CN113125188A - Full-automatic nucleic acid extraction instrument for fecal samples - Google Patents
Full-automatic nucleic acid extraction instrument for fecal samples Download PDFInfo
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- CN113125188A CN113125188A CN202110426389.6A CN202110426389A CN113125188A CN 113125188 A CN113125188 A CN 113125188A CN 202110426389 A CN202110426389 A CN 202110426389A CN 113125188 A CN113125188 A CN 113125188A
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/02—Devices for withdrawing samples
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M1/00—Apparatus for enzymology or microbiology
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/00584—Control arrangements for automatic analysers
- G01N35/00722—Communications; Identification
- G01N35/00732—Identification of carriers, materials or components in automatic analysers
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/02—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations
- G01N35/04—Details of the conveyor system
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/00584—Control arrangements for automatic analysers
- G01N35/00722—Communications; Identification
- G01N35/00732—Identification of carriers, materials or components in automatic analysers
- G01N2035/00821—Identification of carriers, materials or components in automatic analysers nature of coded information
- G01N2035/00831—Identification of carriers, materials or components in automatic analysers nature of coded information identification of the sample, e.g. patient identity, place of sampling
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/02—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations
- G01N35/04—Details of the conveyor system
- G01N2035/0439—Rotary sample carriers, i.e. carousels
- G01N2035/0446—Combinations of the above
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Abstract
The invention relates to a full-automatic nucleic acid extraction instrument for a stool sample, which comprises a container receiving assembly, a visual sampling assembly and a nucleic acid extraction instrument, wherein the container receiving assembly is connected with the visual sampling assembly; the container receiving assembly comprises a container positioning jig, a sealing unit and a conveying belt unit; the visual sampling assembly comprises a scanning unit, a visual identification unit, a sampling spoon, a mobile unit, a sample storage test tube and a control host; the control host receives and identifies the image data of the visual identification unit, compares the image data with sample data in a sampling database to determine a sampling point, controls the mobile unit to drive the sampling spoon to move to the sampling point for sampling, and places the sampling spoon into a sample storage test tube corresponding to a sampling code on a container for storage; the nucleic acid extractor is used for receiving the sample storage test tube and extracting nucleic acid from the internal sample; degree of automation is high, and manual intervention is less, and the treatment effeciency is high, and sample smell loss is less, and the accuracy and the reliability of sample are promoted by a wide margin simultaneously, the sample condition of making mistakes also can not appear.
Description
Technical Field
The invention relates to the technical field of fecal sample treatment, in particular to a fecal sample full-automatic nucleic acid extraction instrument.
Background
The fecal sample is an important one of human nucleic acid detection samples, and can directly or indirectly diagnose various diseases through the nucleic acid detection of microorganisms in the feces;
at present, when carrying out nucleic acid extraction to the excrement and urine sample, all carry out the sample operation by the people usually, carry out nucleic acid extraction by the nucleic acid extraction appearance again, manual operation work load is big, because of there is the stink in the sample, and operational environment is very abominable, and manual operation still can have unstable factors such as mistake, needs one kind can be used to the full-automatic nucleic acid extraction instrument of excrement and urine sample to solve this problem urgently.
Disclosure of Invention
The invention aims to solve the technical problem of providing a full-automatic nucleic acid extraction instrument for a fecal sample, aiming at the defects in the prior art.
The technical scheme adopted by the invention for solving the technical problems is as follows:
constructing a full-automatic nucleic acid extraction instrument for a stool sample, wherein the instrument comprises a container receiving assembly, a visual sampling assembly and a nucleic acid extraction instrument; the container receiving assembly comprises a container positioning jig, a sealing unit for sealing the container by adopting a transparent film, and a conveying belt unit for conveying the sealed container to a waiting station of the visual sampling assembly; the visual sampling assembly comprises a scanning unit for reading a bar code on the container, a visual identification unit for photographing a sample in the container through a transparent film, a sampling spoon which penetrates through the transparent film and extends into the container for sampling, a moving unit for clamping and driving the sampling spoon to move, a sample storage test tube and a control host; the control host receives and identifies the image data of the visual identification unit, compares the image data with sample data in a sampling database to determine a sampling point, controls the mobile unit to drive the sampling spoon to move to the sampling point for sampling and places the sampling spoon into a sample storage test tube corresponding to a sampling code on a container for storage; the nucleic acid extractor is used for receiving the sample storage test tube and extracting nucleic acid from the internal sample.
