CN105535743A - Probiotic-fermentation-type lung-dispersing and cough-stopping mixture and preparing method thereof - Google Patents
Probiotic-fermentation-type lung-dispersing and cough-stopping mixture and preparing method thereof Download PDFInfo
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Abstract
The invention discloses a probiotic-fermentation-type lung-dispersing and cough-stopping mixture and a preparing method thereof. The probiotic-fermentation-type lung-dispersing and cough-stopping mixture is prepared from a probiotic-fermentation Chinese compound formula. The Chinese compound formula comprises fineleaf schizonepeta herbs, honey-fried radix stemonae, paniculate swallowwort roots, houttuynia cordata, snake gourd fruits, fructus forsythiae, lonicera japonica, fried fructus arctii, thunberg fritillary bulb, radix paeoniae alba, honey-fried mulberry barks, honey-fried tatarian aster roots, honey-fried common coltsfoot flowers, coastal glehnia roots and liquorice. The preparing method includes the steps that the materials in the Chinese compound formula are decocted with water and then concentrated, glucose, yeast powder, yeast extract and peptone are added or not added, inorganic salt is added, cane sugar and sucralose are added or not added, and the mixture is prepared through probiotic fermentation. Clinical experiments prove that the probiotic-fermentation-type lung-dispersing and cough-stopping mixture has the remarkable curative effect on coughing, and is free of toxic and side effects, good in taste and suitable for people of all ages, and patients can easily accept the probiotic-fermentation-type lung-dispersing and cough-stopping mixture.
Description
Technical field
The present invention relates to a kind of probiotics fermention Chinese medicine and preparation method thereof with wind and heat dispersing removing toxic substances, dispersing lung-QI and dissipating phlegm cough-relieving effect, belong to medical art.
Background technology
Cough refer to that abnormal rising of lung-QI is uttered a word, or double see cough the liquid person that spits.It is a common disease of lung system illness, the Different types of etiopathogenises of diseases caused by exogenous pathogenic factor or internal injury, all can cause impairment of dispersing and descending function of the lung, abnormal rising of lung-QI and coughing.Cough is the syndrome with independence, is again a symptom of lung system various diseases.Take cough as the disease of cardinal symptom, the diseases such as visible respiratory tract infection, acute/chronic bronchitis, bronchiectasis, pneumonia.
In recent years, global warming, environmental pollution is serious, and PM2.5 exceeds standard, and haze is wreaked havoc, in addition virus, the spreading of antibacterial, and respiratory system disease that crowd suffers from increases severely, and especially coughs with anemopyretic, irritable cough is for work.Epidemic febrile disease from the beginning of, evil to defend point, defending gas strongly fragrant, folding mistake department, thus generate heat, micro evil wind is cold, lossless or have antiperspirant not smooth; Lung position is the highest and have one's ideas straightened out in nose, evil enters from mouth and nose, on violate in lung, Lung Qi obstraction, be then shown in cough; Wind heat is fought stagnation of QI blood, pents up into poison, and pyretic toxicity invasion and attack lung system door, be then shown in red swelling and pain of throat; Pathogenic warmth impairment of body fluid, thus thirsty; Red tip of the tongue, white and thin fur or micro-Huang, floating and rapid pulse be epidemic febrile disease from the beginning of evidence.Control the table that should pungently cool completely, heat-clearing and toxic substances removing.
Postinfectious cough is very common disease, mainly because of pathogenic factor in the exterior not to the greatest extent, discomfort in the throat, impaired depurative descending of lung QI, QI rising in reverse order is for caused by coughing.How because flu mistake is controlled wrong treatment, taken good care of the reasons such as improper or ferritic is weak, cause the course of disease delay, now cold, hot heresy has been gone in eight or nine cases out of ten, and only ailment said due to cold or exposure is reluctant to leave, and controls to detoxify by wind and heat dispersing, dispersing lung-QI and dissipating phlegm cough-relieving.In flu process, take high amount of drug, particularly antibiotic, easily cause gastrointestinal bacterial flora to lack of proper care, and loss of appetite, so just cause immunity of organisms to decline, be unfavorable for that body recovery is healthy.
