CN111735675B - Animal tissue sample pretreatment separator - Google Patents
Animal tissue sample pretreatment separator Download PDFInfo
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- CN111735675B CN111735675B CN202010515276.9A CN202010515276A CN111735675B CN 111735675 B CN111735675 B CN 111735675B CN 202010515276 A CN202010515276 A CN 202010515276A CN 111735675 B CN111735675 B CN 111735675B
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- treatment cylinder
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/286—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q involving mechanical work, e.g. chopping, disintegrating, compacting, homogenising
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/38—Diluting, dispersing or mixing samples
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/44—Sample treatment involving radiation, e.g. heat
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/286—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q involving mechanical work, e.g. chopping, disintegrating, compacting, homogenising
- G01N2001/2873—Cutting or cleaving
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
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- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Sampling And Sample Adjustment (AREA)
Abstract
The invention relates to the field of biological experiments, and particularly discloses an animal tissue sample pretreatment and separation device which comprises a treatment cylinder, a base, a support plate, a cross beam and a sealing cover; the treatment cylinder is vertically arranged above the base, and is fixedly connected with the base through a plurality of fixed supporting springs on the bottom of the treatment cylinder; rubber material guiding pads with the same shape as the bottom of the treatment cylinder are fixedly arranged on the periphery of the bearing, and are made of elastic materials; a plurality of discharge holes are uniformly formed in the periphery of the bottom of the treatment cylinder; the bottom outside of the treatment cylinder is sleeved with a circle of liquid separation box, and the middle part of the liquid separation box is provided with connectors which face the treatment cylinder and are in one-to-one correspondence with the discharge holes. After the animal tissue sample is processed, the processing cylinder is pressed, so that the internal crushed tissue sample completely enters the liquid separation box, less tissue sample can be processed, the sample is ensured to be processed thoroughly, and the subsequent experimental operation is convenient to use.
Description
Technical Field
The invention relates to the field of biological experiments, in particular to an animal tissue sample pretreatment and separation device.
Background
With the rapid development of life sciences, cells play an increasingly important role in basic scientific research, biological medicine, clinical application and the like. The cells cultured in vitro are isolated from organism tissues, and the traditional method for separating the tissues into single cells comprises a mechanical method, an enzyme digestion method, a combination of the two methods and the like.
When the animal cells are scientifically researched, the animal tissues are required to be sampled, and then the constituent structures in the animal tissues are separated; when obtaining animal tissue, need refine the crushing with animal tissue sample, carry out the preliminary treatment, current preliminary treatment separator is bulky, can't fine to handle to a small amount of animal tissue sample for the treatment is not thorough, influences the separation of each subsequent composition structure, influences the experiment progress.
Disclosure of Invention
The invention aims to provide an animal tissue sample pretreatment and separation device for solving the problems in the background technology.
In order to achieve the above purpose, the present invention provides the following technical solutions:
an animal tissue sample pretreatment and separation device comprises a treatment cylinder, a base, a support plate, a cross beam and a sealing cover; the treatment cylinder is vertically arranged above the base, and is fixedly connected with the base through a plurality of fixed supporting springs on the bottom of the treatment cylinder, and the treatment cylinder is supported by the supporting springs so that the treatment cylinder can move in the vertical direction; a vertical motor coaxial with the treatment cylinder is fixed on a base corresponding to the lower part of the treatment cylinder, a transmission shaft which vertically stretches into the treatment cylinder is coaxially fixed on an output shaft of the motor, the transmission shaft is in sliding connection with the bottom of the treatment cylinder, a horizontal crushing blade is fixed on the top end of the transmission shaft, and the crushing blade is positioned at the lowest position in the treatment cylinder when the treatment cylinder is positioned at the highest point; the bearing is fixedly sleeved on the side wall of the transmission shaft corresponding to the lower part of the crushing blade, and is a sealed bearing, so that sealing is ensured, and liquid is prevented from flowing downwards through the bearing; the periphery of the bearing is fixedly provided with a rubber guide pad which has the same shape as the bottom of the treatment cylinder, the rubber guide pad is made of an elastic material, the crushing blade is stuck to the upper surface of the rubber guide pad when the crushing blade is positioned at the lowest position in the treatment cylinder, the animal tissue sample falling on the rubber guide pad is subjected to rotary cutting crushing through the crushing blade, the rubber guide pad is stretched when the transmission shaft moves upwards relative to the bottom of the treatment cylinder, the rubber guide pad still keeps complete when the crushing blade is positioned at the highest position, the rubber guide pad cannot be pulled and broken, and the processed animal tissue sample flows along the surface to the periphery edge through the upward pulling of the center of the rubber guide pad; a plurality of discharge holes are uniformly formed in the periphery of the bottom of the treatment cylinder, and animal tissue samples after internal treatment are sent out from the discharge holes; the bottom of the liquid separation box is fixedly supported on the base through a fixing frame, the liquid separation box is fixedly supported, and a discharge hole at the bottom of the liquid separation box is horizontally opposite to the upper part of the liquid separation box when the supporting spring supports the treatment cylinder at the highest position; the liquid separation box is used for sealing the discharge port, so that the sample in the treatment cylinder is prevented from flowing out; the middle part of the liquid separation box is provided with connectors facing the treatment barrels and corresponding to the discharge ports one by one, the discharge ports horizontally correspond to the connectors when the treatment barrels move downwards to the lowest part, the discharge ports are communicated with the connectors, and samples in the treatment barrels are sent into the liquid separation box from the discharge ports and the connectors.
