CN113092791A - Device and method for rapidly detecting female hormone and progestogen of human body - Google Patents
Device and method for rapidly detecting female hormone and progestogen of human body Download PDFInfo
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- CN113092791A CN113092791A CN202110414904.9A CN202110414904A CN113092791A CN 113092791 A CN113092791 A CN 113092791A CN 202110414904 A CN202110414904 A CN 202110414904A CN 113092791 A CN113092791 A CN 113092791A
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- 239000000583 progesterone congener Substances 0.000 title claims abstract description 86
- 229940088597 hormone Drugs 0.000 title claims description 14
- 239000005556 hormone Substances 0.000 title claims description 14
- 238000000034 method Methods 0.000 title claims description 9
- 239000000262 estrogen Substances 0.000 claims abstract description 80
- 229940011871 estrogen Drugs 0.000 claims abstract description 80
- 239000008280 blood Substances 0.000 claims abstract description 79
- 210000004369 blood Anatomy 0.000 claims abstract description 79
- 238000001514 detection method Methods 0.000 claims abstract description 49
- 238000001914 filtration Methods 0.000 claims abstract description 39
- 239000012528 membrane Substances 0.000 claims abstract description 28
- 229920000642 polymer Polymers 0.000 claims abstract description 27
- 238000002156 mixing Methods 0.000 claims abstract description 26
- 239000007788 liquid Substances 0.000 claims description 76
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 64
- 239000008213 purified water Substances 0.000 claims description 39
- 238000003756 stirring Methods 0.000 claims description 28
- 238000003860 storage Methods 0.000 claims description 26
- 238000005086 pumping Methods 0.000 claims description 25
- 239000003638 chemical reducing agent Substances 0.000 claims description 19
- 238000002347 injection Methods 0.000 claims description 18
- 239000007924 injection Substances 0.000 claims description 18
- RJKFOVLPORLFTN-LEKSSAKUSA-N Progesterone Chemical class C1CC2=CC(=O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H](C(=O)C)[C@@]1(C)CC2 RJKFOVLPORLFTN-LEKSSAKUSA-N 0.000 claims description 15
- 238000009826 distribution Methods 0.000 claims description 13
- 238000005070 sampling Methods 0.000 claims description 10
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- 102000004169 proteins and genes Human genes 0.000 claims description 8
- 108090000623 proteins and genes Proteins 0.000 claims description 8
- 238000000926 separation method Methods 0.000 claims description 8
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- 102000006771 Gonadotropins Human genes 0.000 description 1
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- 241000282412 Homo Species 0.000 description 1
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Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/74—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving hormones or other non-cytokine intercellular protein regulatory factors such as growth factors, including receptors to hormones and growth factors
- G01N33/76—Human chorionic gonadotropin including luteinising hormone, follicle stimulating hormone, thyroid stimulating hormone or their receptors
Abstract
The invention discloses a device for rapidly detecting human estrogen and progestogen, which comprises a blood mixing device, a blood filtering device and a estrogen and progestogen detecting device which are sequentially connected from top to bottom, wherein the blood mixing device comprises a tank body, a power unit arranged on the tank body and a stop valve fixedly arranged at the bottom of the tank body; blood filter equipment includes conical cover and lower conical cover, and lower conical cover sets firmly in the bottom of last conical cover, goes up and sets firmly polymer filtration membrane in the conical cover, goes up the last port and the stop valve connection of conical cover. The invention has the beneficial effects that: compact structure, improved detection precision of estrogen and progestogen, improved detection efficiency of estrogen and progestogen, and simple operation.
Description
Technical Field
The invention relates to the technical field of detection of estrogen and progestogen, in particular to a device and a method for quickly detecting estrogen and progestogen of a human body.
Background
The sex hormone includes female hormone and progestational hormone, etc., the sex hormone level plays an important role in human health, especially has an important role in female reproductive health, and many times, the change of the sex hormone causes the change of the female body to cause various diseases, such as adolescent dysfunctional uterine bleeding, polycystic ovary syndrome, amenorrhea and lactation syndrome, breast cancer, uterine cancer, etc., so the sex hormone detection is very important for human body. Scientific research shows that estrogen receptors are widely distributed on cell membranes of tissues and organs of a female whole body, and when estrogen is reduced, the tissues and the organs are degenerated or metabolized, so that a series of neurological, endocrine, metabolic and psychological imbalances occur, and syndrome groups with various systemic changes are caused. At present, ovarian function decline, estrogen reduction, gonadotropin increase and endocrine balance change are considered to be main causes of menopausal syndrome, and the level of estrogen reduction is found to be basically positively correlated with the severity of the menopausal syndrome.
