CN211374774U - Non-contact immunohistochemical device with linear arrangement - Google Patents

Abstract

The utility model discloses a non-contact immunohistochemical device of linear arranging. This non-contact immunohistochemical device that linear was arranged includes the closing cap, experiment portion and sample transfer portion, both ends are equipped with the opening about the closing cap, closing cap integral seal experiment portion and discharge platform, experiment portion includes the heating module of arranging in proper order from a left side to the right side, a plurality of dyeing jar and wet box module, wet box module top is equipped with the wet lid that can open, sample transfer portion includes the tray, remove the handle, carry slide caliper rule and discharge platform, carry slide caliper rule connect both sides around in the closing cap, the tray is connected on carry slide caliper rule, the removal handle is connected to the tray, discharge platform is located wet box module right side. The utility model has the functions of isolating personnel from volatile harmful gas and protecting the health of experimenters; reduce the risk of damaging the slices, save time and make the operating environment more stable.

Description

Non-contact immunohistochemical device with linear arrangement

Technical Field

The utility model relates to a biomedical instrument, in particular to non-contact immunohistochemical device of linear arranging.

Background

The immunohistochemical method is a technology which combines a labeled specific antibody with a corresponding antigen in a tissue and carries out qualitative positioning determination on the antigen through a certain color development means, and is widely applied to the fields of biological research and clinical medical diagnosis. With the combination of digital microscopic scanning techniques, immunohistochemistry is almost the most popular experimental method for biology and diagnostics. The method mainly comprises the steps of tissue section pretreatment (dewaxing, hydration, antigen retrieval), antibody incubation (primary antibody and secondary antibody incubation), staining (hematoxylin counterstaining, dehydration, transparence), mounting and the like. The materials are transferred too frequently in the operation process, so that the slicing or tissue damage is easily caused, and the experiment environment is polluted. The section transfer process can bring out a volatile reagent, which causes damage to human health.

Although the immunohistochemical experiment method is relatively simple, the conventional operation is long in time and needs hours, and the used reagents such as paraffin, ethanol and particularly xylene are greatly volatilized in the treatment process and can cause health damage to the laboratory personnel who frequently undertake histological diagnosis for a long time, so that the dyeing cylinder is usually placed in a fume cupboard for experiment, the operation is inconvenient, the price of the current commercially available full-automatic immunohistochemical instrument is high, the cost of the matched reagent and the daily maintenance cost are high, the utilization rate of the equipment is low for scientific research institutions and diagnostic institutions with unsaturated experimental requirements, and the resources are idle or wasted.

SUMMERY OF THE UTILITY MODEL

In order to solve one or more of the above problems, the present invention provides a non-contact immunohistochemical device arranged linearly.

According to one aspect of the present invention, the linearly arranged non-contact immunohistochemical device comprises a sealing cap, an experimental part, and a sample transfer part,

openings are arranged at the left end and the right end of the sealing cover, the sealing cover integrally seals the experimental part and the discharging platform,

the experimental part comprises a heating module, a plurality of dyeing tanks and a wet box module which are sequentially arranged from left to right, the top of the wet box module is provided with an openable wet box cover,

the sample transfer part comprises a tray, a moving handle, a carry caliper and a discharging platform, the carry caliper is connected with the front side and the rear side in the sealing cover, the tray is connected with the carry caliper and is connected with the moving handle, the tray can linearly move forwards and backwards and pass through the opening under the action of the horizontal force of the moving handle, the tray can move up and down along the carry caliper under the action of the vertical force of the moving handle,

discharging platform is located wet box module right side, is provided with the buckle with the mutual joint of slide holder on the discharging platform, can place a plurality of section on the slide holder.

In some embodiments, the closing cap comprises a main cap and a movable sub cap, the main cap integrally seals the experimental part, the movable sub cap seals the discharging platform, the front end and the rear end of the main cap and the front end and the rear end of the movable sub cap are provided with openings, and four corners of the lower end of the movable sub cap are respectively provided with a universal wheel.

In some embodiments, the front end of the closing cover is provided with a positioning groove, the middle section of the positioning groove is provided with a plurality of positioning grooves which vertically face downwards, a dyeing cylinder can be arranged in the corresponding area of each positioning groove, and the outer end of the movable handle is positioned in the positioning groove or the positioning groove.

In some embodiments, the carry caliper is slidably connected to the front side and the rear side in the sealing cover, and the carry caliper and the tray can move along the carry groove and the positioning groove.

In some embodiments, two support slide rails are arranged in the closing cover, the carry caliper moves along the support slide rails,

the lower end of the carry caliper is provided with a plurality of rollers, and the rollers roll on the supporting slide rails;

the inner side surface of the carry caliper is connected with a tray through a slide rail slide block.

