of 3 plates. Plating was performed one day in advance, and the cell coverage reached 90% on the day of use (about 2.5X10 per well)⁵Individual cells).

2. The method comprises the following specific operations: collecting cell supernatant, extracting nucleic acid, performing reverse transcription-qPCR experiment, and detecting 2 targets (Orf-lab and N) of the new coronavirus and 3 targets in total, such as RNase P gene coded by human genome.

Plate 1: the aqueous extract solution of the Chinese medicinal formula in example 1 is used for testing the cytotoxicity of Vero-E6.

The aqueous extract solution was diluted 1:5, 1:25 and 1:125 times with DMEM medium containing 2% FBS, and the diluted solution lmL was added to pre-cultured Vero-E6. 3 parallel experiments were performed for each of the above concentrations of the aqueous extract solutions.

Plate 2: the virus inhibition effect of the aqueous extract solution is tested.

After diluting the aqueous extract solution with DMEM medium at 1:5, 1:25 and 1:125 times dilutions, an amount of virus suspension was added per ml of the aqueous extract solution dilution according to virus titer and cell density, such that the amount of virus per cell well reached 0.05 MOI. Adding the premixed solution of the extract aqueous solution diluent and the virus suspension into pre-cultured Vero-E6, incubating for 120 minutes at 37 ℃, discarding the premixed solution, adding the extract aqueous solution gradient dilution culture solution again, and culturing in a 37-DEG incubator. 5 parallel experiments were performed at each of the above extract stock concentrations.

Plate 3: and (3) virus proliferation experiments.

Diluting the virus suspension with DMEM medium as in the 2 nd culture plate to make the virus amount reach 0.05MOI after adding l mL of virus suspension to each well, directly adding the virus suspension to the pre-cultured Vero-E6, incubating for 2h, discarding the supernatant, and continuing culturing after supplementing DMEM medium lmL containing 2% FBS. 5 parallel experiments were performed for each of the above concentrations of the aqueous extract solutions.

In FIGS. 1-3, C1-C9 totaled 9 wells and served as Vero-E6 cell control, i.e., using precultured Vero-E6 in DMEM medium containing 2% FBS, without addition of the neocoronavirus suspension and aqueous extract solution.

Evaluation criteria: in the experiment of multiplying the new coronavirus, the Ct more than or equal to 38 of the reverse transcription-qPCR is regarded as an ineffective reaction. In addition, Ct differences for reverse transcription-qPCR were compared between sample-treated fresh coronavirus supernatants and fresh coronavirus treated groups alone.

Thirdly, experimental results and analysis:

1. the 1 st plate was an experiment for testing cytotoxicity of the aqueous extract solution, and only Ct values of RNaseP were examined. This value has a certain fluctuation in the concentration of the aqueous extract solution, but there is no effect of the dependence of the dosage of the aqueous extract solution. The aqueous extract solution itself has no significant cytotoxicity.

2. The virus proliferation experiment (plate 3) without the addition of the aqueous extract solution showed that Ct stabilized at a level of 12, which is consistent with the previous results. In the virus proliferation experiment in which the supernatant was treated with the aqueous extract solution, the difference in Ct was at least 14 as compared with the result of the aforementioned 3 rd culture plate, and the difference had a significant dose-dependent effect on the aqueous extract solution.

RNaseP, which is a housekeeping gene encoded by the human genome, must express the gene in active cells, and thus the Ct value of reverse transcription-qPCR of RNase P, the presence or absence thereof, can be used as an index of whether cultured cells are active, and the size thereof can be used as an index of the proportion of active cells in cultured cells. The Ct data indicate that the aqueous extract solution does not have significant inhibitory effect on cell growth, i.e., the aqueous extract solution does not have significant toxicity to cells.

Fourthly, conclusion:

based on the above research data, the aqueous extract solution has no obvious toxicity to Vero-E6 cells. Meanwhile, the aqueous extract solution has a remarkable effect of inhibiting the proliferation of the new coronavirus, namely the aqueous extract solution has the effect of killing the new coronavirus.

Application examples

500 persons were recruited and the sachets prepared in examples 1-8 were worn on these volunteers for 2 months, and the subjects did not show symptoms of seasonal influenza such as cold, fever, and limp limbs and symptoms of new coronavirus infection.

It is to be understood that while the present invention has been described in conjunction with the preferred embodiments thereof, it is not intended to limit the invention to those embodiments. It will be apparent to those skilled in the art from this disclosure that many changes and modifications can be made, or equivalents modified, in the embodiments of the invention without departing from the scope of the invention. Therefore, any simple modification, equivalent change and modification made to the above embodiments according to the technical essence of the present invention are still within the scope of the protection of the technical solution of the present invention, unless the contents of the technical solution of the present invention are departed.

