CN113142212B - Millet compound seed dressing agent with disease resistance, growth promotion and yield increase functions and preparation method thereof - Google Patents

Millet compound seed dressing agent with disease resistance, growth promotion and yield increase functions and preparation method thereof Download PDF

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CN113142212B
CN113142212B CN202110315456.7A CN202110315456A CN113142212B CN 113142212 B CN113142212 B CN 113142212B CN 202110315456 A CN202110315456 A CN 202110315456A CN 113142212 B CN113142212 B CN 113142212B
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millet
seed dressing
dressing agent
metalaxyl
yield increase
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CN113142212A (en
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李君霞
王春义
段俊枝
朱灿灿
秦娜
代书桃
宋迎辉
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Institute Of Grain Crop Henan Academy Of Agricultural Sciences
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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
    • A01N37/44—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a nitrogen atom attached to the same carbon skeleton by a single or double bond, this nitrogen atom not being a member of a derivative or of a thio analogue of a carboxylic group, e.g. amino-carboxylic acids
    • A01N37/46—N-acyl derivatives
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01C—PLANTING; SOWING; FERTILISING
    • A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
    • A01C1/06—Coating or dressing seed
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01C—PLANTING; SOWING; FERTILISING
    • A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
    • A01C1/08—Immunising seed
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/34—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom
    • A01N43/36—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom five-membered rings
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N57/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic phosphorus compounds
    • A01N57/10—Biocides, pest repellants or attractants, or plant growth regulators containing organic phosphorus compounds having phosphorus-to-oxygen bonds or phosphorus-to-sulfur bonds
    • A01N57/14—Biocides, pest repellants or attractants, or plant growth regulators containing organic phosphorus compounds having phosphorus-to-oxygen bonds or phosphorus-to-sulfur bonds containing aromatic radicals
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N59/00—Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
    • A01N59/26—Phosphorus; Compounds thereof

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  • Environmental Sciences (AREA)
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  • Dentistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Pest Control & Pesticides (AREA)
  • Plant Pathology (AREA)
  • Agronomy & Crop Science (AREA)
  • Soil Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Inorganic Chemistry (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Pretreatment Of Seeds And Plants (AREA)

Abstract

The invention discloses a millet composite seed dressing agent with disease resistance, growth promotion and yield increase and a preparation method thereof, and aims to solve the technical problems that the current millet seed dressing control effect is single and the millet growth and development are easily adversely affected. The main effective components of the millet composite seed dressing agent are metalaxyl-M, phoxim, fludioxonil and potassium dihydrogen phosphate, and the weight ratio of the metalaxyl-M to the potassium dihydrogen phosphate is 100-110: 115-125: 0.10-0.15: 140-300 by mass. The compound seed dressing agent for millet integrates sterilization, disinsection and nutrition, can prevent and treat main diseases and insect pests of millet, promotes the emergence of seedlings and strengthens the seedlings of the millet, and finally realizes yield increase.

