CN113150983A - Biological cell culture ware - Google Patents
Biological cell culture ware Download PDFInfo
- Publication number
- CN113150983A CN113150983A CN202110254183.XA CN202110254183A CN113150983A CN 113150983 A CN113150983 A CN 113150983A CN 202110254183 A CN202110254183 A CN 202110254183A CN 113150983 A CN113150983 A CN 113150983A
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- Prior art keywords
- side wall
- fixedly connected
- block
- fixed
- cell culture
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
- C12M41/36—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of biomass, e.g. colony counters or by turbidity measurements
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
- C12M27/16—Vibrating; Shaking; Tilting
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/06—Nozzles; Sprayers; Spargers; Diffusers
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
- C12M33/04—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by injection or suction, e.g. using pipettes, syringes, needles
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Sustainable Development (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Molecular Biology (AREA)
- Analytical Chemistry (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention discloses a biological cell culture device, which comprises a protective shell, wherein a fixed base is fixedly connected on the side wall of the protective shell, first springs are symmetrically and fixedly connected on the side wall of the fixed base along the central axis of the fixed base, the free ends of the first springs are fixedly connected with a supporting plate together, the supporting plate is in sliding connection with the side wall of the fixed base, a carrier is arranged on the side wall of the supporting plate, a driving motor is fixedly connected on the side wall of the protective shell, a transmission shaft is fixedly connected at the tail end of an output shaft of the driving motor, in the process that a connecting pipe is extruded by an extrusion wheel ceaselessly, outside air is conveyed into the carrier, the air can float up to the surface of the carrier, the process realizes the even distribution of cells, simultaneously provides oxygen for the cells, is convenient for the growth of the cells, and does not need manual operation, a large amount of manpower labor is reduced, and the cell culture speed is increased.
Description
Technical Field
The invention relates to the technical field of cell culture, in particular to a biological cell culture device.
Background
Cell culture refers to a method of simulating in vivo environment (sterile, proper temperature, pH value, certain nutritional conditions, etc.) in vitro to enable the cells to survive, grow, reproduce and maintain the main structure and function. Cell culture is also called cell cloning technology, and the formal term in biology is cell culture technology. Cell culture is an essential process for both the whole bioengineering technique and one of the biological cloning techniques, and is itself a large-scale cloning of cells.
In general, a culture dish or a glass ware is used as a carrier for cell culture, and cells are cultured and grown by a nutrient medium.
Traditional cell culture needs the manual work to equally divide the inside cell of carrier, thereby make more even of its growth, but this process needs the manual work to carry out continuous shock to the carrier and rocks, and then extravagant plenty of time and manpower, and the in-process of cell culture needs to detect the cell quantity of the inside unit volume of carrier, traditional detection mode is to open the carrier and take a sample again and detect, but this process not only can make outside dust enter into the carrier inside and cause the damage to the cell, simultaneously because cell growth is not enough even, thereby can't carry out accurate estimation to the quantity of unit volume cell, consequently, a biological cell culture ware is needed.
Disclosure of Invention
The invention aims to solve the defects in the prior art and provides a biological cell culture device.
In order to achieve the purpose, the invention adopts the following technical scheme:
a biological cell culture device comprises a protective shell, wherein a fixed base is fixedly connected to the side wall of the protective shell, first springs are symmetrically and fixedly connected to the side wall of the fixed base along the central axis of the fixed base, a supporting plate is fixedly connected to the free ends of the first springs together, the supporting plate is in sliding connection with the side wall of the fixed base, a carrier is arranged on the side wall of the supporting plate, a driving motor is fixedly connected to the side wall of the protective shell, a transmission shaft is fixedly connected to the tail end of an output shaft of the driving motor, a first bevel gear is fixedly connected to the side wall of the transmission shaft, an extrusion mechanism is fixedly mounted on the side wall of the protective shell, the extrusion mechanism comprises an extrusion box fixedly connected to the side wall of the protective shell, a connecting rod is rotatably connected to the side wall of the extrusion box, and the connecting rod penetrates from the inside of the extrusion box to the outside of the extrusion box, the tail end of the connecting rod is fixedly connected with a second bevel gear, the side wall of the extrusion box is provided with a positioning groove, the side wall of the positioning groove is fixedly provided with a connecting pipe, one end of the connecting pipe extends into the inside of the carrier, the other end of the connecting pipe penetrates from the inside of the protective shell to the outside of the protective shell, the side wall of the connecting rod is fixedly connected with a connecting wheel, the side wall of the connecting wheel is symmetrically and fixedly connected with a plurality of locking rods along the center of the connecting wheel, the side wall of each locking rod is rotatably connected with the extrusion wheel, the extrusion wheel is tightly abutted against the side wall of the connecting pipe, the side wall of the transmission shaft is fixedly connected with a driving mechanism, the side wall of the supporting plate is fixedly connected with a locking block, the inside of the locking block is fixedly provided with a first magnetic block, the side wall of the protective shell is fixedly connected with a fixing, fixedly connected with actuating lever on the end of transmission shaft, fixedly connected with sleeve on the lateral wall of fixed case, fixedly connected with gasbag on the telescopic lateral wall, fixedly connected with drive block on the lateral wall of gasbag, drive block and telescopic lateral wall sliding connection, fixedly connected with rope on the lateral wall of drive block, the rope runs through to the outside of fixed case by the inside of fixed case, the spacing groove has been seted up on the lateral wall of connecting pipe, sliding connection has the limiting plate on the lateral wall of spacing groove, fixedly connected with second spring on the lateral wall of limiting plate, the end of second spring and the lateral wall fixed connection of connecting pipe, the end of rope and the lateral wall fixed connection of limiting plate.
