CN113100125B - Method for improving survival rate of coilia ectenes parents - Google Patents
Method for improving survival rate of coilia ectenes parents Download PDFInfo
- Publication number
- CN113100125B CN113100125B CN202110370869.5A CN202110370869A CN113100125B CN 113100125 B CN113100125 B CN 113100125B CN 202110370869 A CN202110370869 A CN 202110370869A CN 113100125 B CN113100125 B CN 113100125B
- Authority
- CN
- China
- Prior art keywords
- parents
- coilia ectenes
- coilia
- survival rate
- cultivation
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 241001460967 Coilia nasus Species 0.000 title claims abstract description 153
- 238000000034 method Methods 0.000 title claims abstract description 68
- 230000004083 survival effect Effects 0.000 title claims abstract description 51
- DHMQDGOQFOQNFH-UHFFFAOYSA-N Glycine Chemical compound NCC(O)=O DHMQDGOQFOQNFH-UHFFFAOYSA-N 0.000 claims abstract description 136
- 239000004471 Glycine Substances 0.000 claims abstract description 72
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 claims abstract description 69
- 150000002148 esters Chemical class 0.000 claims abstract description 51
- 230000033228 biological regulation Effects 0.000 claims abstract description 23
- 235000003642 hunger Nutrition 0.000 claims abstract description 19
- 230000003750 conditioning effect Effects 0.000 claims abstract description 6
- 230000037351 starvation Effects 0.000 claims abstract description 3
- GHBFNMLVSPCDGN-UHFFFAOYSA-N rac-1-monooctanoylglycerol Chemical compound CCCCCCCC(=O)OCC(O)CO GHBFNMLVSPCDGN-UHFFFAOYSA-N 0.000 claims description 52
- 230000008569 process Effects 0.000 claims description 26
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 24
- 238000009395 breeding Methods 0.000 claims description 19
- 230000001488 breeding effect Effects 0.000 claims description 19
- 229940087068 glyceryl caprylate Drugs 0.000 claims description 18
- 239000003795 chemical substances by application Substances 0.000 claims description 11
- 230000001105 regulatory effect Effects 0.000 claims description 9
- 239000000126 substance Substances 0.000 claims description 3
- 230000009467 reduction Effects 0.000 abstract description 33
- 210000004185 liver Anatomy 0.000 abstract description 32
- 238000011534 incubation Methods 0.000 abstract description 10
- 238000009360 aquaculture Methods 0.000 abstract description 5
- 244000144974 aquaculture Species 0.000 abstract description 5
- FYSNRJHAOHDILO-UHFFFAOYSA-N thionyl chloride Chemical compound ClS(Cl)=O FYSNRJHAOHDILO-UHFFFAOYSA-N 0.000 description 44
- 230000000052 comparative effect Effects 0.000 description 29
- 241000851180 Palaemon carinicauda Species 0.000 description 24
- RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 description 21
- BGMYHTUCJVZIRP-UHFFFAOYSA-N Nojirimycin Natural products OCC1NC(O)C(O)C(O)C1O BGMYHTUCJVZIRP-UHFFFAOYSA-N 0.000 description 16
- 235000019820 disodium diphosphate Nutrition 0.000 description 16
- GYQBBRRVRKFJRG-UHFFFAOYSA-L disodium pyrophosphate Chemical group [Na+].[Na+].OP([O-])(=O)OP(O)([O-])=O GYQBBRRVRKFJRG-UHFFFAOYSA-L 0.000 description 16
- BGMYHTUCJVZIRP-GASJEMHNSA-N nojirimycin Chemical compound OC[C@H]1NC(O)[C@H](O)[C@@H](O)[C@@H]1O BGMYHTUCJVZIRP-GASJEMHNSA-N 0.000 description 16
- QBOIGWDDFIREJQ-UHFFFAOYSA-N 4,4-dimethoxybutan-2-ol Chemical compound COC(OC)CC(C)O QBOIGWDDFIREJQ-UHFFFAOYSA-N 0.000 description 15
- 238000006243 chemical reaction Methods 0.000 description 15
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 14
- 230000009278 visceral effect Effects 0.000 description 13
- 230000035882 stress Effects 0.000 description 12
- 238000012360 testing method Methods 0.000 description 12
- 241001200292 Mystus Species 0.000 description 10
- 238000010521 absorption reaction Methods 0.000 description 9
- 230000000694 effects Effects 0.000 description 8
- 235000013305 food Nutrition 0.000 description 8
- 238000002360 preparation method Methods 0.000 description 8
- IOVCWXUNBOPUCH-UHFFFAOYSA-M Nitrite anion Chemical compound [O-]N=O IOVCWXUNBOPUCH-UHFFFAOYSA-M 0.000 description 7
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 7
- 230000007613 environmental effect Effects 0.000 description 7
- 230000003203 everyday effect Effects 0.000 description 7
- -1 lipid peroxide Chemical class 0.000 description 7
- 229910052757 nitrogen Inorganic materials 0.000 description 7
- 239000001301 oxygen Substances 0.000 description 7
- 229910052760 oxygen Inorganic materials 0.000 description 7
- 238000010992 reflux Methods 0.000 description 7
- 239000013535 sea water Substances 0.000 description 7
- 238000000967 suction filtration Methods 0.000 description 7
- 241000251468 Actinopterygii Species 0.000 description 6
- 238000001816 cooling Methods 0.000 description 6
- 230000007423 decrease Effects 0.000 description 6
- 238000001035 drying Methods 0.000 description 6
- 235000019688 fish Nutrition 0.000 description 6
- 125000005456 glyceride group Chemical group 0.000 description 6
- 238000002156 mixing Methods 0.000 description 6
- 239000002689 soil Substances 0.000 description 6
- 238000005406 washing Methods 0.000 description 6
- 239000000203 mixture Substances 0.000 description 5
- 230000036542 oxidative stress Effects 0.000 description 5
- 239000002253 acid Substances 0.000 description 4
- 238000001514 detection method Methods 0.000 description 4
- WSMYVTOQOOLQHP-UHFFFAOYSA-N Malondialdehyde Chemical compound O=CCC=O WSMYVTOQOOLQHP-UHFFFAOYSA-N 0.000 description 3
- 229940118019 malondialdehyde Drugs 0.000 description 3
- 230000000116 mitigating effect Effects 0.000 description 3
- 238000010998 test method Methods 0.000 description 3
- 241000229375 Palaemon Species 0.000 description 2
- 238000002835 absorbance Methods 0.000 description 2
- 238000003556 assay Methods 0.000 description 2
- 230000005779 cell damage Effects 0.000 description 2
- 208000037887 cell injury Diseases 0.000 description 2
- 230000006870 function Effects 0.000 description 2
- 125000003630 glycyl group Chemical group [H]N([H])C([H])([H])C(*)=O 0.000 description 2
- 230000003204 osmotic effect Effects 0.000 description 2
- 210000001519 tissue Anatomy 0.000 description 2
- 206010002091 Anaesthesia Diseases 0.000 description 1
- 235000014161 Caesalpinia gilliesii Nutrition 0.000 description 1
- 244000003240 Caesalpinia gilliesii Species 0.000 description 1
- 241000252203 Clupea harengus Species 0.000 description 1
- 241001454694 Clupeiformes Species 0.000 description 1
- 241000825055 Coilia Species 0.000 description 1
- OAKJQQAXSVQMHS-UHFFFAOYSA-N Hydrazine Chemical compound NN OAKJQQAXSVQMHS-UHFFFAOYSA-N 0.000 description 1
- UFHFLCQGNIYNRP-UHFFFAOYSA-N Hydrogen Chemical compound [H][H] UFHFLCQGNIYNRP-UHFFFAOYSA-N 0.000 description 1
- LTIPUQSMGRSZOQ-UHFFFAOYSA-N [C].[C].[O] Chemical compound [C].[C].[O] LTIPUQSMGRSZOQ-UHFFFAOYSA-N 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 230000003044 adaptive effect Effects 0.000 description 1