In some embodiments, the positioning groove, the carry groove and the opening are provided with sealing rubber rings;

in some embodiments, the staining jar is removably attached to the fixed track.

In some embodiments, the cover is made of a transparent material and the lid is made of an opaque material.

In some embodiments, the movable handle is positioned in the closed cover, the movable handle is connected with a servo motor through a belt transmission system, the servo motor drives the movable handle to horizontally move left and right,

the tray is connected with a lifting device, and the lifting device is an air cylinder or an electric cylinder, so that the lifting device drives the tray to move up and down.

The device has the advantages that the reaction device is completely sealed in the sealing cover, the operating personnel and the operation area are isolated, the slice frame is transferred through the sample transfer part, and people only need to externally operate the moving handle without directly contacting slices, so that the device is simple in structure and low in cost; the beneficial effects are as follows: firstly, an operator does not need to be in direct contact with a slice or a slice rack infected with a reagent, and the operator is isolated from volatile harmful gas as much as possible, so that the effects of improving the experimental environment and protecting the health of the experimenter are achieved; secondly, the operation steps which are required to transfer the sample among various devices are integrated into one device. The risk of damaging the slices is reduced, the time is saved, and the operating environment is more stable; reduce the experiment cost, increase the applicable efficiency of reagent consumptive material.

Drawings

Fig. 1 is a schematic front view of a linearly arranged non-contact immunohistochemical device according to an embodiment of the present invention;

FIG. 2 is a schematic top view of the linearly arranged non-contact immunohistochemical device of FIG. 1;

FIG. 3 is a schematic left side view of the linearly arranged non-contact immunohistochemical device of FIG. 2;

FIG. 4 is a schematic view (one) of the sample moving part shown in FIG. 3;

FIG. 5 is a schematic view (II) of the sample-transferring portion shown in FIG. 3;

sample transfer portion 01, experiment portion 02, heating module 021, dyeing jar 022, wet box module 023, wet lid 024, closing cap 1, main lid 10, opening 11, portable branch lid 12, universal wheel 13, advance a groove 14, constant head tank 15, tray 2, removal handle 3, belt drive 31, servo motor 32, elevating gear 33, carry slide caliper 4, gyro wheel 41, slide rail slider 42 supports slide rail 43, ejection of compact platform 5.

Detailed Description

The present invention will be described in further detail with reference to the accompanying drawings. It should be noted that the terms "front," "back," "left," "right," "upper" and "lower" used in the following description refer to directions in the drawings, and the terms "inner" and "outer" refer to directions toward and away from, respectively, the geometric center of a particular component.

Fig. 1 to 5 schematically show XXX according to an embodiment of the present invention. As shown in the figure, the device comprises a closing cover 1, an experimental part 02 and a sample transferring part 01,

openings 11 are arranged at the left end and the right end of the sealing cover 1, the sealing cover 1 integrally seals the experimental part 02 and the discharging platform 5,

the experimental part 02 comprises a heating module 021, a plurality of dyeing cylinders 022 and a wet box module 023 which are sequentially arranged from left to right, wherein the top of the wet box module 023 is provided with an openable wet box cover 024,

the sample transfer part 01 comprises a tray 2, a moving handle 3, a carry caliper 4 and a discharging platform 5, the carry caliper 4 is connected with the front side and the rear side in the sealing cover 1, the tray 2 is connected with the carry caliper 4, the tray 2 is connected with the moving handle 3, the tray 2 can linearly move back and forth under the horizontal force action of the moving handle 3 and pass through the opening 11, the tray 2 can move up and down along the carry caliper 4 under the vertical force action of the moving handle 3,

the discharging platform 5 is positioned at the right side of the wet box module 023, a buckle which is mutually clamped with the slicing frame is arranged on the discharging platform 5, a plurality of slices can be placed on the slicing frame,

tray 2 is outside the opening 11 of left end, place the slicing rack of filling with the section on tray 2, it drives tray 2 and removes to heat in the heating module 021 to remove handle 3, then horizontal migration to a dyeing jar 022, it drives tray 2 downstream entering dyeing jar 022 and reacts to remove handle 3, the reaction is accomplished the back, it resets and horizontal migration to next dyeing jar 022 to remove handle 3 to drive tray 2 rebound, then, it drives tray 2 and gets into wet box module 023 reaction to remove handle 3, finally, it drives tray 2 and gets into ejection of compact platform 5 to remove handle 3, slicing rack and 5 joints of ejection of compact platform become an organic whole, it drives the empty tray 2 and returns the incision 11 of left end to remove handle 3.

