CN114910331A - Full-automatic tissue section staining system and staining method - Google Patents
Full-automatic tissue section staining system and staining method Download PDFInfo
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- CN114910331A CN114910331A CN202210615636.1A CN202210615636A CN114910331A CN 114910331 A CN114910331 A CN 114910331A CN 202210615636 A CN202210615636 A CN 202210615636A CN 114910331 A CN114910331 A CN 114910331A
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- 238000010186 staining Methods 0.000 title claims abstract description 26
- 238000007447 staining method Methods 0.000 title abstract description 6
- 239000011521 glass Substances 0.000 claims abstract description 133
- 239000007788 liquid Substances 0.000 claims description 53
- 238000004043 dyeing Methods 0.000 claims description 23
- 239000003153 chemical reaction reagent Substances 0.000 claims description 16
- 238000005485 electric heating Methods 0.000 claims description 12
- 238000007789 sealing Methods 0.000 claims description 11
- 238000000034 method Methods 0.000 claims description 9
- 238000010438 heat treatment Methods 0.000 claims description 7
- 239000006059 cover glass Substances 0.000 claims description 4
- 238000011161 development Methods 0.000 abstract description 10
- 230000001575 pathological effect Effects 0.000 abstract description 2
- 239000000243 solution Substances 0.000 description 15
- 239000008055 phosphate buffer solution Substances 0.000 description 8
- 239000000427 antigen Substances 0.000 description 5
- 102000036639 antigens Human genes 0.000 description 5
- 108091007433 antigens Proteins 0.000 description 5
- 239000012154 double-distilled water Substances 0.000 description 5
- 238000005406 washing Methods 0.000 description 4
- 238000006243 chemical reaction Methods 0.000 description 2
- 238000001035 drying Methods 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 230000036571 hydration Effects 0.000 description 2
- 238000006703 hydration reaction Methods 0.000 description 2
- 238000012544 monitoring process Methods 0.000 description 2
- 238000002791 soaking Methods 0.000 description 2
- 238000010521 absorption reaction Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000012916 chromogenic reagent Substances 0.000 description 1
- 238000012790 confirmation Methods 0.000 description 1
- 238000010276 construction Methods 0.000 description 1
- 238000007599 discharging Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000011049 filling Methods 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 238000011010 flushing procedure Methods 0.000 description 1
- 230000000887 hydrating effect Effects 0.000 description 1
- 230000002055 immunohistochemical effect Effects 0.000 description 1
- 238000011532 immunohistochemical staining Methods 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 238000002372 labelling Methods 0.000 description 1
- 238000011173 large scale experimental method Methods 0.000 description 1
- 238000011068 loading method Methods 0.000 description 1
- 238000010827 pathological analysis Methods 0.000 description 1
- 238000004451 qualitative analysis Methods 0.000 description 1
- 238000004445 quantitative analysis Methods 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 230000007306 turnover Effects 0.000 description 1
Images
Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
- G01N1/31—Apparatus therefor
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
Abstract
The invention discloses a full-automatic tissue section staining system and a staining method, and relates to the technical field of pathological tissue sections. The staining system comprises a glass slide bracket, a glass slide cover plate, a scanning mobile platform and a digital microscope. A plurality of bottom visible slide reading positions are formed on the slide glass bracket, and each slide reading position is used for placing a slide glass; the glass slide cover plate is arranged corresponding to each slide reading position so as to be attached to each glass slide to form a slit space for accommodating the tissue slices. The scanning mobile platform is positioned below the glass slide bracket and can move along the transverse and longitudinal directions, and the digital microscope is carried on the scanning mobile platform. The invention can dynamically monitor the staining degree of each tissue section in the steps of color development and counterstaining, and can stop staining in time when the degree is appropriate, and after the staining is finished, the sections can be directly digitally scanned and stored as digital sections, thus allowing remote staining, reading and retrieving the sections.
Description
Technical Field
The invention relates to the technical field of pathological tissue sections, in particular to a full-automatic tissue section staining system and a staining method.