Description

Millet compound seed dressing agent with disease resistance, growth promotion and yield increase functions and preparation method thereof
Technical Field
The invention relates to the technical field of grain planting, in particular to a millet compound seed dressing agent with disease resistance, growth promotion and yield increase and a preparation method thereof.
Background
Millet (A), (B)Setaria italica) A plant belonging to the family Gramineae is called millet, millet and sorghum. Millet is an ancient crop in China and historically extends all over the country. The poetry sentence of "large mice, no broomcorn millet" in the "shi jing & guo feng" reflects the fact that millet is used as staple food at that time.
The grains have high nutritive value, and are rich in protein, fat, vitamins, and mineral components such as calcium, iron, selenium, etc. The millet is not only edible, but also has the effects of clearing heat, quenching thirst, nourishing yin, tonifying spleen, kidney, intestines and stomach, promoting urination, treating watery diarrhea and the like when being used as a medicine, and can be used for brewing wine. It has effects of lowering blood pressure, preventing and treating dyspepsia, replenishing blood, nourishing brain, improving sleep, reducing wrinkle, mottle, and pigmentation, and caring skin.
At present, millet is one of important coarse cereal crops in northern China, and with the improvement of life quality of people, the planting area of millet is increased to some extent in recent years, but the control of diseases is not yet taken into consideration. During the growth process of the millet, the risk of being invaded by various germs exists, and millet diseases and insect pests not only are one of important factors causing the yield reduction of the millet, but also influence the yield and the quality of the millet. How to control the diseases and insect pests of millet is an urgent problem to be solved. The white disease and the nematode disease are important diseases in the summer valley area at present, and the incidence of the banded sclerotial blight in the west Henan valley planting area is also in an increasing trend. The selection of disease-resistant varieties and a reasonable farming mode can effectively prevent and treat the millet diseases. However, the related research discovers that the current main production varieties are susceptible varieties to the white-sickness disease, the nematode disease and the sheath blight disease through the primary identification of the resistance of the current main production varieties in each production area. Therefore, the key link of mixing before sowing must be grasped.
In recent years, the disease and insect pest control of the millet is reported more through medicament seed dressing, but most of the medicament seed dressing is a single finished product medicament, and the medicament seed dressing also has adverse effects on seedling germination, growth and development of the millet.
Disclosure of Invention
The invention aims to provide a millet compound seed dressing agent with disease resistance, growth promotion and yield increase and a preparation method thereof, and aims to solve the technical problems that the current millet seed dressing control effect is single and adverse effects are easily caused on the seedling germination and growth development of millet.
In order to solve the technical problems, the invention adopts the following technical scheme:
the millet compound seed dressing agent with disease resistance, growth promotion and yield increase is designed, and the main effective components of the millet compound seed dressing agent are metalaxyl-M, phoxim, fludioxonil and potassium dihydrogen phosphate, wherein the weight ratio of the metalaxyl-M to the potassium dihydrogen phosphate is (100-110): 115-125: 0.10-0.15: 140-300 by mass.
The millet compound seed dressing agent is prepared from the following raw materials in 1L:
286-314 ml of 350g/L metalaxyl-M emulsion, 288-310 ml of 400g/L phoxim missible oil, 400-600 ml of 25g/L fludioxonil suspending agent and KH2PO4140-300 g and the balance of water.
The preparation method of the millet composite seed dressing agent comprises the following steps:
(1) preparing metalaxyl-M emulsion, phoxim missible oil, fludioxonil suspending agent and potassium dihydrogen phosphate according to the effective component proportion;
(2) firstly, dissolving monopotassium phosphate in water to prepare a solution, sequentially adding fludioxonil suspending agent and metalaxyl-M emulsion, and uniformly stirring;
(3) then adding the phoxim missible oil, stirring uniformly, and adding water to adjust to the required concentration.
The preparation method of the millet composite seed dressing agent comprises the following steps:
(1) 1L of the pesticide is prepared with 286-314 ml of 350g/L metalaxyl-M emulsion, 288-310 ml of 400g/L phoxim missible oil, 400-600 ml of 25g/L fludioxonil suspending agent and KH2PO4 140～300g；
(2) First KH is added2PO4Dissolving in water to prepare a solution, sequentially adding fludioxonil suspending agent and metalaxyl-M emulsion, and uniformly stirring;
(3) then adding the phoxim missible oil, stirring uniformly, adding water to 1L, and mixing uniformly to obtain the finished product.
Compared with the prior art, the invention has the main beneficial technical effects that:
the compound millet seed dressing agent integrates sterilization, disinsection and nutrition, can prevent and control main diseases and pests (such as banded sclerotial blight, leukotrichia, nematode disease and the like) of the millet, promotes the germination of seeds and the growth of root systems, improves the emergence rate and stress resistance, increases the weight of ears and the weight of grains of the ears, and finally realizes yield increase.
Drawings