In hospitals, testers detect estrogen and progestin from humans using electrochemical biosensors in which estrogen and progestin antibodies are embedded in carbon nanotubes as the main detection elements. The operation method comprises the steps of firstly extracting blood of a person to be detected, discharging the blood into a vessel, then adding a certain amount of purified water into the blood, mixing the purified water with the blood after adding the purified water so as to separate out the estrogen and the progestogen, extending a detection element into the vessel after mixing, and transmitting the collected content data of the estrogen and the progestogen to a computer by the detection element, thereby realizing the detection of the estrogen and the progestogen. However, although this detection method can detect estrogen and progestin, it has the following drawbacks: 1. after the blood is mixed with the purified water, the stirring direction of the conventional stirring device is single, so that the blood and the purified water can be sufficiently mixed by stirring and mixing for a long time, thereby reducing the detection efficiency. 2. Blood contains other macromolecular proteins, and the macromolecular proteins influence the detection precision of the detection element. 3. After the estrogen and the progestogen are diluted, the estrogen and the progestogen are reduced in unit volume, so that the estrogen and the progestogen are difficult to detect by the detecting element, thereby further reducing the detecting efficiency of the estrogen and the progestogen. Therefore, a detection device for improving the detection precision of the estrogen and the progestogen and improving the detection efficiency of the estrogen and the progestogen is needed.
Disclosure of Invention
The invention aims to overcome the defects of the prior art and provide a device and a method for quickly detecting the estrogen and the progestogen of a human body, which have the advantages of compact structure, improved accuracy and efficiency of detecting the estrogen and the progestogen and simple operation.
The purpose of the invention is realized by the following technical scheme: a device for rapidly detecting human estrogen and progestogen comprises a blood mixing device, a blood filtering device and a estrogen and progestogen detecting device which are sequentially connected from top to bottom, wherein the blood mixing device comprises a tank body, a power unit arranged on the tank body and a stop valve fixedly arranged at the bottom of the tank body, an ultrasonic generator is arranged on the outer wall of the tank body, a blood injection device and a purified water injection device are arranged at the top of the tank body, blades extending into the tank body are arranged on an output shaft of the power unit, an annular tube is connected between the blood injection device and the purified water injection device, and a plurality of liquid outlet holes are formed in the bottom surface of the annular tube;
the blood filtering device comprises an upper conical cover and a lower conical cover, the lower conical cover is fixedly arranged at the bottom of the upper conical cover, a sealed cavity is formed between the lower conical cover and the upper conical cover, a polymer filtering membrane is fixedly arranged in the upper conical cover, and the upper port of the upper conical cover is connected with a stop valve;
the female hormone and progestogen detection device comprises a temporary storage tank, a computer and a suspension liquid-pumping separation device arranged on the temporary storage tank, the temporary storage tank is communicated with the lower port of the lower conical cover, a valve is arranged on the side wall of the temporary storage tank, a sampling pipe is connected onto the valve, a detection element is arranged in the sampling pipe, and the detection element is electrically connected with the computer.
The power unit comprises a speed reducer and a motor, the speed reducer is fixedly arranged at the top of the tank body, the motor is fixedly arranged at the top of the speed reducer, an output shaft of the motor is connected with an input shaft of the speed reducer, a stirring shaft is connected to the output shaft of the speed reducer through a coupler, the stirring shaft is arranged in the tank body, and the blades are arranged on the stirring shaft.
The blood injection device comprises a liquid inlet pipe I and a funnel I, wherein the liquid inlet pipe I is fixedly arranged at the top of the tank body, the lower port of the liquid inlet pipe I is fixedly arranged on the annular pipe and communicated with the annular pipe, the upper port of the liquid inlet pipe I extends to the outside of the tank body, and the extension end of the upper port is connected with the funnel I.
