CN110514488A - A kind of sampler for biotechnology separation - Google Patents
A kind of sampler for biotechnology separation Download PDFInfo
- Publication number
- CN110514488A CN110514488A CN201810492674.6A CN201810492674A CN110514488A CN 110514488 A CN110514488 A CN 110514488A CN 201810492674 A CN201810492674 A CN 201810492674A CN 110514488 A CN110514488 A CN 110514488A
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- China
- Prior art keywords
- separator box
- sampler
- sample
- sampler barrel
- tube
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/286—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q involving mechanical work, e.g. chopping, disintegrating, compacting, homogenising
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- Physics & Mathematics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention discloses a kind of samplers for biotechnology separation, including separator box, sampler barrel and dosing tank, the separator box is between sampler barrel and dosing tank, rotating electric machine is equipped in separator box center of top, the output end of rotating electric machine is connected with the agitating shaft protruded into inside separator box, the agitating shaft is equipped with multiple rotary crushing blades, it is equipped with the fixation crushing knife being staggered in the vertical direction with rotary crushing blades on separation chamber interior wall, the sample inlet being connected with inside separator box is also provided at the top of separator box；The present apparatus crushes sample by the way that the rotary crushing blades being staggered and fixed crushing knife are arranged in separator box, and medical fluid is conveyed into sample layer by dosing tank, realize first crushing, the rear removing of sample, to ensure that separative efficiency, the accuracy of sampling is improved, and by the setting of sampler barrel, sampling process is convenient, a possibility that reducing maloperation, and the pollution of sample is avoided simultaneously.
Description
Technical field
The present invention relates to biotechnology, specifically a kind of sampler for biotechnology separation.
Background technique
Cell separation technology includes centrifugation technique, flow cytometry and cell electrophoresis.Centrifugation is research such as nucleus, line grain
Body, golgiosome, lysosome and microbody and various macromolecular basic means.Flow cytometry is carried out fastly to individual cells
A special kind of skill of fast quantitative analysis and sorting.Cell electrophoresis refers to that cell surface is with net positive or negative electricity under certain PH value
Under the action of extra electric field swimming can occur for lotus.
Structure is complicated for the separation sampler of current biotechnology, and price is high, only a small amount of for clinic, and is separating
It is complicated for operation in the process, it is also easy to be contaminated cell sample, influences to sample accuracy.
Summary of the invention
The purpose of the present invention is to provide a kind of samplers for biotechnology separation, to solve above-mentioned background technique
The problem of middle proposition.
To achieve the above object, the invention provides the following technical scheme:
A kind of sampler for biotechnology separation, including separator box, sampler barrel and dosing tank, the separator box, which is located at, to be taken
Between sample cylinder and dosing tank, it is equipped with rotating electric machine in separator box center of top, the output end of rotating electric machine, which is connected with, protrudes into separation
Agitating shaft inside case, the agitating shaft are equipped with multiple rotary crushing blades, are equipped with and rotary crushing blades on separation chamber interior wall
The fixation crushing knife being staggered in the vertical direction, is also provided with the sample being connected with inside separator box at the top of separator box
Entrance；It is equipped with sample layer in separation lower box part, sample layer and separator box interconnect and in the junction of sample layer and separator box
Equipped with separating plate, multiple plate holes are offered on the separating plate；The dosing lower box part passes through connecting tube and phase inside sample layer
Connection, lower part is equipped with horizontal filter membrane in dosing tank, and filter membrane is located at the lower section of connecting tube and dosing tank junction, is adding
It is equipped with spray tank above medicine-chest, is equipped with delivery pump in spray tank, the output end of delivery pump connects drug-feeding tube, under the drug-feeding tube
End is protruded into dosing tank and is connected with horizontal spray chamber, offers multiple spray orifices in spray chamber bottom, sets below spray chamber
There is web plate；The sampler barrel is cylindrical tube, and the piston being slidably connected with sampler barrel inner wall, the piston are equipped in sampler barrel
Top surface is equipped with push rod, and handle is stretched out above sampler barrel and be equipped in push rod upper end, is equipped with inlet tube and out liquid in sampler barrel bottom
Pipe, inlet tube lower end is protruded into sample layer.