- 210000000577 adipose tissue Anatomy 0.000 description 1
- 230000037005 anaesthesia Effects 0.000 description 1
- 235000019513 anchovy Nutrition 0.000 description 1
- 230000006851 antioxidant defense Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 230000006378 damage Effects 0.000 description 1
- 238000003912 environmental pollution Methods 0.000 description 1
- 235000019514 herring Nutrition 0.000 description 1
- 230000013632 homeostatic process Effects 0.000 description 1
- 239000001257 hydrogen Substances 0.000 description 1
- 229910052739 hydrogen Inorganic materials 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 238000002329 infrared spectrum Methods 0.000 description 1
- 230000006651 lactation Effects 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000006461 physiological response Effects 0.000 description 1
- 230000035755 proliferation Effects 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 230000011506 response to oxidative stress Effects 0.000 description 1
- 230000003938 response to stress Effects 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 230000036186 satiety Effects 0.000 description 1
- 235000019627 satiety Nutrition 0.000 description 1
- 230000019491 signal transduction Effects 0.000 description 1
- 230000017423 tissue regeneration Effects 0.000 description 1
- 210000001835 viscera Anatomy 0.000 description 1
Images
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
- A01K61/10—Culture of aquatic animals of fish
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
- A01K61/10—Culture of aquatic animals of fish
- A01K61/13—Prevention or treatment of fish diseases
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
- C07C227/00—Preparation of compounds containing amino and carboxyl groups bound to the same carbon skeleton
- C07C227/14—Preparation of compounds containing amino and carboxyl groups bound to the same carbon skeleton from compounds containing already amino and carboxyl groups or derivatives thereof
- C07C227/18—Preparation of compounds containing amino and carboxyl groups bound to the same carbon skeleton from compounds containing already amino and carboxyl groups or derivatives thereof by reactions involving amino or carboxyl groups, e.g. hydrolysis of esters or amides, by formation of halides, salts or esters
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
- C07C229/00—Compounds containing amino and carboxyl groups bound to the same carbon skeleton
- C07C229/02—Compounds containing amino and carboxyl groups bound to the same carbon skeleton having amino and carboxyl groups bound to acyclic carbon atoms of the same carbon skeleton
- C07C229/04—Compounds containing amino and carboxyl groups bound to the same carbon skeleton having amino and carboxyl groups bound to acyclic carbon atoms of the same carbon skeleton the carbon skeleton being acyclic and saturated
- C07C229/06—Compounds containing amino and carboxyl groups bound to the same carbon skeleton having amino and carboxyl groups bound to acyclic carbon atoms of the same carbon skeleton the carbon skeleton being acyclic and saturated having only one amino and one carboxyl group bound to the carbon skeleton
- C07C229/08—Compounds containing amino and carboxyl groups bound to the same carbon skeleton having amino and carboxyl groups bound to acyclic carbon atoms of the same carbon skeleton the carbon skeleton being acyclic and saturated having only one amino and one carboxyl group bound to the carbon skeleton the nitrogen atom of the amino group being further bound to hydrogen atoms
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Environmental Sciences (AREA)
- Marine Sciences & Fisheries (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention discloses a method for improving the survival rate of coilia ectenes parents, which belongs to the field of aquaculture and comprises the following steps: and (3) environment regulation and cultivation: firstly, carrying out starvation cultivation on the coilia ectenes parents, and then carrying out adjustment cultivation; the conditioning incubation used a conditioner containing glycine ester hydrochloride. The survival rate of the coilia ectenes parents is high, the survival rate of the coilia ectenes parents cultured by the method is more than 85%, and the survival rate of the coilia ectenes parents cultured by the method after spawning is more than 90%; the reduction rate of the dirty body ratio of the coilia ectenes parents is small, and the reduction rate of the dirty body ratio of the coilia ectenes parents is less than 20%; the reduction rate of the liver body ratio of the coilia ectenes parents is small, and the reduction rate of the liver body ratio of the coilia ectenes parents is below 28 percent; the content of MDA in the liver of the coilia ectenes parent is low, and the content of MDA in the liver of the coilia ectenes parent is less than 65 nmol/mL.
Description
Technical Field
The invention belongs to the field of aquaculture, and particularly relates to a method for improving the survival rate of coilia ectenes parents.
Background
Coilia ectenes belongs to the order of herring, anchovy, genus coilia, is slender and laterally flat, and is commonly called as coilia ectenes because the fish body gradually tapers backwards and becomes thin and sharp and is in a sickle shape. The coilia ectenes is widely distributed in China, exists in main sea rivers and sea areas including yellow sea, Bohai sea, east sea, Yangtze river, yellow river, sea river and the like, and is mainly distributed on the coast of the western pacific in the world. Coilia ectenes belongs to fishes migrating back to the river, is called as one of three delicacies in the Yangtze river from ancient times, and has extremely high economic and nutritional values. However, due to the problems of environmental pollution, over-fishing and the like in recent years, the natural population resources of the coilia ectenes are rapidly reduced and are threatened to be extinct. At present, artificial breeding and proliferation releasing are main effective measures for recovering endangered fish population resources. However, coilia ectenes is very sensitive to stress reaction, and is easy to be injured and die by manual operation, so that the artificial breeding process of coilia ectenes is obstructed. Therefore, the important current problem is to effectively regulate and relieve the stress response and improve the survival rate of the coilia ectenes. Stress, one of the normal physiological responses, has its adaptive significance with the aim of maintaining homeostasis. The action of stress on the body depends mainly on two aspects, namely the stress intensity and the self-ability to resist the stress. Proper stress can improve the ability of fish to adapt to the environment, but excessive stress can cause damage to fish.