Chinese medicine decoction is the organic conception using theory of Chinese medical science, determination for the treatment of based on pathogenesis obtained through differentiation of symptoms and signs, determined curative effect.But the mouthfeel of Chinese medicine decoction is pained, most people is difficult to accept, and is particularly more difficult to when patient's loss of appetite swallow.And the imbalance of intestinal microbial population, makes drug absorption weaken, and even cannot absorb.
Summary of the invention
Herb fermenting technological development original new drug is utilized to become the new trend of world today's medicament research and development, the study hotspot of international medical academia.Herb fermenting technology is on the basis of inheriting science of Chinese drug processing fermentation method, draws Microecology achievement, the high-tech herbal pharmaceutical new technique formed in conjunction with Modern microbiological engineering.
For overcoming above deficiency, the present invention, on the basis of Chinese medicine decoction, uses probiotics fermention technology, prepares a kind of fermented type Xuanfei Zhike mixture.
Technical scheme of the present invention is as follows:
A kind of probiotics fermentation-type Xuanfei Zhike mixture, obtained by probiotics fermention Chinese medicine compound, the composition of described Chinese medicine compound and content are: contain in every 1000ml probiotics fermentation-type Xuanfei Zhike mixture: Herba Schizonepetae 16 ~ 36g, the sweet Radix Stemonae 12 ~ 36g, Radix Cynanchi Paniculati 12 ~ 36g, Herba Houttuyniae 24 ~ 64g, Fructus Trichosanthis 24 ~ 64g, Fructus Forsythiae 20 ~ 48g, Flos Lonicerae 20 ~ 48g, Fructus Arctii (parched) 12 ~ 36g, Bulbus Fritillariae Thunbergii 12 ~ 36g, the Radix Paeoniae Alba 20 ~ 48g, honey-made mulberry bark 16 ~ 32g, sweet Radix Asteris 16 ~ 32g, sweet Flos Farfarae 16 ~ 32g, Radix Glehniae 12 ~ 28g, Radix Glycyrrhizae 4 ~ 16g; Above-mentioned Chinese medicine compound decocts with water, concentrated after, add or do not add glucose, yeast powder, yeast extract and peptone, adding inorganic salt, add or do not add sucrose and sucralose, obtaining via probiotics fermention.
Described probiotic bacteria is bacillus bifidus and lactobacillus.
A kind of preferred scheme, the composition of described Chinese medicine compound and content are: contain in every 1000ml probiotics fermentation-type Xuanfei Zhike mixture: Herba Schizonepetae 24g, sweet Radix Stemonae 24g, Radix Cynanchi Paniculati 24g, Herba Houttuyniae 48g, Fructus Trichosanthis 48g, Fructus Forsythiae 36g, Flos Lonicerae 36g, Fructus Arctii (parched) 20g, Bulbus Fritillariae Thunbergii 20g, Radix Paeoniae Alba 36g, honey-made mulberry bark 24g, sweet Radix Asteris 24g, sweet Flos Farfarae 24g, Radix Glehniae 20g, Radix Glycyrrhizae 8g.