Background
Immunohistochemical staining is an experimental technique widely used in qualitative and quantitative analysis for observing tissue morphology and performing clinical pathological diagnosis, experimental research and the like. The application of present full-automatic immunohistochemical dyeing instrument is very extensive, but many procedures all are relatively fixed, especially the step such as the coloration after the dyeing is accomplished, counterstain, and the technician can not observe in real time and hold the condition of dyeing, and fixed procedure often leads to the not enough or excessive of coloration or dyeing, is unfavorable for the observation and the judgement of section result. After dyeing is completed, the section is manually sealed and then transferred to a section scanner for scanning, which consumes manpower and time and is not beneficial to large-scale experiment development and rapid turnover. The development of remote medical treatment and the demand of social environment, the demand of the medical field for remote operation increases, and the current dyeing system does not support remote control operation and remote monitoring of dyeing results and can not meet the demand of remote dyeing and film reading.
Disclosure of Invention
The invention aims to provide a full-automatic tissue section dyeing system and a dyeing method, which are used for solving the problems in the background art, are convenient and quick to operate, can dynamically monitor the dyeing degree of each tissue section in the steps of color development and re-dyeing, can stop dyeing in time when the degree is appropriate, and allow a network remote operation machine to remotely dye, read and call the section.
In order to realize the purpose, the invention adopts the following technical scheme:
full-automatic tissue slice staining system includes:
the glass slide rack is provided with a plurality of bottom visible reading positions, and each reading position is used for placing a glass slide;
the glass slide cover plate is arranged corresponding to each slide reading position so as to be attached to each glass slide to form a slit space for accommodating the tissue slices;
the scanning mobile platform is positioned below the slide glass bracket and can move along the transverse direction and the longitudinal direction of the slide glass bracket;
the digital microscope is carried on the scanning mobile platform;
the control module controls the scanning mobile platform to move and controls the digital camera to acquire tissue image information of each film reading position;
and the display module acquires and displays the tissue image information by the control module.
In an alternative embodiment, the slide cover plate is provided with a pure background light panel.
In an alternative embodiment, the edge of the slide cover plate is provided with a sealing ring; the glass slide bracket is provided with a plurality of elastic hinges for hinging the glass slide cover plates and keeping the glass slide cover plates to be jointed with the glass slides.
In an alternative embodiment, each slide cover plate is provided with an electrical heating element and a temperature detector to achieve intracavity heating of the slit space.
In an optional embodiment, the device further comprises a clamping device and a negative pressure suction device; each glass slide cover plate is provided with a liquid inlet port which is a one-way channel which can not be fed out, an electric pipettor is arranged on the clamping device, a plurality of suction needles which can work independently are arranged on the electric pipettor, and reagents are added into the suction needles through the liquid inlet ports; and each glass slide cover plate is provided with a liquid outlet port, the liquid outlet port only can be out but can not be in, the liquid outlet port is connected with the negative pressure suction device through a liquid discharge pipe, and the liquid outlet port is opened and closed through an electric control valve.
In an optional embodiment, the device further comprises a slide storage rack, a horizontal labeler moving block, a labeler laser and a clamping device; a plurality of rows of slide glass placing positions are arranged on the slide glass storage rack, and the horizontal moving block of the labeler can move to the positions corresponding to the slide glass placing positions so as to encode the slide glass; the horizontal moving block of the labeler can move to each slide placing position to push out the slides; the clamping device is provided with a glass slide clamping jaw and a code scanning identifier, and the glass slide clamping jaw identifies and grabs the glass slide with the assistance of the code scanning identifier and the horizontal moving block of the labeler.
In an alternative embodiment, two sides of each column of slide placing positions are respectively provided with a group of indicator lamps, one group of indicator lamps are turned on and off to indicate whether the slide is placed at the current slide placing position, and the other group of indicator lamps are turned on and off to indicate whether the slide is coded.
In an optional embodiment, the clamping device further comprises an electric suction cup, and the electric suction cup is used for sucking the cover glass to complete the cover sheet or sucking the cover plate of the glass slide to complete the uncovering.