Claims

1. A traditional Chinese medicine composition for preventing new coronavirus is characterized in that: the traditional Chinese medicine composition comprises the following traditional Chinese medicine raw materials in parts by weight: rhizoma atractylodis: 1-10 parts of cassia twig: 1-8 parts of fructus amomi: 1-9 parts of folium artemisiae argyi: 1-13 parts of clove: 1-8 parts of ligusticum wallichii: 1-7 parts of ephedra: 1-10 parts of lemongrass: 1-6 parts of asarum: 1-10 parts of bighead atractylodes rhizome: 1-10 parts of amomum cardamomum: 1-10 parts of rhizoma acori graminei: 1-10 parts of dried ginger: 1-10 parts of oldenlandia: 1-8 parts of fructus cnidii: 1-12 parts of radix angelicae: 1-15 parts of radix sileris: 1-15 parts of schizonepeta: 1-20 parts of borneol: 1-10 parts of isatis root: 1-16 parts of patchouli oil: 1-10 parts of eupatorium volatile oil: 1-15 parts.

2. The traditional Chinese medicine composition for preventing new coronavirus according to claim 1, which is characterized in that: the traditional Chinese medicine composition comprises the following traditional Chinese medicine raw materials in parts by weight: rhizoma atractylodis: 1-5 parts of cassia twig: 1-5 parts of fructus amomi: 1-6 parts of folium artemisiae argyi: 1-8 parts of clove: 1-6 parts of ligusticum wallichii: 1-5 parts of ephedra: 1-7 parts of lemongrass: 1-4 parts of asarum: 1-6 parts of bighead atractylodes rhizome: 1-8 parts of amomum cardamomum: 1-6 parts of rhizoma acori graminei: 1-6 parts of dried ginger: 1-5 parts of oldenlandia diffusa: 1-4 parts of fructus cnidii: 1-9 parts of radix angelicae: 1-10 parts of radix sileris: 1-10 parts of schizonepeta: 1-15 parts of borneol: 1-7 parts of isatis root: 1-10 parts of patchouli oil: 1-8 parts of eupatorium volatile oil: 1-10 parts.

3. The traditional Chinese medicine composition for preventing new coronavirus according to claim 2, which is characterized in that: the traditional Chinese medicine composition comprises the following traditional Chinese medicine raw materials in parts by weight: rhizoma atractylodis: 2 parts of cassia twig: 2 parts of fructus amomi: 2 parts of folium artemisiae argyi: 1 part and clove: 2 parts of ligusticum wallichii: 2 parts of ephedra: 2 parts of lemongrass: 1 part of asarum: 2 parts of bighead atractylodes rhizome: 2 parts of amomum cardamomum: 2 parts of rhizoma acori graminei: 2 parts of dried ginger: 2 parts of oldenlandia: 1 part of fructus cnidii: 2 parts of radix angelicae: 2 parts of radix sileris: 2 parts of schizonepeta: 2 parts of borneol: 2 parts of isatis root: 2 parts of patchouli oil: 2 parts of eupatorium volatile oil: and 2 parts.

4. The method for preparing the traditional Chinese medicine composition for treating the neocoronavirus according to any one of claims 1 to 3, which is characterized by comprising the following steps: cleaning the medicinal materials, weighing rhizoma atractylodis, cassia twig, fructus amomi, folium artemisiae argyi, clove, ligusticum wallichii, ephedra, lemongrass, asarum, bighead atractylodes rhizome, amomum cardamomum, rhizoma acori graminei, rhizoma zingiberis, oldenlandia diffusa, fructus cnidii, radix angelicae, radix sileris, herba schizonepetae and radix isatidis according to the weight part formula, respectively grinding the raw materials into powder with the particle size of 100-500 meshes, and uniformly mixing the powder; adding oleum herba Pogostemonis and herba Eupatorii volatile oil, grinding, sealing for 10-30 days, taking out, adding Borneolum Syntheticum, and mixing.

5. The use of the Chinese medicinal composition of any one of claims 1 to 4 in the preparation of a medicament for treating a new coronavirus.

6. The use of the Chinese medicinal composition of any one of claims 1 to 4 in the preparation of medicaments for treating viral diseases, infectious diseases and epidemic diseases.

7. Use according to claim 6, characterized in that: the viral disease is viral cold or respiratory virus infection, and the infectious disease is bacterial cold.

8. A traditional Chinese medicine sachet for preventing and treating new coronavirus is characterized in that: comprises the traditional Chinese medicine composition of claims 1-3.

9. The preparation method of the traditional Chinese medicine sachet for preventing and treating the neocoronaviruses as claimed in claim 8, which is characterized in that: the method comprises the following steps:

(1) obtaining fine powder of a Chinese medicinal material according to the method for preparing a Chinese medicinal composition of claim 4;