The top of funnel I seals, and the top of funnel I sets firmly the graduated flask of intercommunication its inner chamber, and the bottom of graduated flask is connected with the choke valve.
The pure water injection device includes feed liquor pipe II and funnel II, and feed liquor pipe II sets firmly in jar body top, and feed liquor pipe II's lower port sets firmly on the ring pipe and communicates with the ring pipe, and feed liquor pipe II's last port extends in a jar external portion, and extends to serve and be connected with funnel II.
The top of funnel II is sealed, and funnel II's top is provided with the pure water inlet of intercommunication its inner chamber.
The upper conical cover is internally welded with a flow distribution plate, the flow distribution plate is arranged above the polymer filtering membrane, and a plurality of small holes are uniformly distributed on the flow distribution plate.
The outer wall of the upper conical cover is provided with external threads, the inner wall of the upper port of the lower conical cover is provided with internal threads, the internal threads of the lower conical cover are in threaded connection with the external threads of the upper conical cover, and the polymer filtering membrane is pressed between the upper conical cover and the lower conical cover under the threaded connection force.
Suspension liquid-pumping separation device includes water pump and liquid suction pipe, and liquid suction pipe slidable mounting has set firmly the fixed plate at the top of the jar of keeping in on the liquid suction pipe, and the cover is equipped with the spring on the liquid suction pipe, and the one end of spring welds on the jar of keeping in, and the other end welds on the fixed plate, and the top mouth of liquid suction pipe and the liquid suction mouth of water pump are through hose connection.
The method for rapidly detecting the estrogen and the progestogen of the human body by the device comprises the following steps:
s1, mixing the blood with purified water, which comprises the following steps:
s11, adding the collected blood into a measuring cylinder in advance, judging whether the added amount of the blood reaches the amount required by detection according to the scale value on the measuring cylinder, opening a throttle valve by a detector after the scale value is set, and finally enabling the blood to flow downwards from a liquid outlet hole through the throttle valve, a funnel I, a liquid inlet pipe I and an inner cavity of an annular pipe in sequence; simultaneously, a certain amount of purified water is added into the funnel II through a purified water adding port, and the purified water flows downwards from the liquid outlet hole through the funnel II, the liquid inlet pipe II and the inner cavity of the annular pipe in sequence;
s12, turning on a motor, reducing the speed of the motor through a speed reducer, driving a stirring shaft to rotate, driving blades to rotate by the stirring shaft, stirring and mixing blood and purified water by the blades to separate out estrogen and progestogen in the blood, and turning on an ultrasonic generator to accelerate the separation of the estrogen and the progestogen;
s2, filtering the blood, opening a valve, allowing the mixed liquid in the tank to pass through a stop valve and small holes on a flow distribution plate under the self gravity and fall onto a polymer filtering membrane, intercepting the polymer protein in the blood by the polymer filtering membrane, allowing the mixed liquid with the estrogen and the progestogen to pass through the polymer filtering membrane, and then allowing the mixed liquid to enter a temporary storage tank for collection, thereby realizing the filtration of the blood;
s3, standing the mixed solution in the temporary storage tank for a period of time, wherein the estrogen and the progestogen are in the lower layer of the temporary storage tank, the supernatant is in the upper layer of the hormone layer, the fixed plate is pressed downwards at the moment so that the liquid extracting pipe extends into the supernatant layer, then the water pump is turned on, the supernatant layer is extracted by the water pump, and the water pump is turned off after a certain amount of liquid is extracted;
s4, detecting the estrogen and the progestogen, wherein an operator opens a valve, the estrogen and the progestogen in the lower layer enter a sampling tube through the valve, then the valve is closed, the detection element transmits the collected data of the content of the estrogen and the progestogen to a computer through a lead, and the content of the estrogen and the progestogen in the detected blood is visually seen from the computer, so that the detection of the estrogen and the progestogen is realized.
The invention has the following advantages:
1. the top of the tank body of the invention is provided with a blood injection device and a purified water injection device, an output shaft of a power unit is provided with blades extending into the tank body, an annular tube is connected between the blood injection device and the purified water injection device, and the bottom surface of the annular tube is provided with a plurality of liquid outlet holes; the blood flows out from the liquid outlet hole through the throttle valve, the funnel I, the liquid inlet pipe I and the inner cavity of the annular pipe in sequence; meanwhile, a certain amount of purified water is added into the funnel II through the purified water adding port, the purified water flows downwards from the liquid outlet hole through the funnel II, the liquid inlet pipe II and the inner cavity of the annular pipe in sequence, and the blood and the purified water are mixed in advance, so that the mixing time of the blood and the purified water is shortened, the time for uniformly mixing is shortened, and the detection efficiency of the estrogen and the progestogen is improved.
