CN113081906A

CN113081906A - Astaxanthin composition and preparation method and application thereof

Info

Publication number: CN113081906A
Application number: CN202110478349.6A
Authority: CN
Inventors: 张金金; 林娜妹; 张慧; 李传茂; 张伟杰; 张楚标
Original assignee: Guangdong Danz Group Co Ltd; Guangzhou Keneng Cosmetic Research Co Ltd
Current assignee: Guangdong Danz Group Co Ltd; Guangzhou Keneng Cosmetic Research Co Ltd
Priority date: 2021-04-30
Filing date: 2021-04-30
Publication date: 2021-07-09
Anticipated expiration: 2041-04-30
Also published as: WO2022228268A1; CN113081906B

Abstract

The invention relates to an astaxanthin composition and a preparation method and application thereof. The astaxanthin composition comprises the following components in percentage by mass: microalgae oil: 25 to 50 percent; oat kernel oil: 45 to 70 percent; astaxanthin: 0.01 to 5 percent. Compared with the prior art, the invention has the beneficial effects that: according to the high-efficiency stable astaxanthin composition provided by the invention, the microalgae oil and the oat kernel oil are compounded and used in an astaxanthin-containing antioxidant skin care product, so that the astaxanthin has good light stability and thermal stability, and the antioxidant activity of the astaxanthin in the skin care product is maintained.

Description

Astaxanthin composition and preparation method and application thereof

Technical Field

The invention relates to an astaxanthin composition and a preparation method and application thereof, in particular to an efficient and stable astaxanthin composition and a preparation method and application thereof, belonging to the field of cosmetics.

Background

With the skin aging problem being highly valued by consumers, the demand of antioxidant skin care products is continuously increasing, while astaxanthin belongs to one of carotenoids, has a strong antioxidant, and is well accepted by consumers as an ingredient in skin care products. However, astaxanthin contains a plurality of long conjugated unsaturated double bonds in the molecular structure, is sensitive to light and heat, is extremely unstable in property and easy to fade, so that the stability of astaxanthin in cosmetic application is influenced, and the antioxidant performance is reduced.

In recent years, the stability of astaxanthin has been studied in many cases, but it has been reported that the preservation of astaxanthin is facilitated by drying by lyophilization, low-temperature storage under sealed conditions, vacuum refrigeration, and the like, in view of the preservation of astaxanthin as a raw material. However, there have been few studies on the application of astaxanthin to skin care products to maintain good activity under heat and light. Therefore, how to keep the astaxanthin applied in the antioxidant skin care product does not fade under the conditions of light and heat, and the maintenance of good activity in the shelf life becomes a great technical difficulty.

Citation 1 discloses a high-efficiency stable light-resistant and oxidation-resistant composition and application thereof, wherein the composition comprises the following components in parts by weight: 0.0001-0.2 part of astaxanthin; 0.01-2.0 parts of glucosyl rutin. But the glucosyl rutin and the water-soluble astaxanthin are compounded, so that the skin care product has good photo-thermal stability when being applied to the skin care product, but the glucosyl rutin can absorb blue light under illumination, and the body color can turn yellow along with the prolonging of the shelf life.

Reference 2 discloses an astaxanthin-containing aqueous composition containing at least (a) astaxanthin, (B) iron of 20 μ g/L or more, and (C) an iron chelator; also disclosed is a method for suppressing the decomposition of astaxanthin, wherein an iron chelate agent (C) is contained in an aqueous composition containing at least (A) astaxanthin and (8)20 [ mu ] g/L or more of iron. The astaxanthin-containing water-based composition contains iron ions at a certain concentration, and may not be compatible with a thickener in a cosmetic formula or cause discoloration of active ingredients, and the water-based composition protects astaxanthin from degradation by a mechanism of blocking the influence of components in a skin care water system on astaxanthin.

Citations

Cited document 1: CN111557861A

Cited document 2: CN103025306A

Disclosure of Invention

Problems to be solved by the invention

In view of the technical problems in the prior art, the invention firstly provides an astaxanthin composition, which is prepared by compounding microalgae oil and oat kernel oil, so that the astaxanthin has good stability of heat resistance and light resistance in skin care products or cosmetics, and the antioxidant activity of the astaxanthin in the skin care products is maintained.

The invention also provides an emulsifying system which has strong antioxidant activity and excellent thermal stability and illumination stability.

Means for solving the problems

The invention provides an astaxanthin composition, which comprises the following components in percentage by mass:

microalgae oil: 25 to 50 percent;

oat kernel oil: 45 to 70 percent;

astaxanthin: 0.01 to 5 percent.

The astaxanthin composition comprises 30-50% of microalgae oil, 50-70% of oat kernel oil and 0.05-5% of astaxanthin by mass.

