CN113143998A - Application of double-multi-bacterium agent containing eucalyptus multicasts essential oil in preparation of preparation for preventing and treating pet tear stains - Google Patents
Application of double-multi-bacterium agent containing eucalyptus multicasts essential oil in preparation of preparation for preventing and treating pet tear stains Download PDFInfo
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Abstract
The invention discloses application of a double-multi-bacterium agent containing eucalyptus multicasts essential oil in preparation of a preparation for preventing and treating pet tear stains. The double multi-bacterium agent comprises paenibacillus polymyxa bacterium powder and eucalyptus globulus essential oil in a mass ratio of 3-5:4-8, and the paenibacillus polymyxa bacterium powder is prepared by fermenting and drying paenibacillus polymyxa LCP-01 with the preservation number of CGMCC No. 20819. The double-multi-bacterium agent can be used for effectively preventing and treating the tear stains of the pets caused by bacterial or/and fungal infection and low immunity, relieving the animal tear overflow phenomenon, playing the roles of inhibiting bacteria, expelling insects and removing mites, reducing the earmites of the pets, improving the immunity of the organisms of the pets, and having good application prospect.
Description
Technical Field
The invention belongs to the field of pet breeding, and particularly relates to an application of a double-multi-bacterium agent containing eucalyptus multicasts essential oil in preparation of a preparation for preventing and treating tear stains of pets.
Background
The tear stains of the pets are also called as tear overflow, and are a phenomenon of excessive tear flow on the eyes of the pets. The tear mark is generally located below the pet's eyes and is a reddish-brown mark, particularly on pets with light hair colors. The tear stains may be caused by external factors such as trichiasis, large lacrimal glands, eye diseases, eye infection, malnutrition, nasolacrimal duct obstruction, and internal factors such as liver dysfunction, weak immunity, bile acid secretion insufficiency, and porphyrin metabolic abnormality. The tear stains not only affect the color value of the pet, but also affect the health of the pet, so the problem of tear stains appearing in the pet should be considered important.
At present, the problem of the tear stains of pets is mainly treated by external medicines such as nutrition supplementation, external wiping, hair trimming, silver colloid or tear stain powder and the like, a method for effectively and thoroughly solving the tear stains of the pets is not provided, and the tear stains are easy to relapse.
Disclosure of Invention
In order to solve the defects of the prior art, the invention provides application of a double-multi-bacterium agent containing eucalyptus multicasts essential oil in preparing a preparation for preventing and treating tear stains of pets. The double multi-bacterium agent can effectively prevent and treat the tear stains of pets caused by bacterial or/and fungal infection and weak immunity through scientific proportioning.
In order to realize the purpose of the invention, the invention adopts the following technical scheme to realize:
the invention provides application of a double-multi-bacterium agent containing eucalyptus multicasts essential oil in preparation of a preparation for preventing and treating pet tear stains.
Further, the double multi-bacterium agent comprises paenibacillus polymyxa bacterium powder and eucalyptus globulus essential oil.
Further, the mass ratio of the paenibacillus polymyxa powder to the eucalyptus globulus essential oil is 3-5: 4-8.
Further, the bacterium content of the double multi-bacterium agent is not less than 5 multiplied by 108 CFU/g。
Further, the paenibacillus polymyxa powder is prepared by fermenting and drying the paenibacillus polymyxa LCP-01 with the preservation number of CGMCC No. 20819.
Further, the bacteria content of the paenibacillus polymyxa LCP-01 bacteria powder is not less than 5 multiplied by 109 CFU/g。
Further, the pet tear is the pet tear caused by bacterial or/and fungal infection and low immunity.
Further, when the tear stains of pets are treated, the using method of the dual-multi-bacterium agent comprises the following steps: directly orally taking the double multi-bacterium agent for pets, or mixing the double multi-bacterium agent with pet feed or drinking water for use; the dosage is as follows: 10kg and below pet, 4-8g per day; the double multi-bacterium agent is added by 2-3g for 10kg of pet weight, and is continuously used for 5-10 days.
Further, when preventing pet tear stains, the using method of the dual multi-microbial inoculum comprises the following steps: directly orally taking the double multi-bacterium agent for pets, or mixing the double multi-bacterium agent with pet feed or drinking water for use; the dosage is as follows: 1-3g per day; the application method is continuous for 5-20 days.
Further, the use method of the double multi-microbial inoculum comprises the following steps: directly applying the double multi-bacterium agent on the site of pet infected by bacteria and/or fungi for 5-14 days.
Further, the pets include pet dogs and pet cats.
