CN113025467A

CN113025467A - Deoxyribonucleic acid extraction element among nucleic acid testing process

Info

Publication number: CN113025467A
Application number: CN202110404042.1A
Authority: CN
Inventors: 章贤骏; 宣兆康; 雷诺成; 白文鑫
Original assignee: Hangzhou Anyu Technologies Co ltd
Current assignee: Hangzhou Anyu Technologies Co ltd
Priority date: 2021-04-15
Filing date: 2021-04-15
Publication date: 2021-06-25
Anticipated expiration: 2041-04-15
Also published as: CN113025467B

Abstract

The invention discloses a deoxyribonucleic acid extraction device in a nucleic acid detection process, which comprises a deoxyribonucleic acid extraction instrument, an automatic pushing device and a sample pushing device, wherein a moving cavity is formed in the deoxyribonucleic acid extraction instrument, a moving frame for placing a sample is inserted in the moving cavity, the automatic pushing device for automatically moving the moving frame in the moving cavity is arranged on the inner wall of the moving cavity, and the sample pushing device for pushing the sample to a specified position in the deoxyribonucleic acid extraction instrument and performing anti-scald treatment after detection is finished is arranged in the moving frame.

Description

Deoxyribonucleic acid extraction element among nucleic acid testing process

Technical Field

The invention relates to the technical field of deoxyribonucleic acid extraction, in particular to a deoxyribonucleic acid extraction device in a nucleic acid detection process.

Background

Before the nucleic acid detection process, it is necessary to extract deoxyribonucleotides in cells and sufficiently stir the cell sap, so that the deoxyribonucleotides exist in the cell nucleus. After the cell sap is mixed with distilled water, the cell sap must be rapidly stirred along one direction by a glass rod to accelerate the cell rupture and release deoxyribonucleic acid, and then the extraction of the deoxyribonucleic acid can be completed by separating the deoxyribonucleic acid.

At present, relevant equipment is designed and operated on the market in order to avoid manual stirring extraction of deoxyribonucleic acid by people, but the relevant equipment on the market has the following defects: the sample is manually placed into or taken out of the detection module, the detection module has high temperature after analysis, an operator is scalded, the safety is not high, and if the operation is carried out after temperature reduction, the waiting time is too long, so that the deoxyribonucleic acid extraction device in the nucleic acid detection process is provided.

Disclosure of Invention

The present invention aims to provide a deoxyribonucleic acid extraction device in a nucleic acid detection process, which improves automation and can prevent people from being scalded during operation, so as to solve the problems in the background art.

In order to achieve the purpose, the invention provides the following technical scheme: the utility model provides a deoxyribonucleic acid extraction element among nucleic acid testing process, includes deoxyribonucleic acid extraction appearance, automatic pusher and sample pusher, it has the removal chamber to open in the deoxyribonucleic acid extraction appearance, and removes the intracavity and ann inserts the removal frame that is used for the sample to place for remove the frame and remove intracavity automatically move automatic pusher installs on removing the intracavity wall, is used for with sample propelling movement to the assigned position in the deoxyribonucleic acid extraction appearance and carry out the processing of preventing scalding after the detection finishes sample pusher installs in removing the frame.

Preferably, automatic pushing device is including fixing driving motor and two threaded rods on removing the intracavity wall, arbitrary threaded rod one end is fixed with the driving motor output, and another threaded rod passes through the bearing and removes frame inner wall rotation and be connected, two the equal screw thread of threaded rod outer end has cup jointed a screw thread section of thick bamboo, and two screw thread sections all slide and run through and remove the frame tail end to it is fixed with removal frame front end inner wall, two the threaded rod outside all is fixed with the link gear, and two link gear outside meshing has cup jointed first toothed belt, realizes removing the frame and remove at the removal intracavity through automatic pushing device, realizes the automatic feed ejection of compact.

Preferably, sample pusher is including fixing regulating plate and two lead screws in removing the frame both sides, it all is fixed with the propelling movement motor to remove frame tail end inner wall, and propelling movement motor output end passes through the shaft coupling and fixes with arbitrary lead screw one end, and another lead screw one end is passed through the bearing and is connected with removing the rotation of frame inner wall, and two lead screw other ends all rotate through bearing and removal frame front end inner wall and be connected, and two lead screw outsides all are fixed with drive gear, and two drive gear outside meshing has cup jointed the second toothed belt, two the centre gripping subassembly is installed in the lead screw outside, and installs on removing the frame bottom inner wall and prevent scalding the piece, removes the frame removal to the sample through sample pusher realizes.

Preferably, the clamping assembly comprises two U-shaped frames, L-shaped plates are fixed on the outer sides of the two U-shaped frames, the L-shaped plates are in threaded sleeve connection with the screw rod and are in sliding sleeve connection with the outer side of the threaded cylinder, a moving opening is formed in each U-shaped frame, an adjusting table is inserted in the moving opening in a sliding manner, an adjusting rod is inserted in the adjusting table in a sliding manner, the outer end of the adjusting rod penetrates through the U-shaped frames in a sliding manner and is fixed with a trapezoidal table, a clamping member for clamping and limiting a sample is fixed between the two adjusting tables, track grooves are formed in the adjacent sides of the two adjusting plates, a convex block matched with the track grooves in shape is fixed on the adjacent side of each adjusting plate, a sliding head in sliding connection with the track grooves is fixed on the outer side of each adjusting table, a connecting column matched with the convex block is fixed on the outer side of each trapezoidal table, an adjusting opening is formed in the, the clamping of the sample cup is realized through the clamping assembly, and the device is convenient to move and operate.

Preferably, the clamping part comprises positioning plates with arc-shaped openings at two ends, connecting rods fixed with the adjusting table outside are fixed at two ends of each positioning plate, movable plates with the arc-shaped openings are arranged on the outer sides of two ends of each connecting rod in a sliding manner, two adjusting arms are installed on the surfaces of the movable plates in a rotating manner, the outer ends of the adjusting arms are connected with the bottoms of two ends of the trapezoidal table in a rotating manner, extrusion springs are sleeved at two ends of each connecting rod between the positioning plates and the corresponding movable plate, sample cups are arranged between the positioning plates and the movable plates, and clamping of the two sample cups is achieved through the clamping part.

Preferably, prevent scalding the piece and include two prevent scalding the ring and be located the removal track of preventing scalding the ring outside, two prevent scalding the ring both ends and all be fixed with the connecting block of removal track looks adaptation, and remove frame bottom and open have with prevent scalding the small opening of ring looks adaptation, prevent scalding the ring through preventing scalding the piece and make personnel when taking in the cover of sample cup outside, can not scald the hand.

Preferably, the opening is internally provided with a chamfer matched with the trapezoidal table, so that the trapezoidal table can move more smoothly when moving to a delicious place.

Compared with the prior art, the invention has the beneficial effects that:

according to the automatic pushing device and the sample pushing device, the automatic pushing and taking out of the sample are realized through mutual matching of the automatic pushing device and the sample pushing device, so that a person is prevented from being scalded by high temperature in the device when taking the sample, and the use safety is improved.

Drawings

FIG. 1 is a schematic view of the overall structure of the present invention;

FIG. 2 is a partial cross-sectional view of the internal structure of the present invention;

FIG. 3 is a schematic view of the connection between the automatic pushing device and the moving frame according to the present invention;

FIG. 4 is a schematic cross-sectional view of the moving frame according to the present invention;

FIG. 5 is a schematic structural diagram of a sample pushing apparatus according to the present invention;

FIG. 6 is a schematic view, partially in section, of the inventive clamping assembly;

FIG. 7 is a schematic view of a partial cross-sectional view of a gripping member of the present invention;

FIG. 8 is an enlarged view taken at A in FIG. 4;

fig. 9 is an enlarged view of fig. 5 at B.

In the figure: 1-deoxynucleic acid extractor; 2-a moving cavity; 3-moving the frame; 4-an automatic pushing device; 5-a sample pushing device; 6-driving a motor; 7-a threaded rod; 8-a threaded cylinder; 9-a linkage gear; 10-a first toothed belt; 11-an adjusting plate; 12-a screw rod; 13-a push motor; 14-a transmission gear; 15-a second toothed belt; 16-a clamping assembly; 17-scald prevention parts; 18-U shaped frame; 19-L type board; 20-a moving port; 21-an adjusting table; 22-adjusting rod; 23-a trapezoidal table; 24-clamping; 25-a track groove; 26-a bump; 27-a sliding head; 28-connecting column; 29-an adjustment port; 30-opening; 31-a positioning plate; 32-connecting rod; 33-moving the plate; 34-an adjusting arm; 35-a compression spring; 36-sample cup; 37-scald preventing ring; 38-a moving track; 39-connecting block; 40-leakage hole.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

Referring to fig. 1-9, a deoxyribonucleic acid extraction device in a nucleic acid detection process shown in the figure includes a deoxyribonucleic acid extractor 1, an automatic pushing device 4 and a sample pushing device 5, a moving cavity 2 is formed in the deoxyribonucleic acid extractor 1, a moving frame 3 for placing a sample is inserted into the moving cavity 2, the automatic pushing device 4 for automatically moving the moving frame 3 in the moving cavity 2 is mounted on the inner wall of the moving cavity 2, and the sample pushing device 5 for pushing the sample to a specified position in the deoxyribonucleic acid extractor 1 and performing anti-scald treatment after detection is completed is mounted in the moving frame 3.

The automatic pushing device 4 comprises a driving motor 6 and two threaded rods 7, wherein the driving motor 6 and the two threaded rods 7 are fixed on the inner wall of the moving cavity 2, one end of any one threaded rod 7 is fixed with the output end of the driving motor 6, the other threaded rod 7 is rotatably connected with the inner wall of the moving frame 3 through a bearing, the outer ends of the two threaded rods 7 are respectively in threaded sleeve joint with a threaded cylinder 8, the two threaded cylinders 8 respectively penetrate through the tail end of the moving frame 3 in a sliding mode and are fixed with the inner wall of the front end of the moving frame 3, linkage gears 9 are fixed on the outer sides of the two threaded rods 7, and a first toothed belt 10 is in meshing;

principle of automatic pushing of the mobile frame 3: through the rotation of driving motor 6, drive threaded rod 7 and rotate, simultaneously under linkage gear 9 and the mutually supporting of first toothed belt 10 for two threaded rods 7 rotate in step, and then the propelling movement frame 3 fixed with a screw thread section of thick bamboo 8 carries out the back-and-forth movement in removing chamber 2, realizes the automatically regulated to removing frame 3.

In addition, the sample pushing device 5 comprises an adjusting plate 11 and two screw rods 12 fixed on both sides of the moving frame 3, the pushing motors 13 are fixed on the inner walls of the tail ends of the moving frame 3, the output end of a pushing motor 13 is fixed with one end of any screw rod 12 through a coupler, one end of the other screw rod 12 is rotationally connected with the inner wall of the movable frame 3 through a bearing, the other ends of the two screw rods 12 are rotationally connected with the inner wall of the front end of the movable frame 3 through bearings, a transmission gear 14 is fixed on the outer side of each of the two screw rods 12, and the outer sides of the two transmission gears 14 are engaged and sleeved with a second toothed belt 15, the outer sides of the two screw rods 12 are provided with clamping components 16, the inner wall of the bottom of the movable frame 3 is provided with an anti-scald part 17, by the rotation of the pushing motor 13 and the mutual cooperation of the transmission gear 14 and the second toothed belt 15, thereby enabling the two lead screws 12 to synchronously rotate and facilitating the position adjustment of the clamping assembly 16.

Wherein, the clamping assembly 16 comprises two U-shaped frames 18, L-shaped plates 19 are fixed on the outer sides of the two U-shaped frames 18, the L-shaped plates 19 are in threaded sleeve connection with the screw rod 12 and are in sliding sleeve connection with the outer side of the threaded cylinder 8, a moving port 20 is formed in each U-shaped frame 18, an adjusting table 21 is inserted in each moving port 20 in a sliding manner, an adjusting rod 22 is inserted in each adjusting table 21 in a sliding manner, the outer end of each adjusting rod 22 penetrates through the U-shaped frame 18 in a sliding manner and is fixed with a trapezoidal table 23, a clamping part 24 for limiting sample clamping is fixed between the two adjusting tables 21, track grooves 25 are formed in the adjacent sides of the two adjusting plates 11, convex blocks 26 with the shapes matched with the track grooves 25 are fixed on the adjacent sides of the adjusting plates 11, sliding heads 27 in sliding connection with the track grooves 25 are fixed on the outer sides of the adjusting tables 21, connecting columns 28 matched with the convex blocks 26 are fixed on the outer sides of the trapezoidal tables 23, the convex blocks, openings 30 are formed in the tops of two ends of the movable frame 3, wherein in order to enable the trapezoidal table 23 to move more smoothly in the openings 30, chamfers matched with the trapezoidal table 23 are formed on the inner side of each opening 30;

the clamping piece 24 comprises a positioning plate 31 with arc-shaped openings at two ends, connecting rods 32 fixed to the outer side of the adjusting table 21 are fixed to two ends of the positioning plate 31, moving plates 33 with arc-shaped openings at the outer sides are sleeved at two ends of each connecting rod 32 in a sliding mode, two adjusting arms 34 are rotatably mounted on the surfaces of the moving plates 33, the outer ends of the two adjusting arms 34 are rotatably connected with the bottoms of two ends of the trapezoidal table 23 through rotating shafts, extrusion springs 35 are sleeved between two ends of each connecting rod 32, which are located between the positioning plate 31 and the corresponding moving plate 33, sample cups 36 are arranged between the positioning plate 31 and the corresponding moving plate 33, and the sample cups 36 are clamped through extrusion of the positioning plate 31 and the corresponding moving plates 33, so that the samples in the sample cups;

simultaneously, prevent scalding piece 17 and include two prevent scalding ring 37 and be located the removal track 38 of preventing scalding the ring 37 outside, two prevent scalding ring 37 both ends all be fixed with the connecting block 39 who removes track 38 looks adaptation, and remove 3 bottoms of frame and open the small opening 40 that has and prevent scalding ring 37 looks adaptation.

Principle of taking out the sample cup 36: firstly, a person detects a sample in a sample cup 36 through the deoxyribonucleic acid extractor 1, after the detection is finished, the moving cavity 2 is pushed to the moving part of a moving frame 3 through an automatic pushing device 4, then the two L-shaped plates 19 move on a screw rod 12 through the rotation of a pushing motor 13 to drive a U-shaped frame 18 to move, in the moving process, the sample cup 36 touches an anti-scalding ring 37, the pushing anti-scalding ring 37 drops into a leak hole 40 along a moving track 38, meanwhile, the whole clamping part 24 is lifted up through the movement of a sliding head 27 in a track groove 25, the trapezoidal table 23 continues to move after moving to an opening 30 until a connecting column 28 moves to an adjusting opening 29, at the moment, the adjusting arm 34 is driven to push outwards along with the extrusion spring 35, the trapezoidal table 23 moves upwards along the adjusting opening 29, and meanwhile, the gap between the moving plate 33 and a positioning plate 31 is increased, so that the sample cup 36 falls into the anti-scald ring 37, and the person takes out the sample cup 36 by holding the anti-scald ring 37 to play the anti-scald function;

principle of insertion of the sample cup 36: the personnel rotate through lead screw 12 earlier, drive spliced pole 28 and move out from regulation mouth 29 for moving plate 33 and locating plate 31 dock, and the personnel are putting into sample cup 36 between, and rotate through lead screw 12, make sample cup 36 remove to remove frame 3 tail end, later will prevent scalding ring 37 and put on moving track 38 again, and through automatic pusher 4, will remove frame 3 propelling movement and move in the chamber 2, accomplish putting into to sample cup 36.

In the scheme, the type Y80M1-2 is preferably selected as the driving motor 6, the type Y80M2-2 is preferably selected as the pushing motor 13, a power supply interface of the driving motor is connected with a power supply system through a switch, a motor operation circuit is a normal and reverse rotation control program of a conventional motor, the circuit operates as a conventional circuit, circuits and control related to the scheme are the prior art, and redundant description is not repeated herein.

It is noted that, herein, relational terms such as first and second, and the like may be used solely to distinguish one entity or action from another entity or action without necessarily requiring or implying any actual such relationship or order between such entities or actions. Also, the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or apparatus.

Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.

Claims

1. A deoxyribonucleic acid extraction device in a nucleic acid detection process is characterized by comprising:

the device comprises a deoxyribonucleic acid extractor (1), wherein a moving cavity (2) is formed in the deoxyribonucleic acid extractor (1), and a moving frame (3) for placing a sample is inserted into the moving cavity (2);

the automatic pushing device (4) is used for installing the automatic pushing device (4) of the moving frame (3) in the moving cavity (2) to move automatically on the inner wall of the moving cavity (2);

the sample pushing device (5) is used for pushing a sample to a specified position in the deoxyribonucleic acid extractor (1) and carrying out scald-proof treatment after detection is finished, and the sample pushing device (5) is arranged in the movable frame (3).

2. The apparatus for extracting deoxyribonucleic acid (DNA) in a nucleic acid testing process according to claim 1, wherein: automatic pusher (4) is including fixing driving motor (6) and two threaded rods (7) on removing chamber (2) inner wall, arbitrary threaded rod (7) one end is fixed with driving motor (6) output, and another threaded rod (7) rotate with removal frame (3) inner wall through the bearing and be connected, two the equal screw thread in threaded rod (7) outer end has cup jointed a thread section of thick bamboo (8), and two thread section of thick bamboo (8) all slide and run through and remove frame (3) tail end to it is fixed with removal frame (3) front end inner wall, two threaded rod (7) outside all is fixed with linked gear (9), and two linked gear (9) outside meshing cup joints first profile of tooth area (10).

3. The apparatus for extracting deoxyribonucleic acid (DNA) in a nucleic acid testing process according to claim 2, wherein: sample pusher (5) is including fixing regulating plate (11) and two lead screw (12) in removal frame (3) both sides, it all is fixed with propelling movement motor (13) to remove frame (3) tail end inner wall, and propelling movement motor (13) output passes through the shaft coupling and fixes with arbitrary lead screw (12) one end, and another lead screw (12) one end is passed through the bearing and is connected with removal frame (3) inner wall rotation, and two lead screw (12) other ends all rotate with removal frame (3) front end inner wall through the bearing and are connected, and two lead screw (12) outsides all are fixed with drive gear (14), and two drive gear (14) outside meshing cover has connect second profile of tooth area (15), two centre gripping subassembly (16) are installed in lead screw (12) outside, and remove and install on frame (3) bottom inner wall and prevent scalding a (17).

4. The apparatus for extracting deoxyribonucleic acid (DNA) in a nucleic acid testing process according to claim 3, wherein: the clamping assembly (16) comprises two U-shaped frames (18), L-shaped plates (19) are fixed on the outer sides of the two U-shaped frames (18), the L-shaped plates (19) are in threaded sleeve connection with the screw rod (12) and are in sliding sleeve connection with the outer side of the threaded barrel (8), a moving opening (20) is formed in each U-shaped frame (18), an adjusting table (21) is inserted in each moving opening (20) in a sliding mode, an adjusting rod (22) is inserted in each adjusting table (21) in a sliding mode, the outer end of each adjusting rod (22) penetrates through the corresponding U-shaped frame (18) in a sliding mode and is fixed with a trapezoidal table (23), clamping pieces (24) used for clamping and limiting samples are fixed between the two adjusting tables (21), track grooves (25) are formed in the adjacent sides of the two adjusting plates (11), convex blocks (26) matched with the track grooves (25) in shape are fixed on the adjacent sides of the adjusting plates (11), and sliding heads (27) connected with the track grooves (25) in a sliding mode are fixed on, and the outer side of the trapezoidal table (23) is fixed with a connecting column (28) matched with the lug (26), the lug (26) is positioned at the front end of the movable frame (3) and is provided with an adjusting opening (29), and the tops of the two ends of the movable frame (3) are provided with openings (30).

5. The apparatus for extracting deoxyribonucleic acid (DNA) in a nucleic acid testing process according to claim 4, wherein: piece (24) are got including both ends locating plate (31) that are equipped with the arc mouth, locating plate (31) both ends all are fixed with connecting rod (32) fixed with regulation platform (21) outside, the equal sliding sleeve in connecting rod (32) both ends has movable plate (33) that the outside is equipped with the arc mouth, and movable plate (33) surface all rotates and installs two regulating arms (34), two regulating arm (34) outer end all rotates through pivot and trapezoidal platform (23) both ends bottom and is connected, connecting rod (32) both ends are located all to overlap between locating plate (31) and movable plate (33) and have extrusion spring (35), and are equipped with sample cup (36) between locating plate (31) and movable plate (33).

6. The apparatus for extracting deoxyribonucleic acid (DNA) in a nucleic acid testing process according to claim 5, wherein: prevent scalding piece (17) and include two prevent scalding ring (37) and be located prevent scalding removal track (38) in the ring (37) outside, two prevent scalding ring (37) both ends all are fixed with connecting block (39) with removal track (38) looks adaptation, and remove frame (3) bottom and open leak (40) that have with prevent scalding ring (37) looks adaptation.

7. The apparatus for extracting deoxyribonucleic acid (DNA) in a nucleic acid testing process according to claim 6, wherein: the inner side of the opening (30) is provided with a chamfer matched with the trapezoidal table (23).