CN111979094A - Nucleic acid detection device - Google Patents
Nucleic acid detection device Download PDFInfo
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- CN111979094A CN111979094A CN202010886454.9A CN202010886454A CN111979094A CN 111979094 A CN111979094 A CN 111979094A CN 202010886454 A CN202010886454 A CN 202010886454A CN 111979094 A CN111979094 A CN 111979094A
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- 238000001514 detection method Methods 0.000 title claims abstract description 77
- 150000007523 nucleic acids Chemical class 0.000 title claims abstract description 61
- 102000039446 nucleic acids Human genes 0.000 title claims abstract description 61
- 108020004707 nucleic acids Proteins 0.000 title claims abstract description 61
- 230000001678 irradiating effect Effects 0.000 claims abstract description 7
- 230000007246 mechanism Effects 0.000 claims description 58
- 238000010438 heat treatment Methods 0.000 claims description 20
- 239000011248 coating agent Substances 0.000 claims description 8
- 238000000576 coating method Methods 0.000 claims description 8
- 230000000903 blocking effect Effects 0.000 claims description 7
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- 238000000034 method Methods 0.000 abstract description 13
- 239000007795 chemical reaction product Substances 0.000 abstract description 7
- 230000035945 sensitivity Effects 0.000 abstract description 4
- 230000008569 process Effects 0.000 description 9
- 238000002347 injection Methods 0.000 description 5
- 239000007924 injection Substances 0.000 description 5
- 229920006324 polyoxymethylene Polymers 0.000 description 4
- 239000003638 chemical reducing agent Substances 0.000 description 3
- 239000004677 Nylon Substances 0.000 description 2
- 230000004308 accommodation Effects 0.000 description 2
- XAGFODPZIPBFFR-UHFFFAOYSA-N aluminium Chemical group [Al] XAGFODPZIPBFFR-UHFFFAOYSA-N 0.000 description 2
- 229910052782 aluminium Inorganic materials 0.000 description 2
- 230000008901 benefit Effects 0.000 description 2
- 238000009833 condensation Methods 0.000 description 2
- 230000005494 condensation Effects 0.000 description 2
- 230000003760 hair shine Effects 0.000 description 2
- 239000012535 impurity Substances 0.000 description 2
- 230000031700 light absorption Effects 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 229920001778 nylon Polymers 0.000 description 2
- 229920000642 polymer Polymers 0.000 description 2
- 230000002265 prevention Effects 0.000 description 2
- 239000000243 solution Substances 0.000 description 2
- 230000007306 turnover Effects 0.000 description 2
- 208000035473 Communicable disease Diseases 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 230000002349 favourable effect Effects 0.000 description 1
- 238000007689 inspection Methods 0.000 description 1
- 230000003287 optical effect Effects 0.000 description 1
- TWNQGVIAIRXVLR-UHFFFAOYSA-N oxo(oxoalumanyloxy)alumane Chemical compound O=[Al]O[Al]=O TWNQGVIAIRXVLR-UHFFFAOYSA-N 0.000 description 1
- 244000052769 pathogen Species 0.000 description 1
- 230000001717 pathogenic effect Effects 0.000 description 1
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- 238000005070 sampling Methods 0.000 description 1
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- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
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- C12Q1/686—Polymerase chain reaction [PCR]
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Abstract
The present invention provides a nucleic acid detection device, including: a rack provided with a sample placing position; the image collector is arranged on the rack and is positioned above the sample placing position; the light source assembly is arranged on the rack and is positioned above the sample placing position; the light source assembly is used for irradiating a light source only used for exciting fluorescence; the light shielding part at least winds the periphery of the light emitting surface of the light source component and the periphery of the lens of the image collector by one circle, and the bottom of the light shielding part at least partially extends to the periphery of the sample placing position. According to the technical scheme, the sample placing position can be used for placing a certain amount of samples on a certain scale, so that the detection time of the conventional nucleic acid detection method can be greatly shortened on the premise of ensuring the sensitivity, and the method is suitable for application scenes of large-scale nucleic acid detection; meanwhile, the arrangement of the light shielding part can reduce the noise of the fluorescent signal, improve the accuracy of the amount of the PCR reaction product reflected by the fluorescent signal and improve the detection accuracy of the nucleic acid detection device.