Preferably, actuating mechanism includes the fixed block of fixed connection on the transmission shaft lateral wall, the inside fixed mounting of fixed block has the second magnetic block, the magnetism of second magnetic block and first magnetic block is opposite.
Preferably, the first bevel gear is meshed with the second bevel gear, and the first bevel gear and the second bevel gear are both made of austenitic stainless steel.
Preferably, the second spring is wound around the side wall of the limiting plate, and the limiting plate is tightly attached to the side wall of the connecting pipe.
Preferably, a supporting base is fixedly installed at the bottom of the protection casing.
Preferably, the cord is in a slack state.
Preferably, the first spring is made of spring steel, a clamping groove is formed in the side wall of the supporting plate, and the carrier is placed inside the clamping groove.
Compared with the prior art, the invention has the beneficial effects that:
1. the carrier is placed above the supporting plate, the driving motor is started at the moment, the output shaft of the driving motor drives the transmission shaft to start rotating, the transmission shaft rotates to enable the fixed block to start rotating, the second magnetic block inside the fixed block is opposite to the first magnetic block inside the locking block in magnetism, so that outward thrust can be exerted on the locking block in the rotating process of the fixed block, the supporting plate is enabled to move back and forth under the action of the first spring, the carrier is enabled to move back and forth to achieve uniform distribution of cells inside the carrier, the first bevel gear can start rotating through the transmission shaft, the first bevel gear is meshed with the second bevel gear, so that the connecting rod starts rotating, the connecting rod drives the connecting wheel to start rotating, the connecting wheel enables the extrusion wheel to start rotating through the locking rod, the extrusion wheel is enabled to abut against the side wall of the connecting pipe, and in the process that the extrusion wheel extrudes the connecting pipe ceaselessly, outside air is conveyed to the carrier, the air can float to the surface of the carrier, the process realizes the even distribution of the cells, simultaneously provides oxygen for the cells, is convenient for the growth of the cells, does not need manual operation, lightens a large amount of manual labor, and increases the speed of cell culture.
2. Need detect the cell of the inside unit volume of carrier at the in-process that carries out the cultivation to the cell, start driving motor, make driving motor antiport, thereby make transmission shaft antiport, the transmission shaft rotates and makes first bevel gear antiport, and then make connecting rod antiport, because the lateral wall of extrusion wheel and connecting pipe offsets tightly, can extract the cell of the inside of carrier through the connecting pipe at this in-process, whole process need not open the carrier, avoid the damage of dust impurity to the cell, the fixed block also can rotate along with the extrusion wheel simultaneously, thereby vibrate the equipartition to the cell of the inside of carrier, make the measurement to the cell quantity in the unit volume more accurate.
Drawings
FIG. 1 is a schematic structural view of a biological cell culture apparatus according to the present invention;
FIG. 2 is an enlarged view of part A of the present invention;
FIG. 3 is an enlarged view of portion B of the present invention;
FIG. 4 is an enlarged view of portion C of the present invention;
FIG. 5 is an enlarged view of portion D of the present invention;
FIG. 6 is a schematic structural diagram of a pressurizing structure according to the present invention.