Disclosure of Invention
The invention aims to provide glycine ester hydrochloride which can be used for aquaculture and can improve the survival rate of aquatic products.
The technical scheme adopted by the invention for realizing the purpose is as follows:
a glycine ester hydrochloride of the formula: HCl & NH 2 CH 2 COOCH(CH 3 )CH 2 CH(OCH 3 )OCH 3 。
The invention discloses application of glycine ester hydrochloride and glyceryl monocaprylate in aquaculture and/or aquaculture.
A process for the preparation of glycine ester hydrochloride comprising: glycine and 3-hydroxybutyraldehyde dimethyl acetal react in thionyl chloride to prepare glycine ester hydrochloride.
Preferably, in the preparation of the glycine ester hydrochloride, glycine and 3-hydroxybutyraldehyde dimethyl acetal are added into thionyl chloride, mixed for 10-90min, subjected to reflux reaction at 80-90 ℃ for 0.5-6h, naturally cooled after the reaction is finished, put into a refrigerator at 0-10 ℃ for 3-12h, subjected to suction filtration, washed with diethyl ether, and dried to obtain the glycine ester hydrochloride.
More preferably, glycine is added in an amount of 30-60 wt% of thionyl chloride.
More preferably, 3-hydroxybutyraldehyde dimethyl acetal is added in an amount of 50-100 wt% of thionyl chloride.
The invention aims to provide a method for improving the survival rate of coilia ectenes parents, which reduces the reduction rate of liver body ratio, the reduction rate of visceral body ratio and the MDA content in liver.
The technical scheme adopted by the invention for realizing the purpose is as follows:
a method for improving survival rate of coilia ectenes parents comprises the following steps:
and (3) environment regulation and cultivation: carrying out starvation cultivation on the coilia ectenes parents, and then carrying out adjustment cultivation; the conditioning medium containing glycine ester hydrochloride was used in the conditioning incubation.
Preferably, the modulators are glycine ester hydrochloride and glycerol monocaprylate. During hunger cultivation, the functions of tissue repair, osmotic pressure regulation and the like of the coilia ectenes parents are obviously influenced, lipid peroxide in the liver is decomposed to generate malondialdehyde with biotoxicity, the degree of cell damage is improved, an antioxidant defense system in the coilia ectenes is damaged, glycine ester hydrochloride and glyceryl monocaprylate are jointly used, the tissue of the coilia ectenes parents is repaired by the functions of osmotic pressure regulation and the like, the content of the malondialdehyde in the liver is reduced, the reduction rate of the liver-body ratio of the coilia ectenes parents is reduced, the reduction rate of the visceral-body ratio of the coilia ectenes parents is reduced, and the survival rate of the coilia ectenes parents is improved.
Preferably, the feeding amount in the adjusted cultivation is 5-60% of the satiety amount.
Preferably, the pH of the water body in the environment-controlled cultivation is adjusted to 6.5-8.
Preferably, glycine ester hydrochloride is used in the modulator in an amount of 10-100. mu.M.
Preferably, the amount of glyceryl monocaprylate used in the modulator is 1-100 mM.
Preferably, in the environment regulation and control cultivation, the coilia ectenes parents subjected to parent cultivation are subjected to hunger cultivation for 3-7d, the water temperature is kept at 22-25 ℃, a regulator is added into a cultivation pool after the hunger cultivation, seawater is added for regulating the salinity to 5-10, mysorethorn and palaemon carinicauda are fed, the preferable amount of food is 5-60%, and the cultivation time is regulated to 5-10 d.
Preferably, the environment-controlled breeding comprises a parent breeding process before the breeding.
More preferably, in the parent cultivation, the coilia ectenes parents are placed in a cultivation pool, the stocking density is 20-40 tails/pool, the water temperature is kept at 22-25 ℃, the pH is adjusted to 6.5-8, the mysid and the palaemon carinicauda are fed every day, the feeding is preferably performed after the mystus and the palaemon carinicauda are fed, and the cultivation time is 5-10 days.
Preferably, the environmental conditioning incubation is followed by an intensive incubation process.
More preferably, a mitigating agent is used in the enhanced incubation.
More preferably, in the intensified cultivation, the coilia ectenes parents cultured by environment regulation are completely fished out by a net and exposed in the air for 5-30s, then the coilia ectenes parents are put back into the water for 270-295s, the coilia ectenes parents are repeatedly fished out and put back for 3-10 times, a release agent is added into a cultivation pool after stress treatment, the water temperature is kept at 22-25 ℃, the pH value is kept at 6.5-8, the mysid and the palaemon carinicauda are fed, the coilia ectenes and the palaemon carinicauda are preferably fed with full food, and the cultivation time is 5-10 d.
Still more preferably, the extinguisher is disodium dihydrogen pyrophosphate and 1-mannose nojirimycin hydrochloride, the disodium dihydrogen pyrophosphate is used in an amount of 1-10mM, and the 1-mannose nojirimycin hydrochloride is used in an amount of 10-100 mM. Disodium dihydrogen pyrophosphate and 1-mannose nojirimycin hydrochloride stimulate the formation and lactation of signal transduction tissues, adipose tissues and the like, regulate and control the stress adaptability of the coilia ectenes parents, reduce the content of malondialdehyde in livers, reduce cell damage and improve the survival rate of the coilia ectenes parents.
Preferably, the feeding is given 2 times a day, 7 am and 5 am.
Preferably, the concentration of dissolved oxygen is more than 6mg/L, the concentration of ammoniacal nitrogen is less than 0.01mg/L, and the concentration of nitrite is less than 0.01 mg/L.
The invention discloses application of glycine ester hydrochloride and glyceryl monocaprylate to reduction of MDA content in liver of coilia ectenes parent.
The invention improves the survival rate of the coilia ectenes parents by adopting the environment regulation and control cultivation containing the hunger cultivation process and the regulation cultivation process, thereby having the following beneficial effects: the survival rate of the coilia ectenes parents is high, the survival rate of the coilia ectenes parents cultured by the method is more than 85%, and the survival rate of the coilia ectenes parents cultured by the method after spawning is more than 90%; the reduction rate of the visceral volume ratio of the coilia ectenes parents is small, and the reduction rate of the visceral volume ratio of the coilia ectenes parents is below 20%; the reduction rate of the liver body ratio of the coilia ectenes parents is small, and the reduction rate of the liver body ratio of the coilia ectenes parents is below 28 percent; the content of MDA in the liver of the coilia ectenes parent is low, and the content of MDA in the liver of the coilia ectenes parent is less than 65 nmol/mL. Therefore, the method for improving the survival rate of the coilia ectenes parents can reduce the reduction rate of the liver-body ratio, the reduction rate of the visceral-body ratio and the MDA content in the liver.