Compared with the prior art, the invention has the following advantages and beneficial effects:
a Paenibacillus polymyxa LCP-01 strain is screened out from soil, has a good antibacterial effect, is prepared into bacterial powder through fermentation and drying, and is scientifically matched with eucalyptus globulus oil to prepare a double-multi microbial inoculum for preventing and treating pet tear stains. The double-multi-bacterium agent has natural components, no toxic or side effect, no harm to the environment, simple use, and can effectively inhibit the reproduction and growth of bacteria and/or fungi, further play a role in inhibiting bacteria, and simultaneously play a role in expelling parasites and removing mites, improve eye or ear diseases of pets caused by bacteria and/or fungal infection and parasites, further reduce the occurrence probability of tear stains and earmites, and achieve the purpose of preventing and treating the tear stains of the pets. In addition, the double-multi-bacterium agent is easy to absorb, and can not bring uncomfortable feeling to pets and influence the normal life of the pets when the double-multi-bacterium agent is taken orally or applied externally, so that the double-multi-bacterium agent has wide application prospect.
Drawings
FIG. 1 is a photograph of a colony of Paenibacillus polymyxa LCP-01.
Fig. 2 shows the effect of a double multi-microbial inoculum containing eucalyptus multicastus essential oil on tear stains in pet cats suffering from california blanca.
FIG. 3 shows the effect of double multi-microbial agents containing eucalyptus multicasts essential oil on tear stains in bulls.
Detailed Description
The technical solution of the present invention will be described in further detail with reference to specific examples. The experimental procedures, in which specific conditions are not noted in the following examples, are generally carried out according to conventional conditions or according to conditions recommended by the manufacturers.
Example 1:
preparation of Paenibacillus polymyxa LCP-01 fermentation liquor
1. Screening and identification of Paenibacillus polymyxa LCP-01
Taking soil as a sample, uniformly mixing the soil and sterile water to prepare 10-1 diluent of the soil, and then respectively preparing 10-2、10-3、10-4、10-5And (4) diluting the solution. Respectively take 10-3、10-4、10-5The diluted solution was spread on nutrient agar medium (beef extract 3.0g, peptone 10.0g, NaCl 5).0g of agar, 20.0g of agar, 1.0L of distilled water and pH 7.0), culturing for 48 h at the constant temperature of 37 ℃, selecting obvious colonies, purifying for multiple times to obtain single colonies, and naming the single colonies as LCP-01.
As shown in figure 1, the bacterial colony of the strain LCP-01 on nutrient agar medium is round, round-like or oval, beige, 3-5 mm in diameter, flat, smooth and dull in surface, opaque, irregular and wavy in edge and semi-transparent in edge.
Extracting genome DNA of the strain LCP-01, using the genome DNA as a template, using a 16S rRNA universal primer to perform PCR amplification, obtaining a 16S rRNA amplification sequence, then sequencing the sequence, performing Blast comparison on the sequence, and displaying the result that the strain LCP-01 and the Paenibacillus polymyxa in a GenBank gene libraryPaenibacillus polymyxaThe homology is highest, so that the strain LCP-01 is judged to be paenibacillus polymyxa.
And (3) performing strain preservation on the screened Paenibacillus polymyxa LCP-01, wherein the preservation unit comprises the following steps: china general microbiological culture Collection center (CGMCC); address: western road No. 1, north west city of township, beijing, institute of microbiology, china academy of sciences; the preservation date is as follows: 09 month and 25 days 2020; paenibacillus polymyxaPaenibacillus polymyxaThe preservation number of (A) is CGMCC No. 20819.
2. Preparation of Paenibacillus polymyxa LCP-01 bacterial powder
Culturing the preserved Paenibacillus polymyxa LCP-01 in a nutrient liquid culture medium (beef extract 3.0g, peptone 10.0g, NaCl 5.0g, distilled water 1.0L, pH 7.0) at 37 ℃ for 36 h, centrifuging at 10000rmp for 10min, discarding supernatant, re-suspending the obtained precipitated thallus in a sterilized low-salt nutrient liquid culture medium (beef extract 3.0g, peptone 10.0g, NaCl 1.0g, distilled water 1.0L, pH is natural), drying the re-suspended fermentation liquor into powder by a vacuum drying method to obtain the Paenibacillus polymyxa LCP-01 powder, and ensuring that the bacteria content in the powder is not less than 5 multiplied by 109 CFU/g。
Preparation of eucalyptus essential oil
Washing Australian multi-bud eucalyptus leaves in a washing tower, putting the Australian multi-bud eucalyptus leaves in a dryer for drying, crushing the Australian multi-bud eucalyptus leaves in a crusher, then respectively putting the crushed Australian multi-bud eucalyptus leaves into a steam distillation kettle, a condenser and an oil-water delayer for separating an oil phase and a water phase, putting the water phase into an ether extractor for separating wastewater, putting liquid containing ether into an ether recovery tower for recovering the ether, adding the recovered ether and oil phase liquid into a solid bed adsorption dryer for further removing water in the oil phase, and finally adding the oil phase liquid into a reduced pressure distillation tower for rectification so as to remove impurity oil, thereby obtaining the multi-bud eucalyptus essential oil. Storing the prepared eucalyptus multicupillata essential oil in a sealed and lightproof container, and keeping away from light. The eucalyptus globulus oil contains 1, 8-cineole (83.8%), beta-cymene (3.43%), limonene (2.5%), alpha-pinene (1.8%), allomyrcene (1.08%), terpinen-4-ol (0.88%), alpha-terpineol (0.58%), eucalyptol (0.54%), P-cymene (0.51%) and the like by chromatographic analysis.
