CN113151228A - Complex enzyme preparation for killing viruses and preparation method and application thereof - Google Patents
Complex enzyme preparation for killing viruses and preparation method and application thereof Download PDFInfo
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- CN113151228A CN113151228A CN202110282700.4A CN202110282700A CN113151228A CN 113151228 A CN113151228 A CN 113151228A CN 202110282700 A CN202110282700 A CN 202110282700A CN 113151228 A CN113151228 A CN 113151228A
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- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/58—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from fungi
- C12N9/62—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from fungi from Aspergillus
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/50—Isolated enzymes; Isolated proteins
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- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F11/00—Treatment of sludge; Devices therefor
- C02F11/004—Sludge detoxification
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- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
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- C02F11/00—Treatment of sludge; Devices therefor
- C02F11/02—Biological treatment
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- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
- C02F3/342—Biological treatment of water, waste water, or sewage characterised by the microorganisms used characterised by the enzymes used
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- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
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- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
- C12N9/0065—Oxidoreductases (1.) acting on hydrogen peroxide as acceptor (1.11)
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- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
- C12N9/22—Ribonucleases RNAses, DNAses
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- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
- C12N9/2462—Lysozyme (3.2.1.17)
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- C12Y111/00—Oxidoreductases acting on a peroxide as acceptor (1.11)
- C12Y111/01—Peroxidases (1.11.1)
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- C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
- C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
- C12Y302/01017—Lysozyme (3.2.1.17)
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- C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
- C12Y304/11—Aminopeptidases (3.4.11)
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- C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
- C12Y304/21—Serine endopeptidases (3.4.21)
- C12Y304/21004—Trypsin (3.4.21.4)
Abstract
The invention belongs to the technical field of sanitary protection and discloses a complex enzyme preparation for killing viruses and a preparation method and application thereof, wherein the complex enzyme preparation for killing the viruses comprises, by mass, 30-50 parts of an aspergillus oryzae culture, 40-60 parts of a complex enzyme, 10-15 parts of an enzyme stabilizer, 6-8 parts of a preservative and 3-5 parts of an auxiliary agent. The lysozyme is added into the complex enzyme preparation for killing the viruses, the cell structure of the mixed bacteria is destroyed by the lysozyme, the low-heat short-time thorough sterilization can be realized, the use amount of the killing medicines can be reduced, even the use of the killing medicines is completely removed, the complex enzyme preparation can be directly used for killing the viruses of various articles and environments, the use is safe and convenient, and the killing effect is obvious. The preparation method of the complex enzyme preparation for killing the viruses is simple, only culture and fermentation of strains are carried out, and the preparation cost of the complex enzyme preparation for killing the viruses can be reduced.
Description
Technical Field
The invention belongs to the technical field of sanitary protection, and particularly relates to a complex enzyme preparation for killing viruses and a preparation method and application thereof.
Background
At present, there are many ways of transmitting viruses, and the methods of transmitting easily caused infection include contact transmission, spray transmission, aerosol transmission, fecal oral transmission, and the like. In order to reduce various transmission risks, virus disinfection treatment is generally required for air environment, medical waste, domestic sewage of patients, excrement and urine and the like. However, the existing disinfectant has poor stability and is easy to decompose, or has strong irritation and corrosivity, or is not suitable for virus killing treatment of complex component background, or is easy to cause new environmental pollution, and the application range and the effect are both limited. Therefore, a new complex enzyme preparation for killing viruses and a preparation method thereof are needed.
Through the above analysis, the problems and defects of the prior art are as follows: the existing disinfectant has poor stability, is easy to decompose, or has strong irritation and corrosivity, or is not suitable for virus killing treatment of complex component background, or is easy to cause new environmental pollution, and the application range and the effect are both limited.
Disclosure of Invention
Aiming at the problems in the prior art, the invention provides a complex enzyme preparation for killing viruses and a preparation method and application thereof.
