CN114698852A - Multifunctional synbiotic composition with antioxidant effect and preparation - Google Patents
Multifunctional synbiotic composition with antioxidant effect and preparation Download PDFInfo
- Publication number
- CN114698852A CN114698852A CN202210415205.0A CN202210415205A CN114698852A CN 114698852 A CN114698852 A CN 114698852A CN 202210415205 A CN202210415205 A CN 202210415205A CN 114698852 A CN114698852 A CN 114698852A
- Authority
- CN
- China
- Prior art keywords
- parts
- oligosaccharide
- multifunctional
- polysaccharide
- synbiotic composition
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn
Links
- 235000019722 synbiotics Nutrition 0.000 title claims abstract description 40
- 239000000203 mixture Substances 0.000 title claims abstract description 35
- 230000003078 antioxidant effect Effects 0.000 title claims abstract description 24
- 238000002360 preparation method Methods 0.000 title claims abstract description 10
- 235000013406 prebiotics Nutrition 0.000 claims abstract description 44
- 150000004676 glycans Chemical class 0.000 claims abstract description 42
- 229920001282 polysaccharide Polymers 0.000 claims abstract description 42
- 239000005017 polysaccharide Substances 0.000 claims abstract description 42
- 239000006041 probiotic Substances 0.000 claims abstract description 34
- 235000018291 probiotics Nutrition 0.000 claims abstract description 34
- 241000208340 Araliaceae Species 0.000 claims abstract description 21
- 235000005035 Panax pseudoginseng ssp. pseudoginseng Nutrition 0.000 claims abstract description 21
- 235000003140 Panax quinquefolius Nutrition 0.000 claims abstract description 21
- 235000008434 ginseng Nutrition 0.000 claims abstract description 21
- 241000756042 Polygonatum Species 0.000 claims abstract description 18
- 239000002131 composite material Substances 0.000 claims abstract description 17
- 230000003064 anti-oxidating effect Effects 0.000 claims abstract description 9
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 claims description 36
- 241000220223 Fragaria Species 0.000 claims description 24
- 235000016623 Fragaria vesca Nutrition 0.000 claims description 24
- 235000011363 Fragaria x ananassa Nutrition 0.000 claims description 24
- DLRVVLDZNNYCBX-UHFFFAOYSA-N Polydextrose Polymers OC1C(O)C(O)C(CO)OC1OCC1C(O)C(O)C(O)C(O)O1 DLRVVLDZNNYCBX-UHFFFAOYSA-N 0.000 claims description 24
- HQKMJHAJHXVSDF-UHFFFAOYSA-L magnesium stearate Chemical compound [Mg+2].CCCCCCCCCCCCCCCCCC([O-])=O.CCCCCCCCCCCCCCCCCC([O-])=O HQKMJHAJHXVSDF-UHFFFAOYSA-L 0.000 claims description 24
- 241000901050 Bifidobacterium animalis subsp. lactis Species 0.000 claims description 23
- 241000186604 Lactobacillus reuteri Species 0.000 claims description 23
- 229940009289 bifidobacterium lactis Drugs 0.000 claims description 23
- 229940001882 lactobacillus reuteri Drugs 0.000 claims description 23
- 239000000843 powder Substances 0.000 claims description 15
- 150000001875 compounds Chemical class 0.000 claims description 13
- 229920001202 Inulin Polymers 0.000 claims description 12
- 229920001100 Polydextrose Polymers 0.000 claims description 12
- 239000004376 Sucralose Substances 0.000 claims description 12
- TVXBFESIOXBWNM-UHFFFAOYSA-N Xylitol Natural products OCCC(O)C(O)C(O)CCO TVXBFESIOXBWNM-UHFFFAOYSA-N 0.000 claims description 12
- 235000015165 citric acid Nutrition 0.000 claims description 12
- HEBKCHPVOIAQTA-NGQZWQHPSA-N d-xylitol Chemical compound OC[C@H](O)C(O)[C@H](O)CO HEBKCHPVOIAQTA-NGQZWQHPSA-N 0.000 claims description 12
- FTSSQIKWUOOEGC-RULYVFMPSA-N fructooligosaccharide Chemical compound OC[C@H]1O[C@@](CO)(OC[C@@]2(OC[C@@]3(OC[C@@]4(OC[C@@]5(OC[C@@]6(OC[C@@]7(OC[C@@]8(OC[C@@]9(OC[C@@]%10(OC[C@@]%11(O[C@H]%12O[C@H](CO)[C@@H](O)[C@H](O)[C@H]%12O)O[C@H](CO)[C@@H](O)[C@@H]%11O)O[C@H](CO)[C@@H](O)[C@@H]%10O)O[C@H](CO)[C@@H](O)[C@@H]9O)O[C@H](CO)[C@@H](O)[C@@H]8O)O[C@H](CO)[C@@H](O)[C@@H]7O)O[C@H](CO)[C@@H](O)[C@@H]6O)O[C@H](CO)[C@@H](O)[C@@H]5O)O[C@H](CO)[C@@H](O)[C@@H]4O)O[C@H](CO)[C@@H](O)[C@@H]3O)O[C@H](CO)[C@@H](O)[C@@H]2O)[C@@H](O)[C@@H]1O FTSSQIKWUOOEGC-RULYVFMPSA-N 0.000 claims description 12
- 229940107187 fructooligosaccharide Drugs 0.000 claims description 12
- 235000021255 galacto-oligosaccharides Nutrition 0.000 claims description 12
- 150000003271 galactooligosaccharides Chemical class 0.000 claims description 12