Preparation of double-multi microbial inoculum containing eucalyptus globulus oil
Mixing the prepared Paenibacillus polymyxa LCP-01 powder and Eucalyptus globulus essential oil at a mass ratio of 3:5 by using a mixer uniformly to obtain the double-multi-microbial-agent with the bacterium content of not less than 5 × 108 CFU/g。
When in use, the double multi-microbial inoculum can be orally taken, mixed in pet food or drinking water, and also externally coated. For improving and curing tear stains, the dosage of the composition is 4-8g per day for pets with a dosage of 10kg or less, and the dosage of the composition is increased by 2-3g per 10kg for continuous use for 5-10 days. In order to consolidate the effect or prevent tear stains, 1-3g of the composition can be orally taken by pets every day for 5-20 days.
Example 2
A pet hospital in the Wenjiang district of the Sichuan Chengdu city accepts a 2-year-old cat pet with weight of 2.7 kg and named as a small shellfish. Shellfish have been severely stained with tears, conjunctivitis and ear mites (fig. 2A), and the problems of tears and conjunctivitis have not been improved after a variety of eye drops have been used. The conjunctivitis of the shellfish belongs to bacterial infectious conjunctivitis, and the tear stains are caused by the conjunctivitis, which is diagnosed by veterinarians in pet hospitals.
After diagnosis, the double multi-bacterium preparation is mixed in cat food of the shellfish at the dosage of 4g per day and is taken, and the proper double multi-bacterium preparation is coated outside the ear of the shellfish every day and is used for 7 days, and during the period, the ear and the eyes of the shellfish are kept clean. After 7 days of re-diagnosis (fig. 2B), the earmites of the shellfish were essentially disappeared, the redness and swelling of conjunctiva was reduced, and slight tear marks were observed. After continuing to use according to the previous method for 10 days (fig. 2C), the earmites of the shellfish disappeared completely, there was essentially no sign of redness and swelling of the conjunctiva, and tear stains disappeared essentially. Therefore, the double-multi-bacterium agent not only has the effects of inhibiting bacteria and expelling parasites and improving conjunctivitis and earmite of the pet, but also can improve the tear mark problem caused by eye infection and restore the color value of the pet.
Example 3
A pet clinic in Chongqing receives a live half-bull dog of 2 years old, which weighs 10.6 jin and is named as XT 5. According to the description of the XT5 owner, XT5 always lachrymates, thus leaving two deep tear marks under the eye (fig. 3A), and also using the usual scrubbing products, although with a short effect, the tear mark problem occurs upon cessation. Veterinary in pet clinics has diagnosed that XT5 is a tear mark caused by weak immunity and slight fungal infection of the eyes, and although not problematic, it still needs to be considered important.
After diagnosis is confirmed, the double-multi-microbial-agent is mixed in dog food of XT5 at the dosage of 4g per day and is taken, and meanwhile, proper double-multi-microbial-agent is coated outside the periphery of eyes of the shellfish per day, because the eucalyptus globulus oil in the double-multi-microbial-agent is not greasy and is absorbed quickly, the double-multi-microbial-agent does not bring any eye discomfort to the bulldog and does not influence normal visual objects, the tear mark of XT5 is obviously improved after 7 days of use, and the tear mark of XT5 basically disappears after 5 days of continuous use (fig. 3B). The tear drop problem did not reappear after one week of temporary application of the dual multi-bacterial agents (fig. 3C). However, in order to continue to consolidate the effect, prevent tear stains and fungal infection and improve the immunity of the bullfight dog, veterinarians recommend that the double multi-bacterium agent is continuously used within a fixed period of 5 days in each month, and the dosage is 2 g/d.
Therefore, the double multi-bacterium agent can inhibit the reproduction and growth of fungi, improve the immunity of pet organisms, improve and prevent the occurrence of the tear stain problem, and the tear stain problem cured by the double multi-bacterium agent is not easy to relapse.
