CN110558319A - Application of eucalyptus multiceps essential oil and compound microbial inoculum thereof in preparation of anthelmintic for echinoderm animals - Google Patents
Application of eucalyptus multiceps essential oil and compound microbial inoculum thereof in preparation of anthelmintic for echinoderm animals Download PDFInfo
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- CN110558319A CN110558319A CN201910891413.6A CN201910891413A CN110558319A CN 110558319 A CN110558319 A CN 110558319A CN 201910891413 A CN201910891413 A CN 201910891413A CN 110558319 A CN110558319 A CN 110558319A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N27/00—Biocides, pest repellants or attractants, or plant growth regulators containing hydrocarbons
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N31/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic oxygen or sulfur compounds
- A01N31/04—Oxygen or sulfur attached to an aliphatic side-chain of a carbocyclic ring system
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N35/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having two bonds to hetero atoms with at the most one bond to halogen, e.g. aldehyde radical
- A01N35/06—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having two bonds to hetero atoms with at the most one bond to halogen, e.g. aldehyde radical containing keto or thioketo groups as part of a ring, e.g. cyclohexanone, quinone; Derivatives thereof, e.g. ketals
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
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- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
- C11B9/00—Essential oils; Perfumes
- C11B9/02—Recovery or refining of essential oils from raw materials
- C11B9/022—Refining
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- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
- C11B9/00—Essential oils; Perfumes
- C11B9/02—Recovery or refining of essential oils from raw materials
- C11B9/027—Recovery of volatiles by distillation or stripping
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
Abstract
The invention provides eucalyptus multicasts essential oil and application of a compound microbial inoculum thereof in preparing insect repellents for echinoderm animals, wherein the compound microbial inoculum comprises the eucalyptus multicasts essential oil and the microbial content of not less than 5 multiplied by 108 CFU/g of Streptomyces griseus HL-50, wherein the protection of Streptomyces griseus HL-50the preservation number is CGMCC No.18424, and the main effective component of the eucalyptus multicincta essential oil is 1, 8-cineole. The eucalyptus multicastus essential oil compound microbial inoculum is simple to prepare and use, can effectively repel various parasites infected by the echinoderm and inhibit bacteria only by uniformly mixing the compound microbial inoculum with the amount of 0.5-1.5 kg/ton of feed and continuously feeding for 4-7 days, is nontoxic and harmless, is suitable for long-term use of the echinoderm, reduces the breeding cost, and has wide application prospect.
Description
Technical Field
The invention belongs to the technical field of aquaculture, and particularly relates to eucalyptus multiceps essential oil and application of a compound microbial inoculum thereof in preparation of an insect repellent for echinoderm animals.
background
The essential oil is volatile aromatic substances extracted from flowers, leaves, stems, roots or fruits of plants by a steam distillation method, an extrusion method, a cold soaking method or a solvent extraction method, wherein most of the essential oil is lighter than water, and the relative density is generally 0.8-1.17. The essential oil is sensitive to heat and light, so the essential oil needs to be stored in a dark container and a shade place, and the essential oil is mainly used for maintenance products and aromatherapy at present due to the characteristics of special aroma and solubility in organic solvents, but the plant essential oil has various functions, has the effects of resisting bacteria, preventing spasm, promoting growth, promoting cell metabolism and cell regeneration, enhancing immunity, resisting oxidation and the like besides the traditional functions of flavoring, relieving and refreshing, and can even improve the quality of livestock and poultry products. One of the essential oils is wood-based essential oil extracted from wood-based plants, such as sandalwood, cedar, birch, cedar, eaglewood, eucalyptus, etc.
