CN113082184A

CN113082184A - Traditional Chinese medicine composition for resisting cancer and preparation method thereof

Info

Publication number: CN113082184A
Application number: CN202110377749.8A
Authority: CN
Inventors: 杨保金; 李丽娜
Original assignee: Anhui Baohua Pharmaceutical Co ltd
Current assignee: Anhui Baohua Pharmaceutical Co ltd
Priority date: 2021-04-08
Filing date: 2021-04-08
Publication date: 2021-07-09

Abstract

The invention discloses an anti-cancer traditional Chinese medicine composition and a preparation method thereof, and the anti-cancer traditional Chinese medicine composition comprises the following raw materials in parts by weight: 10-15 parts of curcuma zedoary, 10-15 parts of astragalus membranaceus, 10-20 parts of angelica sinensis, 8-10 parts of pseudo-ginseng, 1-5 parts of gynostemma pentaphylla, 5-8 parts of coix seeds, 2-3 parts of nidus vespae and 3-5 parts of radix ranunculi ternati; and the coating solution is prepared in the preparation process, contains chitosan and an intermediate 8, after being absorbed by a human body, positive charges carried on molecules of the coating solution generate electrostatic interaction with cell membranes, so that the structure of biomembrane binding protein is changed, transmembrane channels are opened, and meanwhile, the coating solution can be complexed with calcium ions and magnesium ions in rectal mucous membranes, the permeability of the cell membranes is further increased, the medicine absorption is further thorough, the medicine absorption rate is increased, and the anti-cancer effect is better.

Description

Traditional Chinese medicine composition for resisting cancer and preparation method thereof

Technical Field

The invention relates to the technical field of drug synthesis, in particular to a traditional Chinese medicine composition for resisting cancer and tumor and a preparation method thereof.

Background

Cancer, also known as malignant tumor in medical terminology, is a disease caused by a malfunction in the mechanisms controlling cell growth and proliferation. In addition to uncontrolled growth, cancer cells can also locally invade surrounding normal tissues and even metastasize to other parts of the body via the systemic circulatory or lymphatic systems. At the present stage, for cancer, methods such as surgery, radiotherapy, chemotherapy and the like are generally adopted, but when tumor cells are killed, great pain is caused to cancer patients, so that the patients are weakened in qi and blood, damaged in liver and kidney, greatly reduced in white blood cells in blood and low in immunity, the treatment difficulty is increased, and the pain of the patients is aggravated.

In recent years, the research of traditional Chinese medicine in the aspect of cancer treatment has been greatly developed, especially, the effective components in the traditional Chinese medicine can be extracted in large quantity by applying modern scientific and technological means, the anticancer effect is further improved, but the traditional Chinese medicine composition for resisting cancer and tumor has slow curative effect, and the obvious effect can be achieved after long-time taking.

Disclosure of Invention

The invention aims to provide a traditional Chinese medicine composition for resisting cancer and a preparation method thereof.

The technical problems to be solved by the invention are as follows:

the traditional Chinese medicine composition for resisting cancer and swelling has slow curative effect, and obvious effect can be achieved after long-time administration.

The purpose of the invention can be realized by the following technical scheme:

a traditional Chinese medicine composition for resisting cancer swelling comprises the following raw materials in parts by weight: 10-15 parts of curcuma zedoary, 10-15 parts of astragalus membranaceus, 10-20 parts of angelica sinensis, 8-10 parts of pseudo-ginseng, 1-5 parts of gynostemma pentaphylla, 5-8 parts of coix seeds, 2-3 parts of nidus vespae and 3-5 parts of radix ranunculi ternati;

the anti-cancer traditional Chinese medicine composition is prepared by the following steps:

step S1: cleaning Curcumae rhizoma, radix astragali, radix Angelicae sinensis, Notoginseng radix, herba Gynostemmatis, Coicis semen, nidus Vespae, and radix Ranunculi Ternati, adding into a pulverizing kettle, pulverizing and mixing, and sieving with 80-100 mesh sieve to obtain Chinese medicinal micropowder;