- JYJIGFIDKWBXDU-MNNPPOADSA-N inulin Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)OC[C@]1(OC[C@]2(OC[C@]3(OC[C@]4(OC[C@]5(OC[C@]6(OC[C@]7(OC[C@]8(OC[C@]9(OC[C@]%10(OC[C@]%11(OC[C@]%12(OC[C@]%13(OC[C@]%14(OC[C@]%15(OC[C@]%16(OC[C@]%17(OC[C@]%18(OC[C@]%19(OC[C@]%20(OC[C@]%21(OC[C@]%22(OC[C@]%23(OC[C@]%24(OC[C@]%25(OC[C@]%26(OC[C@]%27(OC[C@]%28(OC[C@]%29(OC[C@]%30(OC[C@]%31(OC[C@]%32(OC[C@]%33(OC[C@]%34(OC[C@]%35(OC[C@]%36(O[C@@H]%37[C@@H]([C@@H](O)[C@H](O)[C@@H](CO)O%37)O)[C@H]([C@H](O)[C@@H](CO)O%36)O)[C@H]([C@H](O)[C@@H](CO)O%35)O)[C@H]([C@H](O)[C@@H](CO)O%34)O)[C@H]([C@H](O)[C@@H](CO)O%33)O)[C@H]([C@H](O)[C@@H](CO)O%32)O)[C@H]([C@H](O)[C@@H](CO)O%31)O)[C@H]([C@H](O)[C@@H](CO)O%30)O)[C@H]([C@H](O)[C@@H](CO)O%29)O)[C@H]([C@H](O)[C@@H](CO)O%28)O)[C@H]([C@H](O)[C@@H](CO)O%27)O)[C@H]([C@H](O)[C@@H](CO)O%26)O)[C@H]([C@H](O)[C@@H](CO)O%25)O)[C@H]([C@H](O)[C@@H](CO)O%24)O)[C@H]([C@H](O)[C@@H](CO)O%23)O)[C@H]([C@H](O)[C@@H](CO)O%22)O)[C@H]([C@H](O)[C@@H](CO)O%21)O)[C@H]([C@H](O)[C@@H](CO)O%20)O)[C@H]([C@H](O)[C@@H](CO)O%19)O)[C@H]([C@H](O)[C@@H](CO)O%18)O)[C@H]([C@H](O)[C@@H](CO)O%17)O)[C@H]([C@H](O)[C@@H](CO)O%16)O)[C@H]([C@H](O)[C@@H](CO)O%15)O)[C@H]([C@H](O)[C@@H](CO)O%14)O)[C@H]([C@H](O)[C@@H](CO)O%13)O)[C@H]([C@H](O)[C@@H](CO)O%12)O)[C@H]([C@H](O)[C@@H](CO)O%11)O)[C@H]([C@H](O)[C@@H](CO)O%10)O)[C@H]([C@H](O)[C@@H](CO)O9)O)[C@H]([C@H](O)[C@@H](CO)O8)O)[C@H]([C@H](O)[C@@H](CO)O7)O)[C@H]([C@H](O)[C@@H](CO)O6)O)[C@H]([C@H](O)[C@@H](CO)O5)O)[C@H]([C@H](O)[C@@H](CO)O4)O)[C@H]([C@H](O)[C@@H](CO)O3)O)[C@H]([C@H](O)[C@@H](CO)O2)O)[C@@H](O)[C@H](O)[C@@H](CO)O1 JYJIGFIDKWBXDU-MNNPPOADSA-N 0.000 claims description 12
- 229940029339 inulin Drugs 0.000 claims description 12
- 239000000832 lactitol Substances 0.000 claims description 12
- 235000010448 lactitol Nutrition 0.000 claims description 12
- VQHSOMBJVWLPSR-JVCRWLNRSA-N lactitol Chemical compound OC[C@H](O)[C@@H](O)[C@@H]([C@H](O)CO)O[C@@H]1O[C@H](CO)[C@H](O)[C@H](O)[C@H]1O VQHSOMBJVWLPSR-JVCRWLNRSA-N 0.000 claims description 12
- 229960003451 lactitol Drugs 0.000 claims description 12
- 235000019359 magnesium stearate Nutrition 0.000 claims description 12
- HEBKCHPVOIAQTA-UHFFFAOYSA-N meso ribitol Natural products OCC(O)C(O)C(O)CO HEBKCHPVOIAQTA-UHFFFAOYSA-N 0.000 claims description 12
- 239000001259 polydextrose Substances 0.000 claims description 12
- 229940035035 polydextrose Drugs 0.000 claims description 12
- 235000013856 polydextrose Nutrition 0.000 claims description 12
- BAQAVOSOZGMPRM-QBMZZYIRSA-N sucralose Chemical compound O[C@@H]1[C@@H](O)[C@@H](Cl)[C@@H](CO)O[C@@H]1O[C@@]1(CCl)[C@@H](O)[C@H](O)[C@@H](CCl)O1 BAQAVOSOZGMPRM-QBMZZYIRSA-N 0.000 claims description 12
- 235000019408 sucralose Nutrition 0.000 claims description 12
- 239000000811 xylitol Substances 0.000 claims description 12
- 235000010447 xylitol Nutrition 0.000 claims description 12
- HEBKCHPVOIAQTA-SCDXWVJYSA-N xylitol Chemical compound OC[C@H](O)[C@@H](O)[C@H](O)CO HEBKCHPVOIAQTA-SCDXWVJYSA-N 0.000 claims description 12
- 229960002675 xylitol Drugs 0.000 claims description 12
- 241000894006 Bacteria Species 0.000 claims description 6
- 235000013361 beverage Nutrition 0.000 claims description 6
- 239000002775 capsule Substances 0.000 claims description 3
- 239000003826 tablet Substances 0.000 claims description 3
- 239000000686 essence Substances 0.000 claims description 2
- 230000000529 probiotic effect Effects 0.000 claims description 2
- 239000002552 dosage form Substances 0.000 claims 1
- 230000000694 effects Effects 0.000 abstract description 15
- 238000009472 formulation Methods 0.000 abstract description 2
- GVJHHUAWPYXKBD-UHFFFAOYSA-N (±)-α-Tocopherol Chemical compound OC1=C(C)C(C)=C2OC(CCCC(C)CCCC(C)CCCC(C)C)(C)CCC2=C1C GVJHHUAWPYXKBD-UHFFFAOYSA-N 0.000 description 14
- 150000003254 radicals Chemical class 0.000 description 12
- 230000000052 comparative effect Effects 0.000 description 8
- 229930003427 Vitamin E Natural products 0.000 description 7
- WIGCFUFOHFEKBI-UHFFFAOYSA-N gamma-tocopherol Natural products CC(C)CCCC(C)CCCC(C)CCCC1CCC2C(C)C(O)C(C)C(C)C2O1 WIGCFUFOHFEKBI-UHFFFAOYSA-N 0.000 description 7
- 239000000243 solution Substances 0.000 description 7
- 235000019165 vitamin E Nutrition 0.000 description 7
- 229940046009 vitamin E Drugs 0.000 description 7
- 239000011709 vitamin E Substances 0.000 description 7
- HHEAADYXPMHMCT-UHFFFAOYSA-N dpph Chemical compound [O-][N+](=O)C1=CC([N+](=O)[O-])=CC([N+]([O-])=O)=C1[N]N(C=1C=CC=CC=1)C1=CC=CC=C1 HHEAADYXPMHMCT-UHFFFAOYSA-N 0.000 description 5
- 239000003963 antioxidant agent Substances 0.000 description 4
- 235000006708 antioxidants Nutrition 0.000 description 4
- 230000006378 damage Effects 0.000 description 4
- 230000007760 free radical scavenging Effects 0.000 description 4