The eucalyptus is a general name of plants in the eucalyptus genus of the myrtaceae family, most of the plants are tall trees, the shrubs are few, and the medicinal value is rich, while the common eucalyptus essential oil in the market is mostly derived from tall trees such as eucalyptus, but the eucalyptus essential oil is light in color and luster, not thick in sense, and not lasting in smell, and finally influences the using effect of the essential oil. The eucalyptus lobular (multi-bud eucalyptus) is distributed in Australia, grows in a very arid sandy land environment, contains more eucalyptol components than general eucalyptus globulus and has higher extraction rate, and essential oil produced by the multi-bud eucalyptus has high content and good quality, is a precious medical raw material, and can be widely used in the fields of antibiosis and insect expelling.
When the aquatic animals are cultured, various parasites and bacteria can be caused due to incomplete pond cleaning, poor water quality management, improper prevention and the like, so that huge loss is directly or indirectly caused to the whole aquaculture industry. In order to maintain the culture benefit, antibacterial pesticides become the just-needed products for aquaculture. However, in order to simply pursue the instant effect, the traditional medicine has the situations of heavy metal exceeding, unstable medicine dosage and the like, so that the stability of water quality is damaged, the health of aquatic animals is damaged, the immunity of the aquatic animals is reduced, the death event can be directly caused seriously, the production benefit is finally reduced, and the potential safety hazard is brought to consumers. Therefore, it is very important to prepare a natural, healthy, safe and bactericidal anthelmintic for aquatic animals.
Disclosure of Invention
In order to overcome the defects and shortcomings in the prior art, the invention provides the eucalyptus multiceps essential oil and the application of the compound microbial inoculum thereof in preparing the insect repellent for the echinoderm, which can achieve the effects of sterilization and insect repelling, do not influence the health of aquatic animals and have extremely high safety.
In order to realize the purpose of the invention, the invention adopts the following technical scheme to realize:
The invention provides application of eucalyptus multicasts essential oil in preparing an insect repellent for echinoderm animals.
Further: the eucalyptus multicastus essential oil is prepared from eucalyptus multicasts leaves and comprises the following components: 1, 8-cineole, beta-cymene, limonene, alpha-pinene, terpinen-4-ol, allomyrcene, alpha-terpineol, P-cymene, diacetone alcohol, beta-pinene, beta-myrcene, alpha-phellandrene, alpha-terpene, gamma-terpene, mucoaldehyde, carvone, phellandrene, 4-chirp alkene, eucalyptene and beta-eucalyptol.
Further: the preparation process of the eucalyptus multicastus essential oil comprises the following steps:
(1) Processing the leaves of the eucalyptus globulus by a washing tower, a dryer and a grinder to obtain crushed leaves of the eucalyptus globulus;
(2) Respectively putting the multi-bud eucalyptus leaves pieces obtained in the step (1) into a steam distillation kettle, a condenser and an oil-water demixer to obtain eucalyptus essential oil crude oil;
(3) And (3) further removing moisture from the crude oil of the eucalyptus multicasts essential oil obtained in the step (2) through a fixed bed adsorption dryer, and then, removing impurity oil in a reduced pressure rectifying tower to obtain the eucalyptus multicasts essential oil.
the invention also provides application of the compound microbial inoculum of the eucalyptus globulus essential oil in preparing an insect repellent for echinoderm animals.
Further: the eucalyptus multicasts essential oil compound microbial inoculum comprises eucalyptus multicasts essential oil and streptomyces griseus HL-50.
Further: the preservation number of the streptomyces griseus HL-50 is CGMCC No. 18424.
Further: the strain content of the streptomyces griseus HL-50 is not less than 5 multiplied by 108 CFU/g。
Further: the echinoderm comprises: sea cucumber, sea urchin, and starfish.
Further: the insect repellent is capable of killing the following parasites: ciliates, flatworms and nematodes.
Further: the application method of the eucalyptus multicasts essential oil compound microbial inoculum comprises the following steps: the eucalyptus bracteata essential oil compound microbial inoculum is uniformly mixed in the edible feed for the echinoderm at the dosage of 0.5-1.5 kg/ton of feed, and the echinoderm is continuously fed for 4-7 days.