Description
Technical Field
The invention relates to the technical field of medical detection, in particular to a nucleic acid detection device.
Background
With the outbreak of global epidemic situation, the increase of overseas input cases puts forward new requirements on the epidemic situation prevention and control situation of our country. Meanwhile, asymptomatic infectors increase the difficulty of epidemic prevention and control. Efficient and reliable pathogen detection methods and devices are important tools for the control and management of infectious diseases.
Conventional nucleic acid detection requires standard PCR laboratories, by specialized nucleic acid testers, through multi-step instrumentation and manual operations. In the prior art, for example, chinese patent document CN210215385U discloses a nucleic acid detecting apparatus, which includes a temperature-controlled stage, an image pickup lens disposed above the stage, and a light source. Be equipped with the apron on the top of objective table, enclose into the chamber that holds of sample between apron and the objective table, set up a plurality of inspection holes on the apron. The camera lens and the light source are both arranged above the cover plate; the light source is arranged on the periphery of the camera lens through the mounting frame; the top of the camera lens is provided with a moving device which is used for driving the camera lens to move in the directions of an X axis, a Y axis and a Z axis so as to change the positions of the camera lens and the light source.
The camera lens and the light source move under the driving of the transfer device to align to each detection hole, the light source irradiates the sample to excite the fluorescent chemical substances added in the sample, the camera lens collects images, and a fluorescence intensity signal curve is obtained through subsequent processing, and the higher the fluorescence signal intensity is, the more the PCR reaction products are accumulated. At present, in order to reduce the noise of the collected fluorescence intensity signal, an optical filter is usually disposed at the light exit surface of the light source, so that the light source irradiating on the sample is only light capable of exciting fluorescence, and other impurity light is filtered.
Although the light filter is disposed on the light emitting surface of the light source to filter out the impurity light, the periphery of the light source is in an external environment, the light source irradiates the path of the detection hole, and the light in the external environment is doped in the light source and irradiates the sample in the detection hole, so that the obtained fluorescence intensity signal contains noise, and the amount of the product in the PCR reaction cannot be reflected more accurately. That is, the background noise light content during the collection of the fluorescence signal is high, which affects the accuracy of the fluorescence intensity signal reflecting the amount of the PCR reaction product, and finally results in low detection accuracy of the nucleic acid detection device.
Therefore, there is a need for an improved nucleic acid detecting apparatus.
Disclosure of Invention
Therefore, the technical problem to be solved by the present invention is that in the fluorescent signal collection process of the nucleic acid detection device in the prior art, the background noise light content is high, which affects the accuracy of the amount of the PCR reaction product reflected by the fluorescent signal, resulting in low detection accuracy of the nucleic acid detection device.
To this end, the present invention provides a nucleic acid detecting apparatus comprising:
a rack provided with a sample placing position;
the image collector is arranged on the rack and is positioned above the sample placing position;
the light source assembly is arranged on the rack and is positioned above the sample placing position; the light source assembly is used for irradiating a light source only used for exciting fluorescence;
the light shielding part at least winds the periphery of the light emitting surface of the light source component and the periphery of the lens of the image collector by one circle, and the bottom of the light shielding part at least partially extends to the periphery of the sample placing position.
Furthermore, the shading part is a cover body with an opening at the bottom, and the top of the cover body is covered above the top of the image collector and the top of the light source component.
Furthermore, the shading part is arranged on the rack in a vertically slidable manner through a lifting mechanism.
Furthermore, the lifting mechanism comprises a lifting component fixed on the shading component and a first driving component which drives the lifting component to do lifting motion and is arranged on the rack.
Furthermore, at least one layer of light absorption layer is arranged on the outer wall surface and/or the inner wall surface of the light shielding component.
Further, the nucleic acid detecting apparatus further comprises a sample cartridge provided at the sample placement site; a sample is arranged on the sample placement site by being arranged in the sample cartridge; the top of the sample box is provided with detection ports which are in one-to-one correspondence with samples in the sample box;
the bottom of the light shielding part is at least partially wound on the periphery of the sample box.
Further, the sample cartridge includes:
a lower cover plate having an accommodation cavity;
the upper cover plate is movably arranged on the top opening of the lower cover plate under the driving of the first driver, and when the upper cover plate is buckled on the lower cover plate, an accommodating cavity is formed by the upper cover plate and the lower cover plate; the upper cover plate is provided with the detection port.