- 238000000034 method Methods 0.000 description 4
- 230000001580 bacterial effect Effects 0.000 description 3
- 238000011156 evaluation Methods 0.000 description 3
- 238000002474 experimental method Methods 0.000 description 3
- 239000001963 growth medium Substances 0.000 description 3
- 230000001737 promoting effect Effects 0.000 description 3
- 239000012488 sample solution Substances 0.000 description 3
- 239000010802 sludge Substances 0.000 description 3
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 description 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 2
- 102000019197 Superoxide Dismutase Human genes 0.000 description 2
- 108010012715 Superoxide dismutase Proteins 0.000 description 2
- 238000002835 absorbance Methods 0.000 description 2
- 239000013641 positive control Substances 0.000 description 2
- WCBPJVKVIMMEQC-UHFFFAOYSA-N 1,1-diphenyl-2-(2,4,6-trinitrophenyl)hydrazine Chemical group [O-][N+](=O)C1=CC([N+](=O)[O-])=CC([N+]([O-])=O)=C1NN(C=1C=CC=CC=1)C1=CC=CC=C1 WCBPJVKVIMMEQC-UHFFFAOYSA-N 0.000 description 1
- 241000186000 Bifidobacterium Species 0.000 description 1
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 1
- 102000016938 Catalase Human genes 0.000 description 1
- 108010053835 Catalase Proteins 0.000 description 1
- ZZZCUOFIHGPKAK-UHFFFAOYSA-N D-erythro-ascorbic acid Natural products OCC1OC(=O)C(O)=C1O ZZZCUOFIHGPKAK-UHFFFAOYSA-N 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 1
- 229940123457 Free radical scavenger Drugs 0.000 description 1
- 108010024636 Glutathione Proteins 0.000 description 1
- 102000006587 Glutathione peroxidase Human genes 0.000 description 1
- 108700016172 Glutathione peroxidases Proteins 0.000 description 1
- 241000186660 Lactobacillus Species 0.000 description 1
- UPYKUZBSLRQECL-UKMVMLAPSA-N Lycopene Natural products CC(=C/C=C/C=C(C)/C=C/C=C(C)/C=C/C1C(=C)CCCC1(C)C)C=CC=C(/C)C=CC2C(=C)CCCC2(C)C UPYKUZBSLRQECL-UKMVMLAPSA-N 0.000 description 1
- BUGBHKTXTAQXES-UHFFFAOYSA-N Selenium Chemical compound [Se] BUGBHKTXTAQXES-UHFFFAOYSA-N 0.000 description 1
- 229930003268 Vitamin C Natural products 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 238000009825 accumulation Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 229910052799 carbon Inorganic materials 0.000 description 1
- 150000001746 carotenes Chemical class 0.000 description 1
- 235000005473 carotenes Nutrition 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 210000002249 digestive system Anatomy 0.000 description 1
- MGJZITXUQXWAKY-UHFFFAOYSA-N diphenyl-(2,4,6-trinitrophenyl)iminoazanium Chemical compound [O-][N+](=O)C1=CC([N+](=O)[O-])=CC([N+]([O-])=O)=C1N=[N+](C=1C=CC=CC=1)C1=CC=CC=C1 MGJZITXUQXWAKY-UHFFFAOYSA-N 0.000 description 1
- 239000012153 distilled water Substances 0.000 description 1
- 231100000673 dose–response relationship Toxicity 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- RWSXRVCMGQZWBV-WDSKDSINSA-N glutathione Chemical compound OC(=O)[C@@H](N)CCC(=O)N[C@@H](CS)C(=O)NCC(O)=O RWSXRVCMGQZWBV-WDSKDSINSA-N 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 238000001727 in vivo Methods 0.000 description 1
- 238000011081 inoculation Methods 0.000 description 1
- 230000000968 intestinal effect Effects 0.000 description 1
- 229940039696 lactobacillus Drugs 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 239000002609 medium Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 235000015816 nutrient absorption Nutrition 0.000 description 1
- 230000003647 oxidation Effects 0.000 description 1
- 238000007254 oxidation reaction Methods 0.000 description 1
- 239000002504 physiological saline solution Substances 0.000 description 1
- 239000002516 radical scavenger Substances 0.000 description 1
- 238000012599 radical scavenging assay Methods 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 229910052711 selenium Inorganic materials 0.000 description 1
- 239000011669 selenium Substances 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
- 239000006228 supernatant Substances 0.000 description 1
- 239000013589 supplement Substances 0.000 description 1
- 230000004083 survival effect Effects 0.000 description 1
- NCYCYZXNIZJOKI-UHFFFAOYSA-N vitamin A aldehyde Natural products O=CC=C(C)C=CC=C(C)C=CC1=C(C)CCCC1(C)C NCYCYZXNIZJOKI-UHFFFAOYSA-N 0.000 description 1
- 235000019154 vitamin C Nutrition 0.000 description 1
- 239000011718 vitamin C Substances 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Images
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/20—Reducing nutritive value; Dietetic products with reduced nutritive value
