CA3121103C - Solid-phase chelator material, method for producing thereof and use thereof for the purification of proteins - Google Patents

Solid-phase chelator material, method for producing thereof and use thereof for the purification of proteins Download PDF

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Publication number
CA3121103C
CA3121103C CA3121103A CA3121103A CA3121103C CA 3121103 C CA3121103 C CA 3121103C CA 3121103 A CA3121103 A CA 3121103A CA 3121103 A CA3121103 A CA 3121103A CA 3121103 C CA3121103 C CA 3121103C
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Prior art keywords
solid
phase
groups
apa
group
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CA3121103A
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English (en)
French (fr)
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CA3121103A1 (en
Inventor
Roland Fabis
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Cube Biotech GmbH
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Cube Biotech GmbH
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Publication of CA3121103A1 publication Critical patent/CA3121103A1/en
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Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J45/00—Ion-exchange in which a complex or a chelate is formed; Use of material as complex or chelate forming ion-exchangers; Treatment of material for improving the complex or chelate forming ion-exchange properties
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/38—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving specific interaction not covered by one or more of groups B01D15/265 and B01D15/30 - B01D15/36, e.g. affinity, ligand exchange or chiral chromatography
    • B01D15/3804—Affinity chromatography
    • B01D15/3828—Ligand exchange chromatography, e.g. complexation, chelation or metal interaction chromatography
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/281—Sorbents specially adapted for preparative, analytical or investigative chromatography
    • B01J20/286—Phases chemically bonded to a substrate, e.g. to silica or to polymers
    • B01J20/288—Polar phases
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/30—Processes for preparing, regenerating, or reactivating
    • B01J20/32—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating
    • B01J20/3202—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating characterised by the carrier, support or substrate used for impregnation or coating
    • B01J20/3204—Inorganic carriers, supports or substrates
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/30—Processes for preparing, regenerating, or reactivating
    • B01J20/32—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating
    • B01J20/3202—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating characterised by the carrier, support or substrate used for impregnation or coating
    • B01J20/3206—Organic carriers, supports or substrates
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/30—Processes for preparing, regenerating, or reactivating
    • B01J20/32—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating
    • B01J20/3202—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating characterised by the carrier, support or substrate used for impregnation or coating
    • B01J20/3206—Organic carriers, supports or substrates
    • B01J20/3208—Polymeric carriers, supports or substrates
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/30—Processes for preparing, regenerating, or reactivating
    • B01J20/32—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating
    • B01J20/3214—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating characterised by the method for obtaining this coating or impregnating
    • B01J20/3217—Resulting in a chemical bond between the coating or impregnating layer and the carrier, support or substrate, e.g. a covalent bond
    • B01J20/3219—Resulting in a chemical bond between the coating or impregnating layer and the carrier, support or substrate, e.g. a covalent bond involving a particular spacer or linking group, e.g. for attaching an active group
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/30—Processes for preparing, regenerating, or reactivating
    • B01J20/32—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating
    • B01J20/3231—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating characterised by the coating or impregnating layer
    • B01J20/3242—Layers with a functional group, e.g. an affinity material, a ligand, a reactant or a complexing group
    • B01J20/3244—Non-macromolecular compounds
    • B01J20/3246—Non-macromolecular compounds having a well defined chemical structure
    • B01J20/3248—Non-macromolecular compounds having a well defined chemical structure the functional group or the linking, spacer or anchoring group as a whole comprising at least one type of heteroatom selected from a nitrogen, oxygen or sulfur, these atoms not being part of the carrier as such
    • B01J20/3251—Non-macromolecular compounds having a well defined chemical structure the functional group or the linking, spacer or anchoring group as a whole comprising at least one type of heteroatom selected from a nitrogen, oxygen or sulfur, these atoms not being part of the carrier as such comprising at least two different types of heteroatoms selected from nitrogen, oxygen or sulphur
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/30—Processes for preparing, regenerating, or reactivating
    • B01J20/32—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating
    • B01J20/3231—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating characterised by the coating or impregnating layer
    • B01J20/3242—Layers with a functional group, e.g. an affinity material, a ligand, a reactant or a complexing group
    • B01J20/3244—Non-macromolecular compounds
    • B01J20/3265—Non-macromolecular compounds with an organic functional group containing a metal, e.g. a metal affinity ligand
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/22—Affinity chromatography or related techniques based upon selective absorption processes

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Molecular Biology (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Inorganic Chemistry (AREA)
  • Peptides Or Proteins (AREA)
  • Treatment Of Liquids With Adsorbents In General (AREA)
  • Solid-Sorbent Or Filter-Aiding Compositions (AREA)
CA3121103A 2018-11-28 2019-11-22 Solid-phase chelator material, method for producing thereof and use thereof for the purification of proteins Active CA3121103C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201862772206P 2018-11-28 2018-11-28
US62/772,206 2018-11-28
PCT/EP2019/082224 WO2020109162A1 (en) 2018-11-28 2019-11-22 Solid-phase chelator material, method for producing thereof and use thereof for the purification of proteins

Publications (2)

Publication Number Publication Date
CA3121103A1 CA3121103A1 (en) 2020-06-04
CA3121103C true CA3121103C (en) 2023-10-10

Family

ID=68887375

Family Applications (1)