Further: the upper surface of the base is fixedly provided with a vertical support plate, the top end of the support plate is fixedly provided with a horizontal cross beam, the cross beam is positioned right above the treatment cylinder, a horizontal rotating shaft is rotatably arranged on the cross beam, a sliding sleeve which rotates around the rotating shaft in the vertical direction is fixedly arranged on the rotating shaft, when the sliding sleeve is positioned at the vertical position, the central shaft of the sliding sleeve coincides with the central shaft of the treatment cylinder, and a sliding rod matched with the sliding sleeve is arranged in the sliding sleeve in a sliding manner; the lower end of the sliding rod is coaxially fixed with a sealing cover matched with the treatment cylinder, and the sealing cover covers the top of the treatment cylinder to seal the top of the treatment cylinder, so that animal tissue samples are treated in a closed environment; when the sealing cover is required to be opened, the sliding rod is lifted upwards.
Further: the sealing cover is of a hemispherical structure.
Further: the upper end of the sliding rod is fixed with a limiting block, so that the sliding rod slides in the sliding sleeve and is prevented from being separated from the sliding sleeve.
Further: the side wall of the liquid separation box facing the treatment cylinder is covered with a sealing gasket, and the sealing between the liquid separation box and the treatment cylinder is maintained.
Further: a filter screen positioned below the connecting port is fixed in the liquid separation box and used for filtering the tissue sample flowing into the liquid separation box; the liquid taking pipe with a valve is communicated with the side wall of the liquid separation box corresponding to the lower part of the filter screen, so that filtered liquid can be conveniently sampled; the top of the liquid separation box is provided with a sampling port with an outer cover, and the sampling port can be opened to directly sample tissue samples in the filter screen.
Further: the bottom downside of the treatment cylinder is fixed with a sealing plate with the side wall surface flush, and the connecting port is sealed by the sealing plate when the discharging port is staggered with the connecting port.
Further: the outside cover of handling section of thick bamboo is equipped with two heating hoods that can outwards expand, and two heating hoods constitute the cyclic annular cover in the outside of handling section of thick bamboo, are provided with heating element on the inner wall of heating hood towards handling section of thick bamboo, realize the heating in the handling section of thick bamboo.
Further: the heating cover is fixedly welded with a swing arm, and the other end of the swing arm is rotatably connected to the support plate through a vertical hinge, so that the heating cover rotates around the hinge. The treatment cylinder is heated by the heating cover, so that a proper environment is provided for pretreatment of animal tissue samples.
Compared with the prior art, the invention has the beneficial effects that: according to the invention, the treatment cylinder capable of moving in the vertical direction is used for pressing the treatment cylinder after the animal tissue sample is treated, so that the internal crushed tissue sample completely enters the liquid separation box, fewer tissue samples can be treated, the sample treatment is ensured to be thorough, and the subsequent experimental operation is convenient; the treatment cylinder is reset under the action of the supporting spring after being pressed, so that the internal seal is kept, and a proper amount of sampling can be performed; the tissue sample and the liquid can be separated in the liquid separation box, and sampling experiments can be respectively carried out; the heating cover which can be sleeved on the treatment cylinder is arranged outside the treatment cylinder, so that a proper environment is provided for pretreatment of animal tissue samples.
Drawings
Fig. 1 is a schematic perspective view of an animal tissue sample pretreatment separator.
FIG. 2 is a schematic view showing the internal structure of the pretreatment cartridge in the device for separating animal tissue samples at the highest point.
FIG. 3 is a schematic view showing the internal structure of the pretreatment cartridge in the device for separating animal tissue samples at the lowest point.
Fig. 4 is a schematic top view of an animal tissue sample pretreatment separator.