In the figure: the device comprises a protective shell 1, a fixed base 2, a first spring 3, a supporting plate 4, a carrier 5, a connecting pipe 6, a driving motor 7, a transmission shaft 8, a first bevel gear 9, a second bevel gear 10, a connecting rod 11, a fixed box 12, a squeezing box 13, a positioning groove 14, a connecting wheel 15, a locking rod 16, a squeezing wheel 17, a first magnetic block 18, a fixed block 19, a second magnetic block 20, a sleeve 21, an air bag 22, a driving block 23, a driving rod 24, a rope 25, a limiting groove 26, a limiting plate 27, a second spring 28 and a locking block 29.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments.
Referring to fig. 1-6, a biological cell culture device comprises a protective housing 1, a fixed base 2 is fixedly connected to the side wall of the protective housing 1, first springs 3 are symmetrically and fixedly connected to the side wall of the fixed base 2 along the central axis of the fixed base 2, the first springs 3 are made of spring steel, a support plate 4 is fixedly connected to the free ends of the first springs 3, the support plate 4 is slidably connected to the side wall of the fixed base 2, a carrier 5 is arranged on the side wall of the support plate 4, a clamping groove is formed in the side wall of the support plate 4, the carrier 5 is placed in the clamping groove, a driving motor 7 is fixedly connected to the side wall of the protective housing 1, a transmission shaft 8 is fixedly connected to the tail end of an output shaft of the driving motor 7, and a first bevel gear 9 is fixedly connected to the side wall of the transmission shaft 8;
the side wall of the protective shell 1 is fixedly provided with an extrusion mechanism, the extrusion mechanism comprises an extrusion box 13 fixedly connected to the side wall of the protective shell 1, the side wall of the extrusion box 13 is rotatably connected with a connecting rod 11, the connecting rod 11 penetrates from the inside of the extrusion box 13 to the outside of the extrusion box 13, the tail end of the connecting rod 11 is fixedly connected with a second bevel gear 10, a first bevel gear 9 is meshed with the second bevel gear 10, the first bevel gear 9 and the second bevel gear 10 are both made of austenitic stainless steel, the austenitic stainless steel is stable in structure and is not easy to rust, the connecting rod can be used for a long time without replacement, the side wall of the extrusion box 13 is provided with a positioning groove 14, the side wall of the positioning groove 14 is fixedly provided with a connecting pipe 6, one end of the connecting pipe 6 penetrates into the carrier 5, and the other end of the connecting pipe 6 penetrates from the inside of the protective shell 1 to the outside of the protective shell 1, a connecting wheel 15 is fixedly connected to the side wall of the connecting rod 11, a plurality of locking rods 16 are symmetrically and fixedly connected to the side wall of the connecting wheel 15 along the center of the connecting wheel 15, an extrusion wheel 17 is rotatably connected to the side wall of each locking rod 16, and the extrusion wheel 17 is abutted against the side wall of the connecting pipe 6;
the side wall of the transmission shaft 8 is fixedly connected with a driving mechanism, the driving mechanism comprises a fixed block 19 fixedly connected to the side wall of the transmission shaft 8, a second magnetic block 20 is fixedly mounted inside the fixed block 19, the magnetism of the second magnetic block 20 is opposite to that of the first magnetic block 18, and the carrier 5 can shake back and forth through the driving mechanism, so that cells inside the carrier 5 are uniformly distributed, manual oscillation is not needed, the operation is faster, the sampling can be more uniform in the subsequent sampling process, and the obtained cell data of unit volume is more accurate;
a locking block 29 is fixedly connected to the side wall of the supporting plate 4, a first magnetic block 18 is fixedly installed inside the locking block 29, a fixing box 12 is fixedly connected to the side wall of the protection casing 1, the end of the transmission shaft 8 penetrates into the fixing box 12 from the outside of the fixing box 12, a driving rod 24 is fixedly connected to the end of the transmission shaft 8, a sleeve 21 is fixedly connected to the side wall of the fixing box 12, an air bag 22 is fixedly connected to the side wall of the sleeve 21, a driving block 23 is fixedly connected to the side wall of the air bag 22, the driving block 23 is slidably connected to the side wall of the sleeve 21, a rope 25 is fixedly connected to the side wall of the driving block 23, the rope 25 is in a loose state, the rope 25 penetrates into the outside of the fixing box 12 from the inside of the fixing box 12, a limiting groove 26 is formed in the side wall of the connecting pipe 6, a limiting plate 27 is slidably connected to the side wall of the limiting groove 26, and a second spring 28 is fixedly connected to the side wall of the limiting plate 27, the terminal lateral wall fixed connection with connecting pipe 6 of second spring 28, the terminal lateral wall fixed connection with limiting plate 27 of rope 25, second spring 28 twines mutually with limiting plate 27's lateral wall, limiting plate 27 closely laminates with connecting pipe 6's lateral wall, can avoid external bacterium to enter into 5 inside at the cultivation in-process, the bottom fixed mounting of protection casing 1 has the support base, and at cell culture's in-process actuating lever 24 not contact with the drive block, make connecting pipe 6 be in encapsulated situation.