The invention relates to a full-automatic nucleic acid extraction instrument for a fecal sample, wherein a sampling spoon comprises a spoon handle and a spoon head which are connected; the spoon head is concave and a puncture part for puncturing the transparent film is formed at the front end of the spoon head; and a rubber plug matched with the sample storage test tube is fixed at the tail end of the spoon handle.
The full-automatic nucleic acid extraction instrument for the fecal samples, disclosed by the invention, is characterized in that the spoon handle penetrates through the rubber plug; the moving unit comprises a pneumatic clamping jaw for clamping the end part of the tail end of the spoon handle and a six-axis manipulator for driving the pneumatic clamping jaw to move.
The invention relates to a full-automatic nucleic acid extraction instrument for a stool sample, wherein a visual identification unit comprises a visual camera, a lifting unit driving the visual camera to move longitudinally and a visual light source.
The invention relates to a full-automatic nucleic acid extraction instrument for a fecal sample, wherein a visual sampling assembly further comprises a material moving manipulator, and the material moving manipulator is used for moving a container at a waiting station to a visual detection station and moving the container to a sample processing area after sampling at the visual detection station is completed.
The full-automatic nucleic acid extraction instrument for the fecal sample further comprises an external shell covering the container receiving assembly and the visual sampling assembly, and an air draft unit is arranged on the external shell.
The full-automatic nucleic acid extraction instrument for the fecal samples comprises a container positioning jig, wherein a container positioning groove is formed in the container positioning jig; the sealing unit comprises a transparent film unwinding shaft, a transparent film pulling shaft, a rotating transverse moving unit for driving the transparent film pulling shaft to rotate and transversely move, and a hot-pressing head for pressing the transparent film to the end face of the opening of the container and hot-pressing the transparent film to be adhered to the opening of the container.
The invention relates to a full-automatic nucleic acid extraction instrument for a fecal sample, wherein a conveying belt unit comprises a conveying belt and a conveying belt driving component; the rack sets up the transmission band top, just one side of container constant head tank is opened has the opening that supplies the container to shift out.
The invention has the beneficial effects that: after an open container is placed into a container positioning jig manually or mechanically, a transparent film is sealed on the container by a sealing unit, the container is sent into a waiting station by a conveying belt unit, a code scanning unit scans and identifies the container, a sample in the container is photographed by a visual identification unit through the transparent film, a control host receives and identifies image data of the visual identification unit, the image data is compared with sample data in a sampling database to determine a sampling point, a control moving unit drives a sampling spoon to move to the sampling point for sampling and put into a sample storage test tube corresponding to the last code on the container for storage, and finally the sample storage test tube is sent into a nucleic acid extractor for nucleic acid extraction.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the present invention will be further described with reference to the accompanying drawings and embodiments, wherein the drawings in the following description are only part of the embodiments of the present invention, and for those skilled in the art, other drawings can be obtained without inventive efforts according to the accompanying drawings:
FIG. 1 is a schematic diagram of the overall structure of a fully automatic nucleic acid extraction apparatus for stool samples according to a preferred embodiment of the present invention;
FIG. 2 is a sectional view of a sample spoon of the fully automatic nucleic acid extracting apparatus for stool samples according to the preferred embodiment of the present invention;
FIG. 3 is a schematic view of a positioning fixture of a container of a fecal sample automatic nucleic acid extracting apparatus according to a preferred embodiment of the present invention;
FIG. 4 is a schematic diagram of a rack for placing a stool sample automatic nucleic acid extracting apparatus according to a preferred embodiment of the present invention.
Detailed Description
In order to make the objects, technical solutions and advantages of the embodiments of the present invention clearer, the following will clearly and completely describe the technical solutions in the embodiments of the present invention, and it is obvious that the described embodiments are some embodiments of the present invention, but not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments of the present invention without inventive step, are within the scope of the present invention.