step S2: adding the Chinese medicinal micropowder into sodium carbonate solution with pH of 8-9, decocting for 3-5 times, each time for 1-2 hr, mixing decoctions, concentrating at 60-80 deg.C to relative density of 1.2-1.5 to obtain first concentrated extract;

step S3: mixing the first concentrated extract with 95% ethanol, keeping the temperature at 0-3 deg.C for 10-15h, filtering to obtain filter cake, and concentrating the filtrate to relative density of 1.2-1.3 to obtain second concentrated extract;

step S4: mixing the filter cake obtained in the step S3 with 95% ethanol by volume concentration, preserving the heat for 10-15h at the temperature of 0-3 ℃, filtering, and concentrating the filtrate to the relative density of 1.3-1.5 to prepare a third concentrated leaching paste;

step S5: mixing the second concentrated extract and the third concentrated extract, adding 60% ethanol, performing ultrasonic treatment at frequency of 5-8MHz for 8-10 hr, distilling to remove ethanol, adding deionized water into substrate, mixing, granulating, and sieving with 20 mesh sieve to obtain Chinese medicinal granule;

step S6: coating the Chinese medicinal microparticles with coating solution at rotation speed of 30-40r/min and temperature of 40-50 deg.C, and oven drying to obtain the Chinese medicinal composition for treating cancer swelling.

Further, the dosage ratio of the traditional Chinese medicine micro powder to the sodium carbonate solution is 1g:8mL, the mass fraction of the sodium carbonate solution is 8-15%, the volume ratio of the first concentrated extract to the ethanol in the step S3 is 1:6, and the dosage ratio of the filter cake to the ethanol in the step S4 is 1g:5 mL.

Further, the coating liquid is prepared by the following steps:

step A1: adding acetic anhydride into a reaction kettle, stirring and adding chromic anhydride under the condition that the rotation speed is 150-;

the reaction process is as follows:

step A2: adding sodium alginate into deionized water, stirring until the sodium alginate is completely dissolved, adding sodium periodate, carrying out a light-shielding reaction for 3-5h at the temperature of 20-25 ℃, adding ethylene glycol to stop the reaction for 1.5-3h to prepare sodium alginate oxide, dissolving sodium sulfide into deionized water, adding sulfur powder and sodium hydroxide, stirring for 1-2h at the rotation speed of 150-;

the reaction process is as follows:

step A3: adding sodium alginate oxide, the intermediate 3 and ethanol into a reaction kettle, stirring and adding triethylamine under the condition that the rotation speed is 200-95 ℃ and 300r/min, carrying out reflux reaction for 10-15h at the temperature of 90-95 ℃, distilling to remove the ethanol to obtain an intermediate 4, dissolving glycine into dichloromethane, adding triethylamine, dropwise adding acetic anhydride under the condition of an ice water bath, reacting for 1.5-2h at the temperature of 20-25 ℃ after dropwise adding to obtain an intermediate 5, adding the intermediate 4, the intermediate 5, the acetic anhydride and sodium acetate into the reaction kettle, and reacting for 4-6h at the temperature of 110-120 ℃ to obtain an intermediate 6;

the reaction process is as follows:

step A4: adding the intermediate 6, deionized water and acetone into a reaction kettle, performing reflux reaction for 5-7h under the conditions that the rotation speed is 200-300r/min, the pH value of a reaction solution is 8-9 and the temperature is 60-70 ℃ to obtain an intermediate 7, treating the intermediate 7 with corynebacterium to obtain an intermediate 8, shearing a gelatin solution and a chitosan solution at the rotation speed of 10000r/min for 20-30s, adding a sodium hydroxide solution until the pH value is 5.5-6, adding the intermediate 8, and stirring at the rotation speed of 400-500r/min for 15-20min to obtain the coating solution.

The reaction process is as follows:

further, the dosage ratio of the acetic anhydride and the chromic anhydride in the step A1 is 60mL:12.5g, the dosage ratio of the acetic anhydride, the p-nitrotoluene, the concentrated sulfuric acid and the dropping liquid is 50mL:6.3g:10mL:60mL, the dosage ratio of the intermediate 1, the deionized water, the ethanol and the concentrated sulfuric acid is 5.3g:20mL:20mL:2mL, and the mass fraction of the concentrated sulfuric acid is 95%.