FIG. 1 is a graph comparing the effect of different dressing treatments on millet germination.
Detailed Description
The following examples are given to illustrate specific embodiments of the present invention, but are not intended to limit the scope of the present invention in any way.
The following species and reagents are referred to in the examples below:
the test varieties are: yu Gu 18 and Yu Gu 29.
Test reagents: the 350g/L metalaxyl-M seed treatment emulsion (Jinaprlong, Zhang Mingda crop protection Co., Ltd.) is a systemic aniline compound, and has very good prevention effect on various seed-borne and soil-borne diseases caused by low-grade fungi of oomycetes such as pythium, cotton mold and the like; the 25g/L fludioxonil suspension seed coating agent (Zygun Zhengzhengda crop protection Limited company, if applicable) is a non-systemic phenylpyrrole compound, and has very good control effect on seed-borne and soil-borne diseases caused by many pathogenic bacteria such as ascomycetes, basidiomycetes, deuteromycetes and the like; 400g/L Phoxim emulsifiable concentrate (Shandong Esson chemical Co., Ltd.); 200g/kg gibberellin wettable powder (Chinese chemical).
Other materials or methods not specifically mentioned are conventional materials or methods.
The first embodiment is as follows: application effect verification of millet composite seed dressing agent with disease resistance, growth promotion and yield increase functions
1. Dressing treatment
6 different seed dressing agent compounding schemes (shown in table 1) are designed for effect comparison, and the mixed seed dressing agent is added with clear water to be diluted to 10ml and fully stirred with 1kg of carefully chosen seeds and dried; meanwhile, the seeds are dressed with 10ml of clear water as a control.
Table 1 different seed dressing agent compounding test treatments
Figure 560375DEST_PATH_IMAGE001
。
2. Indoor germination and cultivation
Each seed-dressing treated millet seed was placed in a petri dish on which filter paper was laid, and 10ml of distilled water was added. Placing in a constant temperature incubator in the dark at 25 ℃ for germination, and repeating for 3 times when 100 seeds are treated. The filter paper was changed every 3 days 1 time. The germination was recorded 1 time every 2 days, with the radicle breaking 0.5cm as the standard until day 8. And the germination vigor was investigated on day 4 and the germination rate was investigated on day 8, 5 whole millet seedlings were taken, rinsed with water and carefully straightened, and the bud length and root length of the seedlings were measured with a ruler.
Germination percentage (%) = N0/N × 100%; in the formula, N0 is the number of all germinated seeds in the final germination period; n is the number of test seeds.
Germination potential (%) = N4/N × 100%; wherein N4 is the number of normal germinated seeds on the 4 th day of seed germination; n is the number of test seeds.
The test results are shown in tables 2 and 3.
(1) Influence of compound seed dressing agent on millet germination
As can be seen from Table 2, the germination vigor of the Yugu 29 after the seed dressing treatment is shown as T2> T5> CK > T3 > T6> T4> T1, and the germination rate is shown as T2> T5> T6> CK > T3 > T1 > T4. The germination potential of the Yu-Gu 18 is represented by T5> T6> T2> CK > T3 > T1 > T4, and the germination rate is represented by T5> T2> T6> CK > T3 > T4> T1.
From the analysis results, the germination vigor and the germination rate of the T1 and the T4 which are only used for dressing the seeds by the pesticides metalaxyl-M, phoxim and fludioxonil are lower than those of CK, which indicates that the germination rate of the seeds is reduced due to the pesticide injury, mechanical damage and the like in the seed dressing process.
As can be seen from FIG. 1, the GA is added3And CaCl2Then, the low-dose treatment of T2 improves the germination vigor and the germination rate of the seeds compared with T1 and CK; while the high dose treatment T3 compares with T1The germination potential and the germination rate of the seeds are improved, but both the germination potential and the germination rate do not reach the CK level.
Adding KH2PO4Then, the low-dose treatment of T5 improves the germination potential and the germination rate of the seeds compared with T4, and the germination potential and the germination rate both exceed CK; compared with T4, the high-dose treatment of T6 improves the germination potential and the germination rate of seeds, the germination rate of Yugu 29 exceeds CK, and the germination potential and the germination rate of Yugu 18 both exceed CK.
TABLE 2 influence of the Compound seed-dressing agent on the germination vigor and germination rate of millet
Figure DEST_PATH_IMAGE002
[ Note ] the new replicate-difference test, 5% significant difference level, the following table is the same.
(2) Influence of compound seed dressing agent on growth of millet seedlings
TABLE 3 Effect of Compound seed dressing on millet seedling growth
Figure 211937DEST_PATH_IMAGE003
。
As can be seen from Table 3, the seedling length of Yugu 29 is represented by T5> T2> T3 > CK > T6> T4> T1, the fresh weight of seedlings is represented by T5> T2> T6> CK > T3 > T4> T1, the root length is represented by T5> T6> T2> T3 > CK > T4> T1, and the fresh weight of roots is represented by T5> T6> T2> CK > T3 > T4> T1. The seedling length of Yugu 18 is represented by T2> T5> T3 > CK > T6> T1 > T4, the fresh weight of the seedlings is represented by T5> T2> T6> CK > T4> T3 > T1, the root length is represented by T5> T6> T2> CK > T3 > T4> T1, and the fresh weight of the roots is represented by T5> T6> T2> CK > T3 > T1 > T4.
The T1 and T4 treated seedlings have weak growth vigor, and the seedling length, the fresh weight of the seedlings, the root length and the fresh weight of the roots are all lower than CK, which indicates that the seed dressing agent can inhibit the growth of the seedlings in the seed germination process.