In an alternative embodiment, the microscope objective in the digital microscope includes a low power microscope objective for acquiring a dynamic image of each slide and a high power microscope objective for performing slice digital scanning.
Still another object of the present invention is to provide a staining method, which is implemented by using the fully automatic tissue section staining system, wherein a user controls the scanning mobile platform to move on site or remotely, and controls the digital camera to obtain tissue image information of each slide reading position, and the tissue image information is displayed on a display module.
After adopting the technical scheme, compared with the background technology, the invention has the following advantages:
the system is convenient and quick to operate, can dynamically monitor the dyeing degree of each tissue slice in the steps of color development and counterdyeing, can stop dyeing in time when the degree is appropriate, uses the clamping device to assist in liquid changing, slide transferring, slide sealing and other operations, can directly carry out digital scanning on the slices and store the slices as digital slices after dyeing is finished, and allows a machine to be remotely operated through a network to carry out remote dyeing, slice reading and slice taking.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, and it is obvious that the drawings in the following description are only some embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to these drawings without creative efforts.
FIG. 1 is a perspective view of the system configuration of the present invention;
FIG. 2 is a schematic perspective view of a slide mount according to the present invention;
FIG. 3 is a schematic side view of a slide holder according to the present invention;
fig. 4 is a schematic view showing the construction of the gripping apparatus of the present invention;
FIG. 5 is a schematic side view of a slide storage rack of the present invention;
figure 6 is a schematic perspective view of a slide storage rack of the present invention.
Description of reference numerals:
the slide glass comprises a slide glass 1, a tissue 2, a slide glass bracket 3, an elastic hinge 31, a bracket electrical signal interface 32 and a reading position 33;
the glass slide cover plate 4, a liquid inlet port 41, a sealing ring 42, an electric heating glass electrode 43, electric heating glass 44, a liquid outlet port 45, a liquid discharge pipe 451, an electric control valve 46, a valve lead 47, an electric heating glass lead 48 and a temperature detector 49;
a low power microscope 5, a high power microscope objective 6, a digital camera 7, a scanning moving platform 8, a longitudinal guide rail 9, a horizontal guide rail 10 and a glass slide cover plate fulcrum shaft 11;
the gripping device 12, the gripping device data and fluid path interface 121, the gripping device base 122, the gripping device turntable 123, the gripping device first joint 124, the gripping device second joint 125, and the gripping device third joint 126;
the automatic glass slide labeling machine comprises a clamping device expanding head 127, a code scanning recognition device 1271, an electric pipettor 1272, an electric sucker 1273, a glass slide clamping jaw 1274, a glass slide storage rack 13, a glass slide placing position 131, a labeler longitudinal moving table 14, a labeler laser 15, a labeler longitudinal guide rail 16, a labeler horizontal moving block 17, a labeler horizontal guide rail 18 and a glass slide indicator lamp 19;
a control module 20 and a display module 21.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention is described in further detail below with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention.
In the description of the present invention, it is to be understood that the terms "center", "length", "width", "thickness", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", etc. indicate orientations or positional relationships based on those shown in the drawings, and are only for convenience of description and simplicity of description, but do not indicate or imply that the device or element referred to must have a particular orientation, be constructed and operated in a particular orientation, and thus, are not to be construed as limiting the present invention.
In the description of the present invention, it should be noted that, unless otherwise explicitly specified or limited, the terms "mounted," "connected," and "connected" are to be construed broadly, e.g., as meaning either a fixed connection, a removable connection, or an integral connection; can be mechanically or electrically connected; either directly or indirectly through intervening media, either internally or in any other relationship. The specific meanings of the above terms in the present invention can be understood by those skilled in the art according to specific situations.
Examples
Referring to fig. 1-6, the present invention discloses a fully automatic tissue section staining system, which comprises a slide holder 3, a slide cover plate 4, a scanning mobile platform 8, a digital microscope, a control module 20, and a display module 21.
