CN113005029A - Soil microorganism respiration measuring device - Google Patents

Soil microorganism respiration measuring device Download PDF

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CN113005029A
CN113005029A CN202110263904.3A CN202110263904A CN113005029A CN 113005029 A CN113005029 A CN 113005029A CN 202110263904 A CN202110263904 A CN 202110263904A CN 113005029 A CN113005029 A CN 113005029A
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CN113005029B (en
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王焕炯
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Institute of Geographic Sciences and Natural Resources of CAS
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Abstract

The invention belongs to the technical field of soil environment monitoring, and particularly relates to a soil microorganism respiration measuring device. The measuring device comprises a box body, a rotary sample rack, a refrigerating device, a heating device, a nitrogen source, an exhaust pipe and a control module; the rotary sample rack, the refrigerating device and the heating device are arranged in the box body; the control module comprises a PLC (programmable logic controller), a temperature sensor, a carbon dioxide sensor, a plurality of electromagnetic valves and a touch screen; the PLC is in signal connection with the temperature sensor, the carbon dioxide sensor and the plurality of electromagnetic valves, and the touch screen is connected with the PLC; the PLC and the touch screen are arranged at the top of the box body, and the temperature sensor and the carbon dioxide sensor are arranged in the box body; the plurality of electromagnetic valves are respectively arranged on the connecting pipe and the exhaust pipe. The device avoids uncontrollable factors in the experiment operation of the traditional method and reduces the pollution rate of the sample.