- A23L33/21—Addition of substantially indigestible substances, e.g. dietary fibres
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/125—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives containing carbohydrate syrups; containing sugars; containing sugar alcohols; containing starch hydrolysates
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/135—Bacteria or derivatives thereof, e.g. probiotics
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
- A23V2400/173—Reuteri
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Mycology (AREA)
- Nutrition Science (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Molecular Biology (AREA)
- Medicinal Preparation (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Abstract
A multifunctional synbiotic composition with antioxidation effect and a preparation thereof relate to the technical field of synbiotic, the synbiotic composition comprises composite prebiotics and probiotics, and the composite prebiotics comprise at least one of polygonatum polysaccharide and ginseng polysaccharide; the formulation includes the synbiotic composition described above. The composite prebiotics and the probiotics form the synbiotic composition, which has growth promotion effects on the probiotics in different degrees, and particularly, the synbiotic composition added with the ginseng polysaccharide and the polygonatum polysaccharide shows a significantly stronger growth promotion effect and an antioxidant effect than the synbiotic composition added with the ginseng polysaccharide or the polygonatum polysaccharide alone.
Description
Technical Field
The invention relates to the technical field of synbiotics, in particular to a multifunctional synbiotics composition with an antioxidant effect and a preparation.
Background
Synbiotics refers to the combined use of probiotics and prebiotics in the hope that the prebiotics can promote the survival and growth of the probiotics. The combination of prebiotics and probiotics makes it possible to obtain the active action of both. The main reason for the existence of synbiotics is that probiotics cannot survive well in the digestive system without being provided with necessary supply by prebiotics, and many reports prove that the synbiotics can increase the number of lactobacillus and bifidobacterium in the body, and partial studies show that people can improve the growth performance, the intestinal tissue morphology and the nutrient absorption by eating the synbiotics.
Numerous studies have confirmed that there are free radicals in the human body, and that excessive free radicals can cause damage to the human body, and that damage to the human body by free radicals is actually an oxidation process, and the human body itself has the ability to scavenge excess free radicals, mainly by an endogenous free radical scavenging system, which includes enzymes such as superoxide dismutase (SOD), catalase, glutathione peroxidase, and antioxidants such as vitamin C, vitamin E, reduced glutathione, carotene, and selenium. However, with the increase of age and the accumulation of exogenous free radicals, free radicals in human bodies are gradually increased, and the capacity of cells for removing the free radicals is gradually reduced, so that various injuries are accumulated. To reduce the harm of free radicals to human body, exogenous free radical scavenger is sought and explored besides the in-vivo free radical scavenging system.
Therefore, it is one of the hot spots of current research to obtain a composition with more effective prebiotic growth promoting effect and antioxidant effect.
Disclosure of Invention
Aiming at the problems of digging the activity of prebiotics on probiotics and combining the prebiotics with the antioxidant effect in the prior art scheme, the invention provides a multifunctional synbiotic composition with the antioxidant effect and a preparation thereof.
The invention provides the following technical scheme: a multifunctional synbiotic composition with antioxidant effect comprises compound prebiotics and probiotics, wherein the compound prebiotics comprise at least one of rhizoma Polygonati polysaccharide and Ginseng radix polysaccharide.
Preferably, the composite prebiotics further comprise polydextrose, inulin, lactitol, strawberry powder, fructo-oligosaccharide, xylo-oligosaccharide, galacto-oligosaccharide, sucralose, citric acid, strawberry essence, magnesium stearate and xylitol.
Preferably, the probiotic comprises lactobacillus reuteri and bifidobacterium lactis.