The astaxanthin composition comprises the microalgae oil, oat kernel oil and astaxanthin, wherein the weight ratio of the microalgae oil to the oat kernel oil to the astaxanthin is 1 (0.9-2.8) to (0.0002-0.2); preferably 1 (1-2.3) and 0.0017-0.2.

The invention also provides a preparation method of the astaxanthin composition, which comprises the step of mixing the components of the astaxanthin composition.

The invention also provides an emulsification system comprising an astaxanthin composition according to the invention; preferably, the astaxanthin composition is added in an amount of 0.01-10% by mass based on the total mass of the emulsifying system.

The emulsifying system further comprises one or more of an emulsifier, grease, a thickener, a humectant and a preservative.

The emulsifying system provided by the invention is characterized in that the addition amount of the emulsifying agent is 0.1-5% of the total mass of the emulsifying system; the addition amount of the grease is 0.1-40%; the addition amount of the thickening agent is 0.01-2%; the addition amount of the humectant is 0.01-20%; the addition amount of the preservative is 0.01-2%.

The invention also provides a method for preparing the emulsifying system, which comprises the step of mixing the components of the emulsifying system.

The invention further provides an astaxanthin composition according to the invention or a use of an astaxanthin composition according to the invention in skin care products or cosmetics.

The skin care product or the cosmetic comprises one or more of emulsion, essence, sleep mask, cream, skin oil, massage cream, lipstick and lipstick.

ADVANTAGEOUS EFFECTS OF INVENTION

Compared with the prior art, the invention has the beneficial effects that: according to the high-efficiency stable astaxanthin composition provided by the invention, the microalgae oil and the oat kernel oil are compounded and used in an astaxanthin-containing antioxidant skin care product, so that the astaxanthin has good light stability and thermal stability, and the antioxidant activity of the astaxanthin in the skin care product is maintained.

The astaxanthin composition is added into the emulsifying system, and the emulsifying system has excellent antioxidant activity, heat stability and light stability.

The astaxanthin composition can be applied to skin care products or cosmetics.

Drawings

FIG. 1 shows the DPPH clearance under various conditions for emulsions prepared according to application examples 1,3 to 4 and comparative application examples 1 to 3 and for emulsions of a control.

Detailed Description

Various exemplary embodiments, features and aspects of the invention will be described in detail below. The word "exemplary" is used exclusively herein to mean "serving as an example, embodiment, or illustration. Any embodiment described herein as "exemplary" is not necessarily to be construed as preferred or advantageous over other embodiments.

Furthermore, in the following detailed description, numerous specific details are set forth in order to provide a better understanding of the present invention. It will be understood by those skilled in the art that the present invention may be practiced without some of these specific details. In other instances, methods, means, devices and steps which are well known to those skilled in the art have not been described in detail so as not to obscure the invention.

All units used in the specification are international standard units unless otherwise stated, and numerical values and numerical ranges appearing in the present invention should be understood to include systematic errors inevitable in industrial production.

In the present specification, "%" denotes mass% unless otherwise specified.

In the present specification, the meaning of "may" includes both the meaning of performing a certain process and the meaning of not performing a certain process.

In the present specification, reference to "some particular/preferred embodiments," "other particular/preferred embodiments," "embodiments," and the like, means that a particular element (e.g., feature, structure, property, and/or characteristic) described in connection with the embodiment is included in at least one embodiment described herein, and may or may not be present in other embodiments. In addition, it is to be understood that the described elements may be combined in any suitable manner in the various embodiments.

In the present specification, the numerical range represented by "numerical value a to numerical value B" means a range including the end point numerical value A, B.

In the present specification, the term "water" includes any available water that can be used in the cosmetic field, such as deionized water, distilled water, ion-exchanged water, double distilled water, high purity water, and purified water.

In the present specification, the specific temperature may be 10 to 40 ℃ if "room temperature" or "normal temperature" is concerned.

The first aspect of the invention provides an astaxanthin composition, which comprises the following components in percentage by mass based on the total mass of the astaxanthin composition:

microalgae oil: 25 to 50 percent;

oat kernel oil: 45 to 70 percent;

astaxanthin: 0.01 to 5 percent.

The astaxanthin composition disclosed by the invention enables the astaxanthin to have good light stability and thermal stability in skin care products, and meanwhile, good antioxidant activity is kept.

Microalgae oil

The microalgae oil in the astaxanthin composition is natural oil extracted from microalgae organisms, is rich in Omega-9 unsaturated fatty acid beneficial to human bodies, and has stable properties and strong oxidation resistance. Preferably, the microalgae oil is a transparent light yellow liquid oil extracted after chestnut tree juice is separated and screened, and dried cane sugar is used as carbonic acid for fermentation, is rich in more than 90% of Omega-9 monounsaturated fatty acid (oleic acid), has excellent oxidation resistance, is stable in property and is not easy to discolor.