In the figure: the device comprises a 1-treatment cylinder, a 2-base, a 3-support plate, a 4-cross beam, a 5-sealing cover, a 6-slide bar, a 7-sliding sleeve, an 8-rotating shaft, a 9-liquid separation box, a 10-fixing frame, an 11-supporting spring, a 12-transmission shaft, a 13-crushing blade, a 14-bearing, a 15-rubber guide pad, a 16-discharge hole, a 17-connection hole, a 18-filter screen, a 19-liquid taking pipe, a 20-sampling hole, a 21-sealing plate, a 22-sealing pad, a 23-heating cover, a 24-swing arm, a 25-hinge and a 26-motor.
Detailed Description
Example 1
Referring to the figure, in the embodiment of the invention, an animal tissue sample pretreatment and separation device comprises a treatment cylinder 1, a base 2, a support plate 3, a cross beam 4 and a sealing cover 5; the treatment cylinder 1 is vertically arranged above the base 2, the bottom of the treatment cylinder 1 is fixedly connected with the base 2 through a plurality of fixed supporting springs 11, and the treatment cylinder 1 is supported by the supporting springs 11 so that the treatment cylinder 1 can move in the vertical direction; the upper surface of the base 2 is fixedly provided with a vertical support plate 3, the top end of the support plate 3 is fixedly provided with a horizontal cross beam 4, the cross beam 4 is positioned right above the treatment cylinder 1, the cross beam 4 is rotatably provided with a horizontal rotating shaft 8, the rotating shaft 8 is fixedly provided with a sliding sleeve 7 rotating around the rotating shaft 8 in the vertical direction, when the sliding sleeve 7 is positioned at the vertical position, the central axis of the sliding sleeve 7 coincides with the central axis of the treatment cylinder 1, a sliding rod 6 matched with the sliding sleeve 7 is arranged in the sliding sleeve 7 in a sliding manner, and the upper end of the sliding rod 6 is fixedly provided with a limiting block, so that the sliding rod 6 slides in the sliding sleeve 7 and is prevented from being separated from the sliding sleeve 7; the lower end of the sliding rod 6 is coaxially fixed with a sealing cover 5 matched with the treatment cylinder 1, the sealing cover 5 is of a hemispherical structure and covers the top of the treatment cylinder 1, the top of the treatment cylinder 1 is sealed, and animal tissue samples are treated in a closed environment; when the sealing cover 5 needs to be opened, the sliding rod 6 is lifted upwards.
A vertical motor 26 coaxial with the treatment cylinder 1 is fixed on the base 2 corresponding to the lower part of the treatment cylinder 1, a transmission shaft 12 which vertically stretches into the treatment cylinder 1 is coaxially fixed on an output shaft of the motor 26, the transmission shaft 12 is in sliding connection with the bottom of the treatment cylinder 1, a horizontal crushing blade 13 is fixed on the top end of the transmission shaft 12, and the crushing blade 13 is positioned at the lowest position in the treatment cylinder 1 when the treatment cylinder 1 is positioned at the highest position; a bearing 14 is fixedly sleeved on the side wall of the transmission shaft 12 corresponding to the lower part of the crushing blade 13, and the bearing 14 is a sealing bearing, so that sealing is ensured, and liquid is prevented from flowing downwards through the bearing 14;
the periphery of the bearing 14 is fixedly provided with a rubber guide pad 15 with the same shape as the bottom of the treatment cylinder 1, the rubber guide pad 15 is made of an elastic material, the crushing blade 13 is stuck to the upper surface of the rubber guide pad 15 when the crushing blade 13 is positioned at the lowest position in the treatment cylinder 1, the animal tissue sample falling on the rubber guide pad 15 is subjected to rotary cutting crushing through the crushing blade 13, the rubber guide pad 15 is stretched when the transmission shaft 12 moves upwards relative to the bottom of the treatment cylinder 1, the rubber guide pad 15 still keeps complete when the crushing blade 13 is positioned at the highest position, the rubber guide pad 15 is not pulled and broken, and the treated animal tissue sample flows to the periphery edge along the surface of the animal tissue sample by being pulled upwards through the center of the rubber guide pad 15; a plurality of discharge holes 16 are uniformly formed in the periphery of the bottom of the treatment cylinder 1, and animal tissue samples after internal treatment are sent out from the discharge holes 16.