As a preferred solution of the present invention: heat-exchange tube, the heat-exchange tube are equipped with before in the separator box wall box
In the shape of a spiral, it is connected separately with water inlet pipe and outlet pipe in heat-exchange tube upper and lower ends, water inlet pipe connects the supply of external heat-exchanging liquid
Equipment.
As further preferred embodiment of the invention: the rotary crushing blades are in arc-shaped with fixed crushing knife.
As further preferred embodiment of the invention: the sample layer section in the horizontal direction is greater than separator box in water
Square to section.
As further preferred embodiment of the invention: the plate hole aperture is 150 mesh.
As further preferred embodiment of the invention: the diameter of the handle is greater than the diameter of sampler barrel.
As further preferred embodiment of the invention: being equipped with check valve on the inlet tube and outlet tube.
Compared with prior art, the beneficial effects of the present invention are: the present apparatus is staggered by being arranged in separator box
Rotary crushing blades and fixed crushing knife crush sample, and medical fluid is conveyed into sample layer by dosing tank, realize sample
First crushing, rear removing, to ensure that separative efficiency, improve the accuracy of sampling, and by the setting of sampler barrel, take
A possibility that sample process is convenient, reduces maloperation, and the pollution of sample is avoided simultaneously.
Detailed description of the invention
Fig. 1 is the structural diagram of the present invention.
Fig. 2 is the internal structure cross-sectional view of sampler barrel in the present invention.
Fig. 3 is the top view of separating plate in the present invention.
1- separator box in figure, 2- sampler barrel, 3- dosing tank, 4- sample layer, 5- separating plate, 6- agitating shaft, 7- rotating shredder
Knife, the fixed crushing knife of 8-, 9- heat-exchange tube, 10- water inlet pipe, 11- outlet pipe, 12- connecting tube, 13- spray tank, 14- drug-feeding tube,
15- spray chamber, 16- web plate, 17- filter membrane, 18- sample inlet, 19- handle, 20- push rod, 21- piston, 22- inlet tube, 23-
Outlet tube, 24- plate hole.
Specific embodiment
Following will be combined with the drawings in the embodiments of the present invention, and technical solution in the embodiment of the present invention carries out clear, complete
Site preparation description, it is clear that described embodiments are only a part of the embodiments of the present invention, instead of all the embodiments.It is based on
Embodiment in the present invention, it is obtained by those of ordinary skill in the art without making creative efforts every other
Embodiment shall fall within the protection scope of the present invention.
Please refer to Fig. 1-3, in the embodiment of the present invention, a kind of sampler for biotechnology separation, including separator box
1, sampler barrel 2 and dosing tank 3, the separator box 1 are equipped between sampler barrel 2 and dosing tank 3 in 1 center of top of separator box
Rotating electric machine, the output end of rotating electric machine are connected with the agitating shaft 6 protruded into inside separator box 1, and the agitating shaft 6 is equipped with multiple
Rotary crushing blades 7 are equipped with the fixation crushing knife being staggered in the vertical direction with rotary crushing blades 7 on 1 inner wall of separator box
8, the rotary crushing blades 7 and fixed crushing knife 8 are in arc-shaped, are also provided at the top of separator box 1 and 1 inside phase of separator box
The sample inlet 18 of connection；It is equipped with sample layer 4 in 1 lower part of separator box, the section in the horizontal direction of sample layer 4 is greater than separator box 1
Section in the horizontal direction, sample layer 4 are interconnected with separator box 1 and are equipped in sample layer 4 with the junction of separator box 1 and separate
Plate 5, multiple plate holes 24 are offered on the separating plate 5, and 24 aperture of plate hole is 150 mesh；When being separated, by biological sample
Poured into separator box 1 by sample inlet 18, start rotating electric machine, by rotary crushing blades 7 and fixed crushing knife 8 to sample into
It is stood after row is broken, the sample of qualified size is entered in sample layer 4 by the plate hole 24 on separating plate 5 after being crushed；
In 1 wall box of separator box before be equipped with heat-exchange tube 9, the heat-exchange tube 9 in the shape of a spiral, on heat-exchange tube 9