The addition amount of the microalgae oil is 25-50%, preferably 30-50% based on the total mass of the astaxanthin composition, such as: the amount of microalgae oil added may be 26%, 27%, 28%, 29%, 30%, 32%, 33%, 35%, 36%, 38%, 40%, 42%, 43%, 45%, etc. When the addition amount of the microalgae oil is 25-50%, the light-resistant and heat-resistant properties of the astaxanthin in the application of skin care products can be remarkably improved, and the excellent antioxidant activity is kept.

Oat kernel oil

The oat kernel oil in the astaxanthin composition of the present invention is an oil refined from oat kernels. Preferably, the oat kernel oil of the present invention may use CO₂The natural pure vegetable oil obtained by supercritical extraction without solvent is yellow and transparent, and is rich in more than 80% of unsaturated fatty acid and various active ingredients, such as natural antioxidant ingredients such as Omega-6 linoleic acid, Omega-3 linolenic acid, Omega-9 oleic acid, vitamin E, and the like.

The addition amount of the oat kernel oil is 45-70% by mass, preferably 50-70% by mass, based on the total mass of the astaxanthin composition, for example: may be 46%, 48%, 50%, 52%, 55%, 58%, 60%, 62%, 63%, 65%, 68%, 69%, etc. The addition amount of the oat kernel oil is 45-70%, and the oat kernel oil can generate a synergistic interaction effect with the microalgae oil, so that the UV protection stability of the astaxanthin in the application of skin care products is improved, and excellent antioxidant activity can be exerted.

Astaxanthin

In the present invention, "astaxanthin" includes astaxanthin and derivatives such as esters of astaxanthin (hereinafter, also referred to as "astaxanthin" as appropriate).

The astaxanthin in the present invention may be any astaxanthin as long as it is obtained by a conventional method, in addition to astaxanthin derived from natural products such as plants, algae, bacteria, and the like. Examples of the natural product include Phaffia rhodozyma, Haematococcus, marine bacteria, and krill. Further, extracts from the culture thereof, and the like can be also exemplified.

Preferably, the astaxanthin in the astaxanthin composition of the present invention is astaxanthin obtained by extracting Haematococcus sp as a raw material. Enumerable redThe Haematococcus includes Haematococcus pluvialis, Haematococcus lake, etc. Furthermore, the astaxanthin of the invention is produced by taking haematococcus pluvialis as a raw material and passing through CO₂The dark red oily extract obtained by supercritical extraction without solvent contains no less than 5.5% of total carotenoid, wherein the content of natural total astaxanthin is no less than 5%, and the content of all-trans astaxanthin is no less than 3.5%. The astaxanthin of the present invention has an antioxidant effect, an anti-inflammatory effect, an anti-aging effect on the skin, a whitening effect, and the like, and can impart an excellent emollient effect, an anti-aging effect on the skin, and an antioxidant effect.

The astaxanthin is added in an amount of 0.01-5%, preferably 0.05-5%, based on the total mass of the astaxanthin composition, for example: may be 0.03%, 0.06%, 0.08%, 0.1%, 0.12%, 0.2%, 0.5%, 0.8%, 1%, 1.2%, 1.5%, 1.8%, 2.0%, 2.2%, 2.5%, 2.8%, 3%, 3.2%, 3.5%, 3.8%, 4%, 4.2%, 4.5%, etc. When the addition amount of the astaxanthin is 0.01-5%, the astaxanthin can be compounded with the microalgae oil and the oat kernel oil for use, and the product has good illumination and temperature stability, is not easy to fade and keeps good antioxidant activity when being applied to cosmetic skin care products.

In some specific embodiments, the weight ratio of microalgae oil, oat kernel oil and astaxanthin of the present invention is 1 (0.9-2.8) to (0.0002-0.2); preferably 1 (1-2.3) to 0.0017-0.2, for example: 1 (1.1-2.2) (0.002-0.18), 1 (1.2-2.1) (0.004-0.15), 1 (1.3-2) (0.007-0.14), 1 (1.4-1.9) (0.01-0.12), 1 (1.5-1.8) (0.02-0.1), and 1 (1.6-1.7) (0.04-0.0.08). When the weight ratio of the microalgae oil to the oat kernel oil to the astaxanthin is 1 (0.9-2.8) to (0.0002-0.2), the effects of the components can be exerted most effectively, so that the astaxanthin composition can obtain more excellent antioxidant activity, light stability and thermal stability.

In addition, the invention also provides a preparation method of the astaxanthin composition, which comprises the step of mixing the components of the astaxanthin composition. Specifically, the preparation method comprises the following steps: mixing the microalgae oil, the oat kernel oil and the astaxanthin at normal temperature, and stirring at 100-1000rpm/min for 1-20min to obtain the astaxanthin composition.