Description

Soil microorganism respiration measuring device
Technical Field
The invention belongs to the technical field of soil environment monitoring, and particularly relates to a soil microorganism respiration measuring device.
Background
The soil respiration is the exchange process of carbon dioxide between soil and the atmosphere, directly reflects the turnover speed of carbon elements in the soil, and the micro change of the soil respiration can obviously affect the global carbon balance, even is related to the whole atmospheric environment, so that the soil respiration is an indispensable part in the whole soil ecosystem. In view of the importance of soil respiration, the change trend of some main parameters in the soil environment is predicted, such as the positive correlation relationship between the soil respiration and the soil fertility, namely, the higher the soil fertility is, the stronger the soil respiration is, and the soil fertility level can be effectively judged. According to the traditional method for measuring the soil microbial respiration, a soil respiration bottle can only be placed in an environment with carbon dioxide of about 400ppm for culture, the pollution is frequent, the experimental result is not ideal, the culture temperature of each sample is different during the culture period, the error of the experimental result is large, the sample is taken out necessarily when air is blown each time, and the sample is placed back for culture after air is blown, so that the time and the labor are wasted.
Chinese utility model patent CN206609782U discloses a soil microorganism breath measuring device, including measuring chamber, the sealed lid of measuring chamber, infrared CO2The measuring chamber consists of a shell, a metal insertion cylinder and a metal insertion plate, the metal insertion cylinder is hermetically connected with the shell, the side wall of the shell is provided with a side wall support neck, and the side wall support neck is hermetically connected with a rubber cap and adopts a closed structure. However, this patent cannot adjust the state of the measurement chamber and cannot ensure the stability of the measurement conditions.
Disclosure of Invention
The invention aims to provide a soil microorganism respiration measuring device, which avoids uncontrollable factors in the experiment operation of the traditional method, particularly reduces the pollution rate of a sample, and eliminates errors caused by non-uniform experiment conditions (mainly temperature and carbon dioxide concentration) in the experiment process.
In order to achieve the purpose, the invention adopts the following technical scheme:
a soil microorganism respiration measuring device comprises a box body, a rotary sample rack, a refrigerating device, a heating device, a nitrogen source, an exhaust pipe and a control module;
a baffle plate is arranged in the box body, the baffle plate divides an inner cavity of the box body into an upper cavity and a lower cavity, the rotary sample is arranged in the upper cavity, and a nitrogen source is communicated with the upper cavity and the sample bottle of the box body through a connecting pipe; the upper cavity is communicated with the external space of the box body through an exhaust pipe;
the refrigerating device comprises a refrigerator and a refrigerating pipe, the refrigerator is arranged in the lower cavity, and the refrigerating pipe is arranged on the side wall of the upper cavity;
the heating device comprises a heater and a heating pipe, the heater is arranged in the lower cavity, and the heating pipe is arranged on the side wall of the upper cavity;
the control module comprises a PLC (programmable logic controller), a temperature sensor, a carbon dioxide sensor, a plurality of electromagnetic valves and a touch screen; the PLC is in signal connection with the temperature sensor, the carbon dioxide sensor and the plurality of electromagnetic valves, and the touch screen is connected with the PLC;
the PLC and the touch screen are arranged at the top of the box body, and the temperature sensor and the carbon dioxide sensor are arranged in the box body; the plurality of electromagnetic valves are respectively arranged on the connecting pipe and the exhaust pipe.
As an improvement of the above technical solution, the rotary sample holder comprises a support, a plurality of sample trays, a plurality of gas distribution trays and a rotary driving mechanism; the pillar is worn to locate on the baffle, and rotary driving mechanism sets up in cavity down, the pillar is connected with rotary driving mechanism drive, and a plurality of sample dishes are fixed on the pillar with a plurality of gas distribution dish are the layered structure, and each sample dish upper surface corresponds and sets up a gas distribution dish.
As the improvement of the technical proposal, the sample disk is circular, and a circle of round holes for placing sample bottles are arranged on the sample disk.
As an improvement of the technical scheme, the air distribution disc is circular, and a plurality of air distribution holes for penetrating the connecting pipe are formed in the air distribution disc.
As an improvement of the above technical solution, the connecting pipe comprises a first nitrogen branch pipe, a second nitrogen branch pipe and a gas distribution pipe; a nitrogen outlet of the nitrogen source is connected with a first nitrogen branch pipe and a second nitrogen branch pipe, the first nitrogen branch pipe is communicated with the sample bottle, the second nitrogen branch pipe is connected with a gas distribution pipe, and the gas distribution pipe is arranged on the partition plate; the first nitrogen branch pipe and the second nitrogen branch pipe are respectively provided with an electromagnetic valve.
As an improvement of the technical scheme, the air distribution pipe is provided with a plurality of nitrogen air outlet holes at equal intervals.
As a further improvement of the above technical solution, the PLC controller is remotely connected to the user terminal through a long-distance communication manner.
As an improvement of the above technical solution, the user terminal is a mobile phone, a computer, or a tablet computer.
The box body of the device can be provided with a box body door for taking and placing sample bottles and the like.
According to the invention, the temperature sensor is arranged, so that the internal temperature of the equipment can be accurately controlled, and the use requirements of experiments at different temperatures can be met. The carbon dioxide sensor is provided to control the concentration of carbon dioxide inside the apparatus. By arranging the rotary sample disc, the temperature of each sample can be consistent. Automatic operation and mobile phone operation and reminding can be realized by arranging the touch screen, the mobile phone, the PLC and the remote module and software contained in the PLC.
The working principle of the invention is as follows:
after the soil sample is weighed, the soil sample is placed into a soil respiration sample bottle, a cover is covered, the soil sample bottle is placed on a sample rack, an upper nitrogen pipe is connected, a valve is opened, air blowing is started, and after the air blowing is finished, a corresponding electromagnetic valve is closed, and a device door is closed. According to the set parameters, the device can automatically operate, and the reliability and accuracy of data are guaranteed.
The principle of controlling carbon dioxide in the box body is as follows:
assuming that the concentration of carbon dioxide in the box body is set to be 50ppm, when the concentration of the carbon dioxide in the box body is greater than 50ppm, the carbon dioxide sensor gives a signal, the PLC receives the signal and simultaneously opens the electromagnetic valve on the nitrogen pipe and the exhaust electromagnetic valve of the box body, nitrogen gas is filled into the box body, redundant carbon dioxide is exhausted out of the room through the exhaust pipe, when the concentration of the carbon dioxide in the box body reaches 50ppm, the carbon dioxide sensor gives the signal, and the PLC receives the signal and simultaneously closes the electromagnetic valve on the nitrogen pipe and the exhaust electromagnetic valve of the box body, so that the concentration of the carbon dioxide in the box body can be.
The invention can also culture soil, adjust the water content of the soil, add glucose and put the soil into a sample bottle, and control the oxygen concentration and the carbon dioxide concentration in the device.
Compared with the prior art, the invention has the technical advantages that:
the invention avoids uncontrollable factors in the experiment operation of the traditional method, particularly reduces the pollution rate of the sample, and eliminates errors caused by non-uniform experiment conditions (mainly temperature and carbon dioxide concentration) in the experiment process. And the difficult problem that the concentration of carbon dioxide is difficult to master and control in the culture process is solved. The sample blowing link is completely in a closed-loop operation state, and sample pollution caused by repeated manual taking-out operation is completely eliminated. The sample assay can be performed in bulk. The time cost is greatly saved, and the working efficiency is improved.
Drawings
FIG. 1 is a schematic view showing the structure of a soil microorganism respiration measuring apparatus according to the present invention;
reference numerals:
1. a box body; 2. a partition plate; 3. an upper cavity; 4. a lower cavity; 5. a nitrogen source; 6. an exhaust pipe; 7. a sample bottle; 8. a refrigerator; 9. a refrigeration pipe; 10. a heater; 11. heating a tube; 12. a PLC controller; 13. a temperature sensor; 14. a carbon dioxide sensor; 15. an electromagnetic valve; 16. a touch screen; 17. a pillar; 18. a sample tray; 19. a gas distribution plate; 20. a rotation driving mechanism; 21. a circular hole; 22. air distributing holes; 23. a first nitrogen branch pipe; 24. a second nitrogen branch pipe; 25. an air distribution pipe; 26. a nitrogen gas outlet; 27. a user terminal.
Detailed Description
The invention is described in further detail below with reference to the figures and the detailed description.
Example 1
As shown in figure 1, the soil microorganism respiration measuring device comprises a box body 1, a rotary sample rack, a refrigerating device, a heating device, a nitrogen source 5, an exhaust pipe 6 and a control module;
a clapboard 2 is arranged in the box body 1, the clapboard 2 divides the inner cavity of the box body 1 into an upper cavity 3 and a lower cavity 4, the rotary sample is arranged in the upper cavity 3, and a nitrogen source 5 is communicated with the upper cavity 3 and a sample bottle 7 of the box body 1 through a connecting pipe; the upper cavity 3 is communicated with the external space of the box body 1 through an exhaust pipe 6;
the refrigerating device comprises a refrigerator 8 and a refrigerating pipe 9, the refrigerator 8 is arranged in the lower cavity 4, and the refrigerating pipe 9 is arranged on the side wall of the upper cavity 3;
the heating device comprises a heater 10 and a heating pipe 11, the heater 10 is arranged in the lower cavity 4, and the heating pipe 11 is arranged on the side wall of the upper cavity 3;
the control module comprises a PLC (programmable logic controller) 12, a temperature sensor 13, a carbon dioxide sensor 14, a plurality of electromagnetic valves 15 and a touch screen 16; the PLC 12 is in signal connection with a temperature sensor 13, a carbon dioxide sensor 14 and a plurality of electromagnetic valves 15, and the touch screen 16 is connected with the PLC 12;
the PLC 12 and the touch screen 16 are arranged at the top of the box body 1, and the temperature sensor 13 and the carbon dioxide sensor 14 are arranged inside the box body 1; the plurality of solenoid valves 15 are respectively provided on the connection pipe and the exhaust pipe 6.
The rotary sample rack comprises a supporting column 17, a plurality of sample disks 18, a plurality of gas distribution disks 19 and a rotary driving mechanism 20; the pillar 17 is arranged on the partition board 2 in a penetrating manner, the rotary driving mechanism is arranged in the lower cavity 4, the pillar 17 is in driving connection with the rotary driving mechanism 20, the sample trays 18 and the gas distribution trays 19 are fixed on the pillar 17 in a layered structure, and each sample tray 18 is correspondingly provided with one gas distribution tray 19.
The sample plate 18 is circular and is provided with a circular hole 21 for receiving a sample bottle.
The air distribution plate 19 is circular, and a plurality of air distribution holes 22 for penetrating the connecting pipes are arranged on the air distribution plate.
The connecting pipe comprises a first nitrogen branch pipe 23, a second nitrogen branch pipe 24 and a gas distribution pipe 25; a nitrogen outlet of the nitrogen source 5 is connected with a first nitrogen branch pipe 23 and a second nitrogen branch pipe 24, the first nitrogen branch pipe 23 is communicated with the sample bottle 7, the second nitrogen branch pipe 24 is connected with a gas distribution pipe 25, and the gas distribution pipe 25 is arranged on the partition plate 2; the first nitrogen branch pipe 23 and the second nitrogen branch pipe 24 are respectively provided with an electromagnetic valve 15.
A plurality of nitrogen outlet holes 26 are arranged on the air distribution pipe 25 at equal intervals.
The PLC controller 12 is remotely connected to the user terminal 27 by long-distance communication.
The user terminal 27 is a mobile phone, a computer or a tablet computer.
Conventional technical knowledge in the art can be used for the details which are not described in the present invention.
Finally, it should be noted that the above embodiments are only used for illustrating the technical solutions of the present invention and are not limited. Although the present invention has been described in detail with reference to the embodiments, it will be understood by those skilled in the art that various changes may be made and equivalents may be substituted without departing from the spirit and scope of the invention as defined in the appended claims.

Claims (8)

1. The soil microorganism respiration measuring device is characterized by comprising a box body (1), a rotary sample rack, a refrigerating device, a heating device, a nitrogen source (5), an exhaust pipe (6) and a control module;
a baffle plate (2) is arranged in the box body (1), the baffle plate (2) divides an inner cavity of the box body (1) into an upper cavity (3) and a lower cavity (4), the rotary sample is arranged in the upper cavity (3), and a nitrogen source (5) is communicated with the upper cavity (3) of the box body (1) and a sample bottle (7) through a connecting pipe; the upper cavity (3) is communicated with the external space of the box body (1) through an exhaust pipe (6);
the refrigerating device comprises a refrigerator (8) and a refrigerating pipe (9), the refrigerator (8) is arranged in the lower cavity (4), and the refrigerating pipe (9) is arranged on the side wall of the upper cavity (3);
the heating device comprises a heater (10) and a heating pipe (11), the heater (10) is arranged in the lower cavity (4), and the heating pipe (11) is arranged on the side wall of the upper cavity (3);
the control module comprises a PLC (programmable logic controller) controller (12), a temperature sensor (13), a carbon dioxide sensor (14), a plurality of electromagnetic valves (15) and a touch screen (16); the PLC (12) is in signal connection with the temperature sensor (13), the carbon dioxide sensor (14) and the electromagnetic valves (15), and the touch screen (16) is connected with the PLC (12);
the PLC controller (12) and the touch screen (16) are arranged at the top of the box body (1), and the temperature sensor (13) and the carbon dioxide sensor (14) are arranged inside the box body (1); the electromagnetic valves (15) are respectively arranged on the connecting pipe and the exhaust pipe (6).
2. A soil microbial respiration assay device according to claim 1, wherein said rotating sample holder comprises a support column (17), a plurality of sample trays (18), a plurality of gas distribution trays (19) and a rotary drive mechanism (20); the support column (17) penetrates through the partition plate (2), the rotary driving mechanism is arranged in the lower cavity (4), the support column (17) is in driving connection with the rotary driving mechanism (20), the plurality of sample trays (18) and the plurality of gas distribution trays (19) are of a layered structure and are fixed on the support column (17), and one gas distribution tray (19) is correspondingly arranged on each sample tray (18).
3. A soil microbial respiration determination apparatus as claimed in claim 2 wherein the sample plate (18) is circular and a circular aperture (21) is provided in the plate for receiving a bottle of sample.
4. The soil microorganism respiration measuring device according to claim 2, wherein the air distribution plate (19) is circular and is provided with a plurality of air distribution holes (22) for passing through the connecting pipe.
5. The soil microorganism respirometry device as recited in claim 1, wherein said connection tube comprises a first nitrogen branch tube (23), a second nitrogen branch tube (24), and a gas distribution tube (25); a nitrogen outlet of the nitrogen source (5) is connected with a first nitrogen branch pipe (23) and a second nitrogen branch pipe (24), the first nitrogen branch pipe (23) is communicated with the sample bottle (7), the second nitrogen branch pipe (24) is connected with a gas distribution pipe (25), and the gas distribution pipe (25) is arranged on the partition plate (2); the first nitrogen branch pipe (23) and the second nitrogen branch pipe (24) are respectively provided with an electromagnetic valve (15).
6. A soil microorganism respiration measuring device according to claim 1, wherein said gas distribution pipe (25) is provided with a plurality of nitrogen gas outlet holes (26) at equal intervals.
7. A soil microbial respiration determination apparatus according to any one of claims 1-6, wherein said PLC controller (12) is remotely connected to a user terminal (27) by means of long-range communication.
8. Soil microbial respiration assay device according to claim 7, characterized in that said user terminal (27) is a mobile phone, a computer or a tablet.
CN202110263904.3A 2021-03-11 2021-03-11 Soil microorganism respiration measuring device Active CN113005029B (en)

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Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102586097A (en) * 2012-01-12 2012-07-18 中国科学院地理科学与资源研究所 Device of continuously testing indoor soil microbial respiration
KR101345254B1 (en) * 2012-10-31 2013-12-26 한국화학연구원 Measurement system for soil microorganisms activity and method therefor
CN205449914U (en) * 2016-04-01 2016-08-10 中国矿业大学 Soil basis respiration monitoring culture apparatus
CN106405055A (en) * 2016-11-01 2017-02-15 青岛大学 System and method for continuous online measurement of soil CO2 flux
CN206609782U (en) * 2017-08-25 2017-11-03 济南大学 A kind of edaphon respiration measuring device

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102586097A (en) * 2012-01-12 2012-07-18 中国科学院地理科学与资源研究所 Device of continuously testing indoor soil microbial respiration
KR101345254B1 (en) * 2012-10-31 2013-12-26 한국화학연구원 Measurement system for soil microorganisms activity and method therefor
CN205449914U (en) * 2016-04-01 2016-08-10 中国矿业大学 Soil basis respiration monitoring culture apparatus
CN106405055A (en) * 2016-11-01 2017-02-15 青岛大学 System and method for continuous online measurement of soil CO2 flux
CN206609782U (en) * 2017-08-25 2017-11-03 济南大学 A kind of edaphon respiration measuring device

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