CN113116919A - Traditional Chinese medicine micro-ecological compound preparation for resisting HPV and HSV and preparation method thereof - Google Patents
Traditional Chinese medicine micro-ecological compound preparation for resisting HPV and HSV and preparation method thereof Download PDFInfo
- Publication number
- CN113116919A CN113116919A CN201911354010.4A CN201911354010A CN113116919A CN 113116919 A CN113116919 A CN 113116919A CN 201911354010 A CN201911354010 A CN 201911354010A CN 113116919 A CN113116919 A CN 113116919A
- Authority
- CN
- China
- Prior art keywords
- traditional chinese
- chinese medicine
- cfu
- freeze
- preparation
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Images
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/70—Carbohydrates; Sugars; Derivatives thereof
- A61K31/715—Polysaccharides, i.e. having more than five saccharide radicals attached to each other by glycosidic linkages; Derivatives thereof, e.g. ethers, esters
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/66—Microorganisms or materials therefrom
- A61K35/74—Bacteria
- A61K35/741—Probiotics
- A61K35/744—Lactic acid bacteria, e.g. enterococci, pediococci, lactococci, streptococci or leuconostocs
- A61K35/747—Lactobacilli, e.g. L. acidophilus or L. brevis
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
- A61K47/30—Macromolecular organic or inorganic compounds, e.g. inorganic polyphosphates
- A61K47/36—Polysaccharides; Derivatives thereof, e.g. gums, starch, alginate, dextrin, hyaluronic acid, chitosan, inulin, agar or pectin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
- A61K47/30—Macromolecular organic or inorganic compounds, e.g. inorganic polyphosphates
- A61K47/36—Polysaccharides; Derivatives thereof, e.g. gums, starch, alginate, dextrin, hyaluronic acid, chitosan, inulin, agar or pectin
- A61K47/38—Cellulose; Derivatives thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/0012—Galenical forms characterised by the site of application
- A61K9/0034—Urogenital system, e.g. vagina, uterus, cervix, penis, scrotum, urethra, bladder; Personal lubricants
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/0087—Galenical forms not covered by A61K9/02 - A61K9/7023
- A61K9/0095—Drinks; Beverages; Syrups; Compositions for reconstitution thereof, e.g. powders or tablets to be dispersed in a glass of water; Veterinary drenches
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/02—Suppositories; Bougies; Bases therefor; Ovules
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/06—Ointments; Bases therefor; Other semi-solid forms, e.g. creams, sticks, gels
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/20—Pills, tablets, discs, rods
- A61K9/2004—Excipients; Inactive ingredients
- A61K9/2022—Organic macromolecular compounds
- A61K9/2063—Proteins, e.g. gelatin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L15/00—Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
- A61L15/16—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons
- A61L15/42—Use of materials characterised by their function or physical properties
- A61L15/44—Medicaments
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/12—Antivirals
- A61P31/20—Antivirals for DNA viruses
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/12—Antivirals
- A61P31/20—Antivirals for DNA viruses
- A61P31/22—Antivirals for DNA viruses for herpes viruses
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/20—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices containing or releasing organic materials
- A61L2300/23—Carbohydrates
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/20—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices containing or releasing organic materials
- A61L2300/30—Compounds of undetermined constitution extracted from natural sources, e.g. Aloe Vera
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/40—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices characterised by a specific therapeutic activity or mode of action
- A61L2300/45—Mixtures of two or more drugs, e.g. synergistic mixtures
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Medicinal Chemistry (AREA)
- Pharmacology & Pharmacy (AREA)
- Epidemiology (AREA)
- Virology (AREA)
- Molecular Biology (AREA)
- Engineering & Computer Science (AREA)
- Oncology (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Microbiology (AREA)
- Inorganic Chemistry (AREA)
- Organic Chemistry (AREA)
- Mycology (AREA)
- General Chemical & Material Sciences (AREA)
- Biotechnology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Communicable Diseases (AREA)
- Materials Engineering (AREA)
- Hematology (AREA)
- Urology & Nephrology (AREA)
- Reproductive Health (AREA)
- Gynecology & Obstetrics (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Medicinal Preparation (AREA)
Abstract
A traditional Chinese medicine micro-ecological compound preparation for resisting HPV and HSV is composed of one or more traditional Chinese medicine polysaccharides and one or more probiotic fermented supernatant or concentrate of fermented supernatant, wherein the probiotic refers to one or more of lactobacillus rhamnosus, lactobacillus reuteri, lactobacillus acidophilus and lactobacillus salivarius; the fermentation supernatant refers to liquid or concentrate of probiotic fermentation liquor after thallus is removed. The preparation can be prepared into liquid, solid and semisolid dosage forms for oral administration or external application according to requirements, and is used for resisting HPV and HSV invasion and harm. The method is characterized in that micro-ecological nursing and vaginal normal flora recovery are advocated, the plant polysaccharide formula probiotics can quickly occupy the area and purify the mixed bacteria, the advantages of probiotic colonization are supported, the double insurance of the improvement of body immunity against HPV and the adjustment of micro-ecology against HPV is highlighted, and the double effects of probiotics against HPV and traditional Chinese medicine polysaccharide against HSV are displayed.
Description
Technical Field
The invention belongs to the technical field of medicines, and particularly relates to an anti-HPV and HSV traditional Chinese medicine micro-ecological compound preparation and a preparation method thereof.