Further, the dosage ratio of sodium alginate, deionized water and sodium periodate in the step A2 is 5g:500mL:1.34g, the dosage ratio of sodium sulfide, deionized water, sulfur powder and sodium hydroxide is 1g:10mL:1.35g:3g, and the dosage ratio of the intermediate 2 and the sodium polysulfide solution is 1g:6 mL.

Further, the mass ratio of the sodium alginate oxide, the intermediate 3 and the triethylamine in the step A3 is 3:1.2:2, the mass ratio of the glycine, the triethylamine and the acetic anhydride is 1g:1.2g:1mL, and the mass ratio of the intermediate 4, the intermediate 5, the acetic anhydride and the sodium acetate is 5:3:2: 1.5.

Further, the using amount ratio of the intermediate 6, the deionized water and the acetone in the step A4 is 3g:6mL:5mL, the mass fraction of the gelatin solution is 25, and the mass fraction of the chitosan solution is 2.5%.

The preparation method of the traditional Chinese medicine composition for resisting cancer tumor specifically comprises the following steps:

The invention has the beneficial effects that: the invention selects a large amount of traditional Chinese medicines with anti-cancer and anti-tumor functions in the process of preparing the traditional Chinese medicine composition, extracts the traditional Chinese medicines to enable anti-cancer and anti-tumor components contained in the traditional Chinese medicines to be absorbed more easily, and prepares a coating liquid in the process of preparing the traditional Chinese medicine composition with anti-cancer and anti-tumor functions, the coating liquid takes para-nitrotoluene as a raw material to react with dropping liquid to prepare an intermediate 1, the intermediate 1 reacts with concentrated sulfuric acid to prepare an intermediate 2, sodium alginate is oxidized by sodium periodate to prepare oxidized sodium alginate, sodium sulfide is treated by sulfur powder and sodium hydroxide to prepare a sodium polysulfide solution, the intermediate 2 is treated by the sodium polysulfide solution to prepare an intermediate 3, the oxidized sodium alginate and the intermediate 3 are reacted under the action of triethylamine to prepare an intermediate 4, glycine is reacted with acetic anhydride to prepare an intermediate 5, reacting the intermediate 4 with the intermediate 5 to obtain an intermediate 6, further processing the intermediate 6 to obtain an intermediate 7, carrying out enzymatic reaction on the intermediate 7 by corynebacterium through acyl transfer and amino transfer to generate an intermediate 8, and mixing the intermediate 8 with a gelatin solution and a chitosan solution to obtain a coating solution; this coating liquid contains chitosan and intermediate 8 and after the human body absorbs, the positive charge that carries on its molecule produces electrostatic action with the cell membrane, makes the structure of biomembrane binding protein change to lead to the opening of transmembrane channel, can complex with calcium ion and magnesium ion in the rectum mucous membrane simultaneously, and then increase the cell membrane permeability, and then make absorptive more thoroughly of medicine, increase the medicine absorptivity, make anti cancer effect better.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

Example 1

A traditional Chinese medicine composition for resisting cancer swelling comprises the following raw materials in parts by weight: 10 parts of curcuma zedoary, 10 parts of astragalus membranaceus, 10 parts of angelica sinensis, 8 parts of pseudo-ginseng, 1 part of gynostemma pentaphylla, 5 parts of coix seeds, 2 parts of nidus vespae and 3 parts of radix ranunculi ternati;

step S1: cleaning Curcumae rhizoma, radix astragali, radix Angelicae sinensis, Notoginseng radix, herba Gynostemmatis, Coicis semen, nidus Vespae, and radix Ranunculi Ternati, adding into a pulverizing kettle, pulverizing and mixing, and sieving with 80 mesh sieve to obtain Chinese medicinal micropowder;

step S2: adding the Chinese medicinal micropowder into sodium carbonate solution with pH of 8, decocting for 3 times (each time for 1 hr), mixing decoctions, and concentrating at 60 deg.C until the relative density is 1.2 to obtain first concentrated extract;

step S3: mixing the first concentrated extract with 95% ethanol, keeping the temperature at 0 deg.C for 10h, filtering to obtain filter cake, and concentrating the filtrate to relative density of 1.2 to obtain second concentrated extract;