Furthermore, at least one heating element is respectively arranged on the inner wall surfaces of the upper cover plate and the lower cover plate; the temperature detector and the controller are electrically connected with the temperature detector and the heating element; and/or at least one layer of heat-resistant coating is arranged on the outer wall surface of the upper cover plate and/or the lower cover plate.
Furthermore, at least one heat conducting plate is arranged on the surface of one side of the heating element, which faces away from the cover plate on which the heating element is arranged; or at least one placing groove is arranged in the containing cavity of the lower cover plate, and the placing groove is used for embedding the PCR plate.
Further, the shading part is sleeved on the periphery of the rack; a sample inlet communicated with the sample placing position is formed in the side wall of the shading part;
the nucleic acid detection device also comprises a sample feeding mechanism, and the sample box is arranged on the sample feeding mechanism and driven by the sample feeding mechanism to slide along a first direction to extend into or withdraw from the sample placing position through the sample feeding port; and sliding at the sample placement location in a second direction perpendicular to the first direction.
Further, the sampling mechanism includes:
the first sliding mechanism is arranged on the rack and linearly slides in a reciprocating manner along a first direction;
the second sliding mechanism is arranged on the first sliding mechanism and directly slides in a reciprocating manner along a second direction;
a carrier arranged on a second slider of the second sliding mechanism; the sample cartridge is mounted on a top surface of the stage.
The technical scheme of the invention has the following advantages:
1. the present invention provides a nucleic acid detecting apparatus including:
a rack provided with a sample placing position;
the image collector is arranged on the rack and is positioned above the sample placing position;
the light source assembly is arranged on the rack and is positioned above the sample placing position; the light source assembly is used for irradiating a light source only used for exciting fluorescence;
the light shielding part at least winds the periphery of the light emitting surface of the light source component and the periphery of the lens of the image collector by one circle, and the bottom of the light shielding part at least partially extends to the periphery of the sample placing position. Through the arrangement of the technical scheme, the sample placing position can be used for placing a certain amount of samples in a certain scale, high-throughput sample detection is easy to realize, the detection time of the conventional nucleic acid detection method can be greatly shortened on the premise of ensuring the sensitivity, and the method is suitable for the application scene of large-scale nucleic acid detection; meanwhile, the arrangement of the light shielding part can reduce the noise of the fluorescent signal, improve the accuracy of the amount of the PCR reaction product reflected by the fluorescent signal and improve the detection accuracy of the nucleic acid detection device.
2. According to the nucleic acid detection device provided by the invention, the light shielding part is a cover body with an opening at the bottom, and the top of the cover body is covered above the image collector and the top of the light source component. Through the arrangement of the technical scheme, the light shielding part can shield the light emitted by the light source component and the image collector, so that the noise of the fluorescent signal is reduced.
3. According to the nucleic acid detection device provided by the invention, the light shielding part is arranged on the rack in a vertically slidable manner through the lifting mechanism. Through the arrangement of the technical scheme, when the sample box needs to be detected, one part of the light shielding part can slide upwards so as to facilitate the entry of the sample box.
4. The nucleic acid detection device provided by the invention is characterized in that the lifting mechanism comprises a lifting component fixed on the light shielding component and a first driving component which drives the lifting component to do lifting motion and is arranged on the rack. Through the arrangement of the technical scheme, the shading part can automatically move relative to the rack, and the control is convenient.
5. The nucleic acid detecting device provided by the invention is characterized in that at least one light absorbing layer is arranged on the outer wall surface and/or the inner wall surface of the light shielding part. Specifically, the light absorbing layer is aluminum black oxide. Through the arrangement of the technical scheme, the light absorption layer can absorb light and prevent the reflection of the light, lower background noise can be obtained during the detection of the fluorescent signal, the noise of the fluorescent signal is reduced, and the detection precision of the nucleic acid detection device is improved.
6. The nucleic acid detecting apparatus provided by the present invention further comprises a sample cartridge provided at the sample placement site; a sample is arranged on the sample placement site by being arranged in the sample cartridge; the top of the sample box is provided with detection ports which are in one-to-one correspondence with samples in the sample box; the bottom of the light shielding part is at least partially wound on the periphery of the sample box. Through the arrangement of the technical scheme, when the sample box is detected, the periphery of the sample box can be shielded by the light shielding part, external stray light is further shielded, lower background noise can be obtained during detection of the fluorescent signal, the noise of the fluorescent signal is reduced, and the detection precision of the nucleic acid detection device is improved.