Drawings
FIG. 1 is an infrared image of glycine ester hydrochloride;
fig. 2 is a graph of survival rate of coilia ectenes parents;
figure 3 is a graph of the reduction rate of the visceral volume ratio of the coilia ectenes parents;
FIG. 4 is a graph of the rate of decline of the liver-to-body ratio of coilia ectenes parent;
fig. 5 is a graph of the content of MDA in the liver of coilia ectenes parent.
Detailed Description
The technical solution of the present invention is further described in detail below with reference to the following detailed description and the accompanying drawings:
example 1:
a method for improving the survival rate of coilia ectenes parents,
preparation of glycine ester hydrochloride ester: adding glycine and 3-hydroxybutyraldehyde dimethyl acetal into thionyl chloride, mixing for 30min, carrying out reflux reaction at the temperature of 80 ℃ for 4h, naturally cooling after the reaction is finished, putting the mixture into a refrigerator at the temperature of 5 ℃ for 8h, carrying out suction filtration, washing with diethyl ether, and drying to obtain glycine ester hydrochloride. The addition amount of glycine is 40 wt% of thionyl chloride, and the addition amount of 3-hydroxybutyraldehyde dimethyl acetal is 80 wt% of thionyl chloride.
Parent breeding: putting the coilia ectenes parents into a culture pond, keeping the stocking density at 30 tails/pond, keeping the water temperature at 25 ℃, adjusting the pH value to 7.2, feeding mysid and palaemon carinicauda every day, preferably feeding mystus fuscus and palaemon carinicauda for 7 days.
And (3) environment regulation and cultivation: starving coilia ectenes parents cultured by parents for 5 days, keeping the water temperature at 25 ℃ and the pH at 7.2, adding a regulator into a culture pond after starving culture, adding seawater to regulate the salinity to 10, and feeding mystus fuscus and palaemon carinicauda, wherein the full food capacity is preferably 50%, and the culture time is regulated to 5 days. The regulator comprises glycine ester hydrochloride and monocaprylic glyceride, wherein the usage amount of the glycine ester hydrochloride is 60 mu M, and the usage amount of the monocaprylic glyceride is 20 mM.
The concentration of dissolved oxygen is controlled to be 8.5mg/L, the concentration of ammoniacal nitrogen is controlled to be less than 0.01mg/L, and the concentration of nitrite is controlled to be less than 0.01mg/L in the cultivation process.
The breeding process comprises feeding 2 times a day, 7 points in the morning and 5 points in the evening.
Soil absorption is carried out once a day in the cultivation process.
Example 2:
a method for improving the survival rate of coilia ectenes parents,
preparation of glycine ester hydrochloride ester: adding glycine and 3-hydroxybutyraldehyde dimethyl acetal into thionyl chloride, mixing for 30min, carrying out reflux reaction at the temperature of 80 ℃ for 4h, naturally cooling after the reaction is finished, putting the cooled product into a refrigerator at the temperature of 5 ℃ for 8h, carrying out suction filtration, washing with diethyl ether, and drying to obtain glycine ester hydrochloride. The addition amount of glycine is 40 wt% of thionyl chloride, and the addition amount of 3-hydroxybutyraldehyde dimethyl acetal is 80 wt% of thionyl chloride.
Parent breeding: putting the coilia ectenes parents into a culture pond, keeping the stocking density at 30 tails/pond, keeping the water temperature at 25 ℃, adjusting the pH value to 7.2, feeding mysid and palaemon carinicauda every day, preferably feeding mystus fuscus and palaemon carinicauda for 7 days.
And (3) environment regulation and cultivation: starving coilia ectenes parents cultured by parents for 5 days, keeping the water temperature at 25 ℃ and the pH at 7.2, adding a regulator into a culture pond after starving culture, adding seawater to regulate the salinity to 10, and feeding mystus fuscus and palaemon carinicauda, wherein the full food capacity is preferably 50%, and the culture time is regulated to 5 days. The regulator comprises glycine ester hydrochloride and glycerol monocaprylate, wherein the usage amount of the glycine ester hydrochloride is 60 mu M, and the usage amount of the glycerol monocaprylate is 40 mM.
The concentration of dissolved oxygen is controlled to be 8.5mg/L, the concentration of ammoniacal nitrogen is controlled to be less than 0.01mg/L, and the concentration of nitrite is controlled to be less than 0.01mg/L in the cultivation process.
The breeding process comprises feeding 2 times a day, 7 points in the morning and 5 points in the evening.
Soil absorption is carried out once a day in the cultivation process.
Example 3:
a method for improving the survival rate of coilia ectenes parents,
preparation of glycine ester hydrochloride ester: adding glycine and 3-hydroxybutyraldehyde dimethyl acetal into thionyl chloride, mixing for 30min, carrying out reflux reaction at the temperature of 80 ℃ for 4h, naturally cooling after the reaction is finished, putting the mixture into a refrigerator at the temperature of 5 ℃ for 8h, carrying out suction filtration, washing with diethyl ether, and drying to obtain glycine ester hydrochloride. The addition amount of glycine is 40 wt% of thionyl chloride, and the addition amount of 3-hydroxybutyraldehyde dimethyl acetal is 80 wt% of thionyl chloride.
Parent breeding: putting the coilia ectenes parents into a culture pond, keeping the stocking density at 30 tails/pond, keeping the water temperature at 25 ℃, adjusting the pH value to 7.2, and feeding mysid and palaemon carinicauda every day for 7 days with the preferable feeding time being 7 days.
And (3) environment regulation and cultivation: starving coilia ectenes parents cultured by parents for 5 days, keeping the water temperature at 25 ℃ and the pH at 7.2, adding a regulator into a culture pond after starving culture, adding seawater to regulate the salinity to 10, and feeding mystus fuscus and palaemon carinicauda, wherein the full food capacity is preferably 50%, and the culture time is regulated to 5 days. The regulator comprises glycine ester hydrochloride and glycerol monocaprylate, wherein the usage amount of the glycine ester hydrochloride is 60 mu M, and the usage amount of the glycerol monocaprylate is 70 mM.
The concentration of dissolved oxygen is controlled to be 8.5mg/L, the concentration of ammoniacal nitrogen is controlled to be less than 0.01mg/L, and the concentration of nitrite is controlled to be less than 0.01mg/L in the cultivation process.
The breeding process comprises feeding 2 times a day, 7 points in the morning and 5 points in the evening.
Soil absorption is carried out once a day in the cultivation process.