The preparation method step of described probiotics fermentation-type Xuanfei Zhike mixture is as follows:
A. get 15 taste medicines in Chinese medicine compound, decoct with water secondary, 1.0 ~ 2.0 hours first times, second time 1.0 ~ 2.0 hours, collecting decoction, filter, measuring relative density at filtrate reduced in volume to 50 DEG C is 1.01 ~ 1.10;
B. above-mentioned concentrated solution add percent by volume be 0 ~ 1.5% glucose, the percent by volume yeast powder that is 0 ~ 0.2%, the percent by volume yeast extract that is 0 ~ 0.4%, percent by volume be 0 ~ 0.5% peptone, inorganic salt (FeSO
47H
2o1.0mg/100ml, ZnSO
47H
2o4.4mg/100ml, MgSO
47H
2o70mg/100ml, KH
2pO
4100mg/100ml, K
2hPO
43H
2o100mg/100ml), percent by volume is sucrose, the percent by volume of 0 ~ 10% is the sucralose of 0 ~ 0.03%, adjustment pH7.0 ± 0.5, and sterilizing 30 ~ 60 minutes at 110 ~ 121 DEG C, when temperature is down to 39 DEG C, inoculate the bacterium liquid of a kind of of pre-incubated percent by volume each 0.2 ~ 1.0% or two kinds of bacillus bifiduss, cellar culture 2 ~ 6 hours, inoculate the bacterium liquid of two kinds or three kinds lactobacilluss of pre-incubated percent by volume each 0.2 ~ 1.0%, then continue fermentation 12 ~ 42 hours, terminate fermentation when pH is down to below 4.2;
C. stabilizing treatment, aseptic subpackaged; Centrifugal or filter or Cryoprecipitation, add or do not add antiseptic, add purified water standardize solution, subpackage, sterilizing, obtain described probiotics fermentation-type Xuanfei Zhike mixture;
The mode of the inoculation described in preparation method step b also can be the bacterium liquid of the bacterium liquid of a kind of of the pre-incubated percent by volume of Simultaneous vaccination each 0.2 ~ 1.0% or two kinds of bacillus bifiduss and two kinds or three kinds lactobacilluss of pre-incubated percent by volume each 0.2 ~ 1.0%; The time of described fermentation is normal fermentation 16 ~ 48 hours, terminates fermentation when pH is down to below 4.2.
Bacillus bifidus described in preparation method step b is selected from bifidobacterium bifidum, bifidobacterium breve, bifidobacterium longum, animal bifidobacteria, bifidobacterium adolescentis and bifidobacteria infantis; Described lactobacillus is selected from bacillus acidophilus, Deshi Lactobacillus, Lactobacillus plantarum, Lactobacillus crispatus, lactobacillus helveticus, Lactobacillus salivarius, form lactobacillus, Lactobacillus johnsonii, Lactobacillus paracasei, Lactobacillus fermenti, lactobacillus casei and lactobacillus rhamnosus.
Strain derives from China typical culture collection center (CCTCC) (China, Wuhan) or China General Microbiological culture presevation administrative center (CGMCC) (China, Beijing) or be separated from the feces or intestinal submucosa tissue of normal person and obtain these bacterial isolateses.
Chinese medicine compound of the present invention uses the organic conception of theory of Chinese medical science, determination for the treatment of based on pathogenesis obtained through differentiation of symptoms and signs, wherein with Herba Schizonepetae, Radix Stemonae (processed), Radix Cynanchi Paniculati for monarch drug, the effect of three tastes long memorial wind, lung qi dispersing, cough-relieving altogether.Fructus Arctii, Flos Lonicerae, Fructus Forsythiae, Herba Houttuyniae, principal drug assistance dispelling wind, lung qi dispersing, cough-relieving are held concurrently heat-clearing and toxic substances removing, and Fructus Trichosanthis, Bulbus Fritillariae Thunbergii removing heat-phlegm, upper Six-element is the effect of ministerial drug long memorial wind cough-relieving altogether, dispersing lung-QI and dissipating phlegm.Honey Radix Asteris, sweet Flos Farfarae, honey-made mulberry bark nourishing the lung to keep the adverse QI downward, eliminating the pathogens from the lung cough-relieving, Radix Glehniae clearing lung and nourishing yin, can anti-wind heat heresy consumption impairing the lung the moon, can help again the pungent using warming therapy of Radix Cynanchi Paniculati processed, the cough-relieving of Radix Paeoniae Alba emergency, the upper five tastes are adjuvant drug altogether.Radix Glycyrrhizae coordinating the actions of various ingredients in a prescription, emergency of eliminating the phlegm is for making medicine.
The probiotic bacteria used in the present invention is bacillus bifidus and lactobacillus, and wherein bacillus bifidus is most important physiological antibacterial in people or mammalian body, is mainly distributed in small intestinal bottom and colonic.Lactobacillus is people's distribution in vivo probiotic bacteria the most widely, and in the gastrointestinal tract, this antibacterial is mainly distributed in widely different intestines.The present invention selects the common probiotics strain such as bacillus bifidus, lactobacillus, the overall popularity mainly distributed in human or animal's intestinal based on them, and the complementarity of their physiologically actives.