A circle of liquid separation box 9 is sleeved on the outer side of the bottom of the treatment cylinder 1, the liquid separation box 9 is attached to the outer wall of the treatment cylinder 1, the bottom of the liquid separation box 9 is fixedly supported on the base 2 through a fixing frame 10, the liquid separation box 9 is fixedly supported, a discharge hole 16 at the bottom of the support spring 11 is horizontally opposite to the upper part of the liquid separation box 9 when the treatment cylinder 1 is at the highest position, and the liquid separation box 9 seals the discharge hole 16 to prevent samples in the treatment cylinder 1 from flowing out; the side wall of the liquid separation box 9 facing the treatment cylinder 1 is covered with a sealing gasket 22, so that the sealing between the liquid separation box and the treatment cylinder 1 is maintained; the middle part of the liquid separation box 9 is provided with connectors 17 which face the treatment barrels 1 and are in one-to-one correspondence with the discharging holes 16, when the treatment barrels 1 move downwards to the lowest, the discharging holes 16 horizontally correspond to the connectors 17, the discharging holes 16 are communicated with the connectors 17, and samples in the treatment barrels 1 are sent into the liquid separation box 9 from the discharging holes 16 and the connectors 17;
a filter screen 18 positioned below the connection port 17 is fixed in the liquid separation tank 9, and filters the tissue sample flowing into the liquid separation tank 9; in order to avoid the connection port 17 from communicating with the outside, a sealing plate 21 with the side wall surface flush with the bottom lower side of the processing cylinder 1 is fixed, and the connection port 17 is sealed by the sealing plate 21 when the discharge port 16 is staggered with the connection port 17; a liquid taking pipe 19 with a valve is communicated with the side wall of the liquid separation box 9 corresponding to the lower part of the filter screen 18, so that filtered liquid can be conveniently sampled; the top of the liquid separation box 9 is provided with a sampling port 20 with an outer cover, and the sampling port 20 can be opened to directly sample tissue samples in the filter screen 18.
When the device is used, the slide bar 6 is lifted upwards, the sealing cover 5 is taken out from the treatment cylinder 1, so that animal tissue samples are filled into the treatment cylinder 1, and then the sealing cover 5 is covered on the treatment cylinder 1 to keep internal sealing; starting a motor 26 at the bottom of the treatment cylinder 1, and driving a transmission shaft 12 to rotate by the motor 26 to enable the internal crushing blade 13 to rotate along with the rotation, so that animal tissues at the bottom of the treatment cylinder 1 are treated and thinned; after the treatment is finished, the treatment cylinder 1 is pressed downwards to enable the whole treatment cylinder 1 to move downwards, at the moment, the center position of the rubber guide pad 15 positioned at the inner bottom of the treatment cylinder 1 is pulled upwards, the rubber guide pad 15 is inclined, tissue samples positioned in the treatment cylinder 1 flow around along the inclined rubber guide pad 15, all tissue samples in the treatment cylinder can be guaranteed to flow downwards, when the treatment cylinder 1 is pressed to the lowest position, the discharge port 16 coincides with the connection port 17, the tissue samples in the treatment cylinder enter the liquid separation box 9, the liquid separation box 9 is filtered and separated by the filter screen 18, and the liquid taking pipe 19 or the sampling port 20 can be opened when the taking is needed.
Example 2
On the basis of the embodiment 1, the outer side of the treatment cylinder 1 is covered with two heating covers 23 which can be unfolded outwards, the two heating covers 23 form an annular sleeve on the outer side of the treatment cylinder 1, and the inner wall of the heating cover 23 facing the treatment cylinder 1 is provided with heating elements to heat the treatment cylinder 1; a swing arm 24 is fixedly welded on the heating cover 23, and the other end of the swing arm 24 is rotatably connected to the support plate 3 through a vertical hinge 25, so that the heating cover 23 rotates around the hinge 25. The treatment canister 1 is heated by the heating mantle 23 to provide a suitable environment for pretreatment of animal tissue samples.