In a second aspect the present invention provides an emulsification system comprising an astaxanthin composition according to the first aspect of the invention. By adding the astaxanthin composition into an emulsification system, the emulsification system has excellent antioxidant activity under high temperature and illumination conditions.

In some specific embodiments, the astaxanthin composition is added in an amount of 0.01 to 10% by mass, based on the total mass of the emulsifying system, for example: 0.5%, 1%, 2%, 3%, 4%, 5%, 6%, 7%, 8%, 9%, etc. The astaxanthin composition is used optimally when added in an amount of 0.01-10%. When the addition amount of the astaxanthin composition is less than 0.01%, the antioxidant activity cannot be exerted or the effect of the antioxidant activity is poor; when the addition amount of the astaxanthin composition is more than 10%, the content of the astaxanthin composition is too high, the cost is too high, the concentration of the astaxanthin composition in the skin care product is too high, and the astaxanthin composition is yellow and easy to color when being smeared on the skin.

Furthermore, the emulsifying system also comprises one or the combination of more than two of an emulsifier, grease, a thickening agent, a humectant and a preservative. Based on the total mass of the emulsifying system, the addition amount of the emulsifying agent is 0.1-5%; the addition amount of the grease is 0.1-40%; the addition amount of the thickening agent is 0.01-5%; the addition amount of the humectant is 0.01-20%; the addition amount of the preservative is 0.01-2%. The formula of the emulsifying system is mild, so that the efficacy of the astaxanthin composition can be fully exerted. Specifically, the method comprises the following steps:

in the present invention, the emulsifier is added in an amount of 0.01 to 5% based on the total mass of the emulsifying system, for example: may be 0.1-5%, may be 1-4%, may be 1-3%, etc., for example: 0.5%, 0.8%, 1.5%, 2%, 2.5%, 3.5%, 4.5%, etc. When the dosage of the emulsifier is less than 0.01%, the emulsification is insufficient, so that the system is unstable; when the dosage of the emulsifier is more than 5 percent, the skin discomfort can be caused, the adverse reactions such as acne and stimulation can be caused, and certain influence can be caused on the stability of the product.

In the present invention, the emulsifier may include one or a combination of two or more of cetearyl alcohol, cetearyl glucoside, PEG-100 stearate, polyglyceryl-10 stearate, glyceryl stearate/PEG-100 stearate, sorbitan sesquioleate, isosteareth-20, PEG-10 campesterol, laureth-7, polyglyceryl-3 methylglucose distearate, PEG/PPG-10/1 polydimethylsiloxane, polyglyceryl-3 diisostearate, glyceryl stearate, steareth-20, ceteth-20, and polysorbate-60.

In the invention, the addition amount of the grease is 0.1-40% by the total mass of the emulsifying system; for example, the amount of the oil or fat added may be 1%, 5%, 10%, 15%, 20%, 25%, 30%, 35%, or the like. By adding oil and fat into the emulsifying system, evaporation of water on the surface of the skin can be reduced, and skin chapping can be prevented. In addition, by adding oil or fat, a hydrophobic film can be formed on the skin surface to prevent the invasion of harmful substances from the outside. When the content of the oil is less than 0.1%, evaporation of moisture on the skin surface cannot be reduced, and intrusion of harmful substances cannot be effectively prevented; when the content of the oil is more than 40%, the emulsified system is too greasy, and the use feeling is reduced.

In the present invention, the oil and fat may include one or a combination of two or more of isopropyl myristate, polydimethylsiloxane, caprylic/capric triglyceride, dioctyl carbonate, hydrogenated polyisobutene, shea butter, ethylhexyl palmitate, hydrogenated polydecene, isononyl isononanoate, ethylhexyl cetyl, phytosterols, sunflower seed oil, olive oil unsaponifiable matter.

The addition amount of the thickening agent is 0.01-5% of the total mass of the emulsifying system. The consistency of the emulsifying system of the invention can be adapted by adding a thickener. Preferably, the thickener of the present invention may be added in an amount of 0.05 to 4%, 0.1 to 3%, 1 to 3%, or the like, for example: 0.02%, 0.08%, 0.3%, 0.5%, 0.8%, 1.5%, 2%, 2.5%, 3.5%, 4.5%, etc.

In the invention, the thickening agent comprises one or the combination of more than two of carbomer, carbomer salt, xanthan gum, sodium polyacrylate, carrageenan, gellan gum, hydroxyethyl cellulose, acryloyl dimethyl ammonium taurate/VP copolymer and hydroxyethyl acrylate/acryloyl dimethyl sodium taurate copolymer.