Example 4:
a method for improving the survival rate of coilia ectenes parents,
preparation of glycine ester hydrochloride ester: adding glycine and 3-hydroxybutyraldehyde dimethyl acetal into thionyl chloride, mixing for 30min, carrying out reflux reaction at the temperature of 80 ℃ for 4h, naturally cooling after the reaction is finished, putting the mixture into a refrigerator at the temperature of 5 ℃ for 8h, carrying out suction filtration, washing with diethyl ether, and drying to obtain glycine ester hydrochloride. The addition amount of glycine is 40 wt% of thionyl chloride, and the addition amount of 3-hydroxybutyraldehyde dimethyl acetal is 80 wt% of thionyl chloride.
Parent breeding: putting the coilia ectenes parents into a culture pond, keeping the stocking density at 30 tails/pond, keeping the water temperature at 25 ℃, adjusting the pH value to 7.2, and feeding mysid and palaemon carinicauda every day for 7 days with the preferable feeding time being 7 days.
And (3) environment regulation and cultivation: starving coilia ectenes parents cultured by parents for 5 days, keeping the water temperature at 25 ℃ and the pH at 7.2, adding a regulator into a culture pond after starving culture, adding seawater to regulate the salinity to 10, and feeding mystus fuscus and palaemon carinicauda, wherein the full food capacity is preferably 50%, and the culture time is regulated to 5 days. The regulator is glycine ester hydrochloride and monocaprylic acid glyceride, the using amount of the glycine ester hydrochloride is 60 mu M, and the using amount of the monocaprylic acid glyceride is 70 mM.
Reinforced cultivation: fishing out all coilia ectenes parents cultured by environment regulation by using a net, exposing the coilia ectenes parents in the air for 15s, then putting the coilia ectenes parents in water for 285s, repeatedly fishing out and putting back for 10 times, adding a release agent into a culturing pool after stress treatment, keeping the water temperature at 25 ℃, keeping the pH at 7.2, and feeding mysid and palaemon carinicauda for 7d, wherein the coilia ectenes and the palaemon carinicauda are preferably eaten fully. The relieving agent is disodium dihydrogen pyrophosphate and 1-mannose nojirimycin hydrochloride, the usage amount of the disodium dihydrogen pyrophosphate is 2mM, and the usage amount of the 1-mannose nojirimycin hydrochloride is 30 mM.
The concentration of dissolved oxygen is controlled to be 8.5mg/L, the concentration of ammoniacal nitrogen is controlled to be less than 0.01mg/L, and the concentration of nitrite is controlled to be less than 0.01mg/L in the cultivation process.
The breeding process comprises feeding 2 times a day, 7 points in the morning and 5 points in the evening.
Soil absorption is carried out once a day in the cultivation process.
Example 5:
a method for improving the survival rate of coilia ectenes parents,
preparation of glycine ester hydrochloride ester: adding glycine and 3-hydroxybutyraldehyde dimethyl acetal into thionyl chloride, mixing for 30min, carrying out reflux reaction at the temperature of 80 ℃ for 4h, naturally cooling after the reaction is finished, putting the mixture into a refrigerator at the temperature of 5 ℃ for 8h, carrying out suction filtration, washing with diethyl ether, and drying to obtain glycine ester hydrochloride. The addition amount of glycine is 40 wt% of thionyl chloride, and the addition amount of 3-hydroxybutyraldehyde dimethyl acetal is 80 wt% of thionyl chloride.
Parent breeding: putting the coilia ectenes parents into a culture pond, keeping the stocking density at 30 tails/pond, keeping the water temperature at 25 ℃, adjusting the pH value to 7.2, and feeding mysid and palaemon carinicauda every day for 7 days with the preferable feeding time being 7 days.
And (3) environment regulation and cultivation: starving coilia ectenes parents cultured by parents for 5 days, keeping the water temperature at 25 ℃ and the pH at 7.2, adding a regulator into a culture pond after starving culture, adding seawater to regulate the salinity to 10, and feeding mystus fuscus and palaemon carinicauda, wherein the full food capacity is preferably 50%, and the culture time is regulated to 5 days. The regulator comprises glycine ester hydrochloride and glycerol monocaprylate, wherein the usage amount of the glycine ester hydrochloride is 60 mu M, and the usage amount of the glycerol monocaprylate is 70 mM.
Reinforced cultivation: fishing out all coilia ectenes parents cultured by environment regulation by using a net, exposing the coilia ectenes parents in the air for 15s, then putting the coilia ectenes parents in water for 285s, repeatedly fishing out and putting back for 10 times, adding a release agent into a culture pond after stress treatment, keeping the water temperature at 25 ℃ and the pH at 7.2, and feeding mysid and palaemon carinicauda until the coilia ectenes and the palaemon carinicaa are fed well, wherein the culture time is 7 days. The relieving agent is disodium dihydrogen pyrophosphate and 1-mannose nojirimycin hydrochloride, the use amount of the disodium dihydrogen pyrophosphate is 4mM, and the use amount of the 1-mannose nojirimycin hydrochloride is 60 mM.
The concentration of dissolved oxygen is controlled to be 8.5mg/L, the concentration of ammoniacal nitrogen is controlled to be less than 0.01mg/L, and the concentration of nitrite is controlled to be less than 0.01mg/L in the cultivation process.
The breeding process comprises feeding 2 times a day, 7 points in the morning and 5 points in the evening.
Soil absorption is carried out once a day in the cultivation process.
Example 6:
a method for improving the survival rate of coilia ectenes parents,
preparation of glycine ester hydrochloride ester: adding glycine and 3-hydroxybutyraldehyde dimethyl acetal into thionyl chloride, mixing for 30min, carrying out reflux reaction at the temperature of 80 ℃ for 4h, naturally cooling after the reaction is finished, putting the mixture into a refrigerator at the temperature of 5 ℃ for 8h, carrying out suction filtration, washing with diethyl ether, and drying to obtain glycine ester hydrochloride. The addition amount of glycine is 40 wt% of thionyl chloride, and the addition amount of 3-hydroxybutyraldehyde dimethyl acetal is 80 wt% of thionyl chloride.
Parent breeding: putting the coilia ectenes parents into a culture pond, keeping the stocking density at 30 tails/pond, keeping the water temperature at 25 ℃, adjusting the pH value to 7.2, feeding mysid and palaemon carinicauda every day, preferably feeding mystus fuscus and palaemon carinicauda for 7 days.
And (3) environment regulation and cultivation: starving coilia ectenes parents cultured by parents for 5 days, keeping the water temperature at 25 ℃ and the pH at 7.2, adding a regulator into a culture pond after starving culture, adding seawater to regulate the salinity to 10, and feeding mystus fuscus and palaemon carinicauda, wherein the full food capacity is preferably 50%, and the culture time is regulated to 5 days. The regulator is glycine ester hydrochloride and monocaprylic acid glyceride, the using amount of the glycine ester hydrochloride is 60 mu M, and the using amount of the monocaprylic acid glyceride is 70 mM.