Compared with the prior art, the invention has the following advantages and beneficial effects:
(1) The Australian multicaste eucalyptus globulus is used as a raw material, the components are natural, the ingestion and growth of animals are not influenced, no toxicity or side effect is caused, and the culture water quality is not influenced, so that the prepared multicaste eucalyptus globulus essential oil has better quality, has better insect repelling effect, and can continuously play a role for a long time.
(2) The compound microbial inoculum prepared from the eucalyptus essential oil and the streptomyces griseus HL-50 can effectively repel various parasites infected by echinoderm, can be taken orally, and can also be coated and sprayed externally, so that the compound microbial inoculum can effectively inhibit the propagation of harmful floras in animal intestinal tracts and living environments while expelling parasites, reduces bacterial infection, and further improves the growth performance of the echinoderm, is safe to use, does not generate any toxic or side effect, is simple to prepare, is environment-friendly, has a wide application range and a better effect, saves the cultivation cost, and has a wide application prospect.
Drawings
FIG. 1 is a flow chart of a process for preparing eucalyptus multicupus essential oil according to the present invention.
FIG. 2 is a table showing the specific components of eucalyptus multicupillata essential oil according to the present invention.
FIG. 3 is a photograph of a colony of Streptomyces griseus HL-50 isolated according to the present invention on a Gao's synthetic No. one agar medium.
FIG. 4 shows the result of the application of the eucalyptus essential oil complex bacterial preparation to sea cucumbers, wherein FIG. 4A shows the ulceration and parasitic infection in sea cucumbers; FIG. 4B shows the ulceration and parasitic infection of sea cucumber after 4 days of using the eucalyptus globulus essential oil complex microbial inoculum of the invention.
Detailed Description
The technical solution of the present invention will be described in further detail with reference to specific examples.
Example 1
Preparation of eucalyptus essential oil containing multiple leaves
The preparation process flow of the eucalyptus multibladybutton essential oil is shown in figure 1, firstly, leaves of the eucalyptus multibladybutton are placed into a water washing tower to be washed, then the leaves are placed into a dryer to be dried, then the leaves are crushed in a crusher and then respectively enter a steam distillation kettle, a condenser and an oil-water separator to separate an oil phase and a water phase, wherein the water phase enters an ether extractor to separate waste water, liquid containing ether enters an ether recovery tower to recover the ether, the recovered ether and oil phase liquid are added into a solid bed adsorption dryer to further remove water in the oil phase, and finally the oil phase liquid is added into a reduced pressure rectification tower to be rectified to remove impurity oil, so that the eucalyptus multibladybutton essential oil is obtained.
The eucalyptus multicupillata essential oil prepared in this example was stored in a sealed, light-tight container and kept away from light. Subsequently, the eucalyptus multicupillata essential oil was subjected to chromatographic analysis, and the specific components detected were as shown in fig. 2. From the two figures, the component with the largest mass content in the eucalyptus globulus essential oil is 1, 8-cineole (83.8%), which is also the most main effective component in the eucalyptus globulus essential oil of the invention and has obvious antibacterial and insect repellent effects, and other components comprise beta-cymene (3.43%), limonene (2.5%), alpha-pinene (1.8%), allojunene (1.08%), terpinene-4-ol (0.88%), alpha-terpineol (0.58%), eucalyptol (0.54%), P-cymene (0.51%) and the like.