Application Number Title Priority Date Filing Date
CA3121103A Active CA3121103C (en) 2018-11-28 2019-11-22 Solid-phase chelator material, method for producing thereof and use thereof for the purification of proteins

Country Status (7)

Country Link
US (1) US12403461B2 (https=)
EP (1) EP3887033B1 (https=)
JP (1) JP7544704B2 (https=)
CN (1) CN113164915A (https=)
CA (1) CA3121103C (https=)
DK (1) DK3887033T3 (https=)
WO (1) WO2020109162A1 (https=)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2023044368A1 (en) * 2021-09-17 2023-03-23 Doublerainbow Biosciences Inc. Method for producing glycosylated therapeutics by using an immobilized enzyme preparation
CN115350682A (zh) * 2022-08-16 2022-11-18 青海师范大学 一种富含羧基的凹凸棒复合材料及其制备方法和应用
CA3267735A1 (en) 2022-09-15 2024-03-21 Cube Biotech Gmbh IN VITRO DIAGNOSTIC METHOD FOR DETECTING THE PRESENCE OF A TARGET USING STABILIZED MEMBRANE PROTEINS
EP4621407A1 (en) * 2022-12-13 2025-09-24 Kazusa DNA Research Institute Method for preparing peptide-containing sample
EP4417697A1 (en) 2023-02-17 2024-08-21 Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V. Modular conjugation system
CN116063733A (zh) * 2023-02-20 2023-05-05 苏州赛分科技股份有限公司 一种基于ted的高耐受金属螯合试剂亲和填料及其制备方法和应用
CN117169393B (zh) * 2023-11-03 2024-03-19 杭州湃肽生化科技有限公司 一种植物组织中环肽的检测方法
CN117339580B (zh) * 2023-12-05 2024-04-02 凯莱英生命科学技术(天津)有限公司 螯合载体、其制备方法及应用
WO2025125266A1 (en) 2023-12-11 2025-06-19 Cube Biotech Gmbh Tablets containing fuctionalized agarose particles, method for producing them and use of the tablets
CN118888738B (zh) * 2024-09-30 2024-11-29 溧阳中科海钠科技有限责任公司 一种锡碳复合负极材料及其制备方法与应用

Family Cites Families (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4824986A (en) * 1985-04-26 1989-04-25 The United States Of America As Represented By The Secretary Of The Department Of Health And Human Services Metal chelate protein conjugate
CA1304886C (en) 1986-07-10 1992-07-07 Heinz Dobeli Metal chelate resins
GB8923843D0 (en) 1989-10-23 1989-12-13 Salutar Inc Compounds
GB9404208D0 (en) * 1994-03-04 1994-04-20 Nycomed Salutar Inc Chelants
CA2277372C (en) 1998-07-13 2011-03-15 Tran Quang Minh Affinity immobilised metal resins
US6623655B1 (en) 2000-04-24 2003-09-23 Sigma-Aldrich Co. Metal chelating compositions
SE0303532D0 (sv) * 2003-12-23 2003-12-23 Amersham Biosciences Ab Purification of immunoglobulins
US20050208118A1 (en) * 2004-03-18 2005-09-22 Takemoto Arnold C Preparations of encapsulated bioavailable chelating agents for detoxifying humans and animals
DE102004038134B4 (de) * 2004-08-05 2013-07-25 Johann Wolfgang Goethe-Universität Frankfurt am Main Multivalente Chelatoren zum Modifizieren und Organisieren von Zielmolekülen, Verfahren zu ihrer Herstellung sowie ihre Verwendung
JP2008508333A (ja) 2004-08-05 2008-03-21 ヨハン ウォルフガング ゲーテ−ウニベルジテート フランクフルト アム マイン 標的分子の改変および組織化のための多価キレーター
WO2009008802A1 (en) 2007-07-09 2009-01-15 Ge Healthcare Bio-Sciences Ab Method for preparation of a biomolecule adsorbent
EP2022561A1 (en) 2007-08-06 2009-02-11 Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V. Immobilisation of chelating groups for immobilised metal ion chromatography (IMAC)
ES2524786T5 (es) 2007-08-06 2019-06-19 Max Planck Gesellschaft Uso de quelantes inmovilizados para purificación de proteínas recombinantes por cromatografía de iones metálicos inmovilizados y método de purificación de proteínas recombinantes
GB0901207D0 (en) * 2009-01-26 2009-03-11 Innospec Ltd Chelating agents and methods relating thereto
EP2576502B1 (en) 2010-06-01 2020-08-05 Cytiva BioProcess R&D AB Novel chelator and use thereof
EP2995323B1 (de) * 2014-09-15 2019-02-27 Evonik Degussa GmbH Aminopolycarboxylsäuren als Prozesshilfsmittel bei der Superabsorberherstellung
WO2017046625A1 (en) 2015-06-25 2017-03-23 Cube Biotech Gmbh New chelators for affinity purification of recombinant proteins

Also Published As

Publication number Publication date
US12403461B2 (en) 2025-09-02
EP3887033B1 (en) 2024-07-24
JP2022509239A (ja) 2022-01-20
JP7544704B2 (ja) 2024-09-03
WO2020109162A1 (en) 2020-06-04
CA3121103A1 (en) 2020-06-04
EP3887033A1 (en) 2021-10-06
CN113164915A (zh) 2021-07-23
US20220023851A1 (en) 2022-01-27
DK3887033T3 (da) 2024-08-12

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