Wherein, a plurality of reading positions with visible bottoms are formed on the glass slide bracket 3, and each reading position is used for placing the glass slide 1. In this embodiment, the slide holder 3 can be provided as a transparent bottom plate so that the reading sites are visible from the bottom.
The scanning moving platform 8 is located below the slide support 3 and can move along the transverse and longitudinal directions of the slide support 3. The digital microscope is mounted on a scanning mobile platform 8. The microscope objective in the digital microscope comprises a low-power microscope objective 5 and a high-power microscope objective 6, wherein the low-power microscope objective 5 is used for acquiring dynamic images of the glass slides 1, and the high-power microscope objective 6 is used for realizing slice digital scanning.
Therefore, the user can control the scanning mobile platform 8 to move on site or remotely through the control module 20, and control the digital camera 7 to acquire the tissue image information of each reading position. The display module 21 acquires and displays the tissue image information by the control module 20.
The slide glass cover plate 4 is arranged corresponding to each slide reading position so as to be attached to each slide glass to form a slit space for accommodating the tissue slice. In this embodiment, the slide cover plate 4 is connected to the slide holder 3 via a slide cover plate support shaft 11, and the slide holder 3 has a flexible hinge 31 to maintain the slide cover plate 4 in close contact with the slide 1. The edges of the slide cover plate 4 are provided with sealing rings 42 to maintain the hermeticity.
The slide glass cover plate 4 is provided with a liquid inlet port 41 and a liquid outlet port 45, in order to facilitate the liquid inlet and outlet, the liquid inlet port 41 is a one-way channel and can only feed in and not feed out, and similarly, the liquid outlet port 45 can only feed out and can not feed in. The liquid outlet 45 is connected with a liquid discharge pipe 451, the liquid discharge pipe 451 is connected with a negative pressure suction device (not shown), and the liquid discharge of the liquid outlet 45 is controlled by an electric control valve 46. Therefore, operations such as reagent filling or liquid changing in the slit space can be realized through opening and closing of the liquid inlet port 41, the liquid outlet port 45 and the electric control valve 46.
In the invention, a pure background light-emitting plate is arranged in the glass slide cover plate 4 to emit light and assist in image observation. In this application, the pure background light-emitting plate is a white light-emitting plate.
In the present invention, the slide glass cover plate 4 is provided with an electrothermal element (electrothermal glass electrode 43, electrothermal glass 44) and a temperature detector 49 to realize the intracavity heating of the slit space and provide heating assistance for the dyeing method.
Specifically, the electric heating glass 44 and the electric heating glass electrode 43 form an electric heating element, and realize intracavity heating in the slit space. The temperature detector 49 is used for detecting the temperature in the cavity to feed back to the control module 20 for temperature control. The electric control valve 46, the electric heating glass electrode 43 and the temperature detector 49 are connected with the bracket electric signal interface 32 on the slide bracket 3 through a valve lead 47 and an electric heating glass lead 48 and controlled by the control module 20.
The invention also comprises a clamping device 12, wherein the clamping device 12 is provided with a clamping device base 122, a liquid feeding driving device (not shown in the figure) is arranged in the clamping device base 122, a negative pressure suction device (not shown in the figure), clamping device data and a liquid path interface 121.
The gripping device base 122 is electrically connected to the control module 20, and the liquid feeding driving device is used for providing mechanical power for the gripping device 12. The gripping device turntable 123 enables the gripping device 12 to rotate 360 ° on the base, and the first joint 124, the second joint 125 and the third joint 126 of the gripping device each have a servo motor (not shown), and can respectively rotate by a large amount to complete various actions.
The gripping device expansion head 127 is provided with a code scanning identifier 1271 which is responsible for image recognition and code scanning confirmation to assist the positioning of the gripping device 12.
Different suction needles are arranged on the electric pipettor 1272 and can work respectively, and the suction needles can extend into the expansion head 127 when being used.
The electric suction cup 1273 is connected to the vacuum suction device of the gripper base 122, and can suck the cover glass to perform the cover-off operation, and can also suck the cover glass of the slide to complete the cover-off operation.