step S4: mixing the filter cake obtained in the step S3 with 95% ethanol by volume concentration, preserving the heat for 10 hours at the temperature of 0 ℃, filtering, and concentrating the filtrate to the relative density of 1.3 to prepare a third concentrated extract;

step S5: mixing the second concentrated extract and the third concentrated extract, adding 60% ethanol, performing ultrasonic treatment at 5MHz for 8 hr, distilling to remove ethanol, adding deionized water into substrate, mixing, granulating, and sieving with 20 mesh sieve to obtain Chinese medicinal granule;

step S6: coating the Chinese medicinal particles with coating solution at 40 deg.C at rotation speed of 30r/min, and drying to obtain the Chinese medicinal composition for treating cancer.

The coating liquid is prepared by the following steps:

step A1: adding acetic anhydride into a reaction kettle, stirring and adding chromic anhydride under the condition that the rotating speed is 150r/min to prepare dropping liquid, adding the acetic anhydride and p-nitrotoluene into the reaction kettle, adding concentrated sulfuric acid under the condition that the temperature is 0 ℃, stirring and dropping the dropping liquid under the condition that the rotating speed is 200r/min, reacting for 2 hours under the condition that the temperature is 5 ℃ to prepare an intermediate 1, adding the intermediate 1, deionized water, ethanol and concentrated sulfuric acid into the reaction kettle, and refluxing for 30 minutes under the condition that the temperature is 100 ℃ to prepare an intermediate 2;

step A2: adding sodium alginate into deionized water, stirring until the sodium alginate is completely dissolved, adding sodium periodate, carrying out a light-shielding reaction for 3 hours at the temperature of 20 ℃, adding ethylene glycol to stop the reaction for 1.5 hours to prepare sodium alginate oxide, dissolving sodium sulfide into deionized water, adding sulfur powder and sodium hydroxide, stirring for 1 hour at the rotation speed of 150r/min and the temperature of 70 ℃ to prepare a sodium polysulfide solution, dissolving an intermediate 2 into benzene, and dropwise adding the sodium polysulfide solution at the temperature of 80 ℃ to carry out a reaction for 3 hours to prepare an intermediate 3;

step A3: adding sodium alginate oxide, the intermediate 3 and ethanol into a reaction kettle, stirring and adding triethylamine under the condition that the rotating speed is 200r/min, carrying out reflux reaction for 10 hours at the temperature of 90 ℃, distilling to remove the ethanol to obtain an intermediate 4, dissolving glycine into dichloromethane, adding triethylamine, dropwise adding acetic anhydride under the condition of an ice water bath, reacting for 1.5 hours at the temperature of 20 ℃ after dropwise adding to obtain an intermediate 5, adding the intermediate 4, the intermediate 5, the acetic anhydride and sodium acetate into the reaction kettle, and reacting for 4 hours at the temperature of 110 ℃ to obtain an intermediate 6;

step A4: adding the intermediate 6, deionized water and acetone into a reaction kettle, performing reflux reaction for 5 hours at the rotation speed of 200r/min, the pH value of a reaction solution of 8 and the temperature of 60 ℃ to obtain an intermediate 7, treating the intermediate 7 with corynebacterium to obtain an intermediate 8, shearing a gelatin solution and a chitosan solution at the rotation speed of 10000r/min for 20 seconds, adding a sodium hydroxide solution until the pH value is 5.5, adding the intermediate 8, and stirring at the rotation speed of 400r/min for 15 minutes to obtain a coating solution.