The device has the advantages that the reaction device is completely sealed in the sealing cover 1, the operator and the operation area are isolated, the slice frame is transferred through the sample transfer part 01, the operator only needs to externally operate the moving handle 3 without directly contacting slices, the structure is simple, and the cost is low; the beneficial effects are as follows: firstly, an operator does not need to be in direct contact with a slice or a slice rack infected with a reagent, and the operator is isolated from volatile harmful gas as much as possible, so that the effects of improving the experimental environment and protecting the health of the experimenter are achieved; secondly, the operation steps which are required to transfer the sample among various devices are integrated into one device. The risk of damaging the slices is reduced, the time is saved, and the operating environment is more stable; reduce the experiment cost, increase the applicable efficiency of reagent consumptive material.

Preferably, closing cap 1 includes that main lid 10 and portable branch cover 12, and main lid 10 whole seal experiment portion 02, portable branch lid 12 seals ejection of compact platform 5, and main lid 10 and portable branch lid 12 front and back end are equipped with opening 11, and the universal wheel 13 is installed respectively in portable branch lid 12 lower extreme four corners, and after slicing rack and ejection of compact platform 5 joint become an organic whole, portable closing cap 12 and ejection of compact platform 5 can be separated with main lid 10 to remove to the ventilation module. The beneficial effects are as follows: split type closing cap 1 when removing the section of accomplishing the reaction, still keeps operating personnel and the isolated state of section, protects operating personnel, removes moreover and adopts the gyro wheel structure, and it is convenient to remove, greatly reduced experimenter's work burden.

Preferably, the front end of the closing cap 1 is provided with a position inlet groove 14, the middle section of the position inlet groove 14 is provided with a plurality of positioning grooves 15 which are vertically downward, a dyeing cylinder 022 can be arranged in the corresponding area of each positioning groove 15, the outer end of the movable handle 3 is positioned in the position inlet groove 14 or the positioning groove 15, and an operator manually operates to enable the movable handle 3 to horizontally move in the position inlet groove 14 or move up and down in the positioning groove 15. The beneficial effects are as follows: the positioning grooves 14 and 15 are provided, so that the movement of the wafer rack can be further guided, the movement precision is high, and the wafer rack can accurately reach each area.

Preferably, the carry caliper 4 is slidably connected to the front and rear sides in the sealing cover 1, and the carry caliper 4 and the tray 2 can move along the carry groove 14 and the positioning groove 15. The beneficial effects are as follows: the device simple structure, carry slide caliper rule 4 moves along with tray 2 simultaneously.

Preferably, two supporting slide rails 43 are arranged in the closing cover 1, the carry caliper 4 moves along the supporting slide rails 43,

the lower end of the carry caliper 4 is provided with a plurality of rollers 41, and the rollers 41 roll on the support slide rails 43;

the inner side surface of the carry caliper 4 is connected with the tray 2 through a slide rail slide block 42. The automatic slicing machine has the advantages that the slicing frame is conveyed by the rolling mechanism, the operation is stable, no shaking is caused, the friction resistance is small, and the operation is convenient.

Preferably, the positioning groove 15, the position inlet groove 14 and the opening 11 are provided with sealing rubber rings; the beneficial effects are as follows: the sealing rubber ring can further reduce the overflow of harmful gas.

Preferably, the dyeing cylinder 022 is detachably connected to the fixed rail. The beneficial effects are as follows: the combined reaction modules further enlarge the applicability range of the device, and can carry out various experiments related to the tissue slice.

Preferably, the closing cover 1 is made of transparent material, and the wet box cover 024 is made of opaque material. The beneficial effects are as follows: the closing cap 1 made of transparent materials is convenient to observe, and can effectively control the reaction process.

Preferably, the moving handle 3 is positioned in the sealing cover 1, the moving handle 3 is connected with a servo motor 32 through a belt transmission system 31, the servo motor 32 drives the moving handle 3 to move horizontally left and right,

the tray 2 is connected with a lifting device 33, and the lifting device 33 is an air cylinder or an electric cylinder, so that the lifting device 33 drives the tray 2 to move up and down. The beneficial effects are as follows: the electric control structure can further reduce the overflow of harmful gas, seals the whole mobile device and has high safety performance.