II, screening, separating and identifying streptomyces griseus HL-50
1. Screening of Streptomyces griseus HL-50
Taking soil as a sample, and uniformly mixing the soil and sterile water to prepare the soil 10-1Diluting the solution, and respectively preparing 10-2、10-3、10-4、10-5And (4) diluting the solution. Respectively take 10-3、10-4、10-5Coating the diluent on Gao's synthetic first agar culture medium, culturing at 30 deg.C for 7d, selecting obvious colony, purifying for multiple times to obtain single colony, named HL-50, and storing in Gao's synthetic first agar slant culture medium. The formula of the Gao's synthetic agar medium I is as follows: KNO31g of soluble starch, 20g of soluble starch, K2HPO4 0.5g，MgSO4·7H2O 0.5g，NaCl 0.5g，FeSO40.01g, 20g of agar, 1000mL of distilled water and pH 7.2. As shown in figure 3, the biological characteristics of the bacterial colony of the bacterial strain HL ℃ ~ 50 in the Gao's synthetic agar ℃ ~ I culture medium are that the bacterial colony is circular, has small area, slightly bulges in the middle, is light earthy yellow, is slightly fluffy and opaque on the surface of the bacterial colony, has abundant growth of aerial hyphae and is milky white, the growth temperature of the bacterial strain is 16 ℃ ~ 40 ℃, the optimum growth temperature is 24 ℃ ~ 35 ℃, the growth pH value is 4 ℃ ~ 9, and the optimum growth pH value is 6 ℃ ~ 8.
2. 16S rRNA sequencing of Streptomyces griseus HL-50
Extracting genomic DNA of the strain HL-50, and using the extracted genomic DNA as a template and 16S rRCarrying out PCR amplification by using the NA universal primer, sequencing after obtaining a 16S rRNA amplification sequence, carrying out Blast comparison on the sequence as shown in SEQ ID No.1, and displaying the results of the strain HL-50 and streptomyces griseus in GenBank gene bankStreptomyces griseusThe homology is highest, so the strain HL-50 is judged to be streptomyces griseus.
And (3) performing strain preservation on the screened streptomyces griseus HL-50, wherein the preservation unit comprises the following steps: china general microbiological culture Collection center (CGMCC); address: western road No.1, north west city of township, beijing, institute of microbiology, china academy of sciences; the preservation date is as follows: 8 month 27 year 2019; streptomyces griseusStreptomyces griseusThe preservation number of (2) is CGMCC No. 18424.
Preparation of multi-bud eucalyptus essential oil compound microbial inoculum
The content of the eucalyptus globulus oil and bacteria is not less than 5 × 108 And mixing the CFU/g streptomyces griseus HL-50 uniformly to prepare the eucalyptus essential oil composite microbial inoculum. When the eucalyptus globulus bracteatum essential oil compound microbial inoculum is used, the compound microbial inoculum is mixed in the edible feed of the echinoderm according to the dosage of 0.5-1.5 kg/ton feed, and the feeding is carried out according to the normal feeding management for 4-7 days continuously.
example 2
The eucalyptus multicasts essential oil compound fungicide prepared in the example 1 is subjected to an insect repelling experiment, the test site is selected to cultivate 2000 cubic meters of sea cucumber in the Ling sea of Liaoning, each cement pond is 25 cubic meters, and the cultivation specification is 1000-1500 heads/jin. After fester is found on the surface of the sea cucumber and parasites are detected, 1 kg of eucalyptus globulus essential oil compound microbial inoculum is mixed with 1 ton of feed according to normal feeding management, and the feed is continuously fed for 4 days and is fed for 3 times every day. After 4 days, the sea cucumber was again examined for the presence of parasites. As shown in figure 4, in the initial feeding stage, the body surface of the sea cucumber is ulcerated, and ciliates are found at the wound; after feeding for 4 days, the festered wound of the sea cucumber is detected to be healed and the wound has no ciliate, which shows that the eucalyptus globulus oil compound microbial inoculum has obvious insect expelling effect on the sea cucumber.
Example 3
The eucalyptus globulus oil prepared by the method is subjected to an insect expelling experiment, and the test site is selected in a marigold sea urchin culture base. Ciliates are found in body fluid of sea urchins, 1 kg of eucalyptus multicupillata essential oil is mixed with 1 ton of feed, and sea urchins are continuously fed according to normal feeding management for 3 times per day. After 7 days, the parasite condition of sea urchin is checked again, and the sea urchin body fluid is found to be free of ciliates, which shows that the eucalyptus multicupillata essential oil has obvious insect expelling effect on sea urchin.
