CN113164915A - 固相螯合剂材料，其制备方法及其用于纯化蛋白质的用途 - Google Patents

固相螯合剂材料，其制备方法及其用于纯化蛋白质的用途 Download PDF

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CN113164915A
CN113164915A CN201980078337.5A CN201980078337A CN113164915A CN 113164915 A CN113164915 A CN 113164915A CN 201980078337 A CN201980078337 A CN 201980078337A CN 113164915 A CN113164915 A CN 113164915A
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R·法比斯
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Coobe Biotechnology Co ltd
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Coobe Biotechnology Co ltd
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    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J45/00—Ion-exchange in which a complex or a chelate is formed; Use of material as complex or chelate forming ion-exchangers; Treatment of material for improving the complex or chelate forming ion-exchange properties
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/38—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving specific interaction not covered by one or more of groups B01D15/265 and B01D15/30 - B01D15/36, e.g. affinity, ligand exchange or chiral chromatography
    • B01D15/3804—Affinity chromatography
    • B01D15/3828—Ligand exchange chromatography, e.g. complexation, chelation or metal interaction chromatography
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/281—Sorbents specially adapted for preparative, analytical or investigative chromatography
    • B01J20/286—Phases chemically bonded to a substrate, e.g. to silica or to polymers
    • B01J20/288—Polar phases
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/30—Processes for preparing, regenerating, or reactivating
    • B01J20/32—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating
    • B01J20/3202—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating characterised by the carrier, support or substrate used for impregnation or coating
    • B01J20/3204—Inorganic carriers, supports or substrates
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/30—Processes for preparing, regenerating, or reactivating
    • B01J20/32—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating
    • B01J20/3202—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating characterised by the carrier, support or substrate used for impregnation or coating
    • B01J20/3206—Organic carriers, supports or substrates
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/30—Processes for preparing, regenerating, or reactivating
    • B01J20/32—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating
    • B01J20/3202—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating characterised by the carrier, support or substrate used for impregnation or coating
    • B01J20/3206—Organic carriers, supports or substrates
    • B01J20/3208—Polymeric carriers, supports or substrates
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/30—Processes for preparing, regenerating, or reactivating
    • B01J20/32—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating
    • B01J20/3214—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating characterised by the method for obtaining this coating or impregnating
    • B01J20/3217—Resulting in a chemical bond between the coating or impregnating layer and the carrier, support or substrate, e.g. a covalent bond
    • B01J20/3219—Resulting in a chemical bond between the coating or impregnating layer and the carrier, support or substrate, e.g. a covalent bond involving a particular spacer or linking group, e.g. for attaching an active group
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/30—Processes for preparing, regenerating, or reactivating
    • B01J20/32—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating
    • B01J20/3231—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating characterised by the coating or impregnating layer
    • B01J20/3242—Layers with a functional group, e.g. an affinity material, a ligand, a reactant or a complexing group
    • B01J20/3244—Non-macromolecular compounds
    • B01J20/3246—Non-macromolecular compounds having a well defined chemical structure
    • B01J20/3248—Non-macromolecular compounds having a well defined chemical structure the functional group or the linking, spacer or anchoring group as a whole comprising at least one type of heteroatom selected from a nitrogen, oxygen or sulfur, these atoms not being part of the carrier as such
    • B01J20/3251—Non-macromolecular compounds having a well defined chemical structure the functional group or the linking, spacer or anchoring group as a whole comprising at least one type of heteroatom selected from a nitrogen, oxygen or sulfur, these atoms not being part of the carrier as such comprising at least two different types of heteroatoms selected from nitrogen, oxygen or sulphur
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/30—Processes for preparing, regenerating, or reactivating
    • B01J20/32—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating
    • B01J20/3231—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating characterised by the coating or impregnating layer
    • B01J20/3242—Layers with a functional group, e.g. an affinity material, a ligand, a reactant or a complexing group
    • B01J20/3244—Non-macromolecular compounds
    • B01J20/3265—Non-macromolecular compounds with an organic functional group containing a metal, e.g. a metal affinity ligand
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/22—Affinity chromatography or related techniques based upon selective absorption processes

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Molecular Biology (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Inorganic Chemistry (AREA)
  • Peptides Or Proteins (AREA)
  • Treatment Of Liquids With Adsorbents In General (AREA)
  • Solid-Sorbent Or Filter-Aiding Compositions (AREA)
CN201980078337.5A 2018-11-28 2019-11-22 固相螯合剂材料，其制备方法及其用于纯化蛋白质的用途 Pending CN113164915A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201862772206P 2018-11-28 2018-11-28
US62/772,206 2018-11-28
PCT/EP2019/082224 WO2020109162A1 (en) 2018-11-28 2019-11-22 Solid-phase chelator material, method for producing thereof and use thereof for the purification of proteins

Publications (1)

Publication Number Publication Date
CN113164915A true CN113164915A (zh) 2021-07-23

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CN201980078337.5A Pending CN113164915A (zh) 2018-11-28 2019-11-22 固相螯合剂材料，其制备方法及其用于纯化蛋白质的用途

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US (1) US12403461B2 (https=)
EP (1) EP3887033B1 (https=)
JP (1) JP7544704B2 (https=)
CN (1) CN113164915A (https=)
CA (1) CA3121103C (https=)
DK (1) DK3887033T3 (https=)
WO (1) WO2020109162A1 (https=)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN117169393A (zh) * 2023-11-03 2023-12-05 杭州湃肽生化科技有限公司 一种植物组织中环肽的检测方法
CN117339580A (zh) * 2023-12-05 2024-01-05 凯莱英生命科学技术(天津)有限公司 螯合载体、其制备方法及应用

