CN113080195B - Termite trapping bait and using method thereof - Google Patents
Termite trapping bait and using method thereof Download PDFInfo
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- CN113080195B CN113080195B CN202110195970.1A CN202110195970A CN113080195B CN 113080195 B CN113080195 B CN 113080195B CN 202110195970 A CN202110195970 A CN 202110195970A CN 113080195 B CN113080195 B CN 113080195B
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/48—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with two nitrogen atoms as the only ring hetero atoms
- A01N43/56—1,2-Diazoles; Hydrogenated 1,2-diazoles
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
- A01N25/002—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests containing a foodstuff as carrier or diluent, i.e. baits
- A01N25/006—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests containing a foodstuff as carrier or diluent, i.e. baits insecticidal
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
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- Agronomy & Crop Science (AREA)
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- Insects & Arthropods (AREA)
- Food Science & Technology (AREA)
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- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
A soil termite trapping bait and a using method thereof belong to the technical field of termite control. The preparation method comprises the following steps: uniformly mixing 0.0005-0.008% of chlorantraniliprole and 99.9995-99.992% of grass and wood bait according to the mass ratio to prepare the trapping and killing bait. According to the soil-dwelling termite trapping and killing bait and the using method thereof, chlorantraniliprole and paper mulberry powder are adopted for matching, the paper mulberry powder attracts termites, and the chlorantraniliprole poisons the termites, so that the trapping and killing bait has a remarkable poisoning effect on the soil-dwelling termites, is short in prevention and control operation time and good in effect, the dosage of the chlorantraniliprole is obviously reduced, the environmental pollution is small, and the risk of drug resistance can be reduced.
Description
Technical Field
The invention belongs to the technical field of termite control, and particularly relates to a soil-dwelling termite trapping and killing bait and a using method thereof.
Background
Termites are social insects which take cellulose and lignin as food sources, and have acquired unique characteristics after the evolution of the termites for hundreds of millions of years: firstly, the termite bait has extremely strong adaptability and viability, and can live as long as the environment has certain temperature, humidity and foodstuff; secondly, the reproductive capacity of the termites is very strong, and the number of individuals in the termites is increased in a geometric progression; and thirdly, the medicine can be spread and diffused through the ways of separating flight, spreading invasion, carrying and the like. Two fifths of the area of China is damaged by termites, and the termite-proof board mainly relates to the fields of reservoir dams, river dikes, house buildings, historical relic and ancient buildings, power cables, decoration, landscaping, agricultural and forestry crops and the like, and causes great loss to national economy.
However, the termite control technology in China mainly controls chemical termites for a long time, and chemical medicines are applied in a large area, so that potential risks are brought to the ecological environment and the health of people while termite control is carried out. In order to change the prevention and control technology which is not environment-friendly, the applicant develops a monitoring and controlling device which is combined with the traditional powder spraying technology, namely the monitoring powder spraying technology, and the use amount of chemicals can be reduced by 99 percent by using the technology. In 2004, the international convention on stockholm reduction and elimination of organic pollutants formally takes effect in China, and the highly toxic pesticides are gradually eliminated and stopped in the industry of termite control, so that the development and application of high-efficiency, low-toxicity and low-residue pesticides are imperative. The termite trapping and killing bait agent receives more and more attention due to the advantages of good effect, little pollution, low cost and the like, and obtains some important research results. At present, the termite trapping bait has the problems of few varieties, unobvious effect and the like.
Disclosure of Invention
Aiming at the problems in the prior art, the invention aims to design and provide the technical scheme of the soil-dwelling termite trapping and killing bait agent and the using method thereof.
The soil-dwelling termite trapping bait is characterized by being prepared by the following steps: uniformly mixing 0.0005-0.008% of chlorantraniliprole and 99.9995-99.992% of grass and wood bait according to the mass ratio to prepare the trapping and killing bait.
The soil termite trapping and killing bait is characterized in that the plant bait is one or a mixture of more than one of paper mulberry powder, pine powder, wormwood powder, bark powder and aircraft grass powder.
The soil termite trapping and killing bait is characterized in that the grass and wood bait is 14-100 meshes, preferably 20-90 meshes, more preferably 30-80 meshes, and most preferably 40-60 meshes.