The above examples are only intended to illustrate the technical solution of the present invention, but not to limit it; although the present invention has been described in detail with reference to the foregoing embodiments, it will be apparent to those skilled in the art that various changes may be made and equivalents may be substituted for elements thereof; and such modifications or substitutions do not depart from the spirit and scope of the corresponding technical solutions.
Sequence listing
<110> Shandong Longchang animal health products Co Ltd
<120> application of eucalyptus multiceps essential oil and compound bacterium agent thereof in preparation of anthelmintic for echinoderm
<160> 1
<170> SIPOSequenceListing 1.0
<210> 1
<211> 1475
<212> DNA
<213> Streptomyces griseus
<400> 1
acgaacgctg gcggcgtgct taacacatgc aagtcgaacg atgaagcctt tcggggtgga 60
ttagtggcga acgggtgagt aacacgtggg caatctgccc ttcactctgg gacaagccct 120
ggaaacgggg tctaataccg gataacactc tgtcccgcat gggacggggt taaaagctcc 180
ggcggtgaag gatgagcccg cggcctatca gcttgttggt ggggtaatgg cctaccaagg 240
cgacgacggg tagccggcct gagagggcga ccggccacac tgggactgag acacggccca 300
gactcctacg ggaggcagca gtggggaata ttgcacaatg ggcgaaagcc tgatgcagcg 360
acgccgcgtg agggatgacg gccttcgggt tgtaaacctc tttcagcagg gaagaagcga 420
gagtgacggt acctgcagaa gaagcgccgg ctaactacgt gccagcagcc gcggtaatac 480
gtagggcgca agcgttgtcc ggaattattg ggcgtaaaga gctcgtaggc ggcttgtcac 540
gtcggatgtg aaagcccggg gcttaacccc gggtctgcat tcgatacggg ctagctagag 600
tgtggtaggg gagatcggaa ttcctggtgt agcggtgaaa tgcgcagata tcaggaggaa 660
caccggtggc gaaggcggat ctctgggcca ttactgacgc tgaggagcga aagcgtgggg 720
agcgaacagg attagatacc ctggtagtcc acgccgtaaa cgttgggaac taggtgttgg 780
cgacattcca cgtcgtcggt gccgcagcta acgcattaag ttccccgcct ggggagtacg 840
gccgcaaggc taaaactcaa aggaattgac gggggcccgc acaagcagcg gagcatgtgg 900
cttaattcga cgcaacgcga agaaccttac caaggcttga catataccgg aaagcatcag 960
agatggtgcc ccccttgtgg tcggtataca ggtggtgcat ggctgtcgtc agctcgtgtc 1020
gtgagatgtt gggttaagtc ccgcaacgag cgcaaccctt gttctgtgtt gccagcatgc 1080
ccttcggggt gatggggact cacaggagac tgccggggtc aactcggagg aaggtgggga 1140
cgacgtcaag tcatcatgcc ccttatgtct tgggctgcac acgtgctaca atggccggta 1200
caatgagctg cgatgccgcg aggcggagcg aatctcaaaa agccggtctc agttcggatt 1260
ggggtctgca actcgacccc atgaagtcgg agttgctagt aatcgcagat cagcattgct 1320
gcggtgaata cgttcccggg ccttgtacac accgcccgtc acgtcacgaa agtcggtaac 1380
acccgaagcc ggtggcccaa ccccttgtgg gagggagctg tcgaaggtgg gactggcgat 1440
tgggacgaag tcgtaacaag gtagccgtac cggaa 1475
Claims (10)
1. An application of eucalyptus multicasts essential oil in preparing anthelmintic for Acanthopanax animals is provided.
2. use of eucalyptus multicastus essential oil according to claim 1 for the preparation of an insect repellent for animals of the echinoderm group, characterized in that: the eucalyptus multicastus essential oil is prepared from eucalyptus multicasts leaves and comprises the following components: 1, 8-cineole, beta-cymene, limonene, alpha-pinene, terpinen-4-ol, allomyrcene, alpha-terpineol, P-cymene, diacetone alcohol, beta-pinene, beta-myrcene, alpha-phellandrene, alpha-terpene, gamma-terpene, mucoaldehyde, carvone, phellandrene, 4-chirp alkene, eucalyptene and beta-eucalyptol.