The addition amount of the humectant is 0.01-20% by total mass of the emulsifying system. When the addition amount of the humectant is 0.01-20%, the humectant can play a role in keeping moisture. In order to further exert the efficacy of the humectant, the humectant of the present invention may be added in an amount of 1 to 18%, 3 to 16%, 5 to 14%, 6 to 12%, for example: 2%, 3%, 6%, 8%, 10%, 12%, 15%, 17%, 19%, etc.

In the invention, the humectant comprises one or a combination of more than two of butanediol, panthenol, dipropylene glycol, glycerol, betaine, xylitol, sodium hyaluronate, trehalose, pentanediol, hexanediol and 1, 3-propanediol.

The emulsifying system of the invention can also contain a preservative, and the addition amount of the preservative is 0.01-2% of the total mass of the emulsifying system; specifically, the preservative may include one or a combination of two or more of phenoxyethanol, methylparaben, propylparaben, ethylhexylglycerin, benzoic acid and salts thereof.

In addition, the emulsifying system of the invention is not limited to the above components, but other ingredients that can be used in a possible cosmetic or dermatological product may also be added, such as: whitening additives, skin conditioners, soothing agents, pH adjusters, chelating agents, other antioxidants, and the like.

The invention also provides a preparation method of the emulsifying system, which comprises the step of mixing the components of the emulsifying system.

Specifically, the preparation method comprises the following steps:

mixing emulsifier and oil at 75-85 deg.C, and keeping moisture for 1-20min to obtain phase A;

mixing thickener, humectant and water, homogenizing for 1-5min, heating to 75-85 deg.C, maintaining the temperature for 5-30min, and stirring to dissolve completely to obtain phase B;

adding phase A into phase B, homogenizing for 1-10min, and stirring for 1-10 min;

cooling to 40-50 deg.C, and adding antiseptic;

finally adding the astaxanthin composition, uniformly mixing, cooling to 35-45 ℃, and discharging.

< third aspect >

A third aspect of the invention provides the use of an astaxanthin composition according to the invention or of an astaxanthin composition according to the invention in a skin care or cosmetic product. The astaxanthin composition is applied to skin care products or cosmetics, so that the skin care products or the cosmetics have the effect of antioxidant activity and can still have stable antioxidant activity under the conditions of high temperature or illumination.

Specifically, the skin care product or cosmetic can comprise one or more of emulsion, essence, sleep mask, cream, skin oil, massage cream, lipstick and lipstick.

Examples

Embodiments of the present invention will be described in detail below with reference to examples, but those skilled in the art will appreciate that the following examples are only illustrative of the present invention and should not be construed as limiting the scope of the present invention. The examples, in which specific conditions are not specified, were conducted under conventional conditions or conditions recommended by the manufacturer. The reagents or instruments used are not indicated by the manufacturer, and are all conventional products commercially available.

In the examples, the microalgae oil producer: lublorun, model, HSHO microalgal oil;

the astaxanthin manufacturer: cyanotech corporation, west noc, model number BioAstin;

the production factory of the oat kernel oil: finland (Fazer) food company.

Example 1

Taking 25 parts by weight (25%) of microalgae oil, 70 parts by weight (70%) of oat kernel oil and 5 parts by weight (5%) of astaxanthin, wherein the weight percentage of the microalgae oil, the oat kernel oil and the astaxanthin is 1:2.8: 0.2. Sequentially adding the components into a clean container, stirring at 500rpm/min for 10min, uniformly mixing to obtain an astaxanthin composition I, and hermetically storing at normal temperature for later use.

Example 2

30 parts by weight (30%) of microalgae oil, 69.99 parts by weight (69.99%) of oat kernel oil and 0.01 parts by weight (0.01%) of astaxanthin, wherein the weight percentages of microalgae oil, oat kernel oil and astaxanthin are about 1:2.33: 0.0003. And sequentially adding the components into a clean container, stirring at 300rpm/min for 2min, uniformly mixing to obtain an astaxanthin composition II, and hermetically storing at normal temperature for later use.

Example 3

38 parts by weight (38%) of microalgae oil, 60.5 parts by weight (60.5%) of oat kernel oil and 1.5 parts by weight (1.5%) of astaxanthin are taken, wherein the weight percentage of microalgae oil, oat kernel oil and astaxanthin is about 1:1.6: 0.039. Sequentially adding the components into a clean container, stirring at 400rpm/min for 4min, uniformly mixing to obtain the astaxanthin composition III, and hermetically storing at normal temperature for later use.

Example 4

Taking 50 parts by weight (50%) of microalgae oil, 45 parts by weight (45%) of oat kernel oil and 5 parts by weight (5%) of astaxanthin, wherein the weight percentage of the microalgae oil, the oat kernel oil and the astaxanthin is 1:0.9: 0.1. Sequentially adding the components into a clean container, stirring at 500rpm/min for 10min, uniformly mixing to obtain an astaxanthin composition IV, and hermetically storing at normal temperature for later use.