Lower both ends are connected separately with water inlet pipe 10 and outlet pipe 11, and water inlet pipe 10 connects external heat-exchanging liquid supply arrangement；It is being separated
In the process, heat exchange liquid is sent into heat-exchange tube 9 by water inlet pipe 10, and then guarantees that sample remains permanent during the separation process
Temperature is determined, to avoid sample from losing activity under temperature difference environment, to guarantee the accuracy of sampling；
3 lower part of dosing tank inside connecting tube 12 and sample layer 4 by being connected, and lower part is equipped with horizontal in dosing tank 3
Filter membrane 17, filter membrane 17 are located at the lower section of connecting tube 12 Yu 3 junction of dosing tank, and spray tank 13 is equipped with above dosing tank 3,
Delivery pump is equipped in spray tank 13, the output end of delivery pump connects drug-feeding tube 14, and dosing tank 3 is protruded into 14 lower end of drug-feeding tube
It is interior and be connected with horizontal spray chamber 15, multiple spray orifices are offered in 15 bottom of spray chamber, for will enter in spray chamber 15
Medical fluid is sprayed, and web plate 16 is equipped with below spray chamber 15, the web plate is for guaranteeing the uniform of medicine liquid spray；After separation
Sample enter in sample layer 4 after, medical fluid is sprayed by dosing tank 3 and is sent into sample layer 4, is mixed by medical fluid with sample,
To make cell separate, to ensure that separative efficiency；
The sampler barrel 2 is cylindrical tube, and the piston 21 being slidably connected with 2 inner wall of sampler barrel, the work are equipped in sampler barrel 2
21 top surfaces are filled in equipped with push rod 20, and 20 upper end of push rod stretches out 2 top of sampler barrel and is equipped with handle 19, and the diameter of the handle 19 is big
In the diameter of sampler barrel 2, it is equipped with inlet tube 22 and outlet tube 23 in 2 bottom of sampler barrel, on inlet tube 22 and outlet tube 23
Equipped with check valve, 22 lower end of inlet tube is protruded into sample layer 4, and during being sampled, sample is mixed in sample layer 4 with medical fluid
After separation, handle 19 is pulled up, closed check valve on outlet tube 23, check valve is opened on inlet tube 22, sample in sample layer 4
Entered in sampler barrel 2 by inlet tube 22, when needing to take out sample from sampler barrel 2, pushes handle 19, inlet tube 22 downwards
Upper closed check valve, check valve is opened on outlet tube 23, and the sample in sampler barrel 2 is sent out by outlet tube 23.
The working principle of the invention is: the present apparatus in separator box 1 by being arranged the rotary crushing blades being staggered 7 and consolidating
Determine crushing knife 8 to crush sample, and convey medical fluid into sample layer 4 by dosing tank 3, realize sample first crushing, after
Removing improves the accuracy of sampling, and pass through the setting of sampler barrel 2, sampling process side to ensure that separative efficiency
Just, reduce a possibility that maloperation, and avoid the pollution of sample simultaneously.
It is obvious to a person skilled in the art that invention is not limited to the details of the above exemplary embodiments, Er Qie
In the case where without departing substantially from spirit or essential attributes of the invention, the present invention can be realized in other specific forms.Therefore, no matter
From the point of view of which point, the present embodiments are to be considered as illustrative and not restrictive, and the scope of the present invention is by appended power
Benefit requires rather than above description limits, it is intended that all by what is fallen within the meaning and scope of the equivalent elements of the claims
Variation is included within the present invention.Any reference signs in the claims should not be construed as limiting the involved claims.
In addition, it should be understood that although this specification is described in terms of embodiments, but not each embodiment is only wrapped
Containing an independent technical solution, this description of the specification is merely for the sake of clarity, and those skilled in the art should
It considers the specification as a whole, the technical solutions in the various embodiments may also be suitably combined, forms those skilled in the art
The other embodiments being understood that.