The invention is realized in such a way that the preparation method of the complex enzyme preparation for killing the viruses comprises the following steps:
step one, preparing a strain culture medium: weighing 40-50 parts of white sugar, 30-40 parts of agar, 20-25 parts of carrot powder, 15-20 parts of dipotassium hydrogen phosphate, 8-12 parts of magnesium sulfate and 4-8 parts of defoaming agent according to parts by mass; taking 1000ml of water, sequentially adding white sugar, agar and carrot powder into the water, uniformly stirring and heating to obtain a dissolving solution; cooling the dissolved solution to room temperature, adding dipotassium hydrogen phosphate, magnesium sulfate and an antifoaming agent, and uniformly stirring again to obtain a culture solution; sterilizing the culture solution at 110-120 ℃ for 20-30 min, and cooling to obtain a strain culture medium;
step two, culturing pure strains of aspergillus oryzae: placing the prepared strain culture medium in a slant test tube and placing the slant test tube on a slant test tube rack, wherein the specification of the slant test tube is 15cm multiplied by 15 mm; selecting pure strains of aspergillus oryzae to be inoculated into a strain culture medium in an aseptic environment, culturing the strains under the constant temperature condition of 20-25 ℃, wherein the culture time is 10-15 days, and obtaining the strains of the slant test tube after the culture is finished;
step three, culturing the seed culture by using the slant test tube strain obtained by culturing: dividing strains on a strain culture medium in a slant test tube obtained by culture into small blocks under an aseptic environment to obtain strain blocks, wherein the length of each strain block is 1.8-2.2 mm, and the width of each strain block is 1.8-2.2 mm; inoculating the divided strain blocks into a wood box culture vessel added with culture substances to prepare a seed starter; the culture substance consists of agar, peptone and malt extract;
step four, performing solid fermentation culture by using the prepared seed starter: inoculating the prepared seed starter to a strain culture medium in a fermentation tank for culture in an aseptic environment; performing aeration fermentation during the culturing process, wherein the aeration fermentation comprises: let in the air of purification in toward the fermentation cylinder to set up the air volume and be 1: 0.6; placing the fermentation tank on a shaking table, and setting the rotation speed of the shaking table to be 145-160 r/min; the pressure after purified air is introduced is 0.2-0.3 MPa, and the fermentation is carried out for 80-120 h under the condition of the temperature of 20-24 ℃ to complete ventilation fermentation; carrying out assay detection on the fermentation product, wherein the assay detection structure shows that the fermentation product reaching the preset standard is the Aspergillus oryzae culture;
step five, drying and crushing the prepared aspergillus oryzae culture, sequentially adding a complex enzyme, an enzyme stabilizer, a preservative and an auxiliary agent, uniformly stirring, sterilizing and cooling, wherein the sterilizing and cooling method comprises the following steps: steaming the mixture obtained by stirring under normal pressure; wherein the normal-pressure steaming is to sterilize the mixture for 3-5 h at 120-125 ℃ and 0.1-0.15 MPa; after steaming at normal pressure, steaming the mixture at high pressure; wherein the high-pressure steaming is to sterilize the mixture for 2-3 h at 110 ℃ and 80-120 MPa; after the material steaming and sterilization, cooling to room temperature to finish sterilization and cooling; obtaining the finished product of the complex enzyme preparation for killing the viruses.
The invention also aims to provide the complex enzyme preparation for killing the viruses, which is prepared by the preparation method of the complex enzyme preparation for killing the viruses, and the complex enzyme preparation for killing the viruses comprises 30-50 parts by mass of an aspergillus oryzae culture, 40-60 parts by mass of the complex enzyme, 10-15 parts by mass of an enzyme stabilizer, 6-8 parts by mass of a preservative and 3-5 parts by mass of an auxiliary agent.