2. The blood mixing device comprises a tank body, a power unit arranged on the tank body and a stop valve fixedly arranged at the bottom of the tank body, wherein an ultrasonic generator is arranged on the outer wall of the tank body; the blade stirs and mixes the blood and the purified water to separate out the estrogen and the progestogen in the blood, and simultaneously, the ultrasonic generator is turned on to accelerate the separation of the estrogen and the progestogen; compared with the traditional stirring device, the ultrasonic wave is adopted to separate out the estrogen and the progestogen by the unidirectional stirring of the traditional stirring device, so that the time for uniformly mixing is further shortened, and the detection efficiency of the estrogen and the progestogen is further improved.
3. The lower conical cover is fixedly arranged at the bottom of the upper conical cover, a sealed cavity is formed between the lower conical cover and the upper conical cover, a polymer filtering membrane is fixedly arranged in the upper conical cover, and the upper port of the upper conical cover is connected with a stop valve; the mixed liquid in the tank body passes through the stop valve and the small holes on the flow distribution plate under the self gravity and falls onto the polymer filtering membrane, the polymer filtering membrane intercepts the polymer protein in the blood, and the mixed liquid with the estrogen and the progestogen passes through the polymer filtering membrane and then enters the temporary storage tank for collection, thereby realizing the filtration of the blood; therefore, the influence of the high molecular protein in the blood on the detection precision of the subsequent detection element is avoided, and the detection precision of the detection element for detecting the estrogen and the progestogen is greatly improved.
4. The suspension liquid pumping and separating device comprises a water pump and a liquid pumping pipe, wherein the liquid pumping pipe is slidably arranged at the top of a temporary storage tank, a fixed plate is fixedly arranged on the liquid pumping pipe, a spring is sleeved on the liquid pumping pipe, one end of the spring is welded on the temporary storage tank, the other end of the spring is welded on the fixed plate, and a top port of the liquid pumping pipe is connected with a liquid pumping port of the water pump through a hose; pressing the fixed plate downwards to enable the liquid pumping pipe to extend into the clear liquid layer, then turning on the water pump, pumping out the clear liquid layer by the water pump, and turning off the water pump after a certain amount of liquid is pumped out; therefore, the content of the estrogen and the progestogen in the unit volume is increased, thereby improving the capability of a subsequent detection element to quickly detect the estrogen and the progestogen.
Drawings
FIG. 1 is a schematic structural view of the present invention;
FIG. 2 is a front view of FIG. 1;
FIG. 3 is a schematic structural view of the blood mixing device;
FIG. 4 is a front view of FIG. 3;
FIG. 5 is a schematic structural view of the annular tube;
FIG. 6 is a schematic view of the structure of the blood filtering device;
FIG. 7 is a front view of FIG. 6;
FIG. 8 is a schematic diagram of the construction of a female hormone and progestogen detecting device;
FIG. 9 is an enlarged partial view of portion I of FIG. 8;
FIG. 10 is an enlarged partial view of section II of FIG. 8;
in the figure, 1-a blood mixing device, 2-a blood filtering device, 3-a female hormone and progestogen detecting device, 4-a tank body, 5-a stop valve, 6-an ultrasonic generator, 7-a blade, 8-a ring pipe, 9-a liquid outlet hole, 10-an upper conical cover, 11-a lower conical cover, 12-a high molecular filtering membrane, 13-a temporary storage tank, 14-a computer, 15-a valve, 16-a sampling pipe, 17-a detecting element, 18-a speed reducer, 19-a motor, 20-a stirring shaft, 21-a liquid inlet pipe I, 22-a funnel I, 23-a measuring cylinder, 24-a liquid inlet pipe II, 25-a funnel II, 26-a purified water inlet, 27-a flow dividing plate, 28-a small hole and 29-an external thread, 30-water pump, 31-liquid suction pipe, 32-fixed plate, 33-spring, 34-hose and 35-throttle valve.