Reinforced cultivation: fishing out all coilia ectenes parents cultured by environment regulation by using a net, exposing the coilia ectenes parents in the air for 15s, then putting the coilia ectenes parents in water for 285s, repeatedly fishing out and putting back for 10 times, adding a release agent into a culture pond after stress treatment, keeping the water temperature at 25 ℃ and the pH at 7.2, and feeding mysid and palaemon carinicauda until the coilia ectenes and the palaemon carinicaa are fed well, wherein the culture time is 7 days. The relieving agent is disodium dihydrogen pyrophosphate and 1-mannose nojirimycin hydrochloride, the usage amount of the disodium dihydrogen pyrophosphate is 8mM, and the usage amount of the 1-mannose nojirimycin hydrochloride is 90 mM.
The concentration of dissolved oxygen is controlled to be 8.5mg/L, the concentration of ammoniacal nitrogen is controlled to be less than 0.01mg/L, and the concentration of nitrite is controlled to be less than 0.01mg/L in the cultivation process.
The breeding process comprises feeding 2 times a day, 7 points in the morning and 5 points in the evening.
Soil is sucked once a day in the cultivation process.
Comparative example 1:
this comparative example is different from example 3 only in that the regulator used in the environmental control cultivation does not contain glyceryl monocaprylate.
Comparative example 2:
this comparative example is compared to example 3, except that the conditioner used in the environmental control incubation did not contain glycine ester hydrochloride.
Comparative example 3:
this comparative example is compared to example 6, except that the mitigating agent used in the intensive culture did not contain 1-mannose nojirimycin hydrochloride.
Comparative example 4:
this comparative example is compared to example 6, except that the mitigating agent used in the enhanced incubation did not contain disodium dihydrogen pyrophosphate.
Test example 1:
1. infrared detection
Test samples: glycine ester hydrochloride prepared in example 1.
The test method comprises the following steps: and performing infrared detection after tabletting by a KBr tabletting method. Scanning range: 500-4000cm -1 。
The infrared spectrum of glycine ester hydrochloride is shown in figure 1, 3536cm -1 Is shown as an amino nitrogen hydrogen absorption peak at 2480-2600cm -1 The characteristic absorption peak of the salt appears in the range of 1764cm -1 Is the infrared absorption peak of carbon-oxygen double bond in ester bond, 1108cm -1 The infrared absorption peak of the carbon-oxygen-carbon in the ester bond indicates that glycine ester hydrochloride is successfully obtained.
Test example 2:
1. coilia ectenes parent survival rate
Testing a sample: coilia ectenes parents bred by the methods of examples 1-6 and comparative examples 1-4. A control group was set, which differed from example 3 only in that no regulator was used in the environmental control incubation.
The result of the parent survival rate of the coilia ectenes is shown in fig. 2, wherein the parent survival rate of the coilia ectenes in the control group is 81%, and compared with the control group, the result in example 3 shows that the parent survival rate of the coilia ectenes is improved after the regulator is used in the environment-controlled cultivation; comparative example 1 compared to the control group, it is shown that the use of glycine ester hydrochloride does not improve the survival rate of coilia ectenes parents; comparative example 2 compared to the control group, it was shown that the use of glyceryl monocaprylate did not improve the survival rate of coilia ectenes parents; example 3 compared with comparative examples 1-2, the survival rate of coilia ectenes parents can be improved when the glycine ester hydrochloride and the glyceryl monocaprylate are used together, and the survival rate of coilia ectenes parents cannot be improved when the glycine ester hydrochloride or the glyceryl monocaprylate are used alone; examples 4-6 compared to example 3, show that the use of disodium dihydrogen pyrophosphate and 1-mannose nojirimycin hydrochloride increases the survival rate of coilia ectenes parents; example 6 shows that the use of disodium dihydrogen pyrophosphate has an effect of improving the survival rate of parent coilia ectenes, but the improvement effect is limited, compared with comparative example 3; example 6 compared to comparative example 4, it is shown that the use of 1-mannose nojirimycin hydrochloride does not increase the survival rate of coilia ectenes parents; example 6 compared to comparative examples 3-4, it is shown that disodium dihydrogen pyrophosphate and 1-mannose nojirimycin hydrochloride have the best effect when used together.
The survival rate of the coilia ectenes parents cultured by the method is high, and the survival rate of the coilia ectenes parents cultured by the method is more than 85 percent.
The post-spawning survival rate of the coilia nasus parent cultured by the method of example 1 is 90.4%, the post-spawning survival rate of the coilia nasus parent cultured by the method of example 2 is 91.0%, the post-spawning survival rate of the coilia nasus parent cultured by the method of example 3 is 91.7%, the post-spawning survival rate of the coilia nasus parent cultured by the method of example 4 is 92.9%, the post-spawning survival rate of the coilia nasus parent cultured by the method of example 5 is 93.2%, and the post-spawning survival rate of the coilia nasus parent cultured by the method of example 6 is 93.6%.
The survival rate of the coilia ectenes parents cultured by the method after spawning is more than 90 percent.
2. Coilia ectenes parent physical and chemical indexes
Test samples: coilia ectenes parents bred by the methods of examples 1-6 and comparative examples 1-4. A control group was set, which differed from example 3 only in that no regulator was used in the environmental control incubation. The blank group is normal cultivation, and does not include reinforced cultivation of environment-controlled cultivation.
The test method comprises the following steps: the fish is quickly taken out and immediately put into MS-222 with the mass concentration of 100mg/L for quick deep anesthesia, and the weight of the viscera and the weight of the liver are respectively weighed.
The visceral volume ratio and the rate of reduction of the visceral volume ratio are calculated according to the following formulas:
the visceral body ratio is visceral mass/body mass × 100%.
The reduction in the visceral volume ratio (ratio of blank group visceral volume to sample visceral volume ratio)/ratio of blank group visceral volume × 100%.
The liver body ratio and the liver body ratio decrease rate are calculated according to the following formulas:
liver-body ratio is liver mass/body mass x 100%.
The reduction rate of the liver body ratio is (blank liver body ratio-sample liver body ratio)/blank liver body ratio x 100%.
The parent-to-body dirty ratio of the blank group of coilia ectenes is 5.86%, the test results of the parent-to-body dirty ratio reduction rate of other test groups of coilia ectenes are shown in fig. 3, the parent-to-body dirty ratio reduction rate of the coilia ectenes of the control group is 23.82%, and compared with the control group, the test results of the example 3 show that the parent-to-body dirty ratio reduction rate of the coilia ectenes becomes small after the regulator is used in the environment regulation and cultivation; compared with a control group, the comparative example 1 shows that the effect of the use of the glycine ester hydrochloride on reducing the reduction rate of the parental visceral ratio of the coilia ectenes is not obvious; compared with the control group, the comparative example 2 shows that the effect of using the glyceryl monocaprylate on reducing the reduction rate of the parent-to-body ratio of the coilia ectenes is not obvious; example 3 in comparison with comparative examples 1 to 2, the decrease in the parent to body ratio of coilia ectenes could be reduced when the glycine ester hydrochloride and the glyceryl monocaprylate were used together, and the decrease in the parent to body ratio of coilia ectenes could not be reduced when the glycine ester hydrochloride or the glyceryl monocaprylate were used alone.