Example 2

A traditional Chinese medicine composition for resisting cancer swelling comprises the following raw materials in parts by weight: 13 parts of curcuma zedoary, 13 parts of astragalus, 15 parts of angelica, 9 parts of pseudo-ginseng, 3 parts of gynostemma pentaphylla, 6 parts of coix seed, 2.5 parts of nidus vespae and 4 parts of radix ranunculi ternati;

step S2: adding the Chinese medicinal micropowder into sodium carbonate solution with pH of 9, decocting for 3 times, each time for 2 hr, mixing decoctions, concentrating at 60 deg.C to relative density of 1.5 to obtain first concentrated extract;

step S3: mixing the first concentrated extract with 95% ethanol, keeping the temperature at 0 deg.C for 15h, filtering to obtain filter cake, and concentrating the filtrate to relative density of 1.2 to obtain second concentrated extract;

step S4: mixing the filter cake obtained in the step S3 with 95% ethanol by volume concentration, preserving the heat for 10 hours at the temperature of 3 ℃, filtering, and concentrating the filtrate to the relative density of 1.5 to prepare a third concentrated extract;

step S5: mixing the second concentrated extract and the third concentrated extract, adding 60% ethanol, performing ultrasonic treatment at 5MHz for 10 hr, distilling to remove ethanol, adding deionized water into substrate, mixing, granulating, and sieving with 20 mesh sieve to obtain Chinese medicinal granule;

step S6: coating the Chinese medicinal particles with coating solution at rotation speed of 30r/min and temperature of 50 deg.C, and oven drying to obtain the Chinese medicinal composition for treating cancer.

The coating liquid is prepared by the following steps:

step A1: adding acetic anhydride into a reaction kettle, stirring and adding chromic anhydride under the condition that the rotating speed is 150r/min to prepare dropping liquid, adding the acetic anhydride and p-nitrotoluene into the reaction kettle, adding concentrated sulfuric acid under the condition that the temperature is 0 ℃, stirring and dropping the dropping liquid under the condition that the rotating speed is 300r/min, reacting for 3 hours under the condition that the temperature is 5 ℃ to prepare an intermediate 1, adding the intermediate 1, deionized water, ethanol and concentrated sulfuric acid into the reaction kettle, and refluxing for 40 minutes under the condition that the temperature is 100 ℃ to prepare an intermediate 2;

step A2: adding sodium alginate into deionized water, stirring until the sodium alginate is completely dissolved, adding sodium periodate, carrying out a light-shielding reaction for 5 hours at the temperature of 20 ℃, adding ethylene glycol to stop the reaction for 1.5 hours to prepare sodium alginate oxide, dissolving sodium sulfide into deionized water, adding sulfur powder and sodium hydroxide, stirring for 2 hours at the rotation speed of 200r/min and the temperature of 70 ℃ to prepare a sodium polysulfide solution, dissolving an intermediate 2 into benzene, and dropwise adding the sodium polysulfide solution at the temperature of 80 ℃ to carry out a reaction for 5 hours to prepare an intermediate 3;

step A3: adding sodium alginate oxide, the intermediate 3 and ethanol into a reaction kettle, stirring and adding triethylamine under the condition that the rotating speed is 200r/min, carrying out reflux reaction for 10 hours at the temperature of 95 ℃, distilling to remove the ethanol to obtain an intermediate 4, dissolving glycine into dichloromethane, adding triethylamine, dropwise adding acetic anhydride under the condition of an ice water bath, reacting for 1.5 hours at the temperature of 25 ℃ after dropwise adding to obtain an intermediate 5, adding the intermediate 4, the intermediate 5, the acetic anhydride and sodium acetate into the reaction kettle, and reacting for 4 hours at the temperature of 120 ℃ to obtain an intermediate 6;

step A4: adding the intermediate 6, deionized water and acetone into a reaction kettle, performing reflux reaction for 5 hours at the rotation speed of 300r/min, the pH value of a reaction solution of 8 and the temperature of 70 ℃ to obtain an intermediate 7, treating the intermediate 7 with corynebacterium to obtain an intermediate 8, shearing a gelatin solution and a chitosan solution at the rotation speed of 10000r/min for 30 seconds, adding a sodium hydroxide solution until the pH value is 5.5, adding the intermediate 8, and stirring at the rotation speed of 500r/min for 15 minutes to obtain a coating solution.