Detailed Description
The invention will be further described with reference to the accompanying drawings, without limiting the scope of the invention to the following:
as shown in fig. 1 to 10, a device for rapidly detecting estrogen and progestin in a human body comprises a blood mixing device 1, a blood filtering device 2 and a estrogen and progestin detecting device 3 which are sequentially connected from top to bottom, wherein the blood mixing device 1 comprises a tank body 4, a power unit arranged on the tank body 4 and a stop valve 5 fixedly arranged at the bottom of the tank body 4, an ultrasonic generator 6 is arranged on the outer wall of the tank body 4, a blood injecting device and a purified water injecting device are arranged at the top of the tank body 4, a blade 7 extending into the tank body 4 is arranged on an output shaft of the power unit, an annular tube 8 is connected between the blood injecting device and the purified water injecting device, and a plurality of liquid outlet holes 9 are arranged on the bottom surface of the annular tube 8. Blood filter equipment 2 includes conical cover 10 and lower conical cover 11, and lower conical cover 11 sets firmly in the bottom of last conical cover 10, and forms airtight chamber between the conical cover 10, goes up and has set firmly polymer filtration membrane 12 in the conical cover 10, and the last port and the stop valve 5 of last conical cover 10 are connected. The female hormone and progestogen detection device 3 comprises a temporary storage tank 13, a computer 14 and a suspension liquid-extracting and separating device arranged on the temporary storage tank 13, the temporary storage tank 13 is communicated with the lower port of the lower conical cover 11, a valve 15 is arranged on the side wall of the temporary storage tank 13, a sampling pipe 16 is connected onto the valve 15, a detection element 17 is arranged in the sampling pipe 16, and the detection element 17 is electrically connected with the computer 14. The power unit comprises a speed reducer 18 and a motor 19, the speed reducer 18 is fixedly arranged at the top of the tank body 4, the motor 19 is fixedly arranged at the top of the speed reducer 18, an output shaft of the motor 19 is connected with an input shaft of the speed reducer 18, an output shaft of the speed reducer 18 is connected with a stirring shaft 20 through a coupler, the stirring shaft 20 is arranged in the tank body 4, and the blades 7 are arranged on the stirring shaft 20.
The blood injection device comprises a liquid inlet pipe I21 and a funnel I22, wherein the liquid inlet pipe I21 is fixedly arranged at the top of the tank body 4, the lower port of the liquid inlet pipe I21 is fixedly arranged on the annular pipe 8 and is communicated with the annular pipe 8, the upper port of the liquid inlet pipe I21 extends to the outside of the tank body 4, and the extending end of the liquid inlet pipe I21 is connected with the funnel I22; the top of the funnel I22 is closed, the top of the funnel I22 is fixedly provided with a measuring cylinder 23 communicated with the inner cavity of the funnel I, and the bottom of the measuring cylinder 23 is connected with a throttle valve 35.
The purified water injection device comprises a liquid inlet pipe II24 and a funnel II25, the liquid inlet pipe II24 is fixedly arranged at the top of the tank body 4, the lower port of the liquid inlet pipe II24 is fixedly arranged on the annular pipe 8 and is communicated with the annular pipe 8, the upper port of the liquid inlet pipe II24 extends to the outside of the tank body 4, and the extending end is connected with the funnel II 25; the top of the funnel II25 is closed, and the top of the funnel II25 is provided with a purified water adding port 26 communicated with the inner cavity of the funnel II 25.
Go up the interior welded flow distribution plate 27 of conical cover 10, flow distribution plate 27 sets up in the top of polymer filtration membrane 12, and evenly distributed has a plurality of apertures 28 on flow distribution plate 27, and aperture 28 makes the mixed liquid divide equally fall polymer filtration membrane 12 on to avoid polymer filtration membrane to mix the impact force of liquid and damage. The outer wall of the upper conical cover 10 is provided with an external thread 29, the inner wall of the upper port of the lower conical cover 11 is provided with an internal thread, the internal thread of the lower conical cover 11 is in threaded connection with the external thread 29 of the upper conical cover 10, and the polymer filtering membrane 12 is pressed between the upper conical cover 10 and the lower conical cover 11 under the threaded connection force. When the polymer filtering membrane 12 is to be replaced, the lower conical cover 11 can be screwed down from the upper conical cover 10, so that the polymer filtering membrane 12 can be replaced conveniently, and later maintenance operation is facilitated.