Claims (9)
1. An animal tissue sample pretreatment and separation device comprises a treatment cylinder (1), a base (2), a support plate (3), a cross beam (4) and a sealing cover (5); the treatment cylinder (1) is vertically arranged above the base (2), and the bottom of the treatment cylinder (1) is fixedly connected with the base (2) through a plurality of fixed supporting springs (11); the device is characterized in that a vertical motor (26) coaxial with the treatment cylinder (1) is fixed on a base (2) corresponding to the lower part of the treatment cylinder (1), a transmission shaft (12) vertically extending into the treatment cylinder (1) is coaxially fixed on an output shaft of the motor (26), the transmission shaft (12) is in sliding connection with the bottom of the treatment cylinder (1), a horizontal crushing blade (13) is fixed on the top end of the transmission shaft (12), and the crushing blade (13) is positioned at the lowest position in the treatment cylinder (1) when the treatment cylinder (1) is positioned at the highest point; a bearing (14) is fixedly sleeved on the side wall of the transmission shaft (12) corresponding to the lower part of the crushing blade (13), a rubber material guiding pad (15) with the same shape as the bottom of the treatment cylinder (1) is fixedly arranged on the periphery of the bearing (14), the rubber material guiding pad (15) is made of an elastic material, and the crushing blade (13) is stuck to the upper surface of the rubber material guiding pad (15) when the crushing blade (13) is positioned at the lowest position in the treatment cylinder (1); a plurality of discharge holes (16) are uniformly formed in the periphery of the bottom of the treatment cylinder (1); a circle of liquid separation box (9) is sleeved on the outer side of the bottom of the treatment cylinder (1), the liquid separation box (9) is attached to the outer wall of the treatment cylinder (1), the bottom of the liquid separation box (9) is fixedly supported on the base (2) through a fixing frame (10), and a supporting spring (11) supports a discharge hole (16) at the bottom of the treatment cylinder (1) to be horizontally opposite to the upper part of the liquid separation box (9) when the treatment cylinder (1) is at the highest position; the middle part of the liquid separation box (9) is provided with connectors (17) which face the treatment cylinder (1) and are in one-to-one correspondence with the discharge holes (16); the discharging port (16) is connected with the connecting port (17) when the treatment cylinder (1) moves downwards to the lowest part;
the processing cylinder (1) is pressed downwards, so that the processing cylinder (1) integrally moves downwards, at the moment, the central position of the rubber guide pad (15) positioned at the inner bottom of the processing cylinder (1) is pulled upwards, the rubber guide pad (15) is inclined, and tissue samples positioned in the processing cylinder (1) flow to the periphery along the inclined rubber guide pad (15).
2. The animal tissue sample pretreatment separation device of claim 1, wherein: the upper surface of the base (2) is fixedly provided with a vertical support plate (3), the top end of the support plate (3) is fixedly provided with a horizontal cross beam (4), the cross beam (4) is positioned right above the treatment cylinder (1), the cross beam (4) is rotatably provided with a horizontal rotating shaft (8), the rotating shaft (8) is fixedly provided with a sliding sleeve (7) rotating around the rotating shaft (8) in the vertical direction, when the sliding sleeve (7) is positioned at the vertical position, the central axis of the sliding sleeve (7) coincides with the central axis of the treatment cylinder (1), and a sliding rod (6) matched with the sliding sleeve is arranged in the sliding sleeve (7) in a sliding manner; the lower end of the sliding rod (6) is coaxially fixed with a sealing cover (5) matched with the treatment cylinder (1), and the sealing cover is covered on the top of the treatment cylinder (1).
3. An animal tissue sample pretreatment separation device according to claim 2, wherein: the sealing cover (5) is of a hemispherical structure.
4. An animal tissue sample pretreatment separation device according to claim 2, wherein: the upper end of the sliding rod (6) is fixed with a limiting block.
5. The animal tissue sample pretreatment separation device of claim 1, wherein: the side wall of the liquid separation box (9) facing the treatment cylinder (1) is covered with a sealing gasket (22).
6. The animal tissue sample pretreatment separation device of claim 1, wherein: a filter screen (18) positioned below the connecting port (17) is fixed in the liquid separation box (9); the liquid sampling pipe (19) with a valve is communicated with the side wall of the liquid separation box (9) corresponding to the lower part of the filter screen (18), and a sampling port (20) with an outer cover is arranged on the top of the liquid separation box (9).
7. The animal tissue sample pretreatment separation device of claim 1, wherein: a sealing plate (21) with the side wall surface flush with the bottom lower side of the treatment cylinder (1) is fixed.
8. An animal tissue sample pretreatment separation device according to any of claims 1-7, wherein: the outer side of the treatment cylinder (1) is covered with two heating covers (23) which can be unfolded outwards, and the inner wall of the heating cover (23) facing the treatment cylinder (1) is provided with a heating element.
9. The animal tissue sample pretreatment separation device of claim 8, wherein: the heating cover (23) is fixedly welded with a swing arm (24), and the other end of the swing arm (24) is rotatably connected to the support plate (3) through a vertical hinge (25).
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010515276.9A CN111735675B (en) | 2020-06-05 | 2020-06-05 | Animal tissue sample pretreatment separator |
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| Application Number | Priority Date | Filing Date | Title |
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| CN202010515276.9A CN111735675B (en) | 2020-06-05 | 2020-06-05 | Animal tissue sample pretreatment separator |
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| CN111735675B true CN111735675B (en) | 2023-09-29 |
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| CN111735675A (en) | 2020-10-02 |
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