In the invention, when the carrier 5 is placed above the supporting plate 4, the driving motor 7 is started, the output shaft of the driving motor 7 drives the transmission shaft 8 to rotate, the transmission shaft 8 rotates to enable the fixing block 19 to rotate, since the second magnetic block 20 inside the fixed block 19 is opposite to the first magnetic block 18 inside the locking block 29, there will be an outward pushing force on the locking block 29 during the rotation of the fixed block 19, the support plate 4 is moved back and forth under the action of the first spring 3, the support plate 4 makes the carrier 5 move back and forth to realize the uniform distribution of cells in the carrier 5, the drive shaft 8 can make the drive rod 24 start to rotate, when the drive rod 24 is contacted with the drive block 23, the driving block 23 retracts towards the interior of the sleeve 21 to extrude the air bag 22, the rope 25 is pulled by the driving block 23 in the retracting process, and the limiting plate 27 is pulled by the rope 25 to move outwards to open the connecting pipe 6;
the transmission shaft 8 enables the first bevel gear 9 to start to rotate, the first bevel gear 9 is meshed with the second bevel gear 10, the connecting rod 11 starts to rotate, the connecting rod 11 drives the connecting wheel 15 to start to rotate, the connecting wheel 15 enables the extrusion wheel 17 to start to rotate through the locking rod 16, the extrusion wheel 17 abuts against the side wall of the connecting pipe 6 tightly, external air is conveyed to the carrier 5 in the process that the extrusion wheel 17 continuously extrudes the connecting pipe 6, the air can float to the surface of the carrier 5, cell equalization is achieved in the process, meanwhile, oxygen is provided for cells, cell growth is facilitated, cells need to be oscillated and equalized manually, a large amount of manpower and time are reduced, and the cell culture speed is increased;
in the process of culturing, the number of cells in the unit volume inside the carrier 5 needs to be detected, the driving motor 7 is started, the output shaft of the driving motor 7 reversely rotates, so that the transmission shaft 8 reversely rotates, the transmission shaft 8 rotates to reversely rotate the first bevel gear 9, further the connecting rod 11 reversely rotates, the transmission shaft 8 can also reversely rotate the driving rod 24, when the driving rod 24 contacts with the driving block 23, the driving block 23 retracts into the sleeve 21, the driving block 23 can pull the rope 25 in the retracting process, the rope 25 pulls the limiting plate 27 to move outwards to open the connecting pipe 6 again, because the extrusion wheel 17 is tightly abutted against the side wall of the connecting pipe 6, in the process of reversely extruding the connecting pipe 6, the cells inside the carrier 5 can be absorbed through the connecting pipe 6, the carrier 5 does not need to be opened in the whole process, avoid the damage of dust impurity to the cell, fixed block 19 also can be to rotating along with extrusion wheel 17 simultaneously to vibrate equally divide to the cell of carrier 5 inside, make the measurement to the cell quantity in the unit volume more accurate.
The above description is only for the preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art should be considered to be within the technical scope of the present invention, and the technical solutions and the inventive concepts thereof according to the present invention should be equivalent or changed within the scope of the present invention.