Example 3

A traditional Chinese medicine composition for resisting cancer swelling comprises the following raw materials in parts by weight: 15 parts of curcuma zedoary, 15 parts of astragalus membranaceus, 20 parts of angelica sinensis, 10 parts of pseudo-ginseng, 5 parts of gynostemma pentaphylla, 8 parts of coix seeds, 3 parts of nidus vespae and 5 parts of radix ranunculi ternati;

step S1: cleaning Curcumae rhizoma, radix astragali, radix Angelicae sinensis, Notoginseng radix, herba Gynostemmatis, Coicis semen, nidus Vespae, and radix Ranunculi Ternati, adding into a pulverizing kettle, pulverizing and mixing, and sieving with 100 mesh sieve to obtain Chinese medicinal micropowder;

step S2: adding the Chinese medicinal micropowder into sodium carbonate solution with pH of 9, decocting for 5 times, each time for 2 hr, mixing decoctions, concentrating at 80 deg.C to relative density of 1.5 to obtain first concentrated extract;

step S3: mixing the first concentrated extract with 95% ethanol, keeping the temperature at 3 deg.C for 15h, filtering to obtain filter cake, and concentrating the filtrate to relative density of 1.3 to obtain second concentrated extract;

step S4: mixing the filter cake obtained in the step S3 with 95% ethanol by volume concentration, preserving the heat for 15 hours at the temperature of 3 ℃, filtering, and concentrating the filtrate to the relative density of 1.5 to prepare a third concentrated extract;

step S5: mixing the second concentrated extract and the third concentrated extract, adding 60% ethanol, performing ultrasonic treatment at frequency of 8MHz for 10 hr, distilling to remove ethanol, adding deionized water into substrate, mixing, granulating, and sieving with 20 mesh sieve to obtain Chinese medicinal granule;

step S6: coating the Chinese medicinal particles with coating solution at rotation speed of 40r/min and temperature of 50 deg.C, and oven drying to obtain the Chinese medicinal composition for treating cancer.

The coating liquid is prepared by the following steps:

step A1: adding acetic anhydride into a reaction kettle, stirring and adding chromic anhydride under the condition that the rotating speed is 200r/min to prepare dropping liquid, adding the acetic anhydride and p-nitrotoluene into the reaction kettle, adding concentrated sulfuric acid under the condition that the temperature is 0 ℃, stirring and dropping the dropping liquid under the condition that the rotating speed is 300r/min, reacting for 3 hours under the condition that the temperature is 10 ℃ to prepare an intermediate 1, adding the intermediate 1, deionized water, ethanol and concentrated sulfuric acid into the reaction kettle, and refluxing for 40 minutes under the condition that the temperature is 110 ℃ to prepare an intermediate 2;

step A2: adding sodium alginate into deionized water, stirring until the sodium alginate is completely dissolved, adding sodium periodate, carrying out a light-shielding reaction for 5 hours at the temperature of 25 ℃, adding ethylene glycol to stop the reaction for 3 hours to prepare sodium alginate oxide, dissolving sodium sulfide into deionized water, adding sulfur powder and sodium hydroxide, stirring for 2 hours at the rotation speed of 200r/min and the temperature of 80 ℃ to prepare a sodium polysulfide solution, dissolving an intermediate 2 into benzene, dropwise adding the sodium polysulfide solution at the temperature of 85 ℃ to carry out a reaction for 5 hours to prepare an intermediate 3;

step A3: adding sodium alginate oxide, the intermediate 3 and ethanol into a reaction kettle, stirring and adding triethylamine under the condition that the rotating speed is 300r/min, carrying out reflux reaction for 15h at the temperature of 95 ℃, distilling to remove the ethanol to obtain an intermediate 4, dissolving glycine into dichloromethane, adding triethylamine, dropwise adding acetic anhydride under the condition of an ice water bath, reacting for 2h at the temperature of 25 ℃ after dropwise adding is finished to obtain an intermediate 5, adding the intermediate 4, the intermediate 5, the acetic anhydride and sodium acetate into the reaction kettle, and reacting for 6h at the temperature of 120 ℃ to obtain an intermediate 6;

step A4: adding the intermediate 6, deionized water and acetone into a reaction kettle, performing reflux reaction for 7 hours at the rotation speed of 300r/min, the pH value of a reaction solution of 9 and the temperature of 70 ℃ to obtain an intermediate 7, treating the intermediate 7 with corynebacterium to obtain an intermediate 8, shearing a gelatin solution and a chitosan solution at the rotation speed of 10000r/min for 30 seconds, adding a sodium hydroxide solution until the pH value is 6, adding the intermediate 8, and stirring at the rotation speed of 500r/min for 20 minutes to obtain a coating solution.