Further, the compound enzyme is calculated by the unit of the activity of enzyme, and is prepared from protease K, keratinase, aminopeptidase, trypsin, broad-spectrum nuclease, lysozyme, proteolytic enzyme, staphylococcal enzyme and peroxidase according to the ratio of (40-1000): (40-1000): (30-100): (30-100): (10-100): (10-100): (5-100): (0.1-100): (0.1-50).
Further, the nuclease and the lysozyme are coated with an encapsulation material; the encapsulating material comprises maltodextrin and chitosan, and the dosage ratio of the maltodextrin to the chitosan in the encapsulating material is (2-5): 1.
further, the enzyme stabilizer comprises the following components in percentage by mass: glycerol: propylene glycol: boric acid 800: 300: 1.
further, the preservative is calcium propionate, and the auxiliary agent comprises ethanol, essence and pigment.
The invention also aims to provide application of the complex enzyme preparation for killing viruses in article or environment virus killing, wherein the article comprises textiles, and the environment comprises indoor environment and outdoor environment.
Further, the application of the complex enzyme preparation for killing the viruses in killing the environmental viruses comprises the following steps: garbage treatment, manure treatment, air treatment or sewage treatment.
Further, the virus includes: foamy virus, influenza virus, parainfluenza virus, respiratory syncytial virus, herpes simplex virus, cytomegalovirus, murine stem cell virus, human immunodeficiency virus, SARS virus, members of the coronavirus family, human post-pneumonia virus, carapau arthritis encephalitis virus, and Epstein-Bar virus.
Further, the application method of the complex enzyme preparation for killing the viruses comprises the following steps:
adding sterilized distilled water into the complex enzyme preparation, and dissolving into enzyme solution for later use;
using equipment to make air pass through enzyme solution to kill virus; wherein the equipment is a spray tower.
By combining all the technical schemes, the invention has the advantages and positive effects that: the lysozyme is added into the complex enzyme preparation for killing the viruses, the cell structure of the mixed bacteria is destroyed by the lysozyme, the low-heat short-time thorough sterilization can be realized, the use amount of the killing medicines can be reduced, even the use of the killing medicines is completely removed, the complex enzyme preparation can be directly used for killing the viruses of various articles and environments, the use is safe and convenient, and the killing effect is obvious. The preparation method of the complex enzyme preparation for killing the viruses is simple, only culture and fermentation of strains are carried out, and the preparation cost of the complex enzyme preparation for killing the viruses can be reduced.
Drawings
In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings needed to be used in the embodiments of the present invention will be briefly described below, and it is obvious that the drawings described below are only some embodiments of the present invention, and it is obvious for those skilled in the art that other drawings can be obtained according to the drawings without creative efforts.
FIG. 1 is a flow chart of a method for preparing a complex enzyme preparation for killing viruses according to an embodiment of the present invention.
FIG. 2 is a flow chart of the preparation of a seed culture medium according to an embodiment of the present invention.
FIG. 3 is a flow chart of the process of performing aeration fermentation according to the embodiment of the present invention.
Fig. 4 is a flow chart of sterilization and cooling provided by an embodiment of the present invention.
FIG. 5 is a flow chart of an application method of the complex enzyme preparation for virus killing provided by the embodiment of the invention.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention is further described in detail with reference to the following embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention.
Aiming at the problems in the prior art, the invention provides a complex enzyme preparation for killing viruses and a preparation method and application thereof, and the invention is described in detail below by combining the attached drawings.
As shown in fig. 1, the preparation method of the complex enzyme preparation for killing viruses provided by the embodiment of the invention comprises the following steps:
s101, culturing pure strains of aspergillus oryzae: preparing a strain culture medium, namely placing the prepared strain culture medium in a slant test tube and placing the slant test tube on a slant test tube rack, wherein the specification of the slant test tube is 15cm multiplied by 15 mm; in an aseptic environment, selecting pure strains of aspergillus oryzae, inoculating the pure strains into a strain culture medium, culturing the strains at a constant temperature of 20-25 ℃ for 10-15 days, and obtaining the strains in the test tube on the inclined plane after the culture is finished.