Adding KH2PO4After thatCompared with T4 and CK, the low-dose treatment of T5 improves the seedling length, the fresh weight, the root length and the fresh weight of the roots of the seedlings; compared with T4, the high-dose treatment of T6 improves the seedling length, the seedling fresh weight, the root length and the root fresh weight, and the seedling fresh weight, the root length and the root fresh weight all exceed CK.
3. Investigation of field pest occurrence condition and yield traits
Sowing the seeds after seed dressing treatment in the test field of modern agricultural research and development base in Henan province at 20 days 6 months in 2020 for field test, wherein the sowing amount is 0.5kg/666.7m2Line spacing of 0.4m, line length of 5m, 10 lines per cell, cell area of 20m2Artificial thinning in 3-4 leaf stage and final thinning in 5-6 leaf stage. The density of the seedlings remained is 4.0 ten thousand plants/666.7 m2The field test adopts random block arrangement and 3 times of repetition.
The pest control is not carried out in the whole growth period of each cell; and weeding, watering, topdressing and other field management are carried out in time. The plant height, ear length, ear thickness, ear weight, ear grain weight, etc. were investigated at maturity. The test is carried out on 22 days 9 months in 2020, and two side rows (1 row on each side) are removed during harvesting, and 8 rows in the middle are harvested for yield measurement.
The investigation of the occurrence condition of the field diseases and insect pests is carried out according to the following standards:
sheath blight: in the middle and later period of grouting, the investigation was divided into five grades, which are respectively represented by 0, 1, 2, 3 and 4.
Level 0: no disease symptoms (high resistance);
level 1: 1-2 leaf sheaths of the main stem part have ring-shaped scab (anti);
and 2, stage: the upper part of the main stem is provided with 3-5 leaf sheaths with ring-shaped scab (anti-resistance);
and 3, level: the scabs of the ring striation are formed on the leaf sheaths of more than 6 leaves on the overground part of the main stem;
4, level: the leaf sheath of the whole plant shows striated lesions (severe infection).
White disease, nematode disease: all are expressed as the disease incidence.
The disease rate (%) = number of diseased stems ÷ number of sampled stems × 100.
The test results are shown in tables 4 and 5.
(1) Influence of compound seed dressing agent on occurrence of field diseases and insect pests of millet
TABLE 4 influence of the Compound seed-dressing agent on the occurrence of diseases and pests in millet field
Figure DEST_PATH_IMAGE004
。
As can be seen from Table 4, after the seed dressing of the compound seed dressing agent, the incidence of the white disease and the nematode disease of 6 treatments is lower than that of the control, and because the local banded sclerotial blight is less, the incidence of each treatment and CK is not caused.
(2) Influence of compound seed dressing agent on millet yield and related characters
TABLE 5 Effect of Compound seed dressings on millet yield and related traits
Figure 663778DEST_PATH_IMAGE005
。
As can be seen from Table 5, the yields of the 6 treatments are all higher than CK, and the T2, T3 and T5 treatments of Yugu 29 increase the yield significantly than CK, wherein the yield of T5 is the highest, and the T1, T2, T3, T5 and T6 treatments of Yugu 18 increase the yield significantly than CK, wherein the yield of T5 is the highest.
The panicle weight of the yugu 29 is represented by T2> T5> T6> T4> T3 > T1 > CK, wherein T2, T4, T5 and T6 are significantly greater than CK; the panicle weight of the valley 18 is represented by T5> T2> T6> T4> T1 > T3 > CK, wherein T5 is significantly greater than CK. The panicle weight of the yu valley 29 is represented by T2> T5> T6> T4> T3 > T1 > CK, wherein T5 and T2 are significantly greater than CK; the panicle weight of the yu valley 18 is represented by T5> T2> T4> T6> T1 > T3 > CK, wherein T5, T2, T4, T6 and T1 are all significantly greater than CK.
The results of indoor germination tests and field tests are integrated, the germination vigor, the germination rate, the ear weight and the ear grain weight of the T2 treatment of Yugu 29 are the largest, and the yield, the seedling length, the seedling fresh weight, the root length and the root fresh weight of the T5 treatment are the largest; the T2 treated seedlings of Yugu 18 have the longest length, and the T5 treated seedlings have the largest yield, germination vigor, germination rate, seedling fresh weight, root length, root fresh weight, spike weight and spike grain weight.
The test results show that the material has the characteristics of,the seed dressing agent can enhance the disease resistance of the millet, increase the weight of the ear and the weight of the grain of the ear and improve the yield of the millet, but the T1 and T4 which only use pesticides of metalaxyl-M, phoxim and fludioxonil for dressing seeds have influence on the germination vigor and the germination rate of the seeds due to mechanical damage, medicament stimulation on seed coats and the like. Treatment of T5, T6 and T2 with addition of an appropriate concentration of GA3+CaCl2And KH2PO4The germination vigor and the germination rate of seeds can be improved, the growth of seedlings is promoted, and the T5 effect is the best.
While the present invention has been described in detail with reference to the embodiments, it will be understood by those skilled in the art that various changes in the specific parameters of the embodiments and/or substitutions of related steps, methods and reagents may be made without departing from the spirit of the invention, and these embodiments are not intended to limit the scope of the invention.