One operational step of the device is as follows:

firstly, inserting the tissue slices into a slice rack by the tray 2 outside the opening 11 at the left end, placing the slice rack filled with slices on the tray 2, and closing the transparent sealing cover 3;

secondly, the movable handle 3 drives the tray 2 to move into the heating module 021, a power supply of the heating module 021 is turned on, and after 15 minutes, the tray is started;

thirdly, horizontally moving the tray 2 to a dyeing cylinder 022 added with xylene, driving the tray 2 to move downwards by the movable handle 3 to enter the dyeing cylinder 022 for reaction, driving the tray 2 to move upwards by the movable handle 3 to reset and horizontally move to the next dyeing cylinder 022 after the reaction is finished, and repeatedly circulating the steps by the dyeing cylinders 022;

fourthly, the handle 3 is moved to drive the tray 2 to enter the wet box module 023, the wet box cover 024 is opened, the antibody is dripped on the slice, the wet box cover 024 is closed, and the incubation is carried out for a corresponding time till the antibody is incubated;

fifthly, the movable handle 3 drives the tray 2 to enter the discharging platform 5, the slicing frame and the discharging platform 5 are connected into a whole in a clamping mode, the movable handle 3 drives the unloaded tray 2 to return to the notch 11 at the left end, and the movable type sub-cover 12 and the internal equipment are transferred to the fume hood to be air-dried and sliced.

What has been described above are only some embodiments of the invention. For those skilled in the art, without departing from the inventive concept, several modifications and improvements can be made, which are within the scope of the invention.

Claims (9)

1. A non-contact immunohistochemical device with linear arrangement is characterized by comprising a sealing cover (1), an experimental part (02) and a sample transfer part (01),

openings (11) are arranged at the left end and the right end of the sealing cover (1), the sealing cover (1) integrally seals the experimental part (02) and the discharging platform (5),

the experimental part (02) comprises a heating module (021), a plurality of dyeing cylinders (022) and a wet box module (023) which are sequentially arranged from left to right, wherein the top of the wet box module (023) is provided with an openable wet box cover (024),

the sample transfer part (01) comprises a tray (2), a moving handle (3), a carry caliper (4) and a discharging platform (5), the carry caliper (4) is connected to the front side and the rear side in the sealing cover (1), the tray (2) is connected to the carry caliper (4), the tray (2) is connected to the moving handle (3), the tray (2) can move linearly forwards and backwards under the action of the horizontal force of the moving handle (3) and penetrates through the opening (11), the tray (2) can move up and down along the carry caliper (4) under the action of the vertical force of the moving handle (3),

ejection of compact platform (5) are located wet box module (023) right side, be provided with the buckle with the mutual joint of slide rack on ejection of compact platform (5), can place a plurality of section on the slide rack.

2. The linearly arranged non-contact immunohistochemical device according to claim 1, wherein the closure cap (1) comprises a main cap (10) and a movable sub cap (12), the main cap (10) integrally seals the experimental part (02), the movable sub cap (12) seals the discharging platform (5), the front and rear ends of the main cap (10) and the movable sub cap (12) are provided with the openings (11), and four corners of the lower end of the movable sub cap (12) are respectively provided with universal wheels (13).

3. A linearly arranged non-contact immunohistochemical device according to claim 1, wherein the front end of the closure (1) is provided with a positioning groove (14), the middle section of the positioning groove (14) is provided with a plurality of positioning grooves (15) vertically downwards, each positioning groove (15) is provided with a dyeing cylinder (022) at the corresponding area, and the outer end of the movable handle (3) is located in the positioning groove (14) or the positioning groove (15).

4. A linearly arranged non-contact immunohistochemical device according to claim 3, wherein the carry caliper (4) is slidably connected to the front and back sides of the inside of the closure cap (1), and the carry caliper (4) and the tray (2) can move along the carry groove (14) and the positioning groove (15).

5. A linearly arranged non-contact immunohistochemical device according to claim 4, wherein two support slide rails (43) are arranged in the closure cap (1), the carry caliper (4) moves along the support slide rails (43),

the lower end of the carry caliper (4) is provided with a plurality of rollers (41), and the rollers (41) roll on the supporting slide rails (43);

the inner side surface of the carry caliper (4) is connected with the tray (2) through a sliding rail slider (42).

6. A linearly arranged non-contact immunohistochemical device according to claim 3, wherein the positioning groove (15), the positioning groove (14) and the opening (11) are provided with sealing rubber rings.

7. A linearly arranged non-contact immunohistochemical device according to claim 1, wherein the staining cylinder (022) is detachably connected to a fixed track.

8. A linearly arranged non-contact immunohistochemical device according to claim 1, wherein the closure cap (1) is made of transparent material and the wet box cover (024) is made of opaque material.

9. A linearly arranged non-contact immunohistochemical device according to claim 1, wherein the moving handle (3) is located inside the closure cap (1), the moving handle (3) is connected to a servo motor (32) through a belt transmission system (31), the servo motor (32) drives the moving handle (3) to move horizontally left and right,

the tray (2) is connected with a lifting device (33), and the lifting device (33) is an air cylinder or an electric cylinder, so that the lifting device (33) drives the tray (2) to move up and down.

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