Suspension liquid-pumping separation device includes water pump 30 and liquid-pumping pipe 31, and liquid-pumping pipe 31 slidable mounting has set firmly fixed plate 32 at the top of jar 13 of keeping in on the liquid-pumping pipe 31, and the cover is equipped with spring 33 on the liquid-pumping pipe 31, and the one end of spring 33 welds on jar 13 of keeping in, and the other end welds on fixed plate 32, and the top port of liquid-pumping pipe 31 is connected through hose 34 with the liquid-pumping mouth of water pump 30.
The method for rapidly detecting the estrogen and the progestogen of the human body by the device comprises the following steps:
s1, mixing the blood with purified water, which comprises the following steps:
s11, adding the collected blood into the measuring cylinder 23 in advance, judging whether the added amount of the blood reaches the amount required by detection according to the scale value on the measuring cylinder 23, opening the throttle valve 35 by a detector after the set scale is reached, and finally enabling the blood to flow downwards from the liquid outlet 9 through the throttle valve 35, the funnel I22, the liquid inlet pipe I21 and the inner cavity of the annular pipe 8 in sequence; simultaneously, a certain amount of purified water is added into the funnel II25 through the purified water adding port 26, and the purified water flows downwards from the liquid outlet hole 9 through the funnel II25, the liquid inlet pipe II24 and the inner cavity of the annular pipe 8 in sequence; in the process, the blood and the purified water are mixed in advance, so that the mixing time of the blood and the purified water is shortened, the time for uniformly mixing is shortened, and the detection efficiency of the estrogen and the progestogen is improved;
s12, turning on a motor 19, reducing the speed of the torque of the motor 19 through a speed reducer 18, driving a stirring shaft 20 to rotate, driving a blade 7 to rotate by the stirring shaft 20, stirring and mixing blood and purified water by the blade 7 to separate out estrogen and progestogen in the blood, and turning on an ultrasonic generator 6 at the same time, wherein the ultrasonic generator 6 accelerates the separation of the estrogen and the progestogen; compared with the traditional stirring device, the single-direction stirring device has the advantages that ultrasonic waves are adopted to separate out the estrogen and the progestogen, so that the time for uniformly mixing is further shortened, and the detection efficiency of the estrogen and the progestogen is further improved;
s2, filtering the blood, opening a valve 15, allowing the mixed liquid in the tank 4 to pass through the stop valve 5 and the small hole 28 on the flow distribution plate 27 under the self gravity and fall onto the polymer filtering membrane 12, intercepting the polymer protein in the blood by the polymer filtering membrane 12, allowing the mixed liquid with the estrogen and the progestogen to pass through the polymer filtering membrane 12, and then entering the temporary storage tank 13 for collection, thereby realizing the filtration of the blood; therefore, the influence of high molecular protein in blood on the detection precision of a subsequent detection element is avoided, and the female hormone and progestogen detection precision of the detection element is greatly improved;
s3, standing the mixed solution in the temporary storage tank 13 for a period of time, wherein the estrogen and the progestogen are in the lower layer of the temporary storage tank 13, the supernatant is in the upper layer of the hormone layer, the fixed plate 32 is pressed downwards at the moment to enable the liquid extracting pipe 31 to extend into the supernatant layer, then the water pump 30 is started, the supernatant layer is extracted by the water pump 30, and the water pump 30 is closed after a certain amount of liquid is extracted; therefore, the content of the estrogen and the progestogen in a unit volume is increased, so that the condition that a subsequent detection element can quickly detect the estrogen and the progestogen is improved;
s4, detecting the estrogen and the progestogen, wherein an operator opens the valve 15, the estrogen and the progestogen in the lower layer enter the sampling pipe 16 through the valve 15, then the valve 15 is closed, the detection element 17 transmits the acquired data of the content of the estrogen and the progestogen to the computer 14 through a lead, and the content of the estrogen and the progestogen in the detected blood is visually observed from the computer 14, so that the detection of the estrogen and the progestogen is realized.