The present invention utilizes the antibacterial useful to human body, fermented tcm water extract in vitro.On the one hand, in accordance with Chinese medical theory, remain the water extract whole part of Chinese medicine, maintain the characteristic of Chinese medicine decoction; On the other hand, probiotic bacteria produces a large amount of enzyme in growth and breeding process, and Chinese medicine extract is through probiotics fermention, and some macromolecular substances are converted in vitro, are easy to absorption of human body, heighten the effect of a treatment; And lactobacillus during the fermentation, produce lactein, effectively can suppress the growth of pathogen.The similar antibiotic of effect of lactein, but mechanism of action is different, lactein mechanism of action to be first adsorbed on the cell membrane of object bacteria, to invade in film again and form penetrating duct, to cause important substance in born of the same parents, as the losses such as ATP, K+ or biochemical reaction obstacle, and cause Bacteria Indicators dead.Tool specificity, does not develop immunity to drugs completely and toxicity, and is not easily destroyed by small intestinal trypsin; Further, probiotic bacteria during the fermentation, produces and enriches flavor substance, improve the mouthfeel of Chinese medicine.
In general, the qf oral administration dosage of fermented type Xuanfei Zhike mixture of the present invention can according to the character of disease to be treated or pathological state, the order of severity, the age of patient, body weight, general health state, and patient is to sensitivity, the factor such as toleration and administering mode of medicine used, is determined by clinician according to the principle of individuation.
Through clinical proof, probiotics fermentation-type Xuanfei Zhike mixture of the present invention has significant curative effect to cough, and patient is easy to accept.
The probiotics fermention Chinese medicine with wind and heat dispersing removing toxic substances, dispersing lung-QI and dissipating phlegm cough-relieving effect provided by the invention, therapeutic effect is remarkable, and have no side effect, mouthfeel is good, all-ages.
Detailed description of the invention
Embodiment 1: the preparation of probiotics fermentation-type Xuanfei Zhike mixture A
Press column weight amount and take each Chinese medicine ingredients: Herba Schizonepetae 24g, sweet Radix Stemonae 24g, Radix Cynanchi Paniculati 24g, Herba Houttuyniae 48g, Fructus Trichosanthis 48g Fructus Forsythiae 36g, Flos Lonicerae 36g, Fructus Arctii (parched) 20g, Bulbus Fritillariae Thunbergii 20g, Radix Paeoniae Alba 36g, honey-made mulberry bark 24g, sweet Radix Asteris 24g, sweet Flos Farfarae 24g, Radix Glehniae 20g, Radix Glycyrrhizae 8g.
Above 15 tastes, decoct with water secondary, 1.5 hours first times, second time is 1.0 hours, collecting decoction, filter, measuring relative density at filtrate reduced in volume to 50 DEG C is 1.02 ~ 1.08, add percent by volume be 0.7% glucose, percent by volume be 0.1% yeast powder, inorganic salt (FeSO
47H
2o1.0mg/100ml, ZnSO
47H
2o4.4mg/100ml, MgSO
47H
2o70mg/100ml, KH
2pO
4100mg/100ml, K
2hPO
43H
2o100mg/100ml), percent by volume is the sucrose of 3.0%, percent by volume is the sucralose of 0.015%, adjustment pH6.8, be settled to 1000ml, and sterilizing after 40 minutes at 115 DEG C, when temperature is down to about 39 DEG C, inoculate bifidobacterium bifidum (normal human's separation of pre-incubated percent by volume each 0.5%, through Institute of Microorganism, Academia Sinica's qualification) and the bacterium liquid of bifidobacterium breve (AS1.2213), cultivate after about 6 hours for 37 DEG C, inoculate the bacillus acidophilus (AS1.1854) of percent by volume each 0.5%, the bacterium liquid of Lactobacillus plantarum (AS1.19) and Deshi Lactobacillus (AS1.1480), 37 DEG C of fermentations detect pH value in 16 hours, fermentation is terminated when pH value is down to below 4.2, stabilizing treatment, aseptic subpackaged, obtain.