In a preferred embodiment, the present invention further includes a slide storage rack 13, a horizontal movement block 17 for the labeler, and a labeler laser 15. Wherein a plurality of rows of slide placing positions are provided on the slide storage rack 13, and the labeler laser 15 is movable to a position corresponding to each row of slide placing positions to encode the slide 1. The horizontal labeler moving block 17 is movable to each slide placement position to push out the slides.
The slide gripper 1274 recognizes and grips the slide 1 with the aid of the code scanner 1271 and the horizontal movement block 17 of the labeler.
Two sides of each row of slide placing positions 131 are respectively provided with a group of indicator lamps 19, wherein the left group of indicator lamps indicate whether the slide is placed at the current slide placing position or not through on-off, and the other group of indicator lamps (the right group of indicator lamps) indicate whether the slide is coded or not through on-off (the on-off of the indicator lamps is completed through the action of a control module for recording the horizontal moving blocks of the laser and the labeler).
Specifically, the labeler laser 15 is located on the top side and combined with the labeler longitudinal moving stage 14, the labeler laser 15 and the labeler longitudinal moving stage can slide along the labeler longitudinal guide rail 16 to be aligned with different rows of the slide storage rack 13 for cutting, a labeler horizontal guide rail 18 and a labeler horizontal moving block 17 are arranged below the slide storage rack 13, the labeler horizontal moving block 17 moves along the labeler horizontal guide rail 18 to reach different slide placing positions 131 of each row of slides 1 and reach the lower side of a target slide 1, and the labeler horizontal moving block 17 can stretch in the longitudinal direction (the application does not specifically limit the labeler horizontal moving block 17 and can be a micro electric push rod), the slides 1 are pushed upwards, the labeler laser 15 prints coding information on the label area of the slides 1, and after coding is completed, an indicator light on the right side of the slide placing positions 131 is turned on.
Example 2
The invention also discloses a staining method, which is realized by adopting the full-automatic tissue slice staining system, wherein a user controls the scanning mobile platform to move on site or remotely, controls the digital camera 7 to acquire tissue image information of each reading position and displays the tissue image information on the display module 21 so as to dynamically monitor the staining degree of each tissue slice in the steps of color development and counterstaining, and can stop staining timely when the degree is proper.
Specifically, it comprises the following steps:
1. loading and coding a glass slide;
when an experiment is started, a slide glass 1 of a tissue 2 to be stained is placed on a slide glass storage rack 13, a plurality of slide glass placing positions 131 are arranged on the slide glass storage rack 13, indicator lamps 19 are arranged on two sides of the slide glass placing positions 131, the indicator lamp 19 is turned on the left side after the slide glass 1 is placed, the slide glass 1 is shown in the position, after the slide glass 1 is placed, information of each slide glass 1 is input on a control module 20 and comprises an ID number and a staining index, and the control module 20 simultaneously distributes the position of each slide glass 1 on a slide glass support 3.
The horizontal moving block 17 of the labeler moves along the horizontal guide rail 18 of the labeler, reaches the lower part of the target glass slide 1, and pushes the glass slide 1 upwards, the laser 15 of the labeler prints coding information on the label area of the glass slide 1, and after the coding is finished, the indicator lamp 19 on the right side of the glass slide placing position 131 is turned on to indicate that the coding is finished.
2. Transferring the glass slide to an upper seat;
referring to fig. 1, 2, 3 and 4, after the coding is finished, the clamping device 12 takes out each slide 1 in turn with the aid of the code scanning identifier 1271, the horizontal moving block 17 of the labeler pushes out the slide 1 in turn in the vertical direction, the auxiliary clamping device 12 finishes the taking out action, the clamping device 12 places the slide 1 on the slide reading position 33 of the slide support 3, covers the slide cover plate 4, and forms a slit space with the slide 1, and the tissue 2 on the slide 1 is located in the slit space.
3. Baking slices;
the glass slide cover plate 4 generates heat through the electric heating glass 44 to bake the tissue 2 on the glass slide 1, and the temperature is fed back to the control module 20 by the temperature detector 49 to be set.