Finally, it should be noted that: although the present invention has been described in detail with reference to the foregoing embodiments, it will be apparent to those skilled in the art that changes may be made in the embodiments and/or equivalents thereof without departing from the spirit and scope of the invention. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.
Claims (10)
1. A device for rapidly detecting estrogen and progestin in a human, comprising: the device comprises a blood mixing device (1), a blood filtering device (2) and a female hormone and progestogen detection device (3) which are sequentially connected from top to bottom, wherein the blood mixing device (1) comprises a tank body (4), a power unit arranged on the tank body (4) and a stop valve (5) fixedly arranged at the bottom of the tank body (4), an ultrasonic generator (6) is arranged on the outer wall of the tank body (4), a blood injection device and a purified water injection device are arranged at the top of the tank body (4), a blade (7) extending into the tank body (4) is arranged on an output shaft of the power unit, an annular tube (8) is connected between the blood injection device and the purified water injection device, and a plurality of liquid outlet holes (9) are formed in the bottom surface of the annular tube (8);
the blood filtering device (2) comprises an upper conical cover (10) and a lower conical cover (11), the lower conical cover (11) is fixedly arranged at the bottom of the upper conical cover (10), a sealed cavity is formed between the lower conical cover and the upper conical cover (10), a polymer filtering membrane (12) is fixedly arranged in the upper conical cover (10), and the upper port of the upper conical cover (10) is connected with the stop valve (5);
the female hormone and progestogen detection device (3) comprises a temporary storage tank (13), a computer (14) and a suspension liquid pumping and separating device arranged on the temporary storage tank (13), the temporary storage tank (13) is communicated with a lower port of a lower conical cover (11), a valve (15) is arranged on the side wall of the temporary storage tank (13), a sampling pipe (16) is connected onto the valve (15), a detection element (17) is arranged in the sampling pipe (16), and the detection element (17) is electrically connected with the computer (14).
2. The device for rapidly detecting estrogen and progestin in human body according to claim 1, wherein: the power unit comprises a speed reducer (18) and a motor (19), the speed reducer (18) is fixedly arranged at the top of the tank body (4), the motor (19) is fixedly arranged at the top of the speed reducer (18), an output shaft of the motor (19) is connected with an input shaft of the speed reducer (18), a stirring shaft (20) is connected to an output shaft of the speed reducer (18) through a coupler, the stirring shaft (20) is arranged in the tank body (4), and the blades (7) are arranged on the stirring shaft (20).
3. The device for rapidly detecting estrogen and progestin in human body according to claim 1, wherein: the blood injection device comprises a liquid inlet pipe I (21) and a funnel I (22), wherein the liquid inlet pipe I (21) is fixedly arranged at the top of the tank body (4), the lower port of the liquid inlet pipe I (21) is fixedly arranged on the annular pipe (8) and communicated with the annular pipe (8), the upper port of the liquid inlet pipe I (21) extends to the outside of the tank body (4), and the extension end is connected with the funnel I (22).
4. The device for rapidly detecting estrogen and progestin in human body according to claim 3, wherein: the top of funnel I (22) is sealed, and the top of funnel I (22) sets firmly graduated flask (23) that communicate its inner chamber, and the bottom of graduated flask (23) is connected with choke valve (35).
5. The device for rapidly detecting estrogen and progestin in human body according to claim 1, wherein: the pure water injection device includes feed liquor pipe II (24) and funnel II (25), and feed liquor pipe II (24) set firmly in jar body (4) top, and the lower port of feed liquor pipe II (24) sets firmly on annular tube (8) and with annular tube (8) intercommunication, and the last port of feed liquor pipe II (24) extends in jar body (4) outside, and extends to serve and be connected with funnel II (25).
6. The device for rapidly detecting estrogen and progestin in human body according to claim 5, wherein: the top of funnel II (25) is sealed, and the top of funnel II (25) is provided with the pure water inlet (26) of its inner chamber of intercommunication.
7. The device for rapidly detecting estrogen and progestin in human body according to claim 1, wherein: go up conical cover (10) internal welding and have flow distribution plate (27), flow distribution plate (27) set up in polymer filter membrane's (12) top, and evenly distributed has a plurality of apertures (28) on flow distribution plate (27).