The fully automatic nucleic acid extracting apparatus for stool samples according to the preferred embodiment of the present invention, as shown in fig. 1, with reference to fig. 2-4, includes a container receiving assembly, a visual sampling assembly, and a nucleic acid extracting apparatus 3; the container receiving assembly comprises a container positioning jig 10, a sealing unit 11 for sealing the container by adopting a transparent film, and a conveying belt unit 12 for conveying the sealed container to a waiting station of the visual sampling assembly; the visual sampling assembly comprises a scanning unit 20 for reading a bar code on a container, a visual identification unit 21 for photographing a sample in the container through a transparent film, a sampling spoon 22 extending into the container through the transparent film for sampling, a moving unit 23 for clamping and driving the sampling spoon to move, a sample storage test tube 24 and a control host; the control host computer receives and identifies the image data of the visual identification unit 21, compares the image data with sample data in a sampling database to determine a sampling point, controls the mobile unit 23 to drive the sampling spoon 22 to move to the sampling point for sampling and places the sampling spoon into a sample storage test tube 24 corresponding to the sampling number on the container for storage; the nucleic acid extractor 3 is used for receiving the sample storage test tube and extracting nucleic acid from the internal sample;
after the opened container is placed into the container positioning jig 10 manually or mechanically, the container is sealed by the sealing unit 11 with a transparent film, and then is sent to a waiting station by the conveying belt unit 12, the container is scanned and identified by the code scanning unit 20, then the visual identification unit 21 shoots the sample in the container through the transparent film, the control host receives and identifies the image data of the visual identification unit 21, comparing with sample data in a sampling database to determine a sampling point, controlling the moving unit 23 to drive the sampling spoon 22 to move to the sampling point for sampling and placing the sampling point into the sample storage test tube 24 corresponding to the strip code on the container for storage, finally sending the sample storage test tube 24 into the nucleic acid extractor 3 for nucleic acid extraction, having high automation degree, less manual intervention, high processing efficiency and less sample odor dissipation, meanwhile, the accuracy and the reliability of sampling are greatly improved, and the condition of sample mismatching can not occur;
it should be noted that, when in use, the waiting station can be a code scanning position, or a workpiece can be moved and then code scanned, and the waiting station can be adjusted according to actual conditions; the sample storage test tube can be labeled manually, or special labeling equipment can be arranged, the container label and the label on the sample storage test tube can be associated with the information of the detector for matching, and other existing matching modes can be adopted; the sample storage test tubes and the sample taking spoon can be placed in batches, and can also adopt the transmission assembly to carry out automatic feeding.
Preferably, the sampling spoon 22 comprises a spoon handle 220 and a spoon head 221 which are connected; the spoon head 221 is concave and the front end is formed with a puncture part 2210 for puncturing the transparent film; a rubber plug 2200 matched with a sample storage test tube is fixed at the tail end of the spoon handle 220;
the sampling spoon 22 penetrates through the transparent film by virtue of the puncture part and then continuously extends to a determined sampling point, then the moving unit 23 drives the sampling spoon 22 to rotate to take materials, and the sampling spoon rises to move out of the penetrated part of the transparent film after the materials are taken, so that the materials are taken; in the process, the opening for puncturing the transparent film is small, the smell escapes little, and meanwhile, the sampling spoon can be positioned and prevented from shaking when being inserted, and materials adhered to the outer part of the sampling spoon are scraped when the sampling spoon is taken out, so that the sampling reliability is guaranteed; in addition, through the plug, can be convenient with the sample storage test tube match the lid tightly.
Preferably, the spoon handle 220 extends through the rubber plug 2200; the moving unit 23 comprises a pneumatic clamping jaw 230 for clamping the end part of the tail end of the spoon handle and a six-axis manipulator 231 for driving the pneumatic clamping jaw to move; the clamping and loading operation is convenient.
Preferably, the visual recognition unit 21 includes a visual camera 210, a lifting unit 211 for driving the visual camera to move longitudinally, and a visual light source (not shown); the visual recognition is convenient.
Preferably, the visual sampling assembly further comprises a material moving manipulator 25, wherein the material moving manipulator 25 is used for moving the container at the waiting station to the visual detection station and moving the container to the sample processing area (a processing frame or a processing conveyor belt can be arranged) after the sampling at the visual detection station is completed; the material is conveniently moved and the container after sampling is processed.