The parent liver-body ratio of the coilia ectenes in the blank group is 0.88%, the reduction rate test results of the parent liver-body ratio of the coilia ectenes in other test groups are shown in figure 4, the reduction rate of the parent liver-body ratio of the coilia ectenes in the control group is 31.53%, and compared with the control group, the reduction rate of the parent liver-body ratio of the coilia ectenes in the embodiment 3 is smaller after the regulator is used in environment regulation and cultivation; compared with a control group, the comparative example 1 shows that the effect of using the glycine ester hydrochloride on reducing the reduction rate of the liver-to-body ratio of the coilia ectenes parent is not obvious; compared with the control group, the comparative example 2 shows that the effect of using the glyceryl monocaprylate on reducing the reduction rate of the liver-to-body ratio of the coilia ectenes parents is not obvious; example 3 in comparison with comparative examples 1 to 2, the decrease rate of the liver ratio of the parent of the coilia ectenes was reduced when the glycine ester hydrochloride and the glyceryl monocaprylate were used together, and the decrease rate of the liver ratio of the parent of the coilia ectenes was not reduced when the glycine ester hydrochloride or the glyceryl monocaprylate was used alone.
The reduction rate of the dirty body ratio of the coilia ectenes parents is small after the culture, and the reduction rate of the dirty body ratio of the coilia ectenes parents is below 20 percent; the reduction rate of the liver body ratio of the coilia ectenes parents is small, and the reduction rate of the liver body ratio of the coilia ectenes parents is below 28%.
MDA detection
Test samples: the livers of the coilia ectenes parents cultured by the methods of examples 1-6 and comparative examples 1-4. A control group was set, which differed from example 3 only in that no regulator was used in the environmental control incubation.
The test method comprises the following steps: instructions for MDA test kit procedures were performed to detect MDA in liver.
The MDA content is (assay tube absorbance-assay blank tube absorbance)/(standard tube absorbance-standard blank tube absorbance) × standard substance concentration (10 nmol/mL)/sample protein concentration to be measured (mgprot/mL).
The detection result of the content of the MDA is shown in fig. 5, wherein the content of the MDA in the coilia ectenes parent of the control group is 71.6nmol/mL, and compared with the control group, the embodiment 3 shows that the content of the MDA in the coilia ectenes parent is reduced after the regulator is used in the environmental regulation and cultivation, namely the oxidative stress degree of the coilia ectenes parent is reduced; compared with the control group, the comparative example 1 shows that the content of MDA in the coilia ectenes parents cannot be basically reduced by using the glycine ester hydrochloride, namely the degree of oxidative stress reaction of the coilia ectenes parents cannot be reduced; compared with the control group, the comparative example 2 shows that the content of MDA in the coilia ectenes parents cannot be reduced basically by using the glyceryl monocaprylate, namely the oxidative stress degree of the coilia ectenes parents cannot be reduced; example 3 in comparison with comparative examples 1-2, the content of MDA in coilia nasus parents can be reduced, i.e., the degree of oxidative stress of coilia nasus parents is reduced, when the glycine ester hydrochloride and the glyceryl monocaprylate are used together, the content of MDA in coilia nasus parents cannot be reduced, i.e., the degree of oxidative stress of coilia nasus parents cannot be reduced, when the glycine ester hydrochloride or the glyceryl monocaprylate are used alone; examples 4-6 compared to example 3, show that the use of disodium dihydrogen pyrophosphate and 1-mannose nojirimycin hydrochloride reduces the amount of MDA in the coilia ectenes parents, i.e. reduces the degree of oxidative stress of the coilia ectenes parents; example 6 compared to comparative example 3, shows that the use of disodium dihydrogen pyrophosphate does not substantially reduce the amount of MDA in the parent of coilia ectenes; example 6 in comparison to comparative example 4, it is shown that the use of 1-mannose nojirimycin hydrochloride does not substantially reduce the amount of MDA in a parent of coilia ectenes; example 6 shows that disodium dihydrogen pyrophosphate and 1-mannose nojirimycin hydrochloride together have the best effect compared to comparative examples 3-4.
The content of MDA in the liver of the cultured coilia ectenes parent is low, and the content of MDA in the liver of the coilia ectenes parent is less than 65 nmol/mL.
The above embodiments are merely illustrative, and not restrictive, of the present invention, and those skilled in the art can make various changes and modifications without departing from the spirit and scope of the present invention. Therefore, all equivalent technical solutions also belong to the scope of the present invention, and the protection scope of the present invention should be defined by the claims.
Claims (6)
1. A method for improving survival rate of coilia ectenes parents comprises the following steps:
and (3) environment regulation and cultivation: firstly, carrying out starvation cultivation on the coilia ectenes parents, and then carrying out adjustment cultivation; the conditioning culture uses a conditioning agent containing glycine ester hydrochloride and glycerol monocaprylate, wherein the chemical formula of the glycine ester hydrochloride is HCl & NH 2 CH 2 COOCH(CH 3 )CH 2 CH(OCH 3 )OCH 3 。
2. The method for improving survival rate of coilia ectenes parents according to claim 1, wherein the method comprises the following steps: the using amount of the regulator glyceryl monocaprylate is 20-100 mM.
3. The method for improving survival rate of coilia ectenes parents according to claim 1, wherein the method comprises the following steps: the feeding amount in the adjusting cultivation is 5-60% of the full feeding amount.
4. The method for improving survival rate of coilia ectenes parents according to claim 1, wherein the method comprises the following steps: and the pH value of the water body in the environment regulation and cultivation is regulated to 6.5-8.
5. The method for improving survival rate of coilia ectenes parents according to claim 1, wherein the method comprises the following steps: the usage amount of glycine ester hydrochloride in the regulator is 10-100 mu M.