The above examples are only intended to illustrate the technical solution of the present invention, but not to limit it; although the present invention has been described in detail with reference to the foregoing embodiments, it will be apparent to those skilled in the art that various changes may be made and equivalents may be substituted for elements thereof; and such modifications or substitutions do not depart from the spirit and scope of the corresponding technical solutions.
Claims (10)
1. An application of a double-multiple microbial inoculum containing eucalyptus multicasts essential oil in preparing a preparation for preventing and treating pet tear stains.
2. The use of the double multi-bacterial agent containing eucalyptus globulus oil of claim 1 for preparing a preparation for preventing and treating pet tear stains, wherein the double multi-bacterial agent comprises paenibacillus polymyxa powder and eucalyptus globulus oil.
3. The use of the double multi-bacterial agent containing eucalyptus multicasts essential oil according to claim 2, for preparing a preparation for preventing and treating pet tear stains, wherein the pet tear stains are pet tear stains caused by bacterial or/and fungal infection and weak immunity.
4. The application of the double multi-bacterium agent containing the eucalyptus globulus oil in the preparation of the agent for preventing and treating the tear stains of the pets according to claim 2, wherein the mass ratio of the paenibacillus polymyxa bacterium powder to the eucalyptus globulus oil is 3-5: 4-8.
5. The application of the double multi-microbial-agent containing eucalyptus globulus oil in the preparation of the preparation for preventing and treating pet tear stains according to claim 4, wherein the paenibacillus polymyxa powder is prepared by fermenting and drying paenibacillus polymyxa LCP-01 with the preservation number of CGMCC No. 20819.
6. The use of the double-multiple microbial agent containing eucalyptus multicasts essential oil according to claim 2, which has a microbial content of not less than 5 x 10, for preparing a preparation for preventing and treating pet tear stains8 CFU/g。
7. The application of the double-multi-bacterium agent containing eucalyptus multicasts essential oil of claim 3 in preparation of a preparation for preventing and treating pet tear stains, wherein the application method of the double-multi-bacterium agent is as follows: directly orally taking the double multi-bacterium agent for pets, or mixing the double multi-bacterium agent with pet feed or drinking water for use; the dosage is as follows: 10kg and below pet, 4-8g per day; the double multi-bacterium agent is added by 2-3g for 10kg of pet weight, and is continuously used for 5-10 days.
8. The application of the double-multi-bacterium agent containing eucalyptus multicasts essential oil of claim 3 in preparation of a preparation for preventing and treating pet tear stains, wherein the application method of the double-multi-bacterium agent is as follows: directly orally taking the double multi-bacterium agent for pets, or mixing the double multi-bacterium agent with pet feed or drinking water for use; the dosage is as follows: 1-3g per day; the application method is continuous for 5-20 days.
9. The use of the double multi-bacterial agent containing eucalyptus multicasts essential oil according to claim 3 for preparing a preparation for preventing and treating tear stains of pets, wherein the double multi-bacterial agent is directly externally coated on the site of pets infected by bacteria or/and fungi for 5-14 days.
10. Use of the dual multi-bacterial agent comprising eucalyptus multicasts essential oil according to claim 2 for the preparation of a formulation for controlling tear stains in pets, wherein the pets comprise pet dogs and pet cats.
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Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN110558324A (en) * | 2019-09-20 | 2019-12-13 | 山东龙昌动物保健品有限公司 | Eucalyptus multicinctus essential oil with insect expelling effect, and composite microbial agent and application thereof |
| CN112280721A (en) * | 2020-11-19 | 2021-01-29 | 山东龙昌动物保健品有限公司 | Paenibacillus polymyxa LCP-01, compound microbial inoculum compounded with bile acid and application thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN110558324A (en) * | 2019-09-20 | 2019-12-13 | 山东龙昌动物保健品有限公司 | Eucalyptus multicinctus essential oil with insect expelling effect, and composite microbial agent and application thereof |
| CN112280721A (en) * | 2020-11-19 | 2021-01-29 | 山东龙昌动物保健品有限公司 | Paenibacillus polymyxa LCP-01, compound microbial inoculum compounded with bile acid and application thereof |
Non-Patent Citations (1)
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| CB02 | Change of applicant information | ||
| CB02 | Change of applicant information |
Address after: No. 9 Yanying North Road, Yanbei Street Office, Qihe County, Dezhou City, Shandong Province, 253000 Applicant after: Shandong Longchang Animal Health Products Co.,Ltd. Address before: 251100 No.1, North Park Road, Qihe Economic Development Zone, Dezhou City, Shandong Province Applicant before: SHANDONG LONGCHANG ANIMAL HEALTH PRODUCT Co.,Ltd. |