Preferably, the full-automatic nucleic acid extraction instrument for the fecal sample further comprises an external shell 4 covering the container receiving assembly and the visual sampling assembly, and an air draft unit 40 is arranged on the external shell 4; convenient and quick air exchange and environment cleanliness maintenance.
Preferably, the container positioning jig 10 comprises a placing frame 100, and a container positioning groove 101 is arranged on the placing frame 100; the sealing unit 11 comprises a transparent film unreeling shaft 110, a transparent film pulling shaft 111, a rotating transverse moving unit for driving the transparent film pulling shaft to rotate and transversely move, and a hot pressing head 112 for pressing the transparent film 5 to the end face of the opening of the container and bonding the transparent film on the opening of the container by hot pressing;
when the device is operated, a container is placed in the container positioning groove 101, then the transverse moving unit drives the transparent film material pulling shaft to transversely move, the transparent film is pulled out from the transparent film unwinding shaft, then the transparent film is bonded on the container in a mode that a hot pressing head is pressed down firstly and then is subjected to hot pressing, the sealing performance is guaranteed, the odor loss is reduced, meanwhile, the waiting time is prolonged, the subsequent visual observation and puncture sampling processes are convenient to perform, and the batch detection is convenient; after hot-pressing sealing is completed, the transverse moving unit is rotated to drive the transparent film material pulling shaft to move and reset and rotate to roll the transparent film so as to wait for the sealing work of the next container;
the rotation traversing unit can be realized by adopting the existing structure, a better mode is that the rotating motor drives the transparent film to pull the material shaft to rotate and receive the material, the traversing lead screw drives the rotating motor to traverse, and other structures can be adopted.
Preferably, the conveyor unit 12 includes a conveyor and a conveyor driving assembly; the placing rack 100 is arranged above the conveying belt, and an opening for moving out the container is formed in one side of the container positioning groove 101; the container is convenient to fix a position and shift out through the rotation direction control of transmission band, and for example the transmission band clockwise rotation can shift out the container constant head tank with the container from the opening, then the transmission band anticlockwise rotation time then can make container laminating container constant head tank inner wall laminate the location.
It will be understood that modifications and variations can be made by persons skilled in the art in light of the above teachings and all such modifications and variations are intended to be included within the scope of the invention as defined in the appended claims.
Claims (8)
1. A full-automatic nucleic acid extraction instrument for a stool sample is characterized by comprising a container receiving assembly, a visual sampling assembly and a nucleic acid extraction instrument; the container receiving assembly comprises a container positioning jig, a sealing unit for sealing the container by adopting a transparent film, and a conveying belt unit for conveying the sealed container to a waiting station of the visual sampling assembly; the visual sampling assembly comprises a scanning unit for reading a bar code on the container, a visual identification unit for photographing a sample in the container through a transparent film, a sampling spoon which penetrates through the transparent film and extends into the container for sampling, a moving unit for clamping and driving the sampling spoon to move, a sample storage test tube and a control host; the control host receives and identifies the image data of the visual identification unit, compares the image data with sample data in a sampling database to determine a sampling point, controls the mobile unit to drive the sampling spoon to move to the sampling point for sampling and places the sampling spoon into a sample storage test tube corresponding to a sampling code on a container for storage; the nucleic acid extractor is used for receiving the sample storage test tube and extracting nucleic acid from the internal sample.
2. The apparatus for automatically extracting nucleic acid from fecal sample according to claim 1, wherein the sampling spoon comprises a handle and a head connected to each other; the spoon head is concave and a puncture part for puncturing the transparent film is formed at the front end of the spoon head; and a rubber plug matched with the sample storage test tube is fixed at the tail end of the spoon handle.
3. The apparatus for automatically extracting nucleic acid from stool sample according to claim 2, wherein the spoon handle penetrates the rubber plug; the moving unit comprises a pneumatic clamping jaw for clamping the end part of the tail end of the spoon handle and a six-axis manipulator for driving the pneumatic clamping jaw to move.
4. The apparatus for automatically extracting nucleic acid from stool sample according to claim 3, wherein the visual recognition unit comprises a visual camera, a lifting unit for moving the visual camera longitudinally, and a visual light source.