Claims (7)
1. A biological cell culture device comprises a protective shell (1) and is characterized in that a fixed base (2) is fixedly connected onto the side wall of the protective shell (1), first springs (3) are fixedly connected onto the side wall of the fixed base (2) symmetrically along the central axis of the fixed base (2), a support plate (4) is fixedly connected onto the free end of each first spring (3) together, the support plate (4) is in sliding connection with the side wall of the fixed base (2), a carrier (5) is arranged on the side wall of the support plate (4), a driving motor (7) is fixedly connected onto the side wall of the protective shell (1), a transmission shaft (8) is fixedly connected onto the tail end of an output shaft of the driving motor (7), a first bevel gear (9) is fixedly connected onto the side wall of the transmission shaft (8), and an extrusion mechanism is fixedly mounted onto the side wall of the protective shell (1), the extrusion mechanism comprises an extrusion box (13) fixedly connected to the side wall of the protective shell (1), a connecting rod (11) is rotatably connected to the side wall of the extrusion box (13), the connecting rod (11) penetrates through the extrusion box (13) from the inside to the outside of the extrusion box (13), a second bevel gear (10) is fixedly connected to the tail end of the connecting rod (11), a positioning groove (14) is formed in the side wall of the extrusion box (13), a connecting pipe (6) is fixedly mounted on the side wall of the positioning groove (14), one end of the connecting pipe (6) penetrates into the carrier (5), the other end of the connecting pipe (6) penetrates through the protection shell (1) from the inside to the outside of the protective shell (1), a connecting wheel (15) is fixedly connected to the side wall of the connecting rod (11), and a plurality of locking rods (16) are symmetrically and fixedly connected to the side wall of the connecting wheel (15) along the center of the connecting wheel (15), the side wall of the locking rod (16) is rotatably connected with an extrusion wheel (17), the extrusion wheel (17) is abutted against the side wall of the connecting pipe (6), the side wall of the transmission shaft (8) is fixedly connected with a driving mechanism, the side wall of the supporting plate (4) is fixedly connected with a locking block (29), the inside of the locking block (29) is fixedly provided with a first magnetic block (18), the side wall of the protection shell (1) is fixedly connected with a fixed box (12), the tail end of the transmission shaft (8) penetrates through the inside of the fixed box (12) from the outside of the fixed box (12), the tail end of the transmission shaft (8) is fixedly connected with a driving rod (24), the side wall of the fixed box (12) is fixedly connected with a sleeve (21), the side wall of the sleeve (21) is fixedly connected with an air bag (22), and the side wall of the air bag (22) is fixedly connected with a driving block (23), the lateral wall sliding connection of drive block (23) and sleeve (21), fixedly connected with rope (25) on the lateral wall of drive block (23), rope (25) run through to the outside of fixed case (12) by the inside of fixed case (12), spacing groove (26) have been seted up on the lateral wall of connecting pipe (6), sliding connection has limiting plate (27) on the lateral wall of spacing groove (26), fixedly connected with second spring (28) on the lateral wall of limiting plate (27), the terminal of second spring (28) and the lateral wall fixed connection of connecting pipe (6), the terminal of rope (25) and the lateral wall fixed connection of limiting plate (27).
2. A biological cell culture device according to claim 1, wherein the driving mechanism comprises a fixed block (19) fixedly connected to the side wall of the transmission shaft (8), a second magnetic block (20) is fixedly arranged inside the fixed block (19), and the second magnetic block (20) has a magnetic property opposite to that of the first magnetic block (18).
3. The biological cell culture device according to claim 2, wherein the first bevel gear (9) is engaged with the second bevel gear (10), and the first bevel gear (9) and the second bevel gear (10) are both made of austenitic stainless steel.
4. A biological cell incubator as claimed in claim 3, characterised in that said second spring (28) is wound around the side wall of the plate (27) of restriction, said plate (27) of restriction being in close abutment with the side wall of the connection tube (6).
5. A biological cell culture apparatus according to claim 4, wherein the bottom of the protective housing (1) is fixedly provided with a support base.
6. A biological cell incubator as claimed in claim 5, characterised in that the cable (25) is in a relaxed state.
7. The biological cell culture device according to claim 6, wherein the first spring (3) is made of spring steel, a slot is formed in the side wall of the support plate (4), and the carrier (5) is placed in the slot.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
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CN202110254183.XA CN113150983A (en) | 2021-03-09 | 2021-03-09 | Biological cell culture ware |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
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CN202110254183.XA CN113150983A (en) | 2021-03-09 | 2021-03-09 | Biological cell culture ware |
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CN113150983A true CN113150983A (en) | 2021-07-23 |
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CN202110254183.XA Withdrawn CN113150983A (en) | 2021-03-09 | 2021-03-09 | Biological cell culture ware |
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Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN113636178A (en) * | 2021-08-16 | 2021-11-12 | 重庆城市管理职业学院 | Automatic rigging machine of electricity merchant's commodity circulation waybill |
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2021
- 2021-03-09 CN CN202110254183.XA patent/CN113150983A/en not_active Withdrawn
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN113636178A (en) * | 2021-08-16 | 2021-11-12 | 重庆城市管理职业学院 | Automatic rigging machine of electricity merchant's commodity circulation waybill |
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Application publication date: 20210723 |
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