Example 5

42 parts by weight (42%) of microalgae oil, 54.8 parts by weight (54.8%) of oat kernel oil and 3.2 parts by weight (3.2%) of astaxanthin are taken, wherein the weight percentage of microalgae oil to oat kernel oil to astaxanthin is about 1:1.3: 0.076. Sequentially adding the components into a clean container, stirring at 350rpm/min for 5min, uniformly mixing to obtain the astaxanthin composition V, and hermetically storing at normal temperature for later use.

Example 6

Taking 40 parts by weight (40%) of microalgae oil, 58 parts by weight (58%) of oat kernel oil and 2 parts by weight (2%) of astaxanthin, wherein the weight percentage of the microalgae oil, the oat kernel oil and the astaxanthin is 1:1.45: 0.05. Sequentially adding the components into a clean container, stirring at 300rpm/min for 4.5min, uniformly mixing to obtain the astaxanthin composition VI, and hermetically storing at normal temperature for later use.

Comparative example 1 an astaxanthin composition VII was prepared by taking 15 parts by weight (about 16.67%) of microalgae oil, 70 parts by weight (about 77.78%) of oat kernel oil, and 5 parts by weight (about 5.55%) of astaxanthin, sequentially adding the components to a clean container, stirring at 500rpm/min for 10min, and mixing well, and was stored under sealed conditions at room temperature for future use.

Comparative example 2

Taking 38 parts by weight (about 58.91%) of microalgae oil, 25 parts by weight (about 38.76%) of oat kernel oil and 1.5 parts by weight (about 2.33%) of astaxanthin, sequentially adding the components into a clean container, stirring at 400rpm/min for 4min, uniformly mixing to obtain an astaxanthin composition VIII, and hermetically storing at normal temperature for later use.

Comparative example 3

Taking 50 parts by weight (about 47.62%) of microalgae oil, 45 parts by weight (about 42.86%) of oat kernel oil and 10 parts by weight (about 9.52%) of astaxanthin, sequentially adding the components into a clean container, stirring at 500rpm/min for 25min, uniformly mixing to obtain an astaxanthin composition IX, and hermetically storing at normal temperature for later use.

Comparative example 4

Taking 42 parts by weight (42%) of olive oil, 54.8 parts by weight (54.8%) of oat kernel oil and 3.2 parts by weight (3.2%) of astaxanthin, sequentially adding the components into a clean container, stirring at 350rpm/min for 5min, uniformly mixing to obtain an astaxanthin composition X, and hermetically storing at normal temperature for later use.

Comparative example 5

And (3) sequentially adding 96.8 parts by weight (96.8%) of tocopherol acetate and 3.2 parts by weight (3.2%) of astaxanthin into a clean container, stirring at 350rpm/min for 5min, uniformly mixing to obtain an astaxanthin composition XI, and hermetically storing at normal temperature for later use.

Application examples 1 to 6 and application comparative examples 1 to 5

The astaxanthin compositions I to VI of examples 1 to 6 were added to the emulsion formulation of Table 1 in a mass fraction of 1%, and the astaxanthin compositions VII to XI of comparative examples 1 to 5 were added to the emulsion formulation of Table 2 in a mass fraction of 1%, with the formulation without the addition of the astaxanthin composition as a control. 12 types of emulsion were prepared as follows.

The preparation steps of the emulsion are as follows:

sequentially adding polyglycerol-10 stearate, glycerol stearate/PEG-100 stearate, caprylic/capric triglyceride, polydimethylsiloxane and sunflower seed oil in the phase A into a clean oil phase pot, heating to 80 deg.C, and maintaining for 10 min;

sequentially adding deionized water, carbomer sodium, acryloyl dimethyl ammonium taurate/VP copolymer, 1, 3-propylene glycol, panthenol, trehalose and sodium hyaluronate in the phase B into a clean emulsifying pot, homogenizing at high speed for 3min, heating to 80 ℃, keeping the temperature for 15min, and fully stirring until the materials are completely dissolved;

pumping the phase A into an emulsifying pot, homogenizing at high speed for 4min, and stirring for 5 min;

cooling the cooling water to 45 ℃, and sequentially adding phenoxyethanol and ethylhexylglycerin in the phase C;

the astaxanthin composition in phase D was added last (control without this phase);

and (3) cooling to 38 ℃ after fully and uniformly stirring, discharging, filtering, and obtaining an emulsion sample after the emulsion is qualified by inspection.

TABLE 1 emulsion composition of application examples 1-6

TABLE 2 emulsion compositions using comparative examples 1-5 and control

Performance testing

1. Stability test

Samples of 11 types of emulsions containing astaxanthin prepared using the astaxanthin compositions I to VI of examples 1 to 6 and the astaxanthin compositions VII to XI of comparative examples 1 to 5 and emulsions of the control group without the astaxanthin composition were placed in transparent PET bottles and stored under 4 conditions, respectively, to be tested, and the color and odor changes of the samples under the respective conditions were observed, and the test results are shown in Table 3 below.