5. The apparatus for automatically extracting nucleic acid from a stool sample according to any one of claims 1 to 4, wherein the vision sampling assembly further comprises a material transferring robot for transferring the container at the waiting station to the vision inspection station and transferring the container to the sample processing area after the completion of the sampling at the vision inspection station.
6. The apparatus for automatically extracting nucleic acid from a stool sample according to any one of claims 1 to 4, further comprising an external housing covering the container receiving assembly and the visual sampling assembly, wherein an air draft unit is disposed on the external housing.
7. The apparatus for automatically extracting nucleic acid from fecal samples according to any of claims 1-4, wherein the container positioning fixture comprises a placing frame, the placing frame is provided with a container positioning groove; the sealing unit comprises a transparent film unwinding shaft, a transparent film pulling shaft, a rotating transverse moving unit for driving the transparent film pulling shaft to rotate and transversely move, and a hot-pressing head for pressing the transparent film to the end face of the opening of the container and hot-pressing the transparent film to be adhered to the opening of the container.
8. The apparatus for extracting nucleic acid from a stool sample according to claim 7, wherein the conveyor unit comprises a conveyor and a conveyor driving assembly; the rack sets up the transmission band top, just one side of container constant head tank is opened has the opening that supplies the container to shift out.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110426389.6A CN113125188A (en) | 2021-04-20 | 2021-04-20 | Full-automatic nucleic acid extraction instrument for fecal samples |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110426389.6A CN113125188A (en) | 2021-04-20 | 2021-04-20 | Full-automatic nucleic acid extraction instrument for fecal samples |
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| CN113125188A true CN113125188A (en) | 2021-07-16 |
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| CN202110426389.6A Pending CN113125188A (en) | 2021-04-20 | 2021-04-20 | Full-automatic nucleic acid extraction instrument for fecal samples |
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Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101592653A (en) * | 2008-05-30 | 2009-12-02 | 王海波 | Fully automatic operation instrument for routine inspection of excrement |
| CN102636378A (en) * | 2012-04-10 | 2012-08-15 | 无锡国盛精密模具有限公司 | Machine vision orientation-based biochip microarrayer and positioning method thereof |
| CN102862708A (en) * | 2012-09-24 | 2013-01-09 | 金晖(中山)金属制造有限公司 | Automatic sealing machine for reagents |
| CN206911377U (en) * | 2017-06-21 | 2018-01-23 | 无锡市跨克微营养素有限公司 | Convenient for sampling test tube |
| CN211522155U (en) * | 2019-05-29 | 2020-09-18 | 无锡百泰克生物技术有限公司 | Deodorization device for nucleic acid extractor |
| CN112080421A (en) * | 2020-08-28 | 2020-12-15 | 中国科学院苏州生物医学工程技术研究所 | Ultrahigh-flux full-automatic pathogen nucleic acid detection system and method |
| CN212290683U (en) * | 2020-05-29 | 2021-01-05 | 温州市倍可特医疗器械有限公司 | Test tube sealing machine |
-
2021
- 2021-04-20 CN CN202110426389.6A patent/CN113125188A/en active Pending
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101592653A (en) * | 2008-05-30 | 2009-12-02 | 王海波 | Fully automatic operation instrument for routine inspection of excrement |
| CN102636378A (en) * | 2012-04-10 | 2012-08-15 | 无锡国盛精密模具有限公司 | Machine vision orientation-based biochip microarrayer and positioning method thereof |
| CN102862708A (en) * | 2012-09-24 | 2013-01-09 | 金晖(中山)金属制造有限公司 | Automatic sealing machine for reagents |
| CN206911377U (en) * | 2017-06-21 | 2018-01-23 | 无锡市跨克微营养素有限公司 | Convenient for sampling test tube |
| CN211522155U (en) * | 2019-05-29 | 2020-09-18 | 无锡百泰克生物技术有限公司 | Deodorization device for nucleic acid extractor |
| CN212290683U (en) * | 2020-05-29 | 2021-01-05 | 温州市倍可特医疗器械有限公司 | Test tube sealing machine |
| CN112080421A (en) * | 2020-08-28 | 2020-12-15 | 中国科学院苏州生物医学工程技术研究所 | Ultrahigh-flux full-automatic pathogen nucleic acid detection system and method |
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Application publication date: 20210716 |