Embodiment 2: the preparation of probiotics fermentation-type Xuanfei Zhike mixture B
Press column weight amount and take each Chinese medicine ingredients: Herba Schizonepetae 36g, sweet Radix Stemonae 12g, Radix Cynanchi Paniculati 12g, Herba Houttuyniae 64g, Fructus Trichosanthis 24g, Fructus Forsythiae 20g, Flos Lonicerae 48g, Fructus Arctii (parched) 36g, Bulbus Fritillariae Thunbergii 12g, Radix Paeoniae Alba 48g, honey-made mulberry bark 32g, sweet Radix Asteris 16g, sweet Flos Farfarae 16g, Radix Glehniae 12g, Radix Glycyrrhizae 16g.
Above 15 tastes, decoct with water secondary, 1.0 hours first times, second time is 1.0 hours, collecting decoction, filter, measuring relative density at filtrate reduced in volume to 50 DEG C is 1.01 ~ 1.06, add percent by volume be 1.0% glucose, the percent by volume yeast powder that is 0.2%, the percent by volume yeast extract that is 0.2%, percent by volume be 0.5% peptone, inorganic salt (FeSO
47H
2o1.0mg/100ml, ZnSO
47H
2o4.4mg/100ml, MgSO
47H
2o70mg/100ml, KH
2pO
4100mg/100ml, K
2hPO
43H
2o), percent by volume is the sucrose of 10%, adjustment pH7.2, and sterilizing after 30 minutes at 121 DEG C, when temperature is down to about 39 DEG C, the pre-incubated percent by volume of Simultaneous vaccination is bacterium liquid and the percent by volume respectively lactobacillus helveticus (CICC22816) of about 0.3% and the bacterium liquid of Lactobacillus paracasei (CICC6236) of the bifidobacterium longum (CICC6212) of 1.0%, 37 DEG C of fermentations detect pH value in 24 hours, fermentation is terminated when pH value is down to below 4.2, stabilizing treatment, filter, filtrate adding water is settled to 1000 milliliters, subpackage, sterilizing, to obtain final product.
Embodiment 3: the preparation of probiotics fermentation-type Xuanfei Zhike mixture C
Press column weight amount and take each Chinese medicine ingredients: Herba Schizonepetae 16g, sweet Radix Stemonae 36g, Radix Cynanchi Paniculati 36g, Herba Houttuyniae 24g, Fructus Trichosanthis 64g, Fructus Forsythiae 48g, Flos Lonicerae 24g, Fructus Arctii (parched) 12g, Bulbus Fritillariae Thunbergii 36g, Radix Paeoniae Alba 20g, honey-made mulberry bark 16g, sweet Radix Asteris 32g, sweet Flos Farfarae 32g, Radix Glehniae 28g, Radix Glycyrrhizae 4g.
Above 15 tastes, decoct with water secondary, 2.0 hours first times, and second time is 2.0 hours, collecting decoction, filter, and measuring relative density at filtrate reduced in volume to 50 DEG C is 1.03 ~ 1.10, adds inorganic salt (FeSO
47H
2o1.0mg/100ml, ZnSO
47H
2o4.4mg/100ml, MgSO
47H
2o70mg/100ml, KH
2pO
4100mg/100ml, K
2hPO
43H
2o), percent by volume is the sucralose of 0.03%, adjustment pH7.5, and sterilizing after 60 minutes at 110 DEG C, when temperature is down to about 39 DEG C, inoculate the animal bifidobacteria (CICC6165) that pre-incubated percent by volume is respectively 1.0%, the bacterium liquid of bifidobacterium adolescentis (CICC6176), cultivate after about 2 hours for 37 DEG C, inoculate each Lactobacillus salivarius (CICC23175) of about 0.5% of percent by volume and the bacterium liquid of lactobacillus rhamnosus (CICC6163), 37 DEG C of fermentations detect pH value in 30 hours, fermentation is terminated when pH value is down to below 4.2, stabilizing treatment, centrifugal, supernatant adds 0.2% sodium benzoate, add water and be settled to 1000 milliliters, subpackage, sterilizing, obtain.
Experimental example 1: clinical verification
Trial drug: make probiotics fermentation-type Xuanfei Zhike mixture A, B, C that specification is 250ml/ bottle respectively by embodiment 1, embodiment 2, embodiment 3, and by the Chinese medicine compound decoction of above formula preparation without probiotics fermention, be concentrated into same concentrations respectively, making specification is 250ml/ bottle, as control sample decoction a, b, the c of not fermenting.