8. The device for rapidly detecting estrogen and progestin in human body according to claim 1, wherein: the outer wall of the upper conical cover (10) is provided with an external thread (29), the inner wall of the upper port of the lower conical cover (11) is provided with an internal thread, the internal thread of the lower conical cover (11) is in threaded connection with the external thread (29) of the upper conical cover (10), and the polymer filtering membrane (12) is pressed between the upper conical cover (10) and the lower conical cover (11) under the threaded connection force.
9. The device for rapidly detecting estrogen and progestin in human body according to claim 1, wherein: suspension liquid-pumping separation device includes water pump (30) and liquid-pumping pipe (31), and liquid-pumping pipe (31) slidable mounting has set firmly fixed plate (32) on liquid-pumping pipe (31), and the cover is equipped with spring (33) on liquid-pumping pipe (31), and the one end of spring (33) welds on temporary storage tank (13), and the other end welds on fixed plate (32), and the top port of liquid-pumping pipe (31) is connected through hose (34) with the drawing liquid mouth of water pump (30).
10. The method for rapidly detecting estrogen and progestin in human bodies according to any of claims 1-9, comprising the steps of: it comprises the following steps:
s1, mixing the blood with purified water, which comprises the following steps:
s11, adding the collected blood into a measuring cylinder (23) in advance, judging whether the added amount of the blood reaches the amount required by detection according to the scale value on the measuring cylinder (23), opening a throttle valve (35) by a detector when the set scale is reached, and finally enabling the blood to flow out downwards from a liquid outlet hole (9) through the inner cavities of the throttle valve (35), a funnel I (22), a liquid inlet pipe I (21) and a ring pipe (8) in sequence; meanwhile, a certain amount of purified water is added into the funnel II (25) through a purified water adding port (26), and the purified water finally flows downwards from the liquid outlet hole (9) through the inner cavities of the funnel II (25), the liquid inlet pipe II (24) and the annular pipe (8) in sequence;
s12, turning on a motor (19), reducing the speed of the torque of the motor (19) through a speed reducer (18) and then driving a stirring shaft (20) to rotate, driving a blade (7) to rotate through the stirring shaft (20), stirring and mixing blood and purified water through the blade (7) to separate out estrogen and progestogen in the blood, and turning on an ultrasonic generator (6) at the same time to accelerate the separation of the estrogen and the progestogen through the ultrasonic generator (6);
s2, filtering the blood, opening a valve (15), allowing the mixed liquid in the tank body (4) to pass through a stop valve (5) and a small hole (28) on a flow distribution plate (27) under the self gravity and fall onto a high molecular filter membrane (12), intercepting high molecular proteins in the blood by the high molecular filter membrane (12), allowing the mixed liquid with the estrogen and the progestogen to pass through the high molecular filter membrane (12), and then entering a temporary storage tank (13) for collection, thereby realizing the filtration of the blood;
s3, standing the mixed solution in the temporary storage tank (13) for a period of time, enabling the estrogen and the progestogen to be positioned at the lower layer of the temporary storage tank (13), enabling the supernatant to be positioned at the upper layer of the hormone layer, pressing the fixing plate (32) downwards to enable the liquid extracting pipe (31) to extend into the supernatant layer, then turning on the water pump (30), enabling the water pump (30) to extract the supernatant layer, and turning off the water pump (30) after extracting a certain amount;
s4, detecting the estrogen and the progestogen, wherein an operator opens a valve (15), the estrogen and the progestogen in the lower layer enter a sampling pipe (16) through the valve (15), then the valve (15) is closed, at the moment, a detection element (17) transmits the data of the content of the estrogen and the progestogen collected by the detection element to a computer (14) through a lead, and the content of the estrogen and the progestogen in the detected blood is visually seen from the computer (14), so that the detection of the estrogen and the progestogen is realized.
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Address after: Room 101, Building 2, No. 3684 Tuanjie Road, Qingdao Area, China (Shandong) Pilot Free Trade Zone, Qingdao City, Shandong Province, 265503 Patentee after: Qingdao Funuo biomedical Co.,Ltd. Address before: 266101 1st floor, building 1, taikeda Industrial Park, 3 Zhuzhou Road, Laoshan District, Qingdao City, Shandong Province Patentee before: Qingdao Funuo biomedical Co.,Ltd. |