The soil termite trapping and killing bait is characterized in that 0.0005 percent of chlorantraniliprole and 99.9995 percent of plant bait are uniformly mixed to prepare the trapping and killing bait according to the mass ratio.
The soil-dwelling termite trapping and killing bait is characterized in that 0.005 percent of chlorantraniliprole and 99.995 percent of vegetation bait are uniformly mixed by mass ratio to prepare the trapping and killing bait.
The soil termite trapping and killing bait is characterized in that 0.008% of chlorantraniliprole and 99.992% of plant bait are uniformly mixed to prepare the trapping and killing bait.
The using method of the soil-dwelling termite trapping and killing bait is characterized by comprising the following steps of:
the field environment is used: throwing 1-5 bags of bait agent, preferably 2-3 bags, with the bait agent content of each bag being 8-12g according to 8-10 square meters in the first throwing and the row spacing and the spacing being 2-4m respectively, and throwing 1-5 bags and 2-3 bags in the subsequent throwing according to 20-30 square meters and the row spacing and the spacing being 4-6m respectively; checking for 1 time every 15 days after putting, supplementing in time after being eaten, or directly putting around mud quilt, mud line, ant path, flying hole or wood-eaten, covering with weeds or fallen leaves, and pressing a little soil;
use around gardens termite and housing construction: putting the bait agent around mud blankets, mud lines, ant tunnels or branch holes with termite activity, wherein each point is 1-5 bags, the bait agent content of each bag is 8-12g, covering the bag with weeds or fallen leaves, pressing a little soil, and trapping the underground termites to eat; checking for 1 time every 15 days after feeding, supplementing in time after being eaten, and killing the termites in the whole nest after 2-3 months.
The soil-dwelling termite trapping bait is characterized in that: uniformly mixing 0.0005-0.008% of chlorantraniliprole and 99.9995-99.992% of broussonetia papyrifera powder by mass ratio to prepare the trapping bait.
The chlorantraniliprole is a diamide pesticide, and has the action mode that: mainly takes stomach toxicity as a main component, has a certain contact action and has good systemic property and permeability. The chlorantraniliprole has high action speed and long lasting period, can effectively control almost all lepidoptera pests and part of other pests, and provides excellent crop protection effect by high-efficiency larvicidal activity and lasting effect. Chlorantraniliprole belongs to o-formamido benzamide insecticides, has a unique insecticidal mechanism, can efficiently activate an insect ryanodine (muscle) receptor, excessively releases calcium ions in cells, and leads insects to die by paralysis. Chlorantraniliprole can be used as an ideal termite trapping and killing bait, namely has the common characteristics of a termite control agent (small dosage, chronic property, good toxicity transmission and control effect), and can be used as a termite control drug.
The paper mulberry (Broussonetia papyrifera) is named as Broussonetia papyrifera and the like, the leaves of the plants in the family Moraceae of the order Urticales are good pig feed, the bast fibers of the plants are high-grade raw materials for papermaking, the materials are pure white, the roots and the seeds of the plants can be used as medicines, and the sap can treat skin diseases, so the economic value is high. The protein content of the broussonetia papyrifera leaves is up to 20-30%, and the broussonetia papyrifera leaves are also rich in nutrient components such as amino acids, vitamins, carbohydrates, trace elements and the like. The broussonetia papyrifera is used as a medicine by emulsion, root bark, leaves, fruits and seeds. Collecting the emulsion, leaves, fruits and seeds in summer and autumn; root bark and bark are collected in winter and spring and are used fresh or dried in the shade. Folium Broussonetiae has insecticidal effect, and can be used for treating neurodermatitis and tinea by external application on bark; the lotion has effects of inducing diuresis, relieving swelling and removing toxic substance, and can be used for treating snake, insect, bee, scorpion, and dog bite. The broussonetia papyrifera powder adopted by the invention is prepared by drying and crushing whole plants serving as raw materials.
According to the bait and the use method thereof, the broussonetia papyrifera powder is adopted to be matched with the chlorantraniliprole, the broussonetia papyrifera powder lures termites, and the chlorantraniliprole poisons the termites.
Detailed Description
The present invention will now be described more fully hereinafter with reference to the accompanying specific embodiments, in which some, but not all embodiments of the invention are shown. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
The first embodiment is as follows: uniformly mixing 0.0005% of chlorantraniliprole and 99.9995% of paper mulberry powder in mass ratio, and packaging with kraft paper bags to prepare the bait bag.
