CN113156135B - 用于杆状病毒表达的亚单位抗原的标记系统及其用途 - Google Patents

用于杆状病毒表达的亚单位抗原的标记系统及其用途 Download PDF

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CN113156135B
CN113156135B CN202110085632.2A CN202110085632A CN113156135B CN 113156135 B CN113156135 B CN 113156135B CN 202110085632 A CN202110085632 A CN 202110085632A CN 113156135 B CN113156135 B CN 113156135B
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sequence
seq
virus
protein
insect cells
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CN113156135A (zh
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A·V·耶尔
J·R·赫尔曼
M·B·鲁夫
E·M·沃恩
M·L·舍费尔
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Boehringer Ingelheim Animal Health USA Inc
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CN202110085632.2A 2015-03-05 2016-03-04 用于杆状病毒表达的亚单位抗原的标记系统及其用途 Active CN113156135B (zh)

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US201562128744P 2015-03-05 2015-03-05
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PCT/US2016/021003 WO2016141338A2 (en) 2015-03-05 2016-03-04 Marker system, in particular for baculovirus-expressed subunit antigens
CN201680013955.8A CN107533066B (zh) 2015-03-05 2016-03-04 标记系统，特别是用于杆状病毒表达的亚单位抗原的标记系统

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CN113156135B (zh) * 2015-03-05 2025-01-07 勃林格殷格翰动物保健美国有限公司 用于杆状病毒表达的亚单位抗原的标记系统及其用途
CN108048476B (zh) 2017-11-21 2020-09-08 浙江迪福润丝生物科技有限公司 一种制备区分免疫和感染动物h9亚型禽流感疫苗株的方法及应用
US10718771B2 (en) 2018-02-09 2020-07-21 Chung Yuan Christian University Recombinant baculoviruses and their uses in detecting arthropod-borne virus
CN109536456B (zh) * 2018-10-26 2021-07-30 中国农业科学院哈尔滨兽医研究所（中国动物卫生与流行病学中心哈尔滨分中心） 识别pcv2病毒样颗粒的单克隆抗体及其在定性及定量检测pcv2病毒样颗粒中的应用
WO2021122896A1 (en) 2019-12-18 2021-06-24 Intervet International B.V. Cryptosporidiosis vaccine
JP2023513135A (ja) * 2020-02-06 2023-03-30 ベーリンガー インゲルハイム フェトメディカ ゲーエムベーハー 抗ラブドウイルス抗体を検出するのに有用なポリペプチド
CN115491377B (zh) * 2021-06-18 2025-09-16 北京三诺佳邑生物技术有限责任公司 一种诱导rna干扰和降低并清除细胞中病毒污染的核苷酸序列及应用
CN116064649B (zh) * 2022-11-24 2023-11-21 南京诺唯赞生物科技股份有限公司 一种在本氏烟草中表达猪瘟病毒e2蛋白的方法

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107533066A (zh) * 2015-03-05 2018-01-02 勃林格殷格翰动物保健公司 标记系统，特别是用于杆状病毒表达的亚单位抗原的标记系统

Family Cites Families (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5283173A (en) 1990-01-24 1994-02-01 The Research Foundation Of State University Of New York System to detect protein-protein interactions
JP2004506022A (ja) * 2000-08-11 2004-02-26 ファブリル　インコーポレイテッド Ｂ細胞媒介性病態を改変するための方法および組成物
US7833707B2 (en) 2004-12-30 2010-11-16 Boehringer Ingelheim Vetmedica, Inc. Methods of overexpression and recovery of porcine circovirus type 2 ORF2
US7700285B1 (en) * 2005-12-29 2010-04-20 Boehringer Ingelheim Vetmedica, Inc. PCV2 immunogenic compositions and methods of producing such compositions
CN101495139A (zh) 2005-11-10 2009-07-29 迪娃解决方案股份有限公司 鉴别治疗组合物
CN105769931B (zh) * 2006-09-15 2021-04-27 渥太华医院研究机构 溶瘤弹状病毒
EP2073818A4 (en) * 2006-10-18 2010-09-15 Us Gov Health & Human Serv IMPROVEMENT OF ILLNESS RESISTANCE TO RNA VIRUS INFECTIONS WITH INTRA-CYCLOPIC ENSURE SENSORS
US20090110698A1 (en) 2007-10-31 2009-04-30 Newport Laboratories, Inc. Method of determining vaccine compliance
WO2009079564A2 (en) * 2007-12-17 2009-06-25 Emory University Immunogenic compositions and methods of use thereof
AU2009256397B2 (en) * 2008-05-29 2014-06-05 The Government Of The United States Of America, As Represented By The Secretary, Department Of Health And Human Services Expression and assembly of human group C rotavirus-like particles and uses thereof
CN101884787A (zh) * 2010-07-22 2010-11-17 洛阳普莱柯生物工程有限公司 猪圆环病毒2型亚单位疫苗及其制备方法
NZ631110A (en) * 2012-08-29 2015-11-27 Intervet Int Bv Marker vaccine
KR102474663B1 (ko) 2013-10-03 2022-12-07 다케다 백신즈 인코포레이티드 세포주로부터 랍도바이러스를 탐지 및 제거하는 방법

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107533066A (zh) * 2015-03-05 2018-01-02 勃林格殷格翰动物保健公司 标记系统，特别是用于杆状病毒表达的亚单位抗原的标记系统

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
Immunological characeterization of the VAS nucleocapsid protein expressed by recombinant baculovirus in spodoptera exigua larva:Use in differential diagnosis between vaccinated and infected Animals;Shabbir A, et al;《Virology》;19930131;第192卷(第1期);第207-216页 *
水疱性口炎病毒两血清型G蛋白的短截表达和间接ELISA方法的建立;臧京帅，等;《中国兽医杂质》;20141231;第50卷(第6期);第69-71页 *

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