S102, culturing the seed culture by using the slant test tube strain obtained by culturing: dividing strains on a strain culture medium in a slant test tube obtained by culture into small blocks under an aseptic environment to obtain strain blocks, wherein the length of each strain block is 1.8-2.2 mm, and the width of each strain block is 1.8-2.2 mm; inoculating the divided strain blocks into a wood box culture vessel added with culture substances to prepare a seed starter; the culture material is composed of agar, peptone and malt extract.
S103, performing solid fermentation culture by using the prepared seed starter: inoculating the prepared seed starter to a strain culture medium in a fermentation tank for culture in an aseptic environment; ventilating and fermenting in the culture process; and (4) carrying out assay detection on the fermentation product, wherein the assay detection structure shows that the fermentation product reaching the preset standard is the Aspergillus oryzae culture.
S104, drying and crushing the prepared Aspergillus oryzae culture, sequentially adding a complex enzyme, an enzyme stabilizer, a preservative and an auxiliary agent, uniformly stirring, sterilizing and cooling to obtain a finished product of the complex enzyme preparation for killing the viruses.
As shown in fig. 2, the preparation of the strain medium provided by the embodiment of the present invention includes:
s201, weighing 40-50 parts of white sugar, 30-40 parts of agar, 20-25 parts of carrot powder, 15-20 parts of dipotassium hydrogen phosphate, 8-12 parts of magnesium sulfate and 4-8 parts of defoaming agent according to parts by weight.
S202, taking 1000ml of water, sequentially adding white sugar, agar and carrot powder into the water, uniformly stirring and heating to obtain a dissolving solution.
And S203, cooling the dissolved solution to room temperature, adding dipotassium hydrogen phosphate, magnesium sulfate and an antifoaming agent, and uniformly stirring again to obtain a culture solution.
S204, sterilizing the culture solution at 110-120 ℃ for 20-30 min, and cooling to obtain the strain culture medium.
As shown in fig. 3, the fermentation with aeration according to the embodiment of the present invention includes:
s301, let in the air of purification in toward the fermentation cylinder to set up the air volume and be 1: 0.6.
s302, placing the fermentation tank on a shaking table, and setting the rotation speed of the shaking table to 145-160 r/min.
S303, introducing purified air, culturing for 80-120 h at the temperature of 20-24 ℃ under the pressure of 0.2-0.3 MPa, and completing ventilation fermentation.
As shown in fig. 4, the sterilization and cooling provided by the embodiment of the present invention includes:
s401, steaming the mixture obtained by stirring at normal pressure; wherein the normal-pressure steaming is to sterilize the mixture for 3-5 hours at 120-125 ℃ and 0.1-0.15 MPa.
S402, steaming the mixture at normal pressure, and then steaming the mixture at high pressure; wherein the high-pressure steaming is to sterilize the mixture for 2-3 hours at 110 ℃ and under the pressure of 80-120 MPa.
And S403, after the material is steamed and sterilized, cooling to room temperature, and completing sterilization and cooling.
The compound enzyme preparation for killing the viruses provided by the embodiment of the invention comprises, by mass, 30-50 parts of an Aspergillus oryzae culture, 40-60 parts of a compound enzyme, 10-15 parts of an enzyme stabilizer, 6-8 parts of a preservative and 3-5 parts of an auxiliary agent.
The complex enzyme provided by the embodiment of the invention is prepared from protease K, keratinase, aminopeptidase, trypsin, broad-spectrum nuclease, lysozyme, proteolytic enzyme, staphylococcal enzyme and peroxidase according to the following ratio (40-1000): (40-1000): (30-100): (30-100): (10-100): (10-100): (5-100): (0.1-100): (0.1-50).