4. Dewaxing and hydrating;
after the baking, the control module 20 controls the clamping device 12 to suck a sufficient amount of dewaxing reagent through the electric pipettor 1272, the appropriate amount of reagent is respectively added into a slit space between the glass slide cover plate 4 and the glass slide 1 through the liquid inlet port 41 to dewax the tissue 2, the electric heating glass 44 is appropriately heated to assist dewaxing, the reagent is discharged from the liquid outlet port 45 through a liquid discharge pipeline after dewaxing is completed, the liquid discharge operation is controlled by the control module 20 through the electric control valve 46, the hydration reagents are sequentially added until the hydration reagents are completely hydrated finally, the tissue 2 is soaked in a PBS (phosphate buffer solution), and the clamping device 12 is automatically cleaned before the electric pipettor 1272 sucks different reagents.
5. Antigen retrieval;
the control module 20 controls the clamping device 12 to suck sufficient antigen repairing liquid through the electric pipettor 1272, the antigen repairing liquid is added into the glass slide cover plate 4 through the liquid inlet port 41, different antigen repairing liquids can be added according to different antibody requirements, the electrothermal glass 44 is heated to perform antigen repairing, the temperature is recorded by the temperature detector 49, the glass slide cover plate is naturally cooled after the repairing is completed, the reagent is discharged from the liquid outlet port 45, and the PBS solution is added again to perform soaking, washing and discharging.
6. Sealing;
the control module 20 controls the clamping device 12 to suck enough sealing reagent through the electric pipettor 1272, the sealing reagent is added into the slide glass cover plate 4 through the liquid inlet port 41, the control module 20 counts the sealing time, the reagent is discharged from the liquid outlet port 45 after sealing is finished, and the PBS solution is added again for washing and soaking.
7. Changing the liquid and incubating the antibody;
the control module 20 controls the clamping device 12 to sequentially suck different anti-reagents through the electric pipettor 1272, the anti-reagents are added into the glass slide cover plate 4 through the liquid inlet port 41, the PBS solution of each glass slide 1 is fully discharged before the antibodies are added, the control module 20 automatically performs timing after the antibody reagents are added, the clamping device 12 sequentially performs liquid changing, washing, secondary antibody dropping and other operations after the timing is completed, and after the antibody incubation is completed and the washing is performed, the tissue 2 is soaked in the PBS solution.
8. Developing color;
the control module 20 controls the clamping device 12 to be configured and absorb enough chromogenic solution through the electric pipettor 1272, meanwhile, the other absorption needle absorbs enough double distilled water as standby for stopping chromogenic, the chromogenic solution is added into the glass slide cover plate 4 through the liquid inlet port 41, PBS (phosphate buffer solution) of each glass slide 1 is fully discharged before the chromogenic solution is added, and the control module 20 automatically performs timing after the chromogenic reagent is added;
the scanning mobile platform 8 positioned below the glass slide bracket 3 can freely move and focus, when the dyeing process is carried out and a color development solution is added in a color development stage, the scanning mobile platform 8 is controlled by the control module 20 to see a color development real-time image of the tissue 2 shot by the digital camera 7 on the display module 21, the glass slide cover plate 4 can emit light and be used as a white background auxiliary image for observation, the reaction can be stopped and confirmed in time when the color development is proper, the step is usually completed under the low-power microscope 5, and the counterdyeing process is carried out in the same way;
a scanning moving platform 8 is arranged below the slide glass bracket 3, a digital camera 7 is formed by a low-power microscope objective 5, a high-power microscope objective 6 and the digital camera 7 and is arranged on the movable platform 8, a horizontal guide rail 10 and a longitudinal guide rail 9 are arranged on the scanning moving platform 8, the scanning moving platform 8 can move on the horizontal guide rail 10 and slightly move on the longitudinal guide rail 9, when a color developing solution is added, a control module 20 obtains dynamic images of each slide glass 1 by controlling the scanning moving platform 8 and displays the dynamic images on a display module 21 or remotely transmits the dynamic images to a remote user, the control module 20 can automatically identify the color developing degree, when the color developing is proper, the color developing solution is discharged, sufficient double distilled water is added through a clamping device 12 to stop the color developing, the color developing can also be manually stopped by an operator on site or under remote monitoring, after the color developing is finished, the clamping device 12 automatically carries out liquid changing and flushing for a plurality of times, tissue 2 is soaked in double distilled water.