7. The present invention provides a nucleic acid detecting apparatus, wherein the sample cartridge includes:
a lower cover plate having an accommodation cavity;
the upper cover plate is movably arranged on the top opening of the lower cover plate under the driving of the first driver, and when the upper cover plate is buckled on the lower cover plate, an accommodating cavity is formed by the upper cover plate and the lower cover plate; the upper cover plate is provided with the detection port. Specifically, the upper cover plate can be connected to one side of the top of the lower cover plate in a turnover mode, or the upper cover plate can be arranged on the top of the lower cover plate in a front-back sliding mode, the purpose of the invention can be achieved as long as the upper cover plate is movably connected to the lower cover plate, and by means of the arrangement of the technical scheme, the sample box can be placed and detected conveniently, and opening and closing of the upper cover plate can be controlled conveniently.
8. The invention provides a nucleic acid detection device, wherein at least one heating element is respectively arranged on the inner wall surfaces of an upper cover plate and a lower cover plate; the temperature detector and the controller are electrically connected with the temperature detector and the heating element; and/or at least one layer of heat-resistant coating is arranged on the outer wall surface of the upper cover plate and/or the lower cover plate. The corresponding heating elements are controlled by the controller to heat through sensing the temperatures of the upper cover plate and the lower cover plate by the corresponding temperature detectors respectively, so that the sample condensation phenomenon is avoided, and the accuracy of a detection result is ensured. The heat-resisting coating is black high polymer, like black Polyformaldehyde (POM), black nylon materials etc. can enough prevent that the sample box from scalding the staff to the one side high temperature of PCR board dorsad, and the black coating can not produce the reflection of light when the light source subassembly shines simultaneously, can obtain lower background noise when fluorescence signal detects, has reduced fluorescence signal's noise, has improved nucleic acid detecting device's detection precision.
9. According to the nucleic acid detection device provided by the invention, at least one heat conduction plate is further arranged on the surface of one side of the heating element, which faces away from the cover plate where the heating element is arranged; or at least one placing groove is arranged in the containing cavity of the lower cover plate, and the placing groove is used for embedding the PCR plate. Through the setting of above-mentioned technical scheme, the setting of heat-conducting plate further does benefit to heat-conduction, places the recess and is convenient for the PCR board inlays the dress, and then is convenient for aim at it and detect.
10. According to the nucleic acid detection device provided by the invention, the light shielding part is sleeved on the periphery of the rack; a sample inlet communicated with the sample placing position is formed in the side wall of the shading part;
the nucleic acid detection device also comprises a sample feeding mechanism, and the sample box is arranged on the sample feeding mechanism and driven by the sample feeding mechanism to slide along a first direction to extend into or withdraw from the sample placing position through the sample feeding port; and sliding at the sample placement location in a second direction perpendicular to the first direction. Through the arrangement of the technical scheme, the shading part can shade most of stray light, the integral sample introduction of the sample box can be realized, and the alignment of the PCR plate during detection is convenient.
11. The invention provides a nucleic acid detection device, wherein the sample injection mechanism comprises:
the first sliding mechanism is arranged on the rack and linearly slides in a reciprocating manner along a first direction;
the second sliding mechanism is arranged on the first sliding mechanism and directly slides in a reciprocating manner along a second direction;
a carrier arranged on a second slider of the second sliding mechanism; the sample cartridge is mounted on a top surface of the stage. Through the arrangement of the technical scheme, the sample introduction process of the sample box is realized, the stability of the sample introduction process is ensured, and the quality and the detection result of the sample are further ensured.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, and it is obvious that the drawings in the following description are some embodiments of the present invention, and other drawings can be obtained by those skilled in the art without creative efforts.
FIG. 1 is a schematic view of a nucleic acid detecting apparatus according to the present invention;
FIG. 2 is a schematic view of FIG. 1 with the light blocking member hidden;
FIG. 3 is a schematic view of the sample cartridge of FIG. 2 shown moved under the light source assembly;
FIG. 4 is a schematic view of a light shielding member in one direction;
FIG. 5 is a schematic diagram of the sample cartridge and sample injection mechanism of the present invention in cooperation;
FIG. 6 is a schematic view of the sample cartridge of FIG. 5;
FIG. 7 is a schematic view of a driving member of a sample cartridge according to an embodiment of the present invention;
FIG. 8 is a schematic view of a driving member of a sample cartridge according to another embodiment of the present invention;
reference numerals:
in the figure: 100-nucleic acid detection device, 1-sample box, 11-detection port, 111-rotating motor, 112-speed reducer, 113-belt wheel, 12-upper cover plate, 13-lower cover plate, 2-sample introduction mechanism, 21-carrying platform, 22-first sliding mechanism, 221-first sliding part, 23-second sliding mechanism, 231-second sliding part, 3-frame, 31-sample placement position, 4-image collector, 5-light source assembly, 6-shading assembly, 61-cover body, 62-rack, 63-gear and 64-sample introduction port.
Detailed Description
The technical solutions of the present invention will be described clearly and completely with reference to the accompanying drawings, and it should be understood that the described embodiments are some, but not all embodiments of the present invention. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
In the description of the present invention, it should be noted that the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", etc., indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, and are only for convenience of description and simplicity of description, but do not indicate or imply that the device or element being referred to must have a particular orientation, be constructed and operated in a particular orientation, and thus, should not be construed as limiting the present invention. Furthermore, the terms "first," "second," and "third" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance.
In the description of the present invention, it should be noted that, unless otherwise explicitly specified or limited, the terms "mounted," "connected," and "connected" are to be construed broadly, e.g., as meaning either a fixed connection, a removable connection, or an integral connection; can be mechanically or electrically connected; the two elements may be directly connected or indirectly connected through an intermediate medium, or may be communicated with each other inside the two elements, or may be wirelessly connected or wired connected. The specific meanings of the above terms in the present invention can be understood in specific cases to those skilled in the art.
In addition, the technical features involved in the different embodiments of the present invention described below may be combined with each other as long as they do not conflict with each other.
Examples
Referring to FIGS. 1 to 8, the present invention provides a nucleic acid detecting apparatus 100 including:
a rack 3 provided with a sample placing position 31;
the image collector 4 is arranged on the rack 3 and is positioned above the sample placing position 31;
a light source assembly 5 disposed on the frame 3 and above the sample placement site 31; the light source assembly 5 is used for irradiating a light source only for exciting fluorescence;
and the light shielding part 6 at least winds the periphery of the light emitting surface of the light source component 5 and the lens of the image collector 4 for one circle, and the bottom of the light shielding part 6 at least partially extends to the periphery of the sample placement position 31. According to the technical scheme, the sample placing position 31 can be used for placing a certain amount of samples in a certain scale, high-throughput sample detection is easy to realize, the detection time of the conventional nucleic acid detection method can be greatly shortened on the premise of ensuring the sensitivity, and the method is suitable for application scenes of large-scale nucleic acid detection; meanwhile, the arrangement of the light shielding member 6 can reduce the noise of the fluorescent signal, and improve the accuracy of the amount of the PCR reaction product reflected by the fluorescent signal, so that the detection accuracy of the nucleic acid detecting apparatus 100 is improved.
Specifically, light-blocking part 6 is a cover body 61 with an opening at the bottom, the top of cover body 61 covers image collector 4 and the top of light source component 5, and light-blocking part 6 can shield light emitted by light source component 5 and image collector 4, thereby reducing noise of fluorescent signals.
As shown in fig. 4, the light blocking member 6 is slidably provided on the rack 3 in the vertical direction by the lifting mechanism, so that when a sample cartridge 1 needs to be detected, a part of the light blocking member 6 can slide upwards to facilitate the entry of the sample cartridge 1.
Furthermore, the lifting mechanism comprises a lifting component fixed on the shading component 6 and a first driving component which drives the lifting component to do lifting motion and is arranged on the rack 3, so that the shading component 6 can automatically move relative to the rack 3 and is convenient to control.
In an embodiment of the present invention, the lifting member is a rack 62 disposed on one side of the shading member 6 along the vertical direction, the first driving component is a gear 63 fixed on the frame 3 and engaged with the rack 62, and a driving motor electrically connected to the gear 63 to drive the gear 63 to rotate, and the rack 62 is driven by driving the gear 63 to rotate, and the shading member 6 is driven to move up and down relative to the gear 63, so as to realize the automatic movement of the shading member 6.
Of course, in other embodiments, the lifting mechanism is a lifting cylinder fixed on the frame 3, and the light blocking member 6 is fixedly connected to the output end of the lifting cylinder to realize the automatic movement of the light blocking member 6. The object of the present invention can be achieved as long as the automatic movement of the light blocking member 6 can be achieved.
Preferably, at least one light absorbing layer is provided on the outer wall surface and/or the inner wall surface of the light shielding member 6. Specifically, the light-absorbing layer is black aluminum oxide, so that the light-absorbing layer can absorb light to prevent light reflection, lower background noise can be obtained during fluorescent signal detection, the noise of the fluorescent signal is reduced, and the detection precision of the nucleic acid detection device 100 is improved.
In this embodiment, as shown in FIGS. 6 to 8, the nucleic acid detecting apparatus 100 further includes a sample cartridge 1 provided on the sample placement site 31; the sample is set on the sample placement site 31 by being set in the sample cartridge 1; the top of the sample box 1 is provided with detection ports 11 which are in one-to-one correspondence with samples in the sample box; the bottom of the light shielding part 6 at least partially surrounds the periphery of the sample box 1, so that when the sample box 1 is detected, the light shielding part 6 can shield the periphery of the sample box 1, external stray light is further shielded, lower background noise can be obtained when a fluorescent signal is detected, the noise of the fluorescent signal is reduced, and the detection precision of the nucleic acid detection device 100 is improved.
The sample cartridge 1 includes: a lower cover plate 13 having a receiving cavity; the upper cover plate 12 is movably arranged on the top opening of the lower cover plate 13 under the driving of a first driver, and when the upper cover plate 12 is buckled on the lower cover plate 13, an accommodating cavity is formed between the upper cover plate 12 and the lower cover plate 13; the upper cover plate 12 is provided with the detection port 11. Specifically, the upper cover plate 12 can be connected to one side of the top of the lower cover plate 13 in a turnover manner, or the upper cover plate 12 can be arranged on the top of the lower cover plate 13 in a sliding manner, so long as the upper cover plate 12 can be movably connected to the lower cover plate 13, the purpose of the invention can be achieved.
Referring to fig. 6 and 7, in an embodiment of the present invention, the sample box 1 is provided with a rotating motor 111 respectively connected to the upper cover plate 12 and the lower cover plate 13 to drive the upper cover plate 12 and the lower cover plate 13 to open and close, so that the whole automation degree is high, and a detection sample can be well protected.
Referring to fig. 6 and 8, in another embodiment of the present invention, the sample box 1 further includes a speed reducer 112 connected to an input end of the rotating motor 111, and an output end of the speed reducer 112 is mounted with a pulley 113. Through the arrangement of the technical scheme, the service life of the rotating motor 111 can be prolonged, so that the opening and closing of the upper cover plate 12 and the lower cover plate 13 are more stable.
Specifically, at least one heating element is respectively arranged on the inner wall surfaces of the upper cover plate 12 and the lower cover plate 13; the temperature detector and the controller are electrically connected with the temperature detector and the heating element; and/or the outer wall surface of the upper cover plate 12 and/or the lower cover plate 13 is provided with at least one layer of heat-resistant coating. The corresponding heating elements are controlled by the controller to heat through the sensing of the temperatures of the corresponding temperature detectors on the upper cover plate 12 and the lower cover plate 13 respectively, so that the sample condensation phenomenon is avoided, and the accuracy of the detection result is ensured. The heat-resisting coating is black high polymer, like black Polyformaldehyde (POM), black nylon materials etc. can enough prevent that sample box 1 from scalding the staff to the one side high temperature of PCR board dorsad, and black coating can not produce the reflection of light when light source subassembly 5 shines simultaneously, can obtain lower background noise when fluorescence signal detects, has reduced fluorescence signal's noise, has improved nucleic acid detecting device 100's detection precision.
Furthermore, at least one heat conducting plate is arranged on the surface of one side of the heating element, which faces away from the cover plate on which the heating element is arranged; at least one placing groove is formed in the containing cavity of the lower cover plate 13, the placing groove is used for embedding the PCR plate, the heat conduction plate is further favorable for heat conduction, and preferably, the heat conduction plate is an aluminum plate; the placing groove is convenient for the PCR plate to be embedded and further convenient for aligning and detecting.