The nuclease and the lysozyme provided by the embodiment of the invention are coated by an encapsulating material; the encapsulating material comprises maltodextrin and chitosan, and the dosage ratio of the maltodextrin to the chitosan in the encapsulating material is (2-5): 1.
the enzyme stabilizer provided by the embodiment of the invention comprises the following components in percentage by mass: glycerol: propylene glycol: boric acid 800: 300: 1.
the preservative provided by the embodiment of the invention is calcium propionate, and the auxiliary agent comprises ethanol, essence and pigment.
The application of the complex enzyme preparation for killing the viruses provided by the embodiment of the invention in killing the environmental viruses comprises the following steps: garbage treatment, manure treatment, air treatment or sewage treatment.
The virus provided by the embodiment of the invention comprises: foamy virus, influenza virus, parainfluenza virus, respiratory syncytial virus, herpes simplex virus, cytomegalovirus, murine stem cell virus, human immunodeficiency virus, SARS virus, members of the coronavirus family, human post-pneumonia virus, carapau arthritis encephalitis virus, and Epstein-Bar virus.
As shown in fig. 5, the application method of the complex enzyme preparation for virus killing provided by the embodiment of the invention comprises the following steps:
s501, adding sterilized distilled water into the complex enzyme preparation, and dissolving into enzyme solution for later use;
s502, using equipment to enable air to pass through enzyme liquid for virus killing; wherein the equipment is a spray tower.
The above description is only for the purpose of illustrating the present invention and the appended claims are not to be construed as limiting the scope of the invention, which is intended to cover all modifications, equivalents and improvements that are within the spirit and scope of the invention as defined by the appended claims.
Claims (10)
1. A preparation method of a complex enzyme preparation for killing viruses is characterized by comprising the following steps:
step one, preparing a strain culture medium: weighing 40-50 parts of white sugar, 30-40 parts of agar, 20-25 parts of carrot powder, 15-20 parts of dipotassium hydrogen phosphate, 8-12 parts of magnesium sulfate and 4-8 parts of defoaming agent according to parts by mass; taking 1000ml of water, sequentially adding white sugar, agar and carrot powder into the water, uniformly stirring and heating to obtain a dissolving solution; cooling the dissolved solution to room temperature, adding dipotassium hydrogen phosphate, magnesium sulfate and an antifoaming agent, and uniformly stirring again to obtain a culture solution; sterilizing the culture solution at 110-120 ℃ for 20-30 min, and cooling to obtain a strain culture medium;
step two, culturing pure strains of aspergillus oryzae: placing the prepared strain culture medium in a slant test tube and placing the slant test tube on a slant test tube rack, wherein the specification of the slant test tube is 15cm multiplied by 15 mm; selecting pure strains of aspergillus oryzae to be inoculated into a strain culture medium in an aseptic environment, culturing the strains under the constant temperature condition of 20-25 ℃, wherein the culture time is 10-15 days, and obtaining the strains of the slant test tube after the culture is finished;
step three, culturing the seed culture by using the slant test tube strain obtained by culturing: dividing strains on a strain culture medium in a slant test tube obtained by culture into small blocks under an aseptic environment to obtain strain blocks, wherein the length of each strain block is 1.8-2.2 mm, and the width of each strain block is 1.8-2.2 mm; inoculating the divided strain blocks into a wood box culture vessel added with culture substances to prepare a seed starter; the culture substance consists of agar, peptone and malt extract;
step four, performing solid fermentation culture by using the prepared seed starter: inoculating the prepared seed starter to a strain culture medium in a fermentation tank for culture in an aseptic environment; performing aeration fermentation during the culturing process, wherein the aeration fermentation comprises: let in the air of purification in toward the fermentation cylinder to set up the air volume and be 1: 0.6; placing the fermentation tank on a shaking table, and setting the rotation speed of the shaking table to be 145-160 r/min; the pressure after purified air is introduced is 0.2-0.3 MPa, and the fermentation is carried out for 80-120 h under the condition of the temperature of 20-24 ℃ to complete ventilation fermentation; carrying out assay detection on the fermentation product, wherein the assay detection structure shows that the fermentation product reaching the preset standard is the Aspergillus oryzae culture;
step five, drying and crushing the prepared aspergillus oryzae culture, sequentially adding a complex enzyme, an enzyme stabilizer, a preservative and an auxiliary agent, uniformly stirring, sterilizing and cooling, wherein the sterilizing and cooling method comprises the following steps: steaming the mixture obtained by stirring under normal pressure; wherein the normal-pressure steaming is to sterilize the mixture for 3-5 h at 120-125 ℃ and 0.1-0.15 MPa; after steaming at normal pressure, steaming the mixture at high pressure; wherein the high-pressure steaming is to sterilize the mixture for 2-3 h at 110 ℃ and 80-120 MPa; after the material steaming and sterilization, cooling to room temperature to finish sterilization and cooling; obtaining the finished product of the complex enzyme preparation for killing the viruses.