Background
The vagina of a healthy female is internally provided with microbial flora mainly comprising dominant bacteria such as lactobacillus, and the healthy microbial flora can maintain the acidic environment of the vagina, enhance the function of local immune barrier, resist the attack of pathogenic bacteria and reduce the risk of vaginal infection of the female. Human Papilloma Virus (HPV) is a papilloma vacuolatum virus A genus of papovaviridae, can cause female cervical cancer, and seriously affects the physical and mental health and reproductive fate of women. There is a correlation between vaginal dysbacteriosis and high risk type human papillomavirus (HR-HPV) infection, and the vaginal flora of HR-HPV infected people is obviously reduced by lactobacillus. In patients with bacterial vaginosis, a decrease in the proportion of lactobacilli and an increase in the proportion of other bacteria can destroy the microenvironment in the vagina, thereby increasing the susceptibility to HPV, indicating that the susceptibility to HPV is associated with a dysbacteriosis of the vagina. In young women, HPV infection is very common and mostly spontaneously remitting, there are sufficient immune defense mechanisms against viruses in HPV infected patients, and only a few women develop persistent infections and may develop Cervical Intraepithelial Neoplasia (CIN). HPV infection can stimulate cellular immunity and humoral immunity, the immune response of the body determines the outcome of human papilloma virus infection, the effective cellular immunity can clear away existing infection, the humoral immunity can prevent the virus of the same subtype from infecting cells again, and immune cells, cytokines and certain virus proteins are related to the immune escape and persistent infection of the virus. Furthermore, HPV-associated vaginal mucosal immune disorders are also associated with HPV infection, including dysfunction and decreased numbers of associated immune cells, and thus, HPV susceptibility and outcome are determined by immunological competence. Herpes Simplex Virus (HSV) is the earliest discovered human herpes virus and is the most common virus in all human viral diseases, including both serotypes HSV-1 and HSV-2. Genital herpes caused by HSV-2 has attracted a great deal of attention, and the emergence of some novel cases suggests that the incidence of herpes is increasing, seriously affecting human public health.
Both HPV and HSV are associated with each other as common genital infectious diseases. According to the report, the infection rates of HSV-2 and HPV of the cervical cancer patients are obviously higher than those of healthy patients, the infection rate of high-risk HPV of the cervical cancer patients is obviously higher than that of the healthy patients, the infection rate of HPV combined with HSV-2 of the cervical cancer patients is obviously higher than that of the healthy patients, and the high-risk HPV infection and the HSV-2 infection are in obvious positive correlation. Thus, there is a close correlation between cervical cancer and HSV-2, HPV genital tract infections. HPV is the most main 1 genital tract infectious virus, HSV-2 is also common and has high clinical importance.
At present, the treatment strategy aiming at HPV and HSV infection takes prevention before disease and prevention after disease as thinking. In the past 20 years, the research and development of vaccines aiming at HPV and HSV have not obvious effect although various scholars make great efforts. The current medicines for clinically treating HSV-2 infection are Acyclovir (ACYCLOvir, ACV) and nucleotide-like derivatives thereof, but because the antiviral spectrum is narrow, the medicines are easy to generate drug resistance and the price is high, so that huge burden is brought to the spirit and the economy of people. The traditional Chinese medicine has the effects of improving the immunity of organisms and regulating flora, can reduce physical therapy and surgical wounds, and has wide prospects. The existing product has single mechanism of anti-HPV and anti-HSV, has limited effect of anti-HPV and anti-HSV, and can not meet the clinical requirement. The patent product is a traditional Chinese medicine microecological compound preparation, has strong HPV and HSV resistance and multiple functions, and mainly inhibits the replication of HPV and HSV by regulating intestinal flora and vaginal flora, enhances the overall and local immunity of an organism and the like to resist HPV and HSV by multiple mechanisms.
Disclosure of Invention
Based on the problems in the background technology, the invention provides a traditional Chinese medicine microecological compound preparation which can regulate intestinal and vaginal flora, enhance immunity and resist HPV and HSV simultaneously and a preparation method thereof.
An anti-HPV and anti-HSV traditional Chinese medicine micro-ecological compound preparation, which consists of one or more traditional Chinese medicine polysaccharides and one or more probiotics fermentation supernatant or concentrate of the fermentation supernatant;
a traditional Chinese medicine micro-ecological compound preparation for resisting HPV and HSV, wherein the traditional Chinese medicine polysaccharide is one or more than one of extracts of rhizoma Polygonati, radix Codonopsis, Poria, flos Lonicerae, Prunellae Spica, radix Isatidis, Ganoderma, Achyranthis radix, and radix astragali;
an anti-HPV and anti-HSV traditional Chinese medicine micro-ecological compound preparation, wherein the probiotics is one or more of lactobacillus rhamnosus, lactobacillus reuteri, lactobacillus acidophilus and lactobacillus salivarius;
the traditional Chinese medicine micro-ecological compound preparation for resisting HPV and HSV is characterized in that fermentation supernatant is obtained by adopting a centrifugal or filtration mode or a low-temperature reduced-pressure concentration, spraying or freeze-drying mode.
The traditional Chinese medicine micro-ecological compound preparation for resisting HPV and HSV is characterized in that the traditional Chinese medicine polysaccharide is prepared by the following steps:
a. weighing each traditional Chinese medicine raw material according to 10-30 parts of rhizoma polygonati, 10-30 parts of codonopsis pilosula, 10-20 parts of poria cocos, 10-30 parts of honeysuckle, 10-30 parts of selfheal, 10-30 parts of isatis root, 10-20 parts of lucid ganoderma, 10-30 parts of achyranthes bidentata and 10-30 parts of astragalus membranaceus, cutting, cleaning, soaking with cold water overnight, adding purified water which is 8-15 times of the weight of the raw materials, carrying out water bath to 90 ℃, keeping the temperature at 90 ℃, decocting for 2 hours, and stirring once every 10 minutes in the decocting process;
b. removing the medicine residues in the step a, collecting medicine liquid, cooling at room temperature, centrifuging for 10 minutes at 10000rpm, removing precipitates, and collecting supernate;
c. c, carrying out alcohol precipitation on the supernatant obtained in the step b, and obtaining crude traditional Chinese medicine polysaccharide after precipitation;
d. washing the crude traditional Chinese medicine polysaccharide in the step c with sterilized purified water, dissolving with 20 times of sterilized purified water at 42 ℃, adding 0.5% of activated carbon according to the proportion after dissolving, adsorbing overnight at 4 ℃, centrifuging for 10 minutes at 10000rpm after adsorption, removing precipitates, and collecting supernatant to be refined traditional Chinese medicine polysaccharide; or refining with chromatography such as Sephadex column to obtain refined Chinese medicinal polysaccharide;
e. d, filtering the supernatant obtained in the step d through a 0.22-micron filter membrane for sterilization, and concentrating by 10 times to obtain a traditional Chinese medicine polysaccharide concentrated solution;
f. and e, carrying out vacuum freeze drying on the Chinese medicinal polysaccharide concentrated solution in the step e to obtain the Chinese medicinal polysaccharide.
A preparation method of a traditional Chinese medicine micro-ecological compound preparation for resisting HPV and HSV comprises the following steps:
culturing probiotic bacteria, taking out fermentation liquor, removing thallus by centrifugation and/or microporous membrane to obtain fermentation supernatant, culturing bacteria to verify no viable bacteria growth, directly making into liquid preparation or vacuum freeze drying to obtain dry powder, and adding Chinese medicinal polysaccharide to make into preparation.
The working mechanism and the beneficial effects of the invention are as follows:
the working mechanism is as follows: the polysaccharide is an important component in the Chinese herbal medicine, and plays an indispensable role in the process of exerting the curative effect of the Chinese herbal medicine. The polysaccharide has multiple activities of regulating immunity, resisting tumor and virus, resisting aging, reducing blood sugar and blood lipid, etc. Many polysaccharides in medicinal plants, such as rhizoma Polygonati polysaccharide, radix Codonopsis polysaccharide, pachyman, flos Lonicerae polysaccharide, Prunellae Spica polysaccharide, radix Isatidis polysaccharide, Ganoderma polysaccharide, Achyranthis radix polysaccharide, and radix astragali polysaccharide, have functions of resisting HSV and enhancing immunity. For example, the polygonatum polysaccharide has remarkable in-vivo and in-vitro anti-HSV effect, the curative effect of 1.5 percent of polygonatum polysaccharide cream on genital scar rash of guinea pigs and people is equivalent to that of ACV, the curative effect of 0.8 percent of polygonatum polysaccharide eye drops on herpes simplex keratitis is superior to that of ACV, and the polygonatum polysaccharide eye drops have anti-inflammatory effect. Meanwhile, the polygonatum polysaccharide cream has wide pharmacological action for treating the genital scar and rash, has the in-vivo antiviral activity equivalent to that of ACV cream, does not have the toxic or side effect of western medicines, and is a potential genital scar and rash resisting creamA medicine for treating herpes. Meanwhile, the polygonatum polysaccharide, the pachyman and the astragalus polysaccharide also have the functions of regulating intestinal microorganisms to form good micro-ecology and promoting the growth of probiotics. For example, the influence of different concentrations of codonopsis pilosula polysaccharide on lactobacillus rhamnosus is studied, and the codonopsis pilosula polysaccharide with 1.5% (m/v) has the greatest influence on the proliferation of lactobacillus rhamnosus, and the maximum viable count of the codonopsis pilosula polysaccharide can reach 2.18 multiplied by 109CFU/ml can lead the fermentation liquor to reach the maximum acid yield 2-3 hours in advance, shorten the fermentation time, better improve the acid resistance, cholate resistance and high temperature resistance of the fermentation liquor, and the utilization rate of the polysaccharide can reach 70.5 percent. The lactobacillus rhamnosus and the lactobacillus reuteri have remarkable effects on maintaining the balance of normal flora in vagina and preventing and treating vaginal infection of women, the 2 strains are all separated from healthy women, and are clinically used as dietary supplements for a long time, no adverse reaction is observed, and the Safety Qualification (QPS) of European Food Safety Authority (EFSA) is obtained. Meanwhile, the polysaccharide is also a good environment carrier and a nutrient source for the field planting and proliferation of the probiotics, and when the polysaccharide coexists with the probiotics, the field planting and proliferation speed of the probiotics is obviously accelerated when the polysaccharides and the probiotics meet the environment with proper temperature, humidity and the like. Therefore, the combination of the two can regulate intestinal flora and vaginal flora, enhance immunity and resist the invasion and harm of HPV and HSV.
The invention has the following beneficial effects:
(1) concern about female health, and protect against HPV and HSV invasion and harm;
(2) advocate micro-ecological nursing, restore normal flora in vagina, remove the pain of dryness;
(3) highlighting the double insurance of improving the immunity of the organism against HPV and correcting the microecology against HPV;
(4) showing double effects of probiotics on resisting HPV and traditional Chinese medicine polysaccharide on resisting HSV;
(5) the plant polysaccharide formula probiotics can promote the growth of probiotics, quickly occupy the area, purify the mixed bacteria and support the advantages of probiotic colonization.
Drawings
The invention is further described with reference to the following figures and examples:
the attached figure 1 illustrates the formula principle of the traditional Chinese medicine micro-ecological compound preparation for resisting HPV and HSV.
Detailed Description
In order that the invention may be more readily understood, reference will now be made to the following examples which are intended to illustrate the invention. It is to be understood that these examples are for illustrative purposes only and are not intended to limit the scope of the present invention, and that specific experimental procedures not mentioned in the following examples are generally conducted in accordance with conventional experimental procedures.
Example 1 preparation of probiotics
(1) Seed preparation for production
Culture of Lactobacillus rhamnosus (Lactobacillus rhamnosus)
Preheating MRS liquid culture medium to 37 ℃, simultaneously inoculating lactobacillus rhamnosus seed liquid according to the inoculation amount of 2%, standing and culturing at 37 ℃ for 18-24 hours until the final viable count reaches 10%8～1010CFU/ml, and harvesting the bacterial liquid to 4 ℃ for storage.
Culture of Lactobacillus reuteri
Preheating TPY liquid culture medium to 37 ℃, simultaneously inoculating lactobacillus reuteri seed liquid according to the inoculation amount of 4%, standing and culturing for 18-24 hours at 37 ℃, and finally counting the viable bacteria until the concentration reaches 108～1010CFU/ml, and harvesting the bacterial liquid to 4 ℃ for storage.
The MRS liquid culture medium is as follows:
10.0g of peptone, 10.0g of beef extract, 5.0g of yeast extract, 2.0g of diammonium hydrogen citrate, 20.0g of glucose, 801.0 ml of tween, 5.0g of sodium acetate, 2.0g of dipotassium hydrogen phosphate, 0.58g of magnesium sulfate, 0.25g of manganese sulfate, 18.0g of agar and 1000ml of distilled water, and the pH value is 6.2-6.4. Sterilizing at 121 deg.C for 15 min.
The TPY liquid culture medium is as follows:
10g of casein, 5g of soybean peptone, 2g of yeast powder, 5g of glucose, 0.5g of L-cysteine, 2g of dipotassium hydrogen phosphate, 0.5g of magnesium chloride, 0.25g of zinc sulfate, 0.15g of calcium chloride, 0.001g of ferric chloride, 801g of tween and 1L of distilled water, the pH value is 6.5, and the sterilization is carried out at 121 ℃ for 20 min.
EXAMPLE 2 preparation of Chinese medicinal polysaccharides
a. Taking 10-30 parts of traditional Chinese medicine polysaccharide, cutting, cleaning, soaking in cold water overnight, adding purified water with the weight 10 times that of the raw materials, carrying out water bath to 90 ℃, keeping the temperature at 90 ℃, decocting for 2 hours, and stirring once every 10 minutes in the decocting process;
b. removing the medicine residues in the step a, collecting medicine liquid, cooling at room temperature, centrifuging for 10 minutes at 10000rpm, removing precipitates, and collecting supernate;
c. c, carrying out alcohol precipitation on the supernatant obtained in the step b, and obtaining crude traditional Chinese medicine polysaccharide after precipitation;
d. washing the crude traditional Chinese medicine polysaccharide in the step c with sterilized purified water, dissolving with 20 times of sterilized purified water at 42 ℃, adding 0.5% of activated carbon according to the proportion after dissolving, adsorbing overnight at 4 ℃, centrifuging for 10 minutes at 10000rpm after adsorption, removing the precipitate, and collecting the supernatant;
e. d, filtering the supernatant obtained in the step d through a 0.22-micron filter membrane for sterilization, and concentrating by 10 times to obtain a traditional Chinese medicine polysaccharide concentrated solution;
f. and e, carrying out vacuum freeze drying on the traditional Chinese medicine polysaccharide concentrated solution in the step e to obtain the polygonatum polysaccharide.
EXAMPLE 3 Freeze-drying protectant preparation
Each 1000ml of phosphate buffer contained the following components: 40-70 g of traditional Chinese medicine polysaccharide, 4-6 g of gelatin and 50-60 g of cane sugar, wherein the traditional Chinese medicine polysaccharide is prepared in the embodiment 2.
The preparation method comprises the following steps: accurately weighing each component, dissolving in 1000ml phosphoric acid buffer solution, filtering with 0.22 μm filter membrane under aseptic condition for sterilization, and storing at 4 deg.C for use.
The phosphate buffer solution is prepared by dissolving 6-10 g of sodium chloride, 0.05-0.5 g of potassium chloride, 1-1.2 g of disodium hydrogen phosphate, 0.05-0.5 g of monopotassium phosphate, 0.05-0.2 g of calcium chloride and 0.05-0.2 g of magnesium chloride containing 6 crystal water in 1000ml of double distilled water, and carrying out autoclaving at 116 ℃ for 30 minutes.
Embodiment 4 a method for preparing an anti-HPV and anti-HSV traditional Chinese medicine micro-ecological compound preparation effervescent tablet:
a. preparing an effervescent material: preparation of the acidic material: weighing 15 parts of citric acid, 10 parts of lactose, 15 parts of EDTA (ethylene diamine tetraacetic acid) and 0.5 part of brilliant blue indicator according to the mass parts, fully mixing the components, drying at 40-65 ℃, and sieving with a sieve of 8-12 meshes to prepare acidic particles for later use; preparation of alkaline material: weighing 15 parts of sodium bicarbonate, 10 parts of lactose and 1 part of brilliant blue indicator according to the mass parts, fully mixing the components, drying at 40-65 ℃, and sieving with a sieve of 8-12 meshes to prepare alkaline particles for later use; and uniformly mixing the alkaline material and the acidic material according to the ratio of 1: 1, adding 0.5-1 part by mass of levamisole, and performing Co-60 radiation sterilization to obtain the effervescent material.
b. The lactobacillus rhamnosus and lactobacillus reuteri bacterial liquid obtained in the example 1 are uniformly mixed and adjusted to 160 hundred million/ml (wherein 80 hundred million/ml of lactobacillus rhamnosus and 80 hundred million/ml of lactobacillus reuteri) to obtain probiotic mixed bacterial liquid.
c. And (c) adding the probiotic mixed bacterial liquid in the step (b) into the freeze-drying protective agent prepared in the embodiment 3 according to the proportion of 1: 1, 1: 2 or 1: 3 (V: V), subpackaging, and carrying out freeze-vacuum drying to prepare the microecological preparation composite freeze-dried powder.
d. Effervescent tablet preparation
And (3) uniformly mixing the freeze-dried preparation prepared by the method with the effervescent material in the step a according to the ratio of 2: 1-3, pressing the mixture into tablets by using a tablet press, and quantitatively subpackaging.
Embodiment 5 a method for preparing anti-HPV and anti-HSV traditional Chinese medicine micro-ecological compound preparation gel preparation:
a. mixing sodium carboxymethylcellulose (27g) and part of glycerol with appropriate amount of purified water, and stirring to make sodium carboxymethylcellulose fully swell;
b. preparing probiotic freeze-dried powder: the lactobacillus rhamnosus bacterial liquid and lactobacillus reuteri bacterial liquid obtained in example 1 were mixed uniformly and adjusted to 160 × 108CFU/ml (wherein Lactobacillus rhamnosus 80X 10)8CFU/ml, Lactobacillus reuteri 80X 108CFU/ml) to obtain the probiotic mixed bacteria liquid. The liquid preparation can be further prepared into powder by vacuum freeze drying. The powder can be used after being dissolved in a solvent (such as water) at the time of use. Optionally adding or not adding the lyoprotectant during lyophilization, and selecting and adding the lyoprotectant, those skilled in the art can select the appropriate lyoprotectant and its amount according to routine operation. For example, in one embodiment,the freeze-drying protective agent is skimmed milk powder, and the addition amount is 6% (w/v);
c. mixing the traditional Chinese medicine polysaccharide (40-70 g) prepared in the embodiment 2 and the probiotic freeze-dried powder (80 multiplied by 10) prepared in the step b8CFU/g (30g), beta-cyclodextrin (80g), sodium citrate (50g) and pullulan (150g), uniformly mixing, and then adding glycerol (120 g of glycerol in total) for dispersion;
d. mixing with above gel matrix, adding purified water to 1000g, stirring, packaging, and packaging.
Embodiment 6 a method for preparing a traditional Chinese medicine micro-ecological compound preparation vaginal suppository for resisting HPV and HSV:
a. preparing probiotic freeze-dried powder: the lactobacillus rhamnosus bacterial liquid and lactobacillus reuteri bacterial liquid obtained in example 1 were mixed uniformly and adjusted to 160 × 108CFU/ml (wherein Lactobacillus rhamnosus 80X 10)8CFU/ml, Lactobacillus reuteri 80X 108CFU/ml) to obtain the probiotic mixed bacteria liquid. The liquid preparation can be further prepared into powder by vacuum freeze drying. The powder can be used after being dissolved in a solvent (such as water) at the time of use. Optionally adding or not adding the lyoprotectant during lyophilization, and selecting and adding the lyoprotectant, those skilled in the art can select the appropriate lyoprotectant and its amount according to routine operation. For example, in one embodiment, the lyoprotectant is skimmed milk powder, added in an amount of 6% (w/v);
b. mixing the traditional Chinese medicine polysaccharide (40-70 g) prepared in the embodiment 2 and the probiotic freeze-dried powder (80 multiplied by 10) prepared in the step a8CFU/g) (30g) are mixed evenly to obtain the mixture of probiotics and traditional Chinese medicine polysaccharide.
c. And (b) taking the mixture of the probiotics and the traditional Chinese medicine polysaccharide in the b as a main raw material, adding microcrystalline cellulose (150g), magnesium stearate (150g), starch (150g) and glucose (150g) as matrixes, and preparing the vaginal suppository.
Example 7 preparation of an anti-HPV and anti-HSV oral solid formulation of a Chinese herbal micro-ecological Compound preparation
a. Preparing probiotic freeze-dried powder: the lactobacillus rhamnosus and lactobacillus reuteri bacterial liquid obtained in example 1 were mixed uniformly and adjusted to 160 × 108CFU (in which Lactobacillus rhamnosus 80X 10)8CFU/ml, Lactobacillus reuteri 80X 108CFU/ml) to obtain the probiotic mixed bacteria liquid. The liquid preparation can be further prepared into powder by vacuum freeze drying. The powder can be used after being dissolved in a solvent (such as water) at the time of use. Optionally adding or not adding the lyoprotectant during lyophilization, and selecting and adding the lyoprotectant, those skilled in the art can select the appropriate lyoprotectant and its amount according to routine operation. For example, in one embodiment, the lyoprotectant is skimmed milk powder, added in an amount of 6% (w/v);
b. mixing the traditional Chinese medicine polysaccharide (40-70 g) prepared in the embodiment 2 and the probiotic freeze-dried powder (80 multiplied by 10) prepared in the step a8CFU/g) (30g) are mixed evenly to obtain a mixture of probiotics and traditional Chinese medicine polysaccharide;
c. preparing and producing, adding proper amount of citric acid, sucrose and dextrin into the mixture of probiotics and traditional Chinese medicine polysaccharide, preparing tablets, pills, granules and capsules, and packaging to obtain the product.
Example 8 preparation of an anti-HPV and anti-HSV Chinese medicinal micro-ecological Compound preparation oral liquid product
a. Preparing probiotic freeze-dried powder: the lactobacillus rhamnosus and lactobacillus reuteri bacterial liquid obtained in example 1 were mixed uniformly and adjusted to 160 × 108CFU (in which Lactobacillus rhamnosus 80X 10)8CFU/ml, Lactobacillus reuteri 80X 108CFU/ml) to obtain the probiotic mixed bacteria liquid. The liquid preparation can be further prepared into powder by vacuum freeze drying. The powder can be used after being dissolved in a solvent (such as water) at the time of use. Optionally adding or not adding the lyoprotectant during lyophilization, and selecting and adding the lyoprotectant, those skilled in the art can select the appropriate lyoprotectant and its amount according to routine operation. For example, in one embodiment, the lyoprotectant is skimmed milk powder, added in an amount of 6% (w/v);
b. mixing the traditional Chinese medicine polysaccharide (40-70 g) prepared in the embodiment 2 and the probiotic freeze-dried powder (80 multiplied by 10) prepared in the step a8CFU/g) (30g) are mixed evenly to obtain a mixture of probiotics and traditional Chinese medicine polysaccharide;
c. and (2) preparing and producing, namely adding a proper amount of citric acid, cane sugar and dextrin into the mixed mixture of the probiotics and the traditional Chinese medicine polysaccharide, adding the citric acid, the cane sugar and the sweetener without sugar into the mixture, adjusting the pH value to 4.5-6.5, uniformly stirring the mixture, filling the mixture, and packaging the mixture to obtain the probiotics-dextrin oral liquid.
Example 9 preparation of a traditional Chinese medicine micro-ecological compound preparation sanitary napkin (panty liner) for resisting HPV and HSV:
a. preparing probiotic freeze-dried powder: the lactobacillus rhamnosus and lactobacillus reuteri bacterial liquid obtained in example 1 were mixed uniformly and adjusted to 160 × 108CFU/ml (wherein Lactobacillus rhamnosus 80X 10)8CFU/ml, Lactobacillus reuteri 80X 108CFU/ml) to obtain the probiotic mixed bacteria liquid. The liquid preparation can be further prepared into powder by vacuum freeze drying. The powder can be used after being dissolved in a solvent (such as water) at the time of use. During freeze-drying, a proper freeze-drying protective agent can be selectively added, and the addition amount is 6% (w/v);
b. mixing the traditional Chinese medicine polysaccharide (40-70 g) prepared in the embodiment 2 and the probiotic freeze-dried powder (80 multiplied by 10) prepared in the step a8CFU/g) (20g) are mixed evenly to obtain a mixture of probiotics and traditional Chinese medicine polysaccharide;
c. and (c) adding a proper amount of the mixture of the probiotics and the traditional Chinese medicine polysaccharide in the step b into the skin contact surface or the interlayer of the sanitary towel (pad) to obtain the sanitary towel.
Claims (17)
1. A traditional Chinese medicine micro-ecological compound preparation for resisting HPV and HSV is composed of one or more traditional Chinese medicine polysaccharides and one or more probiotics fermentation supernatant or concentrate of the fermentation supernatant.
2. The micro-ecological compound preparation of traditional Chinese medicine according to claim 1, wherein the traditional Chinese medicine polysaccharide is one or more extracts of traditional Chinese medicines of rhizoma polygonati, radix codonopsitis, tuckahoe, honeysuckle, selfheal, radix isatidis, ganoderma lucidum, achyranthes and astragalus root.
3. The micro-ecological compound preparation of traditional Chinese medicine according to claim 1, wherein the probiotic bacteria is one or more of lactobacillus rhamnosus, lactobacillus reuteri, lactobacillus acidophilus and lactobacillus salivarius.
4. The microecological compound preparation of Chinese herbs according to claims 1 to 3, wherein the polysaccharides of Chinese herbs are prepared by the following steps:
a. weighing each traditional Chinese medicine raw material according to 10-30 parts of rhizoma polygonati, 10-30 parts of codonopsis pilosula, 10-20 parts of poria cocos, 10-30 parts of honeysuckle, 10-30 parts of selfheal, 10-30 parts of isatis root, 10-20 parts of lucid ganoderma, 10-30 parts of achyranthes bidentata and 10-30 parts of astragalus membranaceus, cutting, cleaning, soaking with cold water overnight, adding purified water which is 8-15 times of the weight of the raw materials, carrying out water bath to 90 ℃, keeping the temperature at 90 ℃, decocting for 2 hours, and stirring once every 10 minutes in the decocting process;
b. removing the medicine residues in the step a, collecting medicine liquid, cooling at room temperature, centrifuging for 10 minutes at 10000rpm, removing precipitates, and collecting supernate;
c. c, carrying out alcohol precipitation on the supernatant obtained in the step b, and obtaining crude traditional Chinese medicine polysaccharide after precipitation;
d. washing the crude traditional Chinese medicine polysaccharide in the step c with sterilized purified water, dissolving with 20 times of sterilized purified water at 42 ℃, adding 0.5% of activated carbon according to the proportion after dissolving, adsorbing overnight at 4 ℃, centrifuging for 10 minutes at 10000rpm after adsorption, removing precipitates, and collecting supernatant to be refined traditional Chinese medicine polysaccharide; or refining with chromatography such as Sephadex column to obtain refined Chinese medicinal polysaccharide;
e. d, filtering the supernatant obtained in the step d through a 0.22-micron filter membrane for sterilization, and concentrating by 10 times to obtain a traditional Chinese medicine polysaccharide concentrated solution;
f. and e, carrying out vacuum freeze drying on the Chinese medicinal polysaccharide concentrated solution in the step e to obtain the Chinese medicinal polysaccharide.
5. The microecological compound preparation according to claim 1, wherein the fermentation supernatant is obtained by centrifugation or filtration, or the fermentation supernatant is further subjected to low-temperature reduced-pressure concentration, spraying or freeze-drying to obtain a concentrate of the fermentation supernatant.
6. The micro-ecological compound preparation of traditional Chinese medicine according to claim 5,it is characterized in that the final viable count in the fermentation supernatant should reach a concentration of 108～1010CFU/ml。
7. The microecological compound preparation of Chinese herbs according to any one of claims 1 to 6, which is in the form of a liquid or a solid or semi-solid.
8. The microecological compound preparation of the Chinese medicinal material according to claim 7, which is in the form of effervescent tablets, oral liquids, fast dissolving tablets, pills, granules, capsules, gels, suppositories, powders, sprays and films.
9. The microecological compound preparation of Chinese medicinal materials according to any one of claims 1 to 6, which is capable of exerting its medicinal effect by being placed in the skin-contacting surface or the interlay of underpants or sanitary napkins (pantiliners).
10. A process for the preparation of a formulation according to claim 1 or 2, comprising the steps of:
culturing probiotic bacteria, taking out fermentation liquor, removing thallus by centrifugation and/or microporous membrane to obtain fermentation supernatant, culturing bacteria to verify no viable bacteria growth, directly making into liquid preparation or vacuum freeze drying to obtain dry powder, and adding Chinese medicinal polysaccharide to make into preparation.
11. The microecological compound preparation of claim 7, wherein the final viable count of the preparation product is 108～1010CFU/ml or 108～1010CFU/g。
12. A process for the preparation of an effervescent tablet as claimed in claim 8, which comprises the steps of:
a. preparing an effervescent material: preparation of the acidic material: weighing 15 parts of citric acid, 10 parts of lactose, 15 parts of EDTA (ethylene diamine tetraacetic acid) and 0.5 part of brilliant blue indicator according to the mass parts, fully mixing the components, drying at 40-65 ℃, and sieving with a sieve of 8-12 meshes to prepare acidic particles for later use; preparation of alkaline material: weighing 15 parts of sodium bicarbonate, 10 parts of lactose and 1 part of brilliant blue indicator according to the mass parts, fully mixing the components, drying at 40-65 ℃, and sieving with a sieve of 8-12 meshes to prepare alkaline particles for later use; uniformly mixing the alkaline material and the acidic material according to the ratio of 1: 1, adding 0.5-1 part by mass of levamisole, and performing Co-60 radiation sterilization to obtain an effervescent material;
b. uniformly mixing lactobacillus rhamnosus and lactobacillus reuteri liquid, and adjusting to 160 hundred million/ml (wherein 80 hundred million/ml of lactobacillus rhamnosus and 80 hundred million/ml of lactobacillus reuteri) to obtain probiotic mixed liquid;
c. adding a freeze-drying protective agent (each 1000ml of phosphate buffer solution contains 40-70 g of traditional Chinese medicine polysaccharide, 4-6 g of gelatin and 50-60 g of cane sugar) into the probiotic mixed bacterial liquid in the step b according to the proportion of 1: 1, 1: 2 or 1: 3 (V: V), subpackaging, and carrying out freeze-vacuum drying to prepare microecological preparation composite freeze-dried powder;
d. effervescent tablet preparation
And (3) uniformly mixing the prepared composite freeze-dried powder and the effervescent material in the step a according to the ratio of 2: 1-3, pressing into tablets by using a tablet press, and quantitatively subpackaging.
13. A process for preparing the gel of claim 8 comprising the steps of:
a. mixing sodium carboxymethylcellulose (27g) and part of glycerol with appropriate amount of purified water, and stirring to make sodium carboxymethylcellulose fully swell;
b. preparing probiotic freeze-dried powder: mixing lactobacillus rhamnosus and lactobacillus reuteri liquid uniformly, and adjusting to 160 × 108CFU (in which Lactobacillus rhamnosus 80X 10)8CFU/ml, Lactobacillus reuteri 80X 108CFU/ml) to obtain probiotic mixed bacteria liquid; during freeze-drying, sucrose is added as a freeze-drying protective agent, and the addition amount is 5% (w/v);
c. mixing traditional Chinese medicine polysaccharide (40-70 g) and probiotic freeze-dried powder (80 multiplied by 10)8CFU/g (30g), beta-cyclodextrin (80g), sodium citrate (50g) and pullulan (150g), uniformly mixing, and then adding glycerol (120 g of glycerol in total) for dispersion;
d. mixing with above gel matrix, adding purified water to 1000g, stirring, packaging, and packaging.
14. A method of preparing a vaginal suppository as claimed in claim 8, which comprises the steps of:
a. preparing probiotic freeze-dried powder: the lactobacillus rhamnosus and lactobacillus reuteri bacterial liquid obtained in example 1 were mixed uniformly and adjusted to 160 × 108CFU (in which Lactobacillus rhamnosus 80X 10)8CFU/ml, Lactobacillus reuteri 80X 108CFU/ml) to obtain the probiotic mixed bacteria liquid. The liquid preparation can be further prepared into powder by vacuum freeze drying. The powder can be used after being dissolved in a solvent (such as water) at the time of use. Optionally adding or not adding the lyoprotectant during lyophilization, and selecting and adding the lyoprotectant, those skilled in the art can select the appropriate lyoprotectant and its amount according to routine operation. For example, in one embodiment, the lyoprotectant is skimmed milk powder, added in an amount of 6% (w/v);
b. mixing the traditional Chinese medicine polysaccharide (40-70 g) prepared in the embodiment 2 and the probiotic freeze-dried powder (80 multiplied by 10) prepared in the step a8CFU/g) (30g) are mixed evenly to obtain the mixture of probiotics and traditional Chinese medicine polysaccharide.
c. And (b) taking the mixture of the probiotics and the traditional Chinese medicine polysaccharide in the b as a main raw material, adding microcrystalline cellulose (150g), magnesium stearate (150g), starch (150g) and glucose (150g) as matrixes, and preparing the vaginal suppository.
15. The method for preparing the oral solid preparation of claim 8, which comprises the steps of:
a. preparing probiotic freeze-dried powder: the lactobacillus rhamnosus and lactobacillus reuteri bacterial liquid obtained in example 1 were mixed uniformly and adjusted to 160 × 108CFU (in which Lactobacillus rhamnosus 80X 10)8CFU/ml, Lactobacillus reuteri 80X 108CFU/ml) to obtain the probiotic mixed bacteria liquid. The liquid preparation can be further prepared into powder by vacuum freeze drying. The powder can be used after being dissolved in a solvent (such as water) at the time of use. Optionally adding or not adding freeze-drying protective agent during freeze-drying, and optionally adding freeze-drying protective agentThe skilled person can select suitable lyoprotectants and amounts thereof according to routine procedures. For example, in one embodiment, the lyoprotectant is skimmed milk powder, added in an amount of 6% (w/v);
b. mixing the traditional Chinese medicine polysaccharide (40-70 g) prepared in the embodiment 2 and the probiotic freeze-dried powder (80 multiplied by 10) prepared in the step a8CFU/g) (30g) are mixed evenly to obtain a mixture of probiotics and traditional Chinese medicine polysaccharide;
c. preparing and producing, adding proper amount of citric acid, sucrose and dextrin into the mixture of probiotics and traditional Chinese medicine polysaccharide, preparing tablets, pills, granules and capsules, and packaging to obtain the product.
16. A method for preparing the oral liquid of claim 8, comprising the steps of:
a. preparing probiotic freeze-dried powder: the lactobacillus rhamnosus and lactobacillus reuteri bacterial liquid obtained in example 1 were mixed uniformly and adjusted to 160 × 108CFU (in which Lactobacillus rhamnosus 80X 10)8CFU/ml, Lactobacillus reuteri 80X 108CFU/ml) to obtain the probiotic mixed bacteria liquid. The liquid preparation can be further prepared into powder by vacuum freeze drying. The powder can be used after being dissolved in a solvent (such as water) at the time of use. Optionally adding or not adding the lyoprotectant during lyophilization, and selecting and adding the lyoprotectant, those skilled in the art can select the appropriate lyoprotectant and its amount according to routine operation. For example, in one embodiment, the lyoprotectant is skimmed milk powder, added in an amount of 6% (w/v);
b. mixing the traditional Chinese medicine polysaccharide (40-70 g) prepared in the embodiment 2 and the probiotic freeze-dried powder (80 multiplied by 10) prepared in the step a8CFU/g) (30g) are mixed evenly to obtain a mixture of probiotics and traditional Chinese medicine polysaccharide;
c. and (2) preparing and producing, namely adding a proper amount of citric acid, cane sugar and dextrin into the mixed mixture of the probiotics and the traditional Chinese medicine polysaccharide, adding the citric acid, the cane sugar and the sweetener without sugar into the mixture, adjusting the pH value to 4.5-6.5, uniformly stirring the mixture, filling the mixture, and packaging the mixture to obtain the probiotics-dextrin oral liquid.