3. Use of eucalyptus multicastus essential oil according to claim 1 or 2 for the preparation of an insect repellent for animals of the echinoderm group, characterized in that: the preparation process of the eucalyptus multicastus essential oil comprises the following steps:
(1) Processing the leaves of the eucalyptus globulus by a washing tower, a dryer and a grinder to obtain crushed leaves of the eucalyptus globulus;
(2) Respectively putting the multi-bud eucalyptus leaves pieces obtained in the step (1) into a steam distillation kettle, a condenser and an oil-water demixer to obtain eucalyptus essential oil crude oil;
(3) And (3) further removing moisture from the crude oil of the eucalyptus multicasts essential oil obtained in the step (2) through a fixed bed adsorption dryer, and then, removing impurity oil in a reduced pressure rectifying tower to obtain the eucalyptus multicasts essential oil.
4. Use of a complex microbial agent containing eucalyptus globulus oil of claim 1 for preparing an insect repellent for echinoderm.
5. The use of the eucalyptus multibuisticida essential oil complex microbial agent for preparing an insect repellent for echinoderm, according to claim 4, wherein: the eucalyptus multicasts essential oil compound microbial inoculum comprises eucalyptus multicasts essential oil and streptomyces griseus HL-50.
6. The application of the eucalyptus bracteata essential oil composite microbial inoculum for preparing the anthelmintic for the echinoderm as claimed in claim 5, wherein the preservation number of the streptomyces griseus HL-50 is CGMCC No. 18424.
7. The application of the eucalyptus multibuisiana essential oil composite microbial agent for preparing the insect repellent for the echinoderm as claimed in claim 6, wherein the bacterial content of the streptomyces griseus HL-50 is not less than 5 x 108 CFU/g。
8. Use of the eucalyptus multicastus essential oil of claim 1 or the composite microbial agent of claim 3 for preparing an insect repellent for echinoderm, characterized in that: the echinoderm comprises: sea cucumber, sea urchin, and starfish.
9. Use according to claim 8, characterized in that: the insect repellent is capable of killing the following parasites: ciliates, flatworms and nematodes.
10. Use according to claim 8, characterized in that: the application method of the eucalyptus multicasts essential oil compound microbial inoculum comprises the following steps: the eucalyptus bracteata essential oil compound microbial inoculum is uniformly mixed in the edible feed for the echinoderm at the dosage of 0.5-1.5 kg/ton of feed, and the echinoderm is continuously fed for 4-7 days.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113018243A (en) * | 2021-02-03 | 2021-06-25 | 山东龙昌动物保健品有限公司 | Application of double-multi-bacterium agent containing eucalyptus multicasts essential oil in preparation of preparation for removing pet odor |
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| CN101420848A (en) * | 2006-02-15 | 2009-04-29 | 博塔诺凯普有限公司 | Applications of microencapsulated essential oils |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN113018243A (en) * | 2021-02-03 | 2021-06-25 | 山东龙昌动物保健品有限公司 | Application of double-multi-bacterium agent containing eucalyptus multicasts essential oil in preparation of preparation for removing pet odor |
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Address after: No. 9 Yanying North Road, Yanbei Street Office, Qihe County, Dezhou City, Shandong Province, 253000 Patentee after: Shandong Longchang Animal Health Products Co.,Ltd. Address before: 251100 No.1, North Park Road, Qihe Economic Development Zone, Dezhou City, Shandong Province Patentee before: SHANDONG LONGCHANG ANIMAL HEALTH PRODUCT Co.,Ltd. |