CN113016795A - Broad-spectrum attractant for bark beetle and preparation method and application thereof - Google Patents

Broad-spectrum attractant for bark beetle and preparation method and application thereof Download PDF

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CN113016795A
CN113016795A CN202110182790.XA CN202110182790A CN113016795A CN 113016795 A CN113016795 A CN 113016795A CN 202110182790 A CN202110182790 A CN 202110182790A CN 113016795 A CN113016795 A CN 113016795A
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broad
bark
bark beetle
ethanol
verbenol
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CN113016795B (en
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师科
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Jieke Zhichuang Beijing Biotechnology Co ltd
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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N31/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic oxygen or sulfur compounds
    • A01N31/02—Acyclic compounds
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01M—CATCHING, TRAPPING OR SCARING OF ANIMALS; APPARATUS FOR THE DESTRUCTION OF NOXIOUS ANIMALS OR NOXIOUS PLANTS
    • A01M1/00—Stationary means for catching or killing insects
    • A01M1/02—Stationary means for catching or killing insects with devices or substances, e.g. food, pheronones attracting the insects
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N27/00—Biocides, pest repellants or attractants, or plant growth regulators containing hydrocarbons
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N35/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having two bonds to hetero atoms with at the most one bond to halogen, e.g. aldehyde radical
    • A01N35/06—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having two bonds to hetero atoms with at the most one bond to halogen, e.g. aldehyde radical containing keto or thioketo groups as part of a ring, e.g. cyclohexanone, quinone; Derivatives thereof, e.g. ketals
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/90—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having two or more relevant hetero rings, condensed among themselves or with a common carbocyclic ring system
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01M—CATCHING, TRAPPING OR SCARING OF ANIMALS; APPARATUS FOR THE DESTRUCTION OF NOXIOUS ANIMALS OR NOXIOUS PLANTS
    • A01M2200/00—Kind of animal
    • A01M2200/01—Insects
    • A01M2200/012—Flying insects

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  • Life Sciences & Earth Sciences (AREA)
  • Pest Control & Pesticides (AREA)
  • Wood Science & Technology (AREA)
  • Environmental Sciences (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Plant Pathology (AREA)
  • General Health & Medical Sciences (AREA)
  • Dentistry (AREA)
  • Health & Medical Sciences (AREA)
  • Agronomy & Crop Science (AREA)
  • Insects & Arthropods (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

The invention relates to the field of biological attractants, and in particular relates to a broad-spectrum attractant for bark beetles, and a preparation method and application thereof. The broad-spectrum attractant for the bark beetle comprises bark beetle dienol Ipsdienol (Id), cis-verbenol (S) -cis-verbenol (cV), Cedar bark beetle exo-Brevicomin (xB), ethanol (E) and alpha-pinene (P), has strong trapping effect on insect adults of multiple genera in the bark beetle, has low cost and high broad-spectrum property, can play a high-efficiency trapping effect on multiple bark beetles in different working environments, and can trap partial bark beetles even not clearly identify pheromones of the bark beetles.

Description

Broad-spectrum attractant for bark beetle and preparation method and application thereof
Technical Field
The invention relates to the field of biological attractants, and in particular relates to a broad-spectrum attractant for bark beetles, and a preparation method and application thereof.
Background
The bark beetle is a general name of insects in the Hexapoda (Hexapoda), Insecta (Insecta), Coleoptera (Coleoptera) and bark beetle (Scolytidae), is a small beetle, has the body length of about 0.8mm-9.0mm, is generally in an oblong shape or a cylindrical shape, has a small number of hemispheres, is one of the worldwide important forest pests harming forest trees, has described about 220 to 6000 species so far, records more than 500 species in China at present, and has more than 120 species in common occurrence. Common genera are Ips (Ips spp.), pissodes piscioides (Dendroctonus spp.), pissodes piscioides (Xyleborus spp.), pissodes piscioides (Hypothenemus spp.), pissodes piscicola (Hypothenemus spp.), pissodes punctatus (Pitylenes spp.), and the like.
The bark beetle is tiny in shape and is harmful inside trees or woods, so the bark beetle is difficult to detect and attract attention, but the bark beetle is widely generated in areas, and can be damaged by the bark beetle as long as the bark beetle is in forests and woods. Bark beetles destroy tree nutrition and destroy water dredging tissues, can cause withering and even death of trees in a short time, and have great harm to forests. Bark beetles can be divided into two major classes, bark beetles (bark beetles) and fungi bark beetles (ambrosia beetles). Bark beetles are in tunnels between bark and saplings, directly eat tree tissues, and food is a host plant body, and the selection of the food to the host is strict and is mostly single-feeding or low-feeding, such as large bark beetles, dentate bark beetles, star pit bark beetles and the like; the housefly bark beetles are bored in the xylem, tunnels penetrate through wood cores in a longitudinal and transverse mode, fungi and other microorganisms coexist with the wood cores, hypha and spores of the fungi and the other microorganisms are eaten, the selection of the housefly bark beetles on host plants is not strict, and the housefly bark beetles are mostly of multi-feeding types, such as bark beetles, wood bark beetles and the like.
Insect pheromones are different in sources in different insect groups, the pheromones of the family bark beetle are mainly produced in the rear intestine and fat tissues and released with feces, isoprenoid compounds (bark beetle dienol Ipsdienol, bark beetle alcohol Ipsenol (ie), Cesone big bark beetle exo-Brevicomin and the like) are mainly produced in the rear intestine, and compounds metabolized by alpha-pinene (cis-verbenol, trans-verbenol, and the like) are mainly produced in the fat tissues; the insect pheromone can be used as a bait for trapping and killing target insects, and the population quantity of the insect pheromone is reduced so as to reduce the invasion and harm to host plants. The aggregation pheromone has obvious effect on sustainable management of pests, and the pesticide and the aggregation pheromone can be mixed for use, so that certain hemiptera and coleopteran pests can be effectively trapped and killed. Due to the large-area outbreak of ips typographus, the gathering pheromones Id, cV and 232-MB are set as attractants in the occurrence area in 1979-1980 in Norway, the effect is very remarkable, the trapping amount is billions each year, and after 1980, the damage of ips typographus begins to be reduced.
The broad-spectrum ips attractant is used for monitoring and controlling various ips in forest, imported wood stacking places and other environments frequently, and has the advantages that the broad-spectrum ips attractant with high efficiency is necessary for work, high biological activity, no pollution, no killing of natural enemies and no resistance to pests are guaranteed, but no product of the broad-spectrum ips attractant is available in the market at present. Meanwhile, although the attractants are also mentioned in the prior arts such as CN106614751A, CN106614578A and CN106614575A, the attractants respectively belong to genus level attractants for the genus pissodes, the genus ips and the genus pissodes in turn, and do not have broad spectrum of above the family level of pissodes.
Disclosure of Invention
In order to solve the problems in the prior art, the invention provides a broad-spectrum attractant for bark beetles, a preparation method and application thereof.
Specifically, the invention firstly provides a broad-spectrum attractant for bark beetle, which comprises the following components: ips dienol (Id), cis-verbenol (S) -cis-Verbenol (cV), cembrevicin exo-Brevicomin (xB), ethanol (E), alpha-pinene (P).
In order to further enhance the synergistic effect among the components and to be more beneficial to the broad-spectrum trapping effect of the attractant, the dosage of the components is optimized, and the following preferred scheme is obtained.
Preferably, the molar ratio of bark beetle dienol, cis-verbenol, Cedar bark beetle, ethanol and alpha-pinene is (0.9-1.4): (0.7-1.2): (2.0-2.5): (3410-3440): 155-185).
More preferably, the mole ratio of bark beetle dienol, cis-verbenol, cembrene, ethanol and alpha-pinene is 1.143: 0.985: 2.2225: 3425.2: 170.
preferably, the bark beetle dienol, cis-verbenol, cembrene bark beetle, ethanol and alpha-pinene are loaded into the slow release carrier respectively.
More preferably, the slow release carrier is one or more of a polyethylene slow release pouch, an EP tube and an EP bottle.
Further preferably, the sustained-release carrier of the bark beetle dienol and the bristletail is a polyethylene sustained-release small bag and an EP pipe;
the slow release carrier of the cis-verbenol and the ethanol is a polyethylene slow release small bag; preferably, the slow release carrier of the ethanol is a polyethylene slow release pouch containing cotton;
the slow release carrier of the alpha-pinene is an EP tube or an EP bottle (preferably 16ml EP tube or EP bottle).
Preferably, the polyethylene slow-release pouch in the slow-release carrier of cis-verbena enol and cembrene bristletin is a light-tight pouch (e.g., a black pouch), and the polyethylene slow-release pouch in the slow-release carrier of bristledienol can be a transparent pouch.
The invention discovers that when the sustained-release carriers are respectively adopted, the matching effect of the components is better, thereby further improving the broad-spectrum trapping effect.
The invention also provides a preparation method of the bark beetle broad-spectrum attractant, which loads bark beetle dienol, cis-verbenol, cembrene bark beetle, ethanol and alpha-pinene into a slow release carrier respectively.
As a preferred scheme, the preparation method specifically comprises the following steps:
loading bark beetle dienol and cembrene into EP tube, covering tube cap, placing EP tube into polyethylene slow release small bag, discharging air, and sealing;
respectively loading cis-verbenol and ethanol into the polyethylene slow-release small bag, discharging air out of the bag, and sealing; preferably, the slow release carrier of the ethanol is a polyethylene slow release pouch containing cotton;
the alpha-pinene is loaded into an EP tube or an EP bottle (preferably 16ml EP tube or EP bottle) and sealed.
The invention also provides application of the broad-spectrum attractant for bark beetles in monitoring and treating bark beetle insects.
Preferably, in use, the broad-spectrum attractant for the bark beetle is disposed in the trap.
As an alternative embodiment, in use, the slow release carrier containing the five components is strung together by iron wires and then hung on a trap for trapping.
The above-described schemes can be combined by those skilled in the art to obtain the preferred embodiments of the present invention.
The invention has the beneficial effects that:
the attractant provided by the invention has extremely strong trapping effect on various adult insects in the family of bark beetles, is low in cost and high in broad spectrum, can simultaneously play a role in efficiently trapping various bark beetles in different working environments, even part of bark beetles with undefined pheromone components have higher trapping effect on various bark beetles in the families of bark beetles, weevil, phyllocnide, amberziaceae and the like, and has less dosage and obvious trapping effect. In addition, the attractant in the invention also has strong attraction effect on other insects such as longicorn, weevil, walker and the like, thereby being more beneficial to popularization and application.
Detailed Description
The following examples are intended to illustrate the invention but are not intended to limit the scope of the invention.
The examples do not show the specific techniques or conditions, according to the technical or conditions described in the literature in the field, or according to the product specifications. The reagents or instruments used are conventional products available from regular distributors, not indicated by the manufacturer.
Example 1
1. Material
Bark beetle dienol (Id) available from Beijing Gray Biyuan scientific Co.
Cedar bark beetle exo-Brevicomin (xB), available from Beijing Gray Biyuan science and technology, Inc.
Cis-verbenol (cV), available from scientific ltd, beijing griffi bei.
Ethanol (E), α -pinene (P), purchased from Sigma.
Polyethylene sustained release sachets available from taizhou city famous science limited, 4cm x 5cm x 20 polyethylene sustained release sachets (clear), 4cm x 5cm x 20 polyethylene sustained release sachets (black), 10cm x 15cm x 6 polyethylene sustained release sachets (clear).
16ml polyethylene slow release vials from Beijing Gray's source technology, Inc.
EP tubes (200. mu.l) were purchased from Biotech, Inc. of Beijing Leibo Runck.
2. The following amounts were used:
the amount of the components
Figure BDA0002941879100000051
3. The preparation method is shown in the following table 1.
TABLE 1
Figure BDA0002941879100000052
Example 2
The difference between this example and example 1 is only that the amounts of bark beetle dienol, cis-verbenol, cembrene, alpha-pinene and ethanol are different, specifically:
the amount of the components
Figure BDA0002941879100000061
Example 3
The difference between this example and example 1 is only that the amounts of bark beetle dienol, cis-verbenol, cembrene, alpha-pinene and ethanol are different, specifically:
the amount of the components
Figure BDA0002941879100000062
Experimental example 1
Four attractants containing Id + xB, Id + xB + cV, Id + xB + P + E, Id + xB + cV + P + E were prepared separately, wherein the amounts of Id, xB, cV, P, E used, the slow release vehicle and the preparation method were the same as in example 1.
The prepared four attractants were subjected to trapping comparative experiments with the attractant obtained in example 1 (Id + xB + cV + P + E), each experiment was repeated 5 times, suspended in traps at intervals of 50 meters after any arbitrary disordering sequence, collected by weekly check, and brought back to the laboratory for identification. The results are shown in Table 2:
TABLE 2 control table of bark beetle species trapped in each group
Figure BDA0002941879100000063
Figure BDA0002941879100000071
Note: + means trapping to bark beetle.
As can be seen from Table 2, the trapping species of the five compounds of bark beetle dienol, cis-verbenol, cembrene, alpha-pinene and ethanol used simultaneously are obviously more than the trapping species of the two or three compounds used in combination. The attractant composed of the five compounds can trap various pests such as ips, bark beetles and the like. The use of the attractant can greatly reduce the damage of the foreign bark beetles, particularly the bark beetle pests to forest and imported wood.
In addition, the invention also refers to the above method to detect the trapping effect of the examples 2 and 3, the result shows that the trapping species of the example 2 is slightly less than the example 1, and the effective period is shortened, the effective period of the example 1 is about 20 days, but the effective period of the example 2 is about 10 days, and the late stage trapping effect is obviously reduced. However, the combination of bark beetle dienol, cis-verbenol, cembrene, alpha-pinene and ethanol has obvious advantages compared with other control groups. The trapping species of the example 3 is slightly less than that of the example 1, the lasting period is similar to that of the example 1, but the five compounds of bark beetle dienol, cis-verbenol, bark beetle, alpha-pinene and ethanol have obvious advantages compared with other control groups when being used simultaneously. However, the amount of each component used in example 3 is significantly larger than that used in example 1, and example 1 is preferred from the viewpoint of economic cost.
Although the invention has been described in detail hereinabove with respect to a general description and specific embodiments thereof, it will be apparent to those skilled in the art that modifications or improvements may be made thereto based on the invention. Accordingly, such modifications and improvements are intended to be within the scope of the invention as claimed.

Claims (10)

1. The broad-spectrum attractant for bark beetles is characterized by comprising the following components: bark beetle dienol, cis-verbenol, cembrene bark beetle, ethanol and alpha-pinene.
2. The broad-spectrum attractant for ips according to claim 1, wherein the molar ratio of ips dienol, cis-verbenol, cembrene, ethanol, alpha-pinene is (0.9-1.4): (0.7-1.2): (2.0-2.5): (3410-3440): (155-185).
3. The ips broad-spectrum attractant of claim 2, wherein the molar ratio of ips dienol, cis-verbenol, cembrene, ethanol, alpha-pinene is 1.143: 0.985: 2.2225: 3425.2: 170.
4. the broad-spectrum attractant for ips according to any one of claims 1 to 3, wherein the ips dienol, cis-verbenol, cembrene, ethanol, alpha-pinene are loaded into slow-release carriers, respectively.
5. The bark beetle broad-spectrum attractant according to claim 4, wherein the slow release carrier is one or more of a polyethylene slow release pouch, an EP tube and an EP bottle.
6. The ips broad-spectrum attractant of claim 5, wherein the slow-release carrier of ips dienol, cembrene piscidin is a polyethylene slow-release pouch and an EP tube;
the slow release carrier of the cis-verbenol and the ethanol is a polyethylene slow release small bag; preferably, the slow release carrier of the ethanol is a polyethylene slow release pouch containing cotton;
the slow release carrier of the alpha-pinene is an EP tube or an EP bottle.
7. The method for preparing the broad-spectrum attractant for bark beetle according to any one of claims 1 to 6, wherein bark beetle dienol, cis-verbenol, cembrenoid, ethanol and alpha-pinene are loaded into a slow release carrier respectively.
8. The preparation method according to claim 7, which specifically comprises:
loading bark beetle dienol and bristletin into an EP tube respectively, covering a tube cap, putting the EP tube into a polyethylene slow-release small bag, discharging air in the bag, and sealing the bag;
respectively loading cis-verbenol and ethanol into the polyethylene slow-release small bag, discharging air out of the bag, and sealing; preferably, the slow release carrier of the ethanol is a polyethylene slow release pouch containing cotton;
loading alpha-pinene into EP tube or EP bottle, and sealing.
9. The use of the broad-spectrum attractant for bark beetle according to any one of claims 1 to 6 for the monitoring and control of bark beetle insects.
10. The use according to claim 9, wherein, in use, the broad-spectrum attractant for bark beetle is disposed in a trap.
CN202110182790.XA 2021-02-07 2021-02-07 Broad-spectrum sex attractant for bark beetles, and preparation method and application thereof Active CN113016795B (en)

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Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101390515A (en) * 2008-11-12 2009-03-25 青海省森林病虫害防治检疫总站 Aggregation pheromone attractant for attracting east false-dentate bark beetle
CN106614575A (en) * 2015-10-30 2017-05-10 中国检验检疫科学研究院 Xyleborus eichhoff attractant
CN106614751A (en) * 2015-10-30 2017-05-10 中国检验检疫科学研究院 Attractant for dendroctonus
CN106614578A (en) * 2015-10-30 2017-05-10 中国检验检疫科学研究院 Ips attractant

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101390515A (en) * 2008-11-12 2009-03-25 青海省森林病虫害防治检疫总站 Aggregation pheromone attractant for attracting east false-dentate bark beetle
CN106614575A (en) * 2015-10-30 2017-05-10 中国检验检疫科学研究院 Xyleborus eichhoff attractant
CN106614751A (en) * 2015-10-30 2017-05-10 中国检验检疫科学研究院 Attractant for dendroctonus
CN106614578A (en) * 2015-10-30 2017-05-10 中国检验检疫科学研究院 Ips attractant

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