17. A method of making a sanitary napkin (pantiliner) as claimed in claim 8, comprising the steps of:
a. preparing probiotic freeze-dried powder: the lactobacillus rhamnosus and lactobacillus reuteri bacterial liquid obtained in example 1 were mixed uniformly and adjusted to 160 × 108CFU (in which Lactobacillus rhamnosus 80X 10)8CFU/ml, Lactobacillus reuteri 80X 108CFU/ml) to obtain the probiotic mixed bacteria liquid. The liquid preparation can be further prepared into powder by vacuum freeze drying. The powder can be used after being dissolved in a solvent (such as water) at the time of use. During freeze-drying, a proper freeze-drying protective agent can be selectively added, and the addition amount is 6% (w/v);
b. mixing Chinese medicinal polysaccharide (40g) and probiotic lyophilized powder (80 × 10)8CFU/g) (20g) are mixed evenly to obtain a mixture of probiotics and traditional Chinese medicine polysaccharide;
c. and (c) adding a proper amount of the mixture of the probiotics and the traditional Chinese medicine polysaccharide in the step (b) into the skin contact surface or the interlayer of the sanitary towel (pad) to obtain the sanitary towel.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201911354010.4A CN113116919A (en) | 2019-12-26 | 2019-12-26 | Traditional Chinese medicine micro-ecological compound preparation for resisting HPV and HSV and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201911354010.4A CN113116919A (en) | 2019-12-26 | 2019-12-26 | Traditional Chinese medicine micro-ecological compound preparation for resisting HPV and HSV and preparation method thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN113116919A true CN113116919A (en) | 2021-07-16 |
Family
ID=76767197
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201911354010.4A Pending CN113116919A (en) | 2019-12-26 | 2019-12-26 | Traditional Chinese medicine micro-ecological compound preparation for resisting HPV and HSV and preparation method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN113116919A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114698852A (en) * | 2022-04-20 | 2022-07-05 | 成都凝创合炬生物科技集团有限公司 | Multifunctional synbiotic composition with antioxidant effect and preparation |
| CN115895941A (en) * | 2022-09-26 | 2023-04-04 | 吉林农业大学 | Lactobacillus rhamnosus and application thereof |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106692204A (en) * | 2017-03-10 | 2017-05-24 | 泰安大凡神农制药有限公司 | Probiotics composition for alleviating or improving pelvic inflammatory diseases and preparation method thereof |
-
2019
- 2019-12-26 CN CN201911354010.4A patent/CN113116919A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106692204A (en) * | 2017-03-10 | 2017-05-24 | 泰安大凡神农制药有限公司 | Probiotics composition for alleviating or improving pelvic inflammatory diseases and preparation method thereof |
Non-Patent Citations (1)
| Title |
|---|
| 方淑环等: "中药多糖抗病毒作用及其作用机制", 《大理学院学报》 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114698852A (en) * | 2022-04-20 | 2022-07-05 | 成都凝创合炬生物科技集团有限公司 | Multifunctional synbiotic composition with antioxidant effect and preparation |
| CN115895941A (en) * | 2022-09-26 | 2023-04-04 | 吉林农业大学 | Lactobacillus rhamnosus and application thereof |
| CN115895941B (en) * | 2022-09-26 | 2024-04-26 | 吉林农业大学 | Lactobacillus rhamnosus and application thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN106420847B (en) | Composition for assisting in protecting gastric mucosa and/or relieving gastrectasia and microecological preparation thereof | |
| EP1279727B1 (en) | Process for producing preparations of natural medicines | |
| CN108785331B (en) | Composition with qi and blood tonifying function and preparation method and application thereof | |
| CN107319563A (en) | Probiotic composition for strengthening anti-gynaecological imflammation ability | |
| CN1251763A (en) | Process for preparing Chinese medicine 'Qianjin tablets' for treating gynopathy | |
| CN116200306B (en) | Lactobacillus rhamnosus LRa16, and application and product thereof in preparation of medicines for treating genital tract infection | |
| CN113116919A (en) | Traditional Chinese medicine micro-ecological compound preparation for resisting HPV and HSV and preparation method thereof | |
| CN108530551B (en) | Preparation of fritillaria polysaccharide and application of fritillaria polysaccharide in preparation of antitumor drugs | |
| CN112999261A (en) | Natto fermented composition capable of relieving arteriosclerosis and preparation method and application thereof | |
| CN103157095B (en) | Kidney bean phytolectin applications in preparation of human drugs and drug composition thereof | |
| CN111150787A (en) | Traditional Chinese veterinary medicine premix and preparation method thereof | |
| CN117679445A (en) | Composition containing stem cell extracellular vesicles and intestinal probiotics extracellular vesicles and application thereof | |
| CN106317236A (en) | Method for increasing astragalus polysaccharides by using fermentation technology | |
| CN101933950A (en) | Application of Trametes robiniophila Murr aqueous extract | |
| CN108113001B (en) | Probiotic freeze-dried powder for relieving gastric ulcer | |
| CN101450083B (en) | Use of clostridium butyricum in preparing composition for treating newborn feeding intolerance | |
| CN114437997B (en) | Lactobacillus plantarum SF-L38 and application thereof in preparation of blood sugar control product | |
| CN105535743A (en) | Probiotic-fermentation-type lung-dispersing and cough-stopping mixture and preparing method thereof | |
| CN115044518A (en) | Liver-protecting probiotics and application thereof | |
| CN110938563B (en) | Lactobacillus BJ-REBORN001 and application thereof in preparation of helicobacter pylori inhibiting fermentation broth | |
| CN109568430B (en) | Preparation method and application of immunopotentiator | |
| CN102068479B (en) | Chinese medicinal composition for treating chronic urinary tract infection and preparation method thereof | |
| CN101810631A (en) | Application of stachyose in preparing medicine for preventing and curing diarrhea | |
| CN111955719A (en) | Special medical food for treating inflammatory bowel disease and preparation method thereof | |
| CN101607035B (en) | Pharmaceutical composition for treating acute bronchitis in children |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| TA01 | Transfer of patent application right |
Effective date of registration: 20220316 Address after: 116620 Room 302, floor 3, No. 18, digital second road, shuangd port, Dalian Economic and Technological Development Zone, Liaoning Province Applicant after: Dalian wuyinfang Technology Co.,Ltd. Address before: 100095 104-77, building 6, yard 1, gaolizhang Road, Haidian District, Beijing Applicant before: Confucian medicine (Beijing) Technology Co.,Ltd. |
|
| TA01 | Transfer of patent application right | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20210716 |
|
| RJ01 | Rejection of invention patent application after publication |