And (3) testing conditions are as follows:

the samples were allowed to stand at 25 ℃ and 60% RH at ambient temperature for comparative testing.

High-temperature acceleration: the test conditions were 50 ℃ plus or minus 1 ℃ and 25% RH plus or minus 5% RH for 1 month.

Xenon lamp illumination test: the illumination intensity is 0.35W/M²Test 26.4 hours, blackboard temperature 45 ℃ (non-box temperature), humidity 50%.

Outdoor light: standing for 10 days under natural light irradiation.

TABLE 3 stability test results for emulsions prepared using examples 1-6 and using comparative examples 1-5 and control

As can be seen from Table 3, the control emulsion containing no astaxanthin composition showed no change in appearance at high temperature due to the absence of any antioxidant, and an off-flavor was generated due to the accelerated oxidation of sunflower seed oil; meanwhile, oxidation of the sunflower seed oil is accelerated by a xenon lamp and outdoor illumination, so that the color of the emulsion is changed and peculiar smell is generated. The emulsions containing astaxanthin compositions I to VI prepared in application examples 1 to 6 did not undergo color and odor changes at room temperature, high temperature, xenon lamp and outdoor light.

Compared with the application example 1 and the application comparative example 1, the application comparative example 1 has less microalgae oil content and causes excessive oat kernel oil and astaxanthin content, which is not in the scope of the invention, so that the color of the emulsion of the astaxanthin composition VII is changed from red orange to light orange at high temperature, xenon lamp and outdoor illumination, namely the astaxanthin is mostly degraded.

Compared with the application example 3 and the application comparative example 2, the content of the oat kernel oil in the application comparative example 2 is less, so that the content of the microalgae oil is excessive, and the emulsion color and the smell of the astaxanthin-containing composition VIII are consistent with those of the emulsion at room temperature under the high-temperature condition, but the xenon lamp and the outdoor light color become lighter and orange compared with the room temperature, namely the astaxanthin is degraded to a certain degree.

Compared with application example 4 and application comparative example 3, the application comparative example 3 has high astaxanthin content, which results in that the content of the oat kernel oil is too low, which is not within the scope of the invention, and the emulsion of the astaxanthin-containing composition IX becomes light in color under each condition, but the odor is not changed, namely, the microalgae oil and the oat kernel oil in the concentration range can only protect part of the astaxanthin from degradation. Meanwhile, due to the high astaxanthin content, the mixing of the astaxanthin composition IX requires longer stirring time.

Application example 5 and application comparative example 4 compared, when the microalgae oil in application comparative example 4 was replaced with olive oil, the emulsion containing astaxanthin composition X was lightened in color at high temperature to protect a part of astaxanthin from degradation, but the color of xenon lamp and outdoor light was faded to completely degrade astaxanthin.

Application example 5 compared to application example 5, where the microalgal oil and oat kernel oil of application example 5 were replaced with tocopheryl acetate, the emulsion of astaxanthin-containing composition XI was degraded and discolored without odor generation under each condition, whereas the emulsion appeared yellowish in appearance due to the reaction of tocopheryl acetate under xenon lamp and light.

2. DPPH radical scavenging test- -antioxidant test

Relevant tests were carried out using application examples 1,3 to 4 and application comparative examples 1 to 3

DPPH is a stable free radical in an organic solvent, and an alcoholic solution of DPPH is purple, has a single electron, can capture other free radicals, and has the maximum absorption at the wavelength of 517 nm. In the presence of the radical scavenger, the single electron of DPPH is captured to lighten the color thereof, the light absorption value decreases at the maximum light absorption wavelength in a linear relationship, and the decrease in the light absorption level indicates an increase in the oxidation resistance, thereby evaluating the oxidation resistance of the test sample. The antioxidant ability is expressed by inhibition rate, and the greater the inhibition rate, the stronger the antioxidant ability.

Samples of the stability test in which the stability test using the I, III-IV emulsions containing astaxanthin prepared in examples 1,3 and 4, and the VI-IX emulsions containing astaxanthin prepared in comparative examples 1 to 3 and the control emulsion containing no astaxanthin were subjected to the test under three conditions of normal temperature, high temperature and outdoor light test were evaluated, and the DPPH radical scavenging effect was observed to evaluate the change in the oxidation resistance of astaxanthin.

Mixing 0.2mM DPPH alcoholic solution and prepared 30mg/mL aqueous solution containing astaxanthin composition and control group tested under each condition according to a ratio of 1:1, putting the mixture on a 96-well plate, reacting each group with 3 multiple wells in a water bath at 37 ℃ in a constant temperature and in a dark place for 30min, measuring the absorbance at 517nm, and calculating DPPH clearance of each sample, wherein the test result is shown in figure 1.

