CN113016795B - Broad-spectrum sex attractant for bark beetles, and preparation method and application thereof - Google Patents

Broad-spectrum sex attractant for bark beetles, and preparation method and application thereof Download PDF

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CN113016795B
CN113016795B CN202110182790.XA CN202110182790A CN113016795B CN 113016795 B CN113016795 B CN 113016795B CN 202110182790 A CN202110182790 A CN 202110182790A CN 113016795 B CN113016795 B CN 113016795B
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bark beetle
bark
broad
ethanol
slow release
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CN113016795A (en
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师科
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Jieke Zhichuang Beijing Biotechnology Co ltd
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Jieke Zhichuang Beijing Biotechnology Co ltd
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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N31/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic oxygen or sulfur compounds
    • A01N31/02—Acyclic compounds
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01M—CATCHING, TRAPPING OR SCARING OF ANIMALS; APPARATUS FOR THE DESTRUCTION OF NOXIOUS ANIMALS OR NOXIOUS PLANTS
    • A01M1/00—Stationary means for catching or killing insects
    • A01M1/02—Stationary means for catching or killing insects with devices or substances, e.g. food, pheronones attracting the insects
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N27/00—Biocides, pest repellants or attractants, or plant growth regulators containing hydrocarbons
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N35/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having two bonds to hetero atoms with at the most one bond to halogen, e.g. aldehyde radical
    • A01N35/06—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having two bonds to hetero atoms with at the most one bond to halogen, e.g. aldehyde radical containing keto or thioketo groups as part of a ring, e.g. cyclohexanone, quinone; Derivatives thereof, e.g. ketals
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/90—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having two or more relevant hetero rings, condensed among themselves or with a common carbocyclic ring system
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01M—CATCHING, TRAPPING OR SCARING OF ANIMALS; APPARATUS FOR THE DESTRUCTION OF NOXIOUS ANIMALS OR NOXIOUS PLANTS
    • A01M2200/00—Kind of animal
    • A01M2200/01—Insects
    • A01M2200/012—Flying insects

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  • Life Sciences & Earth Sciences (AREA)
  • Pest Control & Pesticides (AREA)
  • Wood Science & Technology (AREA)
  • Environmental Sciences (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Plant Pathology (AREA)
  • General Health & Medical Sciences (AREA)
  • Dentistry (AREA)
  • Health & Medical Sciences (AREA)
  • Agronomy & Crop Science (AREA)
  • Insects & Arthropods (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

The invention relates to the field of biological attractants, in particular to a bark beetle broad-spectrum sex attractant and a preparation method and application thereof. The broad-spectrum sex attractant comprises bark beetle dienol (Id), cis-verbenol (S) -cis-verbenol (cV), bark beetle exo-Brevicomin (xB), ethanol (E) and alpha-pinene (P), has extremely strong attracting effect on insect adults of multiple genera in bark beetle families, has low cost and high broad-spectrum property, can exert high-efficiency attracting effect on multiple bark beetles in different working environments, and can attract partial bark beetles even without defining the pheromone, and meanwhile, the attractant has relatively strong attracting effect on other insects such as longhorners, weevilles, step beetles and the like, thereby being more beneficial to popularization and application.

Description

Broad-spectrum sex attractant for bark beetles, and preparation method and application thereof
Technical Field
The invention relates to the field of biological attractants, in particular to a bark beetle broad-spectrum sex attractant and a preparation method and application thereof.
Background
The bark beetle is a general term of Hexapoda (Hexapoda), insect (instrea), coleoptera (Coleoptera) and bark beetle (scolyidae) insects, is a small-sized beetle, has a body length of about 0.8mm to 9.0mm, is generally oblong or cylindrical, has a few hemispheres, is one of worldwide serious forest pests endangering forests, and has been described as about 220 more than 6000 species, and at present, more than 500 species are recorded in China, wherein 120 species are commonly generated. Common pests are bark beetles (Ips spp.), bark beetles (densctonus spp.), bark beetles (Xyleborus spp.), bark beetles (Hypothenemus spp.), bark beetles (pityogens spp.), etc.
The bark beetles are tiny in shape and are harmful in the interior of trees or woods, so that the bark beetles are difficult to detect and pay attention to, but the occurrence areas are very wide, and the bark beetles can be damaged by the forests and woods. Bark beetle damages the nutrition of trees and the water-dredged tissues, can cause withering and even death of the trees in a short time, and has great harm to forests. Bark beetles can be divided into two broad categories, bark beetles (bark beetles) and bark beetles (ambrosia beetles). The bark beetle directly feeds the tree tissue in the tunnel between the bark and the edge, the food is the host plant body, the selection of the host is more strict, and the bark beetle is monophagic or oligotrophic, such as bark beetle, etc.; the edible fungus bark beetle is drilled inside the xylem, the tunnel is drilled in the wood core vertically and horizontally, fungi and other microorganisms are symbiotic with the tunnel, hyphae and spores of the fungi and other microorganisms are eaten, the selection of the bark beetle to host plants is not so strict, and most of the bark beetles are of multiple eating types, such as bark beetles, bark beetles and the like.
Insect pheromones are different in sources in different insect groups, pheromones of the family bark beetle are mainly generated in the hindgut and fat tissues and released along with feces, the hindgut mainly generates isoprenoid compounds (bark beetle dienol Ipsdienol, bark beetle enol Ipsenol (Ie), bark beetle exo-Brevicomin and the like), and the fat tissues mainly generate compounds metabolized by alpha-pinene (cis-verbenol, trans-verbenol and the like); the insect pheromone can be used as a bait for trapping and killing target insects, and the population quantity of the insect pheromone is reduced so as to reduce invasion and harm to host plants. The aggregation pheromone has remarkable effect on sustainable treatment of pests, can be used by mixing pesticides with the aggregation pheromone, and can effectively trap and kill some hemiptera and coleopteran pests. Because of the large-area explosion of the bark beetles on spruce, the attractant with aggregation pheromones Id, cV and 232-MB as baits is arranged in the occurrence area of the bark beetles on Norway in 1979-1980, the effect is very remarkable, the trapping amount per year is counted in billions, and the bark beetle hazard starts to be reduced after 1980.
In the environments of forests, imported timber yards and the like, a plurality of bark beetles are frequently appeared at the same time, so that the bark beetle attractant needs to be monitored and treated at the same time, the high-efficiency broad-spectrum bark beetle attractant becomes necessary for work, the high biological activity of the used substances is ensured, the pollution is avoided, natural enemies are not killed, the resistance of pests is not caused, and no product of the bark beetle broad-spectrum attractant is seen in the market at present. Meanwhile, although attractants are also mentioned in the prior art such as CN106614751A, CN106614578A, CN106614575a, they belong to genus-level attractants for bark beetle, bark beetle and wood beetle, respectively, in turn, without having broad spectrum above bark beetle class.
Disclosure of Invention
In order to solve the problems in the prior art, the invention provides a broad-spectrum sex attractant for bark beetles, a preparation method and application thereof, and the attractant has broad-spectrum attracting effect on various bark beetles in bark beetles.
Specifically, the present invention provides a broad-spectrum sex attractant for bark beetles, comprising: bark beetle dienol Ipsdienol (Id), cis-Verbenol (S) -cis-Verbenol (cV), bark beetle exo-Brevicomin (xB), ethanol (E), α -pinene (P).
In order to further enhance the synergistic effect among the components and further facilitate the broad-spectrum trapping effect of the attractant, the invention optimizes the dosage of the components to obtain the following preferred scheme.
Preferably, the molar ratio of bark beetle dienol, cis-verbenol, bark beetle with larch size, ethanol and alpha-pinene is (0.9-1.4): 0.7-1.2): 2.0-2.5): 3410-3440): 155-185.
More preferably, the molar ratio of bark beetle dienol, cis-verbenol, bark beetle of ciliate size, ethanol and alpha-pinene is 1.143:0.985:2.2225:3425.2:170.
preferably, the bark beetle dienol, cis-verbenol, bark beetle, ethanol and alpha-pinene are respectively loaded into a slow release carrier.
More preferably, the slow release carrier is one or more of a polyethylene slow release pouch, an EP tube and an EP bottle.
Further preferably, the slow release carrier of the bark beetle dienol and the bark beetle sulbactam is a polyethylene slow release pouch and an EP tube;
the slow release carrier of the cis-verbenol and the ethanol is a polyethylene slow release pouch; preferably, the slow release carrier of the ethanol is a polyethylene slow release pouch containing cotton;
the slow release carrier of the alpha-pinene is an EP tube or an EP bottle (preferably a 16ml EP tube or an EP bottle).
Preferably, the polyethylene slow-release pouch in the slow-release carrier of cis-verbenol and xisong bark beetle is a light-tight pouch (such as a black pouch), and the polyethylene slow-release pouch in the slow-release carrier of bark beetle dienol is a transparent pouch.
The invention discovers that the matching effect among the components is better when the slow release carriers are respectively adopted, thereby further improving the broad-spectrum trapping effect.
The invention also provides a preparation method of the bark beetle broad-spectrum sex attractant, wherein bark beetle dienol, cis-verbenol, bark beetle pigment, ethanol and alpha-pinene are respectively loaded into a slow-release carrier.
As a preferred scheme, the preparation method specifically comprises the following steps:
loading bark beetle dienol and bark beetle pigment into an EP pipe respectively, covering a pipe cap, putting the EP pipe into a polyethylene slow-release small bag, discharging air in the bag, and sealing;
respectively loading cis-verbenol and ethanol into a polyethylene slow-release pouch, discharging air in the pouch, and sealing; preferably, the slow release carrier of the ethanol is a polyethylene slow release pouch containing cotton;
the alpha-pinene is loaded into an EP tube or EP bottle (preferably a 16ml EP tube or EP bottle) and then capped.
The invention also provides application of the bark beetle broad-spectrum sex attractant in monitoring and controlling bark beetle insects.
Preferably, in use, the bark beetle broad-spectrum sex attractant is provided in a trap.
As an alternative embodiment, when in use, the slow release carrier containing the five components is hung on a trapper for trapping after being strung together by iron wires.
The above schemes can be combined by a person skilled in the art to obtain a preferred embodiment of the present invention.
The invention has the beneficial effects that:
the attractant provided by the invention has extremely strong attracting effect on various insect adults of bark beetles, is low in cost and high in broad spectrum, can exert high-efficiency attracting effect on various bark beetles in different working environments, even has a part of bark beetles with unknown pheromone components, has higher attracting effect on various bark beetles in the families of bark beetles, bark beetles and bark beetles, such as weevilidae, leaf beetles, bu Jiake and the like, and has less consumption and remarkable attracting effect. In addition, the attractant has a strong attracting effect on various other insects such as longhorned beetles, weevils and step beetles, and is further beneficial to popularization and application.
Detailed Description
The following examples are illustrative of the invention and are not intended to limit the scope of the invention.
The specific techniques or conditions are not identified in the examples and are described in the literature in this field or are carried out in accordance with the product specifications. The reagents or equipment used were conventional products available for purchase by regular vendors without the manufacturer's attention.
Example 1
1. Material
Bark beetle dienol (Id), available from beijing gerbil source technologies.
Cethon bark beetle exo-Brevicomin (xB), available from Beijing Geril sources technology Co.
Cis-verbenol (cV) available from beijing grippi sources science and technology.
Ethanol (E), α -pinene (P), purchased from Sigma.
Polyethylene sustained release pouch, purchased from table city, famous plastics company, 4cm x 5cm x 20 polyethylene sustained release pouch (transparent), 4cm x 5cm x 20 polyethylene sustained release pouch (black), 10cm x 15cm x 6 polyethylene sustained release pouch (transparent).
16ml polyethylene sustained release vials purchased from beijing gerbil source technologies.
EP tube (200 μl), available from Beijing Laibunon Biotechnology Co., ltd.
2. The dosage is as follows:
the component amounts
3. The preparation method is shown in Table 1 below.
TABLE 1
Example 2
The difference between this example and example 1 is only that the amounts of bark beetle dienol, cis-verbenol, bark beetle-size bark beetle, alpha-pinene, ethanol used are different, specifically:
the component amounts
Example 3
The difference between this example and example 1 is only that the amounts of bark beetle dienol, cis-verbenol, bark beetle-size bark beetle, alpha-pinene, ethanol used are different, specifically:
the component amounts
Experimental example 1
Four attractants containing Id+xB, id+xB+cV, id+xB+P+ E, id +xB+cV+P+E were prepared separately, wherein Id, xB, cV, P, E was used in the same amount, slow release carrier and preparation method as in example 1.
The four attractants prepared were subjected to trap comparison experiments with the attractants obtained in example 1 (Id+xB+cV+P+E), each experiment was repeated 5 times, suspended in traps at intervals of 50 meters after random disruption, inspected and collected once a week, and returned to laboratory for identification. The results are shown in Table 2:
TABLE 2 comparison of types of bark beetles trapped in each group
Note that: + indicates that bark beetles are trapped.
As can be seen from Table 2, the trapping species for the simultaneous use of five compounds, bark beetle alcohol, cis-verbenol, bark beetle, alpha-pinene, ethanol, are significantly more than the trapping species for the use of a mixture of two or three compounds. The attractant composed of five compounds can trap various pests such as bark beetle, bark beetle and the like. The use of the attractant can greatly reduce the harm of external bark beetles, especially bark beetle family pests to our forests and imported wood.
In addition, the trapping effects of the embodiments 2 and 3 are detected by referring to the method, and the result shows that the trapping type of the embodiment 2 is slightly less than that of the embodiment 1, the duration of the embodiment 1 is shortened, the duration of the embodiment 1 is about 20 days, but the duration of the embodiment 2 is about 10 days, and the late-stage trapping effect is obviously reduced. But five compounds of bark beetle dienol, cis-verbenol, bark beetle extract, alpha-pinene and ethanol are used simultaneously, and have obvious advantages compared with other control groups. The trapping species of example 3 is slightly less than that of example 1, and the duration of the trapping species is similar to that of example 1, but the five compounds of bark beetle dienol, cis-verbenol, ciliate bark beetle, alpha-pinene and ethanol are used simultaneously, so that the trapping species has obvious advantages compared with other control groups. However, the amount of each component in example 3 is significantly larger than that in example 1, and example 1 is preferable from the viewpoint of economic cost.
While the invention has been described in detail in the foregoing general description and with reference to specific embodiments thereof, it will be apparent to one skilled in the art that modifications and improvements can be made thereto. Accordingly, such modifications or improvements may be made without departing from the spirit of the invention and are intended to be within the scope of the invention as claimed.

Claims (11)

1. A broad-spectrum sex attractant for bark beetles, comprising: bark beetle dienol, cis-verbenol, bark beetle-type bark beetle, ethanol and alpha-pinene;
the molar ratio of bark beetle dienol, cis-verbenol, bark beetle pigment, ethanol and alpha-pinene is (0.9-1.4): (0.7-1.2): (2.0-2.5): (3410-3440): (155-185).
2. The broad-spectrum sex attractant for bark beetle insects as claimed in claim 1, wherein the molar ratio of bark beetle dienol, cis-verbenol, bark beetle-size, ethanol, α -pinene is 1.143:0.985:2.2225:3425.2:170.
3. a broad-spectrum sex attractant for bark beetle insects as claimed in claim 1 or 2, wherein bark beetle dienol, cis-verbenol, bark beetle-size, ethanol, α -pinene are separately loaded into a slow release carrier.
4. A broad-spectrum sex attractant for bark beetle insects as claimed in claim 3, wherein said slow release carrier is one or more of a polyethylene slow release pouch, an EP tube and an EP bottle.
5. The broad-spectrum sex attractant for bark beetle insects as claimed in claim 4, wherein the slow release carriers of bark beetle dienol and bark beetle-sized bark beetle are polyethylene slow release sachets and EP tubes;
the slow release carrier of the cis-verbenol and the ethanol is a polyethylene slow release pouch;
the slow release carrier of the alpha-pinene is an EP tube or an EP bottle.
6. A broad-spectrum sex attractant for bark beetle insects as claimed in claim 5, wherein said slow release carrier of ethanol is a polyethylene slow release pouch containing cotton.
7. The method for preparing the broad-spectrum sex attractant for bark beetle insects as claimed in any one of claims 1 to 6, wherein bark beetle dienol, cis-verbenol, bark beetle-size bark beetle-element, ethanol and alpha-pinene are respectively loaded into a slow release carrier.
8. The preparation method according to claim 7, characterized by comprising the following steps:
loading bark beetle dienol and bark beetle pigment into an EP pipe respectively, covering a pipe cap, putting the EP pipe into a polyethylene slow-release small bag, discharging air in the bag, and sealing;
respectively loading cis-verbenol and ethanol into a polyethylene slow-release pouch, discharging air in the pouch, and sealing;
the alpha-pinene is loaded into an EP tube or EP bottle and then capped.
9. The method of claim 8, wherein the ethanol is loaded into a cotton-containing polyethylene slow release pouch.
10. Use of the broad-spectrum sex attractant for bark beetle insects as claimed in any of claims 1 to 6 for the monitoring and control of bark beetle insects.
11. The use according to claim 10, wherein the bark beetle insect broad-spectrum sex attractant is provided in a trap, in use.
CN202110182790.XA 2021-02-07 2021-02-07 Broad-spectrum sex attractant for bark beetles, and preparation method and application thereof Active CN113016795B (en)

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Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101390515A (en) * 2008-11-12 2009-03-25 青海省森林病虫害防治检疫总站 Aggregation pheromone attractant for attracting east false-dentate bark beetle
CN106614575A (en) * 2015-10-30 2017-05-10 中国检验检疫科学研究院 Xyleborus eichhoff attractant
CN106614751A (en) * 2015-10-30 2017-05-10 中国检验检疫科学研究院 Attractant for dendroctonus
CN106614578A (en) * 2015-10-30 2017-05-10 中国检验检疫科学研究院 Ips attractant

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101390515A (en) * 2008-11-12 2009-03-25 青海省森林病虫害防治检疫总站 Aggregation pheromone attractant for attracting east false-dentate bark beetle
CN106614575A (en) * 2015-10-30 2017-05-10 中国检验检疫科学研究院 Xyleborus eichhoff attractant
CN106614751A (en) * 2015-10-30 2017-05-10 中国检验检疫科学研究院 Attractant for dendroctonus
CN106614578A (en) * 2015-10-30 2017-05-10 中国检验检疫科学研究院 Ips attractant

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