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WO2023044368A1 (en) * 2021-09-17 2023-03-23 Doublerainbow Biosciences Inc. Method for producing glycosylated therapeutics by using an immobilized enzyme preparation
CN115350682A (zh) * 2022-08-16 2022-11-18 青海师范大学 一种富含羧基的凹凸棒复合材料及其制备方法和应用
CA3267735A1 (en) 2022-09-15 2024-03-21 Cube Biotech Gmbh IN VITRO DIAGNOSTIC METHOD FOR DETECTING THE PRESENCE OF A TARGET USING STABILIZED MEMBRANE PROTEINS
EP4621407A1 (en) * 2022-12-13 2025-09-24 Kazusa DNA Research Institute Method for preparing peptide-containing sample
EP4417697A1 (en) 2023-02-17 2024-08-21 Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V. Modular conjugation system
CN116063733A (zh) * 2023-02-20 2023-05-05 苏州赛分科技股份有限公司 一种基于ted的高耐受金属螯合试剂亲和填料及其制备方法和应用
WO2025125266A1 (en) 2023-12-11 2025-06-19 Cube Biotech Gmbh Tablets containing fuctionalized agarose particles, method for producing them and use of the tablets
CN118888738B (zh) * 2024-09-30 2024-11-29 溧阳中科海钠科技有限责任公司 一种锡碳复合负极材料及其制备方法与应用

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CN86103488A (zh) * 1985-04-26 1987-05-27 奥托·A·冈索 一种金属螯合蛋白质配合物的形成方法
US5281704A (en) * 1989-10-23 1994-01-25 Salutar, Inc. Polychelant compounds
CN1139882A (zh) * 1994-03-04 1997-01-08 耐克麦德瑟鲁塔公司 多螯合剂
US20050208118A1 (en) * 2004-03-18 2005-09-22 Takemoto Arnold C Preparations of encapsulated bioavailable chelating agents for detoxifying humans and animals
CN1898265A (zh) * 2003-12-23 2007-01-17 通用电气健康护理生物科学股份公司 免疫球蛋白的纯化方法
US20080038750A1 (en) * 2004-08-05 2008-02-14 Jacob Piehler Multivalent Chelators for Modifying and Organizing of Target Molecules
US20120068113A1 (en) * 2009-01-26 2012-03-22 Innospec Limited Chelating agents and methods relating thereto
CN105418860A (zh) * 2014-09-15 2016-03-23 赢创德固赛有限公司 用作超吸收剂制造中的加工助剂的氨基多羧酸
WO2017046625A1 (en) * 2015-06-25 2017-03-23 Cube Biotech Gmbh New chelators for affinity purification of recombinant proteins

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CA1304886C (en) 1986-07-10 1992-07-07 Heinz Dobeli Metal chelate resins
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US6623655B1 (en) 2000-04-24 2003-09-23 Sigma-Aldrich Co. Metal chelating compositions
JP2008508333A (ja) 2004-08-05 2008-03-21 ヨハン ウォルフガング ゲーテ−ウニベルジテート フランクフルト アム マイン 標的分子の改変および組織化のための多価キレーター
WO2009008802A1 (en) 2007-07-09 2009-01-15 Ge Healthcare Bio-Sciences Ab Method for preparation of a biomolecule adsorbent
EP2022561A1 (en) 2007-08-06 2009-02-11 Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V. Immobilisation of chelating groups for immobilised metal ion chromatography (IMAC)
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US20050208118A1 (en) * 2004-03-18 2005-09-22 Takemoto Arnold C Preparations of encapsulated bioavailable chelating agents for detoxifying humans and animals
US20080038750A1 (en) * 2004-08-05 2008-02-14 Jacob Piehler Multivalent Chelators for Modifying and Organizing of Target Molecules
US20120068113A1 (en) * 2009-01-26 2012-03-22 Innospec Limited Chelating agents and methods relating thereto
CN105418860A (zh) * 2014-09-15 2016-03-23 赢创德固赛有限公司 用作超吸收剂制造中的加工助剂的氨基多羧酸
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Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN117169393A (zh) * 2023-11-03 2023-12-05 杭州湃肽生化科技有限公司 一种植物组织中环肽的检测方法
CN117169393B (zh) * 2023-11-03 2024-03-19 杭州湃肽生化科技有限公司 一种植物组织中环肽的检测方法
CN117339580A (zh) * 2023-12-05 2024-01-05 凯莱英生命科学技术(天津)有限公司 螯合载体、其制备方法及应用
CN117339580B (zh) * 2023-12-05 2024-04-02 凯莱英生命科学技术(天津)有限公司 螯合载体、其制备方法及应用

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US12403461B2 (en) 2025-09-02
EP3887033B1 (en) 2024-07-24
JP2022509239A (ja) 2022-01-20
JP7544704B2 (ja) 2024-09-03
WO2020109162A1 (en) 2020-06-04
CA3121103A1 (en) 2020-06-04
EP3887033A1 (en) 2021-10-06
US20220023851A1 (en) 2022-01-27
DK3887033T3 (da) 2024-08-12
CA3121103C (en) 2023-10-10

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Application publication date: 20210723