Comparative example 1

The coating solution of the comparative example and the example 1 does not contain the intermediate 8, and specifically comprises the following steps:

The coating liquid is prepared by the following steps:

shearing the gelatin solution and the chitosan solution for 20s at the rotation speed of 10000r/min, adding the sodium hydroxide solution until the pH value is 5.5, and stirring for 15min at the rotation speed of 400r/min to obtain the coating solution.

Comparative example 2

Compared with the example 1, the comparative example does not use the coating solution of the example 1, and specifically comprises the following steps:

The coating liquid is prepared by the following steps:

shearing and emulsifying gelatin solution and angelica oil by mass fraction for 20s under 10000r/min, adding chitosan acetate solution, stirring at the rotation speed of 400r/min and the temperature of 50 ℃ for 20min, adding deionized water to dilute for one time, adding sodium hydroxide solution with the mass fraction of 2%, and continuously stirring to obtain the coating solution.

Comparative example 3

The comparative example is a common traditional Chinese medicine composition for resisting cancer tumor in the market.

Test example 1

If the patient Li is taken twice a day, three capsules are taken each time for 3 months, all symptoms are improved.

Test example 2

The patients had confirmed diagnosis of liver cancer by a certain diagnosis in week, and had symptoms of dysphagia and epigastric distending pain and cough after 5 times of postoperative chemotherapy, and all symptoms were improved by eating the anti-cancer traditional Chinese medicine composition prepared in example 2 twice a day, three granules each time, and continuously taking for 2 months.

Test example 3

The patient Wangzou has confirmed diagnosis of esophageal cancer, chemotherapy is carried out for 8 times after operation, severe vomiting, dizziness and alopecia are caused in the chemotherapy process, and after the anti-cancer traditional Chinese medicine composition prepared in the embodiment 3 is eaten twice a day, three grains are taken every time, and the oral administration is continuously carried out for 4 months, all symptoms are improved.

Test example 4

The patient has confirmed diagnosis of breast cancer, 20 times of postoperative radiotherapy and symptoms of hydrothorax in the radiotherapy process, and all symptoms are improved by eating the anti-cancer traditional Chinese medicine composition prepared in the comparative example 1 twice a day, three capsules each time and continuously taking for 8 months.

Test example 5

The patient had a definite diagnosis of nasopharyngeal carcinoma, and had 3 times of postoperative chemotherapy, and severe vomiting and white sticky foam during the chemotherapy, and all symptoms were improved by eating the anti-cancer traditional Chinese medicine composition prepared in comparative example 2 twice a day, three granules each time, and continuously taking for 7 months.

Test example 6

After the patient takes the anti-cancer traditional Chinese medicine composition prepared in the example 3 twice a day, three capsules each time and 8 months of continuous administration, all symptoms are improved.

From the above test examples 1-6, it can be seen that the anticancer Chinese medicinal composition prepared by the present invention has a faster effect on the treatment of cancer.

The foregoing is merely exemplary and illustrative of the principles of the present invention and various modifications, additions and substitutions of the specific embodiments described herein may be made by those skilled in the art without departing from the principles of the present invention or exceeding the scope of the claims set forth herein.

Claims

1. A traditional Chinese medicine composition for resisting cancer tumor is characterized in that: the feed comprises the following raw materials in parts by weight: 10-15 parts of curcuma zedoary, 10-15 parts of astragalus membranaceus, 10-20 parts of angelica sinensis, 8-10 parts of pseudo-ginseng, 1-5 parts of gynostemma pentaphylla, 5-8 parts of coix seeds, 2-3 parts of nidus vespae and 3-5 parts of radix ranunculi ternati;

processing the above raw materials to obtain Chinese medicinal microparticles, and coating the Chinese medicinal microparticles with coating solution to obtain anticancer and repercussive Chinese medicinal composition;

the coating liquid is prepared by the following steps:

step A1: mixing acetic anhydride and chromic anhydride to prepare dropping liquid, adding the acetic anhydride and p-nitrotoluene into a reaction kettle, adding concentrated sulfuric acid, stirring, dropwise adding the dropping liquid, reacting to prepare an intermediate 1, adding the intermediate 1, deionized water, ethanol and concentrated sulfuric acid into the reaction kettle, and refluxing to prepare an intermediate 2;

step A2: dissolving sodium alginate in deionized water, adding sodium periodate, carrying out a light-resistant reaction, adding ethylene glycol to terminate the reaction to obtain sodium alginate oxide, dissolving sodium sulfide in deionized water, adding sulfur powder and sodium hydroxide to obtain a sodium polysulfide solution, dissolving the intermediate 2 in benzene, dropwise adding the sodium polysulfide solution, and carrying out a reaction to obtain an intermediate 3;

step A3: adding sodium alginate oxide, the intermediate 3 and ethanol into a reaction kettle, stirring, adding triethylamine, performing reflux reaction to obtain an intermediate 4, dissolving glycine into dichloromethane, adding triethylamine, dropwise adding acetic anhydride under the condition of ice-water bath, reacting to obtain an intermediate 5, adding the intermediate 4, the intermediate 5, the acetic anhydride and sodium acetate into the reaction kettle, and reacting to obtain an intermediate 6;

step A4: adding the intermediate 6, deionized water and acetone into a reaction kettle, carrying out reflux reaction to obtain an intermediate 7, treating the intermediate 7 with corynebacterium to obtain an intermediate 8, shearing a gelatin solution and a chitosan solution, adding a sodium hydroxide solution, adding the intermediate 8, and stirring to obtain a coating solution.

2. The traditional Chinese medicine composition for treating cancer according to claim 1, which is characterized in that: the dosage ratio of the acetic anhydride to the chromic anhydride in the step A1 is 60mL to 12.5g, the dosage ratio of the acetic anhydride, the p-nitrotoluene, the concentrated sulfuric acid and the dropping liquid is 50mL to 6.3g to 10mL to 60mL, the dosage ratio of the intermediate 1, the deionized water, the ethanol and the concentrated sulfuric acid is 5.3g to 20mL to 2mL, and the mass fraction of the concentrated sulfuric acid is 95%.

3. The traditional Chinese medicine composition for treating cancer according to claim 1, which is characterized in that: the dosage ratio of the sodium alginate, the deionized water and the sodium periodate in the step A2 is 5g:500mL:1.34g, the dosage ratio of the sodium sulfide, the deionized water, the sulfur powder and the sodium hydroxide is 1g:10mL:1.35g:3g, and the dosage ratio of the intermediate 2 and the sodium polysulfide solution is 1g:6 mL.

4. The traditional Chinese medicine composition for treating cancer according to claim 1, which is characterized in that: the mass ratio of the sodium alginate oxide, the intermediate 3 and the triethylamine in the step A3 is 3:1.2:2, the mass ratio of the glycine, the triethylamine and the acetic anhydride is 1g:1.2g:1mL, and the mass ratio of the intermediate 4, the intermediate 5, the acetic anhydride and the sodium acetate is 5:3:2: 1.5.

5. The traditional Chinese medicine composition for treating cancer according to claim 1, which is characterized in that: the dosage ratio of the intermediate 6, the deionized water and the acetone in the step A4 is 3g, 6mL and 5mL, the mass fraction of the gelatin solution is 25%, and the mass fraction of the chitosan solution is 2.5%.

6. The method for preparing a Chinese medicinal composition for treating cancer according to claim 1, wherein the Chinese medicinal composition comprises the following raw materials: the method specifically comprises the following steps:

step S1: cleaning Curcumae rhizoma, radix astragali, radix Angelicae sinensis, Notoginseng radix, herba Gynostemmatis, Coicis semen, nidus Vespae, and radix Ranunculi Ternati, pulverizing, mixing, and sieving with 80-100 mesh sieve to obtain Chinese medicinal micropowder;

step S4: mixing the filter cake obtained in the step S3 with 95% ethanol, preserving the temperature for 10-15h at the temperature of 0-3 ℃, filtering, and concentrating the filtrate to the relative density of 1.3-1.5 to prepare a third concentrated leaching paste;