Preferably, the beverage comprises the following components, by weight, 5 parts of polygonatum polysaccharide, 5 parts of ginseng polysaccharide, 20 parts of polydextrose, 17 parts of inulin, 5 parts of lactitol, 4 parts of strawberry powder, 1 part of fructo-oligosaccharide, 1 part of xylo-oligosaccharide, 1 part of galacto-oligosaccharide, 0.03 part of sucralose, 0.3 part of citric acid, 0.3 part of strawberry essence, 0.05 part of magnesium stearate, 0.5 part of xylitol, 0.04 part of lactobacillus reuteri and 0.04 part of bifidobacterium lactis.
Preferably, the beverage comprises the following components, by weight, 10 parts of polygonatum polysaccharide, 20 parts of polydextrose, 17 parts of inulin, 5 parts of lactitol, 4 parts of strawberry powder, 1 part of fructo-oligosaccharide, 1 part of xylo-oligosaccharide, 1 part of galacto-oligosaccharide, 0.03 part of sucralose, 0.3 part of citric acid, 0.3 part of strawberry essence, 0.05 part of magnesium stearate, 0.5 part of xylitol, 0.04 part of lactobacillus reuteri and 0.04 part of bifidobacterium lactis.
Preferably, the beverage comprises the following components, by weight, 10 parts of ginseng polysaccharide, 20 parts of polydextrose, 17 parts of inulin, 5 parts of lactitol, 4 parts of strawberry powder, 1 part of fructo-oligosaccharide, 1 part of xylo-oligosaccharide, 1 part of galacto-oligosaccharide, 0.03 part of sucralose, 0.3 part of citric acid, 0.3 part of strawberry essence, 0.05 part of magnesium stearate, 0.5 part of xylitol, 0.04 part of lactobacillus reuteri and 0.04 part of bifidobacterium lactis.
A multifunctional synbiotics preparation with antioxidation function is characterized in that: comprises a multifunctional synbiotic composition with antioxidant effect.
Preferably, the formulation is in the form of a powder, tablet or capsule.
The invention has the beneficial effects that: the composite prebiotics and the probiotics form a synbiotic composition, which has growth promotion effects on the probiotics in different degrees, and particularly, the synbiotic composition added with the ginseng polysaccharide and the polygonatum polysaccharide has obviously stronger growth promotion effect and antioxidant effect than the synbiotic composition added with the ginseng polysaccharide or the polygonatum polysaccharide alone.
Drawings
FIG. 1 is a graph showing the result of the DPPH radical scavenging assay of the present invention.
Detailed Description
The embodiments of the present invention will be described in more detail with reference to the accompanying drawings and reference numerals, so that those skilled in the art can implement the embodiments of the present invention after studying the specification. It should be understood that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention.
Example 1
The invention provides a multifunctional synbiotic composition with an anti-oxidation effect, which comprises a compound prebiotic A and probiotics. The compound prebiotics A comprises 5g of polygonatum polysaccharide, 5g of ginseng polysaccharide, 20 g of polydextrose, 17g of inulin, 5g of lactitol, 4g of strawberry powder, 1g of fructo-oligosaccharide, 1g of xylo-oligosaccharide, 1g of galacto-oligosaccharide, 0.03g of sucralose, 0.3g of citric acid, 0.3g of strawberry essence, 0.05g of magnesium stearate and 0.5g of xylitol; the probiotics comprise 0.04 g of lactobacillus reuteri and 0.04 g of bifidobacterium lactis, and the number of bacteria of the lactobacillus reuteri and the bifidobacterium lactis in the probiotics is more than or equal to 10 Log CFU/g.
Example 2
The invention provides a multifunctional synbiotic composition with an anti-oxidation effect, which comprises compound prebiotics B and probiotics. The compound prebiotics B comprises 10 g of ginseng polysaccharide, 20 g of polydextrose, 17g of inulin, 5g of lactitol, 4g of strawberry powder, 1g of fructo-oligosaccharide, 1g of xylo-oligosaccharide, 1g of galacto-oligosaccharide, 0.03g of sucralose, 0.3g of citric acid, 0.3g of strawberry essence, 0.05g of magnesium stearate and 0.5g of xylitol; the probiotics comprise 0.04 g of lactobacillus reuteri and 0.04 g of bifidobacterium lactis, and the number of bacteria of the lactobacillus reuteri and the bifidobacterium lactis in the probiotics is more than or equal to 10 Log CFU/g.
Example 3
The invention provides a multifunctional synbiotic composition with an anti-oxidation effect, which comprises compound prebiotics C and probiotics. The compound prebiotics C comprise 10 g of polygonatum polysaccharide, 20 g of polydextrose, 17g of inulin, 5g of lactitol, 4g of strawberry powder, 1g of fructo-oligosaccharide, 1g of xylo-oligosaccharide, 1g of galacto-oligosaccharide, 0.03g of sucralose, 0.3g of citric acid, 0.3g of strawberry essence, 0.05g of magnesium stearate and 0.5g of xylitol; the probiotics comprise 0.04 g of lactobacillus reuteri and 0.04 g of bifidobacterium lactis, and the number of bacteria of the lactobacillus reuteri and the bifidobacterium lactis in the probiotics is more than or equal to 10 Log CFU/g.
Example 4
The invention provides a multifunctional synbiotic preparation with antioxidation, which comprises a multifunctional synbiotic composition with antioxidation and auxiliary materials acceptable in the prior pharmaceutical technology, and can be prepared into tablets, powder or capsules.
Comparative example 1
A synbiotic composition comprises composite prebiotics D and probiotics, and comprises 30g of polydextrose, 17g of inulin, 5g of lactitol, 4g of strawberry powder, 1g of fructo-oligosaccharide, 1g of xylo-oligosaccharide, 1g of galacto-oligosaccharide, 0.03g of sucralose, 0.3g of citric acid, 0.3g of strawberry essence, 0.05g of magnesium stearate and 0.5g of xylitol; the probiotics comprise 0.04 g of lactobacillus reuteri and 0.04 g of bifidobacterium lactis, and the number of bacteria of the lactobacillus reuteri and the bifidobacterium lactis in the probiotics is more than or equal to 10 Log CFU/g.
Comparative example 2
A mixture of vitamin E and probiotics comprises 0.04 g of Lactobacillus reuteri and 0.04 g of Bifidobacterium lactis, and the number of bacteria of the Lactobacillus reuteri and the Bifidobacterium lactis in the probiotics is more than or equal to 10 Log CFU/g.
The synbiotic composition added with the polygonatum polysaccharide and the ginseng polysaccharide has stronger growth promoting effect on probiotics. The prebiotic activity evaluation experiment for probiotics was performed by dividing the composite prebiotic A, B, C of examples 1, 2 and 3 and the composite prebiotic D of comparative example 1 into A, B, C, D groups.
The prebiotics activity evaluation experimental method comprises the following steps: under the aseptic operation condition, preparing composite prebiotics according to the proportion, adding the composite prebiotics serving as a carbon source into an initial SDM culture medium after fully mixing, and preparing the SDM culture medium with the concentration of 2 g/L; the SDM culture medium is filled in at least two sterile 10 mL EP tubes, each tube is 3 mL, lactobacillus reuteri and bifidobacterium lactis are respectively inoculated according to 3 percent of inoculation amount and cultured in an incubator at 37 ℃, culture solution after culture for 6, 12, 18 and 24 hours is taken out and centrifuged (4000 rpm, 5 min and 4 ℃) to obtain bacterial sludge, physiological saline (0.85 percent NaCl and 3 mL) with the same volume is added, after full mixing, the OD value is measured at the position of 600nm of wavelength by using an ultraviolet-spectrophotometer, and the obtained results are shown in the table I and the table II. The initial SDM medium is purchased from Hongzhou laboratory appliances science and technology Limited of Guangzhou, and the Lactobacillus reuteri (more than or equal to 10 Log CFU/g) and the Bifidobacterium lactis (more than or equal to 10 Log CFU/g) are purchased from Xiandongchi biotechnology Limited.
The OD value is an absorbance value and is in positive correlation with the quantity of probiotics. From the table one and the table two, it can be seen that the complex prebiotics D (group D) was used as the control group, and the complex prebiotics a (group a), the complex prebiotics B (group B), and the complex prebiotics C (group C) added with polygonatum polysaccharide and/or ginseng polysaccharide have growth promoting effects on lactobacillus reuteri and bifidobacterium lactis at different degrees. Compared with the compound prebiotics B, C added with polygonatum polysaccharide or ginseng polysaccharide alone, the prebiotics is prepared by the following steps of 1:1, the composite prebiotics A added with the polygonatum polysaccharide and the ginseng polysaccharide has obviously stronger growth promotion effect and is better than that added separately. In addition, the growth promotion effects of the compound prebiotics A, B, C on different probiotics are different, but obviously, the compound prebiotics A are greatly improved compared with B, C. Therefore, the synbiotic composition containing the composite prebiotics not only supplements the probiotics, but also can promote the growth of the probiotics.
The synbiotic composition added with rhizoma Polygonati polysaccharide and/or Ginseng radix polysaccharide has strong antioxidation effect. In vitro antioxidant activity evaluation experiments were performed for examples 1, 2, 3 and comparative examples 1, 2, respectively.
Specifically, the composite prebiotics A, B, C, D and the vitamin E are weighed according to the mixture ratio of the examples 1, 2 and 3 and the comparative examples 1 and 2, and are sequentially divided into E, F, G, H, I groups, and the five groups are prepared into a composite prebiotics solution and a vitamin E control solution with the concentrations of 1 mg/mL, 2 mg/mL, 3 mg/mL and 4 mg/mL respectively by using distilled water as a solvent; meanwhile, culturing and preparing lactobacillus reuteri and bifidobacterium lactis solutions with the concentration of 10 Log CFU/mL, respectively centrifuging to obtain bacterial sludge, and respectively mixing the bacterial sludge with the 4 groups of composite prebiotics solutions and the 1 group of vitamin E control solutions according to the proportion to obtain 5 groups of sample solutions with different concentrations, wherein the 5 groups of sample solutions correspond to the examples 1, 2 and 3 and the comparative examples 1 and 2 respectively.
1.5 mL of each sample solution is respectively added with 1.5 mL of 0.1 mmol/L DPPH absolute ethanol solution (stored in dark place and prepared for use now), mixed evenly, kept in dark place at room temperature for reaction for 30 min, and then centrifuged (4000 rpm, 5 min, 4 ℃), and an ultraviolet-spectrophotometer is utilized to take supernatant fluid to measure the absorbance value at the wavelength of 517nm, and the DPPH free radical scavenging rate is calculated. The results are shown in FIG. 1.
DPPH is a stable and purple-appearing free radical, the maximum ultraviolet absorption wavelength is 517nm, the method is widely used for evaluating the free radical scavenging activity of an antioxidant, when DPPH reacts with the antioxidant, DPPH is converted into a DPPH-H form without a free radical form, purple color is rapidly faded, and compared with other methods, the method is more convenient and high in sensitivity, and can be used for detecting the antioxidant activity of the antioxidant with lower concentration.
In fig. 1, xxx represents group E (example 1); Δ represents the F group (example 2); ● denotes group G (example 3); o represents group H (comparative example 1); ■ denotes group I (comparative example 2). The results in FIG. 1 show that vitamin E as a positive control shows different degrees of antioxidant activity in other groups, and the free radical clearance rate of E, F, G exceeds 60% when the concentration reaches 4 mg/mL, and the other groups are dose-dependent. Under the premise of the same dosage and concentration, the group E added with the polygonatum polysaccharide and the ginseng polysaccharide (the weight ratio is 1: 1) has higher clearance rate of free radicals, and then the group E is respectively a group G (polygonatum polysaccharide), a group F (ginseng polysaccharide) and a group H (not added with ginseng polysaccharide and polygonatum polysaccharide). When the concentration of the group E is 4 mg/mL, the compound prebiotics A is combined with the lactobacillus reuteri and the bifidobacterium lactis, the clearance rate is higher and is equivalent to the activity of positive control vitamin E, and the similarity is over 95 percent. The clearance rate also gradually increased after the concentration of group E continued to increase.
The foregoing is a detailed description of one or more embodiments of the invention, which is set forth in more detail and is not intended to limit the scope of the invention. It should be noted that, for a person skilled in the art, several variations and modifications can be made without departing from the concept of the invention, and these are all within the scope of protection of the invention. Therefore, the protection scope of the present patent shall be subject to the appended claims.
Claims (8)
1. A multifunctional synbiotic composition with antioxidant effect comprises composite prebiotics and probiotics, and is characterized in that: the compound prebiotics comprise at least one of rhizoma Polygonati polysaccharide and Ginseng radix polysaccharide.
2. The multifunctional synbiotic composition with antioxidant effect as claimed in claim 1, characterized in that: the composite prebiotics also comprise polydextrose, inulin, lactitol, strawberry powder, fructo-oligosaccharide, xylo-oligosaccharide, galacto-oligosaccharide, sucralose, citric acid, strawberry essence, magnesium stearate and xylitol.
3. The multifunctional synbiotic composition with antioxidant effect as claimed in claim 2, characterized in that: the probiotic bacteria comprise lactobacillus reuteri and bifidobacterium lactis.
4. The multifunctional synbiotic composition with antioxidant effect as claimed in claim 3, characterized in that: the beverage comprises the following components, by weight, 5 parts of polygonatum polysaccharide, 5 parts of ginseng polysaccharide, 20 parts of polydextrose, 17 parts of inulin, 5 parts of lactitol, 4 parts of strawberry powder, 1 part of fructo-oligosaccharide, 1 part of xylo-oligosaccharide, 1 part of galacto-oligosaccharide, 0.03 part of sucralose, 0.3 part of citric acid, 0.3 part of strawberry essence, 0.05 part of magnesium stearate, 0.5 part of xylitol, 0.04 part of lactobacillus reuteri and 0.04 part of bifidobacterium lactis.
5. The multifunctional synbiotic composition with antioxidant effect as claimed in claim 3, characterized in that: the beverage comprises the following components, by weight, 10 parts of polygonatum polysaccharide, 20 parts of polydextrose, 17 parts of inulin, 5 parts of lactitol, 4 parts of strawberry powder, 1 part of fructo-oligosaccharide, 1 part of xylo-oligosaccharide, 1 part of galacto-oligosaccharide, 0.03 part of sucralose, 0.3 part of citric acid, 0.3 part of strawberry essence, 0.05 part of magnesium stearate, 0.5 part of xylitol, 0.04 part of lactobacillus reuteri and 0.04 part of bifidobacterium lactis.
6. The multifunctional synbiotic composition with antioxidant effect as claimed in claim 3, characterized in that: the beverage comprises the following components, by weight, 10 parts of ginseng polysaccharide, 20 parts of polydextrose, 17 parts of inulin, 5 parts of lactitol, 4 parts of strawberry powder, 1 part of fructo-oligosaccharide, 1 part of xylo-oligosaccharide, 1 part of galacto-oligosaccharide, 0.03 part of sucralose, 0.3 part of citric acid, 0.3 part of strawberry essence, 0.05 part of magnesium stearate, 0.5 part of xylitol, 0.04 part of lactobacillus reuteri and 0.04 part of bifidobacterium lactis.
7. A multifunctional synbiotics preparation with antioxidation function is characterized in that: comprises a multifunctional synbiotic composition with antioxidant effect.