As shown in fig. 1 to 5, the light shielding member 6 is fitted around the outer periphery of the chassis 3; the side wall of the shading part 6 is provided with a sample inlet 64 communicated with the sample placing position 31;
the nucleic acid detecting device 100 further comprises a sample feeding mechanism 2, wherein the sample box 1 is arranged on the sample feeding mechanism 2 and driven by the sample feeding mechanism 2 to slide along a first direction to extend into or withdraw from the sample placing position 31 through the sample feeding port 64; and the second direction perpendicular to the first direction is in the position 31 is placed to the sample and slides, and light-blocking part 6 can shield most stray light, can realize the whole sample introduction of sample box 1 simultaneously, and the alignment to the PCR board when also being convenient for detect simultaneously.
The sample injection mechanism 2 comprises: a first sliding mechanism 22 which is provided on the frame 3 and linearly slides in a reciprocating manner in a first direction; a second sliding mechanism 23 which is provided in the first sliding mechanism 22 and directly slides back and forth in a second direction; a stage 21 provided on the second slider 231 of the second slide mechanism 23; the sample box 1 is arranged on the top surface of the carrying platform 21, so that the sample introduction process of the sample box 1 is realized, the stability of the sample introduction process is ensured, and the quality and the detection result of a sample are further ensured.
Specifically, in an embodiment, the first sliding mechanism 22 and the second sliding mechanism 23 each include a rotating motor and a lead screw connected to an output end of the rotating motor, the first sliding mechanism 22 has a first sliding part 221 that slides along a first direction, the second sliding mechanism 23 is fixed on the first sliding part 221, and the second sliding part 231 slides along a second direction and carries the stage 21 to move along the second direction, so that the sample introduction process of the sample cartridge 1 is implemented, and the sample introduction process is stable.
Of course, in other embodiments, the first sliding mechanism 22 and the second sliding mechanism 23 may be configured as telescopic cylinders, the first sliding mechanism 22 has a first sliding part 221 disposed at the output end of its corresponding telescopic cylinder to slide along a first direction, the second sliding mechanism 23 is fixed on the first sliding part 221, and the second sliding mechanism 23 has a second sliding part 231 disposed at the output end of its corresponding telescopic cylinder to slide along a second direction, and carry the stage 21 to move along the second direction, so as to implement the sample injection process of the sample cartridge 1, and the sample injection process is stable. The object of the present invention can be achieved as long as the moving mechanism of the stage 21 can be realized.
The detection method of the accounting detection device comprises the following steps:
controlling the upper cover plate to be opened, and correspondingly placing the PCR plate in the placing groove of the lower cover plate;
controlling the rotating motor to cover the upper cover plate, controlling the heating element to heat the upper cover plate and the lower cover plate, and controlling the heating element to heat the upper cover plate and the lower cover plate to a preset temperature according to the temperature of the temperature detector;
controlling the lifting mechanism to work so that the shading part shields the light source component and the image collector;
and controlling the sample feeding mechanism to carry the sample box into the opening on the front side of the light shield, and controlling the lifting mechanism and working to enable the light shield to shield the sample box for fluorescence signal detection.
In summary, the present invention provides a nucleic acid detecting apparatus 100, including: a rack 3 provided with a sample placing position 31; the image collector 4 is arranged on the rack 3 and is positioned above the sample placing position 31; a light source assembly 5 provided on the frame 3 and located above the sample placement position 31; the light source assembly 5 is used for irradiating a light source only for exciting fluorescence; and the light shielding part 6 at least winds the periphery of the light emitting surface of the light source component 5 and the lens of the image collector 4 for one circle, and the bottom of the light shielding part 6 at least partially extends to the periphery of the sample placement position 31. According to the technical scheme, the sample placing position 31 can be used for placing a certain amount of samples on a certain scale, so that the detection time of the conventional nucleic acid detection method can be greatly shortened on the premise of ensuring the sensitivity, and the method is suitable for application scenes of large-scale nucleic acid detection; meanwhile, the arrangement of the light shielding member 6 can reduce the noise of the fluorescent signal, and improve the accuracy of the amount of the PCR reaction product reflected by the fluorescent signal, so that the detection accuracy of the nucleic acid detecting apparatus 100 is improved.
It should be understood that the above examples are only for clarity of illustration and are not intended to limit the embodiments. Other variations and modifications will be apparent to persons skilled in the art in light of the above description. And are neither required nor exhaustive of all embodiments. And obvious variations or modifications therefrom are within the scope of the invention.
Claims (11)
1. A nucleic acid detecting apparatus, comprising:
a rack provided with a sample placing position;
the image collector is arranged on the rack and is positioned above the sample placing position;
the light source assembly is arranged on the rack and is positioned above the sample placing position; the light source assembly is used for irradiating a light source only used for exciting fluorescence;
the light shielding part at least winds the periphery of the light emitting surface of the light source component and the periphery of the lens of the image collector by one circle, and the bottom of the light shielding part at least partially extends to the periphery of the sample placing position.
2. The nucleic acid detecting device according to claim 1, wherein the light shielding member is a cover body having an opening at a bottom thereof, and a top of the cover body covers over the top of the image collector and the light source assembly.
3. The nucleic acid detecting apparatus according to claim 1, wherein the light blocking member is slidably provided on the frame in a vertical direction by an elevating mechanism.
4. The nucleic acid detecting apparatus according to claim 3, wherein the elevating mechanism includes an elevating member fixed to the light shielding member, and a first driving unit provided on the frame for driving the elevating member to move up and down.
5. The nucleic acid detecting device according to any one of claims 1 to 4, wherein at least one light absorbing layer is provided on an outer wall surface and/or an inner wall surface of the light shielding member.
6. The nucleic acid detecting apparatus according to claim 1, further comprising a sample cartridge provided at the sample placement site;
a sample is arranged on the sample placement site by being arranged in the sample cartridge; the top of the sample box is provided with detection ports which are in one-to-one correspondence with samples in the sample box;
the bottom of the light shielding part is at least partially wound on the periphery of the sample box.
7. The nucleic acid detecting apparatus according to claim 6, wherein the sample cartridge includes a lower cover plate having a housing chamber;
the upper cover plate is movably arranged on the top opening of the lower cover plate under the driving of the first driver, and when the upper cover plate is buckled on the lower cover plate, an accommodating cavity is formed by the upper cover plate and the lower cover plate; the upper cover plate is provided with the detection port.
8. The nucleic acid detecting apparatus according to claim 7, wherein at least one heating element is provided on an inner wall surface of each of the upper cover plate and the lower cover plate; the temperature detector and the controller are electrically connected with the temperature detector and the heating element; and/or
And at least one layer of heat-resistant coating is arranged on the outer wall surface of the upper cover plate and/or the lower cover plate.
9. The nucleic acid detecting device according to claim 8, wherein at least one heat conducting plate is further provided on a surface of the heating element facing away from the cover plate on which each heating element is provided; or
At least one placing groove is formed in the containing cavity of the lower cover plate, and the placing groove is used for embedding the PCR plate.
10. The nucleic acid detecting device according to any one of claims 6 to 9, wherein the light shielding member is fitted over an outer periphery of the housing; a sample inlet communicated with the sample placing position is formed in the side wall of the shading part;
the nucleic acid detection device also comprises a sample feeding mechanism, and the sample box is arranged on the sample feeding mechanism and driven by the sample feeding mechanism to slide along a first direction to extend into or withdraw from the sample placing position through the sample feeding port; and sliding at the sample placement location in a second direction perpendicular to the first direction.
11. The nucleic acid detecting device according to claim 10, wherein the sample introduction mechanism includes:
the first sliding mechanism is arranged on the rack and linearly slides in a reciprocating manner along a first direction;
the second sliding mechanism is arranged on the first sliding mechanism and directly slides in a reciprocating manner along a second direction;
a carrier arranged on a second slider of the second sliding mechanism; the sample cartridge is mounted on a top surface of the stage.
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Application publication date: 20201124 Assignee: ANHUI TONGKE BIOTECHNOLOGY Co.,Ltd. Assignor: Suzhou Institute of Biomedical Engineering and Technology Chinese Academy of Sciences Contract record no.: X2020980006484 Denomination of invention: A nucleic acid detection device License type: Common License Record date: 20200927 |
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