9. Counterdyeing;
the counterstaining procedure was performed in the same manner, and finally the tissue 2 was immersed in the double distilled water vb program.
10. Drying the slices;
after all dyeing reactions are finished, after double distilled water is discharged from the liquid outlet port 45, the electric control valve 46 is continuously opened for a period of time, air is continuously fed from the liquid inlet port 41 to form laminar flow under the drive of the negative pressure suction device, the electric heating glass 44 of the glass slide cover plate 4 assists in heating, the tissue 2 and the glass slide 1 are dried, and after drying is finished, the clamping device 12 opens the glass slide cover plate 4 to prepare mounting;
11. sealing a sheet;
the control module 20 sucks a sufficient amount of mounting solution through the electric pipettor 1272 of the clamping device 12, and drops the mounting solution onto each dry tissue 2 with the aid of the code scanning identifier 1271, and the electric sucker 1273 sucks the mounting solution to perform mounting.
12. Digitally scanning;
after all the operations are finished, the glass slide 1 is positioned on the glass slide bracket 3, the digital camera 7 switches the high power microscope 6, the digital scanning of the glass slide 1 is carried out under the assistance of the mobile platform 8, the image data is automatically named according to the codes and is stored in the control module 20 and displayed on the display module 21, the control module 20 is connected with the internet and can automatically transmit the data to the cloud and a doctor workstation, and after the mounting liquid is fully dried, the clamping device 12 automatically puts the glass slide 1 into the glass slide storage rack 13 again or maintains the glass slide 1 on the glass slide bracket 3.
13. Remote control and film reading;
after the control module 20 is connected with the internet, a remote user can remotely operate the control module 20 to complete the dyeing operation process and check the progress at any time, after the digital scanning of the slide glass 1 is completed, the remote user can check the digital image on the display module 21, after the dyeing is completed, the slide glass 1 is still positioned on the slide glass support 3, the remote user can control the scanning moving platform 8 to read the slide glass, and if the slide glass 1 is placed in the slide glass storage rack 13, the remote user can call the corresponding slide glass 1 through the clamping device 12 and place the slide glass on the slide glass support 3 again for remote reading.
It will be evident to those skilled in the art that the invention is not limited to the details of the foregoing illustrative embodiments, and that the present invention may be embodied in other specific forms without departing from the spirit or essential attributes thereof. The present embodiments are therefore to be considered in all respects as illustrative and not restrictive, the scope of the invention being indicated by the appended claims rather than by the foregoing description, and all changes which come within the meaning and range of equivalency of the claims are therefore intended to be embraced therein. Any reference sign in a claim should not be construed as limiting the claim concerned.
Furthermore, it should be understood that although the present description refers to embodiments, not every embodiment may contain only a single embodiment, and such description is for clarity only, and those skilled in the art should integrate the description, and the embodiments may be combined as appropriate to form other embodiments understood by those skilled in the art.
Claims (10)
1. Full-automatic tissue slice staining system, its characterized in that includes:
the glass slide rack is provided with a plurality of bottom visible reading positions, and each reading position is used for placing a glass slide;
the glass slide cover plate is arranged corresponding to each slide reading position so as to be attached to each glass slide to form a slit space for accommodating the tissue slices;
the scanning moving platform is positioned below the slide glass bracket and can move along the transverse and longitudinal directions of the slide glass bracket;
the digital microscope is carried on the scanning mobile platform;
the control module controls the scanning mobile platform to move and controls the digital camera to acquire tissue image information of each film reading position;
and the display module acquires and displays the tissue image information by the control module.
2. The fully automated tissue slice staining system of claim 1, wherein:
the glass slide cover plate is provided with a pure background light-emitting plate.
3. The fully automated tissue slice staining system of claim 1, wherein:
the edge of the glass slide cover plate is provided with a sealing ring; the glass slide bracket is provided with a plurality of elastic hinges to hinge each glass slide cover plate and keep each glass slide cover plate and the glass slide to be jointed.
4. The fully automated tissue slice staining system of claim 1, wherein:
each glass slide cover plate is provided with an electric heating element and a temperature detector so as to realize the intracavity heating of the slit space.
5. The fully automated tissue slice staining system of claim 1, wherein:
the device also comprises a clamping device and a negative pressure suction device; each glass slide cover plate is provided with a liquid inlet port which is a one-way channel which can not be fed out, an electric pipettor is arranged on the clamping device, a plurality of suction needles which can work independently are arranged on the electric pipettor, and reagents are added into the suction needles through the liquid inlet ports; each glass slide cover plate is provided with a liquid outlet port, the liquid outlet port only can not be led in or out, the liquid outlet port is connected with the negative pressure suction device through a liquid discharge pipe, and the liquid outlet port is opened and closed through an electric control valve.
6. The fully automated tissue slice staining system of claim 1, wherein:
the device also comprises a slide storage rack, a horizontal moving block of the labeler, a laser of the labeler and a clamping device; a plurality of rows of slide glass placing positions are arranged on the slide glass storage rack, and the horizontal moving block of the labeler can move to the positions corresponding to the slide glass placing positions so as to encode the slide glass; the horizontal moving block of the labeler can move to each slide placing position to push out the slide; the clamping device is provided with a glass slide clamping jaw and a code scanning identifier, and the glass slide clamping jaw identifies and grabs the glass slide with the assistance of the code scanning identifier and the horizontal moving block of the labeler.
7. The fully automated tissue slice staining system of claim 6, wherein:
two sides of each row of slide placing positions are respectively provided with a group of indicating lamps, one group of indicating lamps indicate whether the slide is placed at the current slide placing position through on-off, and the other group of indicating lamps indicate whether the slide is coded or not through on-off.
8. The fully automated tissue slice staining system of claim 5 or 6, wherein:
the clamping device further comprises an electric sucker, and the electric sucker is used for adsorbing the cover glass to complete the cover sheet or adsorbing the cover plate of the glass slide to complete the cover opening.
9. The fully automated tissue slice staining system of claim 1, wherein:
the microscope objective in the digital microscope comprises a low-power microscope objective and a high-power microscope objective, wherein the low-power microscope objective is used for acquiring dynamic images of all glass slides, and the high-power microscope objective is used for realizing slice digital scanning.
10. A dyeing method characterized by: the full-automatic tissue section staining system is adopted to realize that a user controls the scanning mobile platform to move on site or remotely, and controls the digital camera to acquire tissue image information of each slide reading position and display the tissue image information on the display module.
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| Application Number | Priority Date | Filing Date | Title |
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| CN202210615636.1A CN114910331A (en) | 2022-05-31 | 2022-05-31 | Full-automatic tissue section staining system and staining method |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116625779A (en) * | 2023-05-23 | 2023-08-22 | 中国人民解放军总医院第四医学中心 | Slide carrier system capable of achieving intelligent self-identification heating |
| CN117782746A (en) * | 2024-02-27 | 2024-03-29 | 江苏成升基因精准医疗科技有限公司 | Slide glass dyeing and sealing integrated machine |
-
2022
- 2022-05-31 CN CN202210615636.1A patent/CN114910331A/en active Pending
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116625779A (en) * | 2023-05-23 | 2023-08-22 | 中国人民解放军总医院第四医学中心 | Slide carrier system capable of achieving intelligent self-identification heating |
| CN116625779B (en) * | 2023-05-23 | 2023-10-31 | 中国人民解放军总医院第四医学中心 | Slide carrier system capable of achieving intelligent self-identification heating |
| CN117782746A (en) * | 2024-02-27 | 2024-03-29 | 江苏成升基因精准医疗科技有限公司 | Slide glass dyeing and sealing integrated machine |
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