8. The multifunctional synbiotic preparation with antioxidant effect as claimed in claim 8, characterized in that: the dosage form of the preparation comprises powder, tablets or capsules.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210415205.0A CN114698852A (en) | 2022-04-20 | 2022-04-20 | Multifunctional synbiotic composition with antioxidant effect and preparation |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210415205.0A CN114698852A (en) | 2022-04-20 | 2022-04-20 | Multifunctional synbiotic composition with antioxidant effect and preparation |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN114698852A true CN114698852A (en) | 2022-07-05 |
Family
ID=82175142
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202210415205.0A Withdrawn CN114698852A (en) | 2022-04-20 | 2022-04-20 | Multifunctional synbiotic composition with antioxidant effect and preparation |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN114698852A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116731218A (en) * | 2023-06-09 | 2023-09-12 | 陕西昂煦生物科技有限公司 | Mushroom polysaccharide extract and preparation method and application thereof |
| WO2024013479A1 (en) * | 2022-07-10 | 2024-01-18 | Optibiotix Limted | Compositions and uses thereof |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104127443A (en) * | 2014-07-25 | 2014-11-05 | 吉林省农业科学院 | Lactobacillus-ginseng polysaccharide composition and its preparation method and use |
| CN113116919A (en) * | 2019-12-26 | 2021-07-16 | 儒医(北京)科技有限公司 | Traditional Chinese medicine micro-ecological compound preparation for resisting HPV and HSV and preparation method thereof |
| CN113621535A (en) * | 2021-07-14 | 2021-11-09 | 湖南省中医药研究院 | Composite protective agent for improving survival rate of probiotic freeze-dried powder and preparation method thereof |
-
2022
- 2022-04-20 CN CN202210415205.0A patent/CN114698852A/en not_active Withdrawn
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104127443A (en) * | 2014-07-25 | 2014-11-05 | 吉林省农业科学院 | Lactobacillus-ginseng polysaccharide composition and its preparation method and use |
| CN113116919A (en) * | 2019-12-26 | 2021-07-16 | 儒医(北京)科技有限公司 | Traditional Chinese medicine micro-ecological compound preparation for resisting HPV and HSV and preparation method thereof |
| CN113621535A (en) * | 2021-07-14 | 2021-11-09 | 湖南省中医药研究院 | Composite protective agent for improving survival rate of probiotic freeze-dried powder and preparation method thereof |
Non-Patent Citations (3)
| Title |
|---|
| 孟祥云;汪永锋;杨丽霞;郭树明;王永胜;范强;: "中药多糖抗氧化作用及其机制研究进展", 中华中医药杂志, no. 08 * |
| 苏伟;赵利;刘建涛;陈红兰;龚珍奇;: "黄精多糖抑菌及抗氧化性能研究", 食品科学, no. 08 * |
| 黄镭;柯寒;陈戈辉;郑妍;: "黄精多糖益生元糕的研制及其品质分析", 食品工业, no. 03 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2024013479A1 (en) * | 2022-07-10 | 2024-01-18 | Optibiotix Limted | Compositions and uses thereof |
| CN116731218A (en) * | 2023-06-09 | 2023-09-12 | 陕西昂煦生物科技有限公司 | Mushroom polysaccharide extract and preparation method and application thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| JP2018111711A (en) | Riboflavin, riboflavin phosphate, and physiologically acceptable salt thereof | |
| KR101493302B1 (en) | Processing of natural polysaccharides by selected non-pathogenic microorganisms and methods of making and using the same | |
| CN103582486A (en) | Probiotic bacteria having antioxidant activity and use thereof | |
| EP3808357A1 (en) | Composition and uses thereof | |
| CN114698852A (en) | Multifunctional synbiotic composition with antioxidant effect and preparation | |
| CN103689595A (en) | Soft lactic acid bacteria capsule and preparation method thereof | |
| CN113925923B (en) | Anti-acne and whitening compound probiotic composition and preparation method thereof | |
| CN108498549B (en) | Composition for improving immune function and application thereof | |
| CN115381860B (en) | Composition for protecting alcoholic liver injury and preparation method and application thereof | |
| Li et al. | Synergistic effects of alginate oligosaccharide and cyanidin-3-O-glucoside on the amelioration of intestinal barrier function in mice | |
| JP2003306442A (en) | Preparation having anti-oxidizing ability and immunostimulating ability and method for producing the same | |
| CN116855413B (en) | Bioactive substance for regulating human body microecological balance prepared from lactobacillus rhamnosus YSs069 and application thereof | |
| CN113521114A (en) | Composition for resisting oxidation, protecting oral cavity, regulating immunity and promoting movement and application thereof | |
| CN114984065B (en) | Probiotic composition for improving immunity and preparation method thereof | |
| CN110747140B (en) | Lactobacillus plantarum with selenium adsorption capacity and application thereof | |
| CN117050914B (en) | Acremonium muciniphilum and application thereof in improving GLP-1 secretion | |
| CN118256375B (en) | Lactobacillus reuteri for regulating blood pressure and glycolipid metabolism and application thereof | |
| CN118389372B (en) | Lactobacillus plantarum with functions of losing weight and improving diarrhea | |
| CN116622559B (en) | Lactobacillus casei for producing acetaldehyde dehydrogenase, anti-alcohol probiotic composition and preparation method thereof | |
| CN117143795B (en) | Method for improving oxidation resistance and promoting proliferation of probiotics and composition thereof | |
| CN116396891B (en) | Probiotic composition of lactobacillus acidophilus LA-G80 and bifidobacterium bifidum BB-G90 and application thereof in liver protection | |
| TWI764356B (en) | Anti-aging composition including strains of lactic acid bacteria or a ferment thereof and use of the composition | |
| CN117363524B (en) | Lactobacillus gasseri MY4 and application thereof in preparation of sleep-aiding and whitening medicines | |
| CN117016789A (en) | Selenium-enriched probiotics microecological preparation formula with mercury antagonism function and preparation method and application thereof | |
| CN117384789A (en) | Pediococcus pentosaceus KS5 and application thereof in preparation of antioxidant and anti-aging food and drug |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| WW01 | Invention patent application withdrawn after publication |
Application publication date: 20220705 |
|
| WW01 | Invention patent application withdrawn after publication |