2. The complex enzyme preparation for killing the viruses, which is prepared by the preparation method of the complex enzyme preparation for killing the viruses according to claim 1, is characterized by comprising 30-50 parts by mass of an aspergillus oryzae culture, 40-60 parts by mass of the complex enzyme, 10-15 parts by mass of an enzyme stabilizer, 6-8 parts by mass of a preservative and 3-5 parts by mass of an auxiliary agent.
3. A complex enzyme preparation for viral eradication according to claim 2, wherein the complex enzyme is composed of proteinase K, keratinase, aminopeptidase, trypsin, broad-spectrum nuclease, lysozyme, proteolytic enzyme, staphylococcal enzyme and peroxidase in terms of units of enzyme activity in a ratio of (40-1000): (40-1000): (30-100): (30-100): (10-100): (10-100): (5-100): (0.1-100): (0.1-50).
4. A complex enzyme preparation for viral eradication according to claim 3, wherein the nuclease and the lysozyme are coated with an encapsulation material; the encapsulating material comprises maltodextrin and chitosan, and the dosage ratio of the maltodextrin to the chitosan in the encapsulating material is (2-5): 1.
5. the complex enzyme preparation for killing viruses as claimed in claim 2, wherein the mass ratio of the enzyme stabilizer is as follows: glycerol: propylene glycol: boric acid 800: 300: 1.
6. a complex enzyme preparation for killing viruses as claimed in claim 2, wherein the preservative is calcium propionate and the auxiliaries comprise ethanol, essence and pigment.
7. Use of a complex enzyme preparation for viral disinfection according to any of claims 2-6 in the disinfection of goods comprising textiles or of environmental viruses, said environment comprising an indoor environment and an outdoor environment.
8. The use of a complex enzyme preparation for viral disinfection as claimed in claim 7 for disinfection of goods or environmental viruses, wherein the use of the complex enzyme preparation for viral disinfection in environmental viral disinfection comprises: garbage treatment, manure treatment, air treatment or sewage treatment.
9. The use of a complex enzyme preparation for the disinfection of viruses according to claim 7 for the disinfection of goods or environmental viruses, wherein said viruses comprise: foamy virus, influenza virus, parainfluenza virus, respiratory syncytial virus, herpes simplex virus, cytomegalovirus, murine stem cell virus, human immunodeficiency virus, SARS virus, members of the coronavirus family, human post-pneumonia virus, carapau arthritis encephalitis virus, and Epstein-Bar virus.
10. The use of a complex enzyme preparation for viral eradication according to claim 7 in the eradication of an article or an environmental virus, wherein the method for using the complex enzyme preparation for viral eradication comprises:
adding sterilized distilled water into the complex enzyme preparation, and dissolving into enzyme solution for later use;
using equipment to make air pass through enzyme solution to kill virus; wherein the equipment is a spray tower.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110282700.4A CN113151228A (en) | 2021-03-16 | 2021-03-16 | Complex enzyme preparation for killing viruses and preparation method and application thereof |
Applications Claiming Priority (1)
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