Claims (4)

1. The compound millet seed dressing agent with the functions of disease resistance, growth promotion and yield increase is characterized in that the effective components of the compound millet seed dressing agent are metalaxyl-M, phoxim, fludioxonil and potassium dihydrogen phosphate, and the effective components are as follows: 115-125: 0.10-0.15: 140-300 by mass.
2. The millet compound seed dressing agent with the functions of disease resistance, growth promotion and yield increase according to claim 1, which is characterized by being prepared from the following raw materials in 1L:
286-314 ml of 350g/L metalaxyl-M emulsion, 288-310 ml of 400g/L phoxim missible oil, 400-600 ml of 25g/L fludioxonil suspending agent and KH2PO4140-300 g and the balance of water.
3. The preparation method of the millet compound seed dressing agent with the functions of disease resistance, growth promotion and yield increase as claimed in claim 1, which comprises the following steps:
(1) the metalaxyl-M emulsion, phoxim missible oil, fludioxonil suspending agent and potassium dihydrogen phosphate are prepared according to the effective component proportion of claim 1;
(2) firstly, dissolving monopotassium phosphate in water to prepare a solution, sequentially adding fludioxonil suspending agent and metalaxyl-M emulsion, and uniformly stirring;
(3) then adding the phoxim missible oil, stirring uniformly, and adding water to adjust to the required concentration.
4. The preparation method of the millet compound seed dressing agent with the functions of disease resistance, growth promotion and yield increase according to claim 3, comprising the following steps:
(1) 1L of the pesticide is prepared with 286-314 ml of 350g/L metalaxyl-M emulsion, 288-310 ml of 400g/L phoxim missible oil, 400-600 ml of 25g/L fludioxonil suspending agent and KH2PO4 140～300g；
(2) First KH is added2PO4Dissolving in water to prepare a solution, sequentially adding fludioxonil suspending agent and metalaxyl-M emulsion, and uniformly stirring;
(3) then adding the phoxim missible oil, stirring uniformly, adding water to 1L, and mixing uniformly to obtain the finished product.
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