Example two: uniformly mixing 0.002% of chlorantraniliprole and 99.992% of paper mulberry powder in mass ratio, and packaging with kraft paper bags to prepare the trapping and killing bait bag.
Example three: uniformly mixing 0.005% of chlorantraniliprole and 99.995% of paper mulberry powder in mass ratio, and packaging with kraft paper bags to prepare the bait bag.
Example four: uniformly mixing 0.008% of chlorantraniliprole and 99.992% of paper mulberry powder in mass ratio, and packaging with kraft paper bags to prepare the trapping and killing bait bag.
Example five: uniformly mixing 0.0005% of chlorantraniliprole and 99.9995% of bark powder in mass ratio, and packaging with kraft paper bags to prepare the bait bag.
Example six: uniformly mixing 0.002% of chlorantraniliprole and 99.992% of aircraft grass powder in mass ratio, and packaging with kraft paper bags to prepare the trapping and killing bait bag.
Example seven: after uniformly mixing 0.005% of chlorantraniliprole and 99.995% of mixture of pine powder and paper mulberry powder in mass ratio, the ratio of the pine powder: the weight ratio of the paper mulberry powder is 1:2, and the paper mulberry powder is packaged by kraft paper bags to prepare the trapping and killing bait bag.
Example eight: uniformly mixing 0.008% of chlorantraniliprole and 99.992% of paper mulberry powder, pine powder and wormwood powder according to the mass ratio, and mixing the mixture of bark powder and organic grass powder uniformly, wherein the ratio of paper mulberry powder to pine powder is as follows: wormwood powder: according to bark powder: the weight ratio of the organic grass powder is 1:1:1.5:1:2, and the organic grass powder is packaged by kraft paper bags to prepare the trapping and killing bait bag.
The application method of the soil-dwelling termite trapping and killing bait comprises the following steps:
the field environment is used: throwing 1-5 bags of bait agent, preferably 2-3 bags, with the bait agent content of each bag being 8-12g according to 8-10 square meters in the first throwing and the row spacing and the spacing being 2-4m respectively, and throwing 1-5 bags and 2-3 bags in the subsequent throwing according to 20-30 square meters and the row spacing and the spacing being 4-6m respectively; checking for 1 time every 15 days after putting, supplementing in time after being eaten, or directly putting around mud quilt, mud line, ant path, flying hole or wood-eaten, covering with weeds or fallen leaves, and pressing a little soil;
use around gardens termite and house building: putting the bait agent around mud blankets, mud lines, ant tunnels or branch holes with termite activity, wherein each point is 1-5 bags, the bait agent content of each bag is 8-12g, covering the bag with weeds or fallen leaves, pressing a little soil, and trapping the underground termites to eat; checking for 1 time every 15 days after putting, supplementing in time after being eaten, and killing the termites in the whole nest after 2-3 months.
The technical effects of the present invention are further demonstrated by the corresponding test data below.
Test one: a layer of 5mm thick wet fine sand is paved in a culture dish with the diameter of 10cm, the termite trapping and killing bait and the control termite trapping and killing bait which do not contain chlorantraniliprole of the embodiments 1-4 are respectively placed in the culture dishes, 100 worker termites of black-wing soil termites and 15 soldier termites of black-wing soil termites are placed in each culture dish, the culture dishes are covered, and the culture dishes are placed in a constant temperature and humidity test box with the adjusted temperature and humidity (the temperature is 26-28 ℃, and the humidity is 75-85%) for feeding and observation. Distilled water was added dropwise periodically to wet. The number of Gordonian termites dead per 24 hours was recorded until the number of drug-treated Gordonian termites dead no longer changed for 3 consecutive days. Calculating the mortality rate after the test is finished, and calculating the corrected mortality rate when the control group ants die; the test should be restarted when the control worker ant mortality rate exceeds 10%. The experiment was repeated 3 times. The corrected mortality rate of the white-fin termite worker ants is shown in table 1.
The mortality rate = the number of dead workers/the number of trial workers x100%
Corrected mortality rate = (treatment group worker ant mortality rate-control group worker ant mortality rate)/(1-control group worker ant mortality rate) x100%
As can be seen from table 1, after eating the bait of examples 1 to 4, respectively, the respective corrected mortality rates of the black-fin odontotermes formosanus were: 19.04%, 46.49%, 59.87%, 66.88%; after 48 hours, the respective worker ant corrected mortality rates were: 60.47%, 80.94%, 89.79%, 91.83%; after 72 hours, the respective worker ant corrected mortality rates were: 86.64%, 99.30%, 100.00%, 100.00%; after 96h, the corresponding worker ants all had a corrected mortality rate of 100.00%. The result shows that the termite trapping bait containing 0.0005%, 0.002%, 0.005% and 0.008% of chlorantraniliprole has better poisoning effect on the odontotermes formosanus.
And (2) testing II:
taking four plants of paper mulberry, robinia pseudoacacia, paulownia and wormwood which are seriously damaged by termites in Luoyang city, drying and crushing the four plants to prepare 4 termite bait bags. Paving a layer of 5mm wet fine sand in a ceramic plate of 40x60cm, taking the 4 termite bait bags 1, respectively wrapping in a forced air drying oven at 50 ℃ for 24 hours, taking out and weighing, respectively putting into the ceramic plate, putting 500 black-wing odontotermes formosanus and 30 black-wing odontotermes warrior soldiers, covering the ceramic plate, and putting into a constant temperature and humidity test box with adjusted temperature and humidity (the temperature is 26-28 ℃ and the humidity is 75-85%) for feeding and observation. Distilled water was added dropwise periodically to wet. And taking out the bait bag after 120 hours, drying the bait bag in a blast drying oven at 50 ℃ for 24 hours, taking out the bait bag, weighing the bait bag, calculating the feeding rate, and repeating the test for 3 times. The intake rate is shown in table 2.
Intake rate = (mass before intake-mass after intake)/mass before intake x100%
TABLE 24 bait bags
| Name of Material | Average intake rate (%) |
| Paper mulberry powder | 21.40±1.36a |
| Robinia pseudoacacia powder | 1.19±0.31c |
| Paulownia powder | 2.37±1.35c |
| Artemisia apiacea powder | 18.14±2.69b |
Note: the data in the table are mean values + -SD, and the same horizontal row number is marked with different lower case letters to represent the result of' SPSS Duncan，The s "method differs significantly at the 5% level.
As can be seen from Table 2, the odontotermes formosanus has different degrees of feeding for 4 kinds of bait bags, the highest feeding rate is paper mulberry powder (21.40%), and the next time is tarragon powder (18.14%) which is far higher than paulownia powder (2.37%) and robinia pseudoacacia powder (1.19%), and the feeding rate of the paper mulberry powder is obviously different from that of the robinia pseudoacacia powder, the paulownia powder and the tarragon powder. The paper mulberry powder is suitable for being used as bait of the black wing odontotermes formosanus trapping bait.
Comparative example one: uniformly mixing 0.01% of chlorantraniliprole and 99.99% of broussonetia papyrifera powder by mass ratio, and packaging with a kraft paper bag to prepare the trapping and killing bait bag.
The test device adopts three PVC round cups with the inner diameter of 50mm and the height of 50mm, and the PVC round cups with the inner diameter of 6mm and the length of 50mm are connected with the PVC round cups at the position 5mm away from the bottom; before the test, fine sand which is 5mm thick and passes through a 250-micron sieve is respectively paved in three round cups of a test device, water is added for wetting, and then a glass sheet which is 30mm in diameter and 2mm in thickness is respectively placed between the surfaces of the fine sand in A, C two round cups; and (3) putting the trap-killing bait bag and the paper mulberry powder bag into an air drying oven at 50 ℃ for 24 hours, taking out, accurately weighing 5.00g of the trap-killing bait bag, placing the trap-killing bait bag in the middle of a glass plate in a round cup of a test device A, and accurately weighing 5.00g of the paper mulberry powder bag, placing the paper mulberry powder bag in the middle of a glass plate in a round cup of a test device C. Introducing 1000 healthy workers of black-wing odontotermes formosanus and 50 soldiers of black-wing odontotermes formosanus into a round cup of a test device B, covering A, B, C round cups of the test device with a gauze, then moving the test device into a constant-temperature constant-humidity test box (the temperature is 26-28 ℃ and the humidity is 75% -85%), taking out a bait agent trapping and killing bag and a paper mulberry powder bag after 120 hours, drying for 24 hours at 50 ℃ in a blast drying box, taking out and weighing, calculating the feeding rate, and repeating the test for 3 times. The intake rate is shown in Table 3.
Intake rate = (mass before intake-mass after intake)/mass before intake x100%
TABLE 3 bait intake rate
| Bait agent | Feed intake (%) |
| Bait bag for trapping and killing termites | 16.24±0.99a |
| Paper mulberry powder bag | 16.77±0.97a |
Note: the data in the table are mean values. + -. standard deviation, and the same horizontal row number is marked with different lower case letters to represent the result of "SPSS Duncan，The s "method differs significantly at the 5% level.
As can be seen from table 2: the feeding rates of the black wing soil termite bait bag and the broussonetia papyrifera powder bag prepared by uniformly mixing 0.01% of chlorantraniliprole and 99.99% of broussonetia papyrifera powder in mass ratio are not obviously different, which shows that the bait bag prepared by uniformly mixing 0.01% of chlorantraniliprole and 99.99% of broussonetia papyrifera powder in mass ratio has no avoidance effect on the black wing soil termite.
As can be seen from the above examples, the bait prepared by uniformly mixing 0.0005-0.008% of chlorantraniliprole and 99.9995-99.992% of broussonetia papyrifera powder in mass ratio has remarkable trapping and killing effects. The chlorantraniliprole with the mass ratio of 0.01 percent (and less) and the broussonetia papyrifera powder with the mass ratio of 99.99 percent are uniformly mixed to prepare the bait bag which has no evasion effect on the odontotermes formosanus.
According to the invention, different materials such as paper mulberry powder, pine powder, wormwood powder, bark powder, aircraft grass powder and the like can be selected according to different use regions and different termite species, and also several kinds of baits can be mixed. The beneficial effects of the invention can also be achieved by carrying out the same tests in the fifth embodiment, the sixth embodiment, the seventh embodiment and the eighth embodiment of the invention.
Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.
Claims (6)
1. A soil termite trapping bait is characterized by being prepared by the following steps: uniformly mixing 0.0005-0.008% of chlorantraniliprole and 99.9995-99.992% of grass and wood bait in a mass ratio to prepare a trapping bait agent; the plant bait is at least one of paper mulberry powder and wormwood powder.
2. The soil-dwelling termite bait formulation as set forth in claim 1, wherein the grass-wood bait is 14-100 mesh.
3. The soil-dwelling termite bait as claimed in claim 1, wherein the bait is prepared by uniformly mixing 0.0005% by mass of chlorantraniliprole and 99.9995% by mass of a plant bait.
4. The soil-dwelling termite bait as claimed in claim 1, wherein the bait is prepared by uniformly mixing 0.005% by mass of chlorantraniliprole and 99.995% by mass of a plant bait.
5. The soil-dwelling termite bait as claimed in claim 1, wherein 0.008% by mass of chlorantraniliprole and 99.992% by mass of the plant bait are uniformly mixed to prepare the bait.
6. The method of using a pesticidal bait for termites as claimed in claim 1, which comprises the steps of:
the field environment is used: the bait is put into a square meter according to 8-10 square meters for the first time, the row spacing and the space are respectively 2-4m, 1-5 bags of bait are put, the content of each bag of bait is 8-12g, the subsequent putting is carried out according to a square meter of 20-30 square meters, the row spacing and the space are respectively 4-6m, 1-5 bags are put, the check is carried out for 1 time every 15 days after the putting, the bait is supplemented in time after being eaten, or the bait is directly put into the mud quilt, the mud line, the ant path, the flying holes or the wood damaged by the moths, covered by weeds or fallen leaves, and then a little soil is pressed;
use around gardens termite and house building: putting the bait agent around mud blankets, mud lines, ant tunnels or branch holes with termite activity, wherein each point is 1-5 bags, the bait agent content of each bag is 8-12g, covering the bag with weeds or fallen leaves, pressing a little soil, and trapping the underground termites to eat; checking for 1 time every 15 days after putting, supplementing in time after being eaten, and killing the termites in the whole nest after 2-3 months.
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