6. The method for improving survival rate of coilia ectenes parents according to claim 1, wherein the method comprises the following steps: the environment regulation breeding process comprises a parent breeding process.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110370869.5A CN113100125B (en) | 2021-04-07 | 2021-04-07 | Method for improving survival rate of coilia ectenes parents |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110370869.5A CN113100125B (en) | 2021-04-07 | 2021-04-07 | Method for improving survival rate of coilia ectenes parents |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN113100125A CN113100125A (en) | 2021-07-13 |
| CN113100125B true CN113100125B (en) | 2022-08-30 |
Family
ID=76715352
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202110370869.5A Active CN113100125B (en) | 2021-04-07 | 2021-04-07 | Method for improving survival rate of coilia ectenes parents |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN113100125B (en) |
Citations (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE2500840A1 (en) * | 1974-01-22 | 1975-07-24 | Lilly Co Eli | PROCESS FOR THE PREPARATION OF 1H-TETRAZOLE-1-ACETIC ACID |
| CN101891638A (en) * | 2009-05-19 | 2010-11-24 | 李其奎 | Method for producing glycine and glycine ester hydrochloride |
| CN101891637A (en) * | 2009-05-19 | 2010-11-24 | 李其奎 | Method for synthesizing glycine ester hydrochloride and processing mother liquid thereof |
| CN101906052A (en) * | 2009-06-05 | 2010-12-08 | 上海宝钢化工有限公司 | Method for preparing D-m-hydroxyphenylglycine |
| CN103193669A (en) * | 2013-02-27 | 2013-07-10 | 南京医科大学 | nNOS-Capon uncoupling compound, preparation method and application thereof |
| CN103288764A (en) * | 2013-04-25 | 2013-09-11 | 烟台绿叶动物保健品有限公司 | Quinhydroxy ketone glycine ester hydrochloride and preparation method thereof |
| CN104829478A (en) * | 2015-05-15 | 2015-08-12 | 河北科技大学 | Preparation process of D-phenylglycine methyl ester hydrochloride crystals |
| CN105237448A (en) * | 2015-11-26 | 2016-01-13 | 浙江拓普药业股份有限公司 | Method for synthesizing thiamphenicol glycine ester hydrochloride |
| CN106957236A (en) * | 2017-03-22 | 2017-07-18 | 浙江昂利康制药股份有限公司 | A kind of preparation method of Phenylglycine methyl ester methyl-hydrogen-sulfate monomethyl-sulfate salt |
| CN110128285A (en) * | 2019-06-24 | 2019-08-16 | 华北制药股份有限公司 | D-PG methyl ester hydrochloride/D- dihydro phenyl glycine methyl ester hydrochloride preparation method |
-
2021
- 2021-04-07 CN CN202110370869.5A patent/CN113100125B/en active Active
Patent Citations (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE2500840A1 (en) * | 1974-01-22 | 1975-07-24 | Lilly Co Eli | PROCESS FOR THE PREPARATION OF 1H-TETRAZOLE-1-ACETIC ACID |
| CN101891638A (en) * | 2009-05-19 | 2010-11-24 | 李其奎 | Method for producing glycine and glycine ester hydrochloride |
| CN101891637A (en) * | 2009-05-19 | 2010-11-24 | 李其奎 | Method for synthesizing glycine ester hydrochloride and processing mother liquid thereof |
| CN101906052A (en) * | 2009-06-05 | 2010-12-08 | 上海宝钢化工有限公司 | Method for preparing D-m-hydroxyphenylglycine |
| CN103193669A (en) * | 2013-02-27 | 2013-07-10 | 南京医科大学 | nNOS-Capon uncoupling compound, preparation method and application thereof |
| CN103288764A (en) * | 2013-04-25 | 2013-09-11 | 烟台绿叶动物保健品有限公司 | Quinhydroxy ketone glycine ester hydrochloride and preparation method thereof |
| CN104829478A (en) * | 2015-05-15 | 2015-08-12 | 河北科技大学 | Preparation process of D-phenylglycine methyl ester hydrochloride crystals |
| CN105237448A (en) * | 2015-11-26 | 2016-01-13 | 浙江拓普药业股份有限公司 | Method for synthesizing thiamphenicol glycine ester hydrochloride |
| CN106957236A (en) * | 2017-03-22 | 2017-07-18 | 浙江昂利康制药股份有限公司 | A kind of preparation method of Phenylglycine methyl ester methyl-hydrogen-sulfate monomethyl-sulfate salt |
| CN110128285A (en) * | 2019-06-24 | 2019-08-16 | 华北制药股份有限公司 | D-PG methyl ester hydrochloride/D- dihydro phenyl glycine methyl ester hydrochloride preparation method |
Also Published As
| Publication number | Publication date |
|---|---|
| CN113100125A (en) | 2021-07-13 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN112075551A (en) | Additive for improving survival rate of aquaculture animals and application thereof | |
| CN107182853A (en) | A kind of disease-resistant rapid-result perch cultural method and its application | |
| CN113099989A (en) | Rice and shrimp joint cropping ecological breeding method | |
| JPH02295422A (en) | Odor-improving method for scaleless fish with offensive odor | |
| CN113711953B (en) | Propagation and seedling raising method for hippocampus blossoming | |
| CN110742211A (en) | Grouper feed accelerant and using method thereof | |
| CN113100125B (en) | Method for improving survival rate of coilia ectenes parents | |
| WO2012079425A1 (en) | Biological control method for controlling prawn disease by clarias leather | |
| JP2012135285A (en) | Method for producing artificial cultivating water for cultivated fish | |
| CN106973834A (en) | A kind of Litopenaeus vannamei Broodstock feeding method | |
| Kestemont et al. | Larval rearing of the gudgeon, Gobio gobio L., under optimal conditions of feeding with the rotifer, Brachionus plicatilis OF Müller | |
| Kogane et al. | Improvement of larval rearing technique for mass seed production of snow crab Chionoecetes opilio | |
| Gupta | Reviews on the biology and culture of Silver Pomfret, Pampus argenteus (Euphrasen, 1788) | |
| Maslova | Problems and achievements in seed production of the Black Sea turbot in Russia | |
| CN116158499A (en) | High-salinity cultivation method for indoor workshop of prawn | |
| Rizal et al. | Effect of Additional Soybean Oil in Feed Commercial on Pangasius djambal Bleeker, 1846 for Growth, Feed Efficiency, and Survival Rate | |
| CN113475433B (en) | Method for cultivating channel catfish pseudo-female fish | |
| CN113598095B (en) | Rapid breeding technology for snout bream with transverse bands | |
| KR101323957B1 (en) | Novel Chlorella vulgaris capable low temperature growth and use thereof | |
| ŞAHİN | Larval rearing of the Black Sea turbot, Scophthalmus maximus (Linnaeus, 1758), under laboratory conditions | |
| Hesni et al. | Effects of temperature and salinity on survival and moulting of the narrow-clawed crayfish, Astacus leptodactylus Eschscholtz, 1823 (Decapoda, Astacidea). | |
| CN111374077A (en) | Penaeus vannamei farming method | |
| CN111374076A (en) | Penaeus vannamei feeding method | |
| CN114271225A (en) | Method for effectively preventing and treating white spot syndrome in salt pan shrimp culture | |
| van Maaren et al. | Temperature tolerance and oxygen consumption rates for juvenile southern flounder Paralichthys lethostigma acclimated to five different temperatures |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |