CN112760218A - Automatic mesenchymal stem cell separation device - Google Patents
Automatic mesenchymal stem cell separation device Download PDFInfo
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- CN112760218A CN112760218A CN202110071315.5A CN202110071315A CN112760218A CN 112760218 A CN112760218 A CN 112760218A CN 202110071315 A CN202110071315 A CN 202110071315A CN 112760218 A CN112760218 A CN 112760218A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
- C12M47/04—Cell isolation or sorting
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D33/00—Filters with filtering elements which move during the filtering operation
- B01D33/01—Filters with filtering elements which move during the filtering operation with translationally moving filtering elements, e.g. pistons
- B01D33/015—Filters with filtering elements which move during the filtering operation with translationally moving filtering elements, e.g. pistons with flat filtering elements
- B01D33/0158—Filters with filtering elements which move during the filtering operation with translationally moving filtering elements, e.g. pistons with flat filtering elements self-supporting
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M21/00—Bioreactors or fermenters specially adapted for specific uses
- C12M21/18—Apparatus specially designed for the use of free, immobilized or carrier-bound enzymes
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
- C12M27/02—Stirrer or mobile mixing elements
- C12M27/04—Stirrer or mobile mixing elements with introduction of gas through the stirrer or mixing element
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
- C12M33/14—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus with filters, sieves or membranes
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
- C12M41/18—Heat exchange systems, e.g. heat jackets or outer envelopes
- C12M41/24—Heat exchange systems, e.g. heat jackets or outer envelopes inside the vessel
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/42—Means for regulation, monitoring, measurement or control, e.g. flow regulation of agitation speed
Abstract
The invention discloses an automatic mesenchymal stem cell separation device, which belongs to the technical field of cell advanced separation and comprises a workbench, and separation equipment, blanking equipment and screening equipment which are arranged in the workbench, wherein the separation equipment is arranged at the top of the workbench, the separation equipment is used for placing stem cell stock solution, enzyme and water and carrying out stirring and quick-drying cell separation, the blanking equipment is arranged in the workbench and positioned at the bottom of the separation equipment, the blanking equipment is used for assisting the separation equipment to carry out blanking and conveying stem cells after a separation procedure to the screening equipment, the screening equipment is arranged in the workbench and positioned at the bottom of the blanking equipment, and the screening equipment is used for screening the separated stem cells into mixed solution.
Description
Technical Field
The invention relates to the technical field of cell extraction and separation, in particular to an automatic mesenchymal stem cell separation device.
Background
Stem cells are a type of pluripotent cells with self-replication ability, which can be differentiated into various functional cells under certain conditions, and are classified into embryonic stem cells and adult stem cells according to the development stage of the stem cells, and are classified into three types according to the development potential of the stem cells: totipotent, pluripotent and unipotent stem cells; the stem cell is an insufficiently differentiated and immature cell, has potential functions of regenerating various tissues, organs and human bodies, and is called as a universal cell in the medical field; the stem cells are often mixed with other cells after being extracted, so a stem cell separation device is needed, and the separation step comprises the steps of firstly injecting stem cell stock solution, water and enzyme into a reaction container for reaction, and then collecting extract after the reaction is completed to complete the separation step.
Most of the existing mesenchymal stem cell separation and extraction processes are manually operated, the automation degree is low, the separation step is to inject stem cell stock solution, water and enzyme into a reaction container for reaction, and collect extract after the reaction is completed to complete the separation step, the process steps are complicated, the randomness is high, incomplete separation is easily caused by manual misoperation, and time and labor are consumed.
Disclosure of Invention
The invention provides an automatic mesenchymal stem cell separation device, which aims to solve the technical problems that in the prior art, the steps for separating mesenchymal stem cells are complicated, the randomness is high, the separation is incomplete easily caused by manual operation errors, and the time and the labor are consumed.
The utility model provides an automatic type mesenchymal stem cell separator, its characterized in that, includes the workstation and sets up splitter, unloading equipment and the screening equipment in the workstation, splitter sets up the top of workstation, the unloading equipment sets up the workstation is inside and is in splitter's bottom, the screening equipment sets up the workstation is inside and is in unloading equipment bottom.
Furthermore, the separation equipment comprises a separation support, a separation kettle, a transmission shaft, a transmission sleeve, a first gear, a first transmission gear and two separation rods, the separation support is arranged at the top of the workbench, a fixed shaft sleeve is arranged at the bottom of the separation support, the separation kettle is arranged at the bottom of the separation support and at the top of the workbench, an accommodating cavity with an upward opening is arranged on the separation kettle, the separation kettle is of a double-layer structure, a heating pipe is arranged inside the separation kettle, the transmission shaft is arranged in the fixed shaft sleeve and is coaxial with the separation kettle, the transmission shaft is rotatably connected with the fixed shaft sleeve, the two separation rods are respectively arranged at two ends of the transmission shaft, the two separation rods are rotatably connected with the transmission shaft, each separation rod is S-shaped, and the first gear is provided with two separation rods, two first gear sets up respectively two the top of release lever, transmission sleeve cover is established the fixed axle is sheathe in, just transmission sleeve with fixed axle sleeve sliding connection, first transmission gear sets up transmission sleeve's bottom, just first transmission gear respectively with two first gear engagement.
Further, the upside of first drive gear still is equipped with second drive gear, second drive gear's diameter is greater than the diameter of first drive gear, every the top of first gear all is equipped with the second gear, the diameter of second gear is less than the diameter of first gear, just second drive gear respectively with two the second gear meshing, separation support top still is equipped with the separation cylinder, the main shaft of separation cylinder runs through the separation support with the transmission sleeve joint.
Furthermore, a separation motor is arranged at the top of the separation support, a first bevel gear is arranged on a spindle of the separation motor, the top of the transmission shaft penetrates through the separation support, a second bevel gear is arranged at the top of the transmission shaft, and the first bevel gear is meshed with the second bevel gear.
Further, the blanking device comprises a blanking gear, a blanking motor, a fluted disc, a blanking pipe, five connecting rods and five chucks, a blanking port is arranged at the bottom of the separation kettle, the blanking pipe is arranged at the bottom of the blanking port, the blanking pipe is butted with the blanking port, a through groove is arranged at the circle center of the fluted disc, the fluted disc is arranged at the lower side of the blanking pipe, the five connecting rods are uniformly arranged at the bottom of the fluted disc according to a certain angle, and five first connecting columns are arranged on the fluted disc, five connecting rods are sleeved on the five first connecting columns, each connecting rod can rotate through the first connecting columns, five chucks are arranged at the bottom of the blanking pipe, and the blanking pipe is provided with five second connecting columns, five chucks are respectively sleeved on the five second connecting columns, and each chuck can rotate through the second connecting column, and the other ends of the five connecting rods are respectively hinged with the five chucks.
Further, screening equipment includes the screening board, accepts board and backup pad, the backup pad sets up workstation inboard bottom, just the backup pad with workstation fixed connection, it sets up to accept the board the backup pad top, be equipped with uncovered ascending chamber that holds in the backup pad, accept the board with backup pad fixed connection, the screening board sets up accept the board top, just it is equipped with the spout to accept the board top, screening board bottom is equipped with the gyro wheel, just the gyro wheel is in the spout simultaneously, the screening board accessible the gyro wheel is accepting the board and is slided from side to side, be equipped with uncovered ascending chamber that holds in the screening board, just the uncovered output towards unloading equipment that holds the chamber, just the chamber bottom that holds of screening board is equipped with the stem cell filter screen.
Further, screening equipment still includes screening motor, first connecting rod and second connecting rod, screening board bottom still is equipped with the transmission piece, the transmission piece with backup pad sliding connection, the one end of second connecting rod with the transmission piece is articulated, the other end of second connecting rod with first connecting rod is articulated, the screening motor sets up workstation inboard bottom, the main shaft of screening motor with first connecting rod fixed connection.
Furthermore, a storage plate is further arranged in the bearing plate, the storage plate extends leftwards to penetrate through the bearing plate, the storage plate is movably connected with the bearing plate, and a handle is arranged on the left side of the storage plate.
Compared with the prior art, the invention has the beneficial effects that:
firstly, when the separation motor works, the transmission shaft can be driven to rotate through the meshing action of the first bevel gear and the second bevel gear, the two separation rods can be driven to rotate at the moment, the first transmission gear is fixed, when the two separation rods rotate, the two separation rods rotate through the meshing action of the two first gears and the first transmission gear, the separation efficiency is improved, and when the rotating shaft rotates, the heating pipes in the separation kettle work simultaneously, so that the optimal separation temperature of the stem cell stock solution is maintained.
Secondly, when the transmission cylinder works, the transmission sleeve is driven to move upwards, the second transmission gear is in contact with the two second gears, and the speed of stirring and separating can be adjusted by adopting different direct gears.
Thirdly, when the blanking device works, the stem cell mixed liquid is released from the screening plate, the screening motor works at the moment, the screening plate is driven to slide on the bearing plate through the first connecting rod and the second connecting rod, the stem cell mixed liquid is screened through the stem cell filter screen and falls into the bearing plate, and stem cells are screened from the stem cell mixed liquid.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, and it is obvious that the drawings in the following description are some embodiments of the present invention, and other drawings can be obtained by those skilled in the art without creative efforts.
FIG. 1 is a first perspective view of the present invention;
FIG. 2 is a schematic perspective view of the present invention;
FIG. 3 is a schematic diagram of an exploded structure of the separation apparatus of the present invention;
FIG. 4 is an enlarged view taken at A in FIG. 3;
FIG. 5 is a schematic perspective view of the blanking apparatus of the present invention;
fig. 6 is a schematic view of an explosive structure of the blanking apparatus of the present invention;
figure 7 is a schematic illustration of the explosive structure of the screening apparatus of the present invention.
Reference numerals
The device comprises a workbench 1, a separating device 2, a blanking device 3, a screening device 4, a separating bracket 21, a separating kettle 22, a transmission shaft 23, a transmission sleeve 24, a first gear 25, a first transmission gear 26, a separating rod 27, a heating pipe 28, a second transmission gear 29, a second gear 210, a separating motor 211, a first bevel gear 212, a second bevel gear 213, a blanking gear 31, a blanking motor 32, a fluted disc 33, a blanking pipe 34, a connecting rod 35, a chuck 36, a first connecting column 37, a second connecting column 38, a screening plate 41, a bearing plate 42, a supporting plate 43, a roller 44, a chute 45, a screening motor 46, a first connecting rod 47, a second connecting rod 48, a storage plate 49 and a handle 410.
Detailed Description
The technical solutions of the present invention will be described clearly and completely with reference to the accompanying drawings, and it should be understood that the described embodiments are some, but not all embodiments of the present invention.
The components of embodiments of the present invention generally described and illustrated in the figures herein may be arranged and designed in a wide variety of different configurations. Thus, the following detailed description of the embodiments of the present invention, presented in the figures, is not intended to limit the scope of the invention, as claimed, but is merely representative of selected embodiments of the invention.
All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
In the description of the present invention, it should be noted that the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", etc., indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, and are only for convenience of description and simplicity of description, but do not indicate or imply that the device or element being referred to must have a particular orientation, be constructed and operated in a particular orientation, and thus, should not be construed as limiting the present invention. Furthermore, the terms "first," "second," and "third" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance.
In the description of the present invention, it should be noted that, unless otherwise explicitly specified or limited, the terms "mounted," "connected," and "connected" are to be construed broadly, e.g., as meaning either a fixed connection, a removable connection, or an integral connection; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meanings of the above terms in the present invention can be understood in specific cases to those skilled in the art.
As described below with reference to fig. 1 to 7, an embodiment of the present invention provides an automatic mesenchymal stem cell separation apparatus, including a work bench 1, and a separation device 2, a blanking device 3 and a sieving device 4 disposed in the work bench 1, the separation device 2 is arranged on the top of the workbench 1, the separation device 2 is used for placing stem cell stock solution, enzyme and water, and the separation of the stirred and accelerated fast cells is carried out, the blanking device 3 is arranged in the workbench 1 and at the bottom of the separation device 2, the blanking device 3 is used for assisting the separation device 2 in blanking, and the stem cells after the separation process are conveyed to a screening device 4, the screening device 4 is arranged in the workbench 1 and at the bottom of the blanking device 3, and the screening device 4 is used for screening the separated stem cells to obtain a mixed solution.
The separation device 2 comprises a separation support 21, a separation kettle 22, a transmission shaft 23, a transmission sleeve 24, a first gear 25, a first transmission gear 26 and two separation rods 27, wherein the separation support 21 is arranged at the top of the workbench 1, a fixed shaft sleeve is arranged at the bottom of the separation support 21, the separation kettle 22 is arranged at the bottom of the separation support 21 and is positioned at the top of the workbench 1, an upward-opened accommodating cavity is arranged on the separation kettle 22, the separation kettle 22 is of a double-layer structure, a heating pipe 28 is arranged inside the separation kettle 22, the heating pipe 28 is used for maintaining the optimal mixing temperature when mixing the stock solution of the dry cells, the transmission shaft 23 is arranged in the fixed shaft sleeve and is coaxial with the separation kettle 22, the transmission shaft 23 is rotatably connected with the fixed shaft sleeve, and the two separation rods 27 are respectively arranged at two ends of the transmission shaft 23, and two of the separation rods 27 are rotatably connected with the transmission shaft 23, each separation rod 27 is S-shaped, two first gears 25 are provided, the two first gears 25 are respectively arranged at the top of the two separation rods 27, the transmission sleeve 24 is sleeved on the fixed shaft sleeve, the transmission sleeve 24 is slidably connected with the fixed shaft sleeve, the first transmission gear 26 is arranged at the bottom of the transmission sleeve 24, the first transmission gear 26 is respectively meshed with the two first gears 25, when the transmission shaft 23 rotates, the two separation rods 27 can be driven to rotate, because the first transmission gear 26 is stationary, when the two separation rods 27 rotate, the two separation rods rotate by the meshing action of the two first gears and the first transmission gear 26, the separation efficiency is increased, when the rotation shaft rotates, the heating pipes 28 in the separation kettle 22 work simultaneously, maintaining the optimal separation temperature of the stem cell stock solution.
The upside of first drive gear 26 still is equipped with second drive gear 29, the diameter of second drive gear 29 is greater than the diameter of first drive gear 26, every the top of first gear 25 all is equipped with second gear 210, the diameter of second gear 210 is less than first gear 25's diameter, just second drive gear 29 respectively with two the meshing of second gear 210, separation support 21 top still is equipped with the separation cylinder, the main shaft of separation cylinder runs through separation support 21 with transmission sleeve 24 joint, works as transmission cylinder during operation, drives transmission sleeve 24 moves up, this moment second drive gear 29 and two the contact of second gear 210 is convenient for adjust the separation speed.
The top of the separation bracket 21 is provided with a separation motor 211, a main shaft of the separation motor 211 is provided with a first bevel gear 212, the top of the transmission shaft 23 penetrates through the separation bracket 21, the top of the transmission shaft 23 is provided with a second bevel gear 213, the first bevel gear 212 is meshed with the second bevel gear 213, and when the separation motor 211 works, the transmission shaft 23 can be driven to rotate through the meshing action of the first bevel gear 212 and the second bevel gear 213.
The blanking device 3 comprises a blanking gear 31, a blanking motor 32, a fluted disc 33, a blanking pipe 34, five connecting rods 35 and five chucks 36, wherein a blanking opening is arranged at the bottom of the separation kettle 22, the blanking pipe 34 is arranged at the bottom of the blanking opening, the blanking pipe 34 is in butt joint with the blanking opening, a through groove is formed in the circle center of the fluted disc 33, the fluted disc 33 is arranged at the lower side of the blanking pipe 34, the five connecting rods 35 are uniformly arranged at the bottom of the fluted disc 33 according to a certain angle, five first connecting columns 37 are arranged on the fluted disc 33, the five connecting rods 35 are sleeved on the five first connecting columns 37, each connecting rod 35 can rotate through the first connecting column 37, the five chucks 36 are arranged at the bottom of the blanking pipe 34, five second connecting columns 38 are arranged on the blanking pipe 34, the five chucks 36 are respectively sleeved on the five second connecting columns 38, and each chuck 36 can rotate through the second connecting columns 38, the other ends of the five connecting rods 35 are respectively hinged with the five chucks 36, when the fluted disc 33 is rotated, the five connecting rods 35 are firstly driven to rotate, because the blanking pipe 34 is fixed, at this time, the five chucks 36 are in transmission connection with the fluted disc 33 through the five connecting rods 35, five chucks 36 can be driven by five connecting rods 35 to rotate through five second connecting posts 38, respectively, the blanking motor 32 is arranged at the right side of the top of the workbench 1, and the main shaft of the blanking motor 32 penetrates through the workbench 1, the blanking gear 31 is fixedly connected to the main shaft of the blanking motor 32, and the blanking gear 31 is meshed with the fluted disc 33, when the blanking motor 32 works, the fluted disc 33 can be driven to rotate by the meshing action of the blanking gear 31 and the fluted disc 33 so as to control the opening and closing of the blanking pipe 34 to realize the blanking of the stem cell mixed liquid.
The screening device 4 comprises a screening plate 41, a receiving plate 42 and a supporting plate 43, the supporting plate 43 is arranged at the bottom of the inner side of the workbench 1, the supporting plate 43 is fixedly connected with the workbench 1, the receiving plate 42 is arranged at the top of the supporting plate 43, an accommodating cavity with an upward opening is arranged in the supporting plate 43, the receiving plate 42 is fixedly connected with the supporting plate 43, the screening plate 41 is arranged at the top of the receiving plate 42, a chute 45 is arranged at the top of the receiving plate 42, a roller 44 is arranged at the bottom of the screening plate 41, the roller 44 is simultaneously positioned in the chute 45, the screening plate 41 can slide left and right on the receiving plate 42 through the roller 44, an accommodating cavity with an upward opening is arranged in the screening plate 41, the opening of the accommodating cavity faces the output end of the blanking device 3, and a dry cell filter screen is arranged at the bottom of the accommodating cavity of the screening plate 41, when the blanking device 3 works, the stem cell mixed liquid is released to the sieving plate 41, and at the moment, the sieving plate 41 slides left and right on the receiving plate 42, so that the stem cell mixed liquid is sieved by the stem cell filter screen and falls into the receiving plate 42.
The receiving plate 42 is further provided with a storage plate 49, the storage plate 49 extends through the receiving plate 42 leftwards, the storage plate 49 is movably connected with the receiving plate 42, and a handle 410 is arranged on the left side of the storage plate 49, so that an operator can take the storage plate 49 out of the receiving plate 42 through the handle 410, and the screened stem cells are convenient to take.
Finally, it should be noted that: the above embodiments are only used to illustrate the technical solution of the present invention, and not to limit the same; while the invention has been described in detail and with reference to the foregoing embodiments, it will be understood by those skilled in the art that: the technical solutions described in the foregoing embodiments may still be modified, or some or all of the technical features may be equivalently replaced; and the modifications or the substitutions do not make the essence of the corresponding technical solutions depart from the scope of the technical solutions of the embodiments of the present invention.
Claims (8)
1. The utility model provides an automatic type mesenchymal stem cell separator, its characterized in that, includes workstation (1) and splitter (2), unloading equipment (3) and screening equipment (4) of setting in workstation (1), splitter (2) set up the top of workstation (1), unloading equipment (3) set up workstation (1) is inside and be in the bottom of splitter (2), screening equipment (4) set up workstation (1) is inside and be in unloading equipment (3) bottom.
2. The automatic mesenchymal stem cell separation device according to claim 1, wherein the separation apparatus (2) comprises a separation support (21), a separation kettle (22), a transmission shaft (23), a transmission sleeve (24), a first gear (25), a first transmission gear (26) and two separation rods (27), the separation support (21) is arranged at the top of the workbench (1), a fixed shaft sleeve is arranged at the bottom of the separation support (21), the separation kettle (22) is arranged at the bottom of the separation support (21) and at the top of the workbench (1), an accommodating cavity with an upward opening is arranged on the separation kettle (22), the separation kettle (22) has a double-layer structure, a heating pipe (28) is arranged inside the separation kettle (22), the transmission shaft (23) is arranged in the fixed shaft sleeve and is coaxial with the separation kettle (22), transmission shaft (23) with fixed axle sleeve rotates to be connected, two separation rod (27) sets up respectively the both ends of transmission shaft (23), and two separation rod (27) with transmission shaft (23) rotate to be connected, every separation rod (27) are the S type, first gear (25) are equipped with two, two first gear (25) set up respectively two the top of separation rod (27), transmission sleeve (24) cover is established the fixed axle is sheathe in, just transmission sleeve (24) with fixed axle sleeve sliding connection, first transmission gear (26) set up the bottom of transmission sleeve (24), just first transmission gear (26) respectively with two first gear (25) meshing.
3. The automatic mesenchymal stem cell separation device according to claim 2, wherein a second transmission gear (29) is further disposed on the upper side of the first transmission gear (26), the diameter of the second transmission gear (29) is greater than that of the first transmission gear (26), a second gear (210) is disposed on the top of each first gear (25), the diameter of the second gear (210) is smaller than that of the first gear (25), the second transmission gears (29) are respectively engaged with the two second gears (210), a separation cylinder is further disposed on the top of the separation bracket (21), and a main shaft of the separation cylinder penetrates through the separation bracket (21) and is clamped with the transmission sleeve (24).
4. The automatic mesenchymal stem cell separation device according to claim 2, wherein a separation motor (211) is disposed on the top of the separation bracket (21), a first bevel gear (212) is disposed on a main shaft of the separation motor (211), the top of the transmission shaft (23) penetrates through the separation bracket (21), a second bevel gear (213) is disposed on the top of the transmission shaft (23), and the first bevel gear (212) is engaged with the second bevel gear (213).
5. The automatic mesenchymal stem cell separation device according to claim 2, wherein the feeding device (3) comprises a feeding gear (31), a feeding motor (32), a fluted disc (33), a feeding pipe (34), five connecting rods (35) and five chucks (36), the bottom of the separation kettle (22) is provided with a feeding opening, the feeding pipe (34) is arranged at the bottom of the feeding opening, the feeding pipe (34) is butted with the feeding opening, a through groove is arranged at the center of the fluted disc (33), the fluted disc (33) is arranged at the lower side of the feeding pipe (34), the five connecting rods (35) are uniformly arranged at the bottom of the fluted disc (33) according to a certain angle, the fluted disc (33) is provided with five first connecting columns (37), the five connecting rods (35) are sleeved on the five first connecting columns (37), and each connecting rod (35) can rotate through the first connecting column (37), five chuck (36) set up in the bottom of unloading pipe (34), and are equipped with five second spliced poles (38) on unloading pipe (34), and five chuck (36) are established respectively on five second spliced poles (38), and every chuck (36) all can be rotatory through second spliced pole (38), and the other end of five connecting rods (35) is articulated with five chuck (36) respectively.
6. The automatic mesenchymal stem cell separation device according to claim 1, wherein the sieving device (4) comprises a sieving plate (41), a receiving plate (42) and a supporting plate (43), the supporting plate (43) is disposed at the inner bottom of the workbench (1), the supporting plate (43) is fixedly connected with the workbench (1), the receiving plate (42) is disposed at the top of the supporting plate (43), an open upward accommodating cavity is disposed in the supporting plate (43), the receiving plate (42) is fixedly connected with the supporting plate (43), the sieving plate (41) is disposed at the top of the receiving plate (42), a chute (45) is disposed at the top of the receiving plate (42), a roller (44) is disposed at the bottom of the sieving plate (41), and the roller (44) is simultaneously disposed in the chute (45), the sieving plate (41) can slide left and right on the receiving plate (42) through the roller (44), be equipped with uncovered ascending chamber that holds in screening board (41), just the uncovered output towards unloading equipment (3) that holds the chamber, just the chamber bottom that holds of screening board (41) is equipped with the stem cell filter screen.
7. The automatic mesenchymal stem cell separation device according to claim 6, wherein the sieving device (4) further comprises a sieving motor (46), a first connecting rod (47) and a second connecting rod (48), a transmission block is further arranged at the bottom of the sieving plate (41), the transmission block is slidably connected with the supporting plate (43), one end of the second connecting rod (48) is hinged with the transmission block, the other end of the second connecting rod (48) is hinged with the first connecting rod (47), the sieving motor (46) is arranged at the inner bottom of the workbench (1), and a main shaft of the sieving motor (46) is fixedly connected with the first connecting rod (47).
8. The device for separating mesenchymal stem cells according to claim 6, wherein the receiving plate (42) further comprises a storage plate (49), the storage plate (49) extends through the receiving plate (42) to the left, the storage plate (49) is movably connected to the receiving plate (42), and a handle (410) is disposed at the left side of the storage plate (49).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110071315.5A CN112760218A (en) | 2021-01-19 | 2021-01-19 | Automatic mesenchymal stem cell separation device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110071315.5A CN112760218A (en) | 2021-01-19 | 2021-01-19 | Automatic mesenchymal stem cell separation device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN112760218A true CN112760218A (en) | 2021-05-07 |
Family
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
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| CN202110071315.5A Withdrawn CN112760218A (en) | 2021-01-19 | 2021-01-19 | Automatic mesenchymal stem cell separation device |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114277641A (en) * | 2021-06-07 | 2022-04-05 | 广西交建工程检测咨询有限公司 | Bag heating system is torn open to pitch intelligence in bags |
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2021
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114277641A (en) * | 2021-06-07 | 2022-04-05 | 广西交建工程检测咨询有限公司 | Bag heating system is torn open to pitch intelligence in bags |
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Application publication date: 20210507 |