Claims (7)
1. a kind of sampler for biotechnology separation, including separator box, sampler barrel and dosing tank, which is characterized in that institute
Separator box is stated between sampler barrel and dosing tank, is equipped with rotating electric machine, the output end of rotating electric machine in separator box center of top
It is connected with the agitating shaft protruded into inside separator box, the agitating shaft is equipped with multiple rotary crushing blades, sets on separation chamber interior wall
Have the fixation crushing knife being staggered in the vertical direction with rotary crushing blades, be also provided at the top of separator box in separator box
The sample inlet that portion is connected；Be equipped with sample layer in separation lower box part, sample layer and separator box interconnect and sample layer with
The junction of separator box is equipped with separating plate, offers multiple plate holes on the separating plate；The dosing lower box part passes through connecting tube
It is connected with inside sample layer, lower part is equipped with horizontal filter membrane in dosing tank, and filter membrane is located at connecting tube and dosing tank connects
The lower section for meeting place is equipped with spray tank above dosing tank, and delivery pump is equipped in spray tank, and the output end of delivery pump is connected into medicine
Pipe, the drug-feeding tube lower end are protruded into dosing tank and are connected with horizontal spray chamber, offer multiple spray orifices in spray chamber bottom,
Web plate is equipped with below spray chamber；The sampler barrel is cylindrical tube, is equipped in sampler barrel and is slidably connected with sampler barrel inner wall
Piston, the piston-top surface be equipped with push rod, push rod upper end stretch out sampler barrel above simultaneously handle is installed, set in sampler barrel bottom
There are inlet tube and outlet tube, inlet tube lower end is protruded into sample layer.
2. a kind of sampler for biotechnology separation according to claim 1, which is characterized in that the separator box
In wall box before be equipped with heat-exchange tube, the heat-exchange tube in the shape of a spiral, heat-exchange tube upper and lower ends be connected separately with into
Water pipe and outlet pipe, water inlet pipe connect external heat-exchanging liquid supply arrangement.
3. a kind of sampler for biotechnology separation according to claim 1, which is characterized in that the rotation powder
Broken knife and fixed crushing knife are in arc-shaped.
4. a kind of sampler for biotechnology separation according to claim 1, which is characterized in that the sample layer
Section in the horizontal direction is greater than separator box section in the horizontal direction.
5. a kind of sampler for biotechnology separation according to claim 1, which is characterized in that the plate hole hole
Diameter is 150 mesh.
6. a kind of sampler for biotechnology separation according to claim 1, which is characterized in that the handle
Diameter is greater than the diameter of sampler barrel.
7. a kind of sampler for biotechnology separation according to claim 1, which is characterized in that the inlet tube
With check valve is equipped on outlet tube.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810492674.6A CN110514488A (en) | 2018-05-22 | 2018-05-22 | A kind of sampler for biotechnology separation |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810492674.6A CN110514488A (en) | 2018-05-22 | 2018-05-22 | A kind of sampler for biotechnology separation |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN110514488A true CN110514488A (en) | 2019-11-29 |
Family
ID=68622213
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810492674.6A Withdrawn CN110514488A (en) | 2018-05-22 | 2018-05-22 | A kind of sampler for biotechnology separation |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN110514488A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111579306A (en) * | 2020-06-20 | 2020-08-25 | 黄凌锋 | Sampling device capable of reducing cell pollution probability in submission process |
| CN111735675A (en) * | 2020-06-05 | 2020-10-02 | 塔里木大学 | Animal tissue sample pretreatment separator |
-
2018
- 2018-05-22 CN CN201810492674.6A patent/CN110514488A/en not_active Withdrawn
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111735675A (en) * | 2020-06-05 | 2020-10-02 | 塔里木大学 | Animal tissue sample pretreatment separator |
| CN111735675B (en) * | 2020-06-05 | 2023-09-29 | 塔里木大学 | Animal tissue sample pretreatment separator |
| CN111579306A (en) * | 2020-06-20 | 2020-08-25 | 黄凌锋 | Sampling device capable of reducing cell pollution probability in submission process |
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|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| WW01 | Invention patent application withdrawn after publication | ||
| WW01 | Invention patent application withdrawn after publication |
Application publication date: 20191129 |