Clearance rate ═ 1- (A)_{Sample set}-A_{Blank group})/A_{Control group}]×100％

A_{Sample set}: light absorption values of the sample solution and the DPPH alcohol solution;

A_{blank group}: the light absorption values of the sample solution and the absolute ethyl alcohol;

A_{control group}: absorbance of alcoholic DPPH solution and water.

As can be seen from FIG. 1, the emulsion of the control sample without the astaxanthin composition, which does not contain any antioxidant, has a poor DPPH radical scavenging effect, i.e., the control sample has no antioxidant activity.

The emulsions containing the astaxanthin compositions I, III-VI prepared in application examples 1, 3-4 showed little change in DPPH radical scavenging rate after testing at room temperature, elevated temperature and outdoor light conditions, i.e., astaxanthin was able to maintain good antioxidant activity at elevated temperature and outdoor light conditions.

As shown in fig. 1, compared with application example 1 and application comparative example 1, the application comparative example 1 has a lower content of microalgae oil and leads to an excessive content of oat kernel oil and astaxanthin, which is not in the scope of the present invention, so that the DPPH radical clearance of the emulsion of the astaxanthin composition VII after high temperature and outdoor illumination is reduced, that is, the antioxidant activity of the astaxanthin is degraded is obviously reduced.

Compared with the application example 3 and the application comparative example 2, the content of the oat kernel oil in the application comparative example 2 is less, so that the content of the microalgae oil is excessive, the content is not in the range of the invention, the emulsion containing the astaxanthin composition VIII under the high-temperature condition and the DPPH free radical scavenging rate under the normal temperature are not obviously changed, but the astaxanthin is degraded to a certain degree when the outdoor illumination and the room temperature are relatively visible.

Compared with application example 4 and application comparative example 3, the application comparative example 3 has high astaxanthin content, so that the content of the oat kernel oil is too low, and the content is not in the range of the invention, and the emulsion containing the astaxanthin composition IX can keep a certain free radical scavenging effect under all conditions, namely the microalgae oil and the oat kernel oil in the concentration range can only protect part of the astaxanthin from degradation and keep part of the antioxidant activity.

The above examples of the present invention are merely examples for clearly illustrating the present invention and are not intended to limit the embodiments of the present invention. Other variations and modifications will be apparent to persons skilled in the art in light of the above description. And are neither required nor exhaustive of all embodiments. Any modification, equivalent replacement, and improvement made within the spirit and principle of the present invention should be included in the protection scope of the claims of the present invention.

Claims

1. An astaxanthin composition, which is characterized by comprising the following components in percentage by mass based on the total mass of the astaxanthin composition:

microalgae oil: 25 to 50 percent;

oat kernel oil: 45 to 70 percent;

astaxanthin: 0.01 to 5 percent.

2. The astaxanthin composition according to claim 1, wherein the microalgal oil is added in an amount of 30-50%, the oat kernel oil is added in an amount of 50-70%, and the astaxanthin is added in an amount of 0.05-5% by weight based on the total weight of the astaxanthin composition.

3. An astaxanthin composition according to claim 1 or 2, wherein the weight ratio of microalgal oil, oat kernel oil and astaxanthin is 1 (0.9-2.8): 0.0002-0.2); preferably 1 (1-2.3) and 0.0017-0.2.

4. A method of producing an astaxanthin composition according to any one of claims 1 to 3, comprising the step of mixing the components of the astaxanthin composition.

5. An emulsification system comprising an astaxanthin composition according to any one of claims 1 to 3; preferably, the astaxanthin composition is added in an amount of 0.01-10% by mass based on the total mass of the emulsifying system.

6. The emulsification system of claim 5, further comprising one or a combination of two or more of an emulsifier, a grease, a thickener, a humectant, and a preservative.

7. The emulsifying system according to claim 6, wherein the emulsifier is added in an amount of 0.1-5% based on the total mass of the emulsifying system; the addition amount of the grease is 0.1-40%; the addition amount of the thickening agent is 0.01-2%; the addition amount of the humectant is 0.01-20%; the addition amount of the preservative is 0.01-2%.

8. Method for preparing an emulsifying system according to any one of claims 4-7, characterized in that it comprises a step of mixing the components of the emulsifying system.

9. Use of an astaxanthin composition according to any one of claims 1 to 3 or an astaxanthin composition according to any one of claims 4 to 7 in skin care or cosmetic products.

10. The use according to claim 9, wherein the skin care or cosmetic product comprises one or a combination of two or more of a lotion, a serum, a sleep mask, a cream, a skin oil, a massage cream, a lipstick, and a lipstick.