Instructions of taking: oral, every day 1 bottle, divides and takes for 2 times.Within one week, be a course for the treatment of, a course for the treatment of is that one-period observes indices.
1, observation index
The tcm symptom such as to cough, cough up phlegm;
2, curative effect determinate standard
Formulate with reference to " new Chinese medicine guideline of clinical investigations " and State Administration of Traditional Chinese Medicine's " disease of tcm Standardization of diagnosis and curative effect ".Therapeutic index (n)=(before treating the rear integration of integration-treatment)/treat front integration × 100%.
3, clinical efficacy judges
Recovery from illness: cough and clinical symptom disappearance thereof, n=100%; Effective: cough and clinical symptoms thereof obviously alleviate, 70%≤n < 100%; Effective: cough and clinical symptoms thereof take a turn for the better, 30%≤n < 70%; Invalid: cough and clinical symptoms without change, n < 30%.
4, individual event symptom efficacy evaluation
Recovery from illness: transference cure; Effective: symptom reduces 2 grades (+++ →+); Effective: symptom reduce 1 grade (+++ → ++ or ++ →+); Invalid: symptom is without improvement.
5, result
All cases is out-patient, actual enter to organize case 120 example, wherein man 56 example, female 64 example; 39.5 years old mean age; Average course of disease 3.38 days; 24 examples that the state of an illness is slight, moderate 68 example, severe 28 example.Case is divided into groups, and according to check result, the state of an illness and tcm syndrome similar person mean allocation are in same prescription fermented type mixture group and do not ferment in decoction group, make case have comparability as far as possible.
Clinical efficacy: in table 1,2,3.
Table 1 fermented type Xuanfei Zhike mixture treats curative effect comparision table with the decoction that do not ferment
TCM syndrome curative effect before and after the treatment of table 2 probiotics fermentation-type Xuanfei Zhike mixture
Table 3 does not ferment and contrasts TCM syndrome curative effect before and after decoction treatment
As can be seen from result shown in table 1 ~ 3, fermented type Xuanfei Zhike mixture provided by the invention is evident in efficacy, compares curative effect significantly improve with unleavened identical prescription decoction.Illustrate that preparation method involved in the present invention improves the clinical efficacy of involved Chinese medicine compound in the present invention.
Claims (5)
1. a probiotics fermentation-type Xuanfei Zhike mixture, it is characterized in that, obtained by probiotics fermention Chinese medicine compound, the composition of described Chinese medicine compound and content are: contain in every 1000ml probiotics fermentation-type Xuanfei Zhike mixture: Herba Schizonepetae 16 ~ 36g, the honey Radix Stemonae 12 ~ 36g, Radix Cynanchi Paniculati 12 ~ 36g, Herba Houttuyniae 24 ~ 64g, Fructus Trichosanthis 24 ~ 64g, Fructus Forsythiae 20 ~ 48g, Flos Lonicerae 20 ~ 48g, Fructus Arctii (parched) 12 ~ 36g, Bulbus Fritillariae Thunbergii 12 ~ 36g, the Radix Paeoniae Alba 20 ~ 48g, honey-made mulberry bark 16 ~ 32g, honey Radix Asteris 16 ~ 32g, honey Flos Farfarae 16 ~ 32g, Radix Glehniae 12 ~ 28g, Radix Glycyrrhizae 4 ~ 16g, above-mentioned Chinese medicine compound decocts with water, concentrated after, add or do not add glucose, yeast powder, yeast extract and peptone, adding inorganic salt, add or do not add sucrose and sucralose, obtaining via probiotics fermention,
Described probiotic bacteria is bacillus bifidus and lactobacillus.
2. probiotics fermentation-type Xuanfei Zhike mixture according to claim 1, it is characterized in that, the composition of described Chinese medicine compound and content are: contain in every 1000ml probiotics fermentation-type Xuanfei Zhike mixture: Herba Schizonepetae 24g, sweet Radix Stemonae 24g, Radix Cynanchi Paniculati 24g, Herba Houttuyniae 48g, Fructus Trichosanthis 48g, Fructus Forsythiae 36g, Flos Lonicerae 36g, Fructus Arctii (parched) 20g, Bulbus Fritillariae Thunbergii 20g, Radix Paeoniae Alba 36g, honey-made mulberry bark 24g, sweet Radix Asteris 24g, sweet Flos Farfarae 24g, Radix Glehniae 20g, Radix Glycyrrhizae 8g.
3. the preparation method of the probiotics fermentation-type Xuanfei Zhike mixture described in claim 1 or 2, is characterized in that, preparation method step is as follows:
A. get 15 taste medicines in Chinese medicine compound, decoct with water secondary, 1.0 ~ 2.0 hours first times, second time 1.0 ~ 2.0 hours, collecting decoction, filter, measuring relative density at filtrate reduced in volume to 50 DEG C is 1.01 ~ 1.10, obtains concentrated solution;
B. the concentrated solution that step a obtains adds the glucose that percent by volume is 0 ~ 1.5%, percent by volume is the yeast powder of 0 ~ 0.2%, percent by volume is the yeast extract of 0 ~ 0.4%, percent by volume is the peptone of 0 ~ 0.5%, inorganic salt, percent by volume is the sucrose of 0 ~ 10%, percent by volume is the sucralose of 0 ~ 0.03%, adjusted to ph 7.0 ± 0.5, and sterilizing 30 ~ 60 minutes at 110 ~ 121 DEG C, when temperature is down to 39 DEG C, inoculate the bacterium liquid of a kind of of pre-incubated percent by volume each 0.2 ~ 1.0% or two kinds of bacillus bifiduss, cellar culture 2 ~ 6 hours, inoculate the bacterium liquid of two kinds or three kinds lactobacilluss of pre-incubated percent by volume each 0.2 ~ 1.0%, then fermentation 12 ~ 42 hours is continued, fermentation is terminated when pH is down to below 4.2,
C. stabilizing treatment, aseptic subpackaged; Centrifugal or filter or Cryoprecipitation, add or do not add antiseptic, add purified water standardize solution, subpackage, sterilizing, obtain described probiotics fermentation-type Xuanfei Zhike mixture;
Inorganic salt described in step b is: FeSO
47H
2o1.0mg/100ml, ZnSO
47H
2o4.4mg/100ml, MgSO
47H
2o70mg/100ml, KH
2pO
4100mg/100ml, K
2hPO
43H
2o100mg/100ml.
4. the preparation method of probiotics fermentation-type Xuanfei Zhike mixture according to claim 3, it is characterized in that, the mode of the inoculation described in preparation method step b is the bacterium liquid of the bacterium liquid of a kind of of the pre-incubated percent by volume of Simultaneous vaccination each 0.2 ~ 1.0% or two kinds of bacillus bifiduss and two kinds or three kinds lactobacilluss of pre-incubated percent by volume each 0.2 ~ 1.0%; The time of described fermentation is normal fermentation 16 ~ 48 hours, terminates fermentation when pH is down to below 4.2.
5. the preparation method of the probiotics fermentation-type Xuanfei Zhike mixture according to claim 3 or 4, it is characterized in that, the bacillus bifidus described in step b is selected from bifidobacterium bifidum, bifidobacterium breve, bifidobacterium longum, animal bifidobacteria, bifidobacterium adolescentis and bifidobacteria infantis; Described lactobacillus is selected from bacillus acidophilus, Deshi Lactobacillus, Lactobacillus plantarum, Lactobacillus crispatus, lactobacillus helveticus, Lactobacillus salivarius, form lactobacillus, Lactobacillus johnsonii, Lactobacillus paracasei, Lactobacillus fermenti, lactobacillus casei and lactobacillus rhamnosus.
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| CN109350661B (en) * | 2018-12-06 | 2021-05-04 | 山东宝来利来生物工程股份有限公司 | Fermented traditional Chinese medicine composition for relieving cough and reducing sputum and application thereof in preventing and treating porcine respiratory diseases |
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| CN109350661B (en) * | 2018-12-06 | 2021-05-04 | 山东宝来利来生物工程股份有限公司 | Fermented traditional Chinese medicine composition for relieving cough and reducing sputum and application